CN107474356B - Polyethylene micro-ecological agricultural plastic master batch and seedling raising tray made of same - Google Patents
Polyethylene micro-ecological agricultural plastic master batch and seedling raising tray made of same Download PDFInfo
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- CN107474356B CN107474356B CN201710667568.2A CN201710667568A CN107474356B CN 107474356 B CN107474356 B CN 107474356B CN 201710667568 A CN201710667568 A CN 201710667568A CN 107474356 B CN107474356 B CN 107474356B
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- -1 Polyethylene Polymers 0.000 title claims abstract description 49
- 239000004698 Polyethylene Substances 0.000 title claims abstract description 48
- 229920000573 polyethylene Polymers 0.000 title claims abstract description 48
- 239000004594 Masterbatch (MB) Substances 0.000 title claims abstract description 44
- 229920003023 plastic Polymers 0.000 title claims abstract description 42
- 239000004033 plastic Substances 0.000 title claims abstract description 42
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 44
- 238000000034 method Methods 0.000 claims abstract description 25
- 239000005995 Aluminium silicate Substances 0.000 claims abstract description 22
- 235000012211 aluminium silicate Nutrition 0.000 claims abstract description 22
- 239000000440 bentonite Substances 0.000 claims abstract description 22
- 229910000278 bentonite Inorganic materials 0.000 claims abstract description 22
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims abstract description 22
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 22
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000006041 probiotic Substances 0.000 claims abstract description 22
- 235000018291 probiotics Nutrition 0.000 claims abstract description 22
- 239000000314 lubricant Substances 0.000 claims abstract description 8
- 239000003381 stabilizer Substances 0.000 claims abstract description 8
- 239000003054 catalyst Substances 0.000 claims abstract description 7
- 238000000855 fermentation Methods 0.000 claims description 63
- 230000004151 fermentation Effects 0.000 claims description 63
- 241000894006 Bacteria Species 0.000 claims description 17
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 15
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 14
- 244000063299 Bacillus subtilis Species 0.000 claims description 14
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 14
- 241000193403 Clostridium Species 0.000 claims description 14
- 241000186660 Lactobacillus Species 0.000 claims description 14
- 229940039696 lactobacillus Drugs 0.000 claims description 14
- 238000002360 preparation method Methods 0.000 claims description 13
- 241000194108 Bacillus licheniformis Species 0.000 claims description 12
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 12
- 241000193417 Brevibacillus laterosporus Species 0.000 claims description 12
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 12
- 238000010899 nucleation Methods 0.000 claims description 12
- 241000186216 Corynebacterium Species 0.000 claims description 11
- 230000003321 amplification Effects 0.000 claims description 11
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 10
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 8
- 238000001125 extrusion Methods 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 239000002245 particle Substances 0.000 claims description 8
- FRIBMENBGGCKPD-UHFFFAOYSA-N 3-(2,3-dimethoxyphenyl)prop-2-enal Chemical compound COC1=CC=CC(C=CC=O)=C1OC FRIBMENBGGCKPD-UHFFFAOYSA-N 0.000 claims description 5
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 238000007599 discharging Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 238000005469 granulation Methods 0.000 claims description 4
- 230000003179 granulation Effects 0.000 claims description 4
- 238000000227 grinding Methods 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- YCIGYTFKOXGYTA-UHFFFAOYSA-N 4-(3-cyanopropyldiazenyl)butanenitrile Chemical group N#CCCCN=NCCCC#N YCIGYTFKOXGYTA-UHFFFAOYSA-N 0.000 claims description 2
- 230000012010 growth Effects 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 12
- 238000004321 preservation Methods 0.000 abstract description 10
- 230000009286 beneficial effect Effects 0.000 abstract description 7
- 239000000575 pesticide Substances 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 5
- 230000035784 germination Effects 0.000 abstract description 4
- 238000003973 irrigation Methods 0.000 abstract description 3
- 230000002262 irrigation Effects 0.000 abstract description 3
- 238000012856 packing Methods 0.000 abstract description 3
- 208000035240 Disease Resistance Diseases 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 22
- 239000002609 medium Substances 0.000 description 16
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 14
- 240000008042 Zea mays Species 0.000 description 14
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 14
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 14
- 235000005822 corn Nutrition 0.000 description 14
- 238000012258 culturing Methods 0.000 description 14
- 238000004659 sterilization and disinfection Methods 0.000 description 14
- 238000004519 manufacturing process Methods 0.000 description 9
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 8
- 235000021186 dishes Nutrition 0.000 description 8
- 244000046052 Phaseolus vulgaris Species 0.000 description 7
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 7
- 235000015278 beef Nutrition 0.000 description 7
- 235000013312 flour Nutrition 0.000 description 7
- 239000011521 glass Substances 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 7
- BXWNKGSJHAJOGX-UHFFFAOYSA-N hexadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCO BXWNKGSJHAJOGX-UHFFFAOYSA-N 0.000 description 7
- 235000015110 jellies Nutrition 0.000 description 7
- 239000008274 jelly Substances 0.000 description 7
- 239000006916 nutrient agar Substances 0.000 description 7
- 230000010355 oscillation Effects 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 238000000926 separation method Methods 0.000 description 7
- 239000011780 sodium chloride Substances 0.000 description 7
- 230000001954 sterilising effect Effects 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 230000002786 root growth Effects 0.000 description 6
- 244000283763 Acetobacter aceti Species 0.000 description 4
- 235000007847 Acetobacter aceti Nutrition 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000589220 Acetobacter Species 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 238000002054 transplantation Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013400 design of experiment Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L23/00—Compositions of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Compositions of derivatives of such polymers
- C08L23/02—Compositions of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Compositions of derivatives of such polymers not modified by chemical after-treatment
- C08L23/04—Homopolymers or copolymers of ethene
- C08L23/06—Polyethene
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0293—Seed or shoot receptacles
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- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/20—Compounding polymers with additives, e.g. colouring
- C08J3/22—Compounding polymers with additives, e.g. colouring using masterbatch techniques
- C08J3/226—Compounding polymers with additives, e.g. colouring using masterbatch techniques using a polymer as a carrier
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C08J2327/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a halogen; Derivatives of such polymers
- C08J2327/02—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a halogen; Derivatives of such polymers not modified by chemical after-treatment
- C08J2327/04—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a halogen; Derivatives of such polymers not modified by chemical after-treatment containing chlorine atoms
- C08J2327/06—Homopolymers or copolymers of vinyl chloride
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2423/00—Characterised by the use of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Derivatives of such polymers
- C08J2423/02—Characterised by the use of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Derivatives of such polymers not modified by chemical after treatment
- C08J2423/04—Homopolymers or copolymers of ethene
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- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
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- C08K3/18—Oxygen-containing compounds, e.g. metal carbonyls
- C08K3/24—Acids; Salts thereof
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Abstract
The invention discloses a polyethylene micro-ecological agricultural plastic master batch and a seedling raising tray prepared from the master batch, wherein the master batch comprises the following components in parts by weight: 20-50 parts of polyethylene, 10-20 parts of bentonite, 10-30 parts of kaolin, 10-60 parts of calcium carbonate, 0.1-1 part of catalyst, 0.1-5 parts of compatilizer, 0.5-2 parts of stabilizer, 0.5-5 parts of lubricant and 0.5-5 parts of mixed probiotics. The seedling raising tray prepared from the polyethylene micro-ecological agricultural plastic master batch is well formed, and the added nano-scale bentonite, kaolin and calcium carbonate have the effects of moisture preservation and heat preservation, are beneficial to seedling germination and reduce the labor intensity of irrigation; the probiotics beneficial to the growth of the seedlings are added, so that the seedlings have certain disease resistance, the roots of the seedlings have more roots, the packing effect is good, the stems are thick and strong, the emergence rate and the survival rate are high, the fertilizing amount and the pesticide amount in the seedling raising process are reduced, and the labor force is greatly reduced.
Description
Technical Field
The invention relates to the field of plastic master batches for agriculture, in particular to a polyethylene microecological agricultural plastic master batch containing probiotics and a seedling raising tray prepared from the same.
Background
The existing rice seedling raising technology mainly adopts a seedling raising tray to raise seedlings, and the seedling tray is transplanted into a field after the seedlings grow to a certain degree. The seedling raising is an important process, and the seedlings can grow well in the seedling raising process, so that the later growth of the seedlings is facilitated. The seedling raising tray used in the seedling raising process has certain influence on the sprouting rate and the growth condition of the seedlings, so that the growth vigor of the seedlings in the seedling raising process can be improved and the sprouting rate of the seedlings can be improved by improving the condition of the seedling raising tray.
At present, the seedling raising tray commonly used in agriculture is not environment-friendly and difficult to recycle in the using process, and easily causes environmental pollution. In addition, the seedling raising tray has short service life, can be used only once basically, has reduced quality when being reused for the second time, and cannot meet the requirements of seedling raising. The conventional seedling raising tray is only used for temporarily raising seedlings and cannot provide other nutrients for the growth process of the seedlings, so that the seedling raising process also needs to regularly fertilize and spray insecticide and pesticide, the labor force is increased, and the emergence rate, the survival rate, the emergence quality and the like of the seedlings are also influenced.
Therefore, a seedling raising tray which is easy to recycle, environment-friendly, free from secondary pollution, capable of improving the germination rate and survival rate of seedlings and reducing the amount of fertilizer and pesticide in the seedling raising process is needed.
Disclosure of Invention
The invention adds a plurality of probiotics into polyethylene to prepare plastic master batch containing the probiotics, and then the plastic master batch is processed into a seedling raising tray.
The invention aims to solve the technical problem that the plastic master batch containing probiotics is provided aiming at the defects of the prior art, is a recyclable plastic product and does not cause secondary pollution, and the seedling raising tray made of the plastic master batch can improve the sprouting rate and the survival rate of seedlings, reduce the fertilizing amount and the pesticide amount in the seedling raising process, ensure the healthy growth of the seedling raising seedlings and reduce the heavy labor force.
In order to solve the technical problems, the invention adopts the technical scheme that: a polyethylene micro-ecological agricultural plastic master batch comprises the following components in parts by weight: 20-50 parts of polyethylene, 10-20 parts of bentonite, 10-30 parts of kaolin, 10-60 parts of calcium carbonate, 0.1-1 part of catalyst, 0.1-5 parts of compatilizer, 0.5-2 parts of stabilizer, 0.5-5 parts of lubricant and 0.5-5 parts of mixed probiotics.
Preferably, the mixed probiotics comprise the following strains in percentage by mass: 55-60% of clostridium, 25-30% of corynebacterium and 10-16% of lactobacillus.
Preferably, the clostridium comprises the following strains in percentage by mass: 18-25% of bacillus licheniformis, 35-45% of bacillus subtilis and 37-42% of bacillus amyloliquefaciens.
The bacillus subtilis and the bacillus amyloliquefaciens can be well known, such as bacillus amyloliquefaciens with the preservation number of CGMCC NO.13643 and bacillus subtilis with the preservation number of CGMCC NO.13642 in China center for culture collection of microorganisms.
The bacillus comprises the following strains in percentage by mass: 30-40% of Bacillus laterosporus and 60-70% of Bacillus thuringiensis.
Preferably, the lactobacillus comprises the following strains in percentage by mass: 10-20% of butyric acid bacillus and 80-90% of acetic acid bacillus.
The strain of the present invention may be known, for example, Bacillus licheniformis provided by Guangdong Heiyou Ribo Biotech Co., Ltd, Bacillus laterosporus provided by Henan Yikang Bio-Tech Co., Ltd, Bacillus thuringiensis provided by Guangzhou Ba agricultural Biotech Co., Ltd, Bacillus butyricum and Bacillus acetate provided by Shandong Scohio bioengineering GmbH.
Preferably, the catalyst is azobisbutyronitrile or cumene hydroperoxide; the compatibilizer is EVA or PEO; the stabilizer is an organotin; the lubricant is a higher alcohol.
The organic tin is tin mercaptide, and the higher alcohol is hexadecanol.
Preferably, the particle sizes of the bentonite, the kaolin and the calcium carbonate are all nano-scale. The bentonite, kaolin and calcium carbonate with nano-grade particle size are added, and the materials have the effects of moisture preservation, water absorption and heat preservation, thereby being beneficial to improving the germination rate of seedlings and reducing the labor intensity of irrigation.
The invention provides a preparation method of a polyethylene micro-ecological agricultural plastic master batch, which comprises the following steps:
1) respectively carrying out shaking fermentation culture on each pure strain;
2) centrifugally separating each pure strain subjected to fermentation culture in the step 1);
3) putting each pure strain obtained by centrifugation in the step 2) into a seeding tank for amplification culture;
4) putting each pure strain obtained by the enlarged culture in the step 3) into a fermentation pot for fermentation culture;
5) centrifugally separating each pure strain obtained by fermentation culture in the step 4);
6) mixing all pure strains obtained by centrifugation in the step 5) to obtain mixed strains;
7) absorbing and mixing the mixed bacteria with bentonite, kaolin and calcium carbonate, drying, crushing and grinding into mixed bacteria powder;
8) sequentially adding polyethylene, a catalyst, a phase solvent, a stabilizer and a lubricant into a reaction kettle, stirring for 1-5 hours at the temperature of 80-120 ℃, cooling and discharging to obtain a mixed material;
9) putting the mixed material obtained in the step 8) into a granulation extruder, and extruding and granulating to obtain the granular polyethylene micro-ecological agricultural plastic master batch.
Specifically, the cultivation of various bacteria is as follows:
1. and (3) culturing the bacillus licheniformis:
the strain was retrieved from Guangdong Whitman Biotech Limited for expanded culture.
Culture process equipment and apparatus: one high-temperature disinfection pot is used; a constant temperature water tower is provided; one set/sleeve of tubular centrifuge; one set/set of ultra-clean sterilization cabinet; one set/set of THZ-92A desk type constant temperature oscillator (oscillation amplitude 25 mm); a microscope; petri dishes, glass instruments batch.
The cultivation and production process comprises: slant culture medium: beef extract: 0.2 to 1.0 percent; protein jelly: 0.5-1.0%; nutrient agar: 0.5-1.0%; NaCl: 0.1-0.5% and the balance of water.
Fermentation medium: bean pulp: 1 to 5 percent; corn flour: 1 to 3 percent; corn steep liquor: 0.2 to 1.0 percent; KHPO4: 0.1 to 0.3 percent; the balance being water.
Fermentation culture: adopting 500ml triangular flask, charging 50ml fermentation medium, shaking culturing at 30-80 deg.C 200r/min for 10-40 h.
Separating the strain after fermentation culture by a centrifugal machine, putting the strain into a 100L seeding tank, carrying out amplification culture for 8-12 hours at 40-80 ℃, then putting the strain into a 1000L fermentation pot, carrying out fermentation for 24-48 hours, and after the strain is qualified, putting the strain into the centrifugal machine for separation to obtain the bacillus licheniformis.
2. Culturing the bacillus subtilis:
the bacillus subtilis with the preservation number of CGMCC NO.13642 is retrieved from the preservation center for expanded culture.
Culture process equipment and apparatus: one high-temperature disinfection pot is used; a constant temperature water tower is provided; one set/sleeve of tubular centrifuge; one set/set of ultra-clean sterilization cabinet; one set/set of THZ-92A desk type constant temperature oscillator (oscillation amplitude 25 mm); a microscope; petri dishes, glass instruments batch.
The cultivation and production process comprises: slant culture medium: beef extract: 0.2 to 1.0 percent; protein jelly: 0.5-1.0%; nutrient agar: 0.5-1.0%; NaCl: 0.1-0.5% and the balance of water.
Fermentation medium: bean pulp: 1 to 5 percent; corn flour: 1 to 3 percent; corn steep liquor: 0.2 to 1.0 percent; KHPO4: 0.1 to 0.3 percent; the balance being water.
Fermentation culture: adopting 500ml triangular flask, charging 50ml fermentation medium, shaking culturing at 30-80 deg.C 200r/min for 10-40 h.
Separating the strain after fermentation culture by a centrifugal machine, feeding the strain into a 100L seeding tank, carrying out amplification culture for 8-12 hours at the temperature of 40-80 ℃, then feeding the strain into a 1000L fermentation pot for fermentation for 24-48 hours, and after the strain is qualified, feeding the strain into the centrifugal machine for separation to obtain the bacillus subtilis.
3. Culturing the bacillus amyloliquefaciens:
and (3) taking the bacillus amyloliquefaciens with the preservation number of CGMCC NO.13643 from the preservation center for expanded culture.
Culture process equipment and apparatus: one high-temperature disinfection pot is used; a constant temperature water tower is provided; one set/sleeve of tubular centrifuge; one set/set of ultra-clean sterilization cabinet; one set/set of THZ-92A desk type constant temperature oscillator (oscillation amplitude 25 mm); a microscope; petri dishes, glass instruments batch.
The cultivation and production process comprises: slant culture medium: beef extract: 0.2 to 1.0 percent; protein jelly: 0.5-1.0%; nutrient agar: 0.5-1.0%; NaCl: 0.1-0.5% and the balance of water.
Fermentation medium: bean pulp: 1 to 5 percent; corn flour: 1 to 3 percent; corn steep liquor: 0.2 to 1.0 percent; KHPO4: 0.1 to 0.3 percent; the balance being water.
Fermentation culture: adopting 500ml triangular flask, charging 50ml fermentation medium, shaking culturing at 30-80 deg.C 200r/min for 10-40 h.
Separating the strain after fermentation culture by a centrifugal machine, feeding the strain into a 100L seeding tank, carrying out amplification culture for 8-12 hours at the temperature of 40-80 ℃, then feeding the strain into a 1000L fermentation pot for fermentation for 24-48 hours, and after the strain is qualified, feeding the strain into the centrifugal machine for separation to obtain the bacillus amyloliquefaciens.
4. Culturing of bacillus laterosporus:
the strain retrieval and scale-up culture is provided by Henan Yikang biology Ltd.
Culture process equipment and apparatus: one high-temperature disinfection pot is used; a constant temperature water tower is provided; one set/sleeve of tubular centrifuge; one set/set of ultra-clean sterilization cabinet; one set/set of THZ-92A desk type constant temperature oscillator (oscillation amplitude 25 mm); a microscope; petri dishes, glass instruments batch.
The cultivation and production process comprises: slant culture medium: beef extract: 0.2 to 1.0 percent; protein jelly: 0.5-1.0%; nutrient agar: 0.5-1.0%; NaCl: 0.1-0.5% and the balance of water.
Fermentation medium: bean pulp: 1 to 5 percent; corn flour: 1 to 3 percent; corn steep liquor: 0.2 to 1.0 percent; KHPO4: 0.1 to 0.3 percent; the balance being water.
Fermentation culture: adopting 500ml triangular flask, charging 50ml fermentation medium, shaking culturing at 30-80 deg.C 200r/min for 10-40 h.
Separating the fermented strain by a centrifugal machine, feeding the strain into a 100L seeding tank, carrying out amplification culture for 8-12 hours at the temperature of 40-80 ℃, then feeding the strain into a 1000L fermentation pot for fermentation for 24-48 hours, and after the strain is qualified, feeding the strain into the centrifugal machine for separation to obtain the bacillus laterosporus.
5. Culturing bacillus thuringiensis:
the strain is retrieved from Guangzhou Banong Biotech Co and expanded.
Culture process equipment and apparatus: one high-temperature disinfection pot is used; a constant temperature water tower is provided; one set/sleeve of tubular centrifuge; one set/set of ultra-clean sterilization cabinet; one set/set of THZ-92A desk type constant temperature oscillator (oscillation amplitude 25 mm); a microscope; petri dishes, glass instruments batch.
The cultivation and production process comprises: slant culture medium: beef extract: 0.2 to 1.0 percent; protein jelly: 0.5-1.0%; nutrient agar: 0.5-1.0%; NaCl: 0.1-0.5% and the balance of water.
Fermentation medium: bean pulp: 1 to 5 percent; corn flour: 1 to 3 percent; corn steep liquor: 0.2 to 1.0 percent; KHPO4: 0.1 to 0.3 percent; the balance being water.
Fermentation culture: adopting 500ml triangular flask, charging 50ml fermentation medium, shaking culturing at 30-80 deg.C 200r/min for 10-40 h.
Separating the strain after fermentation culture by a centrifugal machine, feeding the strain into a 100L seeding tank, carrying out amplification culture for 8-12 hours at the temperature of 40-80 ℃, then feeding the strain into a 1000L fermentation pot for fermentation for 24-48 hours, and after the strain is qualified, feeding the strain into the centrifugal machine for separation to obtain the bacillus thuringiensis.
6. Culturing the butyric acid bacillus:
the strain is retrieved from Shandong Su Ke Han bioengineering GmbH and expanded.
Culture process equipment and apparatus: one high-temperature disinfection pot is used; a constant temperature water tower is provided; one set/sleeve of tubular centrifuge; one set/set of ultra-clean sterilization cabinet; one set/set of THZ-92A desk type constant temperature oscillator (oscillation amplitude 25 mm); a microscope; petri dishes, glass instruments batch.
The cultivation and production process comprises: slant culture medium: beef extract: 0.2 to 1.0 percent; protein jelly: 0.5-1.0%; nutrient agar: 0.5-1.0%; NaCl: 0.1-0.5% and the balance of water.
Fermentation medium: bean pulp: 1 to 5 percent; corn flour: 1 to 3 percent; corn steep liquor: 0.2 to 1.0 percent; KHPO4: 0.1 to 0.3 percent; the balance being water.
Fermentation culture: adopting 500ml triangular flask, charging 50ml fermentation medium, shaking culturing at 30-80 deg.C 200r/min for 10-40 h.
Separating the strain after fermentation culture by a centrifugal machine, feeding the strain into a 100L seeding tank, carrying out amplification culture for 8-12 hours at the temperature of 40-80 ℃, then feeding the strain into a 1000L fermentation pot for fermentation for 24-48 hours, and after the strain is qualified, feeding the strain into the centrifugal machine for separation to obtain the bacillus butyrate.
7. Culturing of bacillus aceticus:
the strain is retrieved from Shandong Su Ke Han bioengineering GmbH and expanded.
Culture process equipment and apparatus: one high-temperature disinfection pot is used; a constant temperature water tower is provided; one set/sleeve of tubular centrifuge; one set/set of ultra-clean sterilization cabinet; one set/set of THZ-92A desk type constant temperature oscillator (oscillation amplitude 25 mm); a microscope; petri dishes, glass instruments batch.
The cultivation and production process comprises: slant culture medium: beef extract: 0.2 to 1.0 percent; protein jelly: 0.5-1.0%; nutrient agar: 0.5-1.0%; NaCl: 0.1-0.5% and the balance of water.
Fermentation medium: bean pulp: 1 to 5 percent; corn flour: 1 to 3 percent; corn steep liquor: 0.2 to 1.0 percent; KHPO4: 0.1 to 0.3 percent; the balance being water.
Fermentation culture: adopting 500ml triangular flask, charging 50ml fermentation medium, shaking culturing at 30-80 deg.C 200r/min for 10-40 h.
Separating the strain after fermentation culture by a centrifugal machine, feeding the strain into a 100L seeding tank, carrying out amplification culture for 8-12 hours at the temperature of 40-80 ℃, then feeding the strain into a 1000L fermentation pot for fermentation for 24-48 hours, and after the strain is qualified, feeding the strain into the centrifugal machine for separation to obtain the bacillus butyrate.
The invention provides a seedling raising tray which is made of polyethylene micro-ecological agricultural plastic master batch through extrusion forming.
The invention provides a preparation method of a seedling raising tray, which comprises the following steps:
1) putting the polyethylene micro-ecological agricultural plastic master batch into a calender for tabletting, and controlling the temperature to be 150 ℃ and 210 ℃ to obtain a sheet;
2) placing the sheet obtained in the step 1) into a seedling raising tray forming machine, controlling the temperature to be 130-150 ℃, and performing extrusion forming to obtain the seedling raising tray.
The prepared polyethylene micro-ecological agricultural plastic master batch contains rich probiotics, can be directly used as fertilizer to be thrown into the roots of crops, can improve the hardening condition of soil, increase the fertility of the soil, supplement the fertilizer of the crops, enable the crops to thrive and grow, develop roots and stems, and improve the lodging resistance of the crops.
The invention has the beneficial effects that: the seedling raising tray prepared from the polyethylene micro-ecological agricultural plastic master batch is well formed, and the added nano-scale bentonite, kaolin and calcium carbonate have the effects of moisture preservation and heat preservation, are beneficial to seedling germination and reduce the labor intensity of irrigation; the probiotics beneficial to the growth of the seedlings are added, so that the seedlings have certain disease resistance, the roots of the seedlings have more roots, the packing effect is good, the stems are thick and strong, the emergence rate and the survival rate are high, the fertilizing amount and the pesticide amount in the seedling raising process are reduced, and the labor force is greatly reduced. The prepared micro-ecological agricultural plastic master batch and the seedling raising tray have high viable count, and the viable count of the micro-ecological agricultural master batch is 500 plus 1000 hundred million/g; the number of the grown live bacteria of the seedling raising tray is more than 100 ten thousand per gram.
Drawings
FIG. 1 is a photograph showing the growth of 12 days of seedlings cultivated by the seedling raising tray prepared according to the present invention;
FIG. 2 is a photograph showing the growth of seedlings grown for 12 days using a conventional blanket type tray;
FIG. 3 is a photograph showing the root growth of seedlings of the same plant height grown in the seedling raising tray of the present invention and in the conventional blanket tray;
FIG. 4 is a photograph showing 15-day growth of seedlings grown using the seedling raising tray of the present invention and conventional blanket trays;
FIG. 5 is a photograph showing the root growth of seedlings cultivated by the seedling tray of the present invention before transplantation.
Detailed Description
The technical solution of the present invention is further described in detail by the following examples.
Example 1
A polyethylene micro-ecological agricultural plastic master batch comprises the following components in parts by weight: 20 parts of polyethylene, 10 parts of bentonite, 10 parts of kaolin, 10 parts of calcium carbonate, 0.1 part of azodibutyrronitrile, 0.1 part of EVA, 0.5 part of tin mercaptide, 0.5 part of hexadecanol and 0.5 part of mixed probiotics. Wherein the mixed probiotics comprise the following strains in percentage by mass: 55% Clostridium, 30% Corynebacterium and 15% Lactobacillus.
Wherein, the clostridium comprises the following strains in percentage by mass: 18% bacillus licheniformis, 45% bacillus subtilis and 37% bacillus amyloliquefaciens. The corynebacterium comprises the following strains in percentage by mass: 30% of Bacillus laterosporus and 70% of Bacillus thuringiensis. The lactobacillus comprises the following strains in percentage by mass: 10% of B.butyricum and 90% of B.acetobacter. The particle sizes of the bentonite, the kaolin and the calcium carbonate are all nano-scale.
The preparation method of the polyethylene micro-ecological agricultural plastic master batch comprises the following steps:
1) respectively carrying out shaking fermentation culture on each pure strain; adopting a 500ml triangular flask, filling 50ml fermentation medium, and carrying out shake culture for 40h at 30 ℃ and 200r/min, wherein each pure bacterium is respectively bacillus licheniformis, bacillus subtilis, bacillus amyloliquefaciens, bacillus laterosporus, bacillus thuringiensis, bacillus butyricum or bacillus aceticus;
2) centrifugally separating each pure strain subjected to fermentation culture in the step 1);
3) putting each pure strain obtained by centrifugation in the step 2) into a 100L seeding tank, and carrying out amplification culture at 40 ℃ for 12 hours;
4) putting each pure strain obtained by the enlarged culture in the step 3) into a 1000L fermentation pot, and carrying out fermentation culture for 48 hours;
5) centrifugally separating each pure strain obtained by fermentation culture in the step 4);
6) mixing all pure strains obtained by centrifugation in the step 5) to obtain mixed strains;
7) absorbing and mixing the mixed bacteria with bentonite, kaolin and calcium carbonate, drying, crushing and grinding into mixed bacteria powder;
8) sequentially adding polyethylene, azodicarbonitrile, EVA, tin mercaptide and hexadecanol into a reaction kettle, stirring for 5 hours at the temperature of 80 ℃, cooling and discharging to obtain a mixed material;
9) putting the mixed material obtained in the step 8) into a granulation extruder, and extruding and granulating to obtain the granular polyethylene micro-ecological agricultural plastic master batch.
A seedling raising tray is made of polyethylene microecological agricultural plastic master batch through extrusion molding.
The preparation method of the seedling raising tray comprises the following steps:
1) putting the polyethylene micro-ecological agricultural plastic master batch into a calender, and tabletting at the temperature of 200 ℃ to obtain a sheet;
2) putting the sheet obtained in the step 1) into a seedling raising tray forming machine, and performing extrusion forming at the temperature of 130 ℃ to obtain the seedling raising tray.
Example 2
A polyethylene micro-ecological agricultural plastic master batch comprises the following components in parts by weight: 30 parts of polyethylene, 12 parts of bentonite, 15 parts of kaolin, 20 parts of calcium carbonate, 0.5 part of cumene hydroperoxide, 0.5 part of PEO, 0.8 part of tin mercaptide, 1 part of hexadecanol and 1 part of mixed probiotics.
Wherein the mixed probiotics comprise the following strains in percentage by mass: 56% Clostridium, 28% Corynebacterium and 16% Lactobacillus. The clostridium comprises the following strains in percentage by mass: 20% of Bacillus licheniformis, 38% of Bacillus subtilis and 42% of Bacillus amyloliquefaciens. The corynebacterium comprises the following strains in percentage by mass: 33% of bacillus laterosporus and 67% of bacillus thuringiensis. The lactobacillus comprises the following strains in percentage by mass: 12% of B.butyricum and 88% of B.acetobacter. The particle sizes of the bentonite, the kaolin and the calcium carbonate are all nano-scale.
The preparation method of the polyethylene micro-ecological agricultural plastic master batch comprises the following steps:
1) respectively carrying out shaking fermentation culture on each pure strain; adopting a 500ml triangular flask, filling 50ml fermentation medium, and carrying out shake culture for 30h at 50 ℃ and 200r/min, wherein each pure bacterium is respectively bacillus licheniformis, bacillus subtilis, bacillus amyloliquefaciens, bacillus laterosporus, bacillus thuringiensis, bacillus butyricum or bacillus aceticus;
2) centrifugally separating each pure strain subjected to fermentation culture in the step 1);
3) putting each pure strain obtained by centrifugation in the step 2) into a 100L seeding tank, and carrying out amplification culture for 10 hours at the temperature of 45 ℃;
4) putting each pure strain obtained by the enlarged culture in the step 3) into a 1000L fermentation pot, and carrying out fermentation culture for 32 hours;
5) centrifugally separating each pure strain obtained by fermentation culture in the step 4);
6) mixing all pure strains obtained by centrifugation in the step 5) to obtain mixed strains;
7) absorbing and mixing the mixed bacteria with bentonite, kaolin and calcium carbonate, drying, crushing and grinding into mixed bacteria powder;
8) sequentially adding polyethylene, cumene hydroperoxide, a phase solvent, a stabilizer and a lubricant into a reaction kettle, stirring for 3 hours at the temperature of 100 ℃, cooling and discharging to obtain a mixed material;
9) putting the mixed material obtained in the step 8) into a granulation extruder, and extruding and granulating to obtain the granular polyethylene micro-ecological agricultural plastic master batch.
A seedling raising tray is made of polyethylene micro-ecological agricultural plastic master batches through extrusion forming.
The preparation method of the seedling raising tray comprises the following steps:
1) putting the polyethylene micro-ecological agricultural plastic master batch into a calender, and tabletting at the temperature of 200 ℃ to obtain a sheet;
2) putting the sheet obtained in the step 1) into a seedling raising tray forming machine, and performing extrusion forming at the temperature of 150 ℃ to obtain the seedling raising tray.
Example 3
A polyethylene micro-ecological agricultural plastic master batch comprises the following components in parts by weight: 30 parts of polyethylene, 15 parts of bentonite, 20 parts of kaolin, 30 parts of calcium carbonate, 1 part of azodibutyrronitrile, 0.1-5 parts of EVA, 1 part of tin mercaptide, 2 parts of hexadecanol and 2 parts of mixed probiotics.
Wherein the mixed probiotics comprise the following strains in percentage by mass: 58% Clostridium, 28% Corynebacterium and 14% Lactobacillus. The clostridium comprises the following strains in percentage by mass: 22% Bacillus licheniformis, 40% Bacillus subtilis and 38% Bacillus amyloliquefaciens. The corynebacterium comprises the following strains in percentage by mass: 35% bacillus laterosporus and 65% bacillus thuringiensis. The lactobacillus comprises the following strains in percentage by mass: 15% of B.butyricum and 85% of B.acetobacter. The particle sizes of the bentonite, the kaolin and the calcium carbonate are all nano-scale.
The preparation method of the polyethylene micro-ecological agricultural plastic master batch and the preparation method of the seedling raising tray are the same as the method of the embodiment 1.
Example 4
A polyethylene micro-ecological agricultural plastic master batch comprises the following components in parts by weight: 40 parts of polyethylene, 16 parts of bentonite, 22 parts of kaolin, 40 parts of calcium carbonate, 0.6 part of azodibutyrronitrile, 1 part of EVA, 1.5 parts of tin mercaptide, 1.5 parts of hexadecanol and 3 parts of mixed probiotics.
Wherein the mixed probiotics comprise the following strains in percentage by mass: 60% Clostridium, 25% Corynebacterium and 15% Lactobacillus. The clostridium comprises the following strains in percentage by mass: 25% of Bacillus licheniformis, 35% of Bacillus subtilis and 40% of Bacillus amyloliquefaciens. The corynebacteria comprise the following strains, by mass, 38% of bacillus laterosporus and 62% of bacillus thuringiensis. The lactobacillus comprises the following strains of 18 percent of bacillus butyrate and 82 percent of bacillus acetate in percentage by mass. The particle sizes of the bentonite, the kaolin and the calcium carbonate are all nano-scale.
The preparation method of the polyethylene micro-ecological agricultural plastic master batch and the preparation method of the seedling raising tray are the same as the method of the embodiment 2.
Example 5
A polyethylene micro-ecological agricultural plastic master batch comprises the following components in parts by weight: 50 parts of polyethylene, 20 parts of bentonite, 30 parts of kaolin, 60 parts of calcium carbonate, 1 part of cumene hydroperoxide, 5 parts of EVA or PEO, 2 parts of tin mercaptide, 5 parts of hexadecanol and 5 parts of mixed probiotics.
Wherein the mixed probiotics comprise the following strains of 60 percent of clostridium, 30 percent of corynebacterium and 10 percent of lactobacillus in percentage by mass. The clostridium comprises the following strains in percentage by mass: 20% of Bacillus licheniformis, 42% of Bacillus subtilis and 38% of Bacillus amyloliquefaciens. The corynebacteria comprise the following strains, by mass, 40% of bacillus laterosporus and 60% of bacillus thuringiensis. The lactobacillus comprises the following strains of, by mass, 20% of bacillus butyricum and 80% of bacillus aceticus. The particle sizes of the bentonite, the kaolin and the calcium carbonate are all nano-scale.
The preparation method of the polyethylene micro-ecological agricultural plastic master batch and the preparation method of the seedling raising tray are the same as the method of the embodiment 2.
Detecting the number of finished viable bacteria of the polyethylene micro-ecological agricultural plastic master batch and the seedling raising tray prepared in the examples 1-5 by adopting a common viable bacteria flat plate counting method, wherein the number of the finished viable bacteria of the prepared micro-ecological agricultural plastic master batch and the seedling raising tray is high, and the number of the finished viable bacteria of the micro-ecological agricultural master batch is 500 plus one 1000 hundred million/g; the number of the grown live bacteria of the seedling raising tray is more than 100 ten thousand per gram.
Example 6
In this example, the seedling raising effect of the seedling raising tray prepared by the invention is studied.
The method provides a basis for exploring and researching the seedling raising effect of the seedling raising tray prepared by the invention and practicing production and application. A conventional blanket type tray is used as a control group, and a seedling raising effect comparison test is carried out. The test surveys the height, leaf age, root number, dry weight and fresh weight of the seedlings on the ground and underground.
1. Test materials
The seedling raising tray prepared by the invention
Conventional blanket type dish (market purchase)
2. Test site
Qideli farm science and technology park (Heilongjiang province city)
3. Design of experiments
The test is arranged in a greenhouse, and the test sample amount is 300 and 500 dishes. The rice variety is selected from Longjing 31 and Longjing 46, conventional seedling bed management is carried out according to the technical regulation standard of rice production, and the seedling raising effect of the seedling raising tray prepared by the method is compared with the seedling raising effect of a conventional blanket tray.
4. Survey analysis
The test treatment is carried out on a tray of 20 days in 3 months, and the seeding is carried out on 7 days in 4 months, and the observation shows that the seedling emergence time of the seedlings cultured by the seedling raising tray prepared by the invention is 12 days in 4 months, and the seedling emergence time of the seedlings cultured by the conventional blanket type tray is 14 days in 4 months. Before transplanting, the seedling raising effects of the two seedling raising trays are investigated for 5 months and 10 days, and the inspected seedling quality comprises plant height, root number, leaf age, dry weight on the ground, underground dry weight, fresh weight and the like. The average results are shown in table 1 below.
TABLE 1 survey of seedling quality
As can be seen from Table 1, the height of the seedlings cultivated by the seedling raising tray of the invention is 1.5cm higher than that of the seedlings cultivated by the conventional blanket tray, the leaf age of the seedlings is 0.2 more, the root number is 2 more, the stem base width is 0.2mm more, the fresh weight of the overground hundreds of plants is 5.2g more, and the fresh weight of the underground hundreds of plants is 1.6g more. In conclusion, the seedling height of the seedlings can be increased by adopting the seedling raising tray to raise the seedlings, and all indexes of the seedlings raised by adopting the seedling raising tray are higher than those of the seedlings raised by adopting a conventional blanket type tray. Fig. 1 is a photograph showing 12-day growth of seedlings grown using the seedling raising tray prepared according to the present invention, and fig. 2 is a photograph showing 12-day growth of seedlings grown using a conventional blanket tray. Comparing fig. 1 and fig. 2, it can be seen that the seedlings cultivated by the seedling raising tray prepared by the invention grow well and are thicker, the leaves are green oil, the leaves have no holes and are harmless to insects, and the average plant height is 5.5 cm; the seedlings cultivated by the conventional blanket type tray have poor growth vigor, are sparsely grown, have yellow leaves, occasionally have holes, and have an average plant height of 4 cm.
Fig. 3 is a photograph showing the root growth of the seedlings of the present invention raised on the tray and the conventional blanket-type tray, wherein the left is the root growth of the seedlings raised on the tray prepared by the present invention, and the right is the root growth of the seedlings raised on the conventional blanket-type tray. As can be seen by comparison, the number of the seedlings cultivated by the seedling raising tray prepared by the invention is obviously more than that of the seedlings cultivated by the conventional blanket type tray.
Fig. 4 is a photograph showing the growth of seedlings grown for 15 days using the seedling-raising tray of the present invention and seedlings grown using the conventional blanket tray, in which the growth of seedlings grown using the seedling-raising tray prepared according to the present invention is shown on the left and the growth of seedlings grown using the conventional blanket tray is shown on the right. As can be seen by comparison, the seedlings cultivated by the seedling raising tray prepared by the invention have good growth vigor, stronger seedlings and developed root systems; the seedlings cultivated by the conventional blanket type tray grow more finely, the root system is underdeveloped, and yellow leaves also appear at the roots.
FIG. 5 is a photograph showing the root growth of seedlings cultivated by the seedling tray of the present invention before transplantation. As can be seen from figure 5, the seedlings cultivated by the seedling raising tray of the invention have developed root systems and good packing effect, and are beneficial to the rapid growth of the seedlings after transplantation.
Claims (4)
1. The polyethylene micro-ecological agricultural plastic master batch is characterized by comprising the following components in parts by weight: 20-50 parts of polyethylene, 10-20 parts of bentonite, 10-30 parts of kaolin, 10-60 parts of calcium carbonate, 0.1-1 part of catalyst, 0.1-5 parts of compatilizer, 0.5-2 parts of stabilizer, 0.5-5 parts of lubricant and 0.5-5 parts of mixed probiotics;
the mixed probiotics comprise the following strains in percentage by mass: 55-60% Clostridium, 25-30% Corynebacterium and 10-16% Lactobacillus;
the clostridium comprises the following strains in percentage by mass: 18-25% of bacillus licheniformis, 35-45% of bacillus subtilis and 37-42% of bacillus amyloliquefaciens;
the corynebacterium comprises the following strains in percentage by mass: 30-40% of bacillus laterosporus and 60-70% of bacillus thuringiensis;
the lactobacillus comprises the following strains in percentage by mass: 10-20% of bacillus butyrate and 80-90% of bacillus acetate;
the stabilizer is an organotin; the lubricant is a higher alcohol;
the particle sizes of the bentonite, the kaolin and the calcium carbonate are all nano-scale;
the preparation method of the polyethylene micro-ecological agricultural plastic master batch is characterized by comprising the following steps:
1) respectively carrying out shaking fermentation culture on each pure strain;
2) centrifugally separating each pure strain subjected to fermentation culture in the step 1);
3) putting each pure strain obtained by centrifugation in the step 2) into a seeding tank for amplification culture;
4) putting each pure strain obtained by the enlarged culture in the step 3) into a fermentation pot for fermentation culture;
5) centrifugally separating each pure strain obtained by fermentation culture in the step 4);
6) mixing all pure strains obtained by centrifugation in the step 5) to obtain mixed strains;
7) absorbing and mixing the mixed bacteria with bentonite, kaolin and calcium carbonate, drying, crushing and grinding into mixed bacteria powder;
8) sequentially adding polyethylene, a catalyst, a phase solvent, a stabilizer and a lubricant into a reaction kettle, stirring for 1-5 hours at the temperature of 80-120 ℃, cooling and discharging to obtain a mixed material;
9) putting the mixed material obtained in the step 8) into a granulation extruder, and extruding and granulating to obtain the granular polyethylene micro-ecological agricultural plastic master batch.
2. The polyethylene microecological agroformoplastic masterbatch according to claim 1, wherein said catalyst is azobisbutyronitrile or cumene hydroperoxide; the compatibilizer is EVA or PEO.
3. A seedling raising tray, characterized in that the seedling raising tray is made of the polyethylene micro-ecological agricultural plastic master batch of claim 1 or 2 through extrusion molding.
4. A method for preparing a seedling raising tray as claimed in claim 3, characterized by comprising the steps of:
1) putting the polyethylene micro-ecological agricultural plastic master batch of any one of claims 1 to 3 into a calender for tabletting to obtain a sheet;
2) putting the sheet obtained in the step 1) into a seedling raising tray forming machine, and performing extrusion forming to obtain the seedling raising tray.
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| CN1052123A (en) * | 1989-10-05 | 1991-06-12 | 生物信息公司 | Biologically degradable combination film and preparation method thereof |
| CN102369105A (en) * | 2008-09-24 | 2012-03-07 | 莱斯勒斯公司 | Introduction of heat-and/or pressure-resistant organisms into materials |
| CN102766308A (en) * | 2012-08-15 | 2012-11-07 | 肖伯文 | Composite, seedling bed made of composite and preparation method of seedling bed |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1052123A (en) * | 1989-10-05 | 1991-06-12 | 生物信息公司 | Biologically degradable combination film and preparation method thereof |
| CN102369105A (en) * | 2008-09-24 | 2012-03-07 | 莱斯勒斯公司 | Introduction of heat-and/or pressure-resistant organisms into materials |
| CN102766308A (en) * | 2012-08-15 | 2012-11-07 | 肖伯文 | Composite, seedling bed made of composite and preparation method of seedling bed |
Non-Patent Citations (1)
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| 益生菌用于有机农业种植的方法;云南省科学技术情报研究院;《农村实用技术》;20141111;22 * |
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Effective date of registration: 20230801 Address after: Room 203, 2nd Floor, No. 58 Shuitou Industrial Avenue, Dali Town, Nanhai District, Foshan City, Guangdong Province, 528200 (Residence application) Patentee after: Foshan Zhichuangfulong Agricultural Technology Co.,Ltd. Address before: 528100 No. 8 Factory Building, Gekeng Village, Yuantan, Leping Town, Sanshui District, Foshan City, Guangdong Province (local name: Longgang Miaogang) Patentee before: FOSHAN YUEHONGYI BIOLOGY TECHNOLOGY CO.,LTD. |
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