CN111803418A

CN111803418A - Antiviral composition and application thereof in daily necessities for children

Info

Publication number: CN111803418A
Application number: CN202010759576.1A
Authority: CN
Inventors: 张太军; 黄劲松; 李涛; 林沛东; 胡文杰
Original assignee: Guangzhou Quanzhi Meifu Biotechnology Research Institute Co ltd
Current assignee: Guangzhou Quanzhi Meifu Biotechnology Research Institute Co ltd
Priority date: 2020-07-31
Filing date: 2020-07-31
Publication date: 2020-10-23
Anticipated expiration: 2040-07-31
Also published as: CN111803418B

Abstract

The invention discloses an antiviral composition and application thereof in daily necessities for children. The antiviral composition comprises the following raw materials in parts by weight: 5-10 parts of pomegranate bark extract, 3-15 parts of violet herb polysaccharide, 1-5 parts of black poplar extract, 2-8 parts of hypericum japonicum extract, 1-4 parts of vitamin C, 10-20 parts of glycerol and 20-40 parts of water-containing matrix. The antiviral composition has stable property, and the process for adding the antiviral composition into cosmetics is simple; and simultaneously, the composition is added into cosmetics for effect test, and the result shows that the composition has the effects of killing and inhibiting viruses. The antiviral composition can be added into washing products, particularly washing products for children, so that the children can be effectively prevented from being infected by viruses, and the body health of the children is protected.

Description

Antiviral composition and application thereof in daily necessities for children

Technical Field

The invention belongs to an antiviral composition, belongs to the technical field of daily chemical products, and particularly relates to an antiviral composition and application thereof in daily necessities for children.

Background

Harmful bacteria and viruses in the environment pose a great threat to human health. The threat of staphylococcus aureus, salmonella, escherichia coli and other traditional germs is not eliminated, and the spread of dengue fever, avian influenza, SARS, novel coronavirus and other viruses brings more serious threat to human health and causes huge economic loss and social uneasiness. A virus is a noncellular organism that is small in size, simple in structure, contains only one nucleic acid (DNA or RNA), and must be parasitic in living cells and proliferated in a replicative manner.

Conventional studies suggest that the virus does not have a complete cellular structure and can only replicate within the host cell, and thus the virus dies quickly once it leaves the host cell. However, new studies have shown that the viability of the virus is far more persistent than human imagination. Research shows that the novel coronavirus COVID-19 can survive for hours on the surface of a smooth object and can survive for 1 to 5 days in an environment with proper temperature and humidity, and the virus has persistent vitality.

Research shows that the hand washing is an important means for blocking the transmission of bacteria and viruses and preventing disease infection, so that the hand and elbow cleaning is a common habit of people, and the hand washing solution containing various bacteriostatic agents is favored by consumers along with the development of disease transmission.

The traditional bacteriostatic hand sanitizer contains chemical bacteriostatic agents: salicylic acid, parachlorometaxylene, parachlorometaxylenol and the like have good inhibition effect on hand bacteria. However, no special hand sanitizer is currently available for killing viruses. The 75% alcohol sanitizer is the most effective disinfection product and is widely used in many situations. However, 75% alcohol disinfectants can cause skin dehydration, which is not suitable for children to use for many times. However, no special hand sanitizer for children specially aiming at virus killing exists in the current market.

Therefore, developing a special antiviral liquid soap for children to fill up the blank of the current technology and market is the responsibility of the research and development technicians in the industry.

Researches show that many components in natural plants have antiviral effects, but the development of the antiviral effect is generally limited to the generation of the antiviral effect in a human body after oral administration, and related research and application are not found for viruses on the body surface (particularly hands).

Disclosure of Invention

In view of the above problems, it is an object of the present invention to provide an antiviral composition which is physically and chemically stable, and which can be added to daily necessities not only with a simple process but also effectively kill and inhibit viruses after use.

The invention also aims to provide the application of the antiviral composition in daily necessities.

The purpose of the invention is realized by the following technical scheme:

an antiviral composition comprises pomegranate rind extract, violet herb polysaccharide, black poplar extract, hypericum japonicum extract, vitamin C, glycerol and water, wherein the weight parts of the raw materials are as follows:

5-10 parts of pomegranate bark extract;

3-15 parts of violet hirsute polysaccharide;

1-5 parts of black bayberry extract;

2-8 parts of a hypericum japonicum extract;

1-4 parts of vitamin C;

10-20 parts of glycerol;

20-40 parts of a water-containing matrix.

Further, the composition comprises the following raw materials in parts by weight:

7 parts of pomegranate bark extract;

9 parts of violet hirsute polysaccharide;

2 parts of black poplar extract;

3 parts of hypericum japonicum extract;

2 parts of vitamin C;

15 parts of glycerol;

30 parts of an aqueous matrix.

Further, the pomegranate rind extract, violet herb polysaccharide, black poplar extract and hypericum japonicum extract can be obtained from corresponding extracts of water which is a commercially available extraction solvent.

Further, the aqueous base is a mixture of water and polyhydric alcohol; the polyhydric alcohol is selected from one or more of propylene glycol, butanediol, pentanediol, hexanediol, octanediol, sebacic acid diol and octanediol, and the weight ratio of the polyhydric alcohol to the water-containing matrix is 1-20%.

In addition, the invention also discloses a preparation process of the antiviral composition, which comprises the following steps:

1) dissolving vitamin C in water to obtain component A;

2) respectively dispersing pericarpium Granati extract, Viola Dolabra polysaccharide, flos populi Davidianae extract, and herba Hyperici Japonici extract in glycerol to obtain component B;

3) adding the component B into the component A, adding the aqueous matrix, and stirring for dissolving to obtain the antiviral composition.

Furthermore, the antiviral composition is applied to daily necessities for children, and the daily necessities are washing articles, in particular to hand sanitizer articles for children.

Further, the antiviral composition is applied to daily necessities for children, the antiviral composition is added into the hand sanitizer for children, the addition amount of the composition in the hand sanitizer is 0.1-8%, and the balance is conventional auxiliary materials for the hand sanitizer.

Furthermore, the antiviral composition is applied to daily necessities for children, the addition amount of the composition in the hand sanitizer is 1.0-5%, and the balance is conventional hand sanitizer auxiliary materials.

Furthermore, the antiviral composition is applied to daily necessities for children, the daily necessities are skin care products, particularly skin care products for children, the addition amount of the composition in the skin care products for children is 1.0-5%, and the balance is conventional auxiliary materials of the skin care products for children.

Further, the application of the antiviral composition in daily necessities for children is that the addition mode of the antiviral composition is as follows: adding into the final stage of the production of skin care product for children, and stirring.

The functions of the raw material components are described below.

Extract of pomegranate rind

The pericarpium Granati is dry pericarp of plant of punicalagiaceae, genus punicalagium, and small deciduous tree. Sour in nature and astringent in taste, and has effects of astringing intestine, stopping bleeding, and expelling parasites. Research shows that pomegranate rind contains tannin capable of coagulating protoplasm in microbe and thus has the effect of inhibiting and killing virus.

② Viola Douglas polysaccharide

The viola hirta is a perennial herb of the genus viola of the family violaceae, and researches show that the viola hirta polysaccharide extracted from the viola hirta has the effects of inhibiting and killing viruses. The viola hirsuta polysaccharide can directly act with viruses through negative charges carried on the surface of the viola hirsuta polysaccharide, so that the infectivity of the viruses is inhibited, and even the viruses are directly killed, so that the viola hirsuta polysaccharide loses infectivity.

③ the extract of black poplar

Myricetin (myricetin) has a chemical name of 3, 5, 7, 3', 4', 5' -hexahydroxyflavone, myricetin and myricetin, and belongs to flavonoid compounds. Myricetin is reported to have many different biological activities, such as antioxidant, antibacterial, antithrombotic, neuroprotective and anti-inflammatory effects. Myricetin can effectively inhibit SARS coronavirus helicase protein in vitro by influencing ATPase activity. Therefore, myricetin has the potential of being developed into a novel antiviral drug.

Extract of herba Hyperici Japonici

Hypericum japonicum Thunb ex Murray belongs to Guttiferae, Hypericum L, which is also called herba Hyperici Japonici, herba Adianti chinensis, herba Hyperici Erecti, herba Cardui Crispi, herba Senecionis Cannabifolii, etc. in China folk. The whole herb of the hypericum japonicum is used as a medicine and is mainly used for clearing away heat and toxic material, stopping bleeding and reducing swelling, treating acute and chronic hepatitis, gastrointestinal disorder and the like; the secondary metabolite mainly comprises phloroglucinol derivatives, flavonoids, ketones, pyrones, dipeptides, terpenes and other components, wherein most products have certain biological activity. The research shows that the hypericum japonicum extract has obvious effect of resisting hepatitis B virus (DHBV) and certain effect of inhibiting DNA replication in a virus splitting period on EB virus (EBV).

Vitamin C

Vitamin C is structurally similar to glucose and is a polyol. In humans, vitamin C is a highly potent antioxidant used to reduce the oxidative stress of ascorbate peroxidase. An in vitro test study published in the journal of tuberculosis and respiration of china shows that vitamin C kills viruses with a dose-dependent effect: 2.5mmol/L vitamin C can kill about 90% of influenza virus, and 20mmol/L vitamin C can kill all viruses.

The invention achieves the synergistic effect by the specific combination of different components, the anti-virus composition has stable physical and chemical properties, has simple process when being added into cosmetics, and can fully play the inhibiting and killing effects of each component on viruses to achieve the aim of the invention.

By means of the technical scheme, the invention has the following advantages and beneficial effects:

1) in the research on the in vitro antiviral efficacy of the natural plant extract, the inventors surprisingly found that: part of natural plant extracts have obvious in-vitro antiviral efficacy, and the specific combination of different components can greatly enhance the killing and inhibiting effects on viruses due to the synergistic interaction between the different components.

2) The antiviral composition is applied to the hand sanitizer special for children, and after the composition is used, the hand sanitizer not only has the function of cleaning hand dirt, but also can effectively kill and inhibit viruses on hands, and can protect the health of the children.

3) The composition has stable property, is simple in process when being added into skin care products, and is suitable for industrial production. The composition disclosed by the invention is added into cosmetics, particularly hand sanitizer for children, and after the composition is used, hand viruses can be effectively killed, children are prevented from being infected by the viruses, and the health of the children is protected.

Detailed Description

The present invention will be described in more detail below with reference to specific preferred embodiments and examples of effects, but the present invention is not limited to the following examples.

The following examples 1 to 3 are process examples of preparing the antiviral composition of the present invention, respectively. The following parts by weight may be in units of g or kg.

Example 1 preparation of antiviral composition

The raw materials of the antiviral composition comprise pomegranate rind extract, violet herb polysaccharide, black poplar extract, hypericum japonicum extract and vitamin C, wherein the weight parts of the raw materials are as follows:

7 parts of pomegranate bark extract;

9 parts of violet hirsute polysaccharide;

2 parts of black poplar extract;

3 parts of hypericum japonicum extract;

2 parts of vitamin C;

15 parts of glycerol;

30 parts of a water-containing matrix (comprising water, pentanediol and hexanediol, wherein the mass ratio of the pentanediol to the hexanediol is 1:1, and the ratio of the pentanediol to the hexanediol is 6%);

the preparation process comprises the following steps:

1) dissolving vitamin C in water to obtain component A;

Example 2 preparation of an antiviral composition

The raw materials of the antiviral composition comprise pomegranate rind extract, violet herb polysaccharide, black poplar extract, hypericum japonicum extract and vitamin C, and the weight parts of the raw materials are as follows:

5 parts of pomegranate bark extract;

3 parts of violet hirsute polysaccharide;

1 part of black bayberry extract;

2 parts of hypericum japonicum extract;

1 parts of vitamin C;

10 parts of glycerol;

20 parts of an aqueous matrix (comprising water and caprylyl glycol, wherein the mass percentage of the caprylyl glycol is 10%);

the preparation process comprises the following steps:

1) dissolving vitamin C in water to obtain component A;

Example 3 preparation of an antiviral composition

10 parts of pomegranate bark extract;

15 parts of violet hirsute polysaccharide;

5 parts of black poplar extract;

8 parts of hypericum japonicum extract;

4 parts of vitamin C;

20 parts of glycerol;

40 parts of a water-containing matrix (comprising water, pentanediol, butanediol and hexanediol, wherein the mass ratio of the pentanediol to the butanediol to the hexanediol is 2:1:1, and the weight ratio of the pentanediol to the butanediol to the hexanediol is 5%);

the preparation process comprises the following steps:

1) dissolving vitamin C in water to obtain component A;

Example 4 preparation of hand sanitizer for children

The antiviral composition in the example 1 is added into daily necessities to prepare the hand sanitizer for children, and the hand sanitizer comprises the following raw material components in percentage by weight:

phase A:

40% of water;

sodium laureth sulfate 8.0%;

2.0% of sodium methyl cocoyl taurate;

EDTA-2Na 0.05％；

the balance of ionized water;

phase B:

3.0% of the antiviral composition of example 1;

the balance of deionized water.

The preparation method comprises the following steps:

1) stirring the phase A, heating to 83 ℃, and keeping the temperature and stirring for 30 minutes;

2) adding phase B, and stirring for 30min to obtain the final product.

Example 5 preparation of hand sanitizer for children

The antiviral composition in the example 2 is added into daily necessities to prepare the hand sanitizer for children, and the hand sanitizer comprises the following raw material components in percentage by weight:

phase A:

40% of water;

10.0 percent of sodium laureth sulfate;

1.0% of sodium lauroyl sarcosinate;

EDTA-2Na 0.05％；

the balance of ionized water;

phase B:

2.5% of the antiviral composition of example 2;

the balance of deionized water.

The preparation method comprises the following steps:

2) adding phase B, and stirring for 30min to obtain the final product.

Example 6 (preparation of hand sanitizer for children)

The antiviral composition in the example 3 is added into daily necessities to prepare the hand sanitizer for children, and the hand sanitizer comprises the following raw material components in percentage by weight:

phase A:

40% of water;

9.0 percent of sodium laureth sulfate;

1.5 percent of sodium lauroyl glutamate;

EDTA-2Na 0.05％；

the balance of ionized water;

phase B:

2.8% of the antiviral composition of example 3;

the balance of deionized water.

The preparation method comprises the following steps:

2) adding phase B, and stirring for 30min to obtain the final product.

The following are contents of the effect test examples.

Effect test example 1

1. In vitro antiviral experiments:

1) preparing a sample solution to be detected:

accurately weighing the sterilized sample, dissolving the sample by using a cell maintenance solution, fixing the volume to 10mL, then diluting the sample by using the cell maintenance solution in a multiplying ratio, and finally, wherein the concentration of the sample solution to be detected is 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125 and 0.0625 mg/mL. The groups are shown in table 1 below:

TABLE 1 samples to be tested

2) Determination of direct killing effect of sample diluent on H9N2 avian influenza virus

The virus titer was 100TCID₅₀(half the amount of tissue cells infected) virus fluid and less than half the Effective Concentration (EC)₅₀) The sample diluent to be tested is mixed in equal volume, and placed at 37 ℃ and 5% CO₂The culture was performed for 2 hours in a cell culture chamber, and the mixture was added to a 96-well cell culture plate containing a single layer of MDCK (canine kidney cells); DMEM added to the cell control group; virus control group plus 100TCID₅₀And (4) virus liquid. Each group was replicated in 8 wells, and the amount of solution added was 0.1 mL/well. Placing at 37 ℃ and 5% CO₂Culturing in incubator, observing cell CPE (cytopathic effect) every 12h under microscope, recording CPE condition of each well when virus control group CPE reaches 90%, determining OD value at 490nm wavelength by enzyme labeling instrument using MTT method, and calculating EC by Reed-Muench formula₅₀Therapeutic Index (TI):

EC₅₀either [ Antilog (value above 50% CPE percent sample dilution-specific distance)]×C；

Therapeutic Index (TI) ═ TC₅₀/EC₅₀；

Wherein C is the final concentration of the drug in the first well, TC₅₀At half toxic concentration.

The results are shown in Table 2 below.

TABLE 2 TI test results of H9N2 avian influenza Virus killing effect of diluent

Group of	A	B	C	D	F	G	H	I	J	K	L	M
													TI	－	11.67	10.59	9.31	8.84	14.22	13.17	12.89	15.56	16.74	18.26	30.31

Note: -means no TI.

As can be seen from the results in table 2, example 2 containing the composition of the present invention has stronger direct virus killing effect (i.e. higher therapeutic index TI) compared with a single component or different combinations of other components under the same conditions, which indicates that the composition of the present invention can achieve synergistic effect to achieve the purpose of the present invention.

Effect test example 2

2. Skin safety test

The skin irritation degree was evaluated by diluting 20 male and female children aged 6 to 12 years old with the composition of any of examples 1 to 3 20 times (equivalent to 5% of the formulation) in water to prepare a solvent for experiment.

The test method comprises the following steps: after applying 10. mu.L of the test solution to the front side of the arm of the subject, the test part was closed and covered for 24 hours, and then the skin reactions (with pure water as a blank control) were examined after 30 minutes and 24 hours after removing the cover according to the nomenclature of the CTFA guidelines.

According to a judgment reference: the scores obtained for the subjects were averaged, and the evaluation results were low irritation if the score was less than 1, slight irritation if the score was less than 3, moderate irritation if the score was less than 5, and strong irritation if the score was 5 or more.

Table 3 skin safety test results

Experimental group	Examples	Blank control
			Skin irritation index (PII)	0.13	0.11

As can be seen from the data in Table 3 above, the skin irritation results of the examples are less than 1, and the irritation is close to that of pure water, and it can be confirmed that the antiviral composition of the present invention is low in irritation level and safe for children's skin.

The foregoing is a more detailed description of the invention, taken in conjunction with specific preferred embodiments thereof, and it is not intended that the invention be limited to the specific details set forth herein. It will be understood by those skilled in the art that various alternatives and modifications may be made to the described embodiments without departing from the spirit of the invention and these alternatives and modifications are to be considered as within the scope of the invention.

Claims

1. An antiviral composition characterized by: the composition comprises a pomegranate rind extract, violet dauricola polysaccharide, a black poplar extract, a hypericum japonicum extract, vitamin C, glycerol and water, wherein the weight parts of the raw materials are as follows:

5-10 parts of pomegranate bark extract;

3-15 parts of violet hirsute polysaccharide;

1-5 parts of black bayberry extract;

2-8 parts of a hypericum japonicum extract;

1-4 parts of vitamin C;

10-20 parts of glycerol;

20-40 parts of a water-containing matrix.

2. The antiviral composition of claim 1, wherein: the weight parts of the raw materials are as follows:

7 parts of pomegranate bark extract;

9 parts of violet hirsute polysaccharide;

2 parts of black poplar extract;

3 parts of hypericum japonicum extract;

2 parts of vitamin C;

15 parts of glycerol;

30 parts of an aqueous matrix.

3. The antiviral composition of claim 1, wherein: the pericarpium Granati extract, Viola Dolabra polysaccharide, flos populi Tomentosae extract, and herba Hyperici Japonici extract can be obtained from corresponding commercially available extracts with water as extraction solvent; the vitamin C is derived from extract.

4. The antiviral composition of claim 1, wherein said antiviral composition is prepared by the process of:

1) dissolving vitamin C in water to obtain component A;

5. The antiviral composition of claim 1, wherein: the aqueous matrix is a mixture of water and polyhydric alcohol; the polyhydric alcohol is selected from one or more of propylene glycol, butanediol, pentanediol, hexanediol, octanediol, sebacic acid diol and octanediol, and the weight ratio of the polyhydric alcohol to the water-containing matrix is 1-20%.

6. Use of an antiviral composition according to any of claims 1 to 4 in a commodity, characterized in that: the daily necessities are washing articles, in particular to hand sanitizer articles for children.

7. Use of an antiviral composition according to claim 5 in a daily product, wherein: the antiviral composition is added into the hand sanitizer for children, the addition amount of the composition in the hand sanitizer is 0.1-8%, and the balance is conventional auxiliary materials of the hand sanitizer.

8. Use of an antiviral composition according to claim 6 in a daily product, wherein: the addition amount of the composition in the hand sanitizer is 1.0-5%, and the balance is conventional hand sanitizer auxiliary materials.

9. Use of an antiviral composition according to any of claims 1 to 4 in a commodity, characterized in that: the daily necessities are skin care products, particularly skin care products for children, the addition amount of the composition in the skin care products for children is 1.0-5%, and the balance is conventional auxiliary materials of the skin care products for children.

10. The use of an antiviral composition as claimed in claim 8 in a daily product for children, wherein: the addition mode of the antiviral composition is as follows: adding into the final stage of the production of skin care product for children, and stirring.