CN109745514A - A kind of composition and its preparation method and application with anti-bacteria and anti-virus - Google Patents
A kind of composition and its preparation method and application with anti-bacteria and anti-virus Download PDFInfo
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Abstract
The invention discloses a kind of compositions with anti-bacteria and anti-virus, while also disclosing the preparation method and purposes of the composition.The composition in Ginger P.E, Fructus Chebulae extract, Herba Agrimoniae extract, nutgall extractive, Cacumen Securinegae Suffruticosae extract, Rhizoma Dryopteris Crassirhizomatis extract, pokeberry extract, Flos Caryophylli extract, cinnamomum cassia extract, Flos Chrysanthemi Indici extract, folium eucalypti extract, forsythia suspense extraction, Radix Stemonae extract, Geranium extract, atractylodes chinensis, Folium Artemisiae Argyi extract, Herba Lysimachiae foenumgraeci extract, Radix agastaches extract, Turmeric P.E, mint extract by choosing any one kind of them or more than one are formed.Synergistic function is good between each ingredient of the present composition, have effects that significantly to dispel to various pathogenic bacteria existing for skin, air etc. and virus, and without any stimulation and side effect, while each ingredient origin is easy to get, it is simple to prepare composition process, it is suitable for mass production.
Description
Technical field
The invention belongs to drug, disinfectant, cosmetics, health treatment or hygiene article sector, in particular to one kind has
Composition of anti-bacteria and anti-virus and its preparation method and application.
Background technique
Pathogenic microorganism, which refers to, can invade human body, cause the infection even microorganism of infectious disease or pathogen.Cause of disease
It is maximum with the harmfulness of bacterium and virus in body.After pathogen invades human body, it is disease that human body, which is exactly the place of pathogen existence,
The host of substance.Pathogen carries out the pathology that growth and breeding, release toxicant etc. cause body different degrees of in host and becomes
Change, this process is known as infecting.It, can the generation of exciting human immune system while infecting after pathogen invades human body
A series of immune responses are fought therewith, this is referred to as immune.
Infecting and being immunized is conflict, if host is strong enough, infection can not be formed, even if foring sense
Dye, pathogen are also most likely gradually withered away, then Rehabilitation；If host is very weak and pathogen is very violent, expansion is infected
It dissipates, patient will be dead.
When fighting pathogenic infection, in addition to the strength of host itself, effective antibacterial and antiviral drug and some other
Safeguard procedures are also essential, for example, carry out disinfection processing to external environment, human skin etc., it is anti-using some antibacterials
Viral amenities etc., comprehensive cutting infect pathogeny.
For many years, people position quasi- relatively approval, but its side effect is more, causes drug resistance strong to the rapid-action of chemical drug
It is still incomparable with traditional Chinese medicine etc. all various aspects.Equally, the disinfectant of present commercial type is also chemical component mostly,
Its inhibitory anti-virus ability is stronger, but if improper use, can also cause damages to human health: for example to skin, viscous
Film and the upper respiratory tract have intense stimulus effect；It can cause larynx, bronchial inflammation, oedema, spasm after sucking, chemical pneumonia,
Pulmonary edema；It can cause burn feeling after contact, cough, wheeze, laryngitis, shortness of breath, headache, nausea and vomiting；It can also be generated simultaneously
It is dangerous: for example explodes, pollution environment etc..Chemical classes amenities is used for a long time in people, can also cause to human body more or less
Damage.
Compared to the drug, disinfectant and amenities of chemical component, the product of medicinal herb components is more next in today's world
More it is taken seriously, it is cheap and easy to get, safe and non-toxic quite to be praised highly by people, therefore develop a kind of drug of anti-bacteria and anti-virus, disinfectant
Or amenities be people there is an urgent need to.
Summary of the invention
Based on the above status, an object of the present invention is to provide a kind of composition with anti-bacteria and anti-virus, Chinese herbal medicine
Ingredient, good effect, safety are without side-effects.
It is a further object of the present invention to provide the methods for preparing above-mentioned composition.
It is yet another object of the invention to provide the purposes of above-mentioned composition.
To realize above scheme, the present invention provides following technical schemes:
The composition is mentioned by Ginger P.E, Fructus Chebulae extract, Herba Agrimoniae extract, nutgall extractive, Common Leafflower Herb
Take object, Rhizoma Dryopteris Crassirhizomatis extract, pokeberry extract, Flos Caryophylli extract, cinnamomum cassia extract, Flos Chrysanthemi Indici extract, folium eucalypti extract,
Forsythia suspense extraction, Radix Stemonae extract, Geranium extract, atractylodes chinensis, Folium Artemisiae Argyi extract, Herba Lysimachiae foenumgraeci extract, Pogostemon cablin
Extract, Turmeric P.E are chosen any one kind of them in mint extract or more than one compositions.
Further, the composition is extracted by ginger extract, hairyvein agrimony extracting solution, Common Leafflower Herb extracting solution, thick wood-fern rhizome
Liquid, Phytolacca acinosa extracting solution, cortex cinnamomi extracting solution, wild chrysanthemum extracting solution, folium eucalypti extracting solution, tuber of stemona extracting solution, geranium wilfordii extracting solution, rhizoma atractylodis
Oil, Blumea oil, cinnamon oil, lavender oil, mother chrysanthemum caul-fat, patchouli oil, eucalyptus oil, curcuma oil, choose any one kind of them in peppermint oil or
More than one compositions.
Further, the composition by ginger extract, hairyvein agrimony extracting solution, thick wood-fern rhizome extracting solution, cortex cinnamomi extracting solution,
Wild chrysanthemum extracting solution, folium eucalypti extracting solution, tuber of stemona extracting solution, geranium wilfordii extracting solution, Blumea oil, cinnamon oil, lavender oil, folium eucalypti
Oil, curcuma oil are chosen any one kind of them in peppermint oil or more than one compositions.
Further, the composition is made of or thick wood-fern rhizome extracting solution, eucalyptus oil, mother chrysanthemum caul-fat, peppermint oil by cortex cinnamomi
Extracting solution, cinnamon oil, eucalyptus oil, peppermint oil composition are made of Phytolacca acinosa extracting solution, eucalyptus oil, mother chrysanthemum caul-fat, cinnamon oil.
Further, the composition is formed by following weight: 0.01-50 parts of thick wood-fern rhizome extracting solution, eucalyptus oil
0.01-50 parts, 0.01-50 parts of mother chrysanthemum caul-fat, 0.001-20 parts of peppermint oil or
0.1-100 parts of cortex cinnamomi extracting solution, 0.001-50 parts of cinnamon oil, 0.001-50 parts of eucalyptus oil, peppermint oil 0.001-20
Part or
0.01-50 parts of Phytolacca acinosa extracting solution, 0.001-20 parts of eucalyptus oil, 0.001-20 parts of mother chrysanthemum caul-fat, cinnamon oil 0.001-
10 parts.
Above formulation dosage inhibition zone diameter in anti-microbial property detection is greater than 7mm, shows there is bacteriostasis；Antiviral property
In capable of detecting, SI > 1 embodies effective antiviral functions.
Further, the composition is formed by following weight: 0.3-20 parts of thick wood-fern rhizome extracting solution, eucalyptus oil
0.3-20 parts, 0.3-20 parts of mother chrysanthemum caul-fat, 0.1-10 parts of peppermint oil or
1-50 parts of cortex cinnamomi extracting solution, 0.01-20 parts of cinnamon oil, 0.01-30 parts of eucalyptus oil, 0.005-10 parts of peppermint oil or
0.2-20 parts of Phytolacca acinosa extracting solution, 0.1-10 parts of eucalyptus oil, 0.1-10 parts of mother chrysanthemum caul-fat, 0.01-5 parts of cinnamon oil.
Formula after advanced optimizing is compared with the formula before optimizing to the anti-microbial property of staphylococcus aureus and Escherichia coli
More preferably effect is realized in detection, inhibition zone diameter is up to 14-15mm；Avian influenza virus H7N3 and H9N2 antiviral property is examined
More preferably effect is equally realized in survey, SI is up to 6.5-7.5, therefore preferably rear each recipe ingredient dosage compatibility is more precise and appropriate, skill
Art effect is more excellent, and formula drug effect can be played to ultimate attainment, marrow embodiment cause to the greatest extent.
Above-mentioned composition ingredient origin is as follows:
Ginger has stomach invigorating, anti-gastric-ulcer, cholagogue, liver protection, heart tonifying, inhibits platelet aggregation, anti-corona, antitumor, maincenter
Inhibition, strengthen immunity, antibacterial, desinsection, anti-inflammatory effect.
Myrobalan has resisting pathogenic microbes effect.In vitro test shows: myrobalan's water decoction (100%) is to various dysentery bars
Bacterium has inhibiting effect, stronger to the effect of Pseudomonas aeruginosa, corynebacterium diphtheriae, to staphylococcus aureus, Escherichia coli, pneumonia ball
Bacterium, hemolytic streptococcus, proteus, bacillus typhi murium also have inhibiting effect (flat band method).In addition, myrobalan is in vitro to typhoid fever
Bacillus also has good antibacterial action
Hairyvein agrimony has the effects that hemostasis, antimicrobial antiphlogistic, antiviral, prevention Leptospira, expelling parasite, antitumor, also has
There is the effects for the treatment of Meniere's disease, diabetes.
Chinese gall, Chinese gall antibiotic effective ingredient containing there are many, is mainly distributed on skin zone.Chinese gall decoction is to being inoculated in chicken
The influenza PR8 strain of influenza virus type A virus of embryo has inhibiting effect.100% decoction is burrowed method with plate, to Pseudomonas aeruginosa, shigella dysenteriae, change
Shape bacillus, Escherichia coli, typhoid bacillus, bacillus enteritidis, bacillus anthracis, corynebacterium diphtheriae, staphylococcus aureus, beta streptococcus
And pneumococcus has inhibiting effect, the mechanism of action is that have freezing action to albumen because of it.
Thick wood-fern rhizome has anthelmintic action；There is different degrees of bactericidal effect to a variety of bacterium, to Gram-positive and feminine gender disease
Poison all has inhibition and killing effect.Thick wood-fern rhizome contains polyphenols, and polyphenols can inhibit the absorption of virus to make
With and virus protein and RNA synthesis.There is document report to carry out the screening of extracorporeal antivirus effect Chinese herbal medicine, hair using chicken embryo test method(s)
Existing rhizome of cyrtomium has preferable inactivation AIV effect in vitro.
Common Leafflower Herb, reducing fever and causing diuresis, improving eyesight, disperse accumulation.For nephritic dropsy, urinary infection, calculus, enteritis, dysentery, children
Infantile malnutrition due to digestive disturbances or intestinalparasites, eye keratitis, icteric hepatitis；External application controls green bamboo snake and bites.
Phytolacca acinosa, water decoction, tincture have inhibiting effect to Bacillus influenzae, a variety of shigella dysenteriaes, pneumococcus；To dermatophyte
Also there is inhibiting effect.
Cloves, in vitro test prove that the alcohol extract of cloves is to corynebacterium diphtheriae, bacillus anthracis, paratyphosum Bacterium, dysentery bar
Bacterium, staphylococcus aureus, staphylococcus albus, comma bacillus have inhibiting effect.In addition, in vitro test proves, cloves pair
Influenza virus PR8 strain virus also has inhibiting effect.
Cortex cinnamomi, cassia oil have powerful bactericidal effect, better than negative patient to the effect of G+ bacteria.Cinnamon oil
Main component is cinnamic acid, it is existing research have shown that cinnamic acid to Escherichia coli, staphylococcus aureus, salmonella,
Hay bacillus, proteus etc. all have significant bactericidal effect.
Wild chrysanthemum has stronger anti-influenza virus activity.Test proves that wild chrysanthemum water extract antiviral activity is very high,
It can inhibit the activity of influenza virus, clinically commonly use its prophylactic agent as influenza virus.Flos Chrysanthemi Indici volatile oil is to golden yellow
Staphylococcus effect is stronger, effective to staphylococcus albus.Wild chrysanthemum water extract is made with good inside and outside resisiting influenza virus
With, and have good adjustment effect to the cell immune system of influenza a virus infection mouse.Wild chrysanthemum mainly passes through disease-resistant
Poison absorption, antiviral duplication, these three modes of action of direct inactivation of viruses inhibit virus, protect cell.
Folium eucalypti, folium eucalypti decoction is in vitro to staphylococcus aureus, hemolytic streptococcus, dysentery with bloody stool bacillus, typhoid bacillus, big
Enterobacteria, Pseudomonas aeruginosa etc. have inhibiting effect.There are also inhibit staphylococcus aureus and paratyphoid B bar for folium eucalypti Aqueous extracts
The oxygen consumption of bacterium and its effect of succinate dehydrogenase activity.Studies have reported that the main composition 1, 8-Cineole in eucalyptus oil
It can prevent avian infectious bronchitis virus (Avian Infectious Bronchitis virus, IBV) duplication and group
Dress inhibits IBV effect to reach.
Fructus Forsythiae, weeping forsythia concentrated decoction have antibacterial action in vitro, can inhibit typhoid bacillus, paratyphosum Bacterium, Escherichia coli,
Shigella dysenteriae, corynebacterium diphtheriae and comma bacillus, staphylococcus, streptococcus etc..Fructus Forsythiae herb extract-hypericin, to disease
Poison has conspicuousness killing effect, especially avian influenza virus.Its Antiviral Mechanism mainly passes through the assembly and release of viral interference
The integration of disease is influenced simultaneously, to kill virus.
The tuber of stemona, bactericidal effect is most one of outstanding in many Chinese medicines, its not only pneumococcus and hammer common to body lung
The good killing effect of bacterium, in human body meningitis bacterium and shigella dysenteriae also good killing effect.In addition the tuber of stemona is anti-
It is viral also especially strong, it is the most obvious especially for the resistance of some influenza virus, it can be imitated after people are edible
Prevent the generation of virus flu.
Geranium wilfordii has antibacterial, antiviral, antiulcer and anti-diarrhea effect.
Rhizoma atractylodis, have effects that it is hypoglycemic, treatment abdominal distension gas, stomach invigorating, diuresis, anticancer, eliminating the phlegm, hypnosis, sterilization the effects of and.
Folium artemisiae argyi is a kind of drug of broad-spectrum antibacterial antiviral, it has inhibition and lethal effect to a lot of viruses and bacterium,
There is certain preventive and therapeutic effect to respiratory disease.
Lavender has antiviral, preservation antioxidation, disinfection and sterilization, tranquilizing effect, treats inflammation, scald, burning
Wound, ulcer effect are significant, thus in terms of being widely used in health care.
Pogostemon cablin, whet the appetite preventing or arresting vomiting, delivers relieving summer-heat, also has resisiting influenza virus, Respiratory Syncytial Virus(RSV), adenovirus, Ke Sa
The effect of odd virus and herpes simplex virus.
Turmeric has the effects that good anti-oxidant, antibacterial, anti AIDS virus, antitumor, is clinically used Chinese medicine.
Peppermint has significant antiviral and bactericidal effect.Experiment in vitro show peppermint decoction to staphylococcus aureus,
It is staphylococcus albus, alpha streptococcus, beta streptococcus, micrococcus catarrhalis, enteritis coccus, shigella flexneri, bacillus anthracis, white
Larynx bacillus, typhoid bacillus, Pseudomonas aeruginosa, Escherichia coli, proteus etc. have antibacterial action.Studies have reported that peppermint polysaccharide exists
Also there is to Respiratory Syncytial Virus(RSV) stronger inhibiting effect in vitro.Peppermint water decoction (1:20) is in vitro to orphan virus
(ECHO11) there is inhibiting effect.
Component any combination selected by inventive formulation has anti-bacteria and anti-virus function, especially thick wood-fern rhizome extracting solution, folium eucalypti
Oil, mother chrysanthemum caul-fat, mint oil combination；Cortex cinnamomi oil extract, cinnamon oil, eucalyptus oil, mint oil combination；Phytolacca acinosa extracting solution, folium eucalypti
Oil, the combination of mother chrysanthemum caul-fat, cinnamon oil can be such that drug effect plays ultimate attainment by weight composition, and function embodiment is best.
The mechanism of action of the main component cinnamic acid restraining and sterilizing bacteria of cortex cinnamomi is mainly that there are one and benzene in cinnamic acid molecule
The aldehyde radical of ring conjugation, aldehyde radical is a nucleophilic group, is easily adsorbed by the hydrophilic group of bacterium surface and penetrates cell wall, is destroyed thin
Bacterium, fungi cell wall polysaccharide structure to playing antibacterial or bactericidal effect.In addition the aldehyde radical in cinnamic acid can in bacterium
Albumen and enzyme effect destroy the normal physiological metabolism of bacterium, play antibacterial or bactericidal effect.Polyphenols chemical combination in cortex cinnamomi
Object can have anti-avian influenza virus effect by inactivated avian influenza virus.Saponin constituent in Phytolacca acinosa, it may be possible to pass through reduction
The stability of bacterial biof iotalm, to disturb the enzymatic reaction of energetic supersession and generate fungistatic effect；Flavones in wild chrysanthemum
Constituents have the function of inhibition bacterium, can also pass through absorption, duplication, directly inactivate sick these three modes of action inhibition disease
Poison protects cell；Thick wood-fern rhizome contains polyphenols, can lead to micro- life by the integrality of destruction cell wall and cell membrane
Object cell discharges ingredient intracellular and causes the dysfunctions such as electron transmission, nutrient absorption, nucleotide synthesis and the ATP activity of film,
To inhibit the growth of microorganism；Inhibit the suction-operated of virus and the synthesis of virus protein and RNA.Eucalyptus oil and peppermint oil are logical
Inhibition bacterial activity is crossed to reach bacteriostasis；Main composition 1, 8-Cineole in eucalyptus oil can prevent virus replication
And assembling, to reach inhibition virus function；Peppermint polysaccharide also has stronger inhibiting effect to Respiratory Syncytial Virus(RSV) in vitro.
Peppermint water decoction (1:20) is in vitro to orphan virus (ECHO11) there is inhibiting effect.
As it can be seen that the above raw material be to the blocking approach of bacterium and virus it is various, can anti-bacteria and anti-virus to some extent, reach
Polytropism, multiple target point inhibition are carried out to bacterium, virus, to be effectively protected cell.
According to a preferred embodiment of the invention, the composition is exterior-applied formulation, is rendered as solution, pulvis, washes
Agent, tincture, gelling agent, emulsion, finish, ointment, paste, plaster, liniment, plastics, aerosol any one form, it is preferably molten
Liquid.
These exterior-applied formulations described above can contain pharmaceutically acceptable auxiliary material, including common acceptable oil base
Matter, aqueous matrix, preservative, antioxidant, moisturizer, skin penetration enhancer, skin care agent, thickener, solubilizer, pH tune
Save agent, emulsifier, aromatic etc..
Its common auxiliary material such as refer to tween, sapn, Cremophor RH40, PEG-60 rilanit special, ceteth,
PEG-75 stearate, cetostearyl alcohol, stereth, stearine, ceteareth, laureth sulphur
Base succinate disodium, carbomer, xanthan gum, hypromellose, hydroxyethyl cellulose, polyisobutene, magnesium sulfate, propylene
It is sour (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer, sodium acrylate/sodium acryloyldimethyl taurate copolymers, sweet
Oil, propylene glycol, butanediol, dipropylene glycol, Sodium Hyaluronate, sand ground Verbena officinalis, silk propylhomoserin, polyglycerolmethacrylate,
Wild soybean albumen, hydrolysis rice bran protein, polyethylene glycol, hexylene glycol, xylitol, polypropylene glycol, D-sorbite, amino acid, lactic acid
Sodium, urea, aloe, marine algae extract, chitin, triethanolamine, triethylamine, diethylamine, lauryl amine, sodium hydroxide, hydroxide
It is potassium, sodium bicarbonate, lactic acid, sodium lactate, Jojoba wax PEG-120 esters, allantoin, dipotassium glycyrrhizinate, bulk kelp extract, pungent
Acid/Triglyceride DDD, glycerol three (thylhexoic acid) ester, dimethyl silicone polymer, wheat-germ oil, methyl hydroxybenzoate, oxybenzene second
Ester, Nipasol, butyl hydroxybenzoate, Chlorphenesin, o- cymene -5- alcohol, vitamin E, vitamin C, glutathione, lavender essence
Oil, rose ethereal oil, Lignum Aquilariae Resinatum extract, lemon oil, polyvinylpyrrolidone, polyacrylate, Sodium Polyacrylate, vinyl alcohol,
Polyethylene, acetylation monoglyceride, wool grease, rilanit special, palmityl alcohol.
Another object according to the present invention provides the method for preparing the composition, specifically:
1) weighing raw material by formula ratio adds the solvent of various concentration individually to extract or mix extraction extraction, and extracting solution is concentrated to give
Crude extract；Or further use water extraction and alcohol precipitation method, organic solvent extractionprocess, column chromatography, carbon dioxide supercritical extraction method, water
The one or more of steam distillation method are used in combination after progress suitably refines and obtain extract；Above-mentioned crude extract or extract are original
Expect extract；
2) weigh raw extract by formula ratio, add or be not added auxiliary material to get.
Further, the method for preparing the solution, specifically:
1) raw material is weighed, water mentions, and concentration is centrifuged to obtain supernatant A, for use；
2) supernatant A is slowly added to ethyl alcohol, reaches corresponding determining alcohol, carries out alcohol precipitation, is centrifuged to obtain supernatant B, recycles ethyl alcohol,
It is concentrated to give raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and moisturizer, thickener, purifying is added in filtering, filtrate
Water heating, heat preservation obtain A phase；
4) weighing feedstock oil by formula ratio adds emulsifier to heat, and stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, several minutes of homogeneous, continue to stir, slow cooling to obtain the final product.
Further, the moisturizer is glycerol, propylene glycol, 1,3-BDO, dipropylene glycol, sorb (sugar) pure and mild sorbierite
It is polyethers -20, amides moisturizer, glycerin polyether class moisturizer, lactic acid and sodium lactate, pyrrolidone sodium carboxylate, ethoxy urea, red
Moss sugar alcohol, Sodium Hyaluronate, carbohydrate isomer, D-pantothenyl aleohol, polyethylene glycol, avenabeta glucosan, trehalose, allantoin,
Silk propylhomoserin, hydrolytic soya bean protein, hydrolysis rice bran protein, hexylene glycol, xylitol, polypropylene glycol, chitin, one in glycine betaine
Kind or more than one；The thickener is carbomer, xanthan gum, Arabic gum, bassora gum, locust bean gum, carboxymethyl cellulose
Sodium, hypromellose, methylcellulose, hydroxyethyl cellulose, acrylic acid (ester) analog copolymer, glycerol polymethylacrylic acid
Ester polymer, polyvinyl alcohol, ammonium acryloyl taurate/VP copolymer, hydroxy-ethyl acrylate/acryloyldimethyl ox sulphur
One of sour sodium copolymer, polyisobutene, magnesium sulfate, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer
Or more than one；The emulsifier is methyl sesquistearate, PEG-20 methyl glucoside sesquialter hard acid ester, tristearin
Alcohol polyethers -2, stereth -21, cetearyl alcohol alcohol ether -2, cetearyl alcohol alcohol ether -21, docosanol ether -25, glyceryl stearate
Acid esters, Tween-80, Tween-60, Tween-20, cetearyl glucoside/cetostearyl alcohol, stearine, C20-C22
Alkylol/C20 alkyl glucoside, stearoyl-glutamic acid sodium, Sucrose Polystearic Acid Esters/Parleam, -3/ spermaceti of polyglycereol
It is stearyl alcohol olive oleate, acetylation monoglyceride, wool grease, rilanit special, one or more kinds of in palmityl alcohol.
Further, the method for the composition is prepared are as follows:
1) raw material is weighed, the water that 9-11 times of medicinal material amount is added extracts 1-4 times, each 1-3h；It filters (200 mesh), merges filter
Liquid, being concentrated under reduced pressure into relative density is 1.05~1.10 (60 DEG C)；Centrifuged supernatant A is obtained after centrifugation, for use；
2) supernatant A is slowly added to ethyl alcohol, and determining alcohol is made to reach 60-80%, carries out alcohol precipitation；Centrifugation obtains supernatant B, returns
Ethyl alcohol is received, being concentrated under reduced pressure into relative density is 1.05~1.10 (60 DEG C), obtains raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and filtering, filtrate adds moisturizer, thickener, purified water
65 DEG C -85 DEG C are heated to, is stirred evenly, heat preservation 15min obtains A phase；
4) weighing feedstock oil by formula ratio adds emulsifier to be heated to 65 DEG C -80 DEG C, stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, homogeneous 5 minutes, continue to stir 20min, slow cooling to 40-
60 DEG C to get.
A further object according to the present invention provides the composition or the use of composition that the preparation method is prepared
On the way, the composition is for preparing the purposes in drug, disinfectant, cosmetics, health treatment or amenities.
Further, purposes of the composition in the skin sanitary article or air sanitizer for preparing anti-bacteria and anti-virus.
Each raw extract can be made by oneself also commercially available and obtain in the present invention.
Beneficial effects of the present invention
Each ingredient of the present composition, when individualism, anti-bacteria and anti-virus function is all relatively low or relatively simple, still
Inventor compound and then is gradually sieved in conjunction with modern medicine study according to traditional Chinese medical theory basis and the mechanism of action
Choosing, is verified optimum organization repeatedly, keeps synergistic function performance between each ingredient ultimate attainment, to various existing for skin, air etc.
Pathogenic bacteria and virus, which play, significantly dispels effect, and without any stimulation and side effect, while each ingredient origin is easy to get, preparation group
Object simple process is closed, it is suitable for mass production.
Specific embodiment
Foregoing invention content of the invention is described in further detail With reference to embodiment.
But the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to following embodiments.On not departing from the present invention
In the case of stating technical idea, according to ordinary skill knowledge and customary means, various replacements and change are made, should all include
Within the scope of the invention.
6 parts of 1 cortex cinnamomi extracting solution of embodiment, 0.24 part of cinnamon oil, 0.1 part of eucalyptus oil, 0.01 part of peppermint oil
1) raw material is weighed, the water that 9 times of medicinal material amounts are added extracts 2 times, each 1h；It filters (200 mesh), merging filtrate, decompression
Being concentrated into relative density is 1.05~1.10；Centrifuged supernatant A is obtained after centrifugation, for use；
2) supernatant A is slowly added to ethyl alcohol, and determining alcohol is made to reach 70%, carries out alcohol precipitation；Centrifugation obtains supernatant B, recycles
Ethyl alcohol is concentrated under reduced pressure into relative density 1.05~1.10, obtains raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and filtering, filtrate adds xanthan gum, glycerol, purified water to add
Heat is stirred evenly to 65 DEG C, and heat preservation 15min obtains A phase；
4) feedstock oil is weighed by formula ratio and add Tween-80, be heated to 75 DEG C, stir evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, homogeneous 5 minutes, continue to stir 20min, slow cooling to 40
DEG C to get solution.
0.84 part of 2 thick wood-fern rhizome extracting solution of embodiment, 1.65 parts of eucalyptus oil, 1.02 parts of mother chrysanthemum caul-fat, 0.1 part of peppermint oil
1) raw material is weighed, the water that 9 times of medicinal material amounts are added extracts 2 times, each 1h；It filters (200 mesh), merging filtrate, decompression
Being concentrated into relative density is 1.05~1.10；Centrifuged supernatant A is obtained after centrifugation, for use；
2) supernatant A is slowly added to ethyl alcohol, and determining alcohol is made to reach 70%, carries out alcohol precipitation；Centrifugation obtains supernatant B, recycles
Ethyl alcohol is concentrated under reduced pressure into relative density 1.05~1.10, obtains raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and filtering, filtrate adds xanthan gum, glycerol, purified water to add
Heat is stirred evenly to 75 DEG C, and heat preservation 10min obtains A phase；
4) weighing feedstock oil by formula ratio adds Tween-80 to be heated to 75 DEG C, stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, homogeneous 5 minutes, continue to stir 20min, slow cooling to 40
DEG C to get solution.
1.95 parts of 3 Phytolacca acinosa extracting solution of embodiment, 0.25 part of eucalyptus oil, 0.15 part of mother chrysanthemum caul-fat, 0.02 part of cinnamon oil
1) raw material is weighed, the water that 11 times of medicinal material amounts are added extracts 2 times, each 1.5h；It filters (200 mesh), merging filtrate subtracts
It is 1.05~1.10 that pressure, which is concentrated into relative density,；Centrifuged supernatant A is obtained after centrifugation, for use；
2) supernatant A is slowly added to ethyl alcohol, and determining alcohol is made to reach 70%, carries out alcohol precipitation；Centrifugation obtains supernatant B, recycles
Ethyl alcohol is concentrated under reduced pressure into relative density 1.05~1.10, obtains raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and filtering, filtrate adds xanthan gum, glycerol, purified water to add
Heat is stirred evenly to 75 DEG C, and heat preservation 10min obtains A phase；
4) weighing feedstock oil by formula ratio adds Tween-80 to be heated to 75 DEG C, stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, homogeneous 5 minutes, continue to stir 20min, slow cooling to 40
DEG C to get solution.
0.01 part of 4 thick wood-fern rhizome extracting solution of embodiment, 50 parts of eucalyptus oil, 0.01 part of mother chrysanthemum caul-fat, 0.001 part of peppermint oil
1) raw material is weighed, the water that 10 times of medicinal material amounts are added extracts 2 times, each 1h；It filters (200 mesh), merging filtrate, decompression
Being concentrated into relative density is 1.05~1.10；Centrifuged supernatant A is obtained after centrifugation, for use；
2) supernatant A is slowly added to ethyl alcohol, and determining alcohol is made to reach 70%, carries out alcohol precipitation；Centrifugation obtains supernatant B, recycles
Ethyl alcohol is concentrated under reduced pressure into relative density 1.05~1.10, obtains raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and filtering, filtrate adds xanthan gum, glycerol, purified water to add
Heat is stirred evenly to 80 DEG C, and heat preservation 10min obtains A phase；
4) weighing feedstock oil by formula ratio adds Tween-80 to be heated to 65 DEG C, stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, homogeneous 5 minutes, continue to stir 20min, slow cooling to 40
DEG C to get solution.
50 parts of 5 Phytolacca acinosa extracting solution of embodiment, 20 parts of eucalyptus oil, 0.001 part of mother chrysanthemum caul-fat, 10 parts of cinnamon oil
1) A group is prepared: each raw material is weighed by formula rate, after glycerol and butanediol wetting is added in carbomer, xanthan gum,
Add purified water, is swollen 6h, is heated to 70 DEG C；
2) B group is prepared: purified water is added in methyl hydroxybenzoate, is stirred and heated to 70 DEG C, is completely dissolved to material, adds
Bright matter acid sodium, purified water, stir evenly, constant temperature 15min, spare；
3) C group is prepared: raw extract adds purified water to dissolve, filtering, spare；
4) D group is prepared: feedstock oil, Tween-80 are mixed evenly, and are heated to 75 DEG C；
5) D group is added in B group, stirred evenly；It is stirring while adding by 3 addition B, D mixing groups of component A, it is heated to 60
DEG C, it stirs evenly, is cooled to 45 DEG C；C group is added to stir evenly, well pre-mixed Phenoxyethanol, butanediol stirring is then added
Uniformly；It is eventually adding the triethanolamine mixed, purified water stirs evenly to get gelling agent.
1 part of 6 cortex cinnamomi extracting solution of embodiment, 20 parts of cinnamon oil, 30 parts of eucalyptus oil, 10 parts of peppermint oil
1) A group is prepared: each raw material is weighed by formula rate, after glycerol and butanediol wetting is added in carbomer, xanthan gum,
Add purified water, is swollen 6h, is heated to 72 DEG C；
2) B group is prepared: purified water is added in methyl hydroxybenzoate, is stirred and heated to 72 DEG C, is completely dissolved to material, adds
Bright matter acid sodium, purified water, stir evenly, constant temperature 15min, spare；
3) C group is prepared: raw material extracting solution adds purified water to dissolve, filtering, spare；
4) D group is prepared: feedstock oil, Tween-80 are mixed evenly, and are heated to 68 DEG C；
5) D group is added in B group, stirred evenly；It is stirring while adding by 3 addition B, D mixing groups of component A, it is heated to 60
DEG C, it stirs evenly, is cooled to 45 DEG C；C group is added to stir evenly, well pre-mixed Phenoxyethanol, butanediol stirring is then added
Uniformly；It is eventually adding the triethanolamine mixed, purified water stirs evenly to get gelling agent.
0.1 part of 7 cortex cinnamomi extracting solution of embodiment, 0.001 part of cinnamon oil, 50 parts of eucalyptus oil, 20 parts of peppermint oil
1) cortex cinnamomi is weighed by formula ratio, the water that 9 times of medicinal material amounts are added extracts 2 times, and each 1h is concentrated under reduced pressure into relative density
It is 1.05~1.10, concentrate is centrifuged to obtain supernatant A, for use；
2) supernatant A is made into containing 70% solution of amount of alcohol, and alcohol precipitation is carried out after stirring, and centrifugation obtains supernatant B, recycles second
Alcohol, being concentrated under reduced pressure into relative density is 1.05~1.10, obtains cortex cinnamomi extracting solution, spare；
3) sodium acrylate/sodium acryloyldimethyl taurate copolymers are weighed, after glycerol, propylene glycol complete wetting is added,
Again plus purified water stir evenly swelling completely it is spare；Raw material extracting solution is taken, remaining purified water is added, stirs evenly, filters, sorbic acid is added
Potassium stirs evenly, and adds above-mentioned mixed solution, mixes, 85 DEG C keep the temperature to obtain A phase, spare；
4) it weighs feedstock oil to be uniformly mixed, is heated to 80 DEG C, be B phase, it is spare；
5) under stiring, A phase is slowly added in B phase, stirring while adding, homogeneous 5min, continues to stir 20min, slowly
Room temperature is down to get ointment.
50 parts of 8 thick wood-fern rhizome extracting solution of embodiment, 0.01 part of eucalyptus oil, 50 parts of mother chrysanthemum caul-fat, 20 parts of peppermint oil
1) raw material is weighed, the water that 11 times of medicinal material amounts are added extracts 2 times, each 2h；It filters (200 mesh), merging filtrate, decompression
Being concentrated into relative density is 1.05~1.10；Centrifuged supernatant A is obtained after centrifugation, for use；
2) supernatant A is slowly added to ethyl alcohol, and determining alcohol is made to reach 70%, carries out alcohol precipitation；Centrifugation obtains supernatant B, recycles
Ethyl alcohol is concentrated under reduced pressure into relative density 1.05~1.10, obtains raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and filtering, filtrate adds carbomer, propylene glycol, purified water
75 DEG C are heated to, is stirred evenly, heat preservation 15min obtains A phase；
4) weighing feedstock oil by formula ratio adds Tween-80 to be heated to 65 DEG C, stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, homogeneous 5 minutes, continue to stir 20min, slow cooling to 40
DEG C to get solution.
50 parts of 9 thick wood-fern rhizome extracting solution of embodiment, 0.01 part of eucalyptus oil, 50 parts of mother chrysanthemum caul-fat, 20 parts of peppermint oil
1) raw material is weighed, the water that 10 times of medicinal material amounts are added extracts 2 times, each 1.5h；It filters (200 mesh), merging filtrate subtracts
It is 1.05~1.10 that pressure, which is concentrated into relative density,；Centrifuged supernatant A is obtained after centrifugation, for use；
2) supernatant A is slowly added to ethyl alcohol, and determining alcohol is made to reach 70%, carries out alcohol precipitation；Centrifugation obtains supernatant B, recycles
Ethyl alcohol is concentrated under reduced pressure into relative density 1.05~1.10, obtains raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and filtering, filtrate adds xanthan gum, glycerol, purified water to add
Heat is stirred evenly to 75 DEG C, and heat preservation 15min obtains A phase；
4) weighing feedstock oil by formula ratio adds Tween-80 to be heated to 75 DEG C, stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, homogeneous 5 minutes, continue to stir 20min, slow cooling to 40
DEG C to get solution.
1.95 parts of 10 Phytolacca acinosa extracting solution of embodiment, 0.25 part of eucalyptus oil, 0.15 part of mother chrysanthemum caul-fat, 0.02 part of cinnamon oil
1) raw material is weighed, the water that 9 times of medicinal material amounts are added extracts 2 times, each 2h；It filters (200 mesh), merging filtrate, decompression
Being concentrated into relative density is 1.05~1.10；Centrifuged supernatant A is obtained after centrifugation, for use；
2) supernatant A is slowly added to ethyl alcohol, and determining alcohol is made to reach 70%, carries out alcohol precipitation；Centrifugation obtains supernatant B, recycles
Ethyl alcohol is concentrated under reduced pressure into relative density 1.05~1.10, obtains raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and filtering, filtrate adds carbomer, propylene glycol, purified water
65 DEG C are heated to, is stirred evenly, heat preservation 15min obtains A phase；
4) weighing feedstock oil by formula ratio adds Tween-80 to be heated to 75 DEG C, stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, homogeneous 5 minutes, continue to stir 20min, slow cooling to 40
DEG C to get solution.
1.95 parts of 11 Phytolacca acinosa extracting solution of embodiment, 0.25 part of eucalyptus oil, 0.15 part of mother chrysanthemum caul-fat, 0.02 part of cinnamon oil
1) raw material is weighed, the water that 10 times of medicinal material amounts are added extracts 2 times, each 1.5h；It filters (200 mesh), merging filtrate subtracts
It is 1.05~1.10 that pressure, which is concentrated into relative density,；Centrifuged supernatant A is obtained after centrifugation, for use；
2) supernatant A is slowly added to ethyl alcohol, and determining alcohol is made to reach 70%, carries out alcohol precipitation；Centrifugation obtains supernatant B, recycles
Ethyl alcohol is concentrated under reduced pressure into relative density 1.05~1.10, obtains raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and filtering, filtrate adds hypromellose, the third two
Alcohol, purified water are heated to 75 DEG C, stir evenly, and heat preservation 15min obtains A phase；
4) weighing feedstock oil by formula ratio adds Tween-80 to be heated to 75 DEG C, stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, homogeneous 5 minutes, continue to stir 20min, slow cooling to 40
DEG C to get solution.
100 parts of 12 cortex cinnamomi extracting solution of embodiment, 50 parts of cinnamon oil, 0.001 part of eucalyptus oil, 0.001 part of peppermint oil
1) A group is prepared: each raw material is weighed by formula rate, after glycerol and butanediol wetting is added in carbomer, xanthan gum,
Add purified water, is swollen 6h, is heated to 70 DEG C；
2) B group is prepared: purified water is added in methyl hydroxybenzoate, is stirred and heated to 75 DEG C, is completely dissolved to material, adds
Bright matter acid sodium, purified water, stir evenly, constant temperature 15min, spare；
3) C group is prepared: raw material extracting solution adds purified water to dissolve, filtering, spare；
4) D group is prepared: feedstock oil, Tween-80 are mixed evenly, and are heated to 70 DEG C；
5) D group is added in B group, stirred evenly；It is stirring while adding by 3 addition B, D mixing groups of component A, it is heated to 60
DEG C, it stirs evenly, is cooled to 45 DEG C；C group is added to stir evenly, well pre-mixed Phenoxyethanol, butanediol stirring is then added
Uniformly；It is eventually adding the triethanolamine mixed, purified water stirs evenly to get gelling agent.
50 parts of 13 cortex cinnamomi extracting solution of embodiment, 0.01 part of cinnamon oil, 0.01 part of eucalyptus oil, 0.005 part of peppermint oil
1) A group is prepared: each raw material is weighed by formula rate, after glycerol and butanediol wetting is added in carbomer, xanthan gum,
Add purified water, is swollen 6h, is heated to 70 DEG C；
2) B group is prepared: purified water is added in methyl hydroxybenzoate, is stirred and heated to 70 DEG C, is completely dissolved to material, adds
Bright matter acid sodium, purified water, stir evenly, constant temperature 15min, spare；
3) C group is prepared: raw material extracting solution adds purified water to dissolve, filtering, spare；
4) D group is prepared: feedstock oil, Tween-80 are mixed evenly, and are heated to 70 DEG C；
5) D group is added in B group, stirred evenly；It is stirring while adding by 3 addition B, D mixing groups of component A, it is heated to 60
DEG C, it stirs evenly, is cooled to 40 DEG C；C group is added to stir evenly, well pre-mixed Phenoxyethanol, butanediol stirring is then added
Uniformly；It is eventually adding the triethanolamine mixed, purified water stirs evenly to get gelling agent.
0.2 part of 14 Phytolacca acinosa extracting solution of embodiment, 0.1 part of eucalyptus oil, 10 parts of mother chrysanthemum caul-fat, 5 parts of cinnamon oil
1) A group is prepared: each raw material is weighed by formula rate, after glycerol and butanediol wetting is added in carbomer, xanthan gum,
Add purified water, is swollen 6h, is heated to 70 DEG C；
2) B group is prepared: purified water is added in methyl hydroxybenzoate, is stirred and heated to 75 DEG C, is completely dissolved to material, adds
Bright matter acid sodium, purified water, stir evenly, constant temperature 15min, spare；
3) C group is prepared: raw material extracting solution adds purified water to dissolve, filtering, spare；
4) D group is prepared: feedstock oil, Tween-80 are mixed evenly, and are heated to 70 DEG C；
5) D group is added in B group, stirred evenly；It is stirring while adding by 3 addition B, D mixing groups of component A, it is heated to 60
DEG C, it stirs evenly, is cooled to 45 DEG C；C group is added to stir evenly, well pre-mixed Phenoxyethanol, butanediol stirring is then added
Uniformly；It is eventually adding the triethanolamine mixed, purified water stirs evenly to get gelling agent.
20 parts of 15 cortex cinnamomi extracting solution of embodiment, 0.15 part of cinnamon oil, 1 part of eucalyptus oil, 2 parts of peppermint oil
1) raw material is weighed, the water that 10 times of medicinal material amounts are added extracts 2 times, each 1h；It filters (200 mesh), merging filtrate, decompression
Being concentrated into relative density is 1.05~1.10；Centrifuged supernatant A is obtained after centrifugation, for use；
2) supernatant A is slowly added to ethyl alcohol, and determining alcohol is made to reach 70%, carries out alcohol precipitation；Centrifugation obtains supernatant B, recycles
Ethyl alcohol is concentrated under reduced pressure into relative density 1.05~1.10, obtains raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and filtering, filtrate adds xanthan gum, butanediol, purified water
65 DEG C are heated to, is stirred evenly, heat preservation 15min obtains A phase；
4) weighing feedstock oil by formula ratio adds Tween-80 to be heated to 75 DEG C, stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, homogeneous 5 minutes, continue to stir 20min, slow cooling to 40
DEG C to get solution.
0.8 part of 16 thick wood-fern rhizome extracting solution of embodiment, 5.2 parts of eucalyptus oil, 8.9 parts of mother chrysanthemum caul-fat, 6 parts of peppermint oil
1) raw material is weighed by formula ratio, the water that 9 times of medicinal material amounts are added extracts 2 times, and each 1h is concentrated under reduced pressure into relative density
It is 1.05~1.10, concentrate is centrifuged to obtain supernatant A, for use；
2) supernatant A is made into containing 70% solution of amount of alcohol, and alcohol precipitation is carried out after stirring, and centrifugation obtains supernatant B, recycles second
Alcohol, being concentrated under reduced pressure into relative density is 1.05~1.10, obtains raw material extracting solution, spare；
3) sodium acrylate/sodium acryloyldimethyl taurate copolymers are weighed, after glycerol, propylene glycol complete wetting is added,
Again plus purified water stir evenly swelling completely it is spare；Raw material extracting solution is taken, remaining purified water is added, stirs evenly, filters, sorbic acid is added
Potassium stirs evenly, and adds above-mentioned mixed solution, mixes, 80 DEG C keep the temperature to obtain A phase, spare；
4) it weighs feedstock oil to be uniformly mixed, is heated to 80 DEG C, be B phase, it is spare；
5) under stiring, A phase is slowly added in B phase, stirring while adding, homogeneous 5min, continues to stir 20min, slowly
Room temperature is down to get ointment.
10 parts of 17 Phytolacca acinosa extracting solution of embodiment, 3 parts of eucalyptus oil, 5 parts of mother chrysanthemum caul-fat, 0.1 part of cinnamon oil
1) raw material is weighed by formula ratio, the water that 10 times of medicinal material amounts are added extracts 2 times, each 1h, is concentrated under reduced pressure into relatively close
Degree is 1.05~1.10, and concentrate is centrifuged to obtain supernatant A, for use；
2) supernatant A is made into containing 70% solution of amount of alcohol, and alcohol precipitation is carried out after stirring, and centrifugation obtains supernatant B, recycles second
Alcohol, being concentrated under reduced pressure into relative density is 1.05~1.10, obtains raw material extracting solution, spare；
3) sodium acrylate/sodium acryloyldimethyl taurate copolymers are weighed, after glycerol, propylene glycol complete wetting is added,
Again plus purified water stir evenly swelling completely it is spare；Raw material extracting solution is taken, remaining purified water is added, stirs evenly, filters, sorbic acid is added
Potassium stirs evenly, and adds above-mentioned mixed solution, mixes, 87 DEG C keep the temperature to obtain A phase, spare；
4) it weighs feedstock oil to be uniformly mixed, is heated to 80 DEG C, be B phase, it is spare；
5) under stiring, A phase is slowly added in B phase, stirring while adding, homogeneous 5min, continues to stir 20min, slowly
Room temperature is down to get ointment.
0.24 part of 18 cinnamon oil of embodiment, 0.1 part of eucalyptus oil, 0.01 part of peppermint oil
Cinnamon oil, eucalyptus oil, peppermint oil mixing are weighed by formula ratio to get finish.
1.65 parts of 19 eucalyptus oil of embodiment, 1.02 parts of mother chrysanthemum caul-fat, 0.1 part of peppermint oil
Eucalyptus oil, mother chrysanthemum caul-fat, peppermint oil mixing are weighed by formula ratio to get finish.
0.25 part of 20 eucalyptus oil of embodiment, 0.15 part of mother chrysanthemum caul-fat, 0.02 part of cinnamon oil
Eucalyptus oil, mother chrysanthemum caul-fat, cinnamon oil mixing are weighed by formula ratio to get finish.
0.01 part of 21 Phytolacca acinosa extracting solution of embodiment, 0.001 part of eucalyptus oil, 20 parts of mother chrysanthemum caul-fat, 0.001 part of cinnamon oil
1) raw material is weighed, the water that 10 times of medicinal material amounts are added extracts 2 times, each 1.5h；It filters (200 mesh), merging filtrate subtracts
It is 1.05~1.10 that pressure, which is concentrated into relative density,；Centrifuged supernatant A is obtained after centrifugation, for use；
2) supernatant A is slowly added to ethyl alcohol, and determining alcohol is made to reach 70%, carries out alcohol precipitation；Centrifugation obtains supernatant B, recycles
Ethyl alcohol is concentrated under reduced pressure into relative density 1.05~1.10, obtains raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and filtering, filtrate adds xanthan gum, butanediol, purified water
75 DEG C are heated to, is stirred evenly, heat preservation 15min obtains A phase；
4) weighing feedstock oil by formula ratio adds Tween-80 to be heated to 75 DEG C, stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, homogeneous 5 minutes, continue to stir 20min, slow cooling to 40
DEG C to get solution.
0.3 part of 22 thick wood-fern rhizome extracting solution of embodiment, 0.3 part of eucalyptus oil, 20 parts of mother chrysanthemum caul-fat, 10 parts of peppermint oil
1) raw material is weighed, the water that 10 times of medicinal material amounts are added extracts 2 times, each 1h；It filters (200 mesh), merging filtrate, decompression
Being concentrated into relative density is 1.05~1.10；Centrifuged supernatant A is obtained after centrifugation, for use；
2) supernatant A is slowly added to ethyl alcohol, and determining alcohol is made to reach 70%, carries out alcohol precipitation；Centrifugation obtains supernatant B, recycles
Ethyl alcohol is concentrated under reduced pressure into relative density 1.05~1.10, obtains raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and filtering, filtrate adds xanthan gum, glycerol, purified water to add
Heat is stirred evenly to 70 DEG C, and heat preservation 10min obtains A phase；
4) weighing feedstock oil by formula ratio adds Tween-80 to be heated to 75 DEG C, stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, homogeneous 5 minutes, continue to stir 20min, slow cooling to 40
DEG C to get solution.
25 parts of 23 cortex cinnamomi extracting solution of embodiment, 3 parts of cinnamon oil, 0.008 part of eucalyptus oil, 0.004 part of peppermint oil
1) raw material is weighed, the water that 9 times of medicinal material amounts are added extracts 2 times, each 1h；It filters (200 mesh), merging filtrate, decompression
Being concentrated into relative density is 1.05~1.10；Centrifuged supernatant A is obtained after centrifugation, for use；
2) supernatant A is slowly added to ethyl alcohol, and determining alcohol is made to reach 70%, carries out alcohol precipitation；Centrifugation obtains supernatant B, recycles
Ethyl alcohol is concentrated under reduced pressure into relative density 1.05~1.10, obtains raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and filtering, filtrate adds xanthan gum, glycerol, purified water to add
Heat is stirred evenly to 65 DEG C, and heat preservation 15min obtains A phase；
4) weighing feedstock oil by formula ratio adds Tween-80 to be heated to 75 DEG C, stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, homogeneous 5 minutes, continue to stir 20min, slow cooling to 40
DEG C to get solution.
0.1 part of 24 thick wood-fern rhizome extracting solution of embodiment, 0.17 part of eucalyptus oil, 0.23 part of mother chrysanthemum caul-fat, 12.5 parts of peppermint oil
1) raw material is weighed, the water that 9 times of medicinal material amounts are added extracts 2 times, each 1h；It filters (200 mesh), merging filtrate, decompression
Being concentrated into relative density is 1.05~1.10；Centrifuged supernatant A is obtained after centrifugation, for use；
2) supernatant A is slowly added to ethyl alcohol, and determining alcohol is made to reach 70%, carries out alcohol precipitation；Centrifugation obtains supernatant B, recycles
Ethyl alcohol is concentrated under reduced pressure into relative density 1.05~1.10, obtains raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and filtering, filtrate adds xanthan gum, glycerol, purified water to add
Heat is stirred evenly to 75 DEG C, and heat preservation 15min obtains A phase；
4) weighing feedstock oil by formula ratio adds Tween-80 to be heated to 75 DEG C, stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, homogeneous 5 minutes, continue to stir 20min, slow cooling to 40
DEG C to get solution.
12 parts of 25 Phytolacca acinosa extracting solution of embodiment, 0.07 part of eucalyptus oil, 0.05 part of mother chrysanthemum caul-fat, 0.008 part of cinnamon oil
1) raw material is weighed, the water that 11 times of medicinal material amounts are added extracts 2 times, each 1.5h；It filters (200 mesh), merging filtrate subtracts
It is 1.05~1.10 that pressure, which is concentrated into relative density,；Centrifuged supernatant A is obtained after centrifugation, for use；
2) supernatant A is slowly added to ethyl alcohol, and determining alcohol is made to reach 70%, carries out alcohol precipitation；Centrifugation obtains supernatant B, recycles
Ethyl alcohol is concentrated under reduced pressure into relative density 1.05~1.10, obtains raw material extracting solution, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, and filtering, filtrate adds xanthan gum, Tween-80, purified water
75 DEG C are heated to, is stirred evenly, heat preservation 15min obtains A phase；
4) weighing feedstock oil by formula ratio adds Tween-80 to be heated to 75 DEG C, stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, homogeneous 5 minutes, continue to stir 20min, slow cooling to 40
DEG C to get solution.
Pass through the beneficial effect of following experiment the present invention is further explained the composition
One, present composition anti-bacteria and anti-virus performance detection
1. experimental material
Given the test agent: embodiment 1,2,3,23,24,25 prepares resulting composition, respectively experimental group 1,2,3,4,5,6
Compare given the test agent: embodiment 18,19,20 prepares resulting composition, respectively control experiment group 1,2,3
Bacterium: staphylococcus aureus and Escherichia coli
Cell: dog renal epithelial cell (Madin-Darby Canine Kidney Cells, MDCK)
Virus: H7N3 (A/China/24/96) and H9N2 (A/HK/Y280/97) avian influenza virus.
2. method
2.1 present composition anti-microbial property detection methods
1) taking diameter is 5mm, and sterile and dry filter paper, every 20 μ l of given the test agent, blank control group is added dropwise sterile
20 μ l of distilled water, then lies against filter paper in clean sterilized petri dishes, sets stand-by after spontaneously drying at room temperature.
2) dipping concentration with sterile cotton swab is that 5 × 105cfu/ml tests bacteria suspension, in nutrient agar plate table
It uniformly smears 3 times in face.Every to smear 1 time, plate should rotate 60 °, finally smear cotton swab one week around plate edge.It covers flat
Ware sets drying at room temperature 5min.
3) it is equidistantly placed with aseptic nipper coupongs in planar surface.After being placed with, with the light pressure-like piece of aseptic nipper, make it
It is tightly attached to planar surface.Plate is covered, 37 DEG C of incubators are set, culture 18h observes result.With the diameter of vernier caliper measurement antibacterial ring size
(including patch) simultaneously records.
The judgement of bacteriostasis: inhibition zone diameter is greater than 7mm person, has been judged to bacteriostasis；Inhibition zone diameter is less than or waits
In 7mm person, it is judged to no bacteriostasis.
The detection of 2.2 present composition antiviral properties
2.2.1 96 orifice plate cell monolayers are abandoned supernatant, 9 embodiment sample solution, 100 μ L/ are added with PBS washing 1 time
Hole.The culture medium of respective volume, 37 DEG C, 5%CO are added in blank control group and the every hole of normal cell group2Under the conditions of cultivate 2 hours,
Culture medium culture 2 days for being free of serum are added in the supernatant removing medicine group and blank group after 2 hours and normally organizing.Then add
Enter 20 hole μ L/ of MTT solution (5mg/mL), continues to be incubated for 4 hours.Supernatant is abandoned, adds 100 μ L/ hole DMSO, low-speed oscillation 10 divides
Clock melts crystal sufficiently.490nm wavelength is selected, measures light absorption value on enzyme linked immunological instrument.Calculate inhibiting rate.
The drug concentration of 50% inhibiting rate, as drug half toxic concentration (TC50) are calculated with Reed-Muench method.
Note: inhibiting rate=[(normally organizing mean OD value-blank group mean OD value)-(administration group mean OD value-blank group
Mean OD value)]/(normally organizing mean OD value-blank group mean OD value) × 100%
2.2.2 initial concentration is diluted to greater than maximal non-toxic concentration (TC0) according to drug toxicity with 2ml centrifuge tube.?
Initial concentration is carried out gradient dilution.
Dilute intact, 37 DEG C of virus liquid of the drug effect group of each gradient dilution and 200TCID50 titre are incubated for 1 hour；
After 1 hour, the drug effect group of drug and virus incubation and virus-free incubation medicine group are added to containing MDCK cell monolayers
96 orifice plates in, the supernatant of drug effect group and medicine group is removed after being incubated for 2 hours, 2 hours, is added the 1.5ug/mL's containing final concentration
The hole virus-culturing fluid 100uL/, after 48 hours, MTT solution (5mg/mL) 20 μ L is added in every hole, sets 37 DEG C, 5%CO2 incubator relaying
It is continuous to be incubated for 4 hours.It inhales and abandons supernatant, then 100 μ L 2- methyl sulfoxides (DMSO) are added in every hole, low-speed oscillation 10 minutes, make to crystallize
Object sufficiently melts.490nm wavelength is selected, each hole absorbance value is measured on enzyme linked immunological monitor.
Drug medium effective concentration (IC50) is calculated with Reed-Muench method, calculates selection index
(SelectiveIndex, SI): SI=TC50/IC50).Referring to " pharmacological experimental methodology, Xu Shuyun chief editor ", SI > 1 is indicated
Effect.
3. experimental result
3.1 present composition anti-microbial property testing results
1 present composition anti-microbial property testing result of table
As seen from Table 1, in addition to blank control group, experimental group 1-6 has significantly staphylococcus aureus and Escherichia coli
Inhibiting effect；Control experiment group also has an inhibiting effect to staphylococcus aureus and Escherichia coli, but effect is not as good as experimental group,
Show that the present composition has antibacterial functions, and there is synergistic function between each ingredient.
3.2 present compositions are in vitro to the inhibiting effect of fowl Influenza Virus H7N3 and H9N2 Direct Model
2 present composition of table is in vitro to the inhibiting effect of fowl Influenza Virus H7N3 and H9N2 Direct Model
As seen from Table 1, in addition to blank control group, experimental group 1-6 is to H7N3 (A/China/24/96) and H9N2 (A/HK/
Y280/97) avian influenza virus has killing effect；Control experiment group is to H7N3 (A/China/24/96) and H9N2 (A/HK/
Y280/97) avian influenza virus also has killing effect, but it is anti-to show that the present composition has not as good as other experimental groups for effect
Viral function, and there is synergistic function between each ingredient.
Remaining embodiment of the invention has carried out similar test according to the method described above, as a result identical as the above results.
Two, the present composition is to disinfection of indoor air effect observation
1. experimental method
1.1 test material
Embodiment 18,19,20 prepare composition (being experimental group 1,2,3 respectively), aroma burner (laboratory offers), commonly
Nutrient agar plate, mobile two-tube ultraviolet light, incubator.
1.2 sterilizing test steps
1.2.1 sampling before disinfection
Between choice experiment room one, chamber interior volume about 35m3, it tests and is carried out under unmanned static state, room temperature 23 DEG C~25
DEG C, relative humidity 40%~60%.Before test, with the ordinary nutrient agar culture medium flat plate of diameter 9cm, use as required at 5 points
Plate is laid, flat plate cover natural subsidence 10min sampling is opened, as control before disinfection.
1.2.2 sterilizing test
When experiment, experimental group 1,2,3 is placed in aroma burner fumigates respectively, closed the doors and windows, fumigates 1h.Control group uses
Mobile two-tube ultraviolet light, is placed in room center, fluorescent tube 1.5m, irradiation time 1h from the ground.Respectively in 2h, 4h, for 24 hours when, press
The above-mentioned method of sampling carries out disinfection post-sampling.
All sampling plates are placed in 37 DEG C of incubators and cultivate 48h, count flat-plate bacterial colony number, calculate total number of bacteria and bacterium
Reduced rate.
It carries out weekly testing for 1 time, repeated observation 5 times.
2. result
Total Bacteria Count of Bacteria in Indoor Air compares after 3 two kinds of disinfection methods of table
Air sterilization effect compares in 4 two kinds of disinfection methods rear chambers of table
From table 3,4 as it can be seen that the present composition is better than ultraviolet irradiation, and bacterium in air to the killing effect of bacterium
Total rise speed ratio is slow in ultraviolet sterilization total number of bacteria rise rate.Show that the present composition has disinfection function
Energy.
Three, safety experiment
1. experimental material
Experimental animal: healthy rabbits
Laboratory sample: composition obtained by embodiment 1-25
2. experimental method
Animal skin stimulation test: taking healthy rabbits 125, is randomly divided into 25 groups, every group 5, backbone diamond wool is cut
Fall (about 3cm*3cm), epidermis can not be damaged, embodiment each group composition is coated onto respectively on the skin of side, with two layers of gauze and
Glassine paper covering, then fixed with nonirritant adhesive plaster and bandage；Tested material is removed after 4 hours as control in the other side, in
Position is smeared in observation after 1 hour, 24 hours, 48 hours, 72 hours, is showed no erythema, oedema phenomenon.
3. experimental result
Rabbit smears position and is showed no erythema, oedema phenomenon, table through observation after 1 hour, 24 hours, 48 hours, 72 hours
Bright, the nonirritant effect of the present composition can be used safely.
Claims (12)
1. a kind of composition with anti-bacteria and anti-virus, which is characterized in that the composition is extracted by Ginger P.E, myrobalan
Object, Herba Agrimoniae extract, nutgall extractive, Cacumen Securinegae Suffruticosae extract, Rhizoma Dryopteris Crassirhizomatis extract, pokeberry extract, cloves are extracted
Object, cinnamomum cassia extract, Flos Chrysanthemi Indici extract, folium eucalypti extract, forsythia suspense extraction, Radix Stemonae extract, Geranium extract, rhizoma atractylodis
Extract, Folium Artemisiae Argyi extract, Herba Lysimachiae foenumgraeci extract, Radix agastaches extract, Turmeric P.E, choose any one kind of them in mint extract or
More than one compositions.
2. composition according to claim 1, which is characterized in that the composition is extracted by ginger extract, hairyvein agrimony
Liquid, Common Leafflower Herb extracting solution, thick wood-fern rhizome extracting solution, Phytolacca acinosa extracting solution, cortex cinnamomi extracting solution, wild chrysanthemum extracting solution, folium eucalypti extracting solution,
Tuber of stemona extracting solution, geranium wilfordii extracting solution, Atractylis oil, Blumea oil, cinnamon oil, lavender oil, mother chrysanthemum caul-fat, patchouli oil, folium eucalypti
Oil, curcuma oil are chosen any one kind of them in peppermint oil or more than one compositions.
3. composition according to claim 2, which is characterized in that the composition is extracted by ginger extract, hairyvein agrimony
Liquid, thick wood-fern rhizome extracting solution, cortex cinnamomi extracting solution, wild chrysanthemum extracting solution, folium eucalypti extracting solution, tuber of stemona extracting solution, geranium wilfordii extracting solution,
Blumea oil, lavender oil, eucalyptus oil, curcuma oil, is chosen any one kind of them in peppermint oil or more than one compositions at cinnamon oil.
4. composition according to claim 3, which is characterized in that the composition by thick wood-fern rhizome extracting solution, eucalyptus oil,
Mother chrysanthemum caul-fat, peppermint oil composition are made of cortex cinnamomi extracting solution, cinnamon oil, eucalyptus oil, peppermint oil or by Phytolacca acinosa extracting solution, folium eucalypti
Oil, mother chrysanthemum caul-fat, cinnamon oil composition.
5. composition according to claim 4, which is characterized in that the composition is formed by following weight: continuous
0.01-50 parts of horse rhizome of cyrtomium extracting solution, 0.01-50 parts of eucalyptus oil, 0.01-50 parts of mother chrysanthemum caul-fat, 0.001-20 parts of peppermint oil or
0.1-100 parts of cortex cinnamomi extracting solution, 0.0041-50 parts of cinnamon oil, 0.001-50 parts of eucalyptus oil, 0.001-20 parts of peppermint oil or
0.01-50 parts of Phytolacca acinosa extracting solution, 0.001-20 parts of eucalyptus oil, 0.001-20 parts of mother chrysanthemum caul-fat, 0.001-10 parts of cinnamon oil.
6. composition according to claim 6, which is characterized in that the composition is formed by following weight: continuous
0.3-20 parts of horse rhizome of cyrtomium extracting solution, 0.3-20 parts of eucalyptus oil, 0.3-20 parts of mother chrysanthemum caul-fat, 0.1-10 parts of peppermint oil or
1-50 parts of cortex cinnamomi extracting solution, 0.01-20 parts of cinnamon oil, 0.01-30 parts of eucalyptus oil, 0.005-10 parts of peppermint oil or
0.2-20 parts of Phytolacca acinosa extracting solution, 0.1-10 parts of eucalyptus oil, 0.1-10 parts of mother chrysanthemum caul-fat, 0.01-5 parts of cinnamon oil.
7. a kind of method for preparing any one of claim 1-6 composition, which is characterized in that the composition is by with lower section
Method is prepared:
1) weighing raw material by formula ratio adds the solvent of various concentration individually to extract or mix extraction, and extracting solution is concentrated to give crude extract；
Or it is further steamed using water extraction and alcohol precipitation method, organic solvent extractionprocess, column chromatography, carbon dioxide supercritical extraction method, vapor
The one or more for evaporating method, which are used in combination after progress suitably refines, obtains extract；Above-mentioned crude extract or extract are that raw material extracts
Object；
2) weigh raw extract by formula ratio, add or be not added auxiliary material to get.
8. composition according to claim 1-6, which is characterized in that the composition is exterior-applied formulation, is presented
It is any for solution, pulvis, lotion, finish, tincture, gelling agent, emulsion, ointment, paste, plaster, liniment, plastics, aerosol
A kind of form.
9. composition according to claim 8, which is characterized in that the composition is solution.
10. a kind of method for preparing composition as claimed in claim 9, which is characterized in that the described method comprises the following steps:
1) raw material is weighed, water mentions, and concentration is centrifuged to obtain supernatant A, for use；
2) supernatant A is slowly added to ethyl alcohol, reaches corresponding determining alcohol, carries out alcohol precipitation, is centrifuged to obtain supernatant B, recycles ethyl alcohol, concentration
Raw material extracting solution is obtained, for use；
3) weighing raw material extracting solution by formula ratio adds suitable quantity of water to dissolve, filtering, and filtrate is added moisturizer, thickener, purified water and adds
Heat, heat preservation, obtains A phase；
4) weighing feedstock oil by formula ratio adds emulsifier to heat, and stirs evenly to obtain B phase；
5) A phase is added slowly in B phase, it is stirring while adding, several minutes of homogeneous, continue to stir, slow cooling to obtain the final product.
11. according to the method described in claim 10, it is characterized in that, the moisturizer is glycerol, propylene glycol, 1,3- fourth two
Alcohol, dipropylene glycol, the pure and mild sorbeth -20 of sorb (sugar), amides moisturizer, glycerin polyether class moisturizer, lactic acid and cream
Sour sodium, pyrrolidone sodium carboxylate, ethoxy urea, antierythrite, Sodium Hyaluronate, carbohydrate isomer, D-pantothenyl aleohol, poly- second two
Alcohol, avenabeta glucosan, trehalose, allantoin, silk propylhomoserin, hydrolytic soya bean protein, hydrolysis rice bran protein, hexylene glycol, xylose
One of alcohol, polypropylene glycol, chitin, glycine betaine or more than one；
The thickener is carbomer, xanthan gum, Arabic gum, bassora gum, locust bean gum, sodium carboxymethylcellulose, hydroxypropyl first
Base cellulose, methylcellulose, hydroxyethyl cellulose, acrylic acid (ester) analog copolymer, glycerol polymethacrylate polymer,
Polyvinyl alcohol, ammonium acryloyl taurate/VP copolymer, hydroxy-ethyl acrylate/sodium acryloyldimethyl taurate copolymerization
One of object, polyisobutene, magnesium sulfate, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer or it is a kind of with
On；
The emulsifier is methyl sesquistearate, PEG-20 methyl glucoside sesquialter hard acid ester, stereth-
2, stereth -21, cetearyl alcohol alcohol ether -2, cetearyl alcohol alcohol ether -21, docosanol ether -25, stearine, spit
Temperature -80, Tween-60, Tween-20, cetearyl glucoside/cetostearyl alcohol, stearine, C20-C22 alkylol/
C20 alkyl glucoside, stearoyl-glutamic acid sodium, Sucrose Polystearic Acid Esters/Parleam, -3/ cetostearyl alcohol of polyglycereol
It is olive oleate, acetylation monoglyceride, wool grease, rilanit special, one or more kinds of in palmityl alcohol.
12. any one of -6 compositions or claim 8 or the group of claim 10 the method preparation according to claim 1
Close the purposes of object, it is characterised in that: the composition is in preparing drug, disinfectant, cosmetics, health treatment or amenities
Purposes.
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| CN111184800A (en) * | 2020-03-01 | 2020-05-22 | 北京博爱堂中医药研究所 | Chinese medicinal preparation for inhibiting virus and fungus and activating immune system |
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| CN111686051A (en) * | 2020-07-27 | 2020-09-22 | 浙江爱尚日用品有限公司 | Plant toothpaste for preventing and treating halitosis |
| CN111789796A (en) * | 2020-04-28 | 2020-10-20 | 广东分子态生物股份有限公司 | Disinfectant with liposome-encapsulated bactericidal component and preparation method thereof |
| CN112998012A (en) * | 2021-03-04 | 2021-06-22 | 杭州新福华无纺布有限公司 | Disinfection wet tissue |
| CN115337246A (en) * | 2022-07-11 | 2022-11-15 | 广州摩亚方舟贸易有限公司 | Natural antibacterial and bacteriostatic plant composition and application thereof |
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| CN111184799A (en) * | 2020-02-25 | 2020-05-22 | 北京博爱堂中医药研究所 | Anti-coronavirus traditional Chinese medicine atomization agent |
| CN111184800A (en) * | 2020-03-01 | 2020-05-22 | 北京博爱堂中医药研究所 | Chinese medicinal preparation for inhibiting virus and fungus and activating immune system |
| CN111450297A (en) * | 2020-04-26 | 2020-07-28 | 青岛明月海藻生物健康科技集团有限公司 | Marine fucoidin herbal atomizing agent and preparation method thereof |
| CN111789796A (en) * | 2020-04-28 | 2020-10-20 | 广东分子态生物股份有限公司 | Disinfectant with liposome-encapsulated bactericidal component and preparation method thereof |
| CN111686051A (en) * | 2020-07-27 | 2020-09-22 | 浙江爱尚日用品有限公司 | Plant toothpaste for preventing and treating halitosis |
| CN112998012A (en) * | 2021-03-04 | 2021-06-22 | 杭州新福华无纺布有限公司 | Disinfection wet tissue |
| CN115337246A (en) * | 2022-07-11 | 2022-11-15 | 广州摩亚方舟贸易有限公司 | Natural antibacterial and bacteriostatic plant composition and application thereof |
| CN116643044A (en) * | 2023-06-15 | 2023-08-25 | 广州贝思奇诊断试剂有限公司 | Kit for detecting HIV-1 and HIV-2 antibodies in urine based on colloidal gold method, and preparation method and application thereof |
| CN116643044B (en) * | 2023-06-15 | 2023-12-12 | 广州贝思奇诊断试剂有限公司 | Kit for detecting HIV-1 and HIV-2 antibodies in urine based on colloidal gold method, and preparation method and application thereof |
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