CN107474356A - Polyethylene micro-ecological agricultural plastic master batch and seedling raising tray made of same - Google Patents
Polyethylene micro-ecological agricultural plastic master batch and seedling raising tray made of same Download PDFInfo
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- CN107474356A CN107474356A CN201710667568.2A CN201710667568A CN107474356A CN 107474356 A CN107474356 A CN 107474356A CN 201710667568 A CN201710667568 A CN 201710667568A CN 107474356 A CN107474356 A CN 107474356A
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- polyethylene
- bacillus
- seedling
- plastic matrix
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- -1 Polyethylene Polymers 0.000 title claims abstract description 52
- 239000004698 Polyethylene Substances 0.000 title claims abstract description 51
- 229920000573 polyethylene Polymers 0.000 title claims abstract description 51
- 229920003023 plastic Polymers 0.000 title claims abstract description 46
- 239000004033 plastic Substances 0.000 title claims abstract description 46
- 239000004594 Masterbatch (MB) Substances 0.000 title abstract description 7
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 34
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000005995 Aluminium silicate Substances 0.000 claims abstract description 21
- 235000012211 aluminium silicate Nutrition 0.000 claims abstract description 21
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 17
- 239000006041 probiotic Substances 0.000 claims abstract description 17
- 235000018291 probiotics Nutrition 0.000 claims abstract description 17
- 239000000440 bentonite Substances 0.000 claims abstract description 16
- 229910000278 bentonite Inorganic materials 0.000 claims abstract description 16
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000000314 lubricant Substances 0.000 claims abstract description 8
- 239000003381 stabilizer Substances 0.000 claims abstract description 8
- 239000003054 catalyst Substances 0.000 claims abstract description 7
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 66
- 239000011159 matrix material Substances 0.000 claims description 40
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 33
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 28
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 claims description 22
- 238000000855 fermentation Methods 0.000 claims description 20
- 230000004151 fermentation Effects 0.000 claims description 20
- 239000000463 material Substances 0.000 claims description 19
- 238000002360 preparation method Methods 0.000 claims description 15
- 241000894006 Bacteria Species 0.000 claims description 14
- 241000193403 Clostridium Species 0.000 claims description 14
- 239000004310 lactic acid Substances 0.000 claims description 14
- 235000014655 lactic acid Nutrition 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 13
- 244000063299 Bacillus subtilis Species 0.000 claims description 11
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 11
- 238000010899 nucleation Methods 0.000 claims description 11
- 230000010355 oscillation Effects 0.000 claims description 11
- 241000193417 Brevibacillus laterosporus Species 0.000 claims description 10
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 10
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 9
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 9
- 239000002689 soil Substances 0.000 claims description 9
- 238000005119 centrifugation Methods 0.000 claims description 8
- 238000001125 extrusion Methods 0.000 claims description 7
- 239000002245 particle Substances 0.000 claims description 7
- 241000194108 Bacillus licheniformis Species 0.000 claims description 6
- YCIGYTFKOXGYTA-UHFFFAOYSA-N 4-(3-cyanopropyldiazenyl)butanenitrile Chemical group N#CCCCN=NCCCC#N YCIGYTFKOXGYTA-UHFFFAOYSA-N 0.000 claims description 5
- FYHXNYLLNIKZMR-UHFFFAOYSA-N calcium;carbonic acid Chemical compound [Ca].OC(O)=O FYHXNYLLNIKZMR-UHFFFAOYSA-N 0.000 claims description 5
- YQHLDYVWEZKEOX-UHFFFAOYSA-N cumene hydroperoxide Chemical compound OOC(C)(C)C1=CC=CC=C1 YQHLDYVWEZKEOX-UHFFFAOYSA-N 0.000 claims description 5
- 238000007664 blowing Methods 0.000 claims description 4
- 230000003185 calcium uptake Effects 0.000 claims description 4
- 238000000227 grinding Methods 0.000 claims description 4
- 238000005453 pelletization Methods 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 238000007493 shaping process Methods 0.000 claims description 4
- 150000001298 alcohols Chemical class 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- 108700026027 Bacteria Vat Proteins 0.000 claims description 2
- 239000012071 phase Substances 0.000 claims description 2
- 235000013406 prebiotics Nutrition 0.000 claims description 2
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims 1
- 229910052622 kaolinite Inorganic materials 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 12
- 238000000034 method Methods 0.000 abstract description 12
- 230000012010 growth Effects 0.000 abstract description 10
- 230000004083 survival effect Effects 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 239000000575 pesticide Substances 0.000 abstract description 4
- 230000035784 germination Effects 0.000 abstract description 3
- 238000012856 packing Methods 0.000 abstract description 3
- 238000004321 preservation Methods 0.000 abstract 2
- 208000035240 Disease Resistance Diseases 0.000 abstract 1
- 230000002262 irrigation Effects 0.000 abstract 1
- 238000003973 irrigation Methods 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 41
- 235000007164 Oryza sativa Nutrition 0.000 description 41
- 235000009566 rice Nutrition 0.000 description 41
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 22
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 14
- 240000008042 Zea mays Species 0.000 description 14
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 14
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 14
- 235000005822 corn Nutrition 0.000 description 14
- 239000002609 medium Substances 0.000 description 14
- 230000001954 sterilising effect Effects 0.000 description 14
- 241000193744 Bacillus amyloliquefaciens Species 0.000 description 11
- 241000196324 Embryophyta Species 0.000 description 10
- 241000726221 Gemma Species 0.000 description 10
- 238000004519 manufacturing process Methods 0.000 description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 244000046052 Phaseolus vulgaris Species 0.000 description 7
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 7
- 238000003556 assay Methods 0.000 description 7
- 235000015278 beef Nutrition 0.000 description 7
- 229910052799 carbon Inorganic materials 0.000 description 7
- 239000000284 extract Substances 0.000 description 7
- 235000013312 flour Nutrition 0.000 description 7
- 239000011521 glass Substances 0.000 description 7
- 238000011534 incubation Methods 0.000 description 7
- 239000006916 nutrient agar Substances 0.000 description 7
- 239000011780 sodium chloride Substances 0.000 description 7
- 238000004659 sterilization and disinfection Methods 0.000 description 7
- 238000012549 training Methods 0.000 description 7
- 206010042674 Swelling Diseases 0.000 description 6
- 230000002786 root growth Effects 0.000 description 6
- 230000008961 swelling Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 239000003337 fertilizer Substances 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 244000025254 Cannabis sativa Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000012935 Averaging Methods 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000010353 tongjiang Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L23/00—Compositions of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Compositions of derivatives of such polymers
- C08L23/02—Compositions of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Compositions of derivatives of such polymers not modified by chemical after-treatment
- C08L23/04—Homopolymers or copolymers of ethene
- C08L23/06—Polyethene
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0293—Seed or shoot receptacles
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/20—Compounding polymers with additives, e.g. colouring
- C08J3/22—Compounding polymers with additives, e.g. colouring using masterbatch techniques
- C08J3/226—Compounding polymers with additives, e.g. colouring using masterbatch techniques using a polymer as a carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2327/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a halogen; Derivatives of such polymers
- C08J2327/02—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a halogen; Derivatives of such polymers not modified by chemical after-treatment
- C08J2327/04—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a halogen; Derivatives of such polymers not modified by chemical after-treatment containing chlorine atoms
- C08J2327/06—Homopolymers or copolymers of vinyl chloride
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2423/00—Characterised by the use of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Derivatives of such polymers
- C08J2423/02—Characterised by the use of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Derivatives of such polymers not modified by chemical after treatment
- C08J2423/04—Homopolymers or copolymers of ethene
- C08J2423/08—Copolymers of ethene
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/18—Oxygen-containing compounds, e.g. metal carbonyls
- C08K3/24—Acids; Salts thereof
- C08K3/26—Carbonates; Bicarbonates
- C08K2003/265—Calcium, strontium or barium carbonate
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K2201/00—Specific properties of additives
- C08K2201/011—Nanostructured additives
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K2201/00—Specific properties of additives
- C08K2201/014—Additives containing two or more different additives of the same subgroup in C08K
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a polyethylene micro-ecological agricultural plastic master batch and a seedling raising tray prepared from the master batch, wherein the master batch comprises the following components in parts by weight: 20-50 parts of polyethylene, 10-20 parts of bentonite, 10-30 parts of kaolin, 10-60 parts of calcium carbonate, 0.1-1 part of catalyst, 0.1-5 parts of compatilizer, 0.5-2 parts of stabilizer, 0.5-5 parts of lubricant and 0.5-5 parts of mixed probiotics. The seedling raising tray prepared from the polyethylene micro-ecological agricultural plastic master batch is well formed, and the added nano-scale bentonite, kaolin and calcium carbonate have the effects of moisture preservation and heat preservation, are beneficial to seedling germination and reduce the labor intensity of irrigation; the probiotics beneficial to the growth of the seedlings are added, so that the seedlings have certain disease resistance, the roots of the seedlings have more roots, the packing effect is good, the stems are thick and strong, the emergence rate and the survival rate are high, the fertilizing amount and the pesticide amount in the seedling raising process are reduced, and the labor force is greatly reduced.
Description
Technical field
The present invention relates to agricultural to use plastic matrix field, in particular it relates to the agricultural of the polyethylene Tiny ecosystem containing profitable probliotics
Plastic matrix and the seedling-cultivation plate being made from it.
Background technology
Existing raising rice seedlings technology mainly carries out seedling using seedling-cultivation plate, after rice shoot length to a certain extent after, then by seedling
Disk is transplanted to field.Seedling is an important process, and seedling process rice shoot grows, then is advantageous to the growth in rice shoot later stage.
The seedling-cultivation plate that seedling process uses also has a certain impact to bud ratio, the upgrowth situation of rice shoot, therefore by improving seedling-cultivation plate
Situation, the growing way of seedling process rice shoot can be improved, improve the bud ratio of rice shoot.
At present, agriculturally usually used seedling-cultivation plate, in use not environmentally, be difficult to recycle, easily cause
Environmental pollution.In addition, the service life of seedling-cultivation plate is short, substantially it is used only once, Quality Down when reusing for the second time, reaches
Less than the requirement of seedling.Conventional seedling-cultivation plate is only used for temporarily cultivating rice shoot, it is impossible to other nutrients are provided for rice shoot developmental process,
Therefore periodically fertilising, spraying insecticide and agricultural chemicals are also needed in seedling process, not only increases labour, can yet influence rice shoot
Emergence rate and survival rate and emergence quality etc..
Therefore, it is necessary to which a kind of easily reclaim, be environmentally friendly, will not produce secondary pollution, while and can improves the bud ratio of rice shoot
And survival rate, reduce seedling raising process in fertilizer amount and pesticide volume seedling-cultivation plate.
The content of the invention
A variety of probiotics are added into polyethylene and the plastic matrix containing profitable probliotics are made by the present invention, are reprocessed into seedling
Disk.
The technical problems to be solved by the invention are to be directed to above-mentioned the deficiencies in the prior art, there is provided one kind contains profitable probliotics
Plastic matrix, it is recyclable plastic products, will not cause secondary pollution, the seedling-cultivation plate being made from it can improve seedling
The bud ratios and survival rate of seedling, dose and pesticide volume in seedling raising process are reduced, both can guarantee that the healthy growth of seedling raising,
Onerous toil power can be reduced.
In order to solve the above technical problems, the technical solution adopted by the present invention is：A kind of polyethylene Tiny ecosystem agricultural plastic master
Material, include the component of following parts by weight：20-50 parts polyethylene, 10-20 parts bentonite, 10-30 parts kaolin, 10-60 part carbonic acid
Calcium, 0.1-1 parts catalyst, 0.1-5 parts compatilizer, 0.5-2 parts stabilizer and 0.5-5 parts lubricant and the mixing of 0.5-5 parts are prebiotic
Bacterium.
Preferably, the mixing probiotics includes the strain of following mass percent：55-60% clostridium, 25-
30% bar-shaped bacillus and 10-16% lactic acid bacillus.
Preferably, the clostridium includes the strain of following mass percent：18-25% bacillus licheniformis,
35-45% bacillus subtilises and 37-42% bacillus amyloliquefaciens.
The bacillus subtilis and bacillus amyloliquefaciens of the present invention can be known, such as Chinese microorganism strain collection
, deposit number is CGMCC NO.13643 bacillus amyloliquefaciens, and deposit number is CGMCC NO.13642 withered grass bud
Spore bar.
Bacillus includes the strain of following mass percent：30-40% bacillus laterosporuses and 60-70% Su Yun gold gemma bars
Bacterium.
Preferably, the lactic acid bacillus includes the strain of following mass percent：10-20% butyric acid bacillus and
80-90% acetic acid bacillus.
The bacterial strain of the present invention can be known, for example, the lichens provided by Guangdong Huizhou Li Bo bio tech ltd
The Soviet Union that bacillus, the bacillus laterosporus of Henan Yikang biology Co., Ltd offer, Guangzhou Ba Nong biotechnologies company provide
The butyric acid bacillus and acetic acid bacillus that cloud gold bacillus, Shandong Su Kehan bioengineering limited company provide.
Preferably, the catalyst is azo dibutyronitrile or isopropyl benzene hydroperoxide；The compatilizer is EVA or PEO；Institute
It is organic tin to state stabilizer；The lubricant is higher alcohols.
Described organic tin is tin mercaptides, and higher alcohols are 16 carbon alcohol.
Preferably, the particle diameter of the bentonite, kaolin and calcium carbonate is nanoscale.Add the swelling of Nano Particle
Soil, kaolin and calcium carbonate, these materials have moisturizing, water suction and heat insulation effect, are advantageous to improve the germination percentage of rice shoot, mitigate
The labor intensity of pouring.
The invention provides a kind of preparation method of polyethylene Tiny ecosystem agricultural plastic matrix, comprise the following steps：
1) each pure culture is distinguished into oscillation and fermentation cultivation；
2) each pure culture after fermented culture in step 1) is centrifuged；
3) each pure culture that centrifugation obtains in step 2) is put into seeding tank, is enlarged culture；
4) each pure culture that step 3) expansion culture obtains is put into bacteria vat and carries out fermented and cultured；
5) each pure culture for obtaining step 4) fermented and cultured centrifuges；
6) each pure culture that centrifugation obtains in step 5) is mixed, obtains Mixed Microbes；
7) Mixed Microbes are mixed with bentonite, kaolin, carbonic acid calcium uptake, dries, crushes, grinding turns into Mixed Microbes powder
Body；
8) polyethylene, catalyst, phase solvent, stabilizer and lubricant are added into successively in reactor, at 80-120 DEG C
At a temperature of, 1-5 hours are stirred, blowing is cooled down, obtains mixed material；
9) mixed material for obtaining step 8) is put into granulating extruder, extruding pelletization, obtains granular polyethylene
Tiny ecosystem agricultural plastic matrix.
Specifically, the culture of various bacterium is as follows：
1. the culture of bacillus licheniformis：
Strain, which is fetched, from Guangdong Huizhou Li Bo bio tech ltd expands culture.
Incubation equipment and instrument：One, high-temperature sterilization pot；One, constant temperature water tower/set；Tube centrifuge one/set；
Ultra-clean sterilizing cabinet one/set；THZ-92A Desk type constant-temperatureoscillator oscillators (oscillation amplitude 25mm) one/set；One, microscope；Culture
Ware, glass apparatus are a collection of.
Cultivation and production process：Slant medium：Beef extract：0.2-1.0%；Albumen freezes：0.5-1.0%；Nutrient agar：
0.5-1.0%；NaCl：0.1-0.5%, surplus are water.
Fermentation medium：Dregs of beans：1-5%；Corn flour：1-3%；Corn steep liquor：0.2-1.0%；KHPO4：0.1-0.3%；
Surplus is water.
Fermented and cultured：Using 500ml triangular flasks, built-in 50ml fermentation mediums, training is vibrated under 30-80 DEG C of 200r/min
Support 10-40h.
Strain after fermented and cultured is separated through centrifuge, into 100L seeding tanks, expands culture 8- at 40-80 DEG C
12 hours, subsequently enter 1000L bacteria vats and carry out 24-48 hours of fermenting, after the assay was approved, separated into centrifuge, obtain ground
Clothing bacillus.
2. the culture of bacillus subtilis：
The bacillus subtilis that deposit number is CGMCC NO.13642, which is fetched, from collection expands culture.
Incubation equipment and instrument：One, high-temperature sterilization pot；One, constant temperature water tower/set；Tube centrifuge one/set；
Ultra-clean sterilizing cabinet one/set；THZ-92A Desk type constant-temperatureoscillator oscillators (oscillation amplitude 25mm) one/set；One, microscope；Culture
Ware, glass apparatus are a collection of.
Cultivation and production process：Slant medium：Beef extract：0.2-1.0%；Albumen freezes：0.5-1.0%；Nutrient agar：
0.5-1.0%；NaCl：0.1-0.5%, surplus are water.
Fermentation medium：Dregs of beans：1-5%；Corn flour：1-3%；Corn steep liquor：0.2-1.0%；KHPO4：0.1-0.3%；
Surplus is water.
Fermented and cultured：Using 500ml triangular flasks, built-in 50ml fermentation mediums, training is vibrated under 30-80 DEG C of 200r/min
Support 10-40h.
Strain after fermented and cultured is separated through centrifuge, into 100L seeding tanks, expands culture 8- at 40-80 DEG C
12 hours, subsequently enter 1000L bacteria vats and carry out 24-48 hours of fermenting, after the assay was approved, separate, obtain withered into centrifuge
Careless bacillus.
3. the culture of bacillus amyloliquefaciens：
The bacillus amyloliquefaciens that deposit number is CGMCC NO.13643, which are fetched, from collection expands culture.
Incubation equipment and instrument：One, high-temperature sterilization pot；One, constant temperature water tower/set；Tube centrifuge one/set；
Ultra-clean sterilizing cabinet one/set；THZ-92A Desk type constant-temperatureoscillator oscillators (oscillation amplitude 25mm) one/set；One, microscope；Culture
Ware, glass apparatus are a collection of.
Cultivation and production process：Slant medium：Beef extract：0.2-1.0%；Albumen freezes：0.5-1.0%；Nutrient agar：
0.5-1.0%；NaCl：0.1-0.5%, surplus are water.
Fermentation medium：Dregs of beans：1-5%；Corn flour：1-3%；Corn steep liquor：0.2-1.0%；KHPO4：0.1-0.3%；
Surplus is water.
Fermented and cultured：Using 500ml triangular flasks, built-in 50ml fermentation mediums, training is vibrated under 30-80 DEG C of 200r/min
Support 10-40h.
Strain after fermented and cultured is separated through centrifuge, into 100L seeding tanks, expands culture 8- at 40-80 DEG C
12 hours, subsequently enter 1000L bacteria vats and carry out 24-48 hours of fermenting, after the assay was approved, separate, solved into centrifuge
Bacillus amyloliquefacienses.
4. the culture of bacillus laterosporus：
The strain of fetching provided from Henan Yikang biology Co., Ltd expands culture.
Incubation equipment and instrument：One, high-temperature sterilization pot；One, constant temperature water tower/set；Tube centrifuge one/set；
Ultra-clean sterilizing cabinet one/set；THZ-92A Desk type constant-temperatureoscillator oscillators (oscillation amplitude 25mm) one/set；One, microscope；Culture
Ware, glass apparatus are a collection of.
Cultivation and production process：Slant medium：Beef extract：0.2-1.0%；Albumen freezes：0.5-1.0%；Nutrient agar：
0.5-1.0%；NaCl：0.1-0.5%, surplus are water.
Fermentation medium：Dregs of beans：1-5%；Corn flour：1-3%；Corn steep liquor：0.2-1.0%；KHPO4：0.1-0.3%；
Surplus is water.
Fermented and cultured：Using 500ml triangular flasks, built-in 50ml fermentation mediums, training is vibrated under 30-80 DEG C of 200r/min
Support 10-40h.
Strain after fermented and cultured is separated through centrifuge, into 100L seeding tanks, expands culture 8- at 40-80 DEG C
12 hours, subsequently enter 1000L bacteria vats and carry out 24-48 hours of fermenting, after the assay was approved, separated into centrifuge, obtain side
Spore bacillus.
5. the culture of bacillus thuringiensis：
From Guangzhou, strain expansion culture is fetched by Ba Nong biotechnologies company.
Incubation equipment and instrument：One, high-temperature sterilization pot；One, constant temperature water tower/set；Tube centrifuge one/set；
Ultra-clean sterilizing cabinet one/set；THZ-92A Desk type constant-temperatureoscillator oscillators (oscillation amplitude 25mm) one/set；One, microscope；Culture
Ware, glass apparatus are a collection of.
Cultivation and production process：Slant medium：Beef extract：0.2-1.0%；Albumen freezes：0.5-1.0%；Nutrient agar：
0.5-1.0%；NaCl：0.1-0.5%, surplus are water.
Fermentation medium：Dregs of beans：1-5%；Corn flour：1-3%；Corn steep liquor：0.2-1.0%；KHPO4：0.1-0.3%；
Surplus is water.
Fermented and cultured：Using 500ml triangular flasks, built-in 50ml fermentation mediums, training is vibrated under 30-80 DEG C of 200r/min
Support 10-40h.
Strain after fermented and cultured is separated through centrifuge, into 100L seeding tanks, expands culture 8- at 40-80 DEG C
12 hours, subsequently enter 1000L bacteria vats and carry out 24-48 hours of fermenting, after the assay was approved, separate, revived into centrifuge
Cloud gold bacillus.
6. the culture of butyric acid bacillus：
From Shandong, strain expansion culture is fetched by Su Kehan bioengineering limited company.
Incubation equipment and instrument：One, high-temperature sterilization pot；One, constant temperature water tower/set；Tube centrifuge one/set；
Ultra-clean sterilizing cabinet one/set；THZ-92A Desk type constant-temperatureoscillator oscillators (oscillation amplitude 25mm) one/set；One, microscope；Culture
Ware, glass apparatus are a collection of.
Cultivation and production process：Slant medium：Beef extract：0.2-1.0%；Albumen freezes：0.5-1.0%；Nutrient agar：
0.5-1.0%；NaCl：0.1-0.5%, surplus are water.
Fermentation medium：Dregs of beans：1-5%；Corn flour：1-3%；Corn steep liquor：0.2-1.0%；KHPO4：0.1-0.3%；
Surplus is water.
Fermented and cultured：Using 500ml triangular flasks, built-in 50ml fermentation mediums, training is vibrated under 30-80 DEG C of 200r/min
Support 10-40h.
Strain after fermented and cultured is separated through centrifuge, into 100L seeding tanks, expands culture 8- at 40-80 DEG C
12 hours, subsequently enter 1000L bacteria vats and carry out 24-48 hours of fermenting, after the assay was approved, separated into centrifuge, obtain fourth
Sour bacillus.
7. the culture of acetic acid bacillus：
From Shandong, strain expansion culture is fetched by Su Kehan bioengineering limited company.
Incubation equipment and instrument：One, high-temperature sterilization pot；One, constant temperature water tower/set；Tube centrifuge one/set；
Ultra-clean sterilizing cabinet one/set；THZ-92A Desk type constant-temperatureoscillator oscillators (oscillation amplitude 25mm) one/set；One, microscope；Culture
Ware, glass apparatus are a collection of.
Cultivation and production process：Slant medium：Beef extract：0.2-1.0%；Albumen freezes：0.5-1.0%；Nutrient agar：
0.5-1.0%；NaCl：0.1-0.5%, surplus are water.
Fermentation medium：Dregs of beans：1-5%；Corn flour：1-3%；Corn steep liquor：0.2-1.0%；KHPO4：0.1-0.3%；
Surplus is water.
Fermented and cultured：Using 500ml triangular flasks, built-in 50ml fermentation mediums, training is vibrated under 30-80 DEG C of 200r/min
Support 10-40h.
Strain after fermented and cultured is separated through centrifuge, into 100L seeding tanks, expands culture 8- at 40-80 DEG C
12 hours, subsequently enter 1000L bacteria vats and carry out 24-48 hours of fermenting, after the assay was approved, separated into centrifuge, obtain fourth
Sour bacillus.
The invention provides a kind of seedling-cultivation plate, the seedling-cultivation plate polyethylene Tiny ecosystem agricultural plastic matrix is through extrusion forming system
Into.
The invention provides the preparation method of seedling-cultivation plate, comprise the following steps：
1) polyethylene Tiny ecosystem agricultural plastic matrix being put into calender and carries out tabletting, temperature control is 150-210 DEG C,
Obtain sheet material；
2) sheet material obtained in step 1) is put into seedling disc shaping machine, temperature control is 130-150 DEG C, is squeezed into
Type, obtain seedling-cultivation plate.
The polyethylene Tiny ecosystem agricultural plastic matrix being prepared contains abundant probiotics can be directly as fertilizer
The root of crops is put into, the hardened situation of soil can be improved, increase the fertility of soil, supplement the fertilizer of crops, make agriculture
Crop grows vigorously, and rhizome is flourishing, improves the lodging tolerance of crops.
The beneficial effects of the invention are as follows：The present invention by the seedling-cultivation plate that polyethylene Tiny ecosystem agricultural plastic matrix is prepared into
Type is good, and being added into nano level bentonite, kaolin, calcium carbonate has moisturizing, heat insulation effect, is advantageous to rice shoot germination, mitigates
Pour labor intensity；The probiotics beneficial to seedling growth is added into, makes rice shoot that there is certain resistance against diseases, rice shoot root root
Must it is more, packing effect is good, stem is sturdy, emergence rate, survival rate height, reduce the dose and pesticide volume during seedling, significantly
Reduce labour.The Tiny ecosystem agricultural plastic matrix of preparation and the number of viable of seedling-cultivation plate are high, and Tiny ecosystem agricultural masterbatch survives
Bacterium number amount be 500-1000 hundred million/gram；Seedling-cultivation plate into number of viable be 1,000,000/gram more than.
Brief description of the drawings
Fig. 1 is the seedling growth growing way photo of 12 days that the seedling-cultivation plate prepared using the present invention is cultivated；
Fig. 2 is the seedling growth growing way photo of 12 days cultivated using conventional blanket disk；
Fig. 3 is the root growth feelings of the rice shoot for the identical plant height that blanket disk that the seedling-cultivation plate of the present invention is cultivated and conventional is cultivated
The photo of condition；
Fig. 4 is the seedling growth growing state of 15 days that blanket disk cultivated using the seedling-cultivation plate of the present invention and conventional is cultivated
Photo；
Fig. 5 is using the photo of the root growth situation before the shoot transplanting equipment of the seedling-cultivation plate of present invention cultivation.
Embodiment
Lead to embodiment below, technical scheme is described in further detail.
Embodiment 1
A kind of polyethylene Tiny ecosystem agricultural plastic matrix, include the component of following parts by weight：20 parts of polyethylene, 10 parts of swellings
Soil, 10 parts of kaolin, 10 parts of calcium carbonate, 0.1 part of azo dibutyronitrile, 0.1 part of EVA, 0.5 part of tin mercaptides, 0.5 part of 16 carbon alcohol and
0.5 part of mixing probiotics.Wherein, mixing probiotics includes the strain of following mass percent：55% clostridium, 30%
Bar-shaped bacillus and 15% lactic acid bacillus.
Wherein, clostridium includes the strain of following mass percent：18% bacillus licheniformis, 45% withered grass bud
Spore bacillus and 37% bacillus amyloliquefaciens.Bar-shaped bacillus includes the strain of following mass percent：30% side spore gemma
Bacillus and 70% bacillus thuringiensis.The lactic acid bacillus includes the strain of following mass percent：10% butyric acid bud
Spore bacillus and 90% acetic acid bacillus.The particle diameter of bentonite, kaolin and calcium carbonate is nanoscale.
The preparation method of polyethylene Tiny ecosystem agricultural plastic matrix, comprises the following steps：
1) each pure culture is distinguished into oscillation and fermentation cultivation；Using 500ml triangular flasks, built-in 50ml fermentation mediums, 30
DEG C, under 200r/min, shaken cultivation 40h, wherein, each pure culture is bacillus licheniformis, bacillus subtilis, solution starch respectively
Bacillus, bacillus laterosporus, bacillus thuringiensis, butyric acid bacillus or acetic acid bacillus；
2) each pure culture after fermented culture in step 1) is centrifuged；
3) each pure culture that centrifugation obtains in step 2) is put into 100L seeding tanks, culture 12 is enlarged at 40 DEG C
Hour；
4) each pure culture that step 3) expansion culture obtains is put into 1000L bacteria vats, carries out fermented and cultured 48 hours；
5) each pure culture for obtaining step 4) fermented and cultured centrifuges；
6) each pure culture that centrifugation obtains in step 5) is mixed, obtains Mixed Microbes；
7) Mixed Microbes are mixed with bentonite, kaolin, carbonic acid calcium uptake, dries, crushes, grinding turns into Mixed Microbes powder
Body；
8) polyethylene, azo dibutyronitrile, EVA, tin mercaptides and 16 carbon alcohol are added into successively in reactor, in 80 DEG C of temperature
Under degree, stir 5 hours, cool down blowing, obtain mixed material；
9) mixed material for obtaining step 8) is put into granulating extruder, extruding pelletization, obtains granular polyethylene
Tiny ecosystem agricultural plastic matrix.
A kind of seedling-cultivation plate, it is made up of polyethylene Tiny ecosystem agricultural plastic matrix through extrusion forming.
The preparation method of seedling-cultivation plate, comprises the following steps：
1) polyethylene Tiny ecosystem agricultural plastic matrix is put into calender, in the case where temperature is 200 DEG C, carries out tabletting, obtain
To sheet material；
2) sheet material obtained in step 1) is put into seedling disc shaping machine, in the case where temperature is 130 DEG C, extrusion forming, obtained
To seedling-cultivation plate.
Embodiment 2
A kind of polyethylene Tiny ecosystem agricultural plastic matrix, include the component of following parts by weight：30 parts of polyethylene, 12 parts of swellings
Soil, 15 parts of kaolin, 20 parts of calcium carbonate, 0.5 part of isopropyl benzene hydroperoxide, 0.5 part of PEO, 0.8 part of tin mercaptides, 1 part of 16 carbon alcohol
With 1 part of mixing probiotics.
Wherein mixing probiotics includes the strain of following mass percent：56% clostridium, 28% bar-shaped gemma
Bacillus and 16% lactic acid bacillus.The clostridium includes the strain of following mass percent：20% lichens gemma
Bacillus, 38% bacillus subtilis and 42% bacillus amyloliquefaciens.The bar-shaped bacillus includes following mass percent
Strain：33% bacillus laterosporus and 67% bacillus thuringiensis.The lactic acid bacillus includes following quality percentage
Several strains：12% butyric acid bacillus and 88% acetic acid bacillus.The particle diameter of bentonite, kaolin and calcium carbonate is to receive
Meter level.
The preparation method of polyethylene Tiny ecosystem agricultural plastic matrix, comprises the following steps：
1) each pure culture is distinguished into oscillation and fermentation cultivation；Using 500ml triangular flasks, built-in 50ml fermentation mediums, 50
DEG C, under 200r/min, shaken cultivation 30h, wherein, each pure culture is bacillus licheniformis, bacillus subtilis, solution starch respectively
Bacillus, bacillus laterosporus, bacillus thuringiensis, butyric acid bacillus or acetic acid bacillus；
2) each pure culture after fermented culture in step 1) is centrifuged；
3) each pure culture that centrifugation obtains in step 2) is put into 100L seeding tanks, culture 10 is enlarged at 45 DEG C
Hour；
4) each pure culture that step 3) expansion culture obtains is put into 1000L bacteria vats, carries out fermented and cultured 32 hours；
5) each pure culture for obtaining step 4) fermented and cultured centrifuges；
6) each pure culture that centrifugation obtains in step 5) is mixed, obtains Mixed Microbes；
7) Mixed Microbes are mixed with bentonite, kaolin, carbonic acid calcium uptake, dries, crushes, grinding turns into Mixed Microbes powder
Body；
8) polyethylene, isopropyl benzene hydroperoxide, phase solvent, stabilizer and lubricant are added into successively in reactor,
At a temperature of 100 DEG C, stir 3 hours, cool down blowing, obtain mixed material；
9) mixed material for obtaining step 8) is put into granulating extruder, extruding pelletization, obtains granular polyethylene
Tiny ecosystem agricultural plastic matrix.
A kind of seedling-cultivation plate, the seedling-cultivation plate are made up of polyethylene Tiny ecosystem agricultural plastic matrix through extrusion forming.
The preparation method of seedling-cultivation plate, comprises the following steps：
1) polyethylene Tiny ecosystem agricultural plastic matrix is put into calender, in the case where temperature is 200 DEG C, carries out tabletting, obtain
To sheet material；
2) sheet material obtained in step 1) is put into seedling disc shaping machine, in the case where temperature is 150 DEG C, extrusion forming, obtained
To seedling-cultivation plate.
Embodiment 3
A kind of polyethylene Tiny ecosystem agricultural plastic matrix, include the component of following parts by weight：30 parts of polyethylene, 15 parts of swellings
Soil, 20 parts of kaolin, 30 parts of calcium carbonate, 1 part of azo dibutyronitrile, 0.1-5 parts EVA, 1 part of tin mercaptides, 2 part of 16 carbon alcohol and 2 parts
Mix probiotics.
Wherein mixing probiotics includes the strain of following mass percent：58% clostridium, 28% bar-shaped gemma
Bacillus and 14% lactic acid bacillus.The clostridium includes the strain of following mass percent：22% lichens gemma
Bacillus, 40% bacillus subtilis and 38% bacillus amyloliquefaciens.The bar-shaped bacillus includes following mass percent
Strain：35% bacillus laterosporus and 65% bacillus thuringiensis.The lactic acid bacillus includes following quality percentage
Several strains：15% butyric acid bacillus and 85% acetic acid bacillus.The particle diameter of bentonite, kaolin and calcium carbonate is to receive
Meter level.
The method one of the preparation method of polyethylene Tiny ecosystem agricultural plastic matrix and the preparation method of seedling-cultivation plate and embodiment 1
Sample.
Embodiment 4
A kind of polyethylene Tiny ecosystem agricultural plastic matrix, include the component of following parts by weight：40 parts of polyethylene, 16 parts of swellings
Soil, 22 parts of kaolin, 40 parts of calcium carbonate, 0.6 part of azo dibutyronitrile, 1 part of EVA, 1.5 parts of tin mercaptides, 1.5 part of 16 carbon alcohol and 3
Part mixing probiotics.
Wherein mixing probiotics includes the strain of following mass percent：60% clostridium, 25% bar-shaped gemma
Bacillus and 15% lactic acid bacillus.The clostridium includes the strain of following mass percent：25% lichens gemma
Bacillus, 35% bacillus subtilis and 40% bacillus amyloliquefaciens.The bar-shaped bacillus includes following mass percent
Strain:38% bacillus laterosporus and 62% bacillus thuringiensis.The lactic acid bacillus includes following quality percentage
Several strains:18% butyric acid bacillus and 82% acetic acid bacillus.The particle diameter of bentonite, kaolin and calcium carbonate is to receive
Meter level.
The method one of the preparation method of polyethylene Tiny ecosystem agricultural plastic matrix and the preparation method of seedling-cultivation plate and embodiment 2
Sample.
Embodiment 5
A kind of polyethylene Tiny ecosystem agricultural plastic matrix, include the component of following parts by weight：50 parts of polyethylene, 20 parts of swellings
Soil, 30 parts of kaolin, 60 parts of calcium carbonate, 1 part of isopropyl benzene hydroperoxide, 5 parts of EVA or PEO, 2 parts of tin mercaptides, 5 part of 16 carbon alcohol
With 5 parts of mixing probiotics.
Wherein mixing probiotics includes the strain of following mass percent:60% clostridium, 30% bar-shaped gemma
Bacillus and 10% lactic acid bacillus.The clostridium includes the strain of following mass percent：20% lichens gemma
Bacillus, 42% bacillus subtilis and 38% bacillus amyloliquefaciens.The bar-shaped bacillus includes following mass percent
Strain:40% bacillus laterosporus and 60% bacillus thuringiensis.The lactic acid bacillus includes following quality percentage
Several strains:20% butyric acid bacillus and 80% acetic acid bacillus.The particle diameter of bentonite, kaolin and calcium carbonate is to receive
Meter level.
The method one of the preparation method of polyethylene Tiny ecosystem agricultural plastic matrix and the preparation method of seedling-cultivation plate and embodiment 2
Sample.
Using the conventional viable plate counts detection embodiment 1-5 polyethylene Tiny ecosystem agricultural plastic matrixs prepared and
Seedling-cultivation plate into number of viable, wherein the number of viable of the Tiny ecosystem agricultural plastic matrix for preparing and seedling-cultivation plate is high, Tiny ecosystem agriculture
Industry masterbatch into number of viable be 500-1000 hundred million/gram；Seedling-cultivation plate into number of viable be 1,000,000/gram more than.
Embodiment 6
This is that the seedling-cultivation plate prepared using the present invention carries out nursery effect research.
The nursery effect of the seedling-cultivation plate prepared for the exploratory development present invention, foundation is provided to put into practice production application.With routine
Blanket disk is control group, has carried out nursery effect contrast test.Experiment to rice shoot plant height, leaf age, radical, above and below ground dry weight,
Fresh weight etc. is investigated.
1st, test material
Seedling-cultivation plate prepared by the present invention
Conventional blanket disk (market purchase)
2nd, test site
Frequently get profit farm scientific and technological park (Heilongjiang Province Tongjiang City)
3rd, experimental design
Experiment is located in a greenhouse, and test specimen amount is 300-500 disks.Rice varieties select imperial round-grained rice 31 and imperial round-grained rice 46,
Conventional young plants management is carried out according to Rice Production technical regulation standard, the nursery effect and routine of seedling-cultivation plate prepared by the present invention
The nursery effect of blanket disk is contrasted.
4th, investigate and analyse
Test process is in balance on March 20, sowing on April 7, it has been observed that the seedling-cultivation plate prepared using the present invention is cultivated
Rice shoot seedling-growing time be April 12, and use the rice shoot seedling-growing time that conventional blanket disk is cultivated as April 14.Before transplanting, 5
The nursery effect of two kinds of seedling-cultivation plates of the investigation of the moon 10, the seedling quality of investigation are done including plant height, radical, leaf age, ground, underground
Weight, fresh weight etc..Averaging of income result is as shown in table 1 below.
The seedling quality of table 1 is investigated
From table 1 it follows that the rice shoot plant height cultivated using the seedling-cultivation plate of the present invention is than seedling that conventional blanket disk is cultivated
Height of seedling 1.5cm, more 0.2 leaf of seedling leaf, radical is more 2, the wide more 0.2mm of stem foot, up to a hundred plants of the ground more 5.2g of fresh weight, underground hundred
The more 1.6g of strain fresh weight.In summary, the plant height of rice shoot can be increased using the seedling-cultivation plate nursery of the present invention, using educating for the present invention
The indices for the rice shoot that seedling disk is cultivated are above the rice shoot cultivated using conventional blanket disk.Fig. 1 is prepared using the present invention
The seedling growth growing way photo of 12 days that seedling-cultivation plate is cultivated, Fig. 2 are the seedling growth length of 12 days cultivated using conventional blanket disk
Gesture photo.Comparison diagram 1 and Fig. 2, it can be seen that the rice shoot growing way that the seedling-cultivation plate prepared using the present invention is cultivated is fine, grows ratio
Richer, blade is glossy and green, and blade is without hole, and no pest infestation, average plant height is 5.5cm；And conventional blanket disk is cultivated
Rice shoot growing way it is poor, grow than sparse, blade is partially yellow, blade hole once in a while, and average plant height is 4cm.
Fig. 3 is the root growth feelings of the rice shoot for the identical plant height that blanket disk that the seedling-cultivation plate of the present invention is cultivated and conventional is cultivated
The photo of condition, the wherein left side are the root growth situation for the rice shoot that the seedling-cultivation plate prepared using the present invention is cultivated, and the right is to use
The root growth situation for the rice shoot that conventional blanket disk is cultivated.Understood by contrast, what the seedling-cultivation plate prepared using the present invention was cultivated
The radical of rice shoot is significantly more than the radical for the rice shoot cultivated using conventional blanket disk.
Fig. 4 is the seedling growth growing state of 15 days that blanket disk cultivated using the seedling-cultivation plate of the present invention and conventional is cultivated
Photo, the wherein left side is the growing state for the rice shoot cultivated using the seedling-cultivation plate for preparing of the present invention, and the right is to use conventional blanket
The growing state for the rice shoot that formula disk is cultivated.Understood by contrast, the growing way for the rice shoot that the seedling-cultivation plate prepared using the present invention is cultivated
Very well, rice shoot is relatively more strong, and root system is more flourishing；And the rice shoot for using conventional blanket disk to cultivate grows relatively fine, root system is owed
Reach, root have also appeared yellow leaf.
Fig. 5 is using the photo of the root growth situation before the shoot transplanting equipment of the seedling-cultivation plate of present invention cultivation.Can be with by Fig. 5
To find out, the root system for the rice shoot cultivated using the seedling-cultivation plate of the present invention is very flourishing, and packing effect is good, after being advantageous to transplanting, rice shoot
Fast-growth.
Claims (10)
1. a kind of polyethylene Tiny ecosystem agricultural plastic matrix, it is characterised in that include the component of following parts by weight：20-50 parts are gathered
Ethene, 10-20 parts bentonite, 10-30 parts kaolin, 10-60 parts calcium carbonate, 0.1-1 parts catalyst, 0.1-5 parts compatilizer,
0.5-2 parts stabilizer and 0.5-5 parts lubricant and 0.5-5 parts mixing probiotics.
2. polyethylene Tiny ecosystem agricultural plastic matrix according to claim 1, it is characterised in that the prebiotic bacterium bag of mixing
Include the strain of following mass percent：The bar-shaped bacillus of 55-60% clostridium, 25-30% and 10-16% lactic acid buds
Spore bacillus.
3. polyethylene Tiny ecosystem agricultural plastic matrix according to claim 2, it is characterised in that the clostridium
Include the strain of following mass percent：18-25% bacillus licheniformis, 35-45% bacillus subtilises and 37-42% Xie Dian
Afnyloliquefaciens.
4. polyethylene Tiny ecosystem agricultural plastic matrix according to claim 2, it is characterised in that the bar-shaped bacillus
Include the strain of following mass percent：30-40% bacillus laterosporuses and 60-70% bacillus thuringiensis.
5. polyethylene Tiny ecosystem agricultural plastic matrix according to claim 2, it is characterised in that the lactic acid bacillus
Include the strain of following mass percent：10-20% butyric acid bacillus and 80-90% acetic acid bacillus.
6. polyethylene Tiny ecosystem agricultural plastic matrix according to claim 1, it is characterised in that the catalyst is azo
Dibutyronitrile or isopropyl benzene hydroperoxide；The compatilizer is EVA or PEO；The stabilizer is organic tin；The lubricant is
Higher alcohols.
7. polyethylene Tiny ecosystem agricultural plastic matrix according to claim 1, it is characterised in that the bentonite, kaolinite
The particle diameter of soil and calcium carbonate is nanoscale.
8. the preparation method of the polyethylene Tiny ecosystem agricultural plastic matrix any one of claim 1-7, it is characterised in that
Comprise the following steps：
1) each pure culture is distinguished into oscillation and fermentation cultivation；
2) each pure culture after fermented culture in step 1) is centrifuged；
3) each pure culture that centrifugation obtains in step 2) is put into seeding tank, is enlarged culture；
4) each pure culture that step 3) expansion culture obtains is put into bacteria vat and carries out fermented and cultured；
5) each pure culture for obtaining step 4) fermented and cultured centrifuges；
6) each pure culture that centrifugation obtains in step 5) is mixed, obtains Mixed Microbes；
7) Mixed Microbes are mixed with bentonite, kaolin, carbonic acid calcium uptake, dries, crushes, grinding turns into Mixed Microbes powder；
8) polyethylene, catalyst, phase solvent, stabilizer and lubricant are added into successively in reactor, in 80-120 DEG C of temperature
Under, 1-5 hours are stirred, blowing is cooled down, obtains mixed material；
9) mixed material for obtaining step 8) is put into granulating extruder, extruding pelletization, obtains the micro- life of granular polyethylene
State agricultural plastic matrix.
9. a kind of seedling-cultivation plate, it is characterised in that the seedling-cultivation plate is as the polyethylene Tiny ecosystem any one of claim 1-7
Agriculture plastic matrix is made through extrusion forming.
10. the preparation method of the seedling-cultivation plate described in claim 9, it is characterised in that comprise the following steps：
1) the polyethylene Tiny ecosystem agricultural plastic matrix any one of claim 1-7 is put into calender and pressed
Piece, obtain sheet material；
2) sheet material obtained in step 1) is put into seedling disc shaping machine, extrusion forming, obtains seedling-cultivation plate.
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| CN1052123A (en) * | 1989-10-05 | 1991-06-12 | 生物信息公司 | Biologically degradable combination film and preparation method thereof |
| CN102369105A (en) * | 2008-09-24 | 2012-03-07 | 莱斯勒斯公司 | Introduction of heat-and/or pressure-resistant organisms into materials |
| CN102766308A (en) * | 2012-08-15 | 2012-11-07 | 肖伯文 | Composite, seedling bed made of composite and preparation method of seedling bed |
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2017
- 2017-08-07 CN CN201710667568.2A patent/CN107474356B/en active Active
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|---|---|---|---|---|
| CN1052123A (en) * | 1989-10-05 | 1991-06-12 | 生物信息公司 | Biologically degradable combination film and preparation method thereof |
| CN102369105A (en) * | 2008-09-24 | 2012-03-07 | 莱斯勒斯公司 | Introduction of heat-and/or pressure-resistant organisms into materials |
| CN102766308A (en) * | 2012-08-15 | 2012-11-07 | 肖伯文 | Composite, seedling bed made of composite and preparation method of seedling bed |
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| 云南省科学技术情报研究院: "益生菌用于有机农业种植的方法", 《农村实用技术》 * |
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