KR102322628B1 - A method for preparing wild ginseng cultured root extract with increased content of specific ginsenosides and cosmetic composition for relieving hair loss symptoms and promoting hair growth using ginsenoside complexes - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating a hair loss symptom and promoting hair growth using a wild ginseng cultured root extract with the increased content of specific ginsenosides which is composed mainly of a ginsenoside complex obtained from the wild ginseng culture root extract with the increased content of specific ginsenosides as a main component to exhibit the effect of alleviating the hair loss symptom and promote the hair growth, adds a Sophora flavescens extract with an increased active ingredient as a Demodex folliculorum remover to improve the scalp condition, and contains a Scoria extract capable of increasing the storage stability of the active ingredient derived from the Sophora flavescens extract.

Description

Method for preparing wild ginseng cultured root extract with increased content of specific ginsenosides and cosmetic composition for alleviating hair loss symptoms and promoting hair growth using ginsenoside complex for relieving hair loss symptoms and promoting hair growth using ginsenoside complexes}

The present invention purifies and separates specific ginsenosides from cultured wild ginseng root extracts obtained through high-pressure enzymatic decomposition after secondary steaming, and specific ginsenoside components purified and separated from cultured wild ginseng root extracts with increased specific ginsenoside content can exhibit the effect of alleviating hair loss or promoting hair growth by using the complex and it as a main component, and can improve the scalp by adding the high ginseng extract with increased active ingredient as a demodex remover, and can increase the storage stability of the active ingredient derived from the high ginseng extract It relates to a cosmetic composition for alleviating hair loss symptoms and promoting hair growth using wild ginseng cultured root extract with an increased content of specific ginsenosides.

In general, the causes of baldness or hair loss include the action of male hormones in organs such as the hair root and sebaceous glands, a decrease in blood flow to the hair follicles, excess sebum secretion, superoxide production, scalp abnormalities due to bacterial growth, genetic factors, aging, etc. this is mentioned However, a clear cause of hair loss has not yet been identified, and more and more people are suffering from alopecia in recent years, and the age group is also decreasing.

Regarding the treatment of alopecia, there is still no clear effect, and based on previous experience, physical stimulation was applied to the head with a brush or the like, or a compound having an action of removing or alleviating the cause was used.

The human body has more than 1.3 million hairs and more than 100,000 hairs. These hairs go through three phases of growth, regression, and resting phase, and each hair operates in an independent cycle. Hair loss, which means a state in which there is less or no hair or hair than normal for some reason, can be divided into a general hair loss phenomenon that falls out in the resting state and a growth phase hair loss phenomenon that is an abnormal hair loss phenomenon. In general, alopecia refers to the latter.

Conventionally, there are preparations based on female hormones in relation to hormone theory in the treatment of such alopecia, but there are reports of effects as well as skin inflammation and side effects caused by hormone administration, so their use is decreasing.

In addition, as a recent hair growth agent, a formulation containing minoxidil of Upzon of the United States and tricosaccharide of Crinos of Italy, etc. as a main ingredient is being distributed, but side effects such as absence of a clear effect and human safety are emerging.

That is, the conventionally widely used hair growth agent uses a chemical substance, which temporarily expands capillaries through blood flow to stimulate the hair root, so it is not only temporary, but also has an effect on hair loss due to excessively secreted sebum. have.

Korean Patent Registration No. 10-1869850

The present invention is to solve the above problems, and after the second steaming, the wild ginseng cultured root extract obtained through high-pressure enzymatic decomposition process is separated and purified to obtain specific ginsenoside Rg3, Rg4 and Rg6, respectively, and then, it is obtained in a specific ratio. By preparing a cosmetic composition comprising the mixed ginsenoside complex, oximarine, a component derived from ginseng extract effective in removing Demodex, and scoria extract to improve the storage safety of oxymatrine, a ginsenoside complex can exhibit the effect of alleviating hair loss symptoms or promoting hair growth by An object of the present invention is to provide a cosmetic composition for hair that is excellent in alleviating hair loss symptoms and promoting hair growth using wild ginseng culture root extract with increased senoside content and is safe even for long-term use.

As a means for solving the above problems, the composition of the present invention is 0.001 to 15% by weight of the ginsenoside complex; 0.01 to 10% by weight of high ginseng extract; 20-40 wt% of surfactant; 30-70% by weight of purified water; 0.01-5% by weight of a moisturizer; 0.001 to 10% by weight of ceramide; 0.01 to 2% by weight of a preservative; and 0.5 to 3% by weight of fragrance.

The ginsenoside complex according to the configuration of the present invention is obtained by obtaining, respectively, ginsenoside Rg3, Rg4 and Rg6 from any one of wild ginseng cultured root, red ginseng, ginseng or black ginseng, which has been subjected to high-pressure enzymatic decomposition after steaming twice. ginsenosides Rg3, Rg4 and Rg6, respectively, 1-3: 1-3: characterized in that it is made by mixing in a weight ratio of 1-3.

The high ginseng extract according to the configuration of the present invention is characterized in that it contains oximarine in an amount of 0.25 to 0.41% by weight by steaming twice before extraction.

The preservative according to the configuration of the present invention uses scoria extract to increase the storage stability of oxymatrine derived from ginseng extract, and 0.5 to 5 parts by weight of scoria extract is added to the scoria extract for 12 hours at 45-60° C. It is characterized in that it is aged for a while, desalted to a salt concentration of 0.85 to 0.90%, and filtered.

The cosmetic composition according to the configuration of the present invention can remove Demodex and improve the stability of oximarine.

The present invention is a ginsenoside complex obtained from a wild ginseng cultured root extract with an increased content of specific ginsenosides, and by using it as a main component, it can exhibit the effect of alleviating hair loss symptoms or promoting hair growth, and adding a high ginseng extract with an increased active ingredient as a Demodex remover Thus, the scalp can be improved, and the storage stability of the active ingredient derived from the high ginseng extract can be increased by the Scoria extract.

Hereinafter, a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings in order to describe in detail enough that a person of ordinary skill in the art can easily carry out this invention. Other objects, including the object, action, effect, and operational advantages of this invention will become clearer by the description of the preferred embodiment.

For reference, the embodiments disclosed herein are only presented by selecting and presenting the most preferred embodiments to help those skilled in the art from among various possible implementations, and the technical spirit of the present invention is not necessarily limited or limited only by the presented embodiments. No, various changes, additions, and modifications including equivalents to substitutes are possible within the scope not departing from the technical spirit of the present invention.

In addition, the terms or expressions used in the specification and claims are defined on the basis of the principle that the inventor can appropriately define the concept of a term in order to best describe his invention, and It should not be construed as being limited to a dictionary meaning, but should be interpreted as meaning and concept consistent with the technical idea of this invention. As an example, the singular expression includes the plural expression unless the context clearly dictates otherwise.

According to the present invention, the method for producing a wild ginseng root extract having an increased content of specific ginsenosides and the cosmetic composition for alleviating hair loss symptoms and promoting hair growth using the ginsenoside complex contains ginsenosides Rg3, Rg4 and Rg6, respectively, 1-3: 1-3: Contains 0.001-15 wt%, preferably 0.003-10 wt%, based on the total weight of the cosmetic composition as an active ingredient of the complex formed by mixing in a weight ratio of 1-3.

Ginsenoside is a type of saponin, a type of glycoside. It is derived from the roots, stems, leaves, bark, seeds of plants and is also contained in the body of some echinoderms (starfish, sea cucumber). have. Saponin is known as a component that enhances the immune system of the human body, and ginsenoside, a type of saponin contained in ginseng, red ginseng, wild ginseng, old ginseng, and black ginseng, is particularly famous.

In particular, rare ginsenoside is a word that refers to a saponin contained only in ginseng and is used to distinguish it from saponins of other plants. Rare ginsenosides have a smaller material size than general ginsenosides and have structural characteristics that are relatively easy to absorb into the body. In addition, when regular ginsenosides are consumed, a small amount is converted to rare ginsenosides by the intestinal microflora and absorbed, but because there is a large individual difference, taking the rare ginsenoside form is more efficient in terms of absorption and bioavailability in the body. have.

The specific ginsenosides Rg3, Rg4, and Rg6 used in the present invention are specific ginsenosides extracted from cultured wild ginseng root, and the cultured wild ginseng root is cultured and manufactured in a clean room, so it is not exposed to pesticides and heavy metals at all. Have.

The main components of wild ginseng include saponin, a glycoside component, as well as nitrogen-containing compounds such as proteins, amino acids, nucleic acids, alkaloids, and fat-soluble components, such as fatty acids, essential oils, polyacetylene, phenolic compounds, phytosterol, and tere. As terpenoids and sugars, it contains a wide variety of components such as monosaccharides, oligosaccharides, polysaccharides, pectins, vitamins, minerals, and alkaloids.

In general, saponin components are widely distributed in the plant kingdom (about 750 plant species). Saponin is the name given to the substance that produces fine bubbles like soap in aqueous solution. In general, the saponin component lowers the surface tension of water, so it foams easily and shows hemolysis. However, it has been found that ginseng saponin is mild in weakness, non-toxic, and has almost no hemolytic action. In addition, the chemical structure of ginseng saponin is completely different from that of saponins found in other plants. So, to distinguish ginseng saponin from other plant-based saponins, it is called ginsenoside in the meaning of ginseng glycoside.

Ginsenosides include ginsenosides Rg1, Rg2, Rg3, Rg4. Various types such as Rg6, Rb1, Rb2, Rh1, Rh2, Rc, Rd, Re, and Ro exist, different types and amounts.

Ginsenoside Rg1 is known as a substance that strengthens immune function and has anti-fatigue action, Rg2 is known as a substance that inhibits platelet aggregation, and Rg3 has anti-anemia, anti-stress, immunity enhancement, cancer cell metastasis inhibition and hepatoprotective action. It is known as a substance that Rg4 is known to have anti-inflammatory and pulmonary inflammation-improving effects.

In addition, Rg5 and Rg6 are known to have very strong anti-inflammatory and anticancer effects, Rb2 is known to have anti-diabetic, anti-arteriosclerosis and immunomodulatory effects, and Ro is alcohol detoxification, anti-inflammatory and anti-hepatitis effects. It is known as a substance that Ginsenoside Rb1 is a type of protopanaxadiol belonging to the dammarane family among saponins. This compound has excellent central nervous system sedation and is known as an anti-inflammatory substance.

The cosmetic composition of the present invention is not particularly limited in its formulation, and includes hair tonic, hair essence (ampule), hair conditioner, hair cream, hair pack, hair oil, and hair spray. It may have formulations such as shampoo and conditioner, and may be formulated by selecting arbitrary ingredients according to the formulation or purpose of use of the cosmetic. For example, it may include a colorant, a flavoring agent, a suspending agent, an emulsifying agent, a solubilizing agent, a stabilizer, a pH adjusting agent, a viscosity adjusting agent, a solvent, and the like. In addition, other ingredients having a hair growth promoting effect or a hair loss symptom relief effect may be further included, and the mixing ratio of the main ingredients may be adjusted.

The ginsenoside complex contained in the cosmetic composition of the present invention may be extracted not only from cultured wild ginseng root, but also from red ginseng, ginseng, black ginseng, old ginseng, and the like.

(Production of wild ginseng cultured root extract - pretreatment process for high-pressure enzymatic digestion after secondary steaming)

First, 5 kg of cultured wild ginseng root was crushed and subjected to a primary steaming treatment for 8 hours at a temperature of 30° C. and a humidity of 40%. Next, the first steam-treated wild ginseng cultured root was placed in a freeze dryer and first dried at -40°C for 12 hours. Then, the primary dried wild ginseng cultured roots were subjected to a secondary steaming treatment for 8 hours at a temperature of 30° C. and a humidity of 40%. Next, the second steam-treated wild ginseng cultured root was placed in a freeze dryer and secondarily dried at -40°C for 12 hours.

A natural product powder was prepared by pulverizing a sample dried with a freeze dryer to a size of 60 to 120 mesh, respectively. In addition, the citric acid aqueous solution for use as an extraction solvent was prepared by diluting with distilled water until the pH of the 0.2M citric acid solution was 4.8. Then, 1 part by weight of natural product powder (cultivated wild ginseng powder) was added to 100 parts by weight of the prepared pH 4.8 citric acid aqueous solution to prepare an extraction mixture (sample 30 mg/citric acid solution 30 mL). Based on 30 mL of the above extraction mixture, 50 to 100 μL of Viscozyme (KTN02214) or Pectinex Ultra SP-L (KRN05658) from Novozymes (Bagsvaerd, Denmark), or a mixture of two enzymes (Viscozyme + Pectinex) is added to the extraction mixture.

The extract mixture prepared in this way was administered to a polypropylene bag (Nasco, WHIRL-PAK-4oz.), a plastic container for ultra-high pressure reactor, and sealed so that there are no air bubbles, to prepare a sample for high-pressure enzyme reaction treatment. Put the high-pressure reactor dedicated container containing the extract mixture containing the enzyme into the high-pressure reactor (Toyo Koatsu, TFS-2L), and set the pressure of the high-pressure reactor to 100 MPa, the temperature to 50 ° C, and the high-pressure treatment time to 8 hours. was operated. After 8 hours, the extraction mixture was recovered, and a sample was prepared in which 45 mL of ethanol was added based on 30 mL of the extraction mixture. The comparative group was used as a wild ginseng root extract solution extracted by immersion in a water-bath at 65° C. under atmospheric pressure (0.1 MPa) for 8 hours.

In order to detect the type and content of ginsenosides contained in the experimental group, which is a sample prepared by the above method and ultra-high pressure, and the comparative group, which is a sample subjected to atmospheric pressure, the indicator components of each sample were analyzed according to the following method. .

analysis procedure

1. Preparation of test solution

1) Accurately weigh about 1 g of the sample, put it in a 250 mL reflux flask, add 50 mL of 50% methanol solution, extract under reflux in a water bath at 70~80oC for 1 hour, cool, centrifuge, and take the supernatant (supernatant A) .

2) Add 50 mL of 50% methanol solution to the residue again, extract under reflux for 1 hour in a water bath at 70~80oC, cool and centrifuge to obtain a supernatant (supernatant B).

3) Combine supernatant solution A and supernatant solution B together, put it in a 250 mL concentration flask, and concentrate under reduced pressure at 60°C or less.

4) The concentrate is dissolved in 2 mL of a water-acetonitrile (80:20 V/V) mixed solvent, filtered through a 0.45 μm membrane filter, and used as a test solution for HPLC analysis.

2. HPLC analysis

20 μL of test solution and 20 μL of standard solution are injected into HPLC and analyzed according to the following conditions to obtain the peak area of each ginsenoside.

1) Column: ODS (4.6 mm × 250 mm) or equivalent or higher

2) Detector: UV (measurement wavelength: 203 nm)

3) Mobile phase: water-acetonitrile mixed solvent (flow: 1.6 mL/min)

3) Calculation of ginsenoside content

The amount of each ginsenoside contained in the sample is calculated according to the following formula.

[Equation 1]

Y=S(a*b) / A*0.001

Here, Y is the ginsenoside content, S is the concentration of individual ginsenosides in the test solution (μg/mL), a is the total amount of the test solution (mL), b is the dilution factor, and A is the sample amount (g). do.

The total content of ginsenosides of the sample treated with enzyme and ultra-high pressure, calculated in this way, was 27.61 mg/g, which increased by 22% compared to the ginsenoside content (22.63 mg/g) of the sample (comparative group) treated with atmospheric pressure. did.

In the same way as above, ginsenosides Rg3, Rg4 and Rg6 were obtained from the wild ginseng cultured root extract, respectively, and then ginsenosides Rg3, Rg4 and Rg6 were added to a stirrer at a ratio of 1-3: 1-3: 1-3, respectively. A ginsenoside complex was prepared by input and stirring at 500 to 1000 rpm for 5 to 8 hours.

The ginsenoside complex obtained by purifying and separating the cultured wild ginseng root prepared through the above pretreatment process is ginsenoside Rg3, Rg4, and Rg6, each of which is mixed in a weight ratio of 1-3: 1-3: 1-3. It contains 0.001 to 15% by weight of the senoside complex as an active ingredient based on the total weight of the cosmetic composition. More preferably, when the ginsenosides Rg3, Rg4 and Rg6 are mixed in a weight ratio of 2: 1: 1 or 3: 1: 1, respectively, 0.003 to 15% by weight of the total weight of the cosmetic composition is contained in the case of a ginsenoside complex The effect of promoting hair growth and alleviating hair loss symptoms was more excellent.

On the other hand, for the high ginseng extract according to the present invention, 5 kg of high ginseng root was crushed and subjected to a primary steaming treatment for 8 hours at a temperature of 15° C. and a humidity of 40%. Next, the first steamed ginseng root was put in a freeze dryer and first dried at -40°C for 12 hours. Then, the first dried ginseng root was subjected to a second steaming treatment for 8 hours at a temperature of 15° C. and a humidity of 40%. Next, the second steam-treated Korean ginseng root was placed in a freeze dryer and secondarily dried at -40°C for 12 hours. Then, the second dried ginseng root was extracted in 80% ethanol at 40° C. for 24 hours, and then filtered under reduced pressure. Next, the filtrate was concentrated under reduced pressure to prepare a high ginseng extract.

The Korean ginseng extract according to the present invention contains Oxymatrine as an active ingredient, and Oxymatrine has the structure of Formula 1 below.

The content of oximarine can be increased by steaming twice, which is a pre-treatment process, before extraction of Korean ginseng roots.

The high ginseng extract prepared using the pre-treated old ginseng root contains oximarine in an amount of 0.25 to 0.41% by weight. When the steaming is performed less than two times, the content of oxymatrine in the high ginseng extract becomes less than 0.25% by weight, so that the demodex removal effect is lowered. Rather, a small amount was lost.

The organic solvent used in this invention may be selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate, chloroform, and a mixed solvent of these organic solvents and water, and preferably 80% ethanol is used. At this time, the extraction temperature is preferably 10 ~ 80 ℃, can be extracted for 3 to 24 hours. If the extraction temperature and extraction time are out of the range, extraction efficiency may decrease or a change in components may occur.

The composition of the present invention, 0.001 to 15% by weight of the ginsenoside complex; 0.01 to 10% by weight of high ginseng extract; 20-40 wt% of surfactant; 30-70% by weight of purified water; 0.001 to 10% by weight of ceramide; 0.01-5% by weight of a moisturizer; 0.01 to 2% by weight of a preservative; and 0.5 to 3% by weight of fragrance.

The composition of the present invention preferably contains the ginsenoside complex in an amount of 0.003 to 10% by weight based on the total weight of the cosmetic composition. If the content of the ginsenoside complex is less than 0.001% by weight, the efficacy and effect of the component are weak, and if it exceeds 15% by weight, there is a problem with the formulation and feeling of use.

In the present invention, a high ginseng extract containing oximarine as an active ingredient is used as a demodex remover. The ginseng extract contains the alkaloid oxymatrine, and inhibits the activity of 5α-reductase type I and type II associated with hair loss, thereby showing an effect of alleviating hair loss symptoms. In addition, since oximarine is selectively toxic to pests without being toxic to the human body, it is widely used in cultivation as an eco-friendly control agent that can replace conventional chemically synthesized insecticides.

It is preferable that the composition of the present invention contains the ginseng extract in an amount of 0.01 to 10% by weight based on the total weight. If the content of ginseng extract is less than 0.01% by weight, there is a problem in that the effect of controlling Demodex is lowered, and if it exceeds 10% by weight, problems may occur in formulation and feeling.

The surfactant is a component that is essentially added for washing hair and scalp.

As the surfactant, lauryl glucoside, potassium cocoate, cocoglucoside, decyl glucoside, sodium cocoyl appleamino acid, potassium cocoyl glycy It may be selected from the group consisting of nate (Potassium cocoyl glycinate) or a mixture of two or more thereof.

Lauryl glucoside is a substance extracted from coconut oil and sugar, and it is used as a natural cleansing ingredient because it is eco-friendly because it biodegrades quickly and is very gentle to the skin.

Potassium cocoate is an anionic surfactant obtained by adding potassium (potassium) to coconut oil. It is known to have excellent cleaning power and is a naturally derived ingredient that does not irritate the skin mucous membrane and has excellent moisturizing effect after washing.

Cocoglucoside is a nonionic surfactant of natural ingredients derived from corn, coconut, and palm kernel oil as a condensation reaction of coconut alcohol and glucose. It is a light yellow liquid, water-soluble substance. The plant extract is known for its excellent cleansing and moisturizing effects.

Decyl glucoside is a substance obtained by the reaction of glucose extracted from corn starch and fatty alcohol extracted from vegetable oils such as coconut.

Sodium Cocoyl Apple Amino Acid is a sodium salt obtained by compressing an amino acid from apple juice and coconut acid chloride. As an anionic surfactant, it is a component extracted from apples, and it is excellent in foaming, and abundant foam is formed without adding a separate chemical surfactant. Chemical surfactants do not naturally decompose and return to clear water through chemical treatment, whereas sodium cocoyl apple amino acid is decomposed in nature, so it is known as an environmentally friendly component.

Potassium cocoyl glycinate is a vegetable amino acid-based anionic surfactant derived from coconut fruit, and is a component in which coconut oil fatty acids and glycine are synthesized. Eco-friendly cleaning ingredients with excellent biodegradability have excellent cleaning power and provide a refreshing feeling.

The surfactant may be used in an amount within the range of 20 to 40% by weight based on the total weight of the composition according to the present invention.

Ceramide is a substance belonging to lipids, and it is the most important component among the oils and fats contained in the keratin of the skin. is known

The preservative according to the present invention, in addition to the preservative components contained in general cosmetic compositions such as phenoxyethanol, 1,2-hexanediol, etc. may include more.

Scoria extract water contains a large amount of ionized minerals, which can improve stability by forming ionic bonds with oxygen of oximarine. Therefore, oxymatrine, a component derived from ginseng extract, is contained in the cosmetic composition for preventing hair loss and prevents the molecule from being decomposed during storage, so that the content of oxymatrin is maintained until the consumer uses it, thereby maintaining the demodex removal effect confirmed before distribution as a product. can indicate

For the scoria extract, 0.5 to 5 parts by weight of scoria is added to 100 parts by weight of purified water, aged at 45 to 60° C. for 12 hours, desalted to a salt concentration of 0.85 to 0.90%, and filtered.

When the oximarine is stored using ordinary distilled or purified water, the decomposition rate of oximarine is up to 80% after a certain period of time has elapsed, so there is a problem that the effect of the composition may disappear during the distribution process.

The salt concentration of Scoria extract water is desalted to 0.85 to 0.90%, which is the physiological salt concentration in the body, in terms of scalp protection and improvement.

The fragrance may be composed of synthetic fragrances or natural fragrances that are widely used or known in cosmetic compositions.

A shampoo composition for preventing hair loss was prepared in the composition ratio shown in Table 3 below using the wild ginseng cultured root extract, wild ginseng extract, scoria extract, lauryl glucoside (naturally derived surfactant) and other auxiliary ingredients according to the present invention. At this time, the ginsenoside complex contains 0.001 to 15% by weight of a complex in which ginsenosides Rg3, Rg4 and Rg6 are each mixed in a weight ratio of 1-3: 1-3: 1-3 as an active ingredient.,

Hereinafter, the present invention will be described in more detail through the accompanying Examples, Comparative Examples and Experimental Examples. However, these embodiments are merely examples for easily explaining the content and scope of the technical spirit of the present invention, and thereby the technical scope of the present invention is not limited or changed. It will be apparent to those skilled in the art that various modifications and variations are possible.

(Examples 1 to 6) Preparation of ginsenoside complex

Ginsenosides Rg3, Rg4, and Rg6 obtained by purifying and separating the wild ginseng cultured root extract prepared through the pretreatment process are mixed in a weight ratio of 1-3: 1-3: 1-3, respectively, and ginsenosides of the same weight A composite was prepared.

Ginsenosides Rg3, Rg4 and Rg6 were purchased from Chromadex and mixed in the ratio shown in Table 1 below to prepare a ginsenoside complex of the same weight.

(Comparative Example 1) Preparation of red ginseng extract

After the dried red ginseng was washed with purified water, dried and closed into small pieces, it was added to 1 L of 80% ethanol, extracted twice at 65° C. for 8 hours, and filtered under reduced pressure to prepare a red ginseng extract.

(Comparative Examples 2-7) Preparation of ginsenoside mixture

Ginsenosides Rg3, Rg4, and Rg6 were mixed in the ratio shown in Table 2 below to prepare a ginsenoside mixture of the same weight.

(Formulation Example) Preparation of shampoo containing ginsenoside complex and ginseng extract

Shampoos containing the ginsenoside complex and high ginseng extract of the above Examples, the red ginseng extract of Comparative Example 1 and the ginsenoside mixture of Comparative Examples 2-7 were prepared in a conventional manner according to the compositions of Tables 3 and 4 below. .

(Experimental Example 1) Hair growth test

Using the shampoo containing the complex consisting of the ginsenosides Rg3, Rg4 and Rg6 of Examples 1 to 6, the red ginseng extract of Comparative Example 1, and the ginsenoside mixture of Comparative Examples 2 to 7, respectively, was evaluated in the following manner did.

In order to confirm the hair growth promoting effect of the shampoo composition for alleviating hair loss symptoms according to the present invention, the above shampoo compositions (Examples 1 to 6, Comparative Examples 1 to 7) were prepared, and a total of 39 subjects in their 20s and 50s were targeted. An evaluation experiment was performed on the effect of alleviating hair loss symptoms and promoting hair growth. The subjects were divided into 13 groups (Examples 1 to 6, Comparative Examples 1 to 7) of 3 people each, and they were used to wash the scalp in the evening once a day for 14 days. After use, the degree of hair growth was checked according to the opinions of the subjects, and the criteria for the determination are shown in Table 5, and the results are shown in Tables 6 and 7.

As can be seen in Tables 6 and 7, the complex consisting of the ginsenosides Rg3, Rg4, and Rg6 of the Examples is superior to the red ginseng extract of Comparative Example 1 or a mixture of some ginsenosides of Comparative Examples 2-7. It has a stimulating effect. In particular, the compound of Examples 1 to 4, in which the mixing ratio of ginsenoside is 1-3: 1-3: 1-3, showed excellent hair growth, and the most excellent effect in Example 3 having a mixing ratio of 3: 1: 1 represents Through this, it is possible to confirm the effect of relieving hair loss symptoms and promoting hair growth of the complex composed of the ginsenosides Rg3, Rg4, and Rg6 of the present invention.

(Experimental Example 2) Clinical trial

In order to clinically confirm the hair loss symptom alleviation function of the specific ginsenoside Rg3, Rg4 and Rg6 complexes of Example 3, a clinical test was conducted using the shampoo composition containing the ginsenoside complex of Example 3. As a control example, a shampoo composition that does not contain the ginsenoside complex was used.

In order to confirm the efficacy of the function for alleviating hair loss symptoms, the shampoo containing the ginsenoside complex of Example 3 was divided into 2 groups for a total of 46 male and female adult males and females between the ages of 18 and 54 with hair loss symptoms, and After using the comparative shampoo for 24 weeks at the designated test site, scalp measurements were taken before, 8 weeks, 16 weeks, and 24 weeks after use. Hair density measurement, hair photography, visual evaluation, and questionnaire evaluation were performed.

In order to determine the significance of the changes in the scalp measurements before and after use, it was verified using SPSS 23.0, a statistical analysis program. The statistical significance was confirmed when the significance probability was p<0.05 at the 95% confidence interval.

Hair Density Measurement (Phototricogram)

To measure the hair density, the area to be evaluated was first designated, and the hair was shaved to a certain area (about 1 cm2). For scalp photography, the test site was taken before and 24 weeks after use of the test product using Folliscope 4.0, and the total number of hairs in a 1cm2 circle of the captured image was analyzed and used as hair density data.

As a result of measuring hair density after using the product, in the case of the formulation containing Example 3, there was a statistically significant increase after 24 weeks of use compared to before use (p<0.05). The results are shown in Table 8.

Hair Visual Assessment

Subjects photographed the entire hair at a 45-degree angle under the same conditions every time, and two experts visually evaluated the hair condition according to the criteria. If there was a difference in the evaluation of two people, the lower level was selected. The visual evaluation scores according to the degree of hair distribution are as follows.

(very good +3, good +2, slightly better +1, no change 0, very bad -3, worse -2, slightly worse -1)

As a result of the expert visual evaluation, the visual evaluation score showed a statistically significant difference after 24 weeks of use between the groups using the formulation containing Example 3 and the group using the formulation of the control example. (p<0.05) The results are shown in Table 9.

As such, it can be seen that even when the shampoo of Example 3 containing a trace amount (0.1%) of the ginsenoside complex of the present invention is used, a significant functional effect of alleviating hair loss symptoms is exhibited.

safety assessment

In order to confirm the safety of the product, the safety of the product was evaluated by confirming both subjective and objective stimulation through questionnaires and expert observation.

- Subjective irritation (subject evaluation): itching, stinging, burning, stiffness, tingling

- Objective irritation (expert evaluation): erythema, edema, papule, scale

The results are shown in Table 10.

(Stage = 0: None, 1: Weak, 2: Medium, 3: Strong)

As confirmed in Table 9, both formulations were confirmed to be safe.

(Experimental Example 3) Demodex removal effect of ginseng extract

In order to check whether the demodex removal effect is exhibited when the ginseng extract according to the present invention is included in the shampoo composition for preventing hair loss, a shampoo composition for alleviating hair loss symptoms (Examples 1 to 4, Comparative Example 1) was prepared and the Demodex survival inhibitory effect was shown below. was confirmed in the same way.

After attaching an electron microscope to a CCD camera, it was connected to a computer, and using a blade, Demodex was obtained from hair follicles. Demodex was placed on a slide glass and the same amount of shampoo composition for alleviating hair loss symptoms was added, and then the cover glass was erected at a right angle and covered, and the movement of the demodex's body and legs was observed for 10 minutes. After 10 minutes, the change in the movement of Demodex was evaluated as '0: no movement, 1: slow, 2: no change', and the results are shown in Table 11 below.

As shown in the above results, in Comparative Example 1 to which the ginseng extract was not added, it was observed that the movement of Demodex was not changed, and it was determined that there was no Demodex removal effect. On the other hand, in Examples 1-4 to which the high ginseng extract was added, there was no movement of the Demodex and it was determined that they were killed. Therefore, in the present invention, the shampoo composition for preventing hair loss including the ginseng extract exhibits an effect of removing Demodex.

(Experimental Example 4) Oxymatrin decomposition rate according to the type of filtered water

In order to check whether the storage stability of oxymatrine is increased in the shampoo composition for preventing hair loss containing Scoria extract according to the present invention, a shampoo composition for preventing hair loss (Examples 3 to 4, Comparative Examples 2 to 4) is prepared, and the storage period Oxymatrin degradation rate was measured according to

Each shampoo composition for preventing hair loss was packaged in three identical containers and stored in a thermostat at 25° C. for 12 weeks, and the average content of oxymatrin before and after 12 weeks was compared. The content of oximarine was analyzed using liquid chromatography (HPLC). The results are shown in Table 12 below.

As shown in the above results, it was confirmed that in Comparative Examples 2 to 4, the degradation rate of oxymatrin was up to 80%, and storage was not easy due to the unstable oximarin molecule. On the other hand, in Examples 3 and 4 containing Scoria extract, the decomposition rate of oximarine was measured to be up to 2%, confirming that the stability was significantly increased. Therefore, the shampoo composition for preventing hair loss including the scoria extract of the present invention can increase the storage stability of oxymatrine, which exhibits an effect of removing Demodex.

Although the above has been described with reference to the embodiments, those skilled in the art will understand that various modifications and changes can be made to the present invention without departing from the spirit and scope of the present invention as set forth in the following claims. will be able

Claims (2)

0.001 to 15% by weight of the ginsenoside complex; 0.01 to 10% by weight of high ginseng extract; 20-40 wt% of surfactant; 30-70% by weight of purified water; 0.01-5% by weight of a moisturizer; 0.001 to 10% by weight of ceramide; 0.01 to 2% by weight of a preservative; and 0.5 to 3% by weight of fragrance,
The ginsenoside complex is obtained by obtaining ginsenoside Rg3, Rg4 and Rg6 from any one of wild ginseng cultured root extract, red ginseng extract, ginseng extract or black ginseng extract, each of which is subjected to high-pressure enzymatic decomposition after steaming twice. Senoside Rg3, Rg4 and Rg6, respectively 1-3: 1-3: A cosmetic composition for alleviating hair loss symptoms, characterized in that it is made by mixing in a weight ratio of 1-3.
The method of claim 1,
The high ginseng extract is characterized in that it contains oximarine in an amount of 0.25 to 0.41 wt% by steaming twice before extraction,
The preservative is characterized in that it contains scoria extract in order to increase the stability of oximarine derived from ginseng extract in water, and the scoria extract is 45-60 parts by weight of scoria with respect to 100 parts by weight of purified water. Characterized in that it is aged at ℃ for 12 hours, desalted to a salt concentration of 0.85 to 0.90% and filtered,
A cosmetic composition for alleviating hair loss symptoms, characterized in that it removes Demodex and improves the stability of oxymatrine.