CN107429292B - 底数大于2的指数核酸扩增 - Google Patents

底数大于2的指数核酸扩增 Download PDF

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CN107429292B
CN107429292B CN201580076131.0A CN201580076131A CN107429292B CN 107429292 B CN107429292 B CN 107429292B CN 201580076131 A CN201580076131 A CN 201580076131A CN 107429292 B CN107429292 B CN 107429292B
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primer
sequence
stranded
strand
double
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CN107429292A (zh
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R·希古奇
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Cepheid
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Cepheid
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201580076131.0A 2014-12-15 2015-12-15 底数大于2的指数核酸扩增 Active CN107429292B (zh)

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US201462092102P 2014-12-15 2014-12-15
US62/092,102 2014-12-15
PCT/US2015/065890 WO2016100388A1 (en) 2014-12-15 2015-12-15 Exponential base-greater-than-2 nucleic acid amplification

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EP (1) EP3234188B1 (enExample)
JP (1) JP6804462B2 (enExample)
CN (2) CN107429292B (enExample)
AU (2) AU2015362617B2 (enExample)
CA (1) CA2971006C (enExample)
DK (1) DK3234188T3 (enExample)
ES (1) ES2764975T3 (enExample)
HK (1) HK1247255A1 (enExample)
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JP6804462B2 (ja) 2014-12-15 2020-12-23 セファイド 指数関数の底が２より大きい核酸増幅
EP3287528A1 (de) * 2016-08-25 2018-02-28 AGCT GmbH Verfahren zur amplifikation von nukleinsäuren und kit zu dessen durchführung
CN112074612A (zh) * 2018-02-26 2020-12-11 Agct有限公司 一种具有更高特异性的核酸扩增方法
WO2019162529A1 (de) * 2018-02-26 2019-08-29 Agct Gmbh Verfahren zur primer-verlängerungsreaktion mit verbesserter spezifität
US20210071244A1 (en) * 2018-02-28 2021-03-11 Agct Gmbh Method for the amplification of a nucleic acid with improved specificity
CN113227398B (zh) 2018-10-29 2025-03-14 塞弗德公司 在减少的扩增时间下使用巢式重叠引物的指数基数-3核酸扩增
EP4143335A4 (en) * 2020-04-27 2024-05-22 Cepheid EXPONENTIAL BASE-3 AND LARGER NUCLEIC ACID AMPLIFICATION WITH A CYCLIC PROBE
EP4170046A4 (en) * 2020-06-23 2024-07-24 Heimbiotek Inc. COMPOSITION FOR SEQUENTIAL POLYMERASE CHAIN REACTION AND GENE AMPLIFICATION METHODS THEREOF
CA3206360A1 (en) 2020-12-24 2022-06-30 Integrated Dna Technologies, Inc. Rnase h2 mutants that reduce primer dimers and off-target amplification in rhpcr-based amplicon sequencing with high-fidelity dna polymerases
US20240102085A1 (en) * 2022-02-15 2024-03-28 Cepheid EXPONENTIAL BASE-GREATER-THAN-BASE 2 NUCLEIC ACID AMPLIFICATION USING HIGH- AND LOW-Tm PRIMERS
US20240336961A1 (en) * 2023-02-23 2024-10-10 Cepheid USE OF DESTABILIZING NUCLEOTIDE TRIPHOSPHATES TO REDUCE AMPLICON FOLDING AND Tm
WO2026019604A1 (en) * 2024-07-19 2026-01-22 Cepheid Exponential base-x amplification with hairpin nested primers and universal flanking primers

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HK1247255A1 (zh) 2018-09-21
JP2017537658A (ja) 2017-12-21
WO2016100388A1 (en) 2016-06-23
US11028434B2 (en) 2021-06-08
CN107429292A (zh) 2017-12-01
US20190203266A1 (en) 2019-07-04
US20210395797A1 (en) 2021-12-23
EP3234188B1 (en) 2019-10-23
US20180066304A1 (en) 2018-03-08
US10273534B2 (en) 2019-04-30
CN114369596A (zh) 2022-04-19
AU2022203553A1 (en) 2022-06-16
AU2022203553B2 (en) 2024-08-08
PT3234188T (pt) 2020-01-16
ES2764975T3 (es) 2020-06-05
CN114369596B (zh) 2025-07-08
US11952621B2 (en) 2024-04-09
CA2971006A1 (en) 2016-06-23
JP6804462B2 (ja) 2020-12-23
CA2971006C (en) 2024-05-21
EP3234188A1 (en) 2017-10-25
AU2015362617A1 (en) 2017-06-29
DK3234188T3 (da) 2020-01-20
AU2015362617B2 (en) 2022-03-03

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