CN107455551A - Wheat germ fermentation chromocor extract and preparation method thereof and the application in animal feeding - Google Patents
Wheat germ fermentation chromocor extract and preparation method thereof and the application in animal feeding Download PDFInfo
- Publication number
- CN107455551A CN107455551A CN201710065496.4A CN201710065496A CN107455551A CN 107455551 A CN107455551 A CN 107455551A CN 201710065496 A CN201710065496 A CN 201710065496A CN 107455551 A CN107455551 A CN 107455551A
- Authority
- CN
- China
- Prior art keywords
- wheat germ
- fermentation
- wheat
- feed
- animal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a kind of wheat germ fermentation chromocor extract and preparation method thereof and the application in animal feeding.The wheat germ fermentation chromocor extract of the present invention extracts from wheat germ fermented supernatant fluid to be obtained；Preferably purify what is obtained through nonpolar or low pole macroporous resin column chromatography using wheat germ fermented supernatant fluid, wherein general flavone content is 2.5 5.8mg/g dries.Wheat germ fermentation chromocor extract can be used as substitute antibiotics feed addictive to be used to prepare production feed or premixed feed, Animal diseases are prevented and treated, improve letting animals feed yield, improve vaccinated animals antibody titer, extend effective titre in antibody blood, enhancing inoculation animal is to disease resistance.
Description
Technical field
The invention provides a kind of wheat germ fermentation chromocor extract and preparation method thereof and the application in animal feeding.
Background technology
Nineteen forty-six, Moore etc. publish thesis discuss first in broiler fodder add antibiotic can improve productivity
Can, antibiotic is added to promote growth of animal to come into vogue in feed from this.First, antibiotic, which turns into animal feed, to be added
Agent.The invention and application of antibiotic are one of greatest inventions of twentieth century, and the use of antibiotic was once established for large-scale cultivation
Determined basis, but the abuse of antibiotic has triggered serious side reaction, bacterial drug resistance go out serious threat to the mankind and
The safety of animal.To prevent and cure diseases, domestic animal/fowl, fish feed is extensively using a large amount of antibiotic, meat, medicine in egg and dairy produce
Residual triggers the worry of people, rabid ox disease already, and bird flu is even more to exacerbate the food-safe worry of people, expects organic peace
Total eclipse product turn into social consensus already.1986, Sweden took the lead in starting to disable antibiotic in animal feed；In January, 2006, Europe
Man of ally completely forbids uses antibiotic in poultry feed, and U.S. FDA regulation was completely forbidden in poultry feed from 2016
It is middle to use antibiotic.Though China disables antibiotic comprehensively not yet in feed at present, anti-by what can be added in feed
It is several that raw plain kind by kind more than 50 is reduced to 20.As can be seen here：Antibiotic is limited the use of/disabled in feed has evolved into history tide
Stream.
Antibiotic is added in feed mainly two functions：First, regulation and control enteric microorganism；Second, the supply that has additional nutrients, just
It is to make the thinning absorption that has additional nutrients of intestinal cell wall.Researched and developed around antibiotic substitute products, scientific worker largely grind
Study carefully work and achieve certain progress, this kind of additive mainly has following three class：
1. acidulant：Acidulant one is to affect indirectly microorganism species, second, by sterilization, suppresses inhereditary material and replicates
Directly affected with cell membrane is destroyed.Single acidulant, such as formic acid, propionic acid；Fumaric acid and the very high (8- of citric acid addition
25KG/ tons).Compound-acid application effect is preferable.European Union's pig industry uses Compound-acid substitute antibiotics, be disadvantageous in that dosage compared with
Greatly, high expensive.
2. probiotics：Probiotics is one of main fodder antibiotics substitute.But due to the complexity of product
Property and research difficulty it is unsatisfactory in terms of industrialization at present.Effective probiotics will answer two problems：When
It could prove that enteron aisle can be reached by stomach by isolating beneficial microbe, and in intestinal colonisationSecond, it could prove this beneficial
Microorganism can suppress/and straw are anti-/ or even kill pathogenic microorganismIn addition viable bacteria formulation, preserve valid density viable bacteria the problems such as one
Straight puzzlement probiotics industrialization.Such product that the country has been enter into feed addictive catalogue at present has 16 kinds.But with gemma
Bacillus；Based on saccharomycete and lactic acid bacteria.
3. plant extracts：Plant extracts is one of main fodder antibiotics substitute.European Union is to forbid in the world
Using earliest, the maximum and most tight region of fodder antibiotics, European Union successfully disables fodder antibiotics, good except having benefited from
Beyond feeding management supplementary measures, a highly important material measure is to be substituted feed with plant extracts (Chinese herbal medicine) and used to resist
Raw element, in successfully fodder antibiotics are disabled, it is important that Chinese herbal medicine has played first.European Union is successful application feed Chinese herbal medicine
Sole zone.There is the problem of unstable in Chinese herbal feed additive, come from the application：Growth of animal condition difference, prescription
Difference and production technology difference.Chinese herbal feed additive product quality is very different on the market, problems be present：1. medium-height grass
Medicine compatibility and the mechanism of action is not also very apparent, and Chinese herbal medicine improper use, also there is toxicity not entirely without toxicity, side effect
It is even fatal.Further, since compound Chinese herb functional component is complicated, wherein certain composition there may be with other feed addictives
Antagonism.Therefore safety test, this respect work quite weakness are being first passed through before；2. Chinese herbal feed additive produces
How coarse product are, is the mixture of Chinese herbal medicines through simply processing, and dosage is big, it is slow to work, of poor quality, it is difficult to adapt to animal husbandry scale
Change, the needs of intensive modern production；3. lack the industry or national standard of Chinese herbal feed product, Chinese herbal medicine formula also five
Hua Bamen, lack scientific, quality testing without according to can according to, very big randomness be present.In view of above present situation, develops new, quality
It is very urgent that controllable and without potential safety hazard green antibiotic substitutes feed addictive.
Wheat is one of staple food crop in the world.The wheat total output in China accounts for No. 1 in the world, gross annual output amount
About 1.10 hundred million tons, up to 2,800,000 ton -420 ten thousand tons of the hiding amount of the wheat embryo that can be developed.Wheat embryo is produced per year at present
Amount contains extremely abundant and high-quality protein, fat up to the source of life that 30,000 tons~50,000 tons of wheat embryos are wheat seeds
Fat, multivitamin, mineral matter and some micro physiologically active ingredients, " the natural nutrition treasured of the mankind is described as by nutritionists
Storehouse ", " Source of life of the mankind ".The matter of flour finished product is influenceed because of the high ash content of wheat embryo, color and luster, rich in fat and enzyme
Amount, so, plumule is often removed when processing flour, is thrown away as leftover bits and pieces or is enriched as feed wheat embryos in vain
Nutrition and health care composition and its abundant active material protrusion physiological function, more and more by domestic and international research and development person's
Pay attention to.However, though China possesses extremely abundant wheat embryo resource, research and development and utilization in this regard are very slow
Slowly.
Wheat embryo is put into country always《Feedstuff catalogue》(1.11.11) is from the point of view of nutritional point, as feeding
Material, wheat germ should be one of most excellent feed, but wheat germ due to containing abundant grease and various enzymes (such as protease) and
ANFs, very easy at normal temperatures putrid and deteriorated, these are all the reason for influenceing wheat germ extensive use.
Saccharomyces cerevisiae (Sccharomyes cerevisiae) is unicellular microorganism, belongs to fungi, and acid resistance is strong, is simultaneous
Property anaerobic bacteria.Active dry yeast；Yeast culture；Yeast extract and the Ministry of Agriculture of yeast cell wall Deng Doushi China《Feed adds
Add agent kind catalogue》(2008) feed addictive used is allowed.Saccharomycete protein content itself is up to 40-50%, remote high
In high-quality feeds such as soybean；Yeast Cultivation process, which produces a large amount of secondary metabolites, includes organic acid；Amino acid, polypeptide, nucleosides
Acid and vitamin etc..Yeast product can improve livestock and poultry alimentary tract colony balance, strengthen immunity of organisms；Abundant nutrients is provided
Matter, so as to reach preventing and treating disease of digestive tract and promote the multiple actions such as growth, have nontoxic；Have no side effect, it is pollution-free, do not produce
The features such as resistance to the action of a drug.It is a kind of green feed addictive.
Wheat germ and saccharomyces cerevisiae have been the feed addictive of very excellent feedstuff or substitute antibiotics in itself.The two
Fermented by bioengineering, wheat germ and yeast can produce many new active materials during the fermentation, such as in wheat germ it is more
The activation under saccharomycete effect of kind enzyme produces various active albumen；New Flavonoids of wheat gem or anthraquinone analog compound.It has been proven that：
Such active material can suppress various pathogens in vivo and in vitro, improve body's immunity, stimulate bone-marrow-derived lymphocyte, and T lymphs are thin
Born of the same parents；NK natural killer cells, promote cytokine profiles secretion (IL-2, IL-6, TNF, INF etc.).
Wheat germ extractive from fermentative is used for cancer patient food additives more than ten years by Hidvegi, Mate, through it is international more in
Heart clinical verification effect clearly, the American-European (patent No. of the Product Marketing at present：WO99/08694).Using with health food phase
Same zymotechnique, they have studied wheat germ extractive from fermentative as poultry ＆ feed additive for fowls substitute antibiotics and work simultaneously
For the feasibility (patent No. of veterinary drug application：WO2004/014146).As a result prove：Using 0.05-0.3% (0.5-3KG/ tons)
Wheat germ extractive from fermentative, and compared using the control group of antibiotic, piglet, getting fat pig, broiler chicken, laying hen, turkey can be significantly improved
Animal disease and the death rate are reduced with goose feed conversion ratio (5-10%) simultaneously., can be significantly using wheat germ extractive from fermentative
Degree improves vaccine inoculation chicken antibody and produces titre.Tested as veterinary drug, the product can prevent and/or treat the global-worm illness sense of poultry
Dye, prevention and/or treatment porcine mycoplasmal infection, reduce family's Mycoplasma gallisepticum or mycoplasma fluid infection.However,
WO2004/014146 is the simple zymotechnique extraction wheat germ extractive from fermentative using health food, causes wheat germ to ferment
Nucleus content in extract is relatively low.
A large amount of in vitro studies in vivo also have proven to：Wheat germ fermented product has antibacterial, anti-oxidant, and killing/suppression tumour is thin
The physiologically active such as born of the same parents, regulation body's immunity.These physiologically actives are adjusted to the mankind and Immune Function In Animals, and disease prevention and cure are equal
There is very positive meaning.Wheat germ extractive from fermentative composition is sufficiently complex, core active composition in relevant wheat germ fermenting mixture
Research is more active.Early stage Hidvegi, Mate research think that 2,6- dimethoxy-p- benzoquinones (2,6DMBQ) carries for wheat germ fermentation
Take the core active composition of thing and apply for a patent and be protected by (trade name Avemar).2,6-DMBQ has very strong anticancer in vitro
Effect, but in vivo studies fails to confirm that the compound has immunological regulation and anticancer function.Avemar is wheat germ fermentation extraction
Thing adds the mixture of excipient.
The content of the invention
In the prior art, improving food conversion ratio, reducing mortality of animals is added by professional mixed feed and feed
Add agent to include antibiotic to realize, and disable/limit the use of antibiotic at present as feed addictive into social consensus, the present invention
It is to seek to be applied to animal feeding using natural organic feed additive substitute antibiotics under this background.
It is an object of the present invention to provide a kind of new wheat germ fermentation chromocor extract.
Another object of the present invention is to provide a kind of preparation method of new wheat germ fermentation chromocor extract.
Another object of the present invention is to the feature using the legal Flavonoids of wheat gem extract of HPLC fingerprint chromatograms, general flavone
Determine general flavone content in standard measure extract product.
Another object of the present invention is to study fermentation Flavonoids of wheat gem using extracorporeal culturing method animal peripheral blood is immunized
The influence of cell, while wheat germ fermentation flavones and wheat germ extractive from fermentative are compared in vitro to immunocyte and cell factor point
The influence secreted.Wheat germ extractive from fermentative is that purified concentrated spray is not dry and obtain for wheat germ fermenting mixture.
The change of breeder antibody level is followed by another object of the present invention is to observe inoculation chicken vaccine.Observed result table
It is bright：Wheat germ fermentation flavones is added in chicken feed, chicken antibody production can be dramatically increased compared with control group, improves antibody level,
The cultivation phase strengthens chicken vaccine effect, and the protection of higher degree is provided for inoculation animal.
Another object of the present invention is to ferment, Flavonoids of wheat gem is applied to as a kind of brand-new multifunctional feed additive
The large-scale farming of the animals such as domestic animal/poultry/fish.In large-scale cultivation, feed cost, mortality of animals is to concern enterprise
One of principal element of breeding income.The research of the present invention finds that wheat germ fermentation flavones can be applied to animal with flying colors and raise very much
Support.Wheat germ fermentation protein can improve food conversion ratio, and promote the weight of animals more rapidly to increase, and improve resistance of the animal to disease
Power, the especially resistance to various infectious diseases.It is particularly suitable for improving under the conditions of large-scale production the production of letting animals feed meat
Amount, improve the quality of meat.
On the one hand, the present invention isolates and purifies out wheat germ fermentation mixing using modern biotechnology from wheat germ fermented supernatant fluid
Core active principle-Flavonoids of wheat gem in thing, obtain a kind of wheat germ fermentation chromocor extract.
In the present invention, flavones sizing technique control wheat germ fermentation chromocor extract product quality can be added with fingerprint chromatogram method.
In the present invention, using inside and outside Experimental comparison prove fermentation Flavonoids of wheat gem be ferment wheat germ mixture in core have
Imitate component.And Flavonoids of wheat gem of fermenting is applied to the raising and prevention from suffering from the diseases of domestic animal, poultry.
Flavones is widely present in most plants, and bioactivity is very extensive.Wheat flavones is mainly distributed on wheat
Bud and wheat bran, wheat germ powder is raised more than decades of times with flavones content after fermentation by saccharomyces cerevisiae and huge change also occurs for bioactivity
Change.The present invention isolates and purifies fermentation Flavonoids of wheat gem using chromatographic technique from wheat germ zymotic fluid, obtains the wheat germ fermentation of the present invention
Chromocor extract.
According to specific embodiments of the present invention, present invention firstly provides a kind of wheat germ tunning, wheat germ fermentation
Product is prepared in accordance with the following methods：
Wheat germ powder, food yeast add water mixed fermentation, fermentation period 15~72 hours, preferably 24~48 hours, after fermentation
Obtain the wheat germ tunning of the present invention.
According to specific embodiments of the present invention, in the preparation of wheat germ tunning of the invention, fermentation temperature 26~36
DEG C, preferably 30 DEG C.The weight of wheat germ and yeast ratio is 2:1 to 8:1, preferably 5:1.In fermentation system, the ratio of dry and water
For 1:7~1:14, preferably 1:8~1:10.
, can in the preparation of wheat germ tunning of the invention, during large scale fermentation according to specific embodiments of the present invention
Continuous ventilation as needed, is slowly stirred, plate-frame filtering after fermentation ends.It can also be added as needed in fermentation process appropriate
Defoamer.
According to specific embodiments of the present invention, present invention also offers a kind of wheat germ fermentation-product supernatant, its be by
Fermented supernatant fluid after the wheat germ tunning filtering of the present invention.Preferably, it is by plate-frame filtering, filters out naked eyes visible solid
Material and mechanical admixture.Appropriate filter aid can be added during filtering as needed.The filter cake obtained in the present invention, it can be further dried
And it is granulated.
According to specific embodiments of the present invention, wheat germ of the invention fermentation chromocor extract is to prepare in accordance with the following methods
Obtain：
By the wheat germ fermented supernatant fluid after plate-frame filtering by the macroreticular resin for preparing (should during, the fermentation such as flavones
Wheat germ active material overwhelming majority material is adsorbed in nonpolar or low pole macroreticular resin, and other things such as starch, polysaccharide, albumen
Matter flows out chromatographic column with liquid, i.e., starch, polysaccharide, protein mixture after being fermented present invention also offers these wheat germs, this portion
Mixture is divided to account for fermentation supernatant solids gross weight 75~85%)；Wash that (such as 90% ethanol can be disposable with ethanol is de- afterwards
More than 96% adsorbate is all parsed, or uses other concentration ethanol gradient elutions) active material of macroreticular resin is adsorbed in,
Obtain the wheat germ fermentation chromocor extract of the present invention.
According to specific embodiments of the present invention, in the preparation of wheat germ of the invention fermentation chromocor extract, macroreticular resin
For D101 non-polar macroporous resins.Preferably, the wheat germ ferment filtrate of the adsorbable 10 times of volumes of D101 macroporous resin columns.
According to specific embodiments of the present invention, described eluted product further vacuum-concentrcted can be dried, and obtain
The wheat germ fermentation chromocor extract of solid-state.
Flavones content in the wheat germ fermentation chromocor extract product prepared using the above-mentioned fermentation of the present invention and purification process
For 2.5-5.8mg/g dries.
The wheat germ fermentation chromocor extract prepared using the above-mentioned fermentation of the present invention and purification process is accounted for fermentation filtrate and consolidated
About 13~17% or so of body composition.
Material property of the present invention can be described with two kinds of hair methods, that is, pass through fingerprint chromatogram (Fingerprint
Chromatogram) or/and general flavone content detection is (in terms of rutin).The former is the qualitative analysis to Flavonoids of wheat gem of fermenting, after
Person is then quantitative analysis.
Fingerprint chromatogram is carried out using HPLC methods.Hplc device condition：Agilent C18 posts, long 150*4.6mm is leant on, detected
Wavelength 254nm, 30 DEG C of column temperature.Condition of gradient elution such as table 1, isolates seven characteristic peaks under the separation condition.6.8min；
7.25min；8.5min；8.8min；9.5min；11min；16.5min.
Wheat germ fermentation purification of samples general flavone detection method uses<<Health food is examined and assessment technique specification>>(2003
Year) the version Part II method of inspection 24 total flavonoids in health food measure.Wheat embryo general flavone content is in 22-
80mg/100g, flavones content significantly raises more than ten times after the fermentation of wheat germ yeast.
It is used to match somebody with somebody as substitute antibiotics feed addictive present invention also offers described wheat germ fermentation chromocor extract
The application of system production feed or premixed feed.
In the wheat germ fermentation chromocor extract application of the present invention, antibiosis is substituted with fermentation Flavonoids of wheat gem in the animal feed
Element is as feed addictive preventing and treating Animal diseases.
In the wheat germ fermentation chromocor extract application of the present invention, the animal feed containing wheat germ fermentation chromocor extract
Available for raising letting animals feed yield.
In the wheat germ fermentation chromocor extract application of the present invention, the animal feed containing wheat germ fermentation chromocor extract
Available for vaccinated animals antibody titer is improved, extend effective titre in antibody blood, enhancing inoculation animal is to disease resistance.
In the wheat germ fermentation chromocor extract application of the present invention, the animal containing wheat germ fermentation chromocor extract is raised
Material, the animal is ox, horse, sheep, pig, chicken, duck, goose, rabbit and cultured fishes.
In the wheat germ fermentation chromocor extract application of the present invention, the animal containing wheat germ fermentation chromocor extract is raised
Material, fermentation Flavonoids of wheat gem usage amount is in 0.005-2g/kg feeds, preferably 0.05-0.5g/kg feeds.
In the wheat germ fermentation chromocor extract application of the present invention, wheat germ fermentation flavones can be used for the anti-of a variety of diseases of animal
Control, such as infective inflammation, mycoplasma infection, pig pneumonia, coccidiosis of domestic fowls infection etc..
In wheat germ fermentation chromocor extract application of the invention, fermentation Flavonoids of wheat gem can be individually oral or injected ill dynamic
Thing, it may be added in feed, dosage should be improved moderately.
The wheat germ fermentation chromocor extract of the present invention can be used for the extensive raising of porkling, getting fat pig, chicken, and trial uses wheat
Embryo fermentation flavones substitute antibiotics.Found in research：Flavonoids of wheat gem of fermenting was raised to weaned piglets, getting fat pig and the whole of chicken
Cheng Jun is favourable, and it improves increase and the efficiency of feed utilization of the weight of animals, enhances animal subject resistance, and it is dynamic to reduce raising
The thing death rate.Control group contrast effect more good job is used with Conventional antibiotic.
Simultaneously thrilling is that wheat germ fermentation flavones and wheat germ extractive from fermentative can activate pig peripheral blood T leaching in vitro
Bar cell, bone-marrow-derived lymphocyte, NK natural killer cells, macrophage, immunocyte is stimulated to discharge interleukin 2, leucocyte
The cytokine profiles such as interleukin -6 and interferon.Under same culture conditions, wheat germ fermentation flavones needed for activating immune system cell
Concentration is compared with low ten times or so of wheat germ extractive from fermentative concentration, the core of experimental result prompting wheat germ extractive from fermentative regulation immunocyte
Heart active ingredient is mainly wheat germ fermentation flavones.
Brief description of the drawings
Fig. 1 is fermentation Flavonoids of wheat gem fingerprint chromatogram.
Fig. 2 is that D101 macroreticular resins flow out zymotic fluid chromatogram.
Fig. 3 shows secretory cell results of the IFN/IL-2 in CD4 (Th1) helper lymphocyte.
Fig. 4 shows the secreting, expressing result of TNFg/IL-6 in monocyte.
Fig. 5 shows CD69 in NK cell activation results.
Embodiment
In order to which technical characteristic, purpose and the beneficial effect of the present invention is more clearly understood, in conjunction with specific implementation
Example and accompanying drawing to technical scheme carry out it is described further below, it should be understood that these examples be merely to illustrate the present invention without
For limiting the scope of the present invention.In embodiment, each Starting reagents material is commercially available, the experiment of unreceipted actual conditions
Method is conventional method and normal condition known to art, or according to the condition proposed by apparatus manufacturer.
The present invention isolates and purifies fermentation Flavonoids of wheat gem using chromatographic technique from wheat germ zymotic fluid.
Wheat germ powder, food yeast add water mixed fermentation, preferably fermentation period 15~72 hours, 24~48 hours.Fermentation temperature
26~36 DEG C, preferably 30 DEG C of degree.The weight of wheat germ and yeast ratio is 2:1 to 8:1, preferably 4:1~5:1.The ratio of dry and water
Example is 1:7~1:14, preferably 1:8~1:10.It should continuously ventilate, be slowly stirred during large scale fermentation, sheet frame mistake after fermentation ends
Filter.
Wheat germ fermented supernatant fluid after plate-frame filtering is fermented wheat germ active material by the macroreticular resin, flavones etc. prepared
Most materials are adsorbed in the other materials such as nonpolar or low pole macroreticular resin, starch, GL-PP and flow out layer with liquid
Analyse post.90% ethanol is de- to wash the fermentation Flavonoids of wheat gem for being adsorbed in macroreticular resin.Vacuum-concentrcted dries fermentation Flavonoids of wheat gem.Profit
The product flavones content prepared that ferments and purify in aforementioned manners is 2.5-5.8mg/g dries.
Embodiment 1, wheat germ fermentation pilot scale
40kg wheat germ powders and 10kg yeast are poured into 500 liter fermentation tanks, add water to 400 liters, fermentation time 48 hours,
Continuously ventilate (16-18 cubic meters/hour) and stir (100 revs/min) during fermentation, tank pressure 0.05P.To suppress foam generation culture
0.4 liter of defoaming spirit is added in thing.Appropriate filter aid, subsequent plate-frame filtering are added after fermentation ends in fermenting mixture.Collect
Plate-frame filtering liquid is in case next stage chromatographic purifying.
Sheet frame filter cake is collected, dries and is granulated in fluidization drying apparatus, particle diameter is adjusted to 0.3-0.5mm.It is dry
The filter cake of dry granulation is labeled as component 1.
Embodiment 2, macroreticular resin one-step method purification of fermentation Flavonoids of wheat gem
Post is filled after the pretreatment of D101 non-polar macroporous resins, by the plate-frame filtering liquid Column chromatography of embodiment 1, from chromatographic column stream
The liquid colorless gone out, generally, the wheat germ ferment filtrate of the adsorbable 10 times of volumes of D101 macroporous resin columns.Collect from macropore
Resin trickle, after being concentrated under reduced pressure plus excipient spray drying, drying sample are labeled as component 2.Component 2 accounts for fermentation supernatant and consolidated
Body thing gross weight 80%.
With 1 times of column volume deionized water rinsing adsorption column after end of the sample, the wheat germ hair being adsorbed on D101 macroreticular resins
Fermentation compound can be selected that different graded ethanol concentration are de- to wash sample, for example 30% ethanol, 50% ethanol are finally taken off with absolute ethyl alcohol
Wash sample, different compounds are different to D101 macroporous resin adsorptions intensity, whereby can component needed for purification enrichment to greatest extent.This
One purification process provides effective means to separate effective medicinal components from wheat germ extractive from fermentative.Wheat germ fermentation of the present invention is yellow
Ketone is de- to be washed using 90% ethanol as de- washing lotion, disposably all parses more than 96% adsorbate.Collect that 90% ethanol is de- to be washed
Liquid, be concentrated under reduced pressure drying sample.Drying sample is labeled as component 3, and component 3 accounts for about the 15% of fermentation filtrate solid constituent.Group
3 dry general flavone contents of part are 2.5-5.8%.
Embodiment 3, material property of the present invention description
Material property of the present invention can be described with two kinds of hair methods, that is, pass through fingerprint chromatogram (Fingerprint
Chromatogram) or/and general flavone content detection is (in terms of rutin).The former is the qualitative analysis to Flavonoids of wheat gem of fermenting, after
Person is then quantitative analysis.
Fingerprint chromatogram is carried out using HPLC methods.
Sample preparation：500mgD101 macroreticular resins elution samples (component 3) are dissolved in 20 milliliters of absolute methanols, 200 turns/
Divide concussion dissolving 30 minutes, 3000 revs/min of sample centrifuges 5 minutes, injects upper prop after 0.22 zut filter sample, sample is dense
Spend 25mg/ml.HPLC appointed conditions：Agilent C18 posts, long 150*4.6mm is leant on, detect wavelength 254nm, 30 DEG C of column temperature.Gradient
Elution requirement such as table 1 below：
The condition of gradient elution of table 1
| Time (min) | Flow velocity (ml/min) | 0.05% aqueous formic acid | The 0.05% fine solution of formic acid second |
| 0 | 2 | 95 | 5 |
| 1 | 2 | 95 | 5 |
| 15 | 2 | 70 | 30 |
| 15.5 | 2 | 5 | 95 |
| 17.5 | 2 | 5 | 95 |
| 18 | 2 | 95 | 5 |
| 20 | 2 | 95 | 5 |
Fig. 1 fermentation Flavonoids of wheat gem fingerprint chromatograms.
Fig. 1 shows fermentation Flavonoids of wheat gem fingerprint chromatogram result, and seven characteristic peaks are isolated under above-mentioned separation condition.1；
6.8min.2；7.25min；3；8.5min；4；8.8min；5；9.5min；6；11min；7；16.5min.
Fig. 2 is that D101 macroreticular resins flow out zymotic fluid chromatogram, i.e., is not inhaled from the wheat germ fermentation supernatant of macroreticular resin outflow
Attached component chromatogram, this component materials eluted in 2 minutes from C18 posts to flow out, wheat germ fermentation supernatant solids 80% in this group
Divide (component 2), the bioactivity of component 2 is very low.
Wheat germ fermentation purification of samples general flavone detection method uses<<Health food is examined and assessment technique specification>>(2003
Year) the version Part II method of inspection 24 total flavonoids in health food measure.Wheat embryo general flavone content is in 22-
80mg/100g, flavones content significantly raises more than ten times after the fermentation of wheat germ yeast.
Embodiment 4, Flow cytometry fermentation Flavonoids of wheat gem processing animal peripheral blood intracellular cytokine expression and
The activation of immunocyte.
Cell factor is a kind of more peptide or proteins with bioactivity, closely related with immune response, inflammatory reaction.It is auxiliary
Helping property Th1 (CD4) cell secretes IL-2 and IFN and participates in cellular immunity, Th2 (CD8) and the secretion of monocyte (CD11c) cell
IL-4, IL-6, IL-10 and TNF are primarily involved in humoral immunity.
The present embodiment is sterile to take healthy pig peripheral blood, and lymphocyte separation medium separation prepares single cell suspension (1X10^6
Cell/ml) in 1640 complete culture solutions, experimental group added in cell culture fluid 25 micrograms fermentation Flavonoids of wheat gem (component 3), 37
DEG C, 5%CO2Incubator culture 12 to 24 hours, control group only add the solvent of component 3.Culture is normal by flow cytometry after terminating
Rule require processing cell and add corresponding fluorescence antibody.Detected using FACScan flow cytometers, each sample detection
2000 cells, data analysis use Lysis II softwares.As a result Fig. 3, Fig. 4 are seen.
Fig. 3 results are visible, and untreated fish group IFN/IL-2 expression is very low (0.04%/0.03%), and ferment wheat germ
Flavones (component 3) handle pig peripheral blood after 12 hours both secretory cell numbers in Th1 helper lymphocytes it is significantly raised
(IFNg raises 168-280 times, and IL-2 raises 24-134 times).
Fig. 4 results show component 3 and after pig peripheral blood culture 12-24 hours, TNFg/IL-6 expresses secretion in monocyte
Cell quantity raised 113/7 (TNF/IL-6) and 278/37 times at 12 hours and 24 hours respectively.
Fig. 5 Flow cytometries wheat germ extractive from fermentative (250 micrograms/ml) and wheat germ ferment, and (25 is micro- for flavonoid component 3
Gram/ml) and handle pig peripheral blood monocyte 24 hours, flow cytomery natural killer cell (NK cells) activation ratio.
As a result show：Control group CD16+NK cells 29%；CD3+T cells 2.49%, wheat germ extractive from fermentative treatment group CD16+NK are thin
Born of the same parents are improved to 51.4% up to 96.6%, CD3+T cells.Wheat germ fermentation flavones (component 3) CD16+NK cells 97.52%, CD3+T
Cell 63.5%.This result of study is prompted：Wheat germ fermentation flavones/extract can strong activating animals natural killer cell.Hair
Concentration needed for ferment Flavonoids of wheat gem activation NK cells is sent out compared with 10 times of extract less than wheat germ, is speculated accordingly, and fermentation Flavonoids of wheat gem should be
Adjust the core active component of the bioactivity such as immunocyte.
Fermentation Flavonoids of wheat gem activation pig peripheral blood flow cytomery result is recorded in table 2.
The fermentation Flavonoids of wheat gem activation pig peripheral blood flow cytomery result of table 2
Embodiment 5, fermentation Flavonoids of wheat gem enhancing bursal disease live vaccination chicken antibody are horizontal
Bursal Disease (IBD) is a kind of young chicken high degree in contact viral infectious, causes young chicken mainly to be exempted from
The epidemic disease organ bursa of farbricius is impaired to trigger immunity of organism to suppress, and increases the neurological susceptibility to Other diseases, great threat is caused to poultry husbandry,
Vaccine inoculation is to prevent the sick effective means.Occur again and again because chicken individuals produce antibody level difference immuning failure.
60 10 age in days children chickens are randomly divided into four groups, every group 15.A groups：Add (0.05 gram/public of Flavonoids of wheat gem group in feed
Jin feed)；B groups：The control group raised by chow diet：Two groups of A, B inocalation method times spirits on the day of packet, dosage is 1.5 plumages
Part.C groups：Raised with A groups but inocalation method times is not clever；D groups：Raised with B groups but inocalation method times is not clever.Four groups of animals are 0；7；
14；Take a blood sample within 21 and 28 days, (IDEXX Products) are detected with IBD ELISA antibody assay kits.Entered with SPSS statistical softwares
Row significance difference analysis.Experimental result is shown in Table 3.
Influence of the fermentation Flavonoids of wheat gem of table 3 to antibody titer after young chicken inoculation IBD vaccines
The result of table 3 is prompted：Each group maternal antibody titre is higher when being inoculated with 0 day, and chow diet is raised and is not inoculated with IBD
The control group (D groups) of vaccine reduces rapidly with time lengthening antibody titer after inoculation, and antibody concentration is minimum at 28 days.With D groups pair
Than being always maintained at higher level though not being inoculated with IBD vaccine feeds and adding fermentation Flavonoids of wheat gem group maternal antibody titre, counting
Processing shows 7 to 28 days two groups of antibody titers, and there were significant differences.It is inoculated with IBD vaccines but raising chow diet group (B groups) is anti-
Body titre was progressively raised to the 1537.A groups chicken of 28 days from 475 after the 7th day and antibody titer does not almost occur and substantially reduces, with B groups
Control group compares, and antibody titer is always 2-4 times of control group, statistical procedures difference highly significant.
Embodiment 6, raised table hens experiment using addition fermentation Flavonoids of wheat gem feed
Choose 1600 1 age in days U.S. Ai Weiyin white meat-type chickens and be randomly divided into tetra- groups of A, B, C, and D, every group 400.Raising
The basal diet of same recipe.A groups are control group, and B groups are that complete feed group (contains antibiotic, vitamin and trace element add
Agent)；C groups are that 0.05 gram of fermentation Flavonoids of wheat gem/kg diet is added in basal diet；D groups are to be added in basal diet
0.35 gram distributes full ferment wheat germ powder (fermentation wheat germ component 1+ component 2+ components 3)/kg diet.Feeding management is according to NY/
T5038；5036；5035 and 5037 standards.Periodically anti-epidemic measure is taken by correlation epidemic prevention regulation.The age in days of experimental chicken 45 terminates space-time
Abdomen claims quality, calculates day increment, daily ingestion amount, case fatality rate and feed conversion rate etc..
Every group takes 6 chicken bloodletting to slaughter dissection taking-up immune organ respectively：The bursa of farbricius；Both sides thymus gland and spleen, reject fat
Fat tissue and being blotted with blotting paper is weighed on organ after watery blood, determines immune organ quality and index.
Immune Organs Index=immune organ quality/live chickens quality.
Experimental group B as seen from Table 4；C and D groups are respectively increased 22.2% than control group (A) quality that increases day by day respectively；24.8% He
21.7. statistical procedures significant difference, B, C, D respectively test group difference not significantly (P>0.05).
Each experimental group broiler chicken quality growth pattern of table 4 compares
| Group | Initial mass g/ is only | Terminate quality g/ only | Increase day by day quality/g | The % compared with A groups |
| A | 48.2±1.3 | 1979.4±32.1 | 42.8 | 0 |
| B | 48.3±1.7 | 2405.8±62.3 | 52.4 | 22.2 |
| C | 48.2±1.5 | 2450.3±58.9 | 53.4 | 24.8 |
| D | 47.8±2.1 | 2396.4±61.3 | 52.1 | 21.7 |
Each experimental group feed conversion rate contrast of table 5
| Group | Average consumption/chicken quality (g/g) | Feed conversion rate (kg/kg) | Compared with A groups |
| A | 4265.4/1979.4 | 2.21 | 100 |
| B | 4488.5/2405.8 | 1.90 | 86.11(-13.89) |
| C | 4606.1/2450.3 | 1.88 | 85.10(-14.9) |
| D | 4577.8/2396.4 | 1.91 | 86.40(-13.6) |
Table 5 test result indicates that, each experimental group compared with control group feed dress rate significantly improve.
Flavonoids of wheat gem of fermenting (C) and complete fermentation wheat germ powder (D) save feed 13.6-14.9%.Contrast and unite with control group
Meter learns processing P<0.05. statistical procedures P between each experimental group>0.05.
Experiment observes that C and D group animal wastes stickinesses change simultaneously, and diarrhoea size of animal is reduced, animal wastes
It is hardened, says that meaning is larger from environmental angle.
The broiler chicken of table 6 die of illness number and the death rate
| Group | Enter to give up chicken number | Survival chicken number | Die of illness number | Death rate % |
| A | 400 | 368 | 32 | 8.00 |
| B | 400 | 392 | 8 | 2.00 |
| C | 400 | 396 | 5 | 1.25 |
| D | 400 | 395 | 7 | 1.75 |
From the result of table 6, C, the difference that D experimental groups are not statistically significant with the complete feed group containing antibiotic, but
Contrasted with control group, P<0.01.
Influence of the fermentation Flavonoids of wheat gem of table 7 to broiler chicken Immune Organs Index
| Group | Spleen | Thymus gland | The bursa of farbricius |
| A | 0.12±0.01 | 0.12±0.01 | 0.20±0.04 |
| B | 0.14±0.02 | 0.15±0.03 | 0.30±0.05 |
| C | 0.16±0.02 | 0.15±0.01 | 0.31±0.02 |
| D | 0.15±0.01 | 0.14±0.02 | 0.29±0.03 |
The result of table 7 proves fermentation Flavonoids of wheat gem and complete fermentation wheat germ powder can increase immune organ quality, improves tested
Animal immune function.
Embodiment 7, use addition fermentation Flavonoids of wheat gem feed raising piglet experiment
Three groups are randomly divided into from 90 30 ages in days wean piglet, every group of 30 pigs, male and female all have.A groups are experiment contrast
Group, complete feed feed (containing antibiotic)；B groups are fermentation Flavonoids of wheat gem raising group, and 0.05g fermentation Flavonoids of wheat gem/kg are without anti-
The complete feed of raw element；C groups are that 0.35g complete fermentations wheat germ powder/kg is free of antibiotic complete feed.Test in two stages,
First stage observes that 90 ages in days are weaning period piglet raising experiment from 30 ages in days；Second stage is from 90 ages in days to butchering (170
My god).
Data below is recorded in experimentation：Animal starting quantity and final amt；The weight of animals and knot when experiment starts
Body weight during beam；Animal health and clinical change, animal dead situation.
Addition fermentation Flavonoids of wheat gem forage feed weaning period piglet experimental result is referring to table 8.
The addition fermentation Flavonoids of wheat gem forage feed weaning period piglet experimental result of table 8
| Detection project | A groups | B groups | C groups |
| Starting quantity | 30 | 30 | 30 |
| Starting weight (kg) | 12.17 | 12.38 | 12.06 |
| Final amt | 27 (90%) | 30 (100%) | 29 (96.7%) |
| Final body weight (kg) | 28.3 (100%) | 31.8 (112%) | 30.2 (107%) |
| Feed conversion rate | 2.16 | 1.92 | 2.01 |
| Kg/kg, % | 100% | 88.9% (- 11.1) | 94.8% (- 5.2) |
The result of table 8 is visible：60 days wean feeding period piglet survival rates of experimental group B, C group improve 3- compared with added with antibiotic control group
10%；Final increased weight 7-12%, feed conversion rate reduce 5-11%.
Addition fermentation Flavonoids of wheat gem forage feed getting fat phase piglet experimental result is referring to table 9.
The addition fermentation Flavonoids of wheat gem forage feed getting fat phase piglet experimental result of table 9
| Detection project | A groups | B groups | C groups |
| Starting quantity | 27 | 30 | 29 |
| Starting weight (kg) | 28.0 | 31.8 | 30.2 |
| Final amt | 27 (100%) | 30 (100%) | 29 (100%) |
| Final body weight (kg) | 106 (100%) | 114 (108%) | 110 (104%) |
| Feed conversion rate | 3.30 | 2.89 | 3.01 |
| Kg/kg, % | 100% | 87.6% (- 12.4) | 91.2% (- 8.8) |
Getting fat phase pig resistance generally strengthens, and three groups of animals are showed no death.But getting fat pig changes of weight, efficiency of feed utilization
It is completely similar with weaning period experimental result etc. result.As a result confirm：Different phase addition fermentation Flavonoids of wheat gem or complete fermentation wheat
Embryo powder is advantageous to improve pig resistance, reduces disease incident and the death rate, promotes growing for pig, improves food conversion
Rate, effectively reduce feeding cost.
Flavonoids of wheat gem dosage of fermenting is only 1/7th of complete fermentation wheat germ powder, but result is better than complete fermentation wheat germ
Powder, it is also possible to which complete fermentation wheat germ powder contains the event of micro ANFs.Animal feeding result and animal peripheral blood are external
It is the core active principle in wheat germ fermenting mixture that experimental result, which prompts fermentation Flavonoids of wheat gem,.
What is finally illustrated is：Above example is merely to illustrate the implementation process and feature of the present invention, and unrestricted is sent out
Bright technical scheme, although the present invention is described in detail with reference to above-described embodiment, one of ordinary skill in the art should
Work as understanding：The present invention can still be modified or equivalent substitution, without departing from the spirit and scope of the present invention any
Modification or local replacement, all should cover among protection scope of the present invention.
Claims (10)
- The chromocor extract 1. a kind of wheat germ ferments, it extracts from wheat germ fermented supernatant fluid and obtained；Preferably, it is to utilize wheat Embryo fermented supernatant fluid purifies what is obtained through nonpolar or low pole macroporous resin column chromatography.
- The chromocor extract 2. wheat germ according to claim 1 ferments, it has characteristic fingerprint color under the conditions of table 1 of the present invention Spectrum, i.e., seven characteristic peaks in HPLC collection of illustrative plates；OrIt has following characteristics peak under the separation condition of table 1：6.8min；7.25min；8.5min；8.8min；9.5min； 11min；16.5min.
- The chromocor extract 3. wheat germ according to claim 1 ferments, wherein general flavone content is 2.5-5.8mg/g dries Matter.
- The chromocor extract 4. wheat germ according to claim 1 ferments, wherein, wheat germ fermented supernatant fluid is according to following aspect It is prepared：Wheat germ powder, food yeast add water mixed fermentation, and fermentation period 15~72 hours, preferably 24~48 hours are upper after fermentation It is wheat germ fermented supernatant fluid clearly.
- The chromocor extract 5. wheat germ according to claim 4 ferments, wherein, 26~36 DEG C of fermentation temperature, preferably 30 DEG C；Preferably, the weight of wheat germ and yeast ratio is 2:1 to 8:1, more preferably 5:1；Preferably, in fermentation system, the ratio of dry and water is 1:7~1:14, more preferably 1:8~1:10.
- 6. the preparation method of the wheat germ fermentation chromocor extract described in any one of Claims 1 to 5, this method include：The wheat germ fermentation chromocor extract that general flavone obtains is extracted from wheat germ fermented supernatant fluid；Preferably, obtained using wheat germ fermented supernatant fluid through nonpolar or low pole macroporous resin column chromatography purifying general flavone Wheat germ fermentation chromocor extract.
- 7. the wheat germ fermentation chromocor extract described in any one of Claims 1 to 5 is used for as substitute antibiotics feed addictive Prepare the application of production feed or premixed feed；Preferably, Animal diseases are prevented and treated by the use of fermentation Flavonoids of wheat gem substitute antibiotics as feed addictive in the animal feed；Preferably, the animal feed is used to improve letting animals feed yield；OrPreferably, the animal feed is used to improve vaccinated animals antibody titer, extends effective titre in antibody blood, or increase Strong inoculation animal is to disease resistance.
- 8. application according to claim 7, wherein, the animal is ox, horse, sheep, pig, chicken, duck, goose, rabbit or cultivation fish Class.
- 9. application according to claim 7, wherein, in the animal feed, fermentation Flavonoids of wheat gem usage amount is 0.005- 2g/kg feeds, preferably 0.05-0.5g/kg feeds.
- 10. application according to claim 7, wherein, wheat germ fermentation flavones can be used for the preventing and treating of a variety of diseases of animal, such as feel Metachromia inflammation, mycoplasma infection, pig pneumonia, coccidiosis of domestic fowls infection etc.；Wherein, Flavonoids of wheat gem of fermenting can be individually oral or injects infected animal, may be added in feed, and dosage should appropriateness Improve.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610387986 | 2016-06-06 | ||
| CN2016103879861 | 2016-06-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107455551A true CN107455551A (en) | 2017-12-12 |
Family
ID=60545063
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710065496.4A Pending CN107455551A (en) | 2016-06-06 | 2017-02-06 | Wheat germ fermentation chromocor extract and preparation method thereof and the application in animal feeding |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107455551A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108576611A (en) * | 2018-04-03 | 2018-09-28 | 洛阳福切尔生物科技有限公司 | A kind of preparation method of high anti-oxidation activity wheat germ zymotic fluid |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1192099C (en) * | 1997-08-13 | 2005-03-09 | 马特·希德弗吉 | Immunostimulatory and metastais inhibiting fermented vegetal material |
| CN1649508A (en) * | 2002-08-13 | 2005-08-03 | 梅特·希德弗吉 | Use of fermented wheat-germ in feeding and veterinary practice |
| CN102408400A (en) * | 2011-08-17 | 2012-04-11 | 江南大学 | Method for co-production of wheat germ flavone, raffinose and wheat germ concentrated protein by taking degreased wheat germ as raw material |
| CN103224962A (en) * | 2013-05-14 | 2013-07-31 | 聊城九州和谷生物科技有限公司 | Production method of wheat germ fermentation product |
| CN104862238A (en) * | 2015-05-20 | 2015-08-26 | 常熟理工学院 | Saccaromyces cerevisiae and application thereof |
-
2017
- 2017-02-06 CN CN201710065496.4A patent/CN107455551A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1192099C (en) * | 1997-08-13 | 2005-03-09 | 马特·希德弗吉 | Immunostimulatory and metastais inhibiting fermented vegetal material |
| CN1649508A (en) * | 2002-08-13 | 2005-08-03 | 梅特·希德弗吉 | Use of fermented wheat-germ in feeding and veterinary practice |
| CN102408400A (en) * | 2011-08-17 | 2012-04-11 | 江南大学 | Method for co-production of wheat germ flavone, raffinose and wheat germ concentrated protein by taking degreased wheat germ as raw material |
| CN103224962A (en) * | 2013-05-14 | 2013-07-31 | 聊城九州和谷生物科技有限公司 | Production method of wheat germ fermentation product |
| CN104862238A (en) * | 2015-05-20 | 2015-08-26 | 常熟理工学院 | Saccaromyces cerevisiae and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108576611A (en) * | 2018-04-03 | 2018-09-28 | 洛阳福切尔生物科技有限公司 | A kind of preparation method of high anti-oxidation activity wheat germ zymotic fluid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102771625A (en) | Chinese herbal medicine feed supplement and usage method for improving egg quality | |
| CN103005144B (en) | Traditional Chinese medicine microorganism fermentation preparation and preparation method thereof | |
| CN111011643A (en) | Bacterium enzyme fermented traditional Chinese medicine for improving growth performance of broiler chicken and preparation method thereof | |
| CN104054911A (en) | Egg-laying poultry feed additive and egg-laying poultry feed | |
| CN104489420A (en) | Pig feed additive capable of booting immunity and improving meat quality | |
| CN108029885A (en) | Pet care product and preparation method thereof | |
| CN112293603A (en) | Chinese herbal medicine additive for promoting growth of laying hens and improving egg quality and feeding method thereof | |
| CN103141702A (en) | Chinese herb extract feed additive capable of effectively preventing and treating porcine epizootic diarrhea | |
| CN100527986C (en) | Compound feedstuff additive superfines of Chinese traditional medicine, and preparation method | |
| CN105287790B (en) | A kind of fevervine extract and its application | |
| CN104971111A (en) | Traditional Chinese medicine bio-preparation of improving immunity of livestock and poultry, preparation method thereof and feed therewith | |
| KR102012751B1 (en) | Platycodon grandiflorum extract animal feed additive and animal feed | |
| CN103083510A (en) | Chinese medicinal oral liquid for improving poultry immune function and its preparation method | |
| CN1759718A (en) | Green sanitary aquatic products produced by using marine lives as bioreactor and method | |
| CN104222522B (en) | It is a kind of to prevent chicken feed of bamboo silk white diarrhea and preparation method thereof | |
| CN109221606A (en) | A kind of nonreactive shrimp crab mulberry leaf liquid feed additive and the preparation method and application thereof | |
| CN106306431A (en) | Traditional Chinese medicine health-care feed additive facilitating growth promotion of beef cattle and mutton sheep | |
| CN104171481B (en) | A kind of health care bird feed and preparation method thereof | |
| CN104431329A (en) | Green compound feed prepared from periplaneta americana L. waste residue and preparation method of green compound feed | |
| CN107455551A (en) | Wheat germ fermentation chromocor extract and preparation method thereof and the application in animal feeding | |
| CN110074274A (en) | A kind of growth pannage and preparation method thereof | |
| MXPA05001725A (en) | The use of fermented wheat-germ in the feeding and veterinary practice. | |
| CN1685925A (en) | Chinese herbal medicine to fast ferment and culture improved quality of meat cattle | |
| CN107594121A (en) | A kind of fleshy duck fodder | |
| CN103340311B (en) | Anti-stress feed regulating agent for cow |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171212 |