CN107494911A - The flower containing membranous milkvetch stem and leaf subtracts anti-feed, the method without using antibiotic cultivated animals - Google Patents
The flower containing membranous milkvetch stem and leaf subtracts anti-feed, the method without using antibiotic cultivated animals Download PDFInfo
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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Abstract
The invention discloses the method for subtracting anti-feed, edible animal being cultivated without using antibiotic containing the Radix Astragali or membranous milkvetch stem and leaf flower.The preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：(1) membranous milkvetch stem and leaf flower pretreatment；(2) mixed bacteria liquid is prepared；(3) ferment；(4) batch mixing.The method of present invention cultivation edible animal, without using antibiotic, the production performance and resistance against diseases that subtract anti-Feed Energy and improve edible animal spent containing the Radix Astragali or membranous milkvetch stem and leaf of use, the utilization rate of feed is improved, increases culture benefit.
Description
Technical field
The present invention relates to cultivation field, and in particular to subtracts anti-feed, without using antibiotic containing what the Radix Astragali or membranous milkvetch stem and leaf were spent
The method for cultivating edible animal.
Background technology
Consumption of resident meat eggs proportion in China's is increasing, and demand maintains sustained and rapid growth.Huge consumption needs
The amount of asking stimulates the development of aquaculture, but also brings challenge simultaneously.In order to improve production efficiency and maintain animal health, Ren Menkai
The antibiotic of in feed addition less than therapeutic dose begin as growth promoter (AGPs), however as the use of antibiotic,
It was found that the drug resistance increase of animal Endophytic bacteria, the drug resistant gene of bacterium, which can transmit, to be given people, and then endangers human health.Therefore Europe
Alliance announces to prohibit the use of antibiotic to come into effect in January, 2006, American plan was from 2014, with 3 as growth promoter
Year forbids adding preventive antibiotics in animal feeding-stuff, and the Ministry of Agriculture also begins to strict control antibiotic in feed
Use.But after AGPs disablings, the antibiotic usage amount for the treatment of greatly increases again.Therefore effective method is to find energy
Strengthen the material of animal natural defense function, to avoid a large amount of uses of antibiotic.
In order to ensure the sound development of animal husbandry, people are just sought for a kind of new technology, new product is substituted.Containing Huang
The anti-feed that subtracts of stilbene or membranous milkvetch stem and leaf flower is undoubtedly a kind of optimal selection as a kind of new environmental health type feed.Feed is former
For material by the fermentation process of microorganism, its nutriment inside has carried out a series of biochemical reaction, macromolecular therein
Organic matter is degraded to be digested to a certain extent the small-molecule substance of absorption, moreover it is possible to changes flavor and improves ingesting for feed
Rate, play the depth production and processing effect that feed manufacturing machine does not reach.
The Radix Astragali is the dry root of legume astragalus mongolicus or the pod membrane Radix Astragali, and main product is in the Inner Mongol, Shanxi, Gansu, black dragon
The ground such as river.Season in spring and autumn two excavates, and removes fibrous root and root head, dries, it is raw with or honey process use.First recorded in《Sheng Nong's herbal classic》It is conventional
Qi-tonifying drug.Sweet, the tepor of property.Return lung, the spleen channel.Active ingredient is polysaccharide, flavonoids, saponins, amino acid, trace element
Deng there is tonifying Qi and lifting yang, benefit defends solid table, sharp Chinese medical stone water detumescence, the effect of torr sore myogenic.Because the Radix Astragali is improving animal body
Immunity, antibacterial, antiviral, raising breeding performonce fo animals and improvement animal product quality etc. have good effect, then add
Above it has the characteristics of low-residual, have no drug resistance, and it is had a good application prospect in animal productiong.
The content of the invention
Problem to be solved by this invention is to provide the anti-feed that subtracts containing the Radix Astragali or membranous milkvetch stem and leaf flower and cultivates edible animal
Method, without using antibiotic, the production performance and resistance against diseases of edible animal can be improved.
The invention provides the method for subtracting anti-feed cultivation edible animal containing the Radix Astragali or membranous milkvetch stem and leaf flower, including use
Subtract anti-feed raising edible animal, preferably birds containing what the Radix Astragali or membranous milkvetch stem and leaf were spent.
The preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, it is small to be placed in 40-60 DEG C of drying box dry 18-24
When, crush as 60-100 mesh, obtain membranous milkvetch stem and leaf pollen；
(2) mixed bacteria liquid is prepared：By deionized water, Mixed Microbes, fermentation accelerant in mass ratio 100：(0.1-0.5)：
(0.5-1.5) is mixed, and with 60-100 revs/min of stirring 8-12 minute, obtains mixed bacteria liquid；
(3) ferment：Membranous milkvetch stem and leaf pollen 5-10 parts, corn 25-35 parts, wheat bran 10-20 parts, peanut meal are weighed by weight
25-35 parts, rice bran 10-20 parts, sucrose 1-5 parts, salt 0.01-0.2 parts, fermentation raw material, sprinkling mixing are obtained after well mixed
The dosage of bacterium solution, wherein mixed bacteria liquid is the 5-15% of fermentation raw material quality, then is sealed by fermentation 3-5 days at 25-35 DEG C, is sent out
Ferment product；
(4) batch mixing：By tunning, nutrition recuperating agent, acidulant in mass ratio 100：(5-10)：(0.01-0.1) is mixed
Close, then in 40-60 DEG C of drys 18-24 hour, obtain subtracting anti-feed containing what membranous milkvetch stem and leaf was spent.
It is preferred that the preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, it is small to be placed in 40-60 DEG C of drying box dry 18-24
When, crush as 60-100 mesh, obtain membranous milkvetch stem and leaf pollen；
(2) water extraction：By membranous milkvetch stem and leaf pollen and deionized water in mass ratio 1：(8-12) is mixed, in 90-100 DEG C of insulation
20-30 minutes, using 300 mesh filter-cloth filterings, first-time filtrate is obtained, going for 3-5 times of membranous milkvetch stem and leaf pollen weight is added in filter residue
Ionized water, 20-30 minutes are incubated at 90-100 DEG C, using 300 mesh filter-cloth filterings, obtain secondary filtrate, merge filtrate twice,
40-60 DEG C of be concentrated under reduced pressure into membranous milkvetch stem and leaf pollen weight 4-6 times, obtains Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：(0.1-0.5)：(0.5-
1.5) mix, with 60-100 revs/min of stirring 8-12 minute, obtain mixed bacteria liquid；
(4) ferment：Membranous milkvetch stem and leaf pollen 5-10 parts, corn 25-35 parts, wheat bran 10-20 parts, peanut meal are weighed by weight
25-35 parts, rice bran 10-20 parts, sucrose 1-5 parts, salt 0.01-0.2 parts, fermentation raw material, sprinkling mixing are obtained after well mixed
The dosage of bacterium solution, wherein mixed bacteria liquid is the 5-15% of fermentation raw material quality, then is sealed by fermentation 3-5 days at 25-35 DEG C, is sent out
Ferment product；
(5) batch mixing：By tunning, nutrition recuperating agent, acidulant in mass ratio 100：(5-10)：(0.01-0.1) is mixed
Close, then in 40-60 DEG C of drys 18-24 hour, obtain subtracting anti-feed containing what membranous milkvetch stem and leaf was spent.
It is preferred that the preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, it is small to be placed in 40-60 DEG C of drying box dry 18-24
When, crush as 60-100 mesh, obtain membranous milkvetch stem and leaf pollen；
(2) water extraction：By membranous milkvetch stem and leaf pollen and Chinese medical stone water in mass ratio 1：(8-12) is mixed, in 90-100 DEG C of insulation
20-30 minutes, using 300 mesh filter-cloth filterings, first-time filtrate is obtained, the wheat of 3-5 times of membranous milkvetch stem and leaf pollen weight is added in filter residue
Meal stone water, 20-30 minutes are incubated at 90-100 DEG C, using 300 mesh filter-cloth filterings, obtain secondary filtrate, merge filtrate twice,
40-60 DEG C of be concentrated under reduced pressure into membranous milkvetch stem and leaf pollen weight 4-6 times, obtains Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：(0.1-0.5)：(0.5-
1.5) mix, with 60-100 revs/min of stirring 8-12 minute, obtain mixed bacteria liquid；
(4) ferment：Membranous milkvetch stem and leaf pollen 5-10 parts, corn 25-35 parts, wheat bran 10-20 parts, peanut meal are weighed by weight
25-35 parts, rice bran 10-20 parts, sucrose 1-5 parts, salt 0.01-0.2 parts, fermentation raw material, sprinkling mixing are obtained after well mixed
The dosage of bacterium solution, wherein mixed bacteria liquid is the 5-15% of fermentation raw material quality, then is sealed by fermentation 3-5 days at 25-35 DEG C, is sent out
Ferment product；
(5) batch mixing：By tunning, nutrition recuperating agent, acidulant in mass ratio 100：(5-10)：(0.01-0.1) is mixed
Close, then in 40-60 DEG C of drys 18-24 hour, obtain subtracting anti-feed containing what membranous milkvetch stem and leaf was spent.
The preparation method for subtracting anti-feed containing the Radix Astragali comprises the following steps：
(1) Radix Astragali pre-processes：The root of the fresh Radix Astragali is gathered, is placed in 40-60 DEG C of drying box and dries 18-24 hours, is crushed
For 60-100 mesh, astragalus membranaceus powder is obtained；
(2) water extraction：By astragalus membranaceus powder and Chinese medical stone water in mass ratio 1：(8-12) is mixed, and 20-30 points are incubated at 90-100 DEG C
Clock, using 300 mesh filter-cloth filterings, first-time filtrate is obtained, the Chinese medical stone water of 3-5 times of astragalus membranaceus powder weight is added in filter residue, in 90-
100 DEG C of insulation 20-30 minutes, using 300 mesh filter-cloth filterings, secondary filtrate is obtained, merges filtrate twice, in 40-60 DEG C of decompression
4-6 times of astragalus membranaceus powder weight is concentrated into, obtains Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：(0.1-0.5)：(0.5-
1.5) mix, with 60-100 revs/min of stirring 8-12 minute, obtain mixed bacteria liquid；
(4) ferment：Astragalus membranaceus powder 5-10 parts, corn 25-35 parts, wheat bran 10-20 parts, peanut meal 25-35 are weighed by weight
Part, rice bran 10-20 parts, sucrose 1-5 parts, salt 0.01-0.2 parts, fermentation raw material is obtained after well mixed, sprays mixed bacteria liquid,
The dosage of wherein mixed bacteria liquid is the 5-15% of fermentation raw material quality, then is sealed by fermentation 3-5 days at 25-35 DEG C, obtains fermentation production
Thing；
(5) batch mixing：By tunning, nutrition recuperating agent, acidulant in mass ratio 100：(5-10)：(0.01-0.1) is mixed
Close, then in 40-60 DEG C of dry 18-24 hour, obtain subtracting anti-feed containing the Radix Astragali.
The Chinese medical stone water is prepared by following methods：By medical stone and deionized water in mass ratio 1：(5-10) is mixed
Close, soak 40-60 minutes at 45-55 DEG C, with 4000-8000 revs/min of centrifugation 4-8 minute, take supernatant, obtain Chinese medical stone water.
The nutrition recuperating agent includes the raw material of following weight parts：Whey powder 20-30 parts, Gluten 25-35 parts, corn flour
25-35 parts, glucose 5-10 parts, calcium monohydrogen phosphate 4-8 parts, soybean oil 1-3 parts.
The nutrition recuperating agent is prepared by following methods：By whey powder, Gluten, corn flour, glucose, phosphoric acid hydrogen
Calcium, soybean oil are well mixed, and obtain nutrition recuperating agent.
The Mixed Microbes are bacillus subtilis, candida utili, lactobacillus acidophilus (1-3) in mass ratio：(0.1-1)
The mixture of (1-2).
The acidulant is one kind in lactic acid, citric acid, tartaric acid, fumaric acid, sorbic acid, chlorogenic acid.
It is preferred that the acidulant is chlorogenic acid.
The fermentation accelerant is polyglyceryl fatty acid ester and/or Tea Polyphenols.
It is preferred that the fermentation accelerant is the mixture of polyglyceryl fatty acid ester and Tea Polyphenols, the polyglycereol fat
The mass ratio of acid esters and Tea Polyphenols is (2-5)：1.
In the present invention, the edible animal includes birds, domestic animals, fishing class.
The birds include family chicken (Callus domesticus) cock, turkey (Meleagris gllaopavo), treasure
Galeeny (Numida meleagris), dove (Columba livia), duck (Anas Platyrhynchus
Var.domestica), goose (Anas cygnoides).
The domestic animal includes pig, ox, sheep, horse, camel, rabbit, dog.
The fishing class includes fish, shrimp, crab, soft-shelled turtle, shellfish, Echinodermata, software class, coelenteron class, amphibian animal, extra large beast-like animals.
The method for subtracting anti-feed cultivation edible animal that the present invention is spent containing the Radix Astragali or membranous milkvetch stem and leaf, without using antibiotic,
The production performance and resistance against diseases that subtract anti-Feed Energy and improve birds spent containing the Radix Astragali or membranous milkvetch stem and leaf used, improve the profit of feed
With rate, increase culture benefit.
Embodiment
The present invention is expanded on further with reference to specific embodiment.It should be appreciated, however, that these embodiments are only used for
It is bright the present invention and be not meant to limit the scope of the invention.The test method of unreceipted actual conditions in the following example, generally
According to normal condition, or according to the condition proposed by manufacturer.
Bacillus subtilis, the bacillus subtilis provided using Chinese industrial Microbiological Culture Collection administrative center, bacterium
Strain deposit number：CICC 10002, viable bacteria concentration 1011CFU/g。
Candida utili, the candida utili provided using Chinese industrial Microbiological Culture Collection administrative center, bacterium
Strain deposit number：CICC 1801, viable bacteria concentration 1011CFU/g。
Lactobacillus acidophilus, the lactobacillus acidophilus provided using Chinese industrial Microbiological Culture Collection administrative center, bacterial strain are protected
Hide numbering：CICC 20250, viable bacteria concentration 1011CFU/g。
Membranous milkvetch stem and leaf is spent, and is spent using the cauline leaf of Inner Mongolia Astragalis, and the Shangdu County for picking up from Bei Tian protozoas Science and Technology Ltd. is yellow
Stilbene planting base.
The Radix Astragali, using the root of Inner Mongolia Astragalis, pick up from the Shangdu County astragalus cultivation base of Bei Tian protozoas Science and Technology Ltd..
Medical stone, the medical stone provided using Wen County Hong Yu activated carbons factory, the mesh of particle diameter 100.
Corn, the iblet produced using the edge agricultural science and technology Co., Ltd in Linyi City autumn.
Wheat bran, the wheat bran produced using the Li Wan crop cultivation specialities cooperative society of Wuxiang County three.
Peanut meal, the peanut meal produced using Lingshou County Chuan Bei agricultural science and technologys Co., Ltd.
Rice bran, the rice bran produced using the phoenix grain and oil Co., Ltd of Sichuan ten thousand.
Salt, i.e. sodium chloride, No. CAS：7647-14-5, the refined sea salt produced using Zhong Yan Shanghai City Salt Company.
Whey powder, the low albumen whey powder produced using Shandong Ming Zheng bio tech ltd, lactose content 65%-
75%, crude protein content 12%.
Gluten, the wheat gluten flour produced using Zhengzhou Jiang great bio tech ltd.
Corn flour, using the expanded corn powder of the emerging Chemical Co., Ltd.'s production in Qinyang City.
Sucrose, No. CAS：57-50-1.
Glucose, No. CAS：492-62-6.
Calcium monohydrogen phosphate, No. CAS：7789-77-7.
Soybean oil, using golden dragonfish one-level soybean oil.
Lactic acid, No. CAS：50-21-5.
Citric acid, No. CAS：77-92-9.
Tartaric acid, No. CAS：526-83-0.
Fumaric acid, No. CAS：110-17-8.
Sorbic acid, No. CAS：110-44-1.
Chlorogenic acid, No. CAS：327-97-9.
Polyglyceryl fatty acid ester, using the food-grade polyglycereol fat of Shandong Ke Nuosite bio tech ltd production
Acid esters.
Tea Polyphenols, 84650-60-2, the food-grade Tea Polyphenols produced using one hundred emerging bio tech ltd of Wuhan.
Broiler chicken, using 1 age in days Ai Wei mattress chick.
Performance test：In the 1st age in days and the 42nd age in days, each embodiment randomly selects 40 broiler chicken, on an empty stomach 12
Broiler chicken after hour is weighed, and record original body mass, Slaughter weight, the daily feeding of each embodiment is recorded during experiment
Amount, calculate feedstuff-meat ratio.
Feedstuff-meat ratio=total feed intake/total augment weight
Development of Immune Organs situation determines：The bursa of farbricius is the distinctive central immune system of birds, be immune cell growth and
The position of differentiation.Spleen is important peripheral lymphoid organs, can produce substantial amounts of lymphocyte, participate in whole body cellular immunity and
Humoral immunity.The performance that immune organ weight increase strengthens for immunologic function, and it is then immunosupress that immune organ weight, which reduces,
It is caused.
Assay method：Before the 42nd age in days early morning fed, each embodiment randomly selects 5 broiler chicken, after weighing, using mouth
Chamber depletion method is slaughtered, and takes spleen and the bursa of farbricius, rejects fat, fresh weight, Computation immunity shoot formation are claimed after cleaning.
Empty stomach live-weight (Kg) before Immune Organs Index=immune organ weight (g)/government official
Embodiment 1
Subtract the method for anti-feed cultivation edible animal containing what the Radix Astragali or membranous milkvetch stem and leaf were spent, including use and contain the Radix Astragali or the Radix Astragali
Cauline leaf flower subtracts anti-feed raising birds.
1 Day-old Broiler Chickens are chosen, whole process is fed subtracts anti-feed containing what membranous milkvetch stem and leaf was spent.Sub-cage rearing, free choice feeding and drinking-water,
Routine immunization.
The preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 50 DEG C of drying boxes and dries 20 hours, powder
Broken is 80 mesh, obtains membranous milkvetch stem and leaf pollen；
(2) mixed bacteria liquid is prepared：By deionized water, Mixed Microbes, fermentation accelerant in mass ratio 100：0.4：1 mixing, with
80 revs/min are stirred 10 minutes, obtain mixed bacteria liquid；
(3) ferment：8 parts of membranous milkvetch stem and leaf pollen, 30 parts of corn, 15 parts of wheat bran, 30 parts of peanut meal, rice bran are weighed by weight
15 parts, 2 parts of sucrose, 0.05 part of salt, fermentation raw material is obtained after well mixed, sprays the use of mixed bacteria liquid, wherein mixed bacteria liquid
Amount is the 10% of fermentation raw material quality, then is sealed by fermentation 4 days at 30 DEG C, obtains tunning；
(4) batch mixing：By tunning, nutrition recuperating agent, acidulant in mass ratio 100：8：0.05 mixing, then it is dry at 50 DEG C
Dry 20 hours, obtain the flower containing membranous milkvetch stem and leaf subtracted anti-feed.
The nutrition recuperating agent includes the raw material of following weight parts：25 parts of whey powder, 30 parts of Gluten, 30 parts of corn flour,
7 parts of glucose, 6 parts of calcium monohydrogen phosphate, 2 parts of soybean oil.The nutrition recuperating agent is prepared by following methods：By whey powder, paddy
Protein powder, corn flour, glucose, calcium monohydrogen phosphate, soybean oil are well mixed, and obtain nutrition recuperating agent.
The Mixed Microbes are bacillus subtilis, candida utili, lactobacillus acidophilus in mass ratio 2：0.5：1.5 it is mixed
Compound.
The acidulant is lactic acid.
The fermentation accelerant is polyglyceryl fatty acid ester.
Embodiment 2
Subtract the method for anti-feed cultivation edible animal containing what the Radix Astragali or membranous milkvetch stem and leaf were spent, including use and contain the Radix Astragali or the Radix Astragali
Cauline leaf flower subtracts anti-feed raising birds.
1 Day-old Broiler Chickens are chosen, whole process is fed subtracts anti-feed containing what membranous milkvetch stem and leaf was spent.Sub-cage rearing, free choice feeding and drinking-water,
Routine immunization.
The preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 50 DEG C of drying boxes and dries 20 hours, powder
Broken is 80 mesh, obtains membranous milkvetch stem and leaf pollen；
(2) water extraction：By membranous milkvetch stem and leaf pollen and deionized water in mass ratio 1：10 mixing, 25 minutes are incubated at 95 DEG C, is adopted
With 300 mesh filter-cloth filterings, first-time filtrate is obtained, the deionized water of 4 times of membranous milkvetch stem and leaf pollen weight is added in filter residue in 95 DEG C of guarantors
Temperature 25 minutes, using 300 mesh filter-cloth filterings, secondary filtrate is obtained, merges filtrate twice, be 50 DEG C in pressure 0.06MPa, temperature
Condition vacuum-concentrcted to 5 times of membranous milkvetch stem and leaf pollen weight, obtain Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：0.4：1 mixing, with 80
Rev/min stirring 10 minutes, obtain mixed bacteria liquid；
(4) ferment：8 parts of membranous milkvetch stem and leaf pollen, 30 parts of corn, 15 parts of wheat bran, 30 parts of peanut meal, rice bran are weighed by weight
15 parts, 2 parts of sucrose, 0.05 part of salt, fermentation raw material is obtained after well mixed, sprays the use of mixed bacteria liquid, wherein mixed bacteria liquid
Amount is the 10% of fermentation raw material quality, then is sealed by fermentation 4 days at 30 DEG C, obtains tunning；
(5) by tunning, nutrition recuperating agent, acidulant in mass ratio 100：8：0.05 mixing, then dry 20 at 50 DEG C
Hour, obtain the flower containing membranous milkvetch stem and leaf subtracts anti-feed.
The nutrition recuperating agent includes the raw material of following weight parts：25 parts of whey powder, 30 parts of Gluten, 30 parts of corn flour,
7 parts of glucose, 6 parts of calcium monohydrogen phosphate, 2 parts of soybean oil.The nutrition recuperating agent is prepared by following methods：By whey powder, paddy
Protein powder, corn flour, glucose, calcium monohydrogen phosphate, soybean oil are well mixed, and obtain nutrition recuperating agent.
The Mixed Microbes are bacillus subtilis, candida utili, lactobacillus acidophilus in mass ratio 2：0.5：1.5 it is mixed
Compound.
The acidulant is lactic acid.
The fermentation accelerant is polyglyceryl fatty acid ester.
Embodiment 3
Subtract the method for anti-feed cultivation edible animal containing what the Radix Astragali or membranous milkvetch stem and leaf were spent, including use and contain the Radix Astragali or the Radix Astragali
Cauline leaf flower subtracts anti-feed raising birds.
1 Day-old Broiler Chickens are chosen, whole process is fed subtracts anti-feed containing what membranous milkvetch stem and leaf was spent.Sub-cage rearing, free choice feeding and drinking-water,
Routine immunization.
The preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 50 DEG C of drying boxes and dries 20 hours, powder
Broken is 80 mesh, obtains membranous milkvetch stem and leaf pollen；
(2) water extraction：By membranous milkvetch stem and leaf pollen and Chinese medical stone water in mass ratio 1：10 mixing, 25 minutes are incubated at 95 DEG C, is adopted
With 300 mesh filter-cloth filterings, first-time filtrate is obtained, the Chinese medical stone water of 4 times of membranous milkvetch stem and leaf pollen weight is added in filter residue, in 95 DEG C of guarantors
Temperature 25 minutes, using 300 mesh filter-cloth filterings, secondary filtrate is obtained, merges filtrate twice, be 50 DEG C in pressure 0.06MPa, temperature
Condition vacuum-concentrcted to 5 times of membranous milkvetch stem and leaf pollen weight, obtain Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：0.4：1 mixing, with 80
Rev/min stirring 10 minutes, obtain mixed bacteria liquid；
(4) ferment：8 parts of membranous milkvetch stem and leaf pollen, 30 parts of corn, 15 parts of wheat bran, 30 parts of peanut meal, rice bran are weighed by weight
15 parts, 2 parts of sucrose, 0.05 part of salt, fermentation raw material is obtained after well mixed, sprays the use of mixed bacteria liquid, wherein mixed bacteria liquid
Amount is the 10% of fermentation raw material quality, then is sealed by fermentation 4 days at 30 DEG C, obtains tunning；
(5) by tunning, nutrition recuperating agent, acidulant in mass ratio 100：8：0.05 mixing, then dry 20 at 50 DEG C
Hour, obtain the flower containing membranous milkvetch stem and leaf subtracts anti-feed.
The Chinese medical stone water is prepared by following methods：By medical stone and deionized water in mass ratio 1：8 mixing, 50
DEG C immersion 50 minutes, with 6000 revs/min centrifuge 5 minutes, take supernatant, obtain Chinese medical stone water.
The nutrition recuperating agent includes the raw material of following weight parts：25 parts of whey powder, 30 parts of Gluten, 30 parts of corn flour,
7 parts of glucose, 6 parts of calcium monohydrogen phosphate, 2 parts of soybean oil.The nutrition recuperating agent is prepared by following methods：By whey powder, paddy
Protein powder, corn flour, glucose, calcium monohydrogen phosphate, soybean oil are well mixed, and obtain nutrition recuperating agent.
The Mixed Microbes are bacillus subtilis, candida utili, lactobacillus acidophilus in mass ratio 2：0.5：1.5 it is mixed
Compound.
The acidulant is lactic acid.
The fermentation accelerant is polyglyceryl fatty acid ester.
Embodiment 4
Subtract the method for anti-feed cultivation edible animal containing what the Radix Astragali or membranous milkvetch stem and leaf were spent, including use and contain the Radix Astragali or the Radix Astragali
Cauline leaf flower subtracts anti-feed raising birds.
1 Day-old Broiler Chickens are chosen, whole process is fed subtracts anti-feed containing what membranous milkvetch stem and leaf was spent.Sub-cage rearing, free choice feeding and drinking-water,
Routine immunization.
The preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 50 DEG C of drying boxes and dries 20 hours, powder
Broken is 80 mesh, obtains membranous milkvetch stem and leaf pollen；
(2) water extraction：By membranous milkvetch stem and leaf pollen and Chinese medical stone water in mass ratio 1：10 mixing, 25 minutes are incubated at 95 DEG C, is adopted
With 300 mesh filter-cloth filterings, first-time filtrate is obtained, the Chinese medical stone water of 4 times of membranous milkvetch stem and leaf pollen weight is added in filter residue, in 95 DEG C of guarantors
Temperature 25 minutes, using 300 mesh filter-cloth filterings, secondary filtrate is obtained, merges filtrate twice, be 50 DEG C in pressure 0.06MPa, temperature
Condition vacuum-concentrcted to 5 times of membranous milkvetch stem and leaf pollen weight, obtain Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：0.4：1 mixing, with 80
Rev/min stirring 10 minutes, obtain mixed bacteria liquid；
(4) ferment：8 parts of membranous milkvetch stem and leaf pollen, 30 parts of corn, 15 parts of wheat bran, 30 parts of peanut meal, rice bran are weighed by weight
15 parts, 2 parts of sucrose, 0.05 part of salt, fermentation raw material is obtained after well mixed, sprays the use of mixed bacteria liquid, wherein mixed bacteria liquid
Amount is the 10% of fermentation raw material quality, then is sealed by fermentation 4 days at 30 DEG C, obtains tunning；
(5) by tunning, nutrition recuperating agent, acidulant in mass ratio 100：8：0.05 mixing, then dry 20 at 50 DEG C
Hour, obtain the flower containing membranous milkvetch stem and leaf subtracts anti-feed.
The Chinese medical stone water is prepared by following methods：By medical stone and deionized water in mass ratio 1：8 mixing, 50
DEG C immersion 50 minutes, with 6000 revs/min centrifuge 5 minutes, take supernatant, obtain Chinese medical stone water.
The nutrition recuperating agent includes the raw material of following weight parts：25 parts of whey powder, 30 parts of Gluten, 30 parts of corn flour,
7 parts of glucose, 6 parts of calcium monohydrogen phosphate, 2 parts of soybean oil.The nutrition recuperating agent is prepared by following methods：By whey powder, paddy
Protein powder, corn flour, glucose, calcium monohydrogen phosphate, soybean oil are well mixed, and obtain nutrition recuperating agent.
The Mixed Microbes are bacillus subtilis, candida utili, lactobacillus acidophilus in mass ratio 2：0.5：1.5 it is mixed
Compound.
The acidulant is citric acid.
The fermentation accelerant is polyglyceryl fatty acid ester.
Embodiment 5
Subtract the method for anti-feed cultivation edible animal containing what the Radix Astragali or membranous milkvetch stem and leaf were spent, including use and contain the Radix Astragali or the Radix Astragali
Cauline leaf flower subtracts anti-feed raising birds.
1 Day-old Broiler Chickens are chosen, whole process is fed subtracts anti-feed containing what membranous milkvetch stem and leaf was spent.Sub-cage rearing, free choice feeding and drinking-water,
Routine immunization.
The preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 50 DEG C of drying boxes and dries 20 hours, powder
Broken is 80 mesh, obtains membranous milkvetch stem and leaf pollen；
(2) water extraction：By membranous milkvetch stem and leaf pollen and Chinese medical stone water in mass ratio 1：10 mixing, 25 minutes are incubated at 95 DEG C, is adopted
With 300 mesh filter-cloth filterings, first-time filtrate is obtained, the Chinese medical stone water of 4 times of membranous milkvetch stem and leaf pollen weight is added in filter residue, in 95 DEG C of guarantors
Temperature 25 minutes, using 300 mesh filter-cloth filterings, secondary filtrate is obtained, merges filtrate twice, be 50 DEG C in pressure 0.06MPa, temperature
Condition vacuum-concentrcted to 5 times of membranous milkvetch stem and leaf pollen weight, obtain Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：0.4：1 mixing, with 80
Rev/min stirring 10 minutes, obtain mixed bacteria liquid；
(4) ferment：8 parts of membranous milkvetch stem and leaf pollen, 30 parts of corn, 15 parts of wheat bran, 30 parts of peanut meal, rice bran are weighed by weight
15 parts, 2 parts of sucrose, 0.05 part of salt, fermentation raw material is obtained after well mixed, sprays the use of mixed bacteria liquid, wherein mixed bacteria liquid
Amount is the 10% of fermentation raw material quality, then is sealed by fermentation 4 days at 30 DEG C, obtains tunning；
(5) by tunning, nutrition recuperating agent, acidulant in mass ratio 100：8：0.05 mixing, then dry 20 at 50 DEG C
Hour, obtain the flower containing membranous milkvetch stem and leaf subtracts anti-feed.
The Chinese medical stone water is prepared by following methods：By medical stone and deionized water in mass ratio 1：8 mixing, 50
DEG C immersion 50 minutes, with 6000 revs/min centrifuge 5 minutes, take supernatant, obtain Chinese medical stone water.
The nutrition recuperating agent includes the raw material of following weight parts：25 parts of whey powder, 30 parts of Gluten, 30 parts of corn flour,
7 parts of glucose, 6 parts of calcium monohydrogen phosphate, 2 parts of soybean oil.The nutrition recuperating agent is prepared by following methods：By whey powder, paddy
Protein powder, corn flour, glucose, calcium monohydrogen phosphate, soybean oil are well mixed, and obtain nutrition recuperating agent.
The Mixed Microbes are bacillus subtilis, candida utili, lactobacillus acidophilus in mass ratio 2：0.5：1.5 it is mixed
Compound.
The acidulant is tartaric acid.
The fermentation accelerant is polyglyceryl fatty acid ester.
Embodiment 6
Subtract the method for anti-feed cultivation edible animal containing what the Radix Astragali or membranous milkvetch stem and leaf were spent, including use and contain the Radix Astragali or the Radix Astragali
Cauline leaf flower subtracts anti-feed raising birds.
1 Day-old Broiler Chickens are chosen, whole process is fed subtracts anti-feed containing what membranous milkvetch stem and leaf was spent.Sub-cage rearing, free choice feeding and drinking-water,
Routine immunization.
The preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 50 DEG C of drying boxes and dries 20 hours, powder
Broken is 80 mesh, obtains membranous milkvetch stem and leaf pollen；
(2) water extraction：By membranous milkvetch stem and leaf pollen and Chinese medical stone water in mass ratio 1：10 mixing, 25 minutes are incubated at 95 DEG C, is adopted
With 300 mesh filter-cloth filterings, first-time filtrate is obtained, the Chinese medical stone water of 4 times of membranous milkvetch stem and leaf pollen weight is added in filter residue, in 95 DEG C of guarantors
Temperature 25 minutes, using 300 mesh filter-cloth filterings, secondary filtrate is obtained, merges filtrate twice, be 50 DEG C in pressure 0.06MPa, temperature
Condition vacuum-concentrcted to 5 times of membranous milkvetch stem and leaf pollen weight, obtain Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：0.4：1 mixing, with 80
Rev/min stirring 10 minutes, obtain mixed bacteria liquid；
(4) ferment：8 parts of membranous milkvetch stem and leaf pollen, 30 parts of corn, 15 parts of wheat bran, 30 parts of peanut meal, rice bran are weighed by weight
15 parts, 2 parts of sucrose, 0.05 part of salt, fermentation raw material is obtained after well mixed, sprays the use of mixed bacteria liquid, wherein mixed bacteria liquid
Amount is the 10% of fermentation raw material quality, then is sealed by fermentation 4 days at 30 DEG C, obtains tunning；
(5) by tunning, nutrition recuperating agent, acidulant in mass ratio 100：8：0.05 mixing, then dry 20 at 50 DEG C
Hour, obtain the flower containing membranous milkvetch stem and leaf subtracts anti-feed.
The Chinese medical stone water is prepared by following methods：By medical stone and deionized water in mass ratio 1：8 mixing, 50
DEG C immersion 50 minutes, with 6000 revs/min centrifuge 5 minutes, take supernatant, obtain Chinese medical stone water.
The nutrition recuperating agent includes the raw material of following weight parts：25 parts of whey powder, 30 parts of Gluten, 30 parts of corn flour,
7 parts of glucose, 6 parts of calcium monohydrogen phosphate, 2 parts of soybean oil.The nutrition recuperating agent is prepared by following methods：By whey powder, paddy
Protein powder, corn flour, glucose, calcium monohydrogen phosphate, soybean oil are well mixed, and obtain nutrition recuperating agent.
The Mixed Microbes are bacillus subtilis, candida utili, lactobacillus acidophilus in mass ratio 2：0.5：1.5 it is mixed
Compound.
The acidulant is fumaric acid.
The fermentation accelerant is polyglyceryl fatty acid ester.
Embodiment 7
Subtract the method for anti-feed cultivation edible animal containing what the Radix Astragali or membranous milkvetch stem and leaf were spent, including use and contain the Radix Astragali or the Radix Astragali
Cauline leaf flower subtracts anti-feed raising birds.
1 Day-old Broiler Chickens are chosen, whole process is fed subtracts anti-feed containing what membranous milkvetch stem and leaf was spent.Sub-cage rearing, free choice feeding and drinking-water,
Routine immunization.
The preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 50 DEG C of drying boxes and dries 20 hours, powder
Broken is 80 mesh, obtains membranous milkvetch stem and leaf pollen；
(2) water extraction：By membranous milkvetch stem and leaf pollen and Chinese medical stone water in mass ratio 1：10 mixing, 25 minutes are incubated at 95 DEG C, is adopted
With 300 mesh filter-cloth filterings, first-time filtrate is obtained, the Chinese medical stone water of 4 times of membranous milkvetch stem and leaf pollen weight is added in filter residue, in 95 DEG C of guarantors
Temperature 25 minutes, using 300 mesh filter-cloth filterings, secondary filtrate is obtained, merges filtrate twice, be 50 DEG C in pressure 0.06MPa, temperature
Condition vacuum-concentrcted to 5 times of membranous milkvetch stem and leaf pollen weight, obtain Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：0.4：1 mixing, with 80
Rev/min stirring 10 minutes, obtain mixed bacteria liquid；
(4) ferment：8 parts of membranous milkvetch stem and leaf pollen, 30 parts of corn, 15 parts of wheat bran, 30 parts of peanut meal, rice bran are weighed by weight
15 parts, 2 parts of sucrose, 0.05 part of salt, fermentation raw material is obtained after well mixed, sprays the use of mixed bacteria liquid, wherein mixed bacteria liquid
Amount is the 10% of fermentation raw material quality, then is sealed by fermentation 4 days at 30 DEG C, obtains tunning；
(5) by tunning, nutrition recuperating agent, acidulant in mass ratio 100：8：0.05 mixing, then dry 20 at 50 DEG C
Hour, obtain the flower containing membranous milkvetch stem and leaf subtracts anti-feed.
The Chinese medical stone water is prepared by following methods：By medical stone and deionized water in mass ratio 1：8 mixing, 50
DEG C immersion 50 minutes, with 6000 revs/min centrifuge 5 minutes, take supernatant, obtain Chinese medical stone water.
The nutrition recuperating agent includes the raw material of following weight parts：25 parts of whey powder, 30 parts of Gluten, 30 parts of corn flour,
7 parts of glucose, 6 parts of calcium monohydrogen phosphate, 2 parts of soybean oil.The nutrition recuperating agent is prepared by following methods：By whey powder, paddy
Protein powder, corn flour, glucose, calcium monohydrogen phosphate, soybean oil are well mixed, and obtain nutrition recuperating agent.
The Mixed Microbes are bacillus subtilis, candida utili, lactobacillus acidophilus in mass ratio 2：0.5：1.5 it is mixed
Compound.
The acidulant is sorbic acid.
The fermentation accelerant is polyglyceryl fatty acid ester.
Embodiment 8
Subtract the method for anti-feed cultivation edible animal containing what the Radix Astragali or membranous milkvetch stem and leaf were spent, including use and contain the Radix Astragali or the Radix Astragali
Cauline leaf flower subtracts anti-feed raising birds.
1 Day-old Broiler Chickens are chosen, whole process is fed subtracts anti-feed containing what membranous milkvetch stem and leaf was spent.Sub-cage rearing, free choice feeding and drinking-water,
Routine immunization.
The preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 50 DEG C of drying boxes and dries 20 hours, powder
Broken is 80 mesh, obtains membranous milkvetch stem and leaf pollen；
(2) water extraction：By membranous milkvetch stem and leaf pollen and Chinese medical stone water in mass ratio 1：10 mixing, 25 minutes are incubated at 95 DEG C, is adopted
With 300 mesh filter-cloth filterings, first-time filtrate is obtained, the Chinese medical stone water of 4 times of membranous milkvetch stem and leaf pollen weight is added in filter residue, in 95 DEG C of guarantors
Temperature 25 minutes, using 300 mesh filter-cloth filterings, secondary filtrate is obtained, merges filtrate twice, be 50 DEG C in pressure 0.06MPa, temperature
Condition vacuum-concentrcted to 5 times of membranous milkvetch stem and leaf pollen weight, obtain Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：0.4：1 mixing, with 80
Rev/min stirring 10 minutes, obtain mixed bacteria liquid；
(4) ferment：8 parts of membranous milkvetch stem and leaf pollen, 30 parts of corn, 15 parts of wheat bran, 30 parts of peanut meal, rice bran are weighed by weight
15 parts, 2 parts of sucrose, 0.05 part of salt, fermentation raw material is obtained after well mixed, sprays the use of mixed bacteria liquid, wherein mixed bacteria liquid
Amount is the 10% of fermentation raw material quality, then is sealed by fermentation 4 days at 30 DEG C, obtains tunning；
(5) by tunning, nutrition recuperating agent, acidulant in mass ratio 100：8：0.05 mixing, then dry 20 at 50 DEG C
Hour, obtain the flower containing membranous milkvetch stem and leaf subtracts anti-feed.
The Chinese medical stone water is prepared by following methods：By medical stone and deionized water in mass ratio 1：8 mixing, 50
DEG C immersion 50 minutes, with 6000 revs/min centrifuge 5 minutes, take supernatant, obtain Chinese medical stone water.
The nutrition recuperating agent includes the raw material of following weight parts：25 parts of whey powder, 30 parts of Gluten, 30 parts of corn flour,
7 parts of glucose, 6 parts of calcium monohydrogen phosphate, 2 parts of soybean oil.The nutrition recuperating agent is prepared by following methods：By whey powder, paddy
Protein powder, corn flour, glucose, calcium monohydrogen phosphate, soybean oil are well mixed, and obtain nutrition recuperating agent.
The Mixed Microbes are bacillus subtilis, candida utili, lactobacillus acidophilus in mass ratio 2：0.5：1.5 it is mixed
Compound.
The acidulant is chlorogenic acid.
The fermentation accelerant is polyglyceryl fatty acid ester.
Embodiment 9
Subtract the method for anti-feed cultivation edible animal containing what the Radix Astragali or membranous milkvetch stem and leaf were spent, including use and contain the Radix Astragali or the Radix Astragali
Cauline leaf flower subtracts anti-feed raising birds.
1 Day-old Broiler Chickens are chosen, whole process is fed subtracts anti-feed containing what membranous milkvetch stem and leaf was spent.Sub-cage rearing, free choice feeding and drinking-water,
Routine immunization.
The preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 50 DEG C of drying boxes and dries 20 hours, powder
Broken is 80 mesh, obtains membranous milkvetch stem and leaf pollen；
(2) water extraction：By membranous milkvetch stem and leaf pollen and Chinese medical stone water in mass ratio 1：10 mixing, 25 minutes are incubated at 95 DEG C, is adopted
With 300 mesh filter-cloth filterings, first-time filtrate is obtained, the Chinese medical stone water of 4 times of membranous milkvetch stem and leaf pollen weight is added in filter residue, in 95 DEG C of guarantors
Temperature 25 minutes, using 300 mesh filter-cloth filterings, secondary filtrate is obtained, merges filtrate twice, be 50 DEG C in pressure 0.06MPa, temperature
Condition vacuum-concentrcted to 5 times of membranous milkvetch stem and leaf pollen weight, obtain Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：0.4：1 mixing, with 80
Rev/min stirring 10 minutes, obtain mixed bacteria liquid；
(4) ferment：8 parts of membranous milkvetch stem and leaf pollen, 30 parts of corn, 15 parts of wheat bran, 30 parts of peanut meal, rice bran are weighed by weight
15 parts, 2 parts of sucrose, 0.05 part of salt, fermentation raw material is obtained after well mixed, sprays the use of mixed bacteria liquid, wherein mixed bacteria liquid
Amount is the 10% of fermentation raw material quality, then is sealed by fermentation 4 days at 30 DEG C, obtains tunning；
(5) by tunning, nutrition recuperating agent, acidulant in mass ratio 100：8：0.05 mixing, then dry 20 at 50 DEG C
Hour, obtain the flower containing membranous milkvetch stem and leaf subtracts anti-feed.
The Chinese medical stone water is prepared by following methods：By medical stone and deionized water in mass ratio 1：8 mixing, 50
DEG C immersion 50 minutes, with 6000 revs/min centrifuge 5 minutes, take supernatant, obtain Chinese medical stone water.
The nutrition recuperating agent includes the raw material of following weight parts：25 parts of whey powder, 30 parts of Gluten, 30 parts of corn flour,
7 parts of glucose, 6 parts of calcium monohydrogen phosphate, 2 parts of soybean oil.The nutrition recuperating agent is prepared by following methods：By whey powder, paddy
Protein powder, corn flour, glucose, calcium monohydrogen phosphate, soybean oil are well mixed, and obtain nutrition recuperating agent.
The Mixed Microbes are bacillus subtilis, candida utili, lactobacillus acidophilus in mass ratio 2：0.5：1.5 it is mixed
Compound.
The acidulant is chlorogenic acid.
The fermentation accelerant is Tea Polyphenols.
Meat chicken production performance test result：Average daily gain 102.75g, average daily gain 57.34g, feedstuff-meat ratio 1.79；
The Development of Immune Organs situation test result of broiler chicken：Index and spleen index 1.81, bursal index 1.29.
Embodiment 10
Subtract the method for anti-feed cultivation edible animal containing what the Radix Astragali or membranous milkvetch stem and leaf were spent, including use and contain the Radix Astragali or the Radix Astragali
Cauline leaf flower subtracts anti-feed raising birds.
1 Day-old Broiler Chickens are chosen, whole process is fed subtracts anti-feed containing what membranous milkvetch stem and leaf was spent.Sub-cage rearing, free choice feeding and drinking-water,
Routine immunization.
The preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 50 DEG C of drying boxes and dries 20 hours, powder
Broken is 80 mesh, obtains membranous milkvetch stem and leaf pollen；
(2) water extraction：By membranous milkvetch stem and leaf pollen and Chinese medical stone water in mass ratio 1：10 mixing, 25 minutes are incubated at 95 DEG C, is adopted
With 300 mesh filter-cloth filterings, first-time filtrate is obtained, the Chinese medical stone water of 4 times of membranous milkvetch stem and leaf pollen weight is added in filter residue, in 95 DEG C of guarantors
Temperature 25 minutes, using 300 mesh filter-cloth filterings, secondary filtrate is obtained, merges filtrate twice, be 50 DEG C in pressure 0.06MPa, temperature
Condition vacuum-concentrcted to 5 times of membranous milkvetch stem and leaf pollen weight, obtain Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：0.4：1 mixing, with 80
Rev/min stirring 10 minutes, obtain mixed bacteria liquid；
(4) ferment：8 parts of membranous milkvetch stem and leaf pollen, 30 parts of corn, 15 parts of wheat bran, 30 parts of peanut meal, rice bran are weighed by weight
15 parts, 2 parts of sucrose, 0.05 part of salt, fermentation raw material is obtained after well mixed, sprays the use of mixed bacteria liquid, wherein mixed bacteria liquid
Amount is the 10% of fermentation raw material quality, then is sealed by fermentation 4 days at 30 DEG C, obtains tunning；
(5) by tunning, nutrition recuperating agent, acidulant in mass ratio 100：8：0.05 mixing, then dry 20 at 50 DEG C
Hour, obtain the flower containing membranous milkvetch stem and leaf subtracts anti-feed.
The Chinese medical stone water is prepared by following methods：By medical stone and deionized water in mass ratio 1：8 mixing, 50
DEG C immersion 50 minutes, with 6000 revs/min centrifuge 5 minutes, take supernatant, obtain Chinese medical stone water.
The nutrition recuperating agent includes the raw material of following weight parts：25 parts of whey powder, 30 parts of Gluten, 30 parts of corn flour,
7 parts of glucose, 6 parts of calcium monohydrogen phosphate, 2 parts of soybean oil.The nutrition recuperating agent is prepared by following methods：By whey powder, paddy
Protein powder, corn flour, glucose, calcium monohydrogen phosphate, soybean oil are well mixed, and obtain nutrition recuperating agent.
The Mixed Microbes are bacillus subtilis, candida utili, lactobacillus acidophilus in mass ratio 2：0.5：1.5 it is mixed
Compound.
The acidulant is chlorogenic acid.
The fermentation accelerant is the mixture of polyglyceryl fatty acid ester and Tea Polyphenols, the polyglyceryl fatty acid ester and tea
The mass ratio of polyphenol is 3：1.
Meat chicken production performance test result：Average daily gain 104.83g, average daily gain 60.03g, feedstuff-meat ratio 1.75；
The Development of Immune Organs situation test result of broiler chicken：Index and spleen index 1.88, bursal index 1.37.
Embodiment 11
Subtract the method for anti-feed cultivation edible animal containing what the Radix Astragali or membranous milkvetch stem and leaf were spent, including use and contain the Radix Astragali or the Radix Astragali
Cauline leaf flower subtracts anti-feed raising birds.
1 Day-old Broiler Chickens are chosen, whole process is fed subtracts anti-feed containing the Radix Astragali.Sub-cage rearing, free choice feeding and drinking-water, routinely exempt from
Epidemic disease.
The preparation method for subtracting anti-feed containing the Radix Astragali comprises the following steps：
(1) Radix Astragali pre-processes：The root of the fresh Radix Astragali is gathered, is placed in 50 DEG C of drying boxes and dries 20 hours, is crushed as 80 mesh,
Obtain astragalus membranaceus powder；
(2) water extraction：By astragalus membranaceus powder and Chinese medical stone water in mass ratio 1：10 mixing, 25 minutes are incubated at 95 DEG C, using 300 mesh
Filter-cloth filtering, first-time filtrate is obtained, the Chinese medical stone water of 4 times of astragalus membranaceus powder weight is added in filter residue, 25 minutes is incubated at 95 DEG C, uses
300 mesh filter-cloth filterings, secondary filtrate is obtained, merge filtrate twice, subtracted in pressure 0.06MPa, the condition vacuum that temperature is 50 DEG C
Pressure is concentrated into 5 times of astragalus membranaceus powder weight, obtains Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：0.4：1 mixing, with 80
Rev/min stirring 10 minutes, obtain mixed bacteria liquid；
(4) ferment：Weigh by weight 8 parts of astragalus membranaceus powder, 30 parts of corn, 15 parts of wheat bran, 30 parts of peanut meal, 15 parts of rice bran,
2 parts of sucrose, 0.05 part of salt, fermentation raw material is obtained after well mixed, spray mixed bacteria liquid, the dosage of wherein mixed bacteria liquid is hair
The 10% of ferment material quality, then be sealed by fermentation 4 days at 30 DEG C, obtain tunning；
(5) by tunning, nutrition recuperating agent, acidulant in mass ratio 100：8：0.05 mixing, then dry 20 at 50 DEG C
Hour, obtain subtracting anti-feed containing the Radix Astragali.
The Chinese medical stone water is prepared by following methods：By medical stone and deionized water in mass ratio 1：8 mixing, 50
DEG C immersion 50 minutes, with 6000 revs/min centrifuge 5 minutes, take supernatant, obtain Chinese medical stone water.
The nutrition recuperating agent includes the raw material of following weight parts：25 parts of whey powder, 30 parts of Gluten, 30 parts of corn flour,
7 parts of glucose, 6 parts of calcium monohydrogen phosphate, 2 parts of soybean oil.The nutrition recuperating agent is prepared by following methods：By whey powder, paddy
Protein powder, corn flour, glucose, calcium monohydrogen phosphate, soybean oil are well mixed, and obtain nutrition recuperating agent.
The Mixed Microbes are bacillus subtilis, candida utili, lactobacillus acidophilus in mass ratio 2：0.5：1.5 it is mixed
Compound.
The acidulant is chlorogenic acid.
The fermentation accelerant is the mixture of polyglyceryl fatty acid ester and Tea Polyphenols, the polyglyceryl fatty acid ester and tea
The mass ratio of polyphenol is 3：1.
Comparative example 1
1 Day-old Broiler Chickens are chosen, 510 broiler chicken mixed feed early stage (the limited public affairs of Xuzhou science-star's development in science and technology are fed in 1-21 days
Department), 21 ages in days use 511 broiler chicken mid-term mixed feeds (Xuzhou science-star's development in science and technology Co., Ltd) afterwards.Sub-cage rearing, freely
Feeding and drinking-water, routine immunization.
Test case 1
Each embodiment broiler health is in order, anti-with subtracting for the obtained flowers containing membranous milkvetch stem and leaf of embodiment 1-8 during experiment
Forage feed broiler chicken, tests the production performance of broiler chicken, and concrete outcome is shown in Table 1.
The production performance test result table of the broiler chicken of table 1
| Average daily gain/g | Average daily gain/g | Feedstuff-meat ratio | |
| Embodiment 1 | 100.36 | 52.07 | 1.93 |
| Embodiment 2 | 101.03 | 53.36 | 1.89 |
| Embodiment 3 | 101.85 | 55.43 | 1.84 |
| Embodiment 4 | 101.76 | 55.39 | 1.84 |
| Embodiment 5 | 101.69 | 55.03 | 1.85 |
| Embodiment 6 | 101.73 | 55.48 | 1.83 |
| Embodiment 7 | 102.23 | 56.42 | 1.81 |
| Embodiment 8 | 103.06 | 57.85 | 1.78 |
| Comparative example 1 | 100.28 | 49.35 | 2.03 |
Embodiment 2 prepares mixed bacteria liquid using Aqueous extracts, and the obtained flower containing membranous milkvetch stem and leaf subtracts anti-forage feed broiler chicken, meat
The average daily gain of chicken and feedstuff-meat ratio are superior to embodiment 1, and this is due to that some active components in Radix Astragali extractive solution are advantageous to
The growth of zymogenous bacteria, so as to improve the quality of tunning.
Embodiment 3 prepares Aqueous extracts using Chinese medical stone water, and the obtained flower containing membranous milkvetch stem and leaf subtracts anti-forage feed broiler chicken, meat
The average daily gain of chicken and feedstuff-meat ratio are superior to embodiment 2, because Chinese medical stone water can promote the progress of fermentation reaction, and
And broiler chicken can be made preferably to absorb trace element with Chinese medical stone water.
Embodiment 3-8 optimizes screening to acidulant, and subtracting for the flower containing membranous milkvetch stem and leaf for finding to obtain using chlorogenic acid is anti-
Forage feed broiler chicken, the average daily gain of broiler chicken and feedstuff-meat ratio are superior to other several acid.This is probably because chlorogenic acid can be with
Preferably activation propepsin, digestive enzyme activity is improved, nutriment is preferably absorbed, so as to improve performance of poultry
And feed conversion rate.
Test case 2
Subtract anti-forage feed broiler chicken containing what membranous milkvetch stem and leaf was spent with what embodiment 1-8 was obtained, test the immune organ hair of broiler chicken
Educate situation, all broiler chicken immune organs butchered no abnormality seen in appearance.Concrete outcome is shown in Table 2.
The Development of Immune Organs situation test result table of the broiler chicken of table 2
| Index and spleen index, g/Kg | Bursal index, g/Kg | |
| Embodiment 1 | 1.69 | 1.07 |
| Embodiment 2 | 1.71 | 1.14 |
| Embodiment 3 | 1.75 | 1.22 |
| Embodiment 4 | 1.73 | 1.19 |
| Embodiment 5 | 1.74 | 1.19 |
| Embodiment 6 | 1.76 | 1.21 |
| Embodiment 7 | 1.77 | 1.23 |
| Embodiment 8 | 1.82 | 1.30 |
| Comparative example 1 | 1.57 | 1.03 |
Claims (10)
1. the method for subtracting anti-feed cultivation edible animal spent containing the Radix Astragali or membranous milkvetch stem and leaf, it is characterised in that contain including using
The anti-feed that subtracts of the Radix Astragali or membranous milkvetch stem and leaf flower raises edible animal.
2. the method for subtracting anti-feed cultivation edible animal spent as claimed in claim 1 containing the Radix Astragali or membranous milkvetch stem and leaf, its feature
It is, the preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 40-60 DEG C of drying box and dries 18-24 hours,
Crush as 60-100 mesh, obtain membranous milkvetch stem and leaf pollen；
(2) mixed bacteria liquid is prepared：By deionized water, Mixed Microbes, fermentation accelerant in mass ratio 100：(0.1-0.5)：(0.5-
1.5) mix, with 60-100 revs/min of stirring 8-12 minute, obtain mixed bacteria liquid；
(3) ferment：Membranous milkvetch stem and leaf pollen 5-10 parts, corn 25-35 parts, wheat bran 10-20 parts, peanut meal 25- are weighed by weight
35 parts, rice bran 10-20 parts, sucrose 1-5 parts, salt 0.01-0.2 parts, fermentation raw material is obtained after well mixed, sprays Mixed Microbes
The dosage of liquid, wherein mixed bacteria liquid is the 5-15% of fermentation raw material quality, then is sealed by fermentation 3-5 days at 25-35 DEG C, is fermented
Product；
(4) batch mixing：By tunning, nutrition recuperating agent, acidulant in mass ratio 100：(5-10)：(0.01-0.1) is mixed, then
In 40-60 DEG C of dry 18-24 hour, obtain the flower containing membranous milkvetch stem and leaf subtracts anti-feed.
3. the method for subtracting anti-feed cultivation edible animal spent as claimed in claim 2 containing the Radix Astragali or membranous milkvetch stem and leaf, its feature
It is, the preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 40-60 DEG C of drying box and dries 18-24 hours,
Crush as 60-100 mesh, obtain membranous milkvetch stem and leaf pollen；
(2) water extraction：By membranous milkvetch stem and leaf pollen and deionized water in mass ratio 1：(8-12) is mixed, and 20-30 is incubated at 90-100 DEG C
Minute, using 300 mesh filter-cloth filterings, first-time filtrate is obtained, the deionization of 3-5 times of membranous milkvetch stem and leaf pollen weight is added in filter residue
Water, 20-30 minutes are incubated at 90-100 DEG C, using 300 mesh filter-cloth filterings, obtain secondary filtrate, merge filtrate twice, in 40-
60 DEG C of be concentrated under reduced pressure into membranous milkvetch stem and leaf pollen weight 4-6 times, obtain Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：(0.1-0.5)：(0.5-1.5)
Mixing, with 60-100 revs/min of stirring 8-12 minute, obtain mixed bacteria liquid；
(4) ferment：Membranous milkvetch stem and leaf pollen 5-10 parts, corn 25-35 parts, wheat bran 10-20 parts, peanut meal 25- are weighed by weight
35 parts, rice bran 10-20 parts, sucrose 1-5 parts, salt 0.01-0.2 parts, fermentation raw material is obtained after well mixed, sprays Mixed Microbes
The dosage of liquid, wherein mixed bacteria liquid is the 5-15% of fermentation raw material quality, then is sealed by fermentation 3-5 days at 25-35 DEG C, is fermented
Product；
(5) batch mixing：By tunning, nutrition recuperating agent, acidulant in mass ratio 100：(5-10)：(0.01-0.1) is mixed, then
In 40-60 DEG C of dry 18-24 hour, obtain the flower containing membranous milkvetch stem and leaf subtracts anti-feed.
4. the method for subtracting anti-feed cultivation edible animal spent as claimed in claim 2 containing the Radix Astragali or membranous milkvetch stem and leaf, its feature
It is, the preparation method for subtracting anti-feed of the flower containing membranous milkvetch stem and leaf comprises the following steps：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 40-60 DEG C of drying box and dries 18-24 hours,
Crush as 60-100 mesh, obtain membranous milkvetch stem and leaf pollen；
(2) water extraction：By membranous milkvetch stem and leaf pollen and Chinese medical stone water in mass ratio 1：(8-12) is mixed, and 20-30 is incubated at 90-100 DEG C
Minute, using 300 mesh filter-cloth filterings, first-time filtrate is obtained, the Chinese medical stone water of 3-5 times of membranous milkvetch stem and leaf pollen weight is added in filter residue
20-30 minutes are incubated at 90-100 DEG C, using 300 mesh filter-cloth filterings, secondary filtrate is obtained, merges filtrate twice, at 40-60 DEG C
4-6 times of membranous milkvetch stem and leaf pollen weight is concentrated under reduced pressure into, obtains Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：(0.1-0.5)：(0.5-1.5)
Mixing, with 60-100 revs/min of stirring 8-12 minute, obtain mixed bacteria liquid；
(4) ferment：Membranous milkvetch stem and leaf pollen 5-10 parts, corn 25-35 parts, wheat bran 10-20 parts, peanut meal 25- are weighed by weight
35 parts, rice bran 10-20 parts, sucrose 1-5 parts, salt 0.01-0.2 parts, fermentation raw material is obtained after well mixed, sprays Mixed Microbes
The dosage of liquid, wherein mixed bacteria liquid is the 5-15% of fermentation raw material quality, then is sealed by fermentation 3-5 days at 25-35 DEG C, is fermented
Product；
(5) batch mixing：By tunning, nutrition recuperating agent, acidulant in mass ratio 100：(5-10)：(0.01-0.1) is mixed, then
In 40-60 DEG C of dry 18-24 hour, obtain the flower containing membranous milkvetch stem and leaf subtracts anti-feed.
5. the method for subtracting anti-feed cultivation edible animal spent as claimed in claim 4 containing the Radix Astragali or membranous milkvetch stem and leaf, its feature
It is, the fermentation accelerant is polyglyceryl fatty acid ester and/or Tea Polyphenols.
6. the method for subtracting anti-feed cultivation edible animal spent as claimed in claim 4 containing the Radix Astragali or membranous milkvetch stem and leaf, its feature
It is, the Chinese medical stone water is prepared by following methods：By medical stone and deionized water in mass ratio 1：(5-10) is mixed,
45-55 DEG C of immersion 40-60 minute, with 4000-8000 revs/min of centrifugation 4-8 minute, supernatant is taken, obtains Chinese medical stone water.
7. the method for subtracting anti-feed cultivation edible animal spent as claimed in claim 4 containing the Radix Astragali or membranous milkvetch stem and leaf, its feature
It is, the nutrition recuperating agent includes the raw material of following weight parts：Whey powder 20-30 parts, Gluten 25-35 parts, corn flour 25-
35 parts, glucose 5-10 parts, calcium monohydrogen phosphate 4-8 parts, soybean oil 1-3 parts.
8. the method for subtracting anti-feed cultivation edible animal spent as claimed in claim 4 containing the Radix Astragali or membranous milkvetch stem and leaf, its feature
It is, the Mixed Microbes are bacillus subtilis, candida utili, lactobacillus acidophilus (1-3) in mass ratio：(0.1-1)(1-
2) mixture.
9. the side for subtracting anti-feed cultivation edible animal spent as described in claim any one of 1-8 containing the Radix Astragali or membranous milkvetch stem and leaf
Method, it is characterised in that the edible animal includes birds, domestic animals, fishing class, and the preferable birds include family chicken (Callus
Domesticus) cock, turkey (Meleagris gllaopavo), guinea fowl (Numida meleagris), dove (Columba
Livia), duck (Anas Platyrhynchus var.domestica), goose (Anas cygnoides)；The domestic animal includes
Pig, ox, sheep, horse, camel, rabbit, dog；The fishing class includes fish, shrimp, crab, soft-shelled turtle, shellfish, Echinodermata, software class, coelenteron class, amphibious
Class, extra large beast-like animals.
10. a kind of flower containing membranous milkvetch stem and leaf subtracts anti-feed, it is characterised in that is prepared using following methods：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 40-60 DEG C of drying box and dries 18-24 hours,
Crush as 60-100 mesh, obtain membranous milkvetch stem and leaf pollen；
(2) mixed bacteria liquid is prepared：By deionized water, Mixed Microbes, fermentation accelerant in mass ratio 100：(0.1-0.5)：(0.5-
1.5) mix, with 60-100 revs/min of stirring 8-12 minute, obtain mixed bacteria liquid；
(3) ferment：Membranous milkvetch stem and leaf pollen 5-10 parts, corn 25-35 parts, wheat bran 10-20 parts, peanut meal 25- are weighed by weight
35 parts, rice bran 10-20 parts, sucrose 1-5 parts, salt 0.01-0.2 parts, fermentation raw material is obtained after well mixed, sprays Mixed Microbes
The dosage of liquid, wherein mixed bacteria liquid is the 5-15% of fermentation raw material quality, then is sealed by fermentation 3-5 days at 25-35 DEG C, is fermented
Product；
(4) batch mixing：By tunning, nutrition recuperating agent, acidulant in mass ratio 100：(5-10)：(0.01-0.1) is mixed, then
In 40-60 DEG C of dry 18-24 hour, obtain the flower containing membranous milkvetch stem and leaf subtracts anti-feed；
Preferably, it is prepared using following methods：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 40-60 DEG C of drying box and dries 18-24 hours,
Crush as 60-100 mesh, obtain membranous milkvetch stem and leaf pollen；
(2) water extraction：By membranous milkvetch stem and leaf pollen and deionized water in mass ratio 1：(8-12) is mixed, and 20-30 is incubated at 90-100 DEG C
Minute, using 300 mesh filter-cloth filterings, first-time filtrate is obtained, the deionization of 3-5 times of membranous milkvetch stem and leaf pollen weight is added in filter residue
Water, 20-30 minutes are incubated at 90-100 DEG C, using 300 mesh filter-cloth filterings, obtain secondary filtrate, merge filtrate twice, in 40-
60 DEG C of be concentrated under reduced pressure into membranous milkvetch stem and leaf pollen weight 4-6 times, obtain Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：(0.1-0.5)：(0.5-1.5)
Mixing, with 60-100 revs/min of stirring 8-12 minute, obtain mixed bacteria liquid；
(4) ferment：Membranous milkvetch stem and leaf pollen 5-10 parts, corn 25-35 parts, wheat bran 10-20 parts, peanut meal 25- are weighed by weight
35 parts, rice bran 10-20 parts, sucrose 1-5 parts, salt 0.01-0.2 parts, fermentation raw material is obtained after well mixed, sprays Mixed Microbes
The dosage of liquid, wherein mixed bacteria liquid is the 5-15% of fermentation raw material quality, then is sealed by fermentation 3-5 days at 25-35 DEG C, is fermented
Product；
(5) batch mixing：By tunning, nutrition recuperating agent, acidulant in mass ratio 100：(5-10)：(0.01-0.1) is mixed, then
In 40-60 DEG C of dry 18-24 hour, obtain the flower containing membranous milkvetch stem and leaf subtracts anti-feed；
Optimal, it is prepared using following methods：
(1) membranous milkvetch stem and leaf flower pretreatment：Fresh membranous milkvetch stem and leaf flower is gathered, is placed in 40-60 DEG C of drying box and dries 18-24 hours,
Crush as 60-100 mesh, obtain membranous milkvetch stem and leaf pollen；
(2) water extraction：By membranous milkvetch stem and leaf pollen and Chinese medical stone water in mass ratio 1：(8-12) is mixed, and 20-30 is incubated at 90-100 DEG C
Minute, using 300 mesh filter-cloth filterings, first-time filtrate is obtained, the Chinese medical stone water of 3-5 times of membranous milkvetch stem and leaf pollen weight is added in filter residue
20-30 minutes are incubated at 90-100 DEG C, using 300 mesh filter-cloth filterings, secondary filtrate is obtained, merges filtrate twice, at 40-60 DEG C
4-6 times of membranous milkvetch stem and leaf pollen weight is concentrated under reduced pressure into, obtains Aqueous extracts；
(3) mixed bacteria liquid is prepared：By Aqueous extracts, Mixed Microbes, fermentation accelerant in mass ratio 100：(0.1-0.5)：(0.5-1.5)
Mixing, with 60-100 revs/min of stirring 8-12 minute, obtain mixed bacteria liquid；
(4) ferment：Membranous milkvetch stem and leaf pollen 5-10 parts, corn 25-35 parts, wheat bran 10-20 parts, peanut meal 25- are weighed by weight
35 parts, rice bran 10-20 parts, sucrose 1-5 parts, salt 0.01-0.2 parts, fermentation raw material is obtained after well mixed, sprays Mixed Microbes
The dosage of liquid, wherein mixed bacteria liquid is the 5-15% of fermentation raw material quality, then is sealed by fermentation 3-5 days at 25-35 DEG C, is fermented
Product；
(5) batch mixing：By tunning, nutrition recuperating agent, acidulant in mass ratio 100：(5-10)：(0.01-0.1) is mixed, then
In 40-60 DEG C of dry 18-24 hour, obtain the flower containing membranous milkvetch stem and leaf subtracts anti-feed.
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| CN108576424A (en) * | 2018-05-21 | 2018-09-28 | 侯宝良 | Improve the yak fermented feed of growth performance |
| CN109221598A (en) * | 2018-11-26 | 2019-01-18 | 大兴安岭北天原生物科技有限责任公司 | Subtract anti-feed addictive and its processing method containing Radix Astragali or Radix Astragali stem, leaf, flower |
| CN109221622A (en) * | 2018-11-26 | 2019-01-18 | 大兴安岭北天原生物科技有限责任公司 | Subtract anti-feed and preparation method thereof containing Radix Astragali or Radix Astragali stem, leaf, flower |
| CN111789186A (en) * | 2020-06-19 | 2020-10-20 | 山西省繁峙县天河牧业有限公司 | Fermented astragalus membranaceus and preparation method thereof, feed additive and preparation method thereof, and beef cattle feed |
| CN113841799A (en) * | 2021-09-24 | 2021-12-28 | 盐城师范学院 | Pig feed containing bunge auriculate root stems and leaves as well as preparation method and application of pig feed |
| CN115777832A (en) * | 2022-11-16 | 2023-03-14 | 南京中医药大学 | Preparation method and application of astragalus stem and leaf mixed solid fermentation product |
| CN116439315A (en) * | 2023-04-21 | 2023-07-18 | 南京中医药大学 | Clostridium butyricum fermentation product of astragalus membranaceus stem and leaf as well as preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108576424A (en) * | 2018-05-21 | 2018-09-28 | 侯宝良 | Improve the yak fermented feed of growth performance |
| CN109221598A (en) * | 2018-11-26 | 2019-01-18 | 大兴安岭北天原生物科技有限责任公司 | Subtract anti-feed addictive and its processing method containing Radix Astragali or Radix Astragali stem, leaf, flower |
| CN109221622A (en) * | 2018-11-26 | 2019-01-18 | 大兴安岭北天原生物科技有限责任公司 | Subtract anti-feed and preparation method thereof containing Radix Astragali or Radix Astragali stem, leaf, flower |
| CN109221622B (en) * | 2018-11-26 | 2021-09-07 | 大兴安岭北天原生物科技有限责任公司 | An anti-disease feed containing radix astragali or stem, leaf and flower of radix astragali, and its preparation method |
| CN111789186A (en) * | 2020-06-19 | 2020-10-20 | 山西省繁峙县天河牧业有限公司 | Fermented astragalus membranaceus and preparation method thereof, feed additive and preparation method thereof, and beef cattle feed |
| CN111789186B (en) * | 2020-06-19 | 2023-12-15 | 山西省繁峙县天河牧业有限公司 | Fermented astragalus root and preparation method thereof, feed additive and preparation method thereof, and beef cattle feed |
| CN113841799A (en) * | 2021-09-24 | 2021-12-28 | 盐城师范学院 | Pig feed containing bunge auriculate root stems and leaves as well as preparation method and application of pig feed |
| CN113841799B (en) * | 2021-09-24 | 2023-10-27 | 盐城师范学院 | Pig feed containing bunge auriculate root stems and leaves as well as preparation method and application thereof |
| CN115777832A (en) * | 2022-11-16 | 2023-03-14 | 南京中医药大学 | Preparation method and application of astragalus stem and leaf mixed solid fermentation product |
| CN116439315A (en) * | 2023-04-21 | 2023-07-18 | 南京中医药大学 | Clostridium butyricum fermentation product of astragalus membranaceus stem and leaf as well as preparation method and application thereof |
| CN116439315B (en) * | 2023-04-21 | 2024-04-26 | 南京中医药大学 | Clostridium butyricum fermentation product of astragalus membranaceus stem and leaf as well as preparation method and application thereof |
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