CN107412103A - A kind of antioxidant and its application - Google Patents
A kind of antioxidant and its application Download PDFInfo
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- CN107412103A CN107412103A CN201710704742.6A CN201710704742A CN107412103A CN 107412103 A CN107412103 A CN 107412103A CN 201710704742 A CN201710704742 A CN 201710704742A CN 107412103 A CN107412103 A CN 107412103A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
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- Dermatology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of antioxidant and its application, the antioxidant is made up of the component of following parts by weight：0.2 2.0 parts of wax gourd seed extract, 0.1 1.8 parts of olive polyphenol, 0.2 2.0 parts of balsam pear flavones.It is applied in emulsion, by removing free radical, anti peroxidation of lipid, improving activities of antioxidant enzymes and other effects, reaches the skin that compacts, the effect of anti-aging.
Description
Technical field
The present invention relates to cosmetic field, and in particular to a kind of antioxidant and its application.
Background technology
The mood of the aging course of human skin and the degree of aging and residing environment, age and people are closely related.With
The growth at age, the change and stimulation of outside climatic environment, causes the sebaceous glands of skin and sweat gland secretion abnormal, the skin of human body
Fat, moisture secretion can be gradually decreased, and the gradual aging of skin of people, sebum secretion is reduced, and the ability of vitrification moisture can decline,
So that skin becomes more and more dry, the cuticula of epiderm skin thickens, and produces skin keratin, dry and cracked, skin surface just
It can become coarse.Oxidation is the biggest threat of skin aging, and diet is unhealthy, and Exposure to Sunlight, pressure, environmental pollution etc. can allow skin
Free radical spreads unchecked, so as to produce the oxidative phenomenas such as complexion dimness, water shortage.
The content of the invention
Based on this, it is an object of the invention to provide a kind of antioxidant, and it, which has, removes the work(such as free radical, anti-oxidant
Effect, and it is gently non-stimulated to skin.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of antioxidant is made up of the component of following parts by weight：Wax gourd seed extract 0.2-2.0 parts, olive polyphenol
0.1-1.8 parts, balsam pear flavones 0.2-2.0 parts.
Preferably, described antioxidant is made up of the component of following parts by weight：Wax gourd seed extract 0.3-1.6 parts, oil
Olive polyphenol 0.2-1.2 parts, balsam pear flavones 0.3-1.5 parts.
Optimal, described antioxidant is made up of following parts by weight of component：Wax gourd seed extract 0.5-1.2 parts, oily olive
Olive polyphenol 0.4-1.0 parts, balsam pear flavones 0.6-1.0 parts.
Further, the preparation method of described wax gourd seed extract is：Clean wax gourd seed is weighed, according to solid-liquid ratio 1:
10 add distilled water, and after soaking 1h, 1-2h is heated to reflux at 90-95 DEG C, cool down, and filter, and filter residue repeats the above steps repetition
Extraction twice, merging filtrate, takes filtrate to be steamed in Rotary Evaporators near and done, produce wax gourd seed extract.
Further, the preparation method of described olive polyphenol is as follows：Olive is gathered, 10 times of parts by weight of addition
50% ethanol carries out refluxing extraction 3 times, each 2h, merges extract solution, filtering, is freezed after the concentration of gained filtrate rotary evaporation dry
It is dry, obtain olive polyphenol crude extract；Olive crude extract is adsorbed using macroreticular resin, parses, purify, it is more to obtain olive
Phenol.
Further, the preparation method of described balsam pear flavones is as follows：According to weight ratio (1：6) by balsam pear and 65-75%
Ethanol decocts 2-3 times in 85-90 DEG C of constant temperature water bath, each 1-2h, filters to get filtrate；Using ethanol in Rotary Evaporators recovered liquid
Solution, obtain concentrated extract；Put in separatory funnel and extract 2-3 times, obtain balsam pear flavones.
The present invention also provides a kind of application of antioxidant in cosmetics are prepared, and the cosmetics are emulsion.
Further, described emulsion includes the component of following parts by weight：Above-mentioned antioxidant 1.5-3.2 parts, glycerine
It is 8.1-12 parts, butanediol 4.0-8.7 parts, isopropyl palmitate 2.5-3.4 parts, caprylic/capric triglyceride 1.0-5.5 parts, hard
Resin acid 3.1-5.0 parts, dimethyl silicone polymer 0.6-1.0 parts, cyclomethicone 0.6-1.8 parts, Gantrez AN-119
0.1-0.5 parts, aloe extract 0.001-0.003 parts, jojoba seed oil 0.01-0.02 parts, Sodium Hyaluronate 0.01-0.05
Part, xanthans 0.01-0.03 parts, Phenoxyethanol 0.1-0.5 parts, Sensiva SC50 0.05-0.2 parts, deionized water surplus.
Several components in the antioxidant used in the present invention are introduced below：
Wax gourd seed be it is double be dicotyledon medicine cucurbitaceous plant wax gourd seed, alias pumpkin seeds《This warp》, melon seeds, melon
Valve《Golden tall building outline》, Semen Benincasae《Do not record》, melon rhinoceros《Jingchusuishiji》, national the greater part is produced.With moistening lung for removing phlegm, disappear carbuncle
Li Shui, for treating phlegm-heat cough, lung carbuncle, acute appendicitis, gonorrhoea, oedema, tinea pedis, hemorrhoid, nasal surface wine drum etc..Wax gourd seed water extract
With lipid peroxidation outside excellent scavenging hydroxyl, superoxide radical effect and antibody.
Olive is Oleaceae Olea plant, is a kind of evergreen dungarunga and fruit with seeds, main in olive growing leaves
Active component is that Polyphenols, the flavonoids such as oleuropein and flavonoids mainly have galuteolin, apiin, Quercetin, rutin, mountain
Naphthol etc..Studying the single aldehydes matters such as the oleuropein for showing extractive of olive growing leaves and its containing, tyrosol has notable removing
DPPH free radicals, the ability of anti peroxidation of lipid.Olive polyphenol Scavenging ability has synergy；Olive polyphenol
Slow down lipid peroxidation process, and improve activities of antioxidant enzymes.
Balsam pear also known as bright and beautiful lichee, insane grape, balsam pear, it is the battalion such as cucurbitaceous plant, the charantin being rich in pulp and seed
Forming point has good hypoglycemic effect, have it is hypoglycemic, anti-oxidant, improve immunity, anti-bacteria and anti-virus, immunological regulation,
A variety of pharmacological activity such as antiallergy, analgesia, hypotensive.The researchs such as Cao second place find that Momordica charantial can significantly improve mouse aging
The activity of GSH-Px, SOD in liver, brain tissue and serum, the MDA reduced in mouse aging liver, brain tissue and serum contain
Amount.Show that Momordica charantial has the function that anti-aging.Balsam pear flavone compound can substantially suppress the oxidation of lard, and add
Amount is more, and oxidation resistance is stronger.The increase of balsam pear flavones content, the number increase of its active hydrogen, so as to there is more active hydrogens
Atom is combined with peroxide caused by grease Auto-oxidation reaction, interrupt chain reaction, so as to prevent oxidizing process continue into
OK.
Compared with prior art, it is the device have the advantages that as follows：The present invention is using wax gourd seed extract, olive
Compounding use, three kinds of compositions act synergistically by a certain percentage for polyphenol, balsam pear flavones, pass through effect of scavenging radical and anti-grease matter mistake
Oxidation, improves the activity of antioxidase, while improves immunity of organisms, and is combined by hydrogen atom with peroxide, in
Chain rupture lock reactor, the continuation of oxidizing process is prevented, so as to play oxidation resistant effect.Wherein, wax gourd seed extract can be removed
Hydroxy radical, superoxide radical effect and the outer lipid peroxidation of antibody；Removing can be acted synergistically freely by adding olive polyphenol
Base, slow down lipid peroxidation process, and improve activities of antioxidant enzymes；With with balsam pear flavones, increase human body in active hydrogen number,
So as to there are more reactive hydrogen atoms to be combined with peroxide caused by grease Auto-oxidation reaction, chain reaction is interrupted, so as to
Oxidizing process is prevented to continue.Product of the present invention also has lock containing aloe extract, jojoba seed oil, Sodium Hyaluronate etc.
Water moisturizing, the component of moisturizing effect, make skin maintain quantitative moisture, booster action is played to anti-oxidant, anti-aging.
Embodiment
Below in conjunction with specific embodiment, the present invention is further elaborated：
All the components appeared in text are INCI (International Nomenclature Cosmetic
Ingredient), i.e., international cosmetic raw material is obviously said to its defined title.
The emulsion embodiment 1~5 of the present invention is formulated, and is shown in Table 1.
Table 1
The preparation method of plant composition：
1. the preparation method of wax gourd seed extract is：
Clean wax gourd seed is weighed, according to solid-liquid ratio 1:10 add distilled water, after soaking 1h, are heated back at 90~95 DEG C
1~2h is flowed, is cooled down, is filtered, filter residue repeats the above steps repetition extraction twice, merging filtrate, takes filtrate to be steamed in Rotary Evaporators
Done near, produce wax gourd seed extract.
2. the preparation method of olive polyphenol is as follows：
Olive is gathered, 50% ethanol for adding 10 times of parts by weight carries out refluxing extraction 3 times, each 2h, merges extract solution,
Filtering, it is freeze-dried after the concentration of gained filtrate rotary evaporation, obtains olive polyphenol crude extract；Olive crude extract is used into macropore
Resin is adsorbed, parsed, purified, and obtains olive polyphenol.
3. the preparation method of balsam pear flavones is as follows：
According to weight ratio (1:6) balsam pear and 65~75% ethanol are decocted 2~3 times in 85~90 DEG C of constant temperature water baths, every time 1
~2h, filters to get filtrate；Using ethanol solution in Rotary Evaporators recovered liquid, concentrated extract is obtained；Put and 2 are extracted in separatory funnel
~3 times, obtain balsam pear flavones.
Comparative example 1：
Wax gourd seed extract is not added in difference with embodiment 3 only, remaining each material addition, and conditions and technology of preparation is equal
Do not change.
Comparative example 2：
Olive polyphenol is not added in difference with embodiment 3 only, and remaining each material addition, conditions and technology of preparation is not
Change.
Comparative example 3：
Balsam pear flavones is not added in difference with embodiment 3 only, and remaining each material addition, conditions and technology of preparation is not sent out
It is raw to change.
Comparative example 4：
Compared with Example 3, wax gourd seed extract, olive polyphenol, balsam pear flavones do not add, remaining each material addition
Amount, conditions and technology of preparation do not change still.
Anti-oxidation efficacy is verified：
Tris-HCL cushioning liquid 4.5mL and deionized water 3.2mL are taken, 20min is incubated in 25 DEG C of water-baths, is stood after taking-up
Add in 25 DEG C of preheated 3mmol/L pyrogallol 0.3mL, pour into cuvette after shaking up rapidly, every 30s under 325mm
Absorbance is surveyed, calculates the increase of range of linearity internal absorbance.
As stated above, aliquots of deionized water is replaced with the embodiment of above-mentioned different formulations or comparative example sample respectively, together
Sample surveys prominent absorbance every 30s.
Inhibiting rate is calculated with following formula, inhibiting rate is bigger, and to represent oxidation resistance bigger：
Inhibiting rate (%)=(△ A1/△t-△A2/△t)/(△A1/ △ t) x100%
Wherein：△A1/△t：Mouse thymus cells reaction rate；
△A2/ △ t add the reaction rate of pyrogallol after antioxidant.
Using mouse thymus cells reaction assay free radical scavenger to the clear of its caused ultra-oxygen anion free radical
Except effect.Autoxidation can occur in the basic conditions for pyrogallol, discharge oxonium ion free radical, and have light at 325nm
Absorb；Different inhibitory action can be produced to oxonium ion free radical by adding a certain amount of sample solution, so as to cause absorbance value
Change.
The result of the test that each component suppresses mouse thymus cells experiment is shown in Table 2.
Table 2
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | |
| Inhibiting rate (%) | 82.6 | 77.2 | 86.6 | 80.6 | 81.9 |
| Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Deionized water | |
| Inhibiting rate (%) | 52.6 | 51.7 | 55.1 | 35.6 | 8.3 |
, it is known that suppress mouse thymus cells ability strengthens with the inoxidizability of sample.It can be seen that the implementation of equivalent
Example sample rejection ability is even more the inoxidizability of remote super deionized water apparently higher than comparative example.Illustrate that the present invention is anti-oxidant
Each composition of agent has inoxidizability, and can act synergistically, and further enhances the inoxidizability of whole product.
Above example is merely to illustrate embodiment of the present invention, does not have any formal limit to the present invention
System；It is any to be engaged in professional and technical personnel, all have the ability using the technology contents having revealed that, some are made to it and changes or repaiies
Decorations, turn into the equivalent embodiment of equivalent variations.According to the technology of the present invention essence, simply modification, equivalent variations are carried out to the present invention
With modification, belong in the range of technical solution of the present invention.
Claims (9)
1. a kind of antioxidant, it is characterised in that described antioxidant is made up of the component of following parts by weight：Wax gourd seed carries
Take thing 0.2-2.0 parts, olive polyphenol 0.1-1.8 parts, balsam pear flavones 0.2-2.0 parts.
2. a kind of antioxidant according to claim 1, it is characterised in that described antioxidant is by following parts by weight
Component forms：Wax gourd seed extract 0.3-1.6 parts, olive polyphenol 0.2-1.2 parts, balsam pear flavones 0.3-1.5 parts.
3. a kind of antioxidant according to claim 2, it is characterised in that described antioxidant is by following parts by weight group
It is grouped into：Wax gourd seed extract 0.5-1.2 parts, olive polyphenol 0.4-1.0 parts, balsam pear flavones 0.6-1.0 parts.
4. according to a kind of antioxidant described in claim any one of 1-3, it is characterised in that described wax gourd seed extract
Preparation method is：Clean wax gourd seed is weighed, according to solid-liquid ratio 1:10 add distilled water, after soaking 1h, are heated at 90-95 DEG C
Flow back 1-2h, cools down, and filters, and filter residue repeats the above steps repetition extraction twice, merging filtrate, takes filtrate to be steamed in Rotary Evaporators
Done near, produce wax gourd seed extract.
5. according to a kind of antioxidant described in claim any one of 1-3, it is characterised in that the system of described olive polyphenol
Preparation Method is as follows：Olive is gathered, 50% ethanol for adding 10 times of parts by weight carries out refluxing extraction 3 times, each 2h, merges extraction
Liquid, filtering, it is freeze-dried after the concentration of gained filtrate rotary evaporation, obtains olive polyphenol crude extract；Olive crude extract is used
Macroreticular resin is adsorbed, parsed, purified, and obtains olive polyphenol.
6. according to a kind of antioxidant described in claim any one of 1-3, it is characterised in that the preparation of described balsam pear flavones
Method is as follows：According to weight ratio (1：6) balsam pear and 65-75% ethanol are decocted 2-3 times in 85-90 DEG C of constant temperature water bath, each 1-
2h, filter to get filtrate；Using ethanol solution in Rotary Evaporators recovered liquid, concentrated extract is obtained；Put and 2-3 is extracted in separatory funnel
It is secondary, obtain balsam pear flavones.
A kind of 7. application of the antioxidant according to claim any one of 1-6 in cosmetics are prepared.
8. application according to claim 7, it is characterised in that described cosmetics are emulsion.
9. a kind of emulsion with antioxidation, it is characterised in that described emulsion includes the component of following parts by weight：Such as power
Profit requires antioxidant 1.5-3.2 parts described in 1, glycerine 8.1-12 parts, butanediol 4.0-8.7 parts, isopropyl palmitate 2.5-
3.4 parts, caprylic/capric triglyceride 1.0-5.5 parts, stearic acid 3.1-5.0 parts, dimethyl silicone polymer 0.6-1.0 parts, ring gather
Dimethyl siloxane 0.6-1.8 parts, Gantrez AN-119 0.1-0.5 parts, aloe extract 0.001-0.003 parts, Jojoba seed
Oily 0.01-0.02 parts, Sodium Hyaluronate 0.01-0.05 parts, xanthans 0.01-0.03 parts, Phenoxyethanol 0.1-0.5 parts, ethyl
Hexyl glycerine 0.05-0.2 parts, deionized water surplus.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108158877A (en) * | 2017-12-25 | 2018-06-15 | 马南行 | A kind of oxidation resistant olive composition and preparation method thereof |
| CN111437231A (en) * | 2020-04-18 | 2020-07-24 | 北温草生物科技(上海)有限公司 | Chinese herbal medicine composition for resisting wrinkles and resisting aging |
| CN113841690A (en) * | 2021-11-17 | 2021-12-28 | 李广超 | Protective agent for CAR-T cell cryopreservation and cryopreservation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101744756A (en) * | 2008-12-17 | 2010-06-23 | 马桂珍 | Chinese medicinal multiple-effect skin beautifying cream and preparation method thereof |
| CN101849899A (en) * | 2009-12-24 | 2010-10-06 | 上海原尚草化妆品有限公司 | Chinese medicinal compound extract and cosmetics comprising same as active ingredient |
| CN105362676A (en) * | 2014-08-22 | 2016-03-02 | 香港浸会大学 | Preparation method and application of traditional Chinese medicine composition having multiple effects for skin care |
-
2017
- 2017-08-16 CN CN201710704742.6A patent/CN107412103A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101744756A (en) * | 2008-12-17 | 2010-06-23 | 马桂珍 | Chinese medicinal multiple-effect skin beautifying cream and preparation method thereof |
| CN101849899A (en) * | 2009-12-24 | 2010-10-06 | 上海原尚草化妆品有限公司 | Chinese medicinal compound extract and cosmetics comprising same as active ingredient |
| CN105362676A (en) * | 2014-08-22 | 2016-03-02 | 香港浸会大学 | Preparation method and application of traditional Chinese medicine composition having multiple effects for skin care |
Non-Patent Citations (1)
| Title |
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| 王建新: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108158877A (en) * | 2017-12-25 | 2018-06-15 | 马南行 | A kind of oxidation resistant olive composition and preparation method thereof |
| CN111437231A (en) * | 2020-04-18 | 2020-07-24 | 北温草生物科技(上海)有限公司 | Chinese herbal medicine composition for resisting wrinkles and resisting aging |
| CN113841690A (en) * | 2021-11-17 | 2021-12-28 | 李广超 | Protective agent for CAR-T cell cryopreservation and cryopreservation method |
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