CN107410365A - A kind of trichodermaharzianum microbial inoculum and its preparation method and application - Google Patents
A kind of trichodermaharzianum microbial inoculum and its preparation method and application Download PDFInfo
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- CN107410365A CN107410365A CN201710429260.4A CN201710429260A CN107410365A CN 107410365 A CN107410365 A CN 107410365A CN 201710429260 A CN201710429260 A CN 201710429260A CN 107410365 A CN107410365 A CN 107410365A
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- CN
- China
- Prior art keywords
- trichodermaharzianum
- microbial inoculum
- bacterium powder
- amino acid
- soil
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
- C09K17/18—Prepolymers; Macromolecular compounds
- C09K17/32—Prepolymers; Macromolecular compounds of natural origin, e.g. cellulosic materials
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
Abstract
The invention discloses a kind of trichodermaharzianum microbial inoculum and its preparation method and application, wherein, the microbial inoculum includes：Trichodermaharzianum bacterium powder, amino acid and potassium fulvate, trichodermaharzianum bacterium powder are obtained by the trichodermaharzianum that deposit number is CGMCC No.13771 in wheat bran medium top fermentation.The present invention is advantageous in that：(1) trichodermaharzianum microbial inoculum of the invention can not only effectively prevent and treat the soil-borne diseases such as Strawberry Root Rot, cucumber blight dis-ease, moreover it is possible to improved soil, promote plant growth, have very positive using effect；(2) trichodermaharzianum microbial inoculum spore content of the invention is higher, it is possible to reduce mu dosage, it is cost-effective；(3) trichodermaharzianum microbial inoculum application method of the invention is various, can save manpower and use cost according to different situations reasonable employment.
Description
Technical field
The present invention relates to a kind of microbial bacterial agent and its preparation method and application, and in particular to a kind of trichodermaharzianum microbial inoculum and
Its preparation method and application, belong to field of agricultural microbial technology.
Background technology
Soil-borne disease occurrence regularity is complicated, its spread in china is fast, harm is serious, and production loss is larger, often results in soil ecology
Environmental degradation, it can occur in the anniversary.
Soil-borne disease is also to cause one of main reason of continuous cropping obstacle, and continuous cropping provides for the amount reproduction of pathogen
Favourable places, have aggravated the generation of soil-borne disease.
Soil-borne disease species is more, common are Cruciferae sclerotiniose, soft rot, the samping off such as solanberry, melon, vertical withered
Disease, epidemic disease, root rot, droop and verticillium wilt, tomato, cucumber and paprike bacterial wilt, root knot nematode disease etc..
In recent years, due to the continuous expansion of continuous cropping area, medication not science, production in crop-planting species it is single, highly dense
Spend sequential cropping cultivation and lack the influence of the cultivation steps such as effective crop rotation, cause soil-borne disease developmenting spread speed, resistance
Enhancing, crop death rate are continuously increased, and so as to cause crop Severe Reduction, or even total crop failure, quality is reduced, had a strong impact on
Production estimation.
The content of the invention
To solve the deficiencies in the prior art, it is an object of the invention to provide one kind is simple to operate, field planting power is strong, will not produce
The raw resistance to the action of a drug, it can effectively prevent and treat trichodermaharzianum microbial inoculum of soil-borne disease and preparation method thereof.
In order to realize above-mentioned target, the present invention adopts the following technical scheme that：
A kind of trichodermaharzianum microbial inoculum, it is characterised in that including：Trichodermaharzianum bacterium powder, amino acid and potassium fulvate, wherein,
Foregoing trichodermaharzianum bacterium powder is CGMCC No.13771 by deposit number (Latin literary fame is referred to as Trichoderma koningii)
Trichodermaharzianum obtained in wheat bran medium top fermentation.
Foregoing trichodermaharzianum microbial inoculum, it is characterised in that in foregoing trichodermaharzianum bacterium powder, the spore content of trichodermaharzianum
>=100 hundred million/gram.
Foregoing trichodermaharzianum microbial inoculum, it is characterised in that foregoing trichodermaharzianum bacterium powder, amino acid and potassium fulvate are according to 5：
1-3：0.5-1.5 mass ratio mixing.
Foregoing trichodermaharzianum microbial inoculum, it is characterised in that foregoing trichodermaharzianum bacterium powder, amino acid and potassium fulvate are according to 5：
2：1 mass ratio mixing.
Foregoing trichodermaharzianum microbial inoculum, it is characterised in that preparation method is as follows：
Step1：The trichodermaharzianum that deposit number is CGMCC No.13771 is seeded on PDA plate culture medium, 25-28
DEG C light culture 5-10d, using cultured flat board as original seed；
Step2：The cultured original seeds of Step1 are seeded in PDA liquid medium, 25-28 DEG C, 121rpm light cultures 3-
5d, using the liquid fermented as seed liquor；
Step3：By wheat bran and water according to 70：25-35 mass ratio is mixed, and fermentation medium is used as after sterilizing；
Step4：The cultured seed liquors of Step2 are added in fermentation medium made from Step3,25-28 DEG C of standing
Light culture, when spore content >=20,000,000,000/gram when stop fermentation；
Step5：Zymotic fluid made from Step4 is dried, crush, sieving, obtain spore content >=10,000,000,000/gram it is healthy and free from worry
Trichoderma bacterium powder；
Step6：Trichodermaharzianum bacterium powder made from Step5 is mixed with amino acid and potassium fulvate.
The present invention is advantageous in that：
(1) trichodermaharzianum can be occupied with fast-growth progress ecological niche, while produced a variety of secondary metabolites and suppressed disease
Opportunistic pathogen grows, experiment proves that, trichodermaharzianum microbial inoculum of the invention can effectively prevent and treat the soil such as Strawberry Root Rot, cucumber blight dis-ease
Disease is passed, there is very positive using effect；
(2) trichodermaharzianum microbial inoculum of the invention, spore content are higher, it is possible to reduce mu dosage, it is cost-effective；
(3) trichodermaharzianum microbial inoculum of the invention, can drip irrigation, Ke Chongshi, can be used in mixed way with base fertilizer, application method is various,
Manpower and use cost can be saved according to different situations reasonable employment；
(4) trichodermaharzianum microbial inoculum of the invention, can produce a variety of secondary metabolites, so as to improved soil, promote
Enter plant growth；
(5) trichodermaharzianum microbial inoculum of the invention, its technique is simple, is adapted to factorial praluction.
Embodiment
Make specific introduce to the present invention below in conjunction with specific embodiment.
Part I：Obtain trichodermaharzianum
1st, isolated strains are screened
The soil sample gathered from greenhouse is diluted on PDA plate and is separately cultured, picking grows faster bacterium colony.
2nd, bacterial strain is identified
Molecular Detection is carried out using ITS sequence, testing result shows that isolated bacterial strain is trichodermaharzianum.
3rd, bacterial strain preservation
We will screen isolated trichodermaharzianum and were preserved in Chinese microorganism strain preservation on 03 31st, 2017
Administration committee's common micro-organisms center (Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), deposit number CGMCC
No.13771。
Part II：Prepare trichodermaharzianum microbial inoculum
1st, original seed is cultivated
The trichodermaharzianum that deposit number is CGMCC No.13771 is seeded on PDA plate culture medium, 27 DEG C of light cultures
7d, using cultured flat board as original seed.
2nd, seed liquor is cultivated
It cultured original seed will above be seeded in PDA liquid medium, 27 DEG C, 121rpm light culture 4d, will ferment
Liquid as seed liquor.
3rd, fermentation medium is made
By wheat bran and water according to 7：3 mass ratio is well mixed, and 121 DEG C of sterilizing 30min, the fermentation as trichodermaharzianum is trained
Base is supported, it is stand-by.
4th, ferment
It cultured seed liquor will above be added in above obtained fermentation medium, and 27 DEG C of standing light cultures, treat spore
Sub- content >=20,000,000,000/gram when stop fermentation.
5th, bacterium powder is made
Will above obtained zymotic fluid drying, crush, cross 100 mesh sieve, that is, obtain spore content >=10,000,000,000/gram health
Peaceful trichoderma bacterium powder.
6th, batch mixing
Will above obtained trichodermaharzianum bacterium powder be mixed with amino acid and potassium fulvate, trichodermaharzianum bacterium powder, amino acid and
Mass ratio between potassium fulvate three is 5：1-3：0.5-1.5, produce trichodermaharzianum microbial inoculum.
In the present embodiment, the mass ratio between trichodermaharzianum bacterium powder, amino acid and potassium fulvate three is 5：2：1.
Part III：Study preventive and therapeutic effect of the trichodermaharzianum microbial inoculum to soil-borne disease
1st, preventive effect of the trichodermaharzianum microbial inoculum to Strawberry Root Rot
Certain higher greenhouse of Strawberry Root Rot morbidity in Qingdao, chooses two mu of ground, and wherein 0.07 hectare applies the health of the present invention
Peaceful trichoderma 1kg (as experimental group), application process is to carry out punching to root soil to apply, and 0.07 hectare is without using any place in addition
Reason is (as a control group).
Comparative result is as follows：
| Fall ill rate | Preventive effect | |
| Experimental group | 19% | 70.8% |
| Control group | 65% | —— |
2nd, preventive effect of the trichodermaharzianum microbial inoculum to cucumber blight dis-ease
Certain higher greenhouse of cucumber blight dis-ease morbidity in Shandong Changle, chooses two mu of ground, and wherein 0.07 hectare applies the present invention
Trichodermaharzianum microbial inoculum 1kg (as experimental group), application process is to carry out punching to root soil to apply, in addition 0.07 hectare without using appoint
Manage (as a control group) where.
Comparative result is as follows：
| Fall ill rate | Preventive effect | |
| Experimental group | 14% | 83.1% |
| Control group | 83% | —— |
As can be seen here：The paecilomyces varioti microbial inoculum of the present invention can effectively prevent and treat nematode.
Trichodermaharzianum can carry out ecological niche with fast-growth and occupy, while produces a variety of secondary metabolites and suppress cause of disease
The growth of bacterium, so the trichodermaharzianum microbial inoculum of the present invention can effectively prevent and treat the soil-borne diseases such as Strawberry Root Rot, cucumber blight dis-ease.
Part IV：Study facilitation of the trichodermaharzianum microbial inoculum to plant growth
It is well mixed with the trichodermaharzianum microbial inoculum of the present invention with water, carrying out punching to the pepper seedling of 0.07 hectare applies, and investigates peppery
The growing way and yield of green pepper seedling.
If control group, the pepper seedling of 0.07 hectare is poured with clear water, investigates the growing way and yield of pepper seedling.
It is as follows through investigation, the growing way and yield of pepper seedling：
| Experimental group (average value) | Control group (average value) | |
| Plant height | 58cm | 51cm |
| Yield | 2600kg | 2400kg |
As can be seen here：The trichodermaharzianum microbial inoculum of the present invention also has while soil-borne disease is prevented and treated promotes plant growth
Effect.
Trichodermaharzianum can produce a variety of secondary metabolites, so as to improved soil, promote plant growth.
It should be noted that the invention is not limited in any way for above-described embodiment, it is all to use equivalent substitution or equivalent change
The technical scheme that the mode changed is obtained, all falls within protection scope of the present invention.
Claims (7)
- A kind of 1. trichodermaharzianum microbial inoculum, it is characterised in that including：Trichodermaharzianum bacterium powder, amino acid and potassium fulvate, wherein, institute Trichodermaharzianum bacterium powder is stated to be obtained in wheat bran medium top fermentation by the trichodermaharzianum that deposit number is CGMCC No.13771.
- 2. trichodermaharzianum microbial inoculum according to claim 1, it is characterised in that in the trichodermaharzianum bacterium powder, healthy and free from worry wood Mould spore content >=10,000,000,000/gram.
- 3. trichodermaharzianum microbial inoculum according to claim 2, it is characterised in that trichodermaharzianum bacterium powder, amino acid and the Huang Rotten sour potassium is according to 5：1-3：0.5-1.5 mass ratio mixing.
- 4. trichodermaharzianum microbial inoculum according to claim 3, it is characterised in that trichodermaharzianum bacterium powder, amino acid and the Huang Rotten sour potassium is according to 5：2：1 mass ratio mixing.
- 5. the trichodermaharzianum microbial inoculum according to Claims 1-4 any one, it is characterised in that preparation method is as follows：Step1：The trichodermaharzianum that deposit number is CGMCC No.13771 is seeded on PDA plate culture medium, 25-28 DEG C dark 5-10d is cultivated, using cultured flat board as original seed；Step2：The cultured original seeds of Step1 are seeded in PDA liquid medium, 25-28 DEG C, 121rpm light culture 3-5d, Using the liquid fermented as seed liquor；Step3：By wheat bran and water according to 70：25-35 mass ratio is mixed, and fermentation medium is used as after sterilizing；Step4：The cultured seed liquors of Step2 are added in fermentation medium made from Step3,25-28 DEG C stands dark training Support, when spore content >=20,000,000,000/gram when stop fermentation；Step5：Zymotic fluid made from Step4 is dried, crush, sieving, obtain spore content >=10,000,000,000/gram trichodermaharzianum Bacterium powder；Step6：Trichodermaharzianum bacterium powder made from Step5 is mixed with amino acid and potassium fulvate.
- 6. application of the trichodermaharzianum microbial inoculum in terms of soil-borne disease is prevented and treated described in claim 5.
- 7. application of the trichodermaharzianum microbial inoculum in terms of improved soil described in claim 5.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111393195A (en) * | 2020-04-13 | 2020-07-10 | 江苏小瓢虫生物科技有限公司 | Formula and preparation method of plant immune protein composite microbial inoculum |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102731209A (en) * | 2012-06-27 | 2012-10-17 | 河南农飞生物科技有限公司 | Amino acid humic acid organic and inorganic compound mixing fertilizer and production method thereof |
| CN104498369A (en) * | 2014-12-05 | 2015-04-08 | 中国农业科学院植物保护研究所 | Trichoderma koningii and bacterial agent containing same and application thereof in prevention of cylindrocarpon destructans |
-
2017
- 2017-06-08 CN CN201710429260.4A patent/CN107410365A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102731209A (en) * | 2012-06-27 | 2012-10-17 | 河南农飞生物科技有限公司 | Amino acid humic acid organic and inorganic compound mixing fertilizer and production method thereof |
| CN104498369A (en) * | 2014-12-05 | 2015-04-08 | 中国农业科学院植物保护研究所 | Trichoderma koningii and bacterial agent containing same and application thereof in prevention of cylindrocarpon destructans |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111393195A (en) * | 2020-04-13 | 2020-07-10 | 江苏小瓢虫生物科技有限公司 | Formula and preparation method of plant immune protein composite microbial inoculum |
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Application publication date: 20171201 |