CN107523505A - A kind of matrix for silk ball bacteria cultivation and preparation method thereof - Google Patents
A kind of matrix for silk ball bacteria cultivation and preparation method thereof Download PDFInfo
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- CN107523505A CN107523505A CN201710737285.0A CN201710737285A CN107523505A CN 107523505 A CN107523505 A CN 107523505A CN 201710737285 A CN201710737285 A CN 201710737285A CN 107523505 A CN107523505 A CN 107523505A
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- matrix
- silk ball
- sparassis crispa
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention discloses a kind of matrix for silk ball bacteria cultivation, including following composition：Water, starch, glucose, fish meal protein peptone, ammonium phosphate, magnesium sulfate, acrylic monomers, oxamides, to ethoxy benzamide, 4 hydroperoxyl endoxan, 1,1 cyclohexanediacetic acid monoamides, potassium peroxydisulfate, tricresyl phosphate (butoxyethyl group) ester.Matrix prepared by the present invention, formula material is simple and easy to get, can preferably preserve nutriment so that Sparassis crispa yield is higher.
Description
Technical field
The present invention relates to a kind of mushroom cultivation field, and in particular to a kind of matrix and its preparation side for silk ball bacteria cultivation
Method.
Background technology
Sparassis crispa, also known as silk ball mushroom, are non-pleat pore fungi mesh, silk ball Cordycepps, silk ball Pseudomonas, and its fructification meat is pure white fresh
It is tender, tasty, belong to one of rare wild edible fungus, the ground such as China Hebei, Jilin, Heilungkiang, Guangdong are distributed in, in day
Sheet, Europe, Oceania, North America are also distributed.Sparassis crispa has the activation immunocompetence of superelevation, containing substantial amounts of beta glucan,
Polyphenoils, there are good effects to dispelling the skin problem such as melanin deposition.In addition experiment proves that Sparassis crispa has to tumour
There is good inhibition, it is particularly evident in efficacy to breast cancer, stomach cancer, and also have to hypertension, diabetes and face well
Bed effect.
Because wild resource is rare, the cultivation of domestic Sparassis crispa is in developing stage, planting technique and technology need into
One step is perfect.In the prior art, it is most of that the culture to Sparassis crispa, the main carriers of soilless culture are realized by soilless culture
It is agar.But agar can not play the ability of locking nutriment very well as carrier so that Sparassis crispa growing way is bad.Separately
The outer report for also having some at present on Sparassis crispa liquid fermentation technology, but still there is limitations applied to cultivation, generally
Problem be present：1st, hypha biomass is low；2nd, culture medium raw material source inconvenience；3rd, complex operation, it is unfavorable for large-scale production.Cause
This, it is significant to develop a kind of raw material sources cultural method that is extensive, easy to operate, being adapted to large-scale production.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to provide a kind of matrix and the base for silk ball bacteria cultivation
The preparation method of matter.
To achieve the above object, the present invention provides a kind of matrix for silk ball bacteria cultivation, including following composition：Water, shallow lake
Powder, glucose, fish meal protein peptone, ammonium phosphate, magnesium sulfate, acrylic monomers, oxamides, to ethoxy benzamide, 4- peroxides
Hydroxyl endoxan, 1,1- cyclohexanediacetic acids monoamides, potassium peroxydisulfate, tricresyl phosphate (butoxyethyl group) ester.
Further, the present invention provides a kind of matrix for silk ball bacteria cultivation, including following parts by weight composition：
Water：100；
Starch：100；
Glucose：30；
Fish meal protein peptone：2；
Ammonium phosphate：2；
Magnesium sulfate：1；
Acrylic monomers：200；
Oxamides：20；
To ethoxy benzamide：10；
4- hydroperoxyl endoxan：10；
1,1- cyclohexanediacetic acid monoamides：2；
Potassium peroxydisulfate：1；
Tricresyl phosphate (butoxyethyl group) ester：10.
The present invention also provides the preparation method of the above-mentioned matrix for silk ball bacteria cultivation：
(1) acrylic monomers is weighed, pH value is adjusted by sodium hydroxide, is configured to the sodium acrylate solution that pH is 6-7；
(2) oxamides, formyl phenetidin, 4- hydroperoxyl endoxan are added to be well mixed, is warming up to 30 DEG C, instead
Answer 30 minutes；
(3) 1,1- cyclohexanediacetic acids monoamides, potassium peroxydisulfate are added, 60 DEG C of reaction 1-2 hours is continuously heating to, obtains
Polymer；
(4) resulting polymers are mixed with tricresyl phosphate (butoxyethyl group) ester, reacts 20 minutes, carried at 40 DEG C
Body；
(5) starch, glucose, fish meal protein peptone, ammonium phosphate and magnesium sulfate and water are mixed to get with nutriment
The aqueous solution, then the carrier obtained in upper step is dipped into the aqueous solution, obtains the matrix for cultivating Sparassis crispa.
Above each component is weighed according to formulation weight part.
Embodiment
Embodiment 1
A kind of matrix for silk ball bacteria cultivation, including following parts by weight composition：
Water：100；
Starch：100；
Glucose：30；
Fish meal protein peptone：2；
Ammonium phosphate：2；
Magnesium sulfate：1；
Acrylic monomers：200；
Oxamides：20；
To ethoxy benzamide：10；
4- hydroperoxyl endoxan：10；
1,1- cyclohexanediacetic acid monoamides：2；
Potassium peroxydisulfate：1；
Tricresyl phosphate (butoxyethyl group) ester：10.
The above-mentioned matrix for silk ball bacteria cultivation is prepared by the following method：
(1) acrylic monomers is weighed, pH value is adjusted by sodium hydroxide, is configured to the sodium acrylate solution that pH is 6-7；
(2) oxamides, formyl phenetidin, 4- hydroperoxyl endoxan are added to be well mixed, is warming up to 30 DEG C, instead
Answer 30 minutes；
(3) 1,1- cyclohexanediacetic acids monoamides, potassium peroxydisulfate are added, 60 DEG C of reaction 1-2 hours is continuously heating to, obtains
Polymer；
(4) resulting polymers are mixed with tricresyl phosphate (butoxyethyl group) ester, reacts 20 minutes, carried at 40 DEG C
Body；
(5) starch, glucose, fish meal protein peptone, ammonium phosphate and magnesium sulfate and water are mixed to get with nutriment
The aqueous solution, then the carrier obtained in upper step is dipped into the aqueous solution, obtains the matrix for cultivating Sparassis crispa.
Above each component is weighed according to formulation weight part.
Embodiment 2
By silk ball strain (preserving number：ACCC51488, purchased from Chinese agriculture microbial resources storehouse) it is inoculated in potato
Activate, 25 DEG C of cultivation temperature, cultivate 14 days on solid medium.It is seeded in the matrix of embodiment 1, fills by 10% inoculum concentration
Bottle, then carries out shaken cultivation 20 days under 25 DEG C, rotating speed 150rpm natural lightings, and 6000rpm is removed after centrifuging 10 minutes
Clear liquid, Sparassis crispa mycelium is obtained, it is 8.2g/L to measure its yield.
The general principle of the present invention, principal character and advantages of the present invention, the technology of the industry has been shown and described above
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the simply explanation described in above-described embodiment and specification is originally
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes
Change and improvement all fall within the protetion scope of the claimed invention.
Claims (4)
1. a kind of matrix for silk ball bacteria cultivation, including following composition：Water, starch, glucose, fish meal protein peptone, ammonium phosphate,
Magnesium sulfate, acrylic monomers, oxamides, to ethoxy benzamide, 4- hydroperoxyls endoxan, 1,1- cyclohexanediacetic acids
Monoamides, potassium peroxydisulfate, tricresyl phosphate (butoxyethyl group) ester.
2. a kind of matrix for silk ball bacteria cultivation, including following parts by weight composition：
Water：100；
Starch：100；
Glucose：30；
Fish meal protein peptone：2；
Ammonium phosphate：2；
Magnesium sulfate：1；
Acrylic monomers：200；
Oxamides：20；
To ethoxy benzamide：10；
4- hydroperoxyl endoxan：10；
1,1- cyclohexanediacetic acid monoamides：2；
Potassium peroxydisulfate：1；
Tricresyl phosphate (butoxyethyl group) ester：10.
3. the preparation method according to claim 1 or 2 for being used to cultivate the matrix of Sparassis crispa, including：
(1) acrylic monomers is weighed, pH value is adjusted by sodium hydroxide, is configured to the sodium acrylate solution that pH is 6-7；
(2) oxamides, formyl phenetidin, 4- hydroperoxyl endoxan are added to be well mixed, is warming up to 30 DEG C, reaction 30
Minute；
(3) 1,1- cyclohexanediacetic acids monoamides, potassium peroxydisulfate are added, 60 DEG C of reaction 1-2 hours is continuously heating to, is polymerize
Thing；
(4) resulting polymers are mixed with tricresyl phosphate (butoxyethyl group) ester, is reacted 20 minutes at 40 DEG C, obtain carrier；
(5) starch, glucose, fish meal protein peptone, ammonium phosphate and magnesium sulfate are mixed to get with the water-soluble of nutriment with water
Liquid, then the carrier obtained in upper step is dipped into the aqueous solution, obtains the matrix for cultivating Sparassis crispa.
4. the matrix for silk ball bacteria cultivation that method according to claim 3 is prepared, it is characterised in that connect Sparassis crispa
Kind is cultivated into the matrix, and it is 8.2g/L to obtain the mycelial yield of Sparassis crispa.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710737285.0A CN107523505A (en) | 2017-08-24 | 2017-08-24 | A kind of matrix for silk ball bacteria cultivation and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710737285.0A CN107523505A (en) | 2017-08-24 | 2017-08-24 | A kind of matrix for silk ball bacteria cultivation and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114540202A (en) * | 2020-07-10 | 2022-05-27 | 融和梦(福建)生物科技有限公司 | Preparation method of sparassis crispa dry powder, physiological function activator and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1713901B1 (en) * | 2004-02-11 | 2011-10-12 | Urea Casale S.A. | A culture medium for the production of filamentary fungi |
| CN102863271A (en) * | 2012-09-27 | 2013-01-09 | 中国科学院微生物研究所 | Synthetic medium of broad-leave Sparassis crispa |
| CN106365800A (en) * | 2016-08-29 | 2017-02-01 | 福建容益菌业科技研发有限公司 | Matrix for cultivating Sparassis crispa |
-
2017
- 2017-08-24 CN CN201710737285.0A patent/CN107523505A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1713901B1 (en) * | 2004-02-11 | 2011-10-12 | Urea Casale S.A. | A culture medium for the production of filamentary fungi |
| CN102863271A (en) * | 2012-09-27 | 2013-01-09 | 中国科学院微生物研究所 | Synthetic medium of broad-leave Sparassis crispa |
| CN106365800A (en) * | 2016-08-29 | 2017-02-01 | 福建容益菌业科技研发有限公司 | Matrix for cultivating Sparassis crispa |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114540202A (en) * | 2020-07-10 | 2022-05-27 | 融和梦(福建)生物科技有限公司 | Preparation method of sparassis crispa dry powder, physiological function activator and application thereof |
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