A kind of Chinese medicine composition and preparation method and application with senile-resistant efficacy
Technical field
The present invention relates to a kind of Chinese medicine composition, specifically a kind of there is senile-resistant efficacy Chinese medicine composition and preparation method and the application of its extract in cosmetics.
Background technology
Skin is the same with other organs, along with the age constantly increases, tends to old and feeble all gradually.The aging of skin is a kind of normal phenomenon, and from we are born, the phenomenon of skin aging just exists always, and the result of skin aging, except making the physiological function of skin and going down, the most important thing is that the appearance making people there occurs change.People, often through some external features of skin and hair, judge the age of a people, identity and status.Aging makes beauty lose lustre, and also can produce negative effect to the psychology of people, and therefore everybody wishes to keep appearance youth, perpetual youth.
Cause the reason of skin aging a lot, but all relevant with the free radical in human body after all.The aging course of organism is the result of the free radical accumulation that the histiocyte of body constantly produces, and free radical can cause DNA damage thus cause sudden change, induced tumor and human senescence.Free radical is the intermediate product of homergy, and its respond is very strong, and the many kinds of substance in cell can be made to be oxidized, infringement biomembrane.After free radical is formed, they can attack and injured cutaneous tissue cellularity, and cause following change: the cumulative bad oxidation variation 1. in long-chain molecule (as collagen protein, elastic fiber and chromosomal material), skin is followed the string and tension force gradually, and wrinkle constantly increases; 2. mucopolysaccharide (as hyaluronic acid) decomposes and intercellular substance (as ceramide) loss, makes xerosis cutis keratinization; 3. the accumulation of inert substance and the accumulation of aged pigment (as lipofuscin); 4. lipid peroxidation causes the change of cell membrane and plasma membrane; 5. the fibrosis of tremulous pulse and blood capillary; 6. enzyme activity reduces and immunity reduction, promotes old and feeble.
Prevention and delay skin aging are the important topics of cosmetic dermatology.Development effectiveness is obvious, safe, stable, the activity of fighting against senium thing of good absorbing, become the focus of current beauty industry, and the old and feeble active matter of plant origin, natural Traditional Chinese Medicine Anti is its main direction of studying.
Summary of the invention
Object of the present invention is just to provide the preparation method of a kind of natural safe potent delay aging drug compositions and this Chinese medicine composition extract.
To achieve the object of the present invention, present invention employs following technical scheme:
There is a Chinese medicine composition for defying age anti-senescence function, it is characterized in that the component comprising following percentage by weight: Pseudobulbus Bletillae (Rhizoma Bletillae) 10-50 part, Semen Vitis viniferae 10-50 part, Radix Et Rhizoma Fagopyri Tatarici 1-20 part, Radix Angelicae Sinensis 5-30 part, Cortex Mori 1-25 part.
The preparation method of above-mentioned delay aging drug compositions extract, it is characterized in that: take raw material of Chinese medicine Pseudobulbus Bletillae (Rhizoma Bletillae), Semen Vitis viniferae, Radix Et Rhizoma Fagopyri Tatarici, Radix Angelicae Sinensis, Cortex Mori by proportioning, suitable pulverizing, mixing, add Extraction solvent, 50-80 DEG C of heating extraction 0.5-3h, extracts 1-3 time, filters, merging filtrate, be evaporated to without alcohol taste, upper macroporous resin column purification, collect 30%-80% ethanol elution fraction, being evaporated to extracting solution with medical material ratio is 1: 0.5 ~ 1: 10, add 10%-60% butanediol to dissolve, filter, obtained Chinese medicine composition extract.The Extraction solvent used in the present invention can adopt water, ethanol, ethanol water.
The second object of the present invention is used in anti-aging cosmetics by the extract of above-mentioned delay aging drug compositions, makes stable in properties, respond well anti-aging cosmetics.
For realizing the second object of the present invention, present invention employs following technical scheme: the extract of above-mentioned Chinese medicine composition is used for preparing cosmetics, it is characterized in that its consumption is 0.01-25%, and preferable amount is 1-10% containing above-mentioned Chinese medicine composition extract in cosmetics.
The invention has the beneficial effects as follows: Pseudobulbus Bletillae (Rhizoma Bletillae) extract has very potent anti-free radical function and good astringing to arrest bleeding, detumescence and promoting granulation effect; Semen Vitis viniferae extract contains abundant anthocyanidin, vitamin E and aminoacid, is very potent free radical scavenger, but also has extraordinary uvioresistant, radioprotective and anti-allergic effects; Radix Et Rhizoma Fagopyri Tatarici is rich in the bioflavonoids such as Quercetin, has very strong free radical resisting effect and the effect of restraint of tyrosinase activity, can prevent freckle and senile plaque; Radix Angelicae Sinensis contains abundant ferulic acid, is very potent free radical scavenger, is also very potent tyrosinase inhibitor and sunscreen simultaneously, has potent defying age, whitening and sun-screening function; Cortex Mori contains the flavones ingredients such as Sang Su, has potent elimination free radical and restraint of tyrosinase active function, has effect of extraordinary reparation damaged tissues cell, desalination cicatrix, inducing diuresis to remove edema, whitening.All medicines share above, can Synergistic, play qi-supplementing, blood-engendering altogether, flourish merit of supporting skin, strong body constitution this.The potent scavenging free radicals of Chinese medicine composition extract energy of the present invention, restraint of tyrosinase are active, reduce melanin and generate, and accelerate skin surface cell renewal, delaying skin aging, make an addition in cosmetics, senile-resistant efficacy is remarkable, natural non-stimulated, the healthy good colour of skin.
Detailed description of the invention
Below by way of specific embodiment, the present invention is further illustrated, but these embodiments are just in order to illustrate, are not limitations of the present invention.
the preparation of embodiment 1 Chinese medicine composition extract (I)
Get Pseudobulbus Bletillae (Rhizoma Bletillae) 25g, Semen Vitis viniferae 30g, Radix Et Rhizoma Fagopyri Tatarici 8g, Radix Angelicae Sinensis 25g, Cortex Mori 12g, suitably pulverize, mixing, adds 50% ethanol 1.5L, 70 DEG C of heating extraction twice, each 2h, and filter, merging filtrate, is evaporated to without alcohol taste.Above extracting solution goes up AB-8 macroporous resin column purification again, and collect 70% ethanol elution fraction, under 60 DEG C of conditions, concentrating under reduced pressure obtains 80mL concentrated solution, then adds 20mL butanediol, filters, obtained 100mL traditional Chinese medicine composition extract liquid.
the preparation of embodiment 2 Chinese medicine composition extract (II)
Get Pseudobulbus Bletillae (Rhizoma Bletillae) 25g, Semen Vitis viniferae 25g, Radix Et Rhizoma Fagopyri Tatarici 5g, Radix Angelicae Sinensis 25g, Cortex Mori 20g, suitably pulverize, mixing, adds 50% ethanol 1.5L, 70 DEG C of heating extraction twice, each 2h, and filter, merging filtrate, is evaporated to without alcohol taste.Above extracting solution goes up AB-8 macroporous resin column purification again, and collect 70% ethanol elution fraction, under 60 DEG C of conditions, concentrating under reduced pressure obtains 80mL concentrated solution, then adds 20mL butanediol, filters, obtained 100mL traditional Chinese medicine composition extract liquid.
embodiment 3 anti-aging and nutritive frost
Table 1 provides the anti-aging and nutritive frost formula containing Chinese medicine composition extract of the present invention.
Table 1
Preparation method: A phase 80 DEG C dissolves mix homogeneously, B phase 80 DEG C dissolves mix homogeneously, adds B phase before Cyclomethicone emulsifying, and during emulsifying, B is added to A phase also homogenizing 5min, adds C phase also homogenizing 2 minutes, pack after ageing 24h when being cooled to 50 DEG C.
the common cream frost of comparative example 1
Table 2 provides the common cream frost formula not containing Chinese medicine composition extract of the present invention.
Table 2
Preparation method: A phase 80 DEG C dissolves mix homogeneously, B phase 80 DEG C dissolves mix homogeneously, adds B phase before Cyclomethicone emulsifying, and during emulsifying, B is added to A phase also homogenizing 5min, adds C phase also homogenizing 2 minutes, pack after ageing 24h when being cooled to 50 DEG C.
embodiment 4 experimental technique and anti-senescence function evaluation
1, the preparation of sample
Chinese medicine extract is made into the sample solution that concentration is 1%, tests below carrying out.
2, tyrosinase activity rejection ability is evaluated
According to table 3 application of sample, do not add tryrosinase, 1% arbutin is contrasted as one group of sample.Solution is placed in 37 DEG C of water-bath constant temperature 10min; Add tryrosinase, after solution being placed in 37 DEG C of water-bath constant temperature 10min, under 475nm, measuring its absorbance, and compare with 1% arbutin.
Table 3
|
Blank (ml) |
Blank (ml) |
Sample controls (ml) |
Sample (ml) |
Sample |
- |
- |
1.0 |
1.0 |
PBS(pH=6.8) |
2.5 |
2.0 |
1.5 |
1.0 |
TYR |
1.0 |
1.0 |
1.0 |
1.0 |
Tryrosinase |
- |
0.5 |
- |
0.5 |
Total amount |
3.5 |
3.5 |
3.5 |
3.5 |
By formula (1) calculation sample to the relative inhibition of tryrosinase (
i, be called for short suppression ratio, lower same).
(1)
In formula:
a 1,
a 2,
a 3,
a 4be respectively the absorbance of 1 group ~ 4 groups of solution.
3, ultra-oxygen anion free radical Scavenging activity is evaluated
Get the Tris-HCl buffer 4.5mL of 0.05mol/LpH=8.2, preheating 20min in 25 DEG C of water-baths.Add the pyrogallol solution of 1mL sample and 0.4mL25mmol/L again, after mixing, in 25 DEG C of water-baths, react 5min, add the HCl1.0mL cessation reaction of 8mol/L.Make reference with Tris-HCl buffer, survey absorbance at 299nm place.The solvent of blank 1mL sample carrys out alternative sample.Clearance rate (D) is calculated according to formula (2).
Ultra-oxygen anion free radical clearance rate (%)=[1-(A
2/ A
1)] × 100%(2)
In formula
a 1for the absorbance of blank;
a 2for the absorbance of medicine.
4, to the Scavenging activity evaluation of hydroxy radical
2mmol/LFeSO is added successively in 25mL color comparison tube
43mL, 1mmol/LH
2o
23mL, shakes up, and then adds 6mmol/L salicylic acid 3mL, shakes up, take out after 37 DEG C of heating in water bath 15min, survey its absorbance; Add certain density liquid to be measured respectively, shake up, continue heating in water bath 15min, take out and survey its absorbance.Following formula is the clearance rate of liquid to be measured to hydroxy radical (OH):
Scavenging action to hydroxyl free radical (%)=[A
1-A
2-(A
1-A
3)] ∕ A
1× 100%(3)
A in formula
1for not adding the absorbance of reaction system before medicine; A
2for the absorbance of system after medicine removing OH; A
3for the absorbance of system after blank removing OH.
5, experimental result
Anti-senescence function evaluation experimental is as shown in table 4.
Table 4
Experimental project |
Embodiment 1 |
Embodiment 2 |
To inhibitory activity against tyrosinase |
78.9 |
84.2 |
To superoxide anion clearance rate |
91.1 |
89.6 |
To Scavenging action to hydroxyl free radical |
92.4 |
90.5 |
Experiment shows, the potent scavenging free radicals of Chinese medicine composition energy of the present invention, and restraint of tyrosinase is active effectively, has good anti-senescence function.
embodiment 5 normal individual trial evaluation experimental
1, experimental technique
Cream frost prepared by embodiment 3 and comparative example 1 carries out normal individual trial evaluation respectively.Choose the volunteer 40 in 30-50 year, be women.Using method is use after clean of every day, twice daily (sooner or later respectively once), and left face uses the cream frost prepared by embodiment 3, the cream frost that right face use is prepared by comparative example 1.Experiment periods is one month, uses the colour of skin of observing left face and right face after month.
2, experimental result
Use after one month and find, have 34 user to employ the colour of skin of the colour of skin significantly better than the right face of the cream frost employed by comparative example 1 preparation of the left face of the cream frost prepared by embodiment 3, effective percentage reaches 85%.