CN105362676A - Preparation method and application of traditional Chinese medicine composition having multiple effects for skin care - Google Patents
Preparation method and application of traditional Chinese medicine composition having multiple effects for skin care Download PDFInfo
- Publication number
- CN105362676A CN105362676A CN201410417672.2A CN201410417672A CN105362676A CN 105362676 A CN105362676 A CN 105362676A CN 201410417672 A CN201410417672 A CN 201410417672A CN 105362676 A CN105362676 A CN 105362676A
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- extract
- skin care
- skin
- medicine composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Landscapes
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a preparation method of a traditional Chinese medicine composition for skin care and an extractive thereof, the composition having multiple effects on skin-whitening, antioxidation, ultraviolet radiation absorption and so on. The composition comprises, by weight, 10 parts of rhizoma bletillae, 10 parts of pericarpium granati, 10 parts of semen benincasae, and 10 parts of rhizoma typhonii. The invention also provides the application of the traditional Chinese medicine composition in skin care. The traditional Chinese medicine composition is natural, green, healthy and safe. The extractive of the composition can inhibit activity of tyrosinase and melanogenesis effectively, has multiple effects on scavenging free radicals, absorbing ultraviolet radiation and so on, is safe to skin and free of stimulation, can be used as a multifunctional additive of skin care products for preparing various skin care products, and has wide application value.
Description
Technical field
The present invention relates to a kind of Skin-care Chinese medicine compositions, specifically a kind of Chinese medicinal skin care compositions extract simultaneously with multi-efficiencies such as whitening, antioxidation and absorption ultraviolet and the Chinese medicinal skin care preparation prepared by this skincare composition extract.
Background technology
Each women wishes that oneself own water tenderly moistens, peach and cream, smoothly to compact and whippy skin.Accomplish to appear brilliance from skin deep layer, will skin cell vitality be excited, and clear away the melanin inside skin, open skin inherent honorable.The complete concept of skin nursing comprise sun-proof, promote skin metabolism, cutin maintenance, antioxidation, moisturizing, calmly shine the omnibearing maintenance such as skin afterwards.
For the feature of skin cell, the main path of skin-whitening and skin nursing comprises the following aspects: (1), by suppressing melanocyte propagation in skin, reduces the quantity that melanocyte generates; (2) catalytic activity of the key enzyme-tryrosinase generated by check melanin, is reduced melanic biosynthesis, reaches the object of whitening; (3) by sun-proof, prevent sunlight middle-ultraviolet lamp to the injury (tanned and sunburn) of skin, slow down skin aging; (4) be the oxygen-derived free radicals removed by use antioxidant in skin, slow down melanin and generate and slow down skin aging.
Based on the tight demand of skin nursing, there is countless skin-protection product on the market.Whitening agent in current domestic and international most of cosmetics is all active by restraint of tyrosinase, reaches the effect of whitening to reduce melanic generation.Although some non-naturals and natural whitening agent are found and apply, seldom have both safety in them, the product that effect is good again, as hydroquinone is very large to the zest of skin, European Union forbade adding hydroquinone in cosmetics in 2002; Kojic acid is easy to change and also have certain zest to skin, and life-time service has potential carcinogenic risk; Though arbutin to a certain extent restraint of tyrosinase is active, there is certain photosensitivity simultaneously, easily burden is caused to skin, accelerate skin aging, etc.In addition, some conventional natural anti-oxidation additive, due to poor stability, adds in cosmetics, and long-term placement was easily lost efficacy.If water miscible vitamin C is to light, thermally labile, and difficult Transdermal absorption; Fat-soluble vitamin A is to photo-labile, and potential skin irritation, etc.
Along with people increasing skin health attention rate, exploitation has safety and stability, successful and the high natural skin care product additive of cost performance, has become one of main direction of studying of current medicine and cosmetic industry, has had extraordinary development prospect.Natural materials is applied to cosmetics, not only can reaches the multi-efficiency such as skin protection and skin treating, there is diverse in function, widely applicable, security performance advantages of higher simultaneously, thus day by day come into one's own.
Herein based on China's Traditional Chinese Medicine theory, choose the four taste Chinese medicines such as Pseudobulbus Bletillae (Rhizoma Bletillae), Pericarpium Granati, Semen Benincasae and Rhizoma Typhonii and carry out scientific composition, choose wherein the most effective active site, demonstrate its depression effect that tyrosinase activity in mouse black-in lymphoma B16 cell and melanin are generated, the depression effect to tyrosinase activity in human melanin HEMn cell, the Scavenging activity to multiple free radical and absorb the multiple efficacies such as ultraviolet, have developed a a kind of Chinese medicine composition extract simultaneously with complex functions such as whitening, antioxidation and absorption ultraviolet.Meanwhile, zooperal result also proves, the invention provides Chinese medicine composition extract non-stimulated to skin, safety is high.
Summary of the invention
The object of the present invention is to provide a kind of Chinese medicinal skin care compositions of multi-efficiency.
One object of the present invention is the preparation and the purification process that provide a kind of above-mentioned Chinese medicine composition active component.
Another object of the present invention is to provide the skin care compositions utilizing above-mentioned Chinese medicine extract to prepare.
Chinese medicine composition of the present invention comprise Pseudobulbus Bletillae (Rhizoma Bletillae) (
bletillastriata(Thunb.) dry tuber of Reichb.f.), Pericarpium Granati (
punicagranatuml. dry peel), Semen Benincasae (
benincasahispida(Thunb.) dry seed of Cogn.) and Rhizoma Typhonii (
typhoniumgiganteumengl. dry tuber) combination of four Chinese medicine thing, there is skin moistening and brighten, improve the multi-efficiencies such as dull-looking skin.Effect brief introduction of four Chinese medicine thing is as follows:
Pseudobulbus Bletillae (Rhizoma Bletillae): Pseudobulbus Bletillae (Rhizoma Bletillae) cold nature is bitter in the mouth, sweet, puckery.Return lung, liver, stomach warp.There is the effect such as astringing to arrest bleeding, detumescence and promoting granulation.
Pericarpium Granati: Pericarpium Granati is warm in nature is sour in the mouth, puckery.Return large intestine channel.There is relieving diarrhea with astringents, hemostasis, the effects such as anthelmintic.
Semen Benincasae: Semen Benincasae cold nature, sweet in the mouth.Return lung, large intestine channel.There is the effects such as removing heat from the lung and dissipating phlegm, eliminating carbuncle evacuation of pus, dampness removing.
Rhizoma Typhonii: Rhizoma Typhonii is warm in nature, acrid in the mouth.Return stomach, Liver Channel.There is the expectorant that dispels the wind, arresting convulsion jerked, detoxicating and resolving stagnation of pathogens, the effect such as pain relieving.
In the present invention, weight portion refers to any unit of weight, such as gram (g), kilogram (kg) etc.
In the present invention, bulking value refers to the volume of solvent and the ratio (v/w) of medical material weight, such as milliliter (mL)/gram (g), liter (L)/kilogram (kg) etc.
The invention provides a kind of Chinese medicinal skin care compositions with multi-efficiencies such as whitening, antioxidation and absorption ultraviolet, this Chinese medicinal skin care compositions is made up of Pseudobulbus Bletillae (Rhizoma Bletillae), Pericarpium Granati, Semen Benincasae and Rhizoma Typhonii.
In certain embodiments, the present composition is made up of the crude drug of following weight: Pseudobulbus Bletillae (Rhizoma Bletillae) 10 weight portion, Pericarpium Granati 10 weight portion, Semen Benincasae 10 weight portion and Rhizoma Typhonii 10 weight portion.
In certain embodiments, the weight proportion of described Pseudobulbus Bletillae (Rhizoma Bletillae), Pericarpium Granati, Semen Benincasae and Rhizoma Typhonii is 1:1:1:1.
The invention provides described Chinese medicine extract or described Chinese medicine composition is preparing the application in external-use skin care preparation.
In certain embodiments, external-use skin care preparation of the present invention is emulsifiable paste, ointment, facial cream, essence or cleansing milk.
The invention provides a kind of skin care product additive with multi-efficiencies such as whitening, antioxidation and absorption ultraviolet, it comprises Chinese medicine extract of the present invention or Chinese medicine composition of the present invention and the acceptable adjuvant of skin care field.
The invention provides a kind of method preparing Chinese medicine composition extract, it comprises:
A) described Pseudobulbus Bletillae (Rhizoma Bletillae), Pericarpium Granati, Semen Benincasae and Rhizoma Typhonii is taken according to 1:1:1:1 weight proportion;
B) by after the water of medicinal at least 5 times of bulking values or alcohol solution dipping, heating and refluxing extraction;
C) medicinal residues use the water of at least 5 times of bulking values or alcoholic solution heating and refluxing extraction 1 time again, merge extracted twice liquid;
D) after being cooled to room temperature, with filter paper or frit, get filtrate and be concentrated into suitable volume;
E) concentrated solution is added to after static adsorption reaches balance in macroporous resin column, is eluted to eluent with deionized water colourless;
F) with ethanol elution, the concentration of ethanol is 20% ~ 95%;
G) collect ethanol elution, be evaporated to after removing ethanol completely, with appropriate water dissolution extract, through lyophilization, obtain Chinese medicine extract powder provided by the invention.
In the methods of the invention, step (b) and the water of (c) or the volume of alcoholic solution can be specially 5-20 times of bulking value, such as 5-15 doubly, 5-10 doubly or 5-8 doubly.Soak time is with reference to this area routine operation.Such as can soak 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 10 hours or 15,20 hours.
In the method for the invention, those of filter paper or filter or well known and conventional use in step (d), the aperture of such as filter paper or filter can in 2-10 micrometer range, such as 3-8 micron, 3-5 micron.
In the method for the invention, the concentration of ethanol used in step (f) can 20-95%, 30-80%, 40-70%, and 50-60%, is specifically as follows 20%, 50%, 80% or 95%.
In the methods of the invention, described step (d) Chinese medicine extraction concentrated solution, need through macroporous resin adsorption purification.
In the methods of the invention, resin absorption thing in described step (e), need use ethanol elution, the concentration of ethanol is 20% ~ 95%, obtains extract powder finally by lyophilization.
The Chinese medicine extract that the inventive method prepares can be applicable to prepare external-use skin care preparation.
External-use skin care preparation of the present invention comprises emulsifiable paste, ointment, facial cream, essence, cleansing milk, etc.
The invention provides a kind of skin care product additive with multi-efficiencies such as whitening, antioxidation and absorption ultraviolet, it comprises Chinese medicine extract and the acceptable adjuvant of skin care field that the inventive method prepares.
The invention provides Chinese medicine composition, through the Chinese medicine extract extracted, purification obtains, there is good skin-care effect.Whole process preparation method is easy, utilizes the method easily can prepare skin care formulation.Product safety of the present invention is reliable, effect significantly, easily absorbs.Chinese medicine extract itself has check melanin generation, antioxidation, slow down aging, alleviates the effects such as ultraviolet radiation stimulation, therefore both effectiveness plant additive can be it can be used as to add in cosmetics, especially have in the cosmetics of whitening skin-active senile-resistant efficacy in preparation, there is extensive use.
Accompanying drawing explanation
Fig. 1 is that Chinese medicine composition extract provided by the invention (embodiment 7 prepares) is to tyrosinase activity inhibition in B16 melanoma cells.
▼ ▼ p<0.01 has significant differences relative to blank; *
p<0.05 has significant difference relative to α-melanocyte stimulating hormone regulatory hormone contrast; *
p<0.01 has significant differences relative to α-melanocyte stimulating hormone regulatory hormone contrast.
Fig. 2 is that Chinese medicine composition extract provided by the invention (embodiment 7 prepares) is on the impact of melanin content in B16 melanoma cells.
▼ ▼ p<0.01 has significant differences relative to blank; *
p<0.05 has significant difference relative to α-melanocyte stimulating hormone regulatory hormone contrast; *
p<0.01 has significant differences relative to α-melanocyte stimulating hormone regulatory hormone contrast.
Fig. 3 is that Chinese medicine composition extract provided by the invention (embodiment 7 prepares) is to the inhibition of tyrosinase activity in human melanocytes HEMn.*
p<0.05 has significant difference relative to blank; *
p<0.01 has significant differences relative to blank.
Fig. 4 is the active effect that Chinese medicine composition extract provided by the invention (embodiment 7 prepares) removes DPPH free radical.
Fig. 5 is that Chinese medicine composition extract provided by the invention (embodiment 7 prepares) removes ABTS
●+the active effect of free radical.
Fig. 6 is Chinese medicine composition extract provided by the invention (embodiment 7 prepares) ultraviolet absorption effect.Indicate Chinese medicine composition extract and to the ultraviolet of 200 ~ 400nm wave band, there is good Absorption when 25 μ g/mL and 10 μ g/mL, even if Chinese medicine composition extract (10 μ g/mL) under lower concentration still has well absorb ultraviolet effect.
Fig. 7 is that Chinese medicine composition extract provided by the invention (embodiment 7 prepares) is to guinea pig skin zest evaluation map.
Detailed description of the invention
The present embodiment medical material used is all bought from Chinese herbalist clinic of Hong Kong Baptist University and is obtained, and quality meets Pharmacopoeia of People's Republic of China version standard in 2010.
The required solution preparation of experiment:
A: phosphate buffer: take 9.55g phosphate buffer powder (DPBS), is dissolved in 950mL distilled water, is adjusted to pH=6.8, then adds distilled water to 1,000mL with the hydrochloric acid of 10N;
B:0.2%L-tyrosine solution: accurately take 0.2gL-tyrosine, dissolves by the phosphate buffered solution (PBS) of pH=6.8 and is settled to 100mL;
C:1%TritonX-100 phosphate buffered solution (1%TritonX-100PBS): the TritonX-100 accurately pipetting 1mL, dissolves by the phosphate buffered solution (PBS) of pH=6.8 and is settled to 100mL.
Embodiment:
B16 cell DMEM culture fluid is cultivated; Human melanoma cell (HEMn) is cultivated with M254 culture fluid.
(1) mensuration (96 well plate method) of tyrosinase activity in B16 cell
The cell of exponential phase is selected to make single cell suspension, with every hole 3 × 10
3cell/1mL is inoculated in 96 well culture plates, in 37 DEG C, volume fraction is 5%CO
2incubator in overnight incubation; After removing culture fluid, every hole adds the culture fluid 200 μ L containing variable concentrations extract respectively, simultaneously not add the culture fluid of extract as blank, take Arbutin as positive control; After dosing, by cell 37 DEG C, volume fraction is 5%CO
2incubator in cultivate.After 48 hours, incline culture fluid, washes 2 times with normal saline; Add the 1%TritonX-100 phosphate buffer solution (pH=6.8) of 75 μ L, after-20 DEG C of multigelation cracking; Add 75 μ L0.2%L-tyrosine solutions, at 37 DEG C, lucifuge hatches 2 hours, under 475nm wavelength, measure absorbance; By the relative tyrosinase activity of following formulae discovery:
Relative tyrosinase activity (%)=drug treating group/blank group × 100%
(2) mensuration (24 well plate method) of tyrosinase activity in B16 cell
The cell of exponential phase is selected to make single cell suspension, with every hole 4 × 10
4cell/1mL is inoculated in 24 well culture plates, in 37 DEG C, volume fraction is 5%CO
2incubator in overnight incubation; After removing culture fluid, every hole adds culture fluid (α-melanocyte stimulating hormone regulatory hormone/α-MSH containing the 10nM) 1mL containing variable concentrations extract respectively, simultaneously with blank culture fluid and containing 10nM α-MSH blank culture fluid in contrast, with the α-MSH of the H-89(of 5 μMs containing 10nM) for positive control; After dosing, by cell 37 DEG C, volume fraction is 5%CO
2incubator in cultivate.After 48 hours, incline culture fluid, washes 2 times with normal saline; Add the 1%TritonX-100 phosphate buffer solution (pH=6.8) of 150 μ L, after-20 DEG C of multigelation cracking, be transferred in the centrifuge tube of 1.5mL, with the speed of 14,000rpm at 4 DEG C centrifugal 10 minutes; The protein content in supernatant is measured with Coomassie Brilliant Blue; The supernatant simultaneously drawing 50 μ L is in 96 orifice plates, and add 50 μ L0.2%L-tyrosine solutions, at 37 DEG C, lucifuge hatches 2 hours, measures absorbance, correct with protein content under 475nm wavelength; By the relative tyrosinase activity of following formulae discovery:
Relative tyrosinase activity (%)=drug treating group/blank group × 100%
(3) mensuration (24 well plate method) of B16 cell amount in B16 cell
The cell of exponential phase is selected to make single cell suspension, with every hole 4 × 10
4cell/1mL is inoculated in 24 well culture plates, in 37 DEG C, volume fraction is 5%CO
2incubator in overnight incubation; After removing culture fluid, every hole adds culture fluid (α-melanocyte stimulating hormone regulatory hormone/α-MSH containing the 10nM) 1mL containing variable concentrations extract respectively, simultaneously with blank culture fluid and containing 10nM α-MSH blank culture fluid in contrast, with the α-MSH of the H-89(of 5 μMs containing 10nM) for positive control; After dosing, by cell 37 DEG C, volume fraction is 5%CO
2incubator in cultivate.After 48 hours, incline culture fluid, washes 2 times with normal saline; Add the 1%TritonX-100 phosphate buffer solution (pH=6.8) of 150 μ L, after-20 DEG C of freezing-thawing and crackings, be transferred in the centrifuge tube of 1.5mL, with the speed of 14,000rpm at 4 DEG C centrifugal 10 minutes; The protein content in supernatant is measured with Coomassie Brilliant Blue; After removing supernatant, add the sodium hydroxide solution of 100 μ L1N, under 405nm wavelength, measure absorbance in 80 DEG C of water bath heat preservations after 2 hours; With melanin standard product drawing standard curve, calculate melanic content in cell, correct with protein content; By following formulae discovery relative melanin synthetic quantity:
Relative melanin synthetic quantity (%)=drug treating group/blank group × 100%
(4) mensuration (24 well plate method) of the middle tyrosinase activity of human melanocytes (HEMn)
The cell of exponential phase is selected to make single cell suspension, with every hole 5 × 10
4cell/1mL is inoculated in 24 well culture plates, in 37 DEG C, volume fraction is 5%CO
2incubator in overnight incubation; After removing culture fluid, every hole adds the culture fluid 1mL containing variable concentrations extract respectively, simultaneously not add the culture fluid of extract in contrast, take Arbutin as positive control; After dosing, by cell 37 DEG C, volume fraction is 5%CO
2incubator in cultivate.After 48 hours, incline culture fluid, washes 2 times with normal saline; Add the 1%TritonX-100 phosphate buffer solution (pH=6.8) of 150 μ L, after-20 DEG C of multigelation cracking, be transferred in the centrifuge tube of 1.5mL, with the speed of 14,000rpm at 4 DEG C centrifugal 10 minutes; The protein content in supernatant is measured with Coomassie Brilliant Blue; The supernatant simultaneously drawing 50 μ L is in 96 orifice plates, and add 50 μ L0.2%L-tyrosine solutions, at 37 DEG C, lucifuge hatches 2 hours, measures absorbance, correct with protein content under 475nm wavelength; By the relative tyrosinase activity of following formulae discovery:
Relative tyrosinase activity (%)=drug treating group/blank group × 100%
The preparation of embodiment 1 Chinese medicine extract of the present invention
(1) each raw material of Chinese medicine is taken according to following compositions:
Pseudobulbus Bletillae (Rhizoma Bletillae) 5g, Pericarpium Granati 5g, Semen Benincasae 5g, Rhizoma Typhonii 5g
(2) by the water soaking of medicinal 10 times of bulking values, soak time is 1 hour, heating and refluxing extraction 2 hours;
(3) and then by the water heating and refluxing extraction 2 hours of 10 times of bulking values, extracted twice liquid is merged;
(4), after being cooled to room temperature, with filter paper or the frit of 3 ~ 5 μm, getting filtrate and be concentrated into suitable volume;
(5) concentrated solution is carried out lyophilization, obtain Chinese medicine extract provided by the invention.
The preparation of embodiment 2 Chinese medicine extract of the present invention
(1) each raw material of Chinese medicine is taken according to following compositions:
Pseudobulbus Bletillae (Rhizoma Bletillae) 5g, Pericarpium Granati 5g, Semen Benincasae 5g, Rhizoma Typhonii 5g
(2) by 20% soak with ethanol of medicinal 10 times of bulking values, soak time is 1 hour, heating and refluxing extraction 2 hours;
(3) and then with 20% alcohol heating reflux of 10 times of bulking values extract 2 hours, merge extracted twice liquid;
(4) after being cooled to room temperature, with filter paper or the frit of 3 ~ 5 μm;
(5) getting filtrate reduced in volume to removing after ethanol completely, with appropriate water dissolution extract, through lyophilization, obtaining Chinese medicine extract provided by the invention.
The preparation of embodiment 3 Chinese medicine extract of the present invention
(1) each raw material of Chinese medicine is taken according to following compositions:
Pseudobulbus Bletillae (Rhizoma Bletillae) 5g, Pericarpium Granati 5g, Semen Benincasae 5g, Rhizoma Typhonii 5g
(2) by 50% soak with ethanol of medicinal 10 times of bulking values, soak time is 1 hour, heating and refluxing extraction 2 hours;
(3) and then with 50% alcohol heating reflux of 10 times of bulking values extract 2 hours, merge extracted twice liquid;
(4) after being cooled to room temperature, with filter paper or the frit of 3 ~ 5 μm;
(5) getting filtrate reduced in volume to removing after ethanol completely, with appropriate water dissolution extract, through lyophilization, obtaining Chinese medicine extract provided by the invention.
The preparation of embodiment 4 Chinese medicine extract of the present invention
(1) each raw material of Chinese medicine is taken according to following compositions:
Pseudobulbus Bletillae (Rhizoma Bletillae) 5g, Pericarpium Granati 5g, Semen Benincasae 5g, Rhizoma Typhonii 5g
(2) by 80% soak with ethanol of medicinal 10 times of bulking values, soak time is 1 hour, heating and refluxing extraction 2 hours;
(3) and then with 80% alcohol heating reflux of 10 times of bulking values extract 2 hours, merge extracted twice liquid;
(4) after being cooled to room temperature, with filter paper or the frit of 3 ~ 5 μm;
(5) getting filtrate reduced in volume to removing after ethanol completely, with appropriate water dissolution extract, through lyophilization, obtaining Chinese medicine extract provided by the invention.
The preparation of embodiment 5 Chinese medicine extract of the present invention
(1) each raw material of Chinese medicine is taken according to following compositions:
Pseudobulbus Bletillae (Rhizoma Bletillae) 5g, Pericarpium Granati 5g, Semen Benincasae 5g, Rhizoma Typhonii 5g
(2) by 95% soak with ethanol of medicinal 10 times of bulking values, soak time is 1 hour, heating and refluxing extraction 2 hours;
(3) and then with 95% alcohol heating reflux of 10 times of bulking values extract 2 hours, merge extracted twice liquid;
(4) after being cooled to room temperature, with filter paper or the frit of 3 ~ 5 μm;
(5) getting filtrate reduced in volume to removing after ethanol completely, with appropriate water dissolution extract, through lyophilization, obtaining Chinese medicine extract provided by the invention.
The preparation of embodiment 6 Chinese medicine extract of the present invention
(1) each raw material of Chinese medicine is taken according to following compositions:
Pseudobulbus Bletillae (Rhizoma Bletillae) 5g, Pericarpium Granati 5g, Semen Benincasae 5g, Rhizoma Typhonii 5g
(2) by 80% soak with ethanol of medicinal 10 times of bulking values, soak time is 1 hour, heating and refluxing extraction 2 hours;
(3) and then with 80% alcohol heating reflux of 10 times of bulking values extract 2 hours, merge extracted twice liquid;
(4), after being cooled to room temperature, with filter paper or the frit of 3 ~ 5 μm, getting filtrate and be concentrated into suitable volume;
(5) concentrated solution to be added in macroporous resin column static adsorption 4 hours, first to use the water elution of 10 times of column volumes colourless to eluent;
(6) ethanol elution of 20% is then used;
(7) collect ethanol elution, be evaporated to after removing ethanol completely, with appropriate water dissolution extract, through lyophilization, obtain Chinese medicine extract provided by the invention.
The preparation of embodiment 7 Chinese medicine extract of the present invention
(1) each raw material of Chinese medicine is taken according to following compositions:
Pseudobulbus Bletillae (Rhizoma Bletillae) 5g, Pericarpium Granati 5g, Semen Benincasae 5g, Rhizoma Typhonii 5g
(2) by 80% soak with ethanol of medicinal 10 times of bulking values, soak time is 1 hour, heating and refluxing extraction 2 hours;
(3) and then with 80% alcohol heating reflux of 10 times of bulking values extract 2 hours, merge extracted twice liquid;
(4), after being cooled to room temperature, with filter paper or the frit of 3 ~ 5 μm, getting filtrate and be concentrated into suitable volume;
(5) concentrated solution to be added in macroporous resin column static adsorption 4 hours, first to use the water elution of 10 times of column volumes colourless to eluent;
(6) ethanol elution of 50% is then used;
(7) collect ethanol elution, be evaporated to after removing ethanol completely, with appropriate water dissolution extract, through lyophilization, obtain Chinese medicine extract provided by the invention.
The preparation of embodiment 8 Chinese medicine extract of the present invention
(1) each raw material of Chinese medicine is taken according to following compositions:
Pseudobulbus Bletillae (Rhizoma Bletillae) 5g, Pericarpium Granati 5g, Semen Benincasae 5g, Rhizoma Typhonii 5g
(2) by 80% soak with ethanol of medicinal 10 times of bulking values, soak time is 1 hour, heating and refluxing extraction 2 hours;
(3) and then with 80% alcohol heating reflux of 10 times of bulking values extract 2 hours, merge extracted twice liquid;
(4), after being cooled to room temperature, with filter paper or the frit of 3 ~ 5 μm, getting filtrate and be concentrated into suitable volume;
(5) concentrated solution to be added in macroporous resin column static adsorption 4 hours, first to use the water elution of 10 times of column volumes colourless to eluent;
(6) ethanol elution of 80% is then used;
(7) collect ethanol elution, be evaporated to after removing ethanol completely, with appropriate water dissolution extract, through lyophilization, obtain Chinese medicine extract provided by the invention.
The preparation of embodiment 9 Chinese medicine extract of the present invention
(1) each raw material of Chinese medicine is taken according to following compositions:
Pseudobulbus Bletillae (Rhizoma Bletillae) 5g, Pericarpium Granati 5g, Semen Benincasae 5g, Rhizoma Typhonii 5g
(2) by 80% soak with ethanol of medicinal 10 times of bulking values, soak time is 1 hour, heating and refluxing extraction 2 hours;
(3) and then with 80% alcohol heating reflux of 10 times of bulking values extract 2 hours, merge extracted twice liquid;
(4), after being cooled to room temperature, with filter paper or the frit of 3 ~ 5 μm, getting filtrate and be concentrated into suitable volume;
(5) concentrated solution to be added in macroporous resin column static adsorption 4 hours, first to use the water elution of 10 times of column volumes colourless to eluent;
(6) ethanol elution of 95% is then used;
(7) collect ethanol elution, be evaporated to after removing ethanol completely, with appropriate water dissolution extract, through lyophilization, obtain Chinese medicine extract provided by the invention.
The preparation of embodiment 10 skin protection emulsifiable paste of the present invention
Raw material and proportioning (mass percent):
1, raw material and consumption:
Chinese medicine extract 10 ~ 30g
Oil phase: emulsifing wax 90g
White beeswax 150g
Liquid paraffin 60g
Aqueous phase: phenoxyethanol 10g
Deionized water 700g
2, preparation method:
(1) each raw material is taken by above-mentioned component and consumption;
(2) A phase (oil phase) and B phase (aqueous phase) being heated to 80 DEG C of stirrings respectively makes material dissolution complete;
(3) A is added in emulsifying pot stirs, stir speed (S.S.) is 100 revs/min, be added in emulsifying pot by B while stirring, the speed added is as the criterion with the whole emulsifying of B phase raw material in emulsifying pot, continues to stir homogenizing 10 minutes after B phase raw material all adds, stir speed (S.S.) is 100 revs/min, be heated to 80 DEG C of insulations, start cooling after 20 minutes, add Chinese medicine extraction raw material when temperature drops to 60 DEG C, cooling is continued, the homogenizing discharging in 1 ~ 2 minute when temperature is at 20 DEG C after stirring.
The preparation of embodiment 11 facial ointment of the present invention
1, raw material and consumption:
Chinese medicine extract 10 ~ 30g
Emulsifing wax 300g
White beeswax 500g
Liquid paraffin 200g
Phenoxyethanol 10g
2, preparation method:
(1) each raw material is taken by above-mentioned component and consumption;
(2), after emulsifing wax, white beeswax, liquid paraffin and phenoxyethanol being mixed in proportion, being heated to 80 DEG C and making material dissolution complete, stir homogenizing 10 minutes, then start cooling;
(3) add Chinese medicine extraction raw material when temperature drops to 60 DEG C, constant temperature stirs and makes to be uniformly dispersed, and is then cooled to 20 DEG C of dischargings slowly.
Embodiment 12 different solvents extract is on the impact of tyrosinase activity in melanoma b16 cell
Melanin is a kind of biochrome synthesized in melanocyte, and its biosynthesis is the enzymatic reaction of a multi-step, and the enzyme of participation mainly comprises tryrosinase, tyrosinase related protein-1 and TRP-2.In melanic forming process, tryrosinase plays the effect of key enzyme (rate-limiting enzyme).Therefore, skin-whitening agents is exactly generation by acting on dermal melanin and metabolic process, the generation of check melanin and deposition, and the material meeting safety standard.In this test system, according to the description of embodiment (1) (mensuration/96 well plate method of tyrosinase activity), measure tryrosinase in cell and, to the catalytic reaction of its substrate TYR, evaluate Chinese medicine extract (embodiment 1 ~ 5 prepares) inhibitory action to melanoma b16 intracellular tyrosine enzymatic activity.
Different extract is to 10% suppression ratio (EC of tyrosinase activity in B16 cell
10) and 20% suppression ratio (EC
20) comparative analysis the results are shown in Table 1.Result shows, the whitening effect of Extraction solvent on extract has larger impact.With the increase of concentration of alcohol in Extraction solvent, the trend that the inhibition of extract to tyrosinase activity was once fallen after rising.Wherein with 80% ethanol extraction to the inhibition of tyrosinase activity for the best.Therefore, we choose embodiment 4(80% ethanol extraction) extract for preparing carries out further separation and purification, evaluates its white-skinned face function.
Table 1 different solvents extract is on the comparison of tyrosinase activity impact in B16 melanoma cells
| Extract | Extraction solvent | EC 10（μg/mL） | EC 20（μg/mL） |
| Embodiment 1 | Water | 323 | 380 |
| Embodiment 2 | 20% ethanol | 300 | — |
| Embodiment 3 | 50% ethanol | 48 | 100 |
| Embodiment 4 | 80% ethanol | 18 | 83 |
| Embodiment 5 | 95% ethanol | 37 | 100 |
| Arbutin | — | 72.7 | 129.6 |
The different eluting position of embodiment 13 is on the impact of tyrosinase activity in melanoma b16 cell
In this test system, according to the description of embodiment (1) (mensuration/96 well plate method of tyrosinase activity), in mensuration cell, tryrosinase is to the catalytic reaction of its substrate TYR, the Chinese medicine extract obtained embodiment 4 is further after macroporous resin adsorption, and different solvents eluate (embodiment 6 ~ 9 prepares) inhibitory action to melanoma b16 intracellular tyrosine enzymatic activity compares.
Different solvents eluate is to 20% suppression ratio (EC of tyrosinase activity in B16 cell
20) and 40% suppression ratio (EC
40) comparative analysis the results are shown in Table 2.Result shows, after macroporous resin adsorption purification, different solvents eluate is all better than overall thing to the inhibition of tyrosinase activity.Meanwhile, the ethanol of more than 50% all can eluting be by the active site adsorbed effectively, and difference is little.Based on the principle of economy, we choose the method for the ethanol elution of 50% to carry out sample preparation.
The different eluting position of table 2 Chinese medicine composition extract is on the comparison of tyrosinase activity impact in B16 melanoma cells
| Extract | Eluting solvent | EC 20（μg/mL） | EC 40（μg/mL） |
| Embodiment 4 | — | 83 | — |
| Embodiment 6 | 20% ethanol | 51 | 78 |
| Embodiment 7 | 50% ethanol | 33 | 46 |
| Embodiment 8 | 80% ethanol | 33 | 44 |
| Embodiment 9 | 95% ethanol | 39 | 46 |
Embodiment 14 extracting solution is on the impact of tyrosinase activity inhibition and melanin content in B16 melanoma cells
(1) solution being mixed with 1mg/mL in the cell culture fluid (DMEM) that Chinese medicine composition extract (embodiment 7 prepares) is dissolved in containing the α-MSH of 10nM is accurately taken, through 0.2 μm of filtering with microporous membrane, 20 μ g/mL are diluted to the culture fluid of the α-MSH containing 10nM, 30 μ g/mL, 40 μ g/mL, the solution of 50 μ g/mL and 60 μ g/mL.Prepare the H-89 solution of 5 μMs with the cell culture fluid (DMEM) of the α-MSH containing 10nM simultaneously.
(2) according to described in above-mentioned embodiment (2) and embodiment (3), measure embodiment 7 and prepare extract impact on tyrosinase activity inhibition and melanin content in B16 melanoma cells under variable concentrations.
The extracting solution of variable concentrations the results are shown in Figure 1 to the Inhibition test of tyrosinase activity in B16 melanoma cells, and it the results are shown in Figure 2 to the experiment of B16 cell amount.Result shows, along with the increase of extract concentration, in cell, the activity of tryrosinase reduces gradually, and melanic synthesis reduces gradually, proves that this extract has obvious white-skinned face function.
Embodiment 15 extracting solution is to the inhibition of tyrosinase activity in human melanocytes HEMn
(1) accurately take Chinese medicine composition extract (embodiment 7 prepares) and be dissolved in the solution being mixed with 1mg/mL in cell culture fluid (M254), through 0.2 μm of filtering with microporous membrane, 5 μ g/mL are diluted to, the solution of 10 μ g/mL and 20 μ g/mL with culture fluid.Simultaneously not add the culture fluid of extract as blank, with Arbutin (54.5 μ g/mL and 109 μ g/mL) for positive control;
(2) according to described in above-mentioned embodiment (4), the extracting solution (embodiment 7 prepares) of variable concentrations is measured to tyrosinase activity inhibition in HEMn human melanocytes.
The extracting solution of variable concentrations the results are shown in Figure 3 to the Inhibition test of tyrosinase activity in HEMn human melanocytes.Result shows, along with the increase of extract concentration, in cell, the activity of tryrosinase reduces gradually, proves that this extract has obvious white-skinned face function.
The activity rating of the removing DPPH free radical of embodiment 16 extracting solution
(1) accurately take Chinese medicine composition extract (embodiment 7 prepares) and be dissolved in the storing solution being mixed with 10mg/mL in the methanol of 50%, then become the solution of 2 μ g/mL ~ 80 μ g/mL with the methanol dilution of 50%;
(2) accurately take rutin (positive control) and be dissolved in the storing solution being mixed with 10mg/mL in the methanol of 50%, then become the solution of 2 μ g/mL ~ 120 μ g/mL with the methanol dilution of 50%;
(3) accurately draw 50 μ L mensuration liquid or 50% methanol (blank) in 96 orifice plates, add the DPPH methanol solution of 150 μ L0.15M, hatch 30min in 25 DEG C of lucifuges;
(4) under 517nm wavelength, absorbance is measured, by following formulae discovery Chinese medicine composition extract and rutin to the Scavenging activity of DPPH free radical:
DPPH free radical scavenging activity (%)=(blank group-medicine group)/blank group × 100%
Oxidation Resistance Test the results are shown in Figure 4, along with the increase of concentration, the ability of Chinese medicine composition extract to DPPH free radical scavenging improves (IC gradually
50value is 7.02 ± 0.15 μ g/mL, Fig. 4 A).With positive control drug rutin (IC
50value is 10.64 ± 1.50 μ g/mL, Fig. 4 B) to compare, this extract has obvious DPPH radical scavenging activity.
The removing ABTS of embodiment 17 extracting solution
●+the activity rating of free radical
(1) accurately take Chinese medicine composition extract (embodiment 7 prepares) and be dissolved in the storing solution being mixed with 10mg/mL in the methanol of 50%, then become the solution of 5 μ g/mL ~ 200 μ g/mL with the methanol dilution of 50%;
(2) accurately take rutin (positive control) and be dissolved in the storing solution being mixed with 10mg/mL in the methanol of 50%, then become the solution of 5 μ g/mL ~ 500 μ g/mL with the methanol dilution of 50%;
(3) ABST accurately taking 8mg is dissolved in the water of 1mL, obtains solution A; The potassium persulfate accurately taking 13.2g is dissolved in the water of 10mL, obtains B solution; After the mixing of the B solution of the solution A of 0.5mL and 0.5mL, shading reaction 12 ~ 16h under room temperature.Reacted solution with water is diluted to debita spissitudo (absorbance under 734nm wavelength is 0.7 ± 0.02), obtains ABTS
●+solution;
(4) accurately draw 20 μ L mensuration liquid or 50% methanol (blank) in 96 orifice plates, add 180 μ LABTS
●+solution, hatches 5min in 25 DEG C of lucifuges;
(5) under 734nm wavelength, absorbance is measured, by following formulae discovery Chinese medicine composition extract and rutin to ABTS
●+the Scavenging activity of free radical:
ABTS
●+free radical scavenging activity (%)=(blank group-medicine group)/blank group × 100%
Oxidation Resistance Test the results are shown in Figure 5, along with the increase of concentration, Chinese medicine composition extract is to ABTS
●+the ability of free radical scavenging improves (IC gradually
50value is 6.13 ± 0.39 μ g/mL, Fig. 5 A).With positive control drug rutin (IC
50value is 9.81 ± 1.67 μ g/mL, Fig. 5 B) to compare, this extract has obvious ABTS
●+radical scavenging activity.
Embodiment 18 extracting solution absorbs ultraviolet effect assessment
Ultraviolet in sunlight is the electromagnetic wave of 200 ~ 400nm, wherein the ultraviolet in the UVB district of 280 ~ 320nm and the UVA district of 320 ~ 400nm, and skin can be made to shine red, tanned, sunburn.Add a certain amount of sunscreen in cosmetics, can prevent to reduce ultraviolet to the damage of human body.
According to the scope of ultraviolet place wave band, measure testing sample in the ultraviolet absorptivity of 280 ~ 400nm wave band, evaluate the effect (QBT2334-1997 ultraviolet spectrophotometer method qualitative determination cosmetics middle-ultraviolet lamp absorbent, People's Republic of China's light industry standard) of the anti-ultraviolet of Chinese medicine composition extract (embodiment 7 prepares).
Experimental procedure:
(1) take the Chinese medicine composition extract (embodiment 7 prepares) of 1mg, add 95% ethanol 1mL and dissolve, sample thief 25 μ L, 10 μ L 95% ethanol dilution is settled to 1mL as sample to be tested (25 μ g/mL, 10 μ g/mL) respectively;
(2) with 95% ethanol for blank, measure the absorption at 200 ~ 400nm wavelength of Chinese medicine extract solution in ultraviolet spectrophotometer;
(3) if instrument has absworption peak in this wave-length coverage, this sample containing ultraviolet absorber, without absworption peak then this sample do not contain ultraviolet absorber.
As shown in Figure 6, Chinese medicine composition extract (10 μ g/mL) has good absorption to the ultraviolet of 200 ~ 400nm wave band to experimental result, illustrates that Chinese medicine composition extract still has good sun-proof result under lower concentration.
The skin irritation evaluation of embodiment 19 extracting solution
According to chemicals toxicological evaluation program and experimental technique the 6th part: acute skin irritation/corrosion test (GBZ/T240.6-2011, People's Republic of China's Occupational sanitary standard) method, evaluate Chinese medicine composition extract (embodiment 7 prepares) as safety during preparation for external application to skin raw material.
Experimental procedure:
(1) take Chinese medicine composition extract (embodiment 7 prepares), obtain the solution of 3% with PEG400 dissolving or be prepared into by the method for embodiment 10 emulsifiable paste that content is 3%;
(2) choose the albino guinea-pig (8, male and female half and half) that body weight is about 300g, by standard conditions, single cage is raised.Before experiment, reject the hair of Cavia porcellus spinal column both sides, after recovering 24h, test;
(3) guinea pig back skin is divided into 4 regions, each region about 3 × 3cm, as shown in Figure 7 A, respectively by PEG400 solvent, containing the PEG400 solution of 3% Chinese medicine composition extract; Blank emulsifiable paste, the emulsifiable paste containing 3% Chinese medicine composition extract is applied to guinea pig back, fixes with gauze and oilpaper.Employing closure is tested, and the application time is 4h.After experiment terminates, remove residual given the test agent with warm water;
(4), after clean skin, in the dermoreaction that 1,24,48,72h observes recipient site, carry out dermoreaction scoring by table 3, carry out overall merit with the meansigma methods of animal subject integration, judge skin irritation intensity by table 4.
Table 3 skin wound repair is marked
Table 4 skin irritation strength grading standard
Experimental result as shown in Figure 7 B, is not observed Chinese medicine composition extract and is caused any skin wound repair in whole experimentation, illustrates that Chinese medicine composition extract uses as preparation for external application to skin raw material, has good safety.
Claims (14)
1. a Skin-care Chinese medicine compositions, is characterized in that, this Chinese medicine composition is made up of Pseudobulbus Bletillae (Rhizoma Bletillae), Pericarpium Granati, Semen Benincasae and Rhizoma Typhonii.
2. the Chinese medicine composition according to claim 1-, is characterized in that, the weight proportion of described Pseudobulbus Bletillae (Rhizoma Bletillae), Pericarpium Granati, Semen Benincasae and Rhizoma Typhonii is 1:1:1:1.
3. a skin care product additive for skin nursing, it comprises the Chinese medicine composition according to any one of claim 1 – 2, and acceptable adjuvant in skin care field.
4. prepare a method for the extract of Chinese medicine composition according to claim 1 and 2, specifically comprise the steps:
(1) described Pseudobulbus Bletillae (Rhizoma Bletillae), Pericarpium Granati, Semen Benincasae and Rhizoma Typhonii is taken according to 1:1:1:1 weight proportion;
(2) by after the water of medicinal at least 5 times of bulking values or alcohol solution dipping, heating and refluxing extraction;
(3) medicinal residues use the water of at least 5 times of bulking values or alcoholic solution heating and refluxing extraction 1 time again, merge extracted twice liquid;
(4) after being cooled to room temperature, with filter paper or frit, get filtrate and be concentrated into suitable volume;
(5) concentrated solution is added to after static adsorption reaches balance in macroporous resin column, is eluted to deionized water colourless;
(6) with ethanol elution, the concentration of ethanol is 20% ~ 95%;
(7) collect ethanol elution, be evaporated to after removing ethanol completely, with appropriate water dissolution extract, obtain Chinese medicine extract powder through lyophilization.
5. method according to claim 4, is characterized in that, the Chinese medicine extraction concentrated solution that described step (4) obtains, need through macroporous resin adsorption purification.
6. method according to claim 5, is characterized in that, resin absorption thing in described step (5), need use ethanol elution, the concentration of ethanol is 20% ~ 95%, obtains extract powder finally by lyophilization.
7. the extract of Chinese medicine composition prepared of method according to claim 4.
8. the extract of claim 7 is preparing the purposes in external-use skin care preparation.
9. purposes according to claim 8, is characterized in that, described external-use skin care preparation is emulsifiable paste, ointment, facial cream, essence or cleansing milk.
10. the purposes of Chinese medicine extract in the external-use skin care preparation preparing white-skinned face function that according to any one of claim 4 – 6, method prepares.
The purposes of the Chinese medicine extract that according to any one of 11. claim 4 – 6, method prepares in the external-use skin care preparation preparing antioxidation, senile-resistant efficacy.
12. the purposes of the Chinese medicine extract that method according to any one of claim 4-6 prepares in the anti-ultraviolet external-use skin care preparation of preparation.
13. purposes according to any one of claim 10-12, it is characterized in that, described external-use skin care preparation is emulsifiable paste, ointment, facial cream, essence or cleansing milk.
14. 1 kinds of skin care product additive with skin nursing effect, it comprises Chinese medicine extract and the acceptable adjuvant of skin care field that in claim 4 – 6, described in any one, method prepares.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410417672.2A CN105362676A (en) | 2014-08-22 | 2014-08-22 | Preparation method and application of traditional Chinese medicine composition having multiple effects for skin care |
| TW104127120A TW201613555A (en) | 2014-08-22 | 2015-08-20 | Preparation method of herbal composition with multi-functional skin protection and application thereof |
| HK16106014.1A HK1217919A1 (en) | 2014-08-22 | 2016-05-26 | A herb composition with multiple skin care effect and methods for preparation thereof and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410417672.2A CN105362676A (en) | 2014-08-22 | 2014-08-22 | Preparation method and application of traditional Chinese medicine composition having multiple effects for skin care |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105362676A true CN105362676A (en) | 2016-03-02 |
Family
ID=55365625
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410417672.2A Withdrawn CN105362676A (en) | 2014-08-22 | 2014-08-22 | Preparation method and application of traditional Chinese medicine composition having multiple effects for skin care |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN105362676A (en) |
| HK (1) | HK1217919A1 (en) |
| TW (1) | TW201613555A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520671A (en) * | 2016-11-24 | 2017-03-22 | 扬州大学 | In vitro isolated culture method for rabbit melanophore |
| CN107412103A (en) * | 2017-08-16 | 2017-12-01 | 佛山实瑞先导材料研究院（普通合伙） | A kind of antioxidant and its application |
-
2014
- 2014-08-22 CN CN201410417672.2A patent/CN105362676A/en not_active Withdrawn
-
2015
- 2015-08-20 TW TW104127120A patent/TW201613555A/en unknown
-
2016
- 2016-05-26 HK HK16106014.1A patent/HK1217919A1/en unknown
Non-Patent Citations (3)
| Title |
|---|
| 中华人民共和国卫生部: "化妆品卫生要求", 《化妆品卫生规范》 * |
| 无: "钱王红白散", 《医学文选》 * |
| 颜廷梅: "中西医结合治疗黄褐斑128例", 《徐州医学院学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520671A (en) * | 2016-11-24 | 2017-03-22 | 扬州大学 | In vitro isolated culture method for rabbit melanophore |
| CN107412103A (en) * | 2017-08-16 | 2017-12-01 | 佛山实瑞先导材料研究院（普通合伙） | A kind of antioxidant and its application |
Also Published As
| Publication number | Publication date |
|---|---|
| HK1217919A1 (en) | 2017-01-27 |
| TW201613555A (en) | 2016-04-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104013566B (en) | A kind of Chinese medicine composition with white-skinned face function, its preparation method and its cosmetic applications | |
| CN107714537B (en) | Composition and facial mask preparation containing same | |
| CN112494406A (en) | Whitening and freckle-removing cosmetic and preparation method thereof | |
| CN110090191B (en) | After-sun repair composition and cosmetic thereof | |
| CN106456526A (en) | Cosmetic composition for skin whitening and wrinkle alleviation, containing, as active ingredients, white rose extract and gartanin derivative compound isolated therefrom | |
| KR101073678B1 (en) | Shampoo composition using medical herbs and manufacturing method thereof | |
| KR100762965B1 (en) | Preparation methods of Panax ginseng extract increased contents of physiologically active substances Rb1, Rb2, Rc and Rd | |
| CN108635308A (en) | A kind of moisturizing radiation-resisting whitening skin care item and preparation method thereof | |
| JP2017507187A (en) | Cosmetic composition for skin whitening | |
| CN104027280B (en) | A kind of delay aging drug compositions and the application in cosmetics thereof | |
| CN103751552B (en) | A kind of Chinese medicine composition and preparation method and application with senile-resistant efficacy | |
| KR101474340B1 (en) | Anti-aging cosmetic composition comprising herb ferment extract | |
| KR20130031988A (en) | Skin external composition containing floral ginsenoside | |
| CN105362676A (en) | Preparation method and application of traditional Chinese medicine composition having multiple effects for skin care | |
| KR101639578B1 (en) | Cosmetic composition containing Ocimum basilicum seed extract | |
| KR101227701B1 (en) | Cosmetic composition for improving skin comprising HC-6 complex extracts | |
| KR101427573B1 (en) | Cosmetic composition for improving skin acne containing ginseng fruit extracts | |
| CN108403532B (en) | A kind of skin conditioner comprising ginseng extract | |
| CN106580833A (en) | Whitening moisturizing cream | |
| EP3552616B1 (en) | Hydrolysate of water extract of syzygium samarangense, and preparation process and use thereof | |
| CN109350578A (en) | Beautifying liquid and preparation method thereof and tyrosinase inhibitor | |
| CN113262191A (en) | Mother chrysanthemum skin-moistening cream and preparation method thereof | |
| KR101574555B1 (en) | Composition for enhancing hair growth containing ginsenoside Rg3, Rg5 and Rk1 as active ingredients | |
| CN104666147B (en) | A kind of Chinese herb compound for ultraviolet injury repair and its applied in cosmetics | |
| KR101427574B1 (en) | Cosmetic composition for antiinflammation containing ginseng fruit extracts |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1217919 Country of ref document: HK |
|
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20160302 |
|
| WW01 | Invention patent application withdrawn after publication | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: WD Ref document number: 1217919 Country of ref document: HK |