KR101574555B1 - Composition for enhancing hair growth containing ginsenoside Rg3, Rg5 and Rk1 as active ingredients - Google Patents

Abstract

More particularly, the present invention relates to a cosmetic composition for promoting hair growth, hair growth, and hair loss, which contains ginsenosides Rg3, Rg5 and Rk1 as an active ingredient. The cosmetic composition of the present invention is excellent in hair growth, hair growth promoting effect and hair loss prevention effect, and is safe for long-term use, and is useful as a cosmetic for hair care.

Description

[0001] The present invention relates to a cosmetic composition for promoting hair growth, hair growth and hair loss comprising ginsenosides Rg3, Rg5 and Rk1 as an active ingredient,

More particularly, the present invention relates to a cosmetic composition for preventing hair growth, hair growth, and hair loss. More specifically, the present invention relates to a cosmetic composition containing ginsenosides Rg3, Rg5 and Rk1 as an active ingredient and having excellent hair growth, hair growth promoting effect and hair loss prevention effect, Hair growth promotion, and hair loss prevention.

Among various diseases related to the scalp, alopecia is a scalp pathology which is not easy to treat and is often encountered in the vicinity. In contemporary society, which emphasizes appearance, hair loss is the biggest obstacle to external expression and it is recognized as a field of disease and various researches and developments are being made. Due to increased stress and westernized diet and external causes, male hair loss is more than 30% of males in their 40s and 50s, and female hair loss prevalence is estimated to be more than 5%. In addition, the age at which hair loss is manifested in men and women after middle-aged or menopausal years is lowered to the 20s and 30s.

Various attempts have been made to treat alopecia such as surgical procedures such as wig, implant surgery, and drug therapy. The surgical procedure is excellent in terms of effectiveness, but it has a problem that it is a temporary treatment method and can not be a fundamental treatment method in addition to the disadvantage that the cost and the operation procedure are difficult and the economic and psychological burden of the patient is very great.

Minoxidil, which is widely used for pharmacotherapy, was originally used as a therapeutic agent for hypertension, but it was started to be used as a hair growth promoting agent due to the fact that hair growth was reported as a side effect. Minoxidil has been applied to male hormonal alopecia, which has been shown to be effective in treating both alopecia areata, but it is less effective for alopecia areata. It is also unsuitable for long-term use due to potential side effects such as hypotonia caused by antihypertensive action, hypotension, tachycardia, and cardiovascular complications. Propecia, which inhibits the production, binding and metabolism of the androgen, the male hormone, can lead to feminization of males and the problem of birth defects in fertile women. As such, drug therapy using a chemical causes imbalance of homeostasis in the body, and various unexpected side effects occur. Therefore, studies on a hair growth agent using natural materials without side effects have been actively pursued.

Recently, herbal shampoos and hair growth agents containing a composition for hair growth using a mixed extract of various herbal medicines including ginseng as a raw material have attracted much attention. These compositions, however, do not provide systematic verification of the active ingredients, as they provide nutrients to hair follicles and hair follicles and improve blood flow to prevent hair loss and promote hair growth.

It is known that ginseng has no harmful effect on the human body even when taken over a long period of time, and has various effects such as fatigue recovery, anti-aging, tonic and anticancer . Ginseng contains about 30 kinds of saponins that show unique bioactivity. In addition, there is a difference in the total saponin content depending on the kind of ginseng, processing method, and site, and the distribution of saponins is also large. Saponin is a kind of compound called gypsum, which is known to exist not only in ginseng but also in various plants such as ginseng, bellflower, licorice, soybean, aloe, and sea cucumbers and starfish.

Since ginsenosides isolated from red ginseng are very low in yield and expensive compared with other materials, many studies have recently been made on the mass production of certain components of saponin.

The pharmacological effects of various components of ginsenoside isolated from red ginseng and ginseng have been scientifically proven and many developments have been made on hair cosmetic compositions and cosmetics containing them, There are not many studies on the complex action of certain ginsenosides. In other words, hair cosmetics and cosmetics including the existing ginenoside have been developed with a comprehensive purpose for a very wide range of functions such as hair growth, hair growth, wool or skin useful functions, and most of the cosmetic compositions containing ginenoside The ginseng and red ginseng contained a complex extract of ginsenoside as a result, most of them were contained as a part of the active ingredient. Also, when using a single substance of a particular ginsenoside or using a mixture, a common mixture such as crude saponin was used at a specific ratio.

Korean Patent Laid-Open Publication No. 10-2013-0049072 "Composition for applying scalp or hair for promoting hair growth using rice fermented product and method for producing the same" includes' Red ginseng, Sechangpo, Aloe, Discloses a composition for applying scalp or hair for promoting hair growth, which comprises a mixture of a bamboo extract and a red ginseng extract as an active ingredient, and a composition for preventing hair loss or hair growth, which comprises Korean Patent No. 10-1233910, The mixture is prepared by mixing a mixture of a bamboo extract and a red ginseng extract in a weight ratio of 9: 1 (from a bamboo fruit extract: red ginseng extract) to 7: 3 (from a bamboo fruit extract: red ginseng extract) .

The present inventors selected ginsenosides Rg3, Rg5 and Rk1 through various hair growth promoting and hair loss prevention experiments using various ginsenosides, and tried to find the best mixing ratio by mixing them in various ratios. As a result, the present invention has been completed.

It is an object of the present invention to provide a cosmetic composition which is excellent in hair growth, hair growth promoting and hair loss prevention effect by effectively blending specific components of ginsenosides derived from red ginseng and ginseng.

According to the present invention, there is provided a cosmetic composition for promoting hair growth, hair growth, and hair loss comprising ginsenosides Rg3, Rg5 and Rk1 as an active ingredient. The ginsenosides Rg3, Rg5 and Rk1 are mixed at a weight ratio of 1 to 10: 10 to 1: 1, respectively. Preferably, the ginsenosides Rg3, Rg5 and Rk1 are 2 to 8: 1: 1 by weight.

The ginsenosides Rg3, Rg5 and Rk1 are contained in an amount of 0.005 to 50% by weight, preferably 0.01 to 20% by weight, based on the total weight of the cosmetic composition.

The cosmetic composition of the present invention containing ginsenosides Rg3, Rg5 and Rk1 has no adverse effects on the human body and is not toxic and has excellent 5α-reductase inhibitory activity and thus has excellent hair growth, hair growth promoting effect and hair loss prevention effect, And can be useful as a cosmetic for preventing hair loss.

1 is a graph showing the results of 5α-reductase inhibition activity of a mixture of ginsenosides according to the present invention.
2 and 3 are graphs showing hair density and hair thickness according to clinical tests of the cosmetic composition according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a technique using a mixture of ginsenosides Rg3, Rg5 and Rk1 as a cosmetic material to promote hair growth or hair growth and prevent hair loss.

Ginsenosides Rg3, Rg5 and Rk1 were selected from a variety of ginsenosides for promoting hair growth and preventing hair loss, and they were mixed at various ratios to find the best mixing ratio.

The ginsenoside contained in the cosmetic composition of the present invention as an active ingredient is saponin which is a kind of complex carbohydrate contained in ginseng. As ginseng saponin components have been actively studied so far, many physiological activity mechanisms of each ginsenoside have been known. Among the various components of ginseng, ginsenoside Rg3 is a specific component not found in white ginseng but present only in red ginseng. The main saponin ginsenoside Rg3, which is converted during the production of red ginseng, has been reported to have physiological activity effects such as anti-cancer, anti-anemia, anti-pain, anti-obesity, anti-stress and immunity enhancement.

Ginsenoside Rg5 is a component mainly contained in red ginseng, which is known to have immunity and anti-cancer effects.

Ginsenoside Rk1 is a saponin contained in ginseng and black ginseng and is known to have anticancer and cognitive function improvement.

In the cosmetic composition of the present invention, the ginsenosides Rg3, Rg5 and Rk1 are preferably mixed at a weight ratio of 1: 10: 10 to 1: 1, more preferably 2: 8: 4 to 1: 1, The hair growth promoting effect and the hair loss prevention effect were the most excellent.

The mixture of ginsenosides Rg3, Rg5 and Rk1 as an active ingredient is contained in an amount of 0.005 to 50% by weight, more preferably 0.01 to 20% by weight, based on the total weight of the cosmetic composition. When the content is less than 0.005% by weight, the activity is weak, and when it is more than 50% by weight, the composition is unstable.

The formulations of the cosmetic composition of the present invention are not particularly limited and may have formulations such as hair conditioner, hair tonic, hair cream, hair lotion, hair oil, hair spray, shampoo, rinse and the like.

In the composition of each formulation, an arbitrary component can be selected and mixed depending on the formulation of the cosmetic composition, the purpose of use, and the like. For example, colorants, flavors, suspending agents, emulsifiers, solubilizers, stabilizers, isotonic agents, pH adjusting agents, viscosity adjusting agents, solvents and the like. In addition, the cosmetic composition for promoting hair growth, hair growth promoting hair loss and hair loss according to the present invention may further include other components having hair growth promoting effect and hair loss preventing effect.

The cosmetic composition containing the mixture of ginsenosides Rg3, Rg5 and Rk1 of the present invention as an active ingredient was excellent in 5α-reductase inhibitory activity. As a result of the clinical tests, hair density and hair thickness were improved and hair growth, hair growth promoting and hair loss It has been confirmed that it has an effect of prevention.

[Example]

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, these embodiments are merely examples for explaining the content and scope of the technical idea of the present invention, and thus the technical scope of the present invention is not limited or changed. It will be apparent to those skilled in the art that various changes and modifications can be made within the scope of the technical idea of the present invention based on these examples.

Example  1 to 6: Gin Senocide  Preparation of mixtures

Ginsenosides Rg3, Rg5 and Rk1 were purchased from Faces Biochemicals and mixed in the ratios shown in Table 1 below to prepare ginsenoside mixtures.

Comparative Examples 1 to 4: Preparation of ginsenoside mixture

Ginsenosides Rg3, Rg5 and Rk1 were mixed and prepared in the ratios shown in Table 1 below.

ingredient Example Comparative Example One 2 3 4 5 6 One 2 3 4 Rg3 (占 퐂 / ml) 70 50 60 80 50 60 100 70 70 70 Rg5 (占 퐂 / ml) 20 40 30 10 30 20 0 0 30 0 Rk1 ([mu] g / ml) 10 10 10 10 20 20 0 0 0 30

Test Example 1: 5α-reductase inhibitory activity test

The ginsenosides of the above Examples and Comparative Examples were dissolved in 50% ethanol and used in the test. 5α reductase inhibitory activity was measured by the method of Hartmann et al. It is a method to quantify metabolites by reacting with testosterone as a substrate. 1 mM nicotinamide adenine dinucleotide phosphate (Sigma, Co, Mo, USA) was dissolved in 0.5 ml of testosterone (Sigma, Co, Mo, USA), 1 ml of 10 M Tris-HCl buffer mL was added to initiate the reaction, and the reaction conditions were incubated at 37 ° C for 30 minutes. The reaction was stopped by adding 5 mL of dichloromethane, followed by centrifugation at 3000 rpm for 10 minutes. After removing the supernatant, the dichloromethane layer was left and left in a hood for one day to remove the solvent. The residue was quantitated by high performance liquid chromatography (HPLC) with 3 mL of methanol, and the inhibition rate was compared with the control . As a control, 50% ethanol without ginsenoside was used. Shim-pack ODS (4.6 μm, 250 × 4.6 mm) was used as a column for the HPLC measurement, and a methanol: H ₂ O mixture (65:35) was used as the mobile phase. The flow rate was 1 mL / min. The detection wavelength was detected at UV 254 nm and the injection volume was 20 μl.

Inhibition rate = (1 - the amount of DHT in the example, the comparative example / the amount of DHT in the control example) × 100

The results are shown in Fig. As shown in FIG. 1, it can be seen that the mixture of ginsenosides Rg3, Rg5 and Rk1 of the above example exhibits an inhibitory activity of 5α-reductase superior to that of a single ginsenoside or a mixture of two components. Examples 1 to 4 exhibited higher activity than Comparative Example 1 in which 100% of ginsenosides was most effective in preventing hair growth, promoting hair growth and preventing hair loss. Particularly, it can be seen that the inhibition rate particularly increased when the mixture was treated with the mixture of 7: 2: 1 ratio of ginsenosides Rg3, Rg5 and Rk1. Examples 5 and 6 showed activity lower than that of Comparative Example 1 but showed close activity and showed higher activity than the same amounts of Comparative Examples 3 and 4 in which two ginsenosides were mixed and showed that when three ginsenosides were mixed 5-α-reductase.

Example  7: Rg3 , Rg5 , Rk1  Preparation of mixture-containing shampoo

A shampoo containing a mixture of ginsenoside Rg3, Rg5 and Rk1 was prepared according to the conventional method according to the composition shown in Table 2 below.

(weight%) Comparative Example 5 Example 7 Ginsenoside Rg3, Rg5, Rk1 mixture
(Example 1) - 10 Ammonium lauryl sulfate /
Ammonium laureth sulfate 16 16 Trihydroxystearin 0.3 0.3 Cetyl alcohol 0.6 0.6 Cocamide eiei 1.0 1.0 Guarhydroxytrimonium chloride 0.2 0.2 Spices 1.0 1.0 antiseptic 2.0 2.0 Citric acid 0.05 0.05 Purified water to 100 to 100

Test Example 2: Clinical trial

Clinical trials were carried out using the shampoo compositions of Example 7 and Comparative Example 5 to confirm clinical effects of hair growth, hair growth promoting and hair loss prevention effects of the composition containing the ginsenosides Rg3, Rg5 and Rk1 of the above Examples.

Hair density measurement

15 subjects were randomly divided into the no-added group (Comparative Example 5) and the sample added group (Example 7) for 30 subjects having the initial hair loss phenomenon. The subjects were each shaved every morning with no added shampoo and sample added shampoo, and the parietal area was measured using the ARAMO HMS scalp measurement system for four weeks at intervals of one week.

The results are shown in Fig. As a result, 4 weeks after the non-addition group is 124.2 pcs / in cm ^2, but almost no change in 124.1 pcs / cm ^2, the sample addition group in at 123.9 pcs / cm ² 127.03 atoms / cm ² in hair density, as shown in FIG. 2 .

Hair thickness measurement

The thickness change of the hair was measured by the above method, and the result is shown in FIG. As can be seen in FIG. 3, the thickness of the hair was almost unchanged from 0.0801 mm to 0.0802 mm in the no-added group, and 0.0818 mm in the sample-added group.

Thus, it can be understood that the cosmetic composition containing the ginsenosides Rg3, Rg5 and Rk1 of the present invention has hair growth, hair growth promoting and hair loss prevention effects.

Claims (5)

A cosmetic composition for promoting hair growth, hair growth, and hair loss comprising a ginsenoside mixture of ginsenosides Rg3, Rg5 and Rk1 mixed at a ratio of 7: 2: 1, respectively, as an active ingredient. delete delete The cosmetic composition according to claim 1, wherein the ginsenoside mixture is contained in an amount of 0.005 to 50% by weight based on the total weight of the cosmetic composition. The hair cosmetic composition according to claim 1, wherein the cosmetic composition for promoting hair growth, hair growth and hair loss has a formulation of hair conditioner, hair tonic, hair cream, hair lotion, hair oil, hair spray, shampoo or rinse. And a cosmetic composition for preventing hair loss.

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