KR102363846B1 - Cosmetic composition containing natural extracts complex - Google Patents

Abstract

The present invention relates to a cosmetic composition containing as an active ingredient a mixed extract of Laminaria digitata, Fucus vesiculosus, Camellia sinensis leaf, Diospyros kaki leaf and Oryza sativa bran. More specifically, the present invention relates to a cosmetic composition, which contains, as an active ingredient a mixed extract of Laminaria digitata, Fucus vesiculosus, Camellia sinensis leaf, Diospyros kaki leaf and Oryza sativa bran, alleviates skin inflammation and irritation caused by fine dust, and is highly effective in removing fine dust and heavy metals.

Description

Cosmetic composition containing natural extracts complex

The present invention relates to a cosmetic composition containing a natural mixed extract, and specifically, a cosmetic composition having excellent skin protection activity from fine dust by containing kelp, bladder rag algae, green tea leaf, persimmon leaf and rice bran mixed extract as an active ingredient. it's about

The skin is a part of the body that is directly exposed to the external environment, and it not only acts as a protective film to protect important organs of our body, but also regulates water evaporation and protects the body from external infections. However, excessive UV rays and stress caused by a polluted environment cause skin irritation and eventually lead to skin aging.

In recent years, heavy metals and fine dust caused by rapid industrialization have been cited as major causes of skin irritation and aging. Heavy metals refer to heavy metal elements with a specific gravity of 4 or more, such as lead, mercury, cadmium, zinc, and nickel. . In addition, it has been reported that the skin may cause a chemical reaction in the air to additionally generate harmful substances such as nitrogen oxide (NO), which may cause skin inflammation, atopy, and troubles.

Fine dust is a general term for dust with a particle size of 10 μm or less, and is abbreviated as PM 10 (Particulate Matter 10). Such fine dust is produced by photochemical reaction in the atmosphere between toxic substances or heavy metals from automobile smoke and factory chimneys, and it is known that toxic substances such as sulfate and nitrate and heavy metals are included. These fine dust particles are small and can go deep into the skin pores and cause skin troubles, and worsen various symptoms such as dry skin, irritant dermatitis, allergic dermatitis, acne, and pigmentation.

Therefore, the occurrence of dermatitis induced by fine dust is expected to increase, and the need for materials that can alleviate skin inflammatory reactions induced by heavy metals and fine dust is increasing.

Accordingly, the present inventors have been searching for natural materials that have the activity to protect the skin from fine dust, and the kelp, bladder rag algae, green tea leaf, persimmon leaf and rice bran mixed extract relieves skin inflammation and irritation caused by fine dust, The present invention was completed by confirming that the effect of removing fine dust and heavy metals was excellent.

(0001) Republic of Korea Patent Publication No. 10-2019-0018843 (2019.02.26) (0002) Republic of Korea Patent Publication No. 10-2017-0012989 (2017.02.06) (0003) Republic of Korea Patent No. 10-1786861 (2017.10.11)

The present invention provides a cosmetic composition containing kelp, bladder rag algae, green tea leaf, persimmon leaf and rice bran mixed extract as an active ingredient to relieve skin inflammation and irritation caused by fine dust, and to provide a cosmetic composition excellent in removing fine dust and heavy metals aim to

According to the present invention to achieve the above object, there is provided a cosmetic composition containing kelp, bladder rag algae, green tea leaf, persimmon leaf, and a mixed extract of rice bran as an active ingredient.

Preferably, the mixed extract is kelp, bladder rack algae, green tea, persimmon leaf and rice bran in a weight ratio of 1-3:1-2:1-4:2-4:0.5-1, more preferably 2:1, respectively. It is extracted by mixing in a weight ratio of ~2:1~2:3~4:1, most preferably 2:1:1:4:1.

The mixed extract as an active ingredient is contained in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition.

The cosmetic composition is characterized in that it is for relieving skin inflammation caused by fine dust, for alleviating skin irritation due to fine dust, or for removing fine dust and heavy metals.

The kelp, bladder rag algae, green tea leaf, persimmon leaf and rice bran mixed extract of the present invention is useful as a cosmetic that protects the skin from fine dust because it is excellent in suppressing the inflammatory reaction caused by fine dust, alleviating irritation, and removing fine dust and heavy metals. can be used

Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition containing kelp, bladder rag algae, green tea leaf, persimmon leaf and rice bran mixed extract as an active ingredient.

Kelp is a brown algae widely consumed in coastal areas of Korea, China and Japan. About 20 species of kelp are grown on the Pacific coast, and main species include kelp kelp (L. japonica), kombu Okhotsk (L.ochotensis), kelp kelp (L. religiosa), and the like. It has been eaten in East Asia such as Korea, Japan, and China since ancient times. Kelp contains a large amount of water-soluble polysaccharides such as alginic acid and fucoidan.

Bladderwrack algae are brown seaweeds of the family Fucaceae, distributed in the North Sea, Baltic Sea, Atlantic Ocean, and Pacific Ocean. It contains a large amount of iodine and mucilage, and contains minerals and vitamins such as aluminum, barium, boron, calcium, cobald, copper, potassium, iron iodine, lithium, and magnesium.

Green tea is produced by inactivating the oxidative enzyme present in the tea leaves by using the buds or leaves, and has been consumed as a favorite tea. The pharmacological actions of antioxidant, anti-inflammatory, antibacterial, anti-viral, anti-aging, etc. by various components contained in green tea are known.

Persimmon leaf contains vitamins A and C, and contains rutin, which has hemostasis and blood pressure lowering effects. Due to the astringent action of the tannins contained in persimmon leaves, it contracts the tissues of the mucous membrane in the body, stopping diarrhea and alleviating the symptoms of stomach ulcers. In addition, the astringent action of tannic acid shows an effect on burns.

Rice bran refers to the pulverized product of pericarp, seed hide and aloe layer obtained when brown rice is polished into white rice. Rice bran contains a large amount of vitamin A, B vitamins such as thiamine, pyridoxine and niacin, and minerals such as calcium, zinc, and iron. In addition, rice bran contains a large amount of lysine, an essential amino acid, and unsaturated fatty acids such as oleic acid, linolenic acid, and linoleic acid, so it has excellent nutritional value.

According to the present invention, there is provided a cosmetic composition containing the mixed extract of kelp, bladder rag algae, green tea leaf, persimmon leaf and rice bran as an active ingredient.

The mixed extract is a hot water extract, water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, the group consisting of petroleum ether and diethyl ether It is a solvent extract with at least one solvent selected from

Extraction may be performed by a conventional method. According to one embodiment of the present invention, the kelp, bladder rack algae, green tea leaf, persimmon leaf, and rice bran are immersed at a stable temperature or extracted under reflux by heating in an extractor equipped with a cooling condenser, then filtered and concentrated under reduced pressure to obtain the concentrate under reduced pressure. It is prepared by freeze-drying. In addition, it may include a low-temperature aging or high-temperature aging process after performing the extraction process.

At this time, the mixed extract is kelp, bladder rack algae, green tea, persimmon leaf and rice bran in a weight ratio of 1-3:1-2:1-4:2-4:0.5-1, more preferably 2:1- It is prepared by extraction by mixing in a weight ratio of 2:1 to 2:3 to 4:1, most preferably in a weight ratio of 2:1:1:4:1.

The kelp, bladder rag algae, green tea leaf, persimmon leaf and rice bran mixed extract of the present invention prepared in this way has superior inflammatory reaction suppression effect and irritation relief effect due to fine dust compared to each component due to its synergistic action, It is advantageous for skin protection from dust, and it was confirmed that the cleansing formulation to which the mixed extract was added had excellent ability to remove fine dust and heavy metals.

The kelp, bladder rag algae, green tea leaf, persimmon leaf and rice bran mixed extract may be added in an amount of 0.01 to 20 wt % based on the total weight of the cosmetic composition. When it is added in an amount of less than 0.01% by weight, the effect is weak, and when it exceeds 20% by weight, the increase in the effect is decreased compared to the increase in the content, which is rather inefficient.

The cosmetic composition of the present invention can be prepared in the form of general emulsified formulations and solubilized formulations. Cosmetics in emulsified formulations include nutritional lotions, creams, and essences, and solubilized cosmetics include softening lotions. In addition, by containing a dermatologically acceptable medium and base in addition to cosmetics, it can be prepared in the form of an adjuvant that can be applied topically and systemically commonly used in the field of dermatology.

In addition, the formulation of the cosmetic composition in the present invention is not limited, but skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, essence , pack, soap, shampoo, treatment, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder, and eye shadow.

[Example]

Hereinafter, the present invention will be described in more detail based on the following examples and test examples. However, the following examples are only for illustrating the present invention, and the present invention is not limited by the following examples.

Examples 1-8: Preparation of mixed extracts

After mixing dried kelp, bladder rag algae, green tea leaf, persimmon leaf, and rice bran in the ratio shown in Table 1 below, put it in 70% hydrated ethanol equivalent to 10 times the dry weight, and heat to 80 ° C in the extractor with no cooling condenser. time was extracted. This was concentrated at a temperature of 50 to 60 ℃ using a reduced pressure concentrator, and then freeze-dried with a freeze dryer to obtain an extract powder.

weight ratio Example One 2 3 4 5 6 7 8 Kelp One 2 3 2 2 2 3 2 Bladder Wack Algee One One One One One 2 2 2 green tea leaves 2 3 4 2 One 2 One 4 persimmon leaves 2 2 2 3 4 4 3 2 rice bran 0.5 0.5 One One One One One One

Comparative Example 1: Preparation of kelp extract

The dried kelp was put in 70% aqueous ethanol corresponding to 10 times the dry weight, and extracted for 8 hours at 80°C in an extractor with a different cooling condenser. This was concentrated at a temperature of 50 to 60 ℃ using a reduced pressure concentrator, and then freeze-dried with a freeze dryer to obtain an extract powder.

Comparative Example 2: Preparation of bladder rag algae extract

The dried bladder rack AL paper was placed in 70% aqueous ethanol equivalent to 10 times the dry weight and extracted for 8 hours at 80°C in an extractor with a different cooling condenser. This was concentrated at a temperature of 50 to 60° C. using a vacuum concentrator, and then freeze-dried using a freeze dryer to obtain an extract powder.

Comparative Example 3: Preparation of green tea leaf extract

The dried green tea leaves were put in 70% aqueous ethanol corresponding to 10 times the dry weight and extracted for 8 hours at 80°C in an extractor with a cooling condenser. This was concentrated at a temperature of 50 to 60 ℃ using a reduced pressure concentrator, and then freeze-dried with a freeze dryer to obtain an extract powder.

Comparative Example 4: Preparation of persimmon leaf extract

The dried persimmon leaves were put in 70% aqueous ethanol corresponding to 10 times the dry weight, and extracted for 8 hours at 80° C. in an extractor using a cooling condenser. This was concentrated at a temperature of 50 to 60° C. using a vacuum concentrator, and then freeze-dried using a freeze dryer to obtain an extract powder.

Comparative Example 5: Preparation of rice bran extract

The dried rice bran was placed in 70% aqueous ethanol equivalent to 10 times the dry weight, and extracted for 8 hours at 80° C. in an extractor using a cooling condenser. This was concentrated at a temperature of 50 to 60 ℃ using a reduced pressure concentrator, and then freeze-dried with a freeze dryer to obtain an extract powder.

Test Example 1: Inhibitory effect of apoptosis induced by fine dust

The effect of inhibiting apoptosis caused by fine dust was confirmed. Human-derived fibroblasts were cultured in a 96-well plate at a concentration of 1×10 ⁶ cells/well, 0.2 ml of IMDM medium containing 10% FBS was supplied, and then cultured in a CO ₂ incubator for 24 hours. Here, 25 ppm of fine dust was treated with the sample, and after incubation for 24 hours, 10 μL of MTT solution (5 mg/mL) was added. After 4 hours, the medium was discarded, DMSO was added to dissolve formazan, and absorbance was measured at 570 nm using an ELISA reader. Cell viability (%) was measured by the following formula, and the results are shown in Table 2 below.

Cell viability (%) = [(St-Bo/(Bt-Bo)] × 100

Bo: Absorbance at 570 nm of wells in which only the cell culture medium was subjected to color development

Bt: Absorbance at 570 nm of wells subjected to color reaction without sample treatment

St: Absorbance at 570 nm of wells subjected to sample treatment and color reaction

division Cell viability (%) 100ppm 500ppm 1000ppm Comparative Example 1 56.9 73.1 78.9 Comparative Example 2 57.4 71.8 77.6 Comparative Example 3 57.0 72.5 79.0 Comparative Example 4 64.5 74.4 80.7 Comparative Example 5 53.2 67.8 73.7 Example 1 69.5 78.3 82.4 Example 2 68.6 75.8 81.3 Example 3 74.6 82.0 85.1 Example 4 76.2 82.8 86.5 Example 5 78.0 83.6 89.3 Example 6 75.8 82.0 87.1 Example 7 73.7 79.0 83.9 Example 8 70.9 77.1 82.0

As can be seen from Table 2, it was confirmed that the cell death inhibitory effect caused by fine dust in the sample to which the mixed extract of Example was applied was superior to the cell death inhibitory effect of the single extract sample of Comparative Example.

Test Example 2: Reduction of COX-2 and PGE-2 expression by fine dust

In order to examine the effect on the cytokine production of Human Epidermal Keratinocyte (HaCaT), 100 ul of 5×10 ⁵ cells/ml cells were put into a 96 well plate, incubated in a CO ₂ incubator for 48 hours, and then the medium was discarded, After washing the surface with PBS solution, it was replaced with a medium not added with FBS, starvated for 18 hours, and cultured for 24 hours. After incubation, the cells were stimulated by treatment with PMs (25ppm), a stimulatory factor, and then treated with 0.1% of each sample of Comparative Examples and Examples. After culturing for 24 hours, the cells were cultured for 24 hours using RT-PCR. Cells were examined for mRNA expression levels using RT-PCR.

Using the mRNA expression level increased by each of the stimulatory factors as a control, the expression reduction rate was calculated by the following formula, and the results are shown in Table 3.

mRNA expression reduction rate (%) = [1- (sample mRNA expression level / control group mRNA expression level)] × 100

division COX-2 expression reduction rate PGE2 expression reduction rate Preparation Example 1 19.7 15.2 Preparation 2 18.3 16.4 Preparation 3 20.8 17.6 Preparation 4 19.6 16.8 Preparation 5 14.1 9.9 Example 1 21.0 15.7 Example 2 22.3 17.0 Example 3 23.5 18.4 Example 4 26.8 19.1 Example 5 28.0 19.7 Example 6 27.4 18.8 Example 7 24.4 18.3 Example 8 23.9 17.7 Dexamethasone 25.2 18.6

As can be seen in Table 3 above, the mixed extract of the Example of the present invention significantly reduced the expression of the inflammatory factors COX-2 and PGE-2 induced by fine dust compared to the single extract of the Comparative Example, Example 4 to 6 showed a more excellent effect.

Test Example 3: Effect of reducing NF-kB and TNF-a expression by fine dust

In order to examine the effect on the cytokine production of Human Epidermal Keratinocyte (HaCaT), 100 ul of 5×10 ⁵ cells/ml cells were put into a 96 well plate, incubated in a CO ₂ incubator for 48 hours, and then the medium was discarded, After washing the surface with PBS solution, it was replaced with a medium not added with FBS, starvated for 18 hours, and cultured for 24 hours. After incubation, the cells were stimulated by treatment with PMs (25ppm), a stimulatory factor, and then treated with 0.1% of each sample of Comparative Examples and Examples. After culturing for 24 hours, the cells were cultured for 24 hours using RT-PCR. Cells were examined for mRNA expression levels using RT-PCR.

Using the mRNA expression level increased by each of the stimulatory factors as a control, the expression reduction rate was calculated by the following formula, and the results are shown in Table 4.

mRNA expression reduction rate (%) = [1- (sample mRNA expression level / control group mRNA expression level)] × 100

division NF-kB expression reduction rate TNF-a expression reduction rate Preparation Example 1 17.0 13.7 Preparation 2 16.8 13.0 Preparation 3 15.4 15.3 Preparation 4 18.2 14.7 Preparation 5 9.3 6.8 Example 1 21.2 17.6 Example 2 23.7 18.8 Example 3 25.9 21.8 Example 4 30.7 23.2 Example 5 32.3 24.4 Example 6 31.5 23.9 Example 7 27.4 22.2 Example 8 26.0 20.9 Dexamethasone 29.3 22.6

As can be seen in Table 4, the mixed extract of the example of the present invention showed the expression of the inflammatory factors NF-kB and TNF-a induced by fine dust compared to the single extract of the comparative example. It was greatly reduced, and more excellent effects were exhibited in Examples 4 to 6.

Test Example 4: Test for inhibition of IL-1a and 1b expression by stimulation

In order to examine the effect on the cytokine production of Human Epidermal Keratinocyte (HaCaT), 100 ul of 5×10 ⁵ cells/ml cells were put into a 96 well plate, incubated in a CO ₂ incubator for 48 hours, and then the medium was discarded, After washing the surface with PBS solution, it was replaced with a medium not added with FBS, starvated for 18 hours, and cultured for 24 hours. After culturing, the cells were stimulated by treatment with PMs (25ppm), a stimulatory factor, treated with 0.1% of each sample of Preparation Examples and Examples, and cultured for 24 hours. Then, the cells were cultured for 24 hours using RT-PCR. Cells were examined for mRNA expression levels using RT-PCR.

Using the mRNA expression level increased by each of the stimuli as a control, the expression reduction rate was calculated by the following formula, and the results are shown in Table 5.

mRNA expression reduction rate (%) = [1- (sample mRNA expression level / control group mRNA expression level)] × 100

division NF-kB expression reduction rate TNF-a expression reduction rate Preparation Example 1 23.7 27.3 Preparation 2 26.0 24.2 Preparation 3 20.3 25.0 Preparation 4 27.5 28.4 Preparation 5 10.4 11.6 Example 1 28.8 32.0 Example 2 27.0 29.3 Example 3 30.0 31.1 Example 4 33.2 33.5 Example 5 34.4 34.8 Example 6 31.5 32.9 Example 7 29.6 30.2 Example 8 28.2 29.7 Dexamethasone 30.8 32.4

As can be seen in Table 5 above, the mixed extract of the example of the present invention significantly reduced the expression of the inflammatory factors IL-1a and 1b induced by fine dust compared to the single extract of the comparative example, and the best effect in Example 5 was shown.

Example 9: Preparation of Cream Containing Mixed Extracts

A cream containing the mixed extract of Example 5 was prepared according to a conventional method with the composition and content shown in Table 6 below.

ingredient content (wt%) Control Example 1 Example 9 Mixed extract (Example 5) - 2.0 lipophilic monostearate glycerin 2.0 2.0 stearyl alcohol 2.2 2.2 stearic acid 1.5 1.5 beeswax 1.0 1.0 Polysorbate 60 1.5 1.5 Sorbitan Stearate 0.6 0.6 purified vegetable oil 1.0 1.0 squalane 3.0 3.0 mineral oil 5.0 5.0 trioctanoin 5.0 5.0 dimethicone 1.0 1.0 Sodium Magnesium Silicate 0.1 0.1 glycerin 5.0 5.0 betaine 3.0 3.0 triethanolamine 1.0 1.0 Sodium Hyaluronate 4.0 4.0 preservatives, fragrances, colors a very small amount a very small amount Purified water remaining amount remaining amount Sum 100 100

Test Example 5: Confirmation of skin redness alleviation effect

In order to confirm the skin redness alleviation effect, the formulation of Example 9 and Control Example 1 was used to evaluate the soothing effect of skin redness relief in 20 women who met the test subject selection criteria. Subjects were prohibited from using functional care products that could affect the experimental results.

Before the start of the experiment, the redness of the skin to be measured was photographed, and after washing (2 times/day) every morning and evening for 2 weeks, comparative evaluation was performed. For evaluation, a spectrophotometer (Spectrophotomotor CR-2600D, Konica Minolta, Japan) was used.

As a value to measure the soothing effect of redness relief, the a* value indicating the redness of the skin was used for the analysis, and as the measured value decreases compared to before use, it means that there is a calming effect by alleviating redness. The improvement evaluation of the sedative effect by redness relief was measured using the following formula, and then shown in Table 7.

division Initial (D0) mean 2 weeks (D14) average Improvement (%) Control Example 1 12.42 12.03 3.14 Example 9 12.26 11.17 8.90

As a result of analyzing the improvement of the soothing effect by alleviating redness using a spectrophotometer, the cosmetic composition of Example 9 containing the mixed extract of the present invention had a* value indicating redness of the skin compared to Control Example 1. It was confirmed that the improvement rate for alleviation was excellent.

Example 10: Preparation of Cleansing Foam Containing Mixed Extracts

A cleansing foam containing the mixed extract of Example 5 was prepared according to a conventional method with the composition and content of Table 8 below.

ingredient content (wt%) Control Example 2 Example 10 Mixed extract (Example 5) - 10.0 stearic acid 8.0 8.0 myristic acid 12.0 12.0 vitamin E acetate 1.0 1.0 glycerin 10.0 10.0 propylene glycol 15.0 15.0 Cocamide Profile Betaine 7.0 7.0 Cocamide DEA 7.0 7.0 Ethylenediaminetetraacetic acid 0.2 0.2 potassium hydroxide 5.0 5.0 fragrance, preservative a very small amount a very small amount Purified water remaining amount remaining amount Sum 100 100

Test Example 6: Skin heavy metal removal effect

Two samples of pig skin (1 cm * 1 cm) were immersed in 100 ppm lead solution and cadmium solution for 90 minutes, respectively, dried at room temperature, and then washed by applying the cleansing foam of Example 10 and Control Example 2, followed by inductively coupled plasma The removal rate was measured through the residual amount of heavy metals in the skin through mass spectrometry (ICP-MS). The removal rate was calculated by dividing the concentration of the sample after removal of heavy metals by the concentration of the sample before removal, and the results are shown in Table 9 below.

division Control Example 2 Example 10 Cadmium (Cd) 29.77 62.32 Lead (Pb) 35.80 69.05

As can be seen in Table 9, when the cleansing foam containing the mixed extract of Example 5 was used, the heavy metal removal effect was excellent.

Claims (6)

A mixed extract of kelp, bladder rack algae, green tea leaf, persimmon leaf and rice bran extracted by mixing kelp, bladder rag algae, green tea leaf, persimmon leaf and rice bran in a weight ratio of 2:1 to 2:1 to 2:3 to 4:1, respectively. A cosmetic composition containing as an active ingredient. delete The cosmetic composition according to claim 1, wherein the mixed extract as an active ingredient is contained in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the cosmetic composition is for alleviating skin inflammation caused by fine dust. The cosmetic composition according to claim 1, wherein the cosmetic composition is for alleviating skin irritation caused by fine dust. The cosmetic composition according to claim 1, wherein the cosmetic composition is for removing fine dust and heavy metals.