CN107485644A - A kind of antibacterial anti-inflammatory agent and its application - Google Patents
A kind of antibacterial anti-inflammatory agent and its application Download PDFInfo
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- CN107485644A CN107485644A CN201710696340.6A CN201710696340A CN107485644A CN 107485644 A CN107485644 A CN 107485644A CN 201710696340 A CN201710696340 A CN 201710696340A CN 107485644 A CN107485644 A CN 107485644A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D9/00—Compositions of detergents based essentially on soap
- C11D9/04—Compositions of detergents based essentially on soap containing compounding ingredients other than soaps
- C11D9/22—Organic compounds, e.g. vitamins
- C11D9/225—Polymers
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D9/00—Compositions of detergents based essentially on soap
- C11D9/04—Compositions of detergents based essentially on soap containing compounding ingredients other than soaps
- C11D9/22—Organic compounds, e.g. vitamins
- C11D9/26—Organic compounds, e.g. vitamins containing oxygen
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D9/00—Compositions of detergents based essentially on soap
- C11D9/04—Compositions of detergents based essentially on soap containing compounding ingredients other than soaps
- C11D9/22—Organic compounds, e.g. vitamins
- C11D9/26—Organic compounds, e.g. vitamins containing oxygen
- C11D9/265—Organic compounds, e.g. vitamins containing oxygen containing glycerol
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D9/00—Compositions of detergents based essentially on soap
- C11D9/04—Compositions of detergents based essentially on soap containing compounding ingredients other than soaps
- C11D9/22—Organic compounds, e.g. vitamins
- C11D9/38—Products in which the composition is not well defined
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Microbiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Medicines Containing Plant Substances (AREA)
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Abstract
The invention discloses a kind of antibacterial anti-inflammatory agent and its application, the antibacterial anti-inflammatory agent is made up of following parts by weight of component：Mulberry leaf extract 0.4 3.5 parts of slag, granatum 0.3 3.7 parts of slag of extraction, balsam pear 0.3 3.5 parts of slag of extraction.It is applied in handmade soap, there is antibacterial anti-inflammatory and other effects, and product of the present invention effectively utilizes plant residue, turns waste into wealth, reduces production cost, improves the utilization rate of plant material.
Description
Technical field
The present invention relates to cosmetic field, and in particular to a kind of antibacterial anti-inflammatory agent and its application.
Background technology
Soap is the washing product generally used, there is stronger cleaning capacity to skin, and cheap durable.But current soap
The glycerine that saponification obtains in process of production can be typically pulled out, and easily cause dry skin, coarse.Grease after handmade soap saponification
It is not pulled out, there is more preferable moistening effect, more gently, thus handmade soap is more suitable for skin cleaning.Existing handmade soap,
To improve combination property, such as antibiotic property, spatter property etc. can add the material of chemical synthesis, such as antiseptic, activating agent etc., long
Phase to skin using easily causing to stimulate, or even causes allergic reaction.Therefore, it is badly in need of exploitation a kind of natural antibacterial, antiinflammatory,
The composition naturally extracted is gently non-stimulated.
The content of the invention
Based on this, it is an object of the invention to provide a kind of antibacterial anti-inflammatory agent, it is thin that it reduces inflammation by antibacterial bacteriostatic
The breeding of bacterium fungi, play antibacterial anti-inflammatory effect.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of antibacterial anti-inflammatory agent extracts slag by mulberry leaf, granatum extracts slag and balsam pear extraction slag forms.
Further, described antibacterial anti-inflammatory agent includes following parts by weight of component：Mulberry leaf extract slag 0.4-3.5 parts, stone
Pomegranate skin extracts slag 0.3-3.7 parts, balsam pear extraction slag 0.3-3.5 parts.
Preferably, described antibacterial anti-inflammatory agent includes following parts by weight of component：Mulberry leaf extract slag 0.6-3.2 parts, pomegranate
Skin extracts slag 0.6-3.5 parts, balsam pear extraction slag 0.5-3.0 parts.
Optimal, described antibacterial anti-inflammatory agent includes the component of following parts by weight：Mulberry leaf extract slag 1.5-2.9 parts, pomegranate
Skin extracts slag 1.2-3.0 parts, balsam pear extraction slag 1.0-2.7 parts.
A kind of preparation method of antibacterial anti-inflammatory agent is as follows：Mulberry leaf, granatum and balsam pear plant material are collected by extraction profit
Plant residue raw material after, and cleaned, dried respectively, 10-15 mesh is then respectively crushed into, finally according to aforementioned proportion
Mixing.
Further, the plant residue raw material after extraction and application refers to mulberry leaf, granatum and balsam pear plant
Raw material filters out after one or more technique extraction and applications in alcohol extracting, water extraction, immersion method and carbon dioxide extraction method
Residue.
The present invention also provides a kind of application of antibacterial anti-inflammatory agent in cosmetics are prepared, and the cosmetics are handmade soap.
Further, described handmade soap includes following parts by weight of component：Above-mentioned antibacterial anti-inflammatory agent 3.7-8.6 parts,
Laurate 4.2-7.8 parts, sorb (sugar) alcohol 6.5-12 parts, palmitic acid 3.4-7.3 parts, sodium hydroxide 0.1-0.5 parts, coconut oil
3.5-8.0 parts, Sodium Hyaluronate 0.01-0.05 parts, glycerine 2.1-5.4 parts, PEG-7 dimethyl silicone polymer 0.6-1.7 parts, benzene
Oxyethanol 0.1-0.5 parts, Sensiva SC50 0.1-0.3 parts, deionized water surplus.
Several components in the plant residue composition used in the present invention are introduced below：
Mulberry leaf are the dried leafs of moraceae plants mulberry, are a kind of conventional wind-heat-dispersing medicinals, can take orally, also can external application.Mulberry
Leaf first recorded in《Sheng Nong's herbal classic》, bitter, sweet, cold in nature, return lung, Liver Channel；Function dispelling wind and heat from the body, clearing away the lungheat and moisturizing, clear liver and improve vision；Contain
There are abundant amino acid, vitamin, mineral matter, cellulose and several physiological active substances.The pharmacological action of mulberry leaf is very wide
It is general, there is anti-inflammatory, antipyretic, analgesia, antianxiety, anti-dopamine, immunological regulation, suppress melanin, anti-snake venom, treat snail fever
Deng pharmacological action.Flavone compound, polyphenol compound in mulberry leaf, which are respectively provided with, removes free radical and anti-aging effects.Efficiently
Liquid chromatography (HPLC) analysis mulberry leaf methanolic extract contains the main components such as morin, Anisodus luridus and umbelliferone,
Its extract suppresses cyclooxygenase -1 (COX1) and cyclooxygenase-2 (COX2) and 5- LOXs (5-LOX), double inhibition flower
Raw tetraenoic acid approach, so as to produce antipyretic and analgesic activity.High performance liquid chromatography carries out flavonoids in analysis mulberry-leaf extract
Compound, three kinds of Quercetin (QC), Kaempferol (KF), astragalin (AG) flavone compounds are included in mulberry-leaf extract.AG exists
The inhibition of haemolysis is more preferable than other flavonoids, and KF and QC have anti-oxidant and anti-inflammatory activity, and can prevent red blood cell from aoxidizing
Damage.Mulberry leaf can reduce oxidation and c reactive protein (CRP) level of mile abnormality dyslipidemia patient, and make with anti-inflammatory
With.
Granatum is the drying pericarp of Punicaceae plant pomegranate.Its property is sour, warm, puckery, has relieving diarrhea with astringents, hemostasis, expelling parasite
Deng effect, for chronic diarrhea, protracted dysentery, have blood in stool, prolapse of the anus, uterine bleeding, leukorrhea, abdominal pain due to enterositosis.Because blood such as drops in its unique physiologically active
Fat, anti-oxidant, antiviral, antitumor, hypoglycemic, anti diar rhea and antibacterial etc., in recent years as the focus of scientist's research.Modern medicine
Pharmacological research shows that the Antibacterial Constituents in granatum are flavonoids and tannins.The tannin of granatum is because of its energy
The plasm of microbial body and a variety of enzymes are solidified, has inhibitory action to various bacteriums and fungi.Zhou Benhong etc. is to tan in granatum
Matter each component carries out clinical research to gonococcus sensitiveness, it was demonstrated that granatum has obvious inhibitory action to gonococcus.Stone among the people
Pomegranate skin is often used by people to treat diarrhoea, dysentery, aphtha, abscess, pharyngalgia, urinary tract inflammation, scabies, skin injury and hemostasis etc..
After flavonoids in granatum and tannin constituents are carried out extraction separation by Yang Lin etc., In Vitro Bacteriostatic experimental study is carried out.
As a result show, tannins and Flavonoids are to staphylococcus aureus, Escherichia coli, Salmonella in granatum
Bacterium, shigella flexneri, Pseudomonas aeruginosa and Candida albicans all have certain inhibitory action, and tannins show
Go out spectrum antibacterial characteristics.
Balsam pear is cucurbitaceous plant.Balsam pear is more universal as the purposes of vegetables, and its pharmacological research is only recent decades
Thing, confirm that it has treatment diabetes, antiviral, anticancer, anti-leukocythemia, antibacterial and desinsection by advanced experimental method
Deng effect.Restraining and sterilizing bacteria effect is observed with balsam pear liquid (5g/ml), the results showed that：Balsam pear liquid is to gram-positive cocci, gram
Positive bacillus and negative bacillus all have antibacterial action, and all minimum inhibitory concentrations (MIC) and minimum bactericidal to cell
Concentration (MBC) is 1250-2500mg/ml scopes, and control group has cell growth.Balsam pear liquid is only to Candida albicans without work
With.Balsam pear has the effect such as antibacterial, hypoglycemic, antifertility, anti-mutation, immunological regulation.
Compared with prior art, it is the device have the advantages that as follows：Product of the present invention is using mulberry leaf extraction slag, pomegranate
Skin extraction slag, balsam pear extract slag compounding use in specific proportions, 3 kinds of composition cooperative effects, inflammation are reduced by antibacterial bacteriostatic
The breeding of bacterial fungus, play antibacterial anti-inflammatory effect.Mulberry leaf extraction slag, granatum extraction slag, balsam pear extraction slag three-in-one, hair
Strong bacteriostasis efficacy is waved, act synergistically more effective antiinflammatory action.In addition, product of the present invention also containing sorb (sugar) alcohol,
The NMFs such as Sodium Hyaluronate, coconut oil, water lock moisturizing effectively is carried out to skin.The present invention mainly also has using in residue
The distinctive active component of its raw material, the effect of synergy to reach antibacterial anti-inflammatory.These residues are effectively utilized, are turned waste into wealth,
Production cost is reduced, improves the utilization rate of plant material, while contributes to environmental protection.
Embodiment
Below in conjunction with specific embodiment, the present invention is further elaborated：
The handmade soap embodiment 1~5 of the present invention is formulated, and is shown in Table 1.
Table 1
The preparation method of antibacterial anti-inflammatory agent is as follows：
The plant residue raw material of mulberry leaf, granatum and balsam pear plant material after extraction and application is collected, and is carried out respectively
Removal of impurities, dry, be then respectively crushed into 10-15 mesh, finally mixed according to aforementioned proportion.
Comparative example 1：
Mulberry leaf extraction slag is not added in difference with embodiment 3 only, and remaining each material addition, conditions and technology of preparation is not
Change.
Comparative example 2：
Granatum extraction slag is not added in difference with embodiment 3 only, remaining each material addition, and conditions and technology of preparation is equal
Do not change.
Comparative example 3：
Balsam pear extraction slag is not added in difference with embodiment 3 only, and remaining each material addition, conditions and technology of preparation is not
Change.
Comparative example 4：
Compared with Example 3, mulberry leaf extraction slag, granatum extraction slag, balsam pear extraction slag do not add, and remaining each material adds
Dosage, conditions and technology of preparation do not change still.
Antibiotic effect is verified：
(1) experimental strain:
Propionibacterium acnes (ATCC 6919), Fusobacterium nucleatum (ATCC 25586), Escherichia coli (ATCC 25922).
(above purchased from Guangdong Province's Organism Depositary)
(2) operating procedure：
A. above-mentioned three kinds of pathogenic bacteria are taken, are inoculated on sabouraud culture medium, in corresponding incubator, 38 DEG C, are cultivated 72 hours；
B. the bacterium solution after re-activation is taken, 10 are diluted to Sharpe fluid nutrient medium8CFU/mL；
C. the bacterium solution after dilution is added in 96 orifice plates, 100 microlitres is added per hole, is hereafter added by doubling dilution
100 microlitres of handmade soap samples dissolved with dimethyl sulfoxide (DMSO) (DMSO), the antibiotic to add equivalent are put into as positive control
Incubator, cultivated 24~72 hours under the conditions of 37 DEG C；
D. its minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MFC) are determined according to long bacterium situation.
(3) experimental result is as shown in table 2：
Table 2
Experimental result is shown：Basic handmade soap formula is without the effect for suppressing bacterium；It is antibacterial anti-by the addition present invention
There is stronger inhibitory action after oxygen agent to skin pathomycete.
Anti-inflammatory efficacy is verified：
First, material and method
1st, animal：SD healthy rats 100,150 ± 30g of body weight, male and female half and half.
2nd, medicine：According to handmade soap made of the embodiment of the present invention 1~5, the raw material of comparative example 1~4 and method.
3rd, animal packet：Animal is randomly divided into 10 groups, every group 10, correspond to respectively using handmade soap made of each formula with
Using only the control group of physiological saline.
2nd, experimental method：Each group rat performs mark with marking pen in right ankle-joint, and determines right ankle with special tape measure
The Zhou Jing in joint.With the egg physiological salt liquid 0.1ml/ of Fresh 10% only, from the right side after ball portion to skin around ankle-joint
Lower injection cause is scorching, and cleaning rat using above-mentioned handmade soap or physiological saline respectively every 30min in 4 hours after cause is scorching causes inflammation foot
Portion, and right ankle and foot Zhou Jing is determined after Yu Zhiyan at 1 hour and 4 hours, calculate swelling.It the results are shown in Table 3.
3rd, result：
3 each handmade soap of table causes the influence (X ± SD) of foot swelling to rat egg white
Compared with control group, * P ＜ 0.05, * * P ＜ 0.01.
From upper table 3：
(1) handmade soap for adding antibacterial anti-inflammatory agent of the present invention causes rat paw edema to egg white, has within 4 hours after inflammation is caused obvious
Inhibitory action, compared with control group, each embodiment group is respectively provided with significant difference (P ＜ 0.05 or P ＜ 0.01), wherein with reality
Apply that the action of the handmade soap of example 3 is most fast, and anti-inflammation detumescence effect is the most obvious.
(2) compared with Example 3, comparative example 1~3 lacks some components, and the effect during its anti-inflammatory is imitated decreases, and right
Ratio 4 any composition for not adding the antibacterial antioxidant of the present invention completely, then it does not have antiphlogistic effects completely, and data are given birth to only using
The control group for managing salt solution is close.
2 points of the above illustrates, using handmade soap product made of the antibacterial antioxidant of the present invention, have and alleviate skin well
The effect of inflammation, the reason for this is that each composition is respectively provided with anti-inflammatory power, and synergy is there are between each composition, can further repaired
The cellular damage of skin part, promote inflammatory resolution.
Above example is merely to illustrate embodiment of the present invention, does not have any formal limit to the present invention
System；It is any to be engaged in professional and technical personnel, all have the ability using the technology contents having revealed that, some are made to it and changes or repaiies
Decorations, turn into the equivalent embodiment of equivalent variations.According to the technology of the present invention essence, simply modification, equivalent variations are carried out to the present invention
With modification, belong in the range of technical solution of the present invention.
Claims (9)
1. a kind of antibacterial anti-inflammatory agent, it is characterised in that described antibacterial anti-inflammatory agent extracts slag by mulberry leaf, granatum extracts slag and hardship
Melon extraction slag composition.
2. a kind of antibacterial anti-inflammatory agent according to claim 1, it is characterised in that described antibacterial anti-inflammatory agent includes following
Parts by weight of component：
Mulberry leaf extract slag 0.4-3.5 parts
Granatum extracts slag 0.3-3.7 parts
Balsam pear extracts slag 0.3-3.5 parts.
3. a kind of antibacterial anti-inflammatory agent according to claim 2, it is characterised in that described antibacterial anti-inflammatory agent includes following
Parts by weight of component：
Mulberry leaf extract slag 0.6-3.2 parts
Granatum extracts slag 0.6-3.5 parts
Balsam pear extracts slag 0.5-3.0 parts.
4. a kind of antibacterial anti-inflammatory agent according to claim 3, it is characterised in that described antibacterial anti-inflammatory agent includes following weight
Measure the component of part:
Mulberry leaf extract slag 1.5-2.9 parts
Granatum extracts slag 1.2-3.0 parts
Balsam pear extracts slag 1.0-2.7 parts.
5. a kind of antibacterial anti-inflammatory agent according to claim any one of 1-4, it is characterised in that described antibacterial anti-inflammatory agent
Preparation method is as follows：The plant residue raw material of mulberry leaf, granatum and balsam pear plant material after extraction and application is collected, and respectively
Cleaned, dried, be then respectively crushed into 10-15 mesh, finally mixed according to aforementioned proportion.
A kind of 6. antibacterial anti-inflammatory agent according to claim 5, it is characterised in that the plant after extraction and application
Residuum feedstocks refer to that mulberry leaf, granatum and balsam pear plant material pass through in alcohol extracting, water extraction, immersion method and carbon dioxide extraction method
After one or more technique extraction and applications, the residue that filters out.
A kind of 7. application of antibacterial anti-inflammatory agent in cosmetics are prepared according to claim any one of 1-6.
8. application according to claim 7, it is characterised in that described cosmetics are handmade soap.
9. a kind of handmade soap with antibacterial anti-inflammatory effect, it is characterised in that described handmade soap includes following parts by weight group
Point：Antibacterial anti-inflammatory agent 3.7-8.6 parts, laurate 4.2-7.8 parts, sorb (sugar) alcohol 6.5-12 parts, palm fibre as claimed in claim 1
Palmitic acid acid 3.4-7.3 parts, sodium hydroxide 0.1-0.5 parts, coconut oil 3.5-8.0 parts, Sodium Hyaluronate 0.01-0.05 parts, glycerine
2.1-5.4 parts, PEG-7 dimethyl silicone polymer 0.6-1.7 parts, Phenoxyethanol 0.1-0.5 parts, Sensiva SC50 0.1-0.3
Part, deionized water surplus.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111803418A (en) * | 2020-07-31 | 2020-10-23 | 广州荃智美肤生物科技研究院有限公司 | Antiviral composition and application thereof in daily necessities for children |
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2017
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| CN105434239A (en) * | 2015-12-09 | 2016-03-30 | 名臣健康用品股份有限公司 | Application of skin whitening composition containing natural plant source to cosmetics |
| CN105616336A (en) * | 2015-12-14 | 2016-06-01 | 诺斯贝尔化妆品股份有限公司 | Naturally sourced composite moisturizing agent |
| CN106511254A (en) * | 2016-10-24 | 2017-03-22 | 广西智宝科技有限公司 | Shampoo for promoting hair growth and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111803418A (en) * | 2020-07-31 | 2020-10-23 | 广州荃智美肤生物科技研究院有限公司 | Antiviral composition and application thereof in daily necessities for children |
| CN111803418B (en) * | 2020-07-31 | 2023-08-04 | 广州荃智美肤生物科技研究院有限公司 | Antiviral composition and application thereof in children daily necessities |
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Application publication date: 20171219 |