CN107417750B - 一种从微生物发酵液中提取环磷酸腺苷的方法 - Google Patents
一种从微生物发酵液中提取环磷酸腺苷的方法 Download PDFInfo
- Publication number
- CN107417750B CN107417750B CN201710343368.1A CN201710343368A CN107417750B CN 107417750 B CN107417750 B CN 107417750B CN 201710343368 A CN201710343368 A CN 201710343368A CN 107417750 B CN107417750 B CN 107417750B
- Authority
- CN
- China
- Prior art keywords
- microbial fermentation
- column
- value
- adenosine monophosphate
- cyclic adenosine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 48
- 230000004151 fermentation Effects 0.000 title claims abstract description 48
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 title claims abstract description 47
- 230000000813 microbial effect Effects 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 37
- 239000007788 liquid Substances 0.000 title claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 70
- 239000000706 filtrate Substances 0.000 claims abstract description 20
- 239000012670 alkaline solution Substances 0.000 claims abstract description 17
- 238000005406 washing Methods 0.000 claims abstract description 16
- 239000003480 eluent Substances 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims abstract description 5
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 42
- 239000000243 solution Substances 0.000 claims description 40
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 238000010828 elution Methods 0.000 claims description 11
- 238000002791 soaking Methods 0.000 claims description 11
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 9
- 230000004913 activation Effects 0.000 claims description 9
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 8
- 235000019441 ethanol Nutrition 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 6
- 230000007935 neutral effect Effects 0.000 claims description 5
- 238000003795 desorption Methods 0.000 claims description 4
- 230000003213 activating effect Effects 0.000 claims description 3
- 239000003513 alkali Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000005554 pickling Methods 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 8
- 239000002253 acid Substances 0.000 abstract description 4
- 239000000047 product Substances 0.000 abstract description 3
- 238000001179 sorption measurement Methods 0.000 description 10
- 230000000694 effects Effects 0.000 description 8
- 238000011049 filling Methods 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 238000000926 separation method Methods 0.000 description 7
- 238000003786 synthesis reaction Methods 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 6
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 3
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 3
- 229960005305 adenosine Drugs 0.000 description 3
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 239000000543 intermediate Substances 0.000 description 3
- 239000007858 starting material Substances 0.000 description 3
- 238000001291 vacuum drying Methods 0.000 description 3
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 229950006790 adenosine phosphate Drugs 0.000 description 2
- 239000003463 adsorbent Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 239000012043 crude product Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- -1 ester compound Chemical class 0.000 description 2
- 230000032050 esterification Effects 0.000 description 2
- 238000005886 esterification reaction Methods 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000007363 ring formation reaction Methods 0.000 description 2
- 238000000967 suction filtration Methods 0.000 description 2
- AEOBEOJCBAYXBA-UHFFFAOYSA-N A2P5P Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1OP(O)(O)=O AEOBEOJCBAYXBA-UHFFFAOYSA-N 0.000 description 1
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 1
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 1
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- 206010002660 Anoxia Diseases 0.000 description 1
- 241000976983 Anoxia Species 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010019280 Heart failures Diseases 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 201000004681 Psoriasis Diseases 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- IVOMOUWHDPKRLL-UHFFFAOYSA-N UNPD107823 Natural products O1C2COP(O)(=O)OC2C(O)C1N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 230000007953 anoxia Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- CJGYSWNGNKCJSB-YVLZZHOMSA-N bucladesine Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](OC(=O)CCC)[C@@H]2N1C(N=CN=C2NC(=O)CCC)=C2N=C1 CJGYSWNGNKCJSB-YVLZZHOMSA-N 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 229940097217 cardiac glycoside Drugs 0.000 description 1
- 239000002368 cardiac glycoside Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000008558 metabolic pathway by substance Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000002107 myocardial effect Effects 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 210000001087 myotubule Anatomy 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- LPMXVESGRSUGHW-HBYQJFLCSA-N ouabain Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@@H]1C[C@@]2(O)CC[C@H]3[C@@]4(O)CC[C@H](C=5COC(=O)C=5)[C@@]4(C)C[C@@H](O)[C@@H]3[C@@]2(CO)[C@H](O)C1 LPMXVESGRSUGHW-HBYQJFLCSA-N 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 238000001935 peptisation Methods 0.000 description 1
- 239000012450 pharmaceutical intermediate Substances 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000035806 respiratory chain Effects 0.000 description 1
- 210000003660 reticulum Anatomy 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 229930002534 steroid glycoside Natural products 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
- C07H19/213—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids containing cyclic phosphate
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
Description
比较项目 | 微生物发酵法 | 化学合成法 | 植物提取法 |
原料 | 次黄嘌呤 | 5’-AMP | 红枣 |
生产成本(元/公斤) | 3600 | 6000 | 40000 |
分离提取工艺 | 简单、高效、安全 | 复杂、污染性大 | 极复杂 |
总收率 | 80%~85% | 40%~50% |
Claims (7)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710343368.1A CN107417750B (zh) | 2017-05-16 | 2017-05-16 | 一种从微生物发酵液中提取环磷酸腺苷的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710343368.1A CN107417750B (zh) | 2017-05-16 | 2017-05-16 | 一种从微生物发酵液中提取环磷酸腺苷的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107417750A CN107417750A (zh) | 2017-12-01 |
CN107417750B true CN107417750B (zh) | 2020-04-21 |
Family
ID=60425057
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710343368.1A Active CN107417750B (zh) | 2017-05-16 | 2017-05-16 | 一种从微生物发酵液中提取环磷酸腺苷的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107417750B (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110240623B (zh) * | 2019-06-18 | 2022-08-12 | 赵琛 | 可降低3',5'-环化腺苷酸中无机盐杂质的结晶方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS52102298A (en) * | 1976-02-25 | 1977-08-27 | Kikkoman Corp | Recovery and purification of 3#,5#-cyclic adenilic acid |
CN1687439A (zh) * | 2005-04-07 | 2005-10-26 | 江苏诚意药业有限公司 | 微生物发酵法生产腺苷的生产工艺 |
CN102260181A (zh) * | 2011-05-13 | 2011-11-30 | 广西大学 | 从甘蔗梢中提取氨基酸的方法 |
CN104151385A (zh) * | 2014-08-15 | 2014-11-19 | 刘琬一 | 一种提取环磷酸腺苷的方法及应用 |
CN104788522A (zh) * | 2015-03-26 | 2015-07-22 | 安徽省皖北药业股份有限公司 | 一种从发酵液中提取环磷酸腺苷的方法 |
-
2017
- 2017-05-16 CN CN201710343368.1A patent/CN107417750B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS52102298A (en) * | 1976-02-25 | 1977-08-27 | Kikkoman Corp | Recovery and purification of 3#,5#-cyclic adenilic acid |
CN1687439A (zh) * | 2005-04-07 | 2005-10-26 | 江苏诚意药业有限公司 | 微生物发酵法生产腺苷的生产工艺 |
CN102260181A (zh) * | 2011-05-13 | 2011-11-30 | 广西大学 | 从甘蔗梢中提取氨基酸的方法 |
CN104151385A (zh) * | 2014-08-15 | 2014-11-19 | 刘琬一 | 一种提取环磷酸腺苷的方法及应用 |
CN104788522A (zh) * | 2015-03-26 | 2015-07-22 | 安徽省皖北药业股份有限公司 | 一种从发酵液中提取环磷酸腺苷的方法 |
Also Published As
Publication number | Publication date |
---|---|
CN107417750A (zh) | 2017-12-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Ni et al. | Column chromatographic extraction and preparation of cordycepin from Cordyceps militaris waster medium | |
CN101157712B (zh) | 一种虫草素的分离纯化方法 | |
CN108026132B (zh) | 一种烟酰胺单核苷酸的纯化方法 | |
CN106008253B (zh) | 一种从米糠中提取高纯度神经酰胺的方法 | |
CN103304613B (zh) | 一种从瓜蒌皮中分离纯化4种核苷类化学成分的方法 | |
CN102718843A (zh) | 一种替考拉宁单组份的制备方法 | |
CN108218948B (zh) | 一种七叶皂苷钠的制备方法 | |
CN113897406B (zh) | 一种从红景天粉末中提取并纯化红景天苷的方法 | |
EP3168225A1 (en) | Fidaxomicin purification method | |
CN110770350A (zh) | 一种利用β-葡萄糖苷酶制备宝藿苷I的方法 | |
CN103012518B (zh) | 一种从杜仲叶中同时提取车叶草苷和绿原酸的生产工艺 | |
CN103304490B (zh) | 一种从瓜蒌皮中分离纯化5种嘌呤及嘧啶碱基的方法 | |
CN107417750B (zh) | 一种从微生物发酵液中提取环磷酸腺苷的方法 | |
CN105949043A (zh) | 一种抗菌消炎化合物的制备方法 | |
CN111056941B (zh) | 一种利用银杏叶提取物层析废液制备高纯度莽草酸的方法 | |
CN104987952B (zh) | 从红景天全草中提取挥发油及红景天甙的方法 | |
CN114807258B (zh) | 一种从海红米糠中提取神经酰胺的方法 | |
WO2012055253A1 (zh) | 一种辅酶q10层析硅胶的再生方法 | |
CN116903688A (zh) | 一种具有urat1抑制活性的密蒙花苷及其制备方法和应用 | |
CN110396058A (zh) | 一种新型骨化二醇(25-羟基维生素d3)分离纯化方法 | |
CN111171096A (zh) | 一种多杀菌素的提取方法 | |
CN109851651A (zh) | 一种大孔吸附树脂从催化液中分离环磷酸腺苷的方法 | |
CN112409426B (zh) | 一种硫酸西索米星的制备方法 | |
CN105111144A (zh) | 一种从荷叶中提取荷叶碱的方法 | |
CN103073624B (zh) | 一种高纯度环孢菌素a衍生物的制备方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240703 Address after: No. 6 Yingjie Avenue, Houhe Professional Park, Houhe Town, Weihui City, Xinxiang City, Henan Province, China 453100 Patentee after: Henan Yingjie Food Co.,Ltd. Country or region after: China Address before: 453003. Eastern section of Hua Hua Avenue, Henan, Xinxiang Patentee before: Henan Institute of Science and Technology Country or region before: China |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240717 Address after: No. 555, South Road, 50 meters east of the intersection of Hualan Avenue and Xinyi Street, Hongmen Town, Hongqi District, Xinxiang City, Henan Province, China 453000 Patentee after: Henan Baoshengkang Biotechnology Co.,Ltd. Country or region after: China Address before: No. 6 Yingjie Avenue, Houhe Professional Park, Houhe Town, Weihui City, Xinxiang City, Henan Province, China 453100 Patentee before: Henan Yingjie Food Co.,Ltd. Country or region before: China |