RU2669929C1 - Preparation for treatment of cows suffering from mastitis and method of its production - Google Patents

Abstract

FIELD: veterinary science.SUBSTANCE: invention relates to veterinary medicine, namely to treatment of cows suffering from mastitis. Drug for treatment of cows with mastitis includes a 10 % extract of a mixture of propolis and herbal extracts, the extract of which is a water-propylene glycol mixture with a ratio of water to propylene glycol 1:1, as well as the following substances with the following component ratio, % by weight: arabinogalactan 20, bischofite 20, dimethylsulfoxide 15. Extractable mixture of propolis and herbal extract contains components, mass. %: propolis 15, sage grass 20, leaves of eucalyptus 20, flowers of calendula 10, flowers of chamomile 10, roots of common licorice 15, ground part of bush cinquefoil 10.EFFECT: invention provides reduction in the timing of treatment of animals, as well as a reduction in the timing of utilization of milk.1 cl, 1 dwg, 9 tbl

Description

The invention relates to the field of veterinary medicine, in particular for the treatment of cows with mastitis.

Known drugs for the treatment of cows with mastitis patients using etiotropic drugs. As etiotropic agents use antibiotics, sulfonamides, nitrofurans, medicinal paints. The most common method for treating cows with mastitis is the intracisternal administration of antimicrobial agents to the affected portion of the udder, and their parenteral administration is also possible. The frequency of administration of the drug and the course of treatment are regulated by the instructions for use.

The recovery of animals is controlled 5-7 days after completion of the course of treatment. In the absence of positive results, a repeated course of treatment with the drug is carried out, to which the microflora isolated from the mastitis udder is sensitive (Slobodyanik V.I. Immunological aspects of the physiology and pathology of the mammary gland of cows. / V.I. Slobodyanik, V.A. Parikov, N .T. Klimov [et al.] - Taganrog: Publishing Center Taganrog, State Pedagogical Institute, 2009. - 276 p.).

When using these drugs, a number of negative effects arise, for example, milk with a residual amount of antibiotic substances is not permissible to deliver to milk processing enterprises; microorganisms circulating on the farm develop resistance to the antibiotic preparations used, which means that their use in the treatment of other diseases will not be effective enough; the secondary toxic effect of the use of antibiotic preparations sometimes exceeds the primary therapeutic; in modern conditions, the use of one antibiotic preparation is often not effective enough and you have to use two or more of their names at once; antibiotic preparations can cause drug allergies in animals and humans.

An obvious alternative to the use of antibacterial drugs in the treatment of mastitis in cows is the use of highly effective environmentally friendly therapeutic agents based on raw materials of plant, mineral and animal origin.

It is known in the treatment of subclinical mastitis in cows the use of decoctions of such plants as eucalyptus, calendula, St. John's wort (Popov L.K. Treatment of mastitis with herbal remedies and stress corrector Ligfall / L.K. Popov, A.N. Gavrin // Zootechny. - 2008, No. 7, S. 26-27).

A known preparation based on components of Siberian fir for the treatment of mastitis in cows, including dimethyl sulfoxide as one of its components (KZ 23945, 05.16.2011). Its disadvantage is that it was created on an oil basis, which prevents its complete release and penetration of antibiotics into the tissues of the udder. In addition, the vegetable oils that make up the preparations are oxidized during prolonged storage.

Known drug containing as one of the components of the natural mineral bischofite (RU 2417089, 04/27/2011).

A known drug that includes propolis extract in the composition of the ingredients (RU 2102074, 01.20.1995).

The disadvantage of this drug, as well as preparations based on plant materials, is that they are most often used to treat subclinical mastitis, and in the treatment of clinically expressed mastitis, they are not effective enough and do not have a multidirectional pharmacological effect.

The closest in its technical essence is the anti-mastitis drug "PFP" contains dioxidine, probiotic Lactimet, propolis extract, herbal extract (bearberry, St. John's wort), nipagin, sodium carboxymethyl cellulose. It is intended for the treatment of cows with subclinical and serous-catarrhal clinical mastitis in the lactation period. (Ivashkevich O.P. Efficiency of dioxidine-based drugs and veterinary and sanitary assessment of livestock products and their use / O.P. Ivashkevich // Uch.Zapiski Order of the Badge of Honor of the State Academy of Veterinary Medicine. - Vitebsk, 2015. - T. 51, issue 1. - Part 1. - S. 45-47).

The disadvantage of this drug is that there is a need for rejection of milk during and after treatment for 72 hours due to the allocation of dioxidine with milk.

The problem solved by the present invention is the creation of a drug and its preparation technology containing several actively active substances from different class compounds, mutually complementing and enhancing the therapeutic effect of each other, as well as expanding the range of environmentally friendly drugs.

The present problem is solved in that the preparation for treating cows with mastitis patients, including propolis extract and herbal extract, additionally contains arabinogalactan, bischofite, dimethyl sulfoxide, and water-propylene glycol mixture in the ratio of water and propylene glycol 1 as the extractant of the propolis and herbal mixture: 1, in the following ratio of components, wt. %:

Arabinogalactan twenty Bischofite twenty Dimethyl sulfoxide fifteen 10% - water-propylene glycol extract mixtures of propolis and herbal rest,

where the mixture of propolis and grass collection contains propolis, sage grass, eucalyptus leaves, calendula flowers, chamomile flowers, licorice roots and the terrestrial part of the scapularis in the following ratio of components, wt. %:

Propolis fifteen

Sage herb twenty

Eucalyptus leaves twenty Marigold flowers 10 Chamomile flowers 10 Licorice roots fifteen Ground part of shrubby cinquefoil 10

The technical essence of the present invention lies in the fact that it contains arabinogalactan, the combination of properties of which allows its use as a matrix for complex preparations and at the same time as an active component.

An aqueous-propylene glycol mixture was used as the optimal basis, which would combine good rheological properties and a universal extractant for propolis, medicinal plants, as well as an arabinogalactan solvent, which determines the content of both aqueous and fat-soluble biologically active substances in extracts and this has a low irritating effect.

Arabinogalactan is a polysaccharide of plant origin, it is obtained from larch wood, has high biological activity (immunomodulating, prebiotic, hypolipidemic, mitogenic, antimutagenic, hepatoprotective, gastroprotective, membrane-active, etc.). It is also characterized by a complex of other extremely valuable properties (low toxicity, good solubility in cold water, uniquely low viscosity of concentrated aqueous solutions). The combination of these properties allows the use of arabinogalactan as a matrix for complex drugs and at the same time as an active component of these drugs.

The bischofite mineral is magnesium chloride hexahydrate of the formula MgCl ₂ × 6H ₂ O, its deposits are found in vast areas of the Caspian Basin and the Volga monocline at depths of 1000-2000 m. Preparations from bischofite have a local effect and have anti-inflammatory, antimicrobial and fungistatic effects of varying degrees normalize microcirculation and metabolism.

Dimethyl sulfoxide or DMSO is a drug that has anti-inflammatory, analgesic effects. The drug has an antiseptic as well as fibrinolytic effect.

Dimethyl sulfoxide penetrates the skin and mucous membranes, it helps to increase the permeability of other drugs. DMSO is included in the class of low toxic drugs.

Excretion of the drug is performed by the kidneys in the form of dimethyl sulfone or dimethyl sulfate. May also stand out with air that is exhaled.

When determining the ratio of components in the preparation, we proceeded from the fact that each of the components affects the numerous links of the inflammatory syndrome, providing, to varying degrees, the antimicrobial and anti-inflammatory activity of the drug. At the same time, the main effect (antimicrobial and anti-inflammatory activity) of the drug is achieved by the inclusion of propolis, bischofite, sage grass, eucalyptus leaves and calendula flowers, chamomile flowers, licorice roots, and terrestrial shrubbery.

The chemical composition of the plants used is known that the proposed composition of plant materials contains: flavonoids (rutin, quercetin, avicularin, hyperoside, yuglanin, etc.), vitamins, carotenoids, triterpenoids, tannins, essential oils (chamazulene), organic acids, coumarins , silicic acid compounds, phenol glycoside, arbutin and other biologically active substances.

Preparation of the drug was carried out as follows (figure 1).

Propolis was cleaned of mechanical impurities, then it was crushed into pieces no larger than 20 mm in the freezer of a household refrigerator to a temperature of -10 ° C for at least 3 hours to completely freeze it and crushed in an M-8M mill to a powder state with particles no more than 2-4 mm. Propolis, crushed into fractions of 2-4 mm, has a large surface for extraction, which increases the extraction of biologically active substances, such as flavonoids, phytocides, unsaturated fatty acids (decenes), resins, essential oils and others. Medicinal plants: sage grass, eucalyptus leaves, calendula flowers, chamomile flowers, licorice roots and the aerial part of shrubby cinquefoil (each separately) were crushed in an MPR-2 mill to particles no more than 2-4 mm in size, mixed with propolis in a ratio of components wt. %: propolis - 15, sage grass - 20, eucalyptus leaves - 20, calendula flowers - 10, chamomile flowers - 10, licorice roots - 15, terrestrial shrubby cinquefoil - 10.

Then, the propolis-herbal mixture was placed in an ultrasonic unit "UZV-5.7 / 1 TTZ" and poured with a water-propylene glycol mixture (the ratio of water to propylene glycol 1: 1) in a mass ratio of 1:10. The resulting mixture was treated in a cavitation mode for 3.0 hours. The processing was carried out at the operating parameters of the installation (power consumption 460 W., frequency of ultrasonic vibrations 35 kHz and ultrasonic power 200 watts), with a gradual increase in temperature to 55 ° C, without access to light. Next, bischofite was added to the solution with working ultrasound in an amount of 20% of the amount of extractant, and then arabinogalactan was added in an amount of 20% by weight of the extractant. After complete dissolution of arabinogalactan, the resulting mixture was cooled and filtered by centrifugation at 3000 thousand rpm for 10 minutes. After that, dimethyl sulfoxide was added in an amount of 15% by weight of the finished product. The obtained water-propylene glycol extract, which after adding arabinogalactan acquires a gel-like consistency, was packaged in a clean, dry, odorless dish.

The resulting complex environmentally friendly anti-mastitis drug is a homogeneous mass, gel-like consistency, light brown in color, with a characteristic smell of propolis and medicinal plants.

The studies were conducted in 2016, in the Veterinary Laboratory of the Federal State Budget Scientific Institution Altai Research Institute of Livestock and Veterinary Medicine, the Scientific and Educational Complex Siberian Branch of the Russian Academy of Sciences - Altai State Medical University and the Federal State Unitary Enterprise Komsomolskoye Tribal Plant of the Pavlovsky District Altai Territory.

The object of the study were prototypes of the proposed anti-mastitis drug in the form of a gel.

In preclinical studies, 40 nonlinear white mongrel mice weighing 18-22 g, 20 Wistar rats weighing 200-250 and 5 cows weighing 500-550 kg 3-4 lactation, black-motley breed were used.

To assess the organoleptic properties of the drug, appearance, smell, and consistency were subjectively determined.

Thermostability was studied by heating 10.0 grams of an experimental sample of the drug in a well-closed tube in an thermostat at 37 ± 1 ° С for a day (24 hours) and by freezing a sample of the drug in a tube to - 20 ° С and subsequent gradual thawing at room temperature.

A gelatin gel diffusion method was used for invitro biopharmaceutical testing. For this purpose, 100 ml of a 2% solution of gelatin in a standard solvent (purified water) was prepared with the addition of an indicator - a 3% solution of iron chloride (III) in an amount of 1 ml. The hot solution was poured into Petri dishes and cooled. In the formed gel, 24 hours later, holes were cut out with a hollow metal cylinder with a diameter of 8 mm. 0.2 g were added to the resulting wells. test samples of the dosage form. The degree of release of the drug from the dosage form was evaluated by the diameter of the stained zone after 24 hours.

In order to determine the acute toxicity of the proposed drug were formed 4 groups of white mice weighing 21-24 g of 8 animals each. The first experimental group of laboratory animals was intragastrically injected with the finished dosage form of the drug at a dose of 15 ml, the second experimental group at a dose of 30 ml, and the third group at a dose of 45 ml, per kg, of body weight. The animals in the control group of mice were injected with an equivolume amount of saline. The clinical picture of "acute" poisoning was recorded after administration of the studied drug in various doses during the day. The duration of observation of animals was 14 days. During the study, integral indicators of animal health were recorded: external state, weight gain (weekly). At the end of the experiment, individual animals (5 animals from each group) were euthanized by decapitation and a diagnostic autopsy was performed to study the macroscopic state of internal organs. Weighing live weight of animals and internal organs was carried out on electronic scales. The calculation of mass coefficients was carried out according to the formula: MK = Organ mass (g) / body weight (g) * 100%.

The study of the local irritating effect of the proposed drug on the udder parenchyma was studied by the method of L.P. Malanina (1988). The content of somatic cells in milk, the increase in the number of which indicates an inflammatory reaction in the mammary gland, was determined as an indicator of the possible irritating effect of the drug. The effect of the drug on breast tissue was judged by the presence of a local inflammatory reaction, the change in the content of somatic cells in milk in terms: before administration and after 3, 6, 12, 24 and 48 hours after administration of the drug. The content of somatic cells in milk was determined on a Somatos viscometer analyzer (Russia) (GOST 54077-2010). At the same time, the irritating effect of the test drug was evaluated by clinical examination of the mammary gland of all experimental animals.

Anti-inflammatory activity was investigated on a model of acute inflammation after a weekly administration of an experimental preparation in the form of applications. The studies were carried out on 20 Wistar rats of both sexes weighing 200-250 g, which were divided into 2 groups of 10 animals each. Animals of the control group for 7 days received an equivolume amount of purified water. The last application of the experimental drug was carried out 1 hour before the injection of phlogistics. Acute exudative inflammation was induced by subplanetary administration of 0.1 ml of a 1% carrageenin solution. A clinical examination of each animal was carried out once a day. A detailed examination of the animal was performed in the cage and in the hands.

The measurement of the volume of the right hind limb was carried out using a digital plethysmometer “DigitalPletismometerLE7500” from Pan-labHARVARDapparatus, after a course of the experimental preparation before administration, and also 60, 120, and 240 min after phlogistic injection.

Based on the data of the average increase in the volume of the limbs of animals, the degree of anti-inflammatory activity (X) was calculated by the formula

Vк - average increase in limb volume in the control, cm ³ ;

Vo is the average increase in limb volume in the experiment, cm ³ .

The antipyretic effect of the drug was determined by its ability to lower the temperature of the surface of the rat body. Skin temperature was measured by an electronic thermometer (A&D medical, Japan) on the surface of the right hind limb.

Next, stability studies of complex preparation samples were performed. Their thermo- and colloidal stability were determined, parameters allowing predicting stability during the production and storage during changes in temperature parameters and mechanical stress.

Colloidal stability or delamination resistance of the preparation was determined by centrifugation. Stable was considered a system which, when centrifuged for 10 min at a speed of 4000 rpm, did not delaminate. The results are presented in table 1.

As follows from the data presented in table 3, the proposed drug withstands quite well both freezing and heating in a thermostat. The experimental sample was resistant to centrifugation, that is, it showed high colloidal stability.

Assessment of the degree of release of biologically active substances was carried out according to the traditional method of diffusion into the gel. The degree of release of the drug from the dosage form was evaluated by the diameter of the stained area. An experimental sample of a complex environmentally friendly preparation has a fairly high bioavailability, the diameter of the stained zone averaged 15-16 mm.

The study of acute toxicity shows the harmful effect of the drug, manifested after its single use. In preliminary tests to establish the maximum and minimum doses of the drug, 5 groups of animals with 3 heads in each were used, two of which were injected with a diluted 1: 2 and 1: 3 drug, while there were no clinical symptoms of poisoning.

After a single injection of the drug, mice showed slight excitement, they began to run around the cell, and then after 2-3 minutes an inhibited state occurred. The animals reacted poorly to external stimuli, there was a slight decrease in tactile and pain sensitivity. They sat hunched over, eyes closed, abdominal rapid breathing was noted. After 15-20 minutes, they began to slowly move around the cell, staggering from side to side. Over the next 20-30 minutes, most mice had manege movements, they periodically stopped and died. In the first two experimental groups, animal death was not observed. The signs of intoxication in the first group of 5 animals disappeared after 60 minutes, they began to actively move around the cage, climb the grate and gnaw food. In the second group, by this time, motor activity was restored in 3 mice, in 4 mice, weak activity was observed, one mouse lay hunched over and closed its eyes, activity in it appeared after 3 hours. In the third experimental group, the death of two mice. At autopsy, an edema of the walls of the abdominal cavity was found in one mouse, the stomach was dilated with gases, it contains drug residues and fodder masses, the mucous membrane is hyperemic. Another animal revealed hyperemia of the mucous membrane of the small intestine.

Table 2 shows the results on the dynamics of the live weight of mice for 14 days. Based on the above data, the dynamics of the mass of laboratory animals has differences between groups. In the control group there was the largest increase in body weight of mice by 13.1% (p <0.05) a week after administration of the drug, while in the first, second and third experimental groups there was an increase in animals by 11.5; 6.7 and 4.3%, respectively. On the 14th day, the smallest increase in live weight was detected in the third experimental group by 2.1%, while in the remaining groups the dynamics of the mass of mice was 5.4; 4.9 and 7.6%.

At the end of the experiment, after 14 days, during an external examination of laboratory animals, no deviations in physique, deformation, and swelling of the extremities were observed. The coat was even, smooth, the skin without scratching and ulceration.

Note: * - p <0.05

At autopsy there were no violations in the location, shape and structure of internal organs. Visually, differences in the size of parenchymal organs (liver, spleen) between the animals of the control and experimental groups were noted. Moreover, in experimental animals of the first, second and third experimental groups revealed an increase in the size of the spleen in 1.44; 1.85 and 1.77 times, the size of the liver was 17 more; 4.4 and 8.1%, respectively, in comparison with the organs of animals in the control group.

Mass coefficient (MK) is the percentage of organ mass to body mass, an integral indicator used in toxicology to assess the condition of internal organs. The analysis of this indicator in toxicological studies makes it possible to detect the target organ of a toxicant and to reveal signs of endocrine-related effects. Data on the weights of the organs of mice are shown in table 3.

The introduction of the proposed drug contributed to a smaller increase in experimental animals and an increase in the weight coefficients of parenchymal organs in comparison with animals of the control group, especially the weight coefficients of the spleen and had dose-dependent character.

Studies of the irritating effect of the proposed drug was carried out on five clinically healthy cows. The drug was administered intracisternally in the left lobes of the udder at a dose of 10 ml, the right lobes served as a control (0.85% aqueous NaCl solution was introduced). After administration of the drug at 6, 12, 24 and 48 hours, the state of the udder was taken into account, and milk samples were taken to calculate the number of somatic cells. It was found that within 48 hours after administration of the drug, the general condition of the studied animals was within physiological boundaries. The local temperature of the experimental and control quarters of the mammary gland was not increased; the skin of the udder is elastic, painless.

When studying milk samples for the content of somatic cells, a certain dynamics of changes in the level of somatic cells in the milk of cows of the experimental group was noted. Their insignificant decrease was noted (to 371 ± 29.3 thousand / ml) 6 hours after drug administration, and 12 hours after drug administration the number of somatic cells decreased (to 206 ± 33.8 thousand / ml) and after 24 hours the number of somatic cells increased to (385 ± 48.8 thousand / ml). The restoration of the initial level of somatic cells occurred after 48 hours and was within the physiological norm (table. 4).

Note: * - p <0.05

Counting somatic cells in milk from control quarters showed insignificant fluctuations in somatic cells with a decrease in their number in the first 6 hours after the injection of saline, and a gradual increase by 48 hours, however, these changes in the level of somatic cells were not reliable.

Data may indicate normal physiological fluctuations in the amount of SC in milk. The results of the experiment indicate that the drug did not irritate the parenchyma of the udder of cows.

The dynamics of the volume of the affected limb was evaluated by the oncometric method. For statistical data processing, nonparametric statistical methods were used. The median (M) and quarterly range (QUART) were calculated. As you know, a change in volume during the inflammatory process against the background of the use of drugs characterizes the antiexudative effect of the latter. The data obtained are shown in table 5.

When evaluating the data, it is clear that subplantar administration of carrageenin to the control group of animals led to the rapid formation of limb edema. Already 60 minutes after the phlogistic injection, the paw volume exceeded the initial level by an average of 0.49 cm ³ , which corresponds to a 42.1% increase in volume (p <0.05) compared with the initial indicator.

Note: * - p <0.05

Subsequently, the volume of the limb gradually increased, reaching its maximum value by the fourth hour of observation. By this time, the increase was 0.67 cm, which corresponds to an almost double increase in limb volume (p <0.05).

Under conditions of prolonged administration, the studied drug weakened the formation of edema - 35.91%.

Thus, in conditions of prophylactic application of the studied drug to animals at a dose of 0.25 ml, its effect on the dynamics of the development of acute inflammatory edema caused by carrageenin is noted, which indicates effectiveness in the acute inflammatory process.

The effect of the drug on the local temperature of the damaged limb (antipyretic effect)

The dynamics of the local temperature of the injured limb was carried out by the contact method using an infrared thermometer. The data are shown in table 6.

Note: * - p <0.05

1 hour after the induction of arthritis in male rats in the Control group, a statistically significant increase in local limb temperature by 15% was observed. This effect persisted until the 4th hour of the experiment.

The test drug already 2 hours after application showed an antipyretic effect, which was reflected in a statistically significant decrease in the temperature of the damaged limb by 7.8%.

The antimicrobial properties of the drug were tested by agar diffusion.

As test cultures, daily field isolates of microorganisms isolated from the milk of patients with mastitis cows were used. The results of the studies are shown in the table.

Analyzing the results of studies to determine the sensitivity of opportunistic microflora to the proposed drug, it can be noted that the drug exhibits pronounced antimicrobial activity in relation to the studied strains.

To determine the optimal therapeutic dose of the drug according to the principle of analogues, three groups of black-motley cows with acute catarrhal mastitis were formed. Sick cows were injected intracisternally with the proposed drug, at a dose of 10, 15 and 20 ml, twice a day, against the background of symptomatic therapy.

The use of the drug in a dose of 10 ml showed the smallest therapeutic effect. Recovery on average occurred on 6.5-7.2 days, the percentage of recovery was 60%.

The use of the drug at a dose of 15 and 20 ml showed the greatest therapeutic effect and is characterized by similar indicators of both the timing of recovery and the percentage of recovered animals (Table 8).

As a result of data analysis to determine the optimal therapeutic dose, it was found that the greatest therapeutic effect was achieved when using the drug at a dose of 15 ml with a frequency of administration of 2 times a day. The use of the drug in a dose of 20 ml did not show a significant increase in therapeutic effectiveness and led to an overuse of the drug.

The research results showed that the most effective dose of the drug, which has a pronounced therapeutic effect, is a dose of 15 ml.

To study the comparative effectiveness of cows mastitis therapy according to the analogue principle, two groups of animals with acute catarrhal mastitis were formed: the first group (n = 15) was injected with mastomycin (containing 0.015 g of gentamicin sulfate, 0.020 g of clindamycin hydrochloride and 0.05 g of lidocaine as active substances hydrochloride, as adjuvants poloxamer, glycerin, benzyl alcohol, sodium hydroxide and distilled water) intracisternally at a dose of 10 ml, 2 times a day; the second group (n = 17) was administered the proposed drug in a dose of 15 ml, 2 times a day, until recovery.

The therapeutic efficacy of the proposed drug was determined by the number of treatment days, the duration of which was determined by the negative Kenotest reaction, the percentage of recovered animals and the clinical condition of patients with breast fractions.

The results obtained in the treatment of catarrhal form of mastitis in cows during lactation are presented in table 9.

The data in the table indicate that in the first experimental group, where Mastomycin was used, there was a recovery in 12 (80.0%) and improvement in 3 animals (20.0%). The duration of treatment averaged 6.7 days. At the same time, milk from these cows was disposed of within three days, according to the instructions for use of the drug.

In the group of animals where the proposed drug was used, recovery occurred in 14 animals (82.0%). The duration of treatment averaged 5.8 days. During the course of treatment, the general condition of the animals improved, signs of inflammation, swelling and soreness of the affected quarters decreased. The appearance of the secretion from the cured quarters did not differ from the milk of healthy cows, and its study for the presence of mastitis with a kenotest yielded a negative result.

Thus, the proposed method for the treatment of acute catarrhal mastitis using the proposed complex anti-mastitis drug, with an intracisternal route of administration at a dose of 15 ml, 2 times a day, is more acceptable, in comparison with mastitis therapy with the complex antibiotic drug Mastomycin, which was expressed in a decrease in the treatment time and a slight increase in the number of recovered animals, as well as a reduction in the utilization of milk.

Claims (4)

A drug for the treatment of cows with mastitis, including propolis extract and herbal extract, characterized in that it additionally contains arabinogalactan, bischofite, dimethyl sulfoxide, and an aqueous-propylene glycol mixture in the ratio of water and propylene glycol 1 as an extractant for propolis and grass collection: 1, in the following ratio of components, wt.%: Arabinogalactan twenty Bischofite twenty Dimethyl sulfoxide fifteen 10% water-propylene glycol extract mixtures of propolis and herbal rest, where the mixture of propolis and grass collection contains propolis, sage grass, eucalyptus leaves, calendula flowers, chamomile flowers, licorice roots and the terrestrial part of the scapula in the following ratio, wt.%: Propolis fifteen Sage herb twenty Eucalyptus leaves twenty Marigold flowers 10 Chamomile flowers 10 Licorice roots fifteen Ground part of shrubby cinquefoil 10

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