CN107058126A - One plant of trichoderma asperellum and its application - Google Patents
One plant of trichoderma asperellum and its application Download PDFInfo
- Publication number
- CN107058126A CN107058126A CN201710192665.0A CN201710192665A CN107058126A CN 107058126 A CN107058126 A CN 107058126A CN 201710192665 A CN201710192665 A CN 201710192665A CN 107058126 A CN107058126 A CN 107058126A
- Authority
- CN
- China
- Prior art keywords
- trichoderma asperellum
- trichoderma
- plant
- rice
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/885—Trichoderma
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Abstract
One plant of trichoderma asperellum and its application, the present invention relates to one plant of trichoderma asperellum and its application.One plant of trichoderma asperellum of the present invention is trichoderma asperellum (Trichoderma asperellum) NECC20035, it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation date is on November 16th, 2015, and deposit number is CGMCC No.11653.The application of one plant of trichoderma asperellum of the present invention refers to that trichoderma asperellum (Trichoderma asperellum) NECC20035 is promoting rice seedling to grow the application on controlling disease simultaneously.
Description
Technical field
The present invention relates to one plant of trichoderma asperellum and its application.
Background technology
Paddy rice is dry-raising and sparse-planting crop, and seedling stage is short, generally 45 days.Paddy rice is the big grain crop of China first, but mesh
The problem of the preceding generally existing Seedlings quality difference in national each rice region production, is mainly shown as weak seedling, sick seedling.Especially in recent years
Dry rice seedling damping-off turns into the biggest obstacle of dry-raising and sparse-planting cultivation technique, the normal condition incidence of disease 15% or so, because weather, pipe
Cause in terms of reason, destructiveness morbidity, which is also carried out, sees not fresh；Paddy rice bacterial wilt, is that disease often exists caused by Rice Physiological dehydration
Large area occurs in flakes suddenly in 1-2 days.And chemical fertilizer and the long-term of chemical pesticide largely use, make plant growth slow,
Disease is serious, and quality of agricultural product declines, and essential problem is that soil degradation is serious.The method that the present invention uses trichoderma bacterial manure pouring root
Carry out soil application processing, improved soil, the problem of inherently solving rice seedling growth and Common Diseases.
The content of the invention
It is an object of the invention to provide one plant of trichoderma asperellum and its application.
One plant of trichoderma asperellum of the present invention is trichoderma asperellum (Trichoderma asperellum) NECC20035, preservation
In China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is BeiChen West Road, Chaoyang District, BeiJing City 1
Number institute 3, preservation date is on November 16th, 2015, and deposit number is CGMCC No.11653.
The application of one plant of trichoderma asperellum of the present invention refers to trichoderma asperellum (Trichoderma asperellum)
Applications of the NECC20035 in the growth of promotion rice seedling simultaneously controlling disease.
The present invention is autumn in 2013 from Harbin, Heilongjiang Province Qunli new community cloves park, lilac (Syringa
Oblata) the isolated trichoderma asperellum of rhizosphere soil (Trichoderma asperellum) NECC20035；Bacterium colony front is mitogenetic
Spore is intensive, not chromogenesis, without obvious smell；Bottle stalk on conidiophore is symmetrical；Conidium Asia is spherical.Through shape
State identifies that with ITS Molecular Identifications be Trichoderma asperellum (Trichoderma asperellum), is finally named as Trichoderma asperellum
(Trichoderma asperellum)NECC20035。
Advantages of the present invention：
1st, Trichoderma asperellum (Trichoderma asperellum) NECC20035 is environmentally friendly, using trichoderma bacterial manure
The method of pouring root carries out soil application processing, and improved soil inherently solves problem；
2nd, it can colonize in rice seedling rhizosphere, act on obvious in a short time, and the growing environment that seedling stage is hot and humid, it is more sharp
Played a role in Trichoderma；
3rd, it is that nursery is concentrated the characteristics of Rice Seedling Culture Under Dry Condition, area is small, not only improves the fertilising of trichoderma bacterial manure, can reduce into again
This, increases economic efficiency；
4th, the trichoderma strain that the present invention is obtained is the trichoderma strain that Heilongjiang Province native country is separated, and is applied to the cold ground in Heilongjiang Province
In seedling cultivation of rice, native country Trichoderma is in native country using adaptable, it is easier to play a role；
5th, trichoderma bacterial manure pulvis is prepared using the present invention, paddy growth, and bacterial wilt and damping-off morbidity can be promoted
Rate is 0, and percentage of seedgermination improves 10%, therefore, and seed can decrement 10%, saving seed costs 10% when nursery.
Brief description of the drawings
Fig. 1 is that Suiling County rice seedling trichoderma handles and compares comparison diagram, and wherein A represents fertilising group, and B, which is represented, not to apply fertilizer pair
According to group；
Fig. 2 is that Suiling County rice seedling trichoderma handles and compareed individual plant comparison diagram, and wherein a groups represent fertilising group, and b groups are represented
Do not apply fertilizer control group.
Embodiment
Embodiment one：One plant of trichoderma asperellum of present embodiment is trichoderma asperellum (Trichoderma
Asperellum) NECC20035, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation
Location is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and preservation date is on November 16th, 2015, and deposit number is CGMCC
No.11653。
Present embodiment is autumn in 2013 from Harbin, Heilongjiang Province Qunli new community cloves park, lilac
The isolated one plant of trichoderma asperellum (Trichoderma asperellum) of (Syringa oblata) rhizosphere soil
NECC20035；The conidium is intensive, not chromogenesis, without obvious smell；Bottle stalk on conidiophore is symmetrical；Point
Raw spore Asia is spherical.It is Trichoderma asperellum (Trichoderma asperellum), most through Morphological Identification and ITS Molecular Identifications
Trichoderma asperellum (Trichoderma asperellum) NECC20035 is named as eventually.
Trichoderma asperellum (Trichoderma asperellum) NECC20035 of present embodiment is environmentally friendly, adopts
Soil application processing is carried out with the method for trichoderma bacterial manure pouring root, improved soil inherently solves problem；And it can colonize in water
Rice rhizosphere, acts on obvious in a short time, and the growing environment that seedling stage is hot and humid, more conducively Trichoderma play a role；Rice In Drought Rice Nursery
The characteristics of educating is that nursery is concentrated, and area is small, not only improves the fertilising of trichoderma bacterial manure, cost can be reduced again, is increased economic efficiency.This
Outside, the trichoderma strain that present embodiment is obtained is the trichoderma strain that Heilongjiang Province native country is separated, and is applied to Heilongjiang Province cold ground water
In rice nursery, native country Trichoderma is in native country using adaptable, it is easier to play a role.
Embodiment two：The application of one plant of trichoderma asperellum of present embodiment refers to trichoderma asperellum (Trichoderma
Asperellum) NECC20035 is promoting rice seedling to grow the application on controlling disease simultaneously.
Trichoderma bacterial manure pulvis is prepared using present embodiment, rice seedling can be promoted to grow, and bacterial wilt and vertical withered
The sick incidence of disease is 0, and percentage of seedgermination improves 10%, therefore, and seed can decrement 10%, saving seed costs when nursery
10%.
Embodiment three：Present embodiment trichoderma asperellum (Trichoderma asperellum) NECC20035's
Screening technique is：
Weigh the lilac rhizosphere soil that 10g gathers from Harbin, Heilongjiang Province and be placed in and fill 90mL sterilized waters
In 250mL sterilizing triangular flasks, fully vibration.10mL suspension is drawn with 90mL aqua sterilisas, being made 10-1Dilution.With this
Analogize and be diluted to volume fraction for 10-2~10-4.Drawn with liquid-transfering gun on 0.1mL suspension to rose-bengal flat board, with sterilizing
Triangular glass smoothen, sealed with sealed membrane.Culture is inverted in 28 DEG C of incubators, separation is carried out after bacterium colony is formed pure
Change.The positive conidium of the bacterium colony of acquisition is intensive, not chromogenesis, without obvious smell, and the bottle stalk on conidiophore is symmetrical to be divided
Cloth, conidium Asia is spherical.Trichoderma asperellum NECC20035 DNA extracts the CTAB methods that use, amplification wood
Mould DNA ITS sequence, directly carries out two-way sequencing after pcr amplification product is purified.The ITS gene orders of amplification submit NCBI
Blast program analysis on (National Center for Biotechnology Information), is accredited as spine spore wood
Mould (Trichoderma asperellum) is finally named as trichoderma asperellum Trichoderma asperellum
NECC20035。
Functional authorization is carried out to trichoderma bacterial manure pulvis prepared by present embodiment：
Experiment 1, detect trichoderma asperellum (Trichoderma asperellum) NECC20035 promotion paddy growth and
The ability of controlling disease：
1st, the method that trichoderma bacterial manure pulvis is prepared using trichoderma asperellum (Trichoderma asperellum) NECC20035
For：PDA slant mediums are prepared with polypropylen tubes first, by trichoderma asperellum (Trichoderma asperellum)
NECC20035 is inoculated on inclined-plane；Again 15d is cultivated under conditions of room temperature, natural lighting；Then Trichoderma asperellum spore is collected
Son, by Trichoderma asperellum conidia powder and talcum powder in mass ratio 1:5 mix, then are kept in dark place, that is, obtain the fertile pulvis of Trichoderma.Its
Described in polypropylen tubes specification be 3cm bores × 24cm length；The formula of described PDA culture medium is：Potato 200g,
Glucose 20g, agar 15g, water 1000mL.
2nd, paddy seedbed fertilizer test
2.1 test site：
(1) testing site 1：The long mountain area in Heilongjiang Province Suihua City Suiling County (Jilin Provincial Military District Suileng agricultural avocation base), belongs to
The snow change of Heilongjiang Province the 3rd.
(2) testing site 2：Settle down Wuchang City of Heilongjiang Province peasant household of town De Zhe villages I, II, III, belongs to Heilongjiang Province second and accumulates
Temperate zone.
2.2 participate in the rice varieties of experiment
(1) rice varieties (Suiling County) cultivate rice No. 18；
(2) rice varieties (Wuchang City) the rice fragrance of a flower.
2.3 fertilization area
The sample of Suiling County 1, Wuchang City 3 sample, totally 4 differently.Each sample：Fertilization area 20m2, simultaneously
20m is set2Do not apply fertilizer as control.All experiments are repeated 2 times.
2.4 sowings and application date
(1) applied fertilizer when sowing the sample of Suiling County 1
Sowing time：On April 18th, 2015；
Sample time：On May 10th, 2015.
(2) applied fertilizer after emerging the sample of Wuchang City 3
Sowing time：Peasant household I on April 6th, 2015；Peasant household II on April 7th, 2015；Peasant household III on April 6th, 2015.
Fertilization time：On April 22nd, 2015
Sample time：On May 6th, 2015
2.5 paddy seedbed fertilizing methods
(1) seedbed is mixed native (Suiling County)
Consumption：20g/m2.This bacterial manure trial product is mixed thoroughly with 1: 50 (mass ratio) ratio with fine earth.Every square metre is averagely applied
Use 1kg.
Method：The fine earth for mixing bacterial manure is uniformly sprinkled into seedling bed, then sows blinding and is watered.
(2) seedbed pouring root (Wuchang City)
Consumption：20g/m2.Bacterial manure trial product and water are stirred in 1: 200 (mass volume ratio g/mL) ratio, obtained
High concentration reesei spores suspension is obtained, pours and applies 4L/m2。
Method：High concentration reesei spores suspension is poured in rice seedling root.
2.6 rice seedling quality determinations
(1) 22 days (- 5.10 days on the 4.18th) of fertilising
From Fig. 1 and Fig. 2, Trichoderma inducing paddy rice rice shoot is after 22 days, and significant difference occur in rice seedling growth conditions.
The rice seedling of fertilising, compared with unmanured rice seedling, grows considerably higher, color is greener, and grows neat.
(2) Seedling Quality in Rice changes after the induction of Heilongjiang Province Suiling County trichoderma
From table 1, after Heilongjiang Province Suiling County " cultivating rice No. 18 " rice seedling is handled through trichoderma, the root of rice seedling
Length, between the white root numbers of 1.5cm, plant height, 1-2 auricles spacing, 2-3 auricles, it is 1 leaf length, 2 leaf length, 3 leaf length, total fresh weight, total
The indexs such as dry weight, aerial part fresh weight, aerial part dry weight, chlorophyll content are all remarkably higher than control (P<0.05).Treatment group
Total radical, index indifference compared with control group such as stem foot thick, leaf age, 1 leaf sheath length.
Seedling Quality in Rice changes after the Heilongjiang Province Suiling County trichoderma of table 1 is induced 22 days
(3) Seedling Quality in Rice changes after the induction of Wuchang City of Heilongjiang Province trichoderma
From table 2, paddy seedbed handled through trichoderma of peasant household of Wuchang City of Heilongjiang Province I " the rice fragrance of a flower " kind can make rice seedling
Between total radical of seedling, plant height, 1 leaf sheath length, 2-3 auricles, 2 leaf length, 3 leaf length, total fresh weight, aerial part fresh weight and chlorophyll
The indexs such as content are all remarkably higher than control (P<0.05).Leaf age, 1-2 auricles spacing, 1 leaf length, root/shoot ratio and the ground for the treatment of group
The indexs such as upper dry weight indifference compared with control group.
Seedling Quality in Rice changes after the I trichodermas induction of 2 peasant households of Wuchang City of Heilongjiang Province of table
From table 3, peasant household of Wuchang City of Heilongjiang Province II " the rice fragrance of a flower " kind is paddy seedbed handled through trichoderma after can make water
Between the plant height of rice sprouts, leaf age, 1 leaf sheath length, 2-3 auricles, 2 leaf length, 3 leaf length, total fresh weight, aerial part fresh weight, ground
The index such as part dry weight and chlorophyll content is all remarkably higher than control (P<0.05).The root length for the treatment of group, total radical, 1.5cm
Index indifferences compared with control group such as white root, stem foot are thick, 1-2 auricles spacing, 1 leaf length.
Seedling Quality in Rice changes after the II trichodermas induction of 3 peasant households of Wuchang City of Heilongjiang Province of table
From table 4, peasant household of Wuchang City III " the rice fragrance of a flower " kind is paddy seedbed handled through trichoderma after, rice seedling it is total
Radical, plant height, stem foot are thick, 1 leaf sheath length, 1-2 auricles spacing, 2 leaf length, 3 leaf length, total fresh weight, gross dry weight, aerial part are fresh
The indexs such as weight, aerial part dry weight and chlorophyll content are all remarkably higher than control (P<0.05).The root length for the treatment of group, 1.5cm
Index indifferences compared with control group such as white root, leaf age, 2-3 auricles spacing, 1 leaf length and root/shoot ratio.
Seedling Quality in Rice changes after the III trichodermas induction of 4 peasant households of Wuchang City of Heilongjiang Province of table
It was found from above-mentioned experiment, the plant height of the rice seedling of 4 treatment groups of the invention, total fresh weight, aerial part fresh weight with
And the index such as chlorophyll content is all remarkably higher than control group；The bacterial wilt and the damping-off incidence of disease for the treatment of group are 0, control group
The incidence of disease of bacterial wilt and damping-off is 6~7%.And the percentage of seedgermination for the treatment of group improves 10%, seed when nursery
Can decrement 10%, saving seed costs 10%.Therefore, trichoderma asperellum of the invention (Trichoderma asperellum)
NECC20035 can promote rice seedling growth controlling disease simultaneously.
Claims (2)
1. one plant of trichoderma asperellum, it is characterised in that it is trichoderma asperellum (Trichoderma asperellum) NECC20035, is protected
China Committee for Culture Collection of Microorganisms's common micro-organisms center is hidden in, preservation address is BeiChen West Road, Chaoyang District, BeiJing City
No. 1 institute 3, preservation date is on November 16th, 2015, and deposit number is CGMCC No.11653.
2. the application of one plant of trichoderma asperellum as claimed in claim 1, it is characterised in that trichoderma asperellum (Trichoderma
Asperellum) NECC20035 is promoting rice seedling to grow the application on controlling disease simultaneously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710192665.0A CN107058126B (en) | 2017-03-28 | 2017-03-28 | One plant of trichoderma asperellum and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710192665.0A CN107058126B (en) | 2017-03-28 | 2017-03-28 | One plant of trichoderma asperellum and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107058126A true CN107058126A (en) | 2017-08-18 |
| CN107058126B CN107058126B (en) | 2019-11-22 |
Family
ID=59621090
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710192665.0A Active CN107058126B (en) | 2017-03-28 | 2017-03-28 | One plant of trichoderma asperellum and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107058126B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107384808A (en) * | 2017-09-04 | 2017-11-24 | 青岛农业大学 | Trichoderma asperellum TD3104 and its application in the microbial inoculum for suppressing phytopathogen is prepared |
| CN113604364A (en) * | 2021-09-01 | 2021-11-05 | 贵州省茶叶研究所 | Trichoderma asperellum strain and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102888350A (en) * | 2012-09-29 | 2013-01-23 | 上海交通大学 | Trichoderma strain for antagonizing soil-borne disease |
| CN102919280A (en) * | 2012-11-28 | 2013-02-13 | 东北林业大学 | Trichoderma asperellum wettable powder and applications thereof |
| CN103396954A (en) * | 2013-07-22 | 2013-11-20 | 河海大学 | Biological prevention and control bacterial strain for preventing and controlling rice sheath blight, biological organic fertilizer, and preparation method of biological organic fertilizer |
| CN105941484A (en) * | 2016-05-11 | 2016-09-21 | 江西正邦生物化工有限责任公司 | Trichoderma asperellum spore and phenazino-1-carboxylic acid compound sterilization composition |
| CN106472571A (en) * | 2016-09-22 | 2017-03-08 | 黑龙江八农垦大学 | A kind of root type bacteria agent containing ammonium humate and its using method |
-
2017
- 2017-03-28 CN CN201710192665.0A patent/CN107058126B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102888350A (en) * | 2012-09-29 | 2013-01-23 | 上海交通大学 | Trichoderma strain for antagonizing soil-borne disease |
| CN102919280A (en) * | 2012-11-28 | 2013-02-13 | 东北林业大学 | Trichoderma asperellum wettable powder and applications thereof |
| CN103396954A (en) * | 2013-07-22 | 2013-11-20 | 河海大学 | Biological prevention and control bacterial strain for preventing and controlling rice sheath blight, biological organic fertilizer, and preparation method of biological organic fertilizer |
| CN105941484A (en) * | 2016-05-11 | 2016-09-21 | 江西正邦生物化工有限责任公司 | Trichoderma asperellum spore and phenazino-1-carboxylic acid compound sterilization composition |
| CN106472571A (en) * | 2016-09-22 | 2017-03-08 | 黑龙江八农垦大学 | A kind of root type bacteria agent containing ammonium humate and its using method |
Non-Patent Citations (3)
| Title |
|---|
| K. NARASIMHA MURTHY等: "Induction of systemic resistance by Trichoderma asperellum against bacterial wilt of tomato caused by Ralstonia solanacearum", 《INTERNATIONAL JOURNAL OF ADVANCED RESEARCH》 * |
| SUENNY KELLY SANTOS等: "Biocontrol of sheath blight by Trichoderma asperellum in tropical lowland rice", 《AGRON. SUSTAIN. DEV.》 * |
| 陈立华等: "水稻纹枯病生防菌株的筛选及其应用方式的研究", 《中国生态农业学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107384808A (en) * | 2017-09-04 | 2017-11-24 | 青岛农业大学 | Trichoderma asperellum TD3104 and its application in the microbial inoculum for suppressing phytopathogen is prepared |
| CN107384808B (en) * | 2017-09-04 | 2019-11-08 | 青岛农业大学 | Trichoderma asperellum TD3104 and its application in the microbial inoculum that preparation inhibits phytopathogen |
| CN113604364A (en) * | 2021-09-01 | 2021-11-05 | 贵州省茶叶研究所 | Trichoderma asperellum strain and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107058126B (en) | 2019-11-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kim et al. | Growth promotion and colonization of switchgrass (Panicum virgatum) cv. Alamo by bacterial endophyte Burkholderia phytofirmans strain PsJN | |
| CN106754484B (en) | One plant of rhizobium leguminosarum and its fermentation culture method and application | |
| CN103992184B (en) | A kind of biological organic fertilizer | |
| CN102086444B (en) | Paenibacillus elgii strain and application thereof | |
| CN104263684B (en) | A kind of product siderophore series bacillus and application thereof | |
| CN106399178B (en) | Bacillus amyloliquefaciens and its application with degradation Phos and bacteriostasis | |
| CN106987541A (en) | One plant has degeneration-resistant, growth-promoting performance efficient rhizobium melioti and its application | |
| CN106399177B (en) | Bacillus amyloliquefaciens and its microbial inoculum with degradation Phos and bacteriostasis | |
| CN105670973B (en) | A kind of application of Methylotrophic bacillus, microbial inoculum, the preparation method of microbial inoculum and microbial inoculum | |
| CN106754515B (en) | Screening method of growth promoting strains for improving salt tolerance of crops | |
| CN105475363B (en) | A kind of composite bacteria agent for promoting cucumber growth and its application | |
| CN109097303A (en) | Paenibacillus polymyxa and its spore suspending liquid, microorganism seedling medium and preparation method thereof | |
| CN109251879A (en) | A kind of Jie meter La series bacillus fermentation process in high density | |
| CN108192838A (en) | A kind of bacillus amyloliquefaciens with degradation Phos and diseases prevention double action | |
| CN107904191B (en) | Rhizobium V2-2 and application thereof | |
| CN107904193B (en) | Rhizobium V14-2 and application thereof | |
| CN101519644B (en) | Sinorhizobium sp. and application thereof | |
| CN104745517A (en) | Special potassium-dissolving bacterium for tobaccos and fungicide of special potassium-dissolving bacterium | |
| CN103468591B (en) | Salt-tolerant trichoderma pleuroticola strain and application thereof | |
| CN109055274B (en) | Caragana rhizobium and fermentation culture method and application thereof | |
| CN107058126B (en) | One plant of trichoderma asperellum and its application | |
| CN109182194A (en) | One plant of Yang Ling rhizobium for promoting coronule flower growth and its cultural method and application | |
| CN107325980B (en) | Radiation-resistant paenibacillus KH9 and application thereof in biological antitranspirant | |
| CN112342173A (en) | Bacillus belgii and application thereof | |
| CN109486709B (en) | One plant of Fan Shi Halomonas bacterial strain JPT10-1 for improving corn salt resistance ability and its microbial inoculum and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |