CN107058126B - One plant of trichoderma asperellum and its application - Google Patents
One plant of trichoderma asperellum and its application Download PDFInfo
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- CN107058126B CN107058126B CN201710192665.0A CN201710192665A CN107058126B CN 107058126 B CN107058126 B CN 107058126B CN 201710192665 A CN201710192665 A CN 201710192665A CN 107058126 B CN107058126 B CN 107058126B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/885—Trichoderma
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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Abstract
One plant of trichoderma asperellum and its application, the present invention relates to one plant of trichoderma asperellum and its applications.One plant of trichoderma asperellum of the invention is trichoderma asperellum (Trichoderma asperellum) NECC20035, it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date is on November 16th, 2015, and deposit number is CGMCC No.11653.The application of one plant of trichoderma asperellum of the invention refers to that trichoderma asperellum (Trichoderma asperellum) NECC20035 is promoting the application on rice seedling growth while controlling disease.
Description
Technical field
The present invention relates to one plant of trichoderma asperellum and its applications.
Background technique
Rice is dry-raising and sparse-planting crop, and seedling stage is short, generally 45 days.Rice is the big grain crop in China first, but mesh
The problem of the preceding generally existing Seedlings quality difference in each rice region production in the whole nation, is mainly shown as weak seedling, sick seedling.Especially in recent years
Dry rice seedling damping-off becomes the biggest obstacle of dry-raising and sparse-planting cultivation technique, normal condition disease incidence 15% or so, because of weather, pipe
The cause of reason etc., crushing also shoe of falling ill are shown in not fresh；Rice bacterial wilt is caused by Rice Physiological dehydration, and disease often exists
Unexpected large area occurs in flakes in 1-2 days.And long-term a large amount of uses of chemical fertilizer and chemical pesticide, keep plant growth slow,
Disease is serious, and quality of agricultural product decline, essential problem is that soil degradation is serious.The method that the present invention uses trichoderma bacterial manure pouring root
Soil application processing is carried out, soil is improved, inherently solves the problems, such as rice seedling growth and Common Diseases.
Summary of the invention
The object of the present invention is to provide one plant of trichoderma asperellum and its applications.
One plant of trichoderma asperellum of the invention is trichoderma asperellum (Trichoderma asperellum) NECC20035, preservation
In China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is BeiChen West Road, Chaoyang District, BeiJing City 1
Number institute 3, preservation date are on November 16th, 2015, and deposit number is CGMCC No.11653.
The application of one plant of trichoderma asperellum of the invention refers to trichoderma asperellum (Trichoderma asperellum)
NECC20035 is promoting the application on rice seedling growth while controlling disease.
The present invention is autumn in 2013 from Harbin, Heilongjiang Province Qunli new community cloves park, lilac (Syringa
Oblata) the isolated trichoderma asperellum of rhizosphere soil (Trichoderma asperellum) NECC20035；Bacterium colony front is mitogenetic
Spore is intensive, not chromogenesis, without obvious smell；Bottle stalk on conidiophore is symmetrical；Conidium is sub- spherical.Through shape
State identifies that with ITS Molecular Identification be Trichoderma asperellum (Trichoderma asperellum), is finally named as Trichoderma asperellum
(Trichoderma asperellum)NECC20035。
Advantages of the present invention:
1, Trichoderma asperellum (Trichoderma asperellum) NECC20035 is environmentally friendly, using trichoderma bacterial manure
The method of pouring root carries out soil application processing, improves soil, inherently solves the problems, such as；
2, it can colonize in rice seedling rhizosphere, effect is obvious in a short time, and the growing environment of seedling stage high temperature and humidity, more sharp
It plays a role in Trichoderma；
It 3, is that nursery is concentrated the characteristics of Rice Seedling Culture Under Dry Condition, area is small, not only improves the fertilising of trichoderma bacterial manure, and can be reduced into
This, increases economic efficiency；
4, the trichoderma strain that the present invention obtains is the trichoderma strain of Heilongjiang Province native country separation, is applied to the cold ground in Heilongjiang Province
In seedling cultivation of rice, native country Trichoderma is in native country using adaptable, it is easier to play a role；
5, trichoderma bacterial manure pulvis is prepared using the present invention, paddy growth, and bacterial wilt and damping-off morbidity can be promoted
Rate is 0, and percentage of seedgermination improves 10%, and therefore, seed can be reduced 10% when nursery, saves seed costs 10%.
Detailed description of the invention
Fig. 1 is that rice seedling trichoderma in Suiling County handles and compares comparison diagram, and wherein A represents fertilising group, and B representative is not applied fertilizer pair
According to group；
Fig. 2 is that rice seedling trichoderma in Suiling County handles and compares single plant comparison diagram, and wherein a group represents fertilising group, and b group represents
Do not apply fertilizer control group.
Specific embodiment
Specific embodiment 1: one plant of trichoderma asperellum of present embodiment is trichoderma asperellum (Trichoderma
Asperellum) NECC20035, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation
Location is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and preservation date is on November 16th, 2015, deposit number CGMCC
No.11653。
Present embodiment is autumn in 2013 from Harbin, Heilongjiang Province Qunli new community cloves park, lilac
The isolated one plant of trichoderma asperellum (Trichoderma asperellum) of (Syringa oblata) rhizosphere soil
NECC20035；The conidium is intensive, not chromogenesis, without obvious smell；Bottle stalk on conidiophore is symmetrical；Point
Raw spore is sub- spherical.Through Morphological Identification and ITS Molecular Identification, it is Trichoderma asperellum (Trichoderma asperellum), most
It is named as Trichoderma asperellum (Trichoderma asperellum) NECC20035 eventually.
Trichoderma asperellum (Trichoderma asperellum) NECC20035 of present embodiment is environmentally friendly, adopts
Soil application processing is carried out with the method for trichoderma bacterial manure pouring root, soil is improved, inherently solves the problems, such as；And it can colonize in water
Rice rhizosphere, effect is obvious in a short time, and the growing environment of seedling stage high temperature and humidity, more conducively Trichoderma play a role；Rice In Drought Rice Nursery
The characteristics of educating is that nursery is concentrated, and area is small, not only improves the fertilising of trichoderma bacterial manure, and can be reduced cost, increases economic efficiency.This
Outside, the trichoderma strain that present embodiment obtains is the trichoderma strain of Heilongjiang Province native country separation, is applied to Heilongjiang Province cold ground water
In rice nursery, native country Trichoderma is in native country using adaptable, it is easier to play a role.
Specific embodiment 2: the application of one plant of trichoderma asperellum of present embodiment refers to trichoderma asperellum (Trichoderma
Asperellum) NECC20035 is promoting the application on rice seedling growth while controlling disease.
Using present embodiment prepare trichoderma bacterial manure pulvis, rice seedling can be promoted to grow, and bacterial wilt and stand it is withered
Sick disease incidence is 0, and percentage of seedgermination improves 10%, and therefore, seed can be reduced 10% when nursery, saves seed costs
10%.
Specific embodiment 3: present embodiment trichoderma asperellum (Trichoderma asperellum) NECC20035
Screening technique are as follows:
It weighs the lilac rhizosphere soil that 10g is acquired from Harbin, Heilongjiang Province and is placed in and fill 90mL sterile water
In 250mL sterilizing triangular flask, sufficiently vibrate.It draws in 10mL suspension and 90mL aqua sterilisa, is made 10-1Dilution.With this
Analogize be diluted to volume fraction be 10-2~10-4.It is drawn on 0.1mL suspension to rose-bengal plate with liquid-transfering gun, with sterilizing
Triangular glass smoothen, sealed with sealed membrane.It is inverted culture in 28 DEG C of incubators, separate after bacterium colony is formed pure
Change.The bacterium colony front conidium of acquisition is intensive, not chromogenesis, without obvious smell, symmetrical point of the bottle stalk on conidiophore
Cloth, conidium are sub- spherical.The DNA of Trichoderma asperellum NECC20035 extracts the CTAB method that uses, amplification wood
The ITS sequence of mould DNA directly carries out bidirectional sequencing after pcr amplification product is purified.The ITS gene order of amplification submits NCBI
Blast program analysis on (National Center for Biotechnology Information) is accredited as spine spore wood
Mould (Trichoderma asperellum) is finally named as trichoderma asperellum Trichoderma asperellum
NECC20035。
Functional authorization is carried out to the trichoderma bacterial manure pulvis of present embodiment preparation:
Test 1, detect trichoderma asperellum (Trichoderma asperellum) NECC20035 promotion paddy growth and
The ability of controlling disease:
1, using the method for trichoderma asperellum (Trichoderma asperellum) NECC20035 preparation trichoderma bacterial manure pulvis
Are as follows: PDA slant medium is prepared with polypropylen tubes first, by trichoderma asperellum (Trichoderma asperellum)
NECC20035 is inoculated on inclined-plane；15d is cultivated under conditions of room temperature, natural lighting again；Then Trichoderma asperellum spore is collected
Son mixes Trichoderma asperellum conidia powder and talcum powder 1:5 in mass ratio, then is kept in dark place to get trichoderma bacterial manure pulvis is arrived.Its
Described in polypropylen tubes specification be 3cm bore × 24cm long；The formula of the PDA culture medium are as follows: potato 200g,
Glucose 20g, agar 15g, water 1000mL.
2, paddy seedbed fertilizer test
2.1 test sites:
(1) testing site 1: Heilongjiang Province, long mountain area, Suihua City Suiling County (Jilin Provincial Military District Suileng agricultural avocation base) belongs to
Heilongjiang Province's third snow change.
(2) testing site 2: settle down Wuchang City, Heilongjiang Province peasant household, the town village De Zhe I, II, III, belongs to the product of Heilongjiang Province second
Temperate zone.
2.2 participate in the rice varieties of test
(1) rice varieties (Suiling County) cultivate rice No. 18；
(2) rice varieties (Wuchang City) the rice fragrance of a flower.
2.3 fertilization area
The sample of Suiling County 1, Wuchang City 3 sample, totally 4 differently.Each sample: fertilization area 20m2, simultaneously
20m is set2It does not apply fertilizer as control.All tests are repeated 2 times.
2.4 sowings and application date
(1) fertilising when sowing to the sample of Suiling County 1
Sowing time: on April 18th, 2015；
Sample time: on May 10th, 2015.
(2) it applies fertilizer after emerging to the sample of Wuchang City 3
Sowing time: peasant household I on April 6th, 2015；Peasant household II on April 7th, 2015；Peasant household III on April 6th, 2015.
Fertilization time: on April 22nd, 2015
Sample time: on May 6th, 2015
2.5 paddy seedbed fertilizing methods
(1) native (Suiling County) is mixed in seedbed
Dosage: 20g/m2.This bacterial manure trial product is mixed thoroughly with 1: 50 (mass ratio) ratio with fine earth.Every square metre is averagely applied
Use 1kg.
Method: being uniformly sprinkled into seedling bed for the fine earth for mixing bacterial manure, then sows blinding watering.
(2) seedbed pouring root (Wuchang City)
Dosage: 20g/m2.Bacterial manure trial product is stirred evenly with water in the ratio of 1: 200 (mass volume ratio g/mL), is obtained
High concentration reesei spores suspension is obtained, pours and applies 4L/m2。
Method: high concentration reesei spores suspension is poured in rice seedling root.
2.6 rice seedling quality determinations
(1) 22 days (- 5.10 days on the 4.18th) of fertilising
By Fig. 1 and Fig. 2 it is found that Trichoderma inducing paddy rice rice shoot is after 22 days, there is significant difference in rice seedling growth conditions.
The rice seedling of fertilising grows considerably higher compared with unmanured rice seedling, and color is greener, and grows neat.
(2) Seedling Quality in Rice changes after the induction of Heilongjiang Province Suiling County trichoderma
Seen from table 1, Heilongjiang Province Suiling County " cultivating rice No. 18 " rice seedling is after trichoderma is handled, the root of rice seedling
Length, between white number of 1.5cm, plant height, 1-2 auricle spacing, 2-3 auricle, it is 1 leaf length, 2 leaf length, 3 leaf length, total fresh weight, total
The indexs such as dry weight, aerial part fresh weight, aerial part dry weight, chlorophyll content are all remarkably higher than control (P < 0.05).Processing group
Total radical, the indexs such as stem foot is thick, leaf age, 1 leaf sheath are long indifference compared with the control group.
Seedling Quality in Rice changes after 1 Heilongjiang Province Suiling County trichoderma of table induces 22 days
(3) Seedling Quality in Rice changes after the induction of Wuchang City, Heilongjiang Province trichoderma
As can be seen from Table 2, paddy seedbed handle through trichoderma of peasant household, Wuchang City, Heilongjiang Province I " the rice fragrance of a flower " kind can make rice seedling
Total radical of seedling, plant height, 1 leaf sheath be long, between 2-3 auricle, 2 leaf length, 3 leaf length, total fresh weight, aerial part fresh weight and chlorophyll
The indexs such as content are all remarkably higher than control (P < 0.05).Leaf age, 1-2 auricle spacing, 1 leaf length, root/shoot ratio and the ground of processing group
The indexs such as upper dry weight indifference compared with the control group.
Seedling Quality in Rice changes after the induction of 2 peasant household, Wuchang City, Heilongjiang Province I trichoderma of table
Seen from table 3, peasant household, Wuchang City, Heilongjiang Province II " the rice fragrance of a flower " kind is paddy seedbed can make water after trichoderma is handled
The plant height of rice sprouts, leaf age, 1 leaf sheath be long, between 2-3 auricle, 2 leaf length, 3 leaf length, total fresh weight, aerial part fresh weight, ground
The indexs such as part dry weight and chlorophyll content are all remarkably higher than control (P < 0.05).The root long of processing group, total radical, 1.5cm
The indexs such as white root, stem foot thick, 1-2 auricle spacing, 1 leaf length indifference compared with the control group.
Seedling Quality in Rice changes after the induction of 3 peasant household, Wuchang City, Heilongjiang Province II trichoderma of table
By table 4 as it can be seen that peasant household, Wuchang City III " the rice fragrance of a flower " kind is paddy seedbed after trichoderma is handled, rice seedling it is total
Radical, plant height, stem foot be thick, 1 leaf sheath length, 1-2 auricle spacing, 2 leaf length, 3 leaf length, total fresh weight, gross dry weight, aerial part are fresh
The indexs such as weight, aerial part dry weight and chlorophyll content are all remarkably higher than control (P < 0.05).The root long of processing group, 1.5cm
The indexs such as white root, leaf age, 2-3 auricle spacing, 1 leaf length and root/shoot ratio indifference compared with the control group.
Seedling Quality in Rice changes after the induction of 4 peasant household, Wuchang City, Heilongjiang Province III trichoderma of table
From above-mentioned test it is found that the plant height of the rice seedling of 4 processing groups of the invention, total fresh weight, aerial part fresh weight with
And the indexs such as chlorophyll content are all remarkably higher than control group；The bacterial wilt and damping-off disease incidence of processing group are 0, control group
The disease incidence of bacterial wilt and damping-off is 6~7%.And the percentage of seedgermination of processing group improves 10%, seed when nursery
It can be reduced 10%, save seed costs 10%.Therefore, trichoderma asperellum of the invention (Trichoderma asperellum)
NECC20035 can promote rice seedling growth controlling disease simultaneously.
Claims (2)
1. one plant of trichoderma asperellum, it is characterised in that it is trichoderma asperellum (Trichoderma asperellum) NECC20035, is protected
It is hidden in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is BeiChen West Road, Chaoyang District, BeiJing City
No. 1 institute 3, preservation date are on November 16th, 2015, and deposit number is CGMCC No.11653.
2. the application of one plant of trichoderma asperellum as described in claim 1, it is characterised in that trichoderma asperellum (Trichoderma
Asperellum) NECC20035 is promoting rice seedling growth while preventing and treating on rice seedling blight and rice bacterial wilt disease
Using.
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| CN107384808B (en) * | 2017-09-04 | 2019-11-08 | 青岛农业大学 | Trichoderma asperellum TD3104 and its application in the microbial inoculum that preparation inhibits phytopathogen |
| CN113604364B (en) * | 2021-09-01 | 2023-08-01 | 贵州省茶叶研究所 | Trichoderma asperellum strain and application thereof |
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| CN102888350A (en) * | 2012-09-29 | 2013-01-23 | 上海交通大学 | Trichoderma strain for antagonizing soil-borne disease |
| CN102919280B (en) * | 2012-11-28 | 2014-05-07 | 东北林业大学 | Trichoderma asperellum wettable powder and applications thereof |
| CN103396954B (en) * | 2013-07-22 | 2014-11-19 | 河海大学 | Biological prevention and control bacterial strain for preventing and controlling rice sheath blight, biological organic fertilizer, and preparation method of biological organic fertilizer |
| CN105941484A (en) * | 2016-05-11 | 2016-09-21 | 江西正邦生物化工有限责任公司 | Trichoderma asperellum spore and phenazino-1-carboxylic acid compound sterilization composition |
| CN106472571A (en) * | 2016-09-22 | 2017-03-08 | 黑龙江八农垦大学 | A kind of root type bacteria agent containing ammonium humate and its using method |
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