CN107523534B - 重组cho细胞的贴壁微载体悬浮培养方法 - Google Patents
重组cho细胞的贴壁微载体悬浮培养方法 Download PDFInfo
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- CN107523534B CN107523534B CN201710851893.4A CN201710851893A CN107523534B CN 107523534 B CN107523534 B CN 107523534B CN 201710851893 A CN201710851893 A CN 201710851893A CN 107523534 B CN107523534 B CN 107523534B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
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- C12N2531/00—Microcarriers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
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CN201710851893.4A CN107523534B (zh) | 2017-09-20 | 2017-09-20 | 重组cho细胞的贴壁微载体悬浮培养方法 |
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CN107523534B true CN107523534B (zh) | 2020-10-02 |
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0245986A2 (en) * | 1986-04-28 | 1987-11-19 | Rohm And Haas Company | Immobilization of nonanchorage-dependent cells |
CN101096655A (zh) * | 2007-06-08 | 2008-01-02 | 冯来坤 | 微载体培养重组人组织型纤溶酶原激活剂tnk突变体的工艺 |
CN103289948A (zh) * | 2013-05-30 | 2013-09-11 | 冯玉萍 | 一种细胞培养用gf微载体在贴壁依赖型细胞培养中的应用 |
CN105143443A (zh) * | 2013-02-22 | 2015-12-09 | 建新公司 | 微载体灌注培养方法及其用途 |
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2017
- 2017-09-20 CN CN201710851893.4A patent/CN107523534B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0245986A2 (en) * | 1986-04-28 | 1987-11-19 | Rohm And Haas Company | Immobilization of nonanchorage-dependent cells |
CN101096655A (zh) * | 2007-06-08 | 2008-01-02 | 冯来坤 | 微载体培养重组人组织型纤溶酶原激活剂tnk突变体的工艺 |
CN105143443A (zh) * | 2013-02-22 | 2015-12-09 | 建新公司 | 微载体灌注培养方法及其用途 |
CN103289948A (zh) * | 2013-05-30 | 2013-09-11 | 冯玉萍 | 一种细胞培养用gf微载体在贴壁依赖型细胞培养中的应用 |
Non-Patent Citations (1)
Title |
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应用生物反应器高密度培养CHO细胞提高乙肝表面抗原表达量的研究;周艳红;《全国优秀硕士论文全文数据库》;20150301;第二部分摘要、"材料与方法" * |
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