CN107400008A - A kind of process of preparing of edible and medical fungi activity improver - Google Patents
A kind of process of preparing of edible and medical fungi activity improver Download PDFInfo
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- CN107400008A CN107400008A CN201710610944.4A CN201710610944A CN107400008A CN 107400008 A CN107400008 A CN 107400008A CN 201710610944 A CN201710610944 A CN 201710610944A CN 107400008 A CN107400008 A CN 107400008A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention belongs to agricultural fertilizer production technical field, specifically provides a kind of process of preparing of edible and medical fungi activity improver, and step is：The first step, weigh humic acid potassium, urea, amino acid, potassium dihydrogen phosphate and boric acid and mix as the first batch mixing；Second step, weigh magnesium sulfate, zinc sulfate, ferrous sulfate, copper sulphate, manganese chloride and disodium ethylene diamine tetraacetate and mix as the second batch mixing, into the second batch mixing plus water stirring reaction；3rd step, the first batch mixing is added in the reaction solution of second step, add water to react, adjust pH, add high energy oligosaccharides, dried after stirring reaction, crush and produce.The activity improver obtained by the process of preparing of the present invention is rich in enzymatic activity, strengthens degraded, absorption, conveying and the ability for storing nutriment of white fungus mycelia and incense ashes mycelia in matrix, promotes mycelia rejuvenation and regeneration, volume increase 10% -20%.After white fungus matrix is applied, the polyoses content of fructification improves 15% -30%, and mushroom quality greatly improves.
Description
Technical field
The invention belongs to agricultural fertilizer production technical field, it is related to the production technology of edible and medical fungi, more particularly to white fungus especially
Production technology, and in particular to a kind of process of preparing of edible and medical fungi activity improver.
Background technology
Edible and medical fungi is that a kind of plantation mushroom include edible mushroom and medicinal fungus, some while have edible and medicinal bacterium
Class also turns into edible and medical fungi in the narrow sense.Edible and medical fungi has had extensive artificial growth culture in human history and gone through
History, have become one of indispensable life raw material of the mankind.The production technical field of edible and medical fungi at present, it is existing especially
Two prominent problems are that the yield of edible and medical fungi needs to be further improved, and the quality of edible and medical fungi remains to be further improved.
Therefore, substantial amounts of research has been done in industry, but it is poor to take effect.
In edible and medical fungi, the history of life of white fungus is the most special, is embodied in two aspects：With yeast-like-conidium
Asexual generation, it is necessary to incense ashes bacterium association.White fungus is the heterothallic mushroom of quadripolarity, in fructification maturation, auricle surface
Many basidiospore are produced, budding forms yeast-like-conidium to basidiospore under appropriate conditions, then is sprouted by it into monokaryon bacterium
Silk, for two uninucleate hyphaes that can be affine by matter with forming nucleated mycelium, nucleated mycelium has a clamp connection, bacterium colony show it is spherical or
Fine hair bulk, if condition of culture uncomfortable (heated, immersion) or mycelia are injured, nucleated mycelium can form yeast-like-conidium.Ferment
Female shape conidium ellipse, monokaryon, carries out vegetative propagation, it can sprout to form double-core under optimum conditions in a manner of budding
Mycelia.White fungus completes the mistake of its history of life in, it is necessary to the assistance of another fungi, i.e. incense ashes bacterium.Incense ashes mycelial growth is fast
Speed, initial stage, white, rear gradual change canescence, there is the black scar of carbonaceous, and culture medium is changed into dark brown sometimes.White fungus degraded is wooden
The ability of cellulose is weak, it is necessary to which incense ashes bacterium association, assists white fungus degraded to utilize matrix.After white fungus mycelia raised growth, accumulation battalion
Support, reach physiological maturity, be split into " white hair group " in stromal surface and colloidization forms ear base.Ear base is in suitable environmental condition
Under, gradually development, differentiates auricle, forms children's reality layer in the outer surface of auricle, produces basidiospore.
White fungus growth is divided into vegetative growth phase and generative growth phase, also cries mycelium stage and produce agaric stage.Mycelia
The body stage is that the nutriment in culture medium is degraded, absorbed, is stored in mycelium, and the produce agaric stage is mycelial battalion
Foster material is conveyed to fructification, grows sporophore growth.Therefore, determine white fungus yield and quality how, except with white fungus
Original seed, growing nutrient key element and growing environment are relevant outer, also with the nutriment of culture medium can fully degraded absorbed with
And can mycelium completely be conveyed to nutriment fructification very big relation.The nutrient substance of existing white fungus is universal
It can not meet that white fungus grows demand very well.
Analyzed based on above state of the art, required for how preparing in a kind of edible and medical fungi incubation
Improver, it is technical problem urgently to be resolved hurrily to improve the yield and quality of edible and medical fungi especially white fungus generally existing.
The content of the invention
In order to solve above mentioned problem existing for prior art, the present invention, which provides one kind, can improve edible and medical fungi especially
The process of preparing of white fungus quality or the lifting edible and medical fungi especially edible and medical fungi activity improver of white fungus yield.
According to the process of preparing of the edible and medical fungi activity improver of the embodiment of the present invention, including such as
Lower step：
The first step, humic acid potassium, urea, amino acid, potassium dihydrogen phosphate and boric acid are weighed, first is obtained after well mixed
Batch mixing is standby；
Second step, magnesium sulfate, zinc sulfate, ferrous sulfate, copper sulphate, manganese chloride and disodium ethylene diamine tetraacetate are weighed, is added
Enter that reactor is well mixed to obtain the second batch mixing, water is added into reactor, intermediate reaction liquid is obtained after stirring reaction；
3rd step, will first batch mixing add reactor the intermediate reaction liquid in, be then kept stirring for continue to
Water is added in reactor, potassium hydroxide regulation pH to 6.5~7.5 is then added, finally adds high energy oligosaccharides, stirring reaction
After dry, crush and produce.
According to the process of preparing of the edible and medical fungi activity improver of the embodiment of the present invention, wherein excellent
Choosing, magnesium sulfate in second step, zinc sulfate, ferrous sulfate, copper sulphate and manganese chloride total material amount and ethylenediamine tetra-acetic acid
The ratio (namely mol ratio) of the amount of the material of disodium is 1:1.
According to the process of preparing of the edible and medical fungi activity improver of the embodiment of the present invention, wherein excellent
Choosing, in second step, the reaction time of stirring reaction is 120~150min, and the reaction temperature of stirring reaction is 60~80 DEG C.
According to the preparation technology of the edible and medical fungi activity improver of the embodiment of the present invention, the present invention provides
The preparation technology of foregoing edible and medical fungi activity improver of the invention, wherein preferably, in the 3rd step, keeping in reactor
Temperature carries out the addition of the first batch mixing under the conditions of 60~80 DEG C.
According to the process of preparing of the edible and medical fungi activity improver of the embodiment of the present invention, wherein excellent
Choosing, high energy oligosaccharides is feed grade high energy oligosaccharides, is cornstarch extract, can directly be bought via in industry, can also
Individually produce, specific reparation technology when individually producing is：The smart starch milk that protein content is less than 0.5% is chosen, through adjusting breast
It is 5.5-6.0 to make pH, adds high temperature resistant enzyme, through high-temperature injection twice, wherein after spraying for the first time, maintain 90 at 95 DEG C~
120 minutes, it was 120~135 DEG C to spray temperature for the second time, 3~5 minutes time.After cooling, invertase is added, through certain time
Conversion reaches defined purity, then filtered, concentration, decrease temperature crystalline, and it is high energy oligosaccharides to crush, dry.Pass through the present invention
The high energy oligosaccharides that the reparation technology of offer is obtained is more containing isomalto-oligosaccharide, manna oligosacchride, glucan and biotin H etc.
Kind is easy to the active material of edible and medical fungi growth.Preferably, humic acid potassium is weathered coal extract.
According to the process of preparing of the edible and medical fungi activity improver of the embodiment of the present invention, wherein excellent
Choosing, the edible and medical fungi that the 3rd step obtains after crushing is respectively with the weight accounting of each nutritional ingredient in activity improver：Corruption is planted
Acid >=4%, wherein amino acid >=8%, N+P+K >=20%, N >=10%, Mg+Zn+Fe+Cu+Mn+B >=4%, active factors
(high energy oligosaccharides) >=5%, more than 100 mesh and uniformly, moisture is less than 0.5% to pulvis mesh number, pH value control 6.5~
Between 7.5.
According to the process of preparing of the edible and medical fungi activity improver of the embodiment of the present invention, wherein excellent
Choosing, the weight of the water added in second step into reactor is 0.5 times of the second batch mixing gross weight；To reaction in 3rd step
The weight of the water added in kettle is 0.5 times of the first batch mixing gross weight.
According to the process of preparing of the edible and medical fungi activity improver of the embodiment of the present invention, wherein excellent
Choosing, the parts by weight usage ratio of the raw material respectively used is respectively：8~13 parts of humic acid potassium, 20~30 parts of urea, amino acid 8
~10 parts, 10~15 parts of magnesium sulfate, 10~15 parts of potassium dihydrogen phosphate, 2~3 parts of zinc sulfate, 2~3 parts of boric acid, ferrous sulfate 2~
3 parts, 2~3 parts of copper sulphate, 2~3 parts of manganese chloride, 3~5 parts of potassium hydroxide, 8~10 parts of disodium ethylene diamine tetraacetate, high energy it is few
5~9 parts of sugar.
According to the process of preparing of the edible and medical fungi activity improver of the embodiment of the present invention, wherein excellent
Choosing, the parts by weight usage ratio of the raw material respectively used is respectively：
According to the process of preparing of the edible and medical fungi activity improver of the embodiment of the present invention, wherein excellent
Choosing, the parts by weight usage ratio of the raw material respectively used is respectively：
According to the process of preparing of the edible and medical fungi activity improver of the embodiment of the present invention, wherein excellent
Choosing, the parts by weight usage ratio of the raw material respectively used is respectively：8 parts of humic acid potassium, 20 parts of urea, 8 parts of amino acid, sulfuric acid
10 parts of magnesium, 10 parts of potassium dihydrogen phosphate, 2 parts of zinc sulfate, 2 parts of boric acid, 2 parts of ferrous sulfate, 2 parts of copper sulphate, 2 parts of manganese chloride, hydrogen-oxygen
Change 3 parts of potassium, 8 parts of disodium ethylene diamine tetraacetate, 5 parts of high energy oligosaccharides.
According to the process of preparing of the edible and medical fungi activity improver of the embodiment of the present invention, wherein excellent
Choosing, the parts by weight usage ratio of the raw material respectively used is respectively：13 parts of humic acid potassium, 30 parts of urea, 0 part of amino acid/11, sulphur
Sour 15 parts of magnesium, 15 parts of potassium dihydrogen phosphate, 3 parts of zinc sulfate, 3 parts of boric acid, 3 parts of ferrous sulfate, 3 parts of copper sulphate, 3 parts of manganese chloride, hydrogen
5 parts of potassium oxide, 10 parts of disodium ethylene diamine tetraacetate, 9 parts of high energy oligosaccharides.
According to the process of preparing of the edible and medical fungi activity improver of the embodiment of the present invention, wherein excellent
Choosing, the parts by weight usage ratio of the raw material respectively used is respectively：8 parts of humic acid potassium, 30 parts of urea, 8 parts of amino acid, sulfuric acid
15 parts of magnesium, 10 parts of potassium dihydrogen phosphate, 3 parts of zinc sulfate, 2 parts of boric acid, 3 parts of ferrous sulfate, 2 parts of copper sulphate, 3 parts of manganese chloride, hydrogen-oxygen
Change 3 parts of potassium, 10 parts of disodium ethylene diamine tetraacetate, 5 parts of high energy oligosaccharides.
According to the process of preparing of the edible and medical fungi activity improver of the embodiment of the present invention, wherein excellent
Choosing, the parts by weight usage ratio of the raw material respectively used is respectively：13 parts of humic acid potassium, 20 parts of urea, 0 part of amino acid/11, sulphur
Sour 10 parts of magnesium, 15 parts of potassium dihydrogen phosphate, 2 parts of zinc sulfate, 3 parts of boric acid, 2 parts of ferrous sulfate, 3 parts of copper sulphate, 2 parts of manganese chloride, hydrogen
5 parts of potassium oxide, 8 parts of disodium ethylene diamine tetraacetate, 9 parts of high energy oligosaccharides.
According to the process of preparing of the edible and medical fungi activity improver of the embodiment of the present invention, wherein excellent
Choosing, the parts by weight usage ratio of the raw material respectively used is respectively：11 parts of humic acid potassium, 25 parts of urea, 9 parts of amino acid, sulfuric acid
12 parts of magnesium, 13 parts of potassium dihydrogen phosphate, 2.5 parts of zinc sulfate, 2.5 parts of boric acid, 2.5 parts of ferrous sulfate, 2.5 parts of copper sulphate, manganese chloride
2.5 parts, 4 parts of potassium hydroxide, 9 parts of disodium ethylene diamine tetraacetate, 7 parts of high energy oligosaccharides.
Based on the process of preparing of foregoing edible and medical fungi activity improver of the invention, according to the specific of the present invention
Embodiment, another object of the present invention are to provide the edible and medical fungi obtained as prepared by foregoing process of preparing of the invention
Use activity improver.Likewise, the related case study on implementation of the embodiment based on the present invention, still a further object of the present invention are
The edible and medical fungi activity improver is provided in preparing volume increase edible and medical fungi and/or improving the fertilizer of edible and medical fungi quality
Purposes.Wherein, the edible and medical fungi is preferably but not limited to white fungus.
Humic acid, a kind of polymer substance from peat, lignite or weathered coal in advance, there is substantial amounts of active group.
These active groups can effectively improve the activity of white fungus mycelia and the extracellular biology enzyme of incense ashes mycelia, the degraded of enhancing mycelium, inhale
Receipts, conveying and storage nutriment ability, later stage mycelia rejuvenation and regeneration, raising metabolism and production mushroom potentiality can be promoted.Remove
Outside this, in white fungus generative growth phase, adsorbing the mineral matter in humic acid can discharge, before effective supplementing culture medium
The mineral matter nutritional of phase consumption, enough mineral matters are provided for the growth of fructification.
Cornstarch extract is the deep processed product that high energy oligosaccharides is corn starch sugar, and processing technology is in high temperature height
Pressure is formed by decolouring, ion exchange, concentration.Outward appearance is white powder, pure natural, without any additive, without appointing
What chemical synthesis hormone, it is a kind of high-energy, the natural activity factor efficiently, green, safe, is provided for white fungus growth good
Good active material.Particularly containing isomalto-oligosaccharide, manna oligosacchride, glucan, biotin H isoreactivity materials, enzyme is participated in
Composition and bacterial metabolism.Growth white fungus mycelia and the activity of the various enzymes of incense ashes mycelia can be stimulated and adjusted, accelerates matrix organic nitrogen
Source is degraded with carbon source, is changed into the nutriments such as small-molecular peptides, amino acid, water-soluble sugar, is directly absorbed for white fungus mycelia,
And suppress the growth of harmful bacteria in compost, improve the immunity of mycelium and fructification, so as to improve edible mushroom quality,
Improve yield.
Amino acid can not only be used for nitrogen source but also as carbon source, can directly be absorbed by white fungus mycelia, be grown in white fungus
Journey is a kind of good energy supplement.White fungus mycelia and incense ashes mycelia nitrophilous, urea are a kind of good in white fungus growth course
Nitrogen source, can directly it be absorbed by mycelia, nitrogen can quickly be supplemented by adding appropriate urea.
EDTA-2Na (disodium ethylene diamine tetraacetate) is a kind of important complexing agent, for complexation of metal ions, it with magnesium,
Iron, zinc, manganese, copper isochela synthesis Chelating state element.Middle trace element is composition or the activation of a variety of enzymes in white fungus growth course
Agent, participate in carbon assimalation, carbohydrate transhipment, nitrogen metabolism and oxidation-reduction process etc.；Mycelial growth and Er Ji can be promoted
Formed, development, strengthen resistance.Because the metallic elements such as K, Mg, Fe, Cu, Zn, Mn are easily fixed, absorptivity is very low.With this
Technique is processed into chelated trace fertilizer, directly can directly be absorbed by white fungus mycelia and incense ashes mycelia, so not only directly supplement
Trace element, and utilization rate is substantially increased, white fungus yield can be increased with improving quality.
Based on above technical scheme content, the edible and medical fungi activity that the process of preparing based on the present invention is obtained
Improver, there is excellent technique effect in terms of edible and medical fungi growth is promoted, be embodied in lifting edible and medical fungi quality and
Two aspects of yield.Distinctive organic matter in the edible and medical fungi activity improver that the process of preparing of the present invention is obtained
Rich in active material, degraded, absorption, the conveying of (especially white fungus) mycelia of edible and medical fungi in matrix and incense ashes mycelia can be strengthened
And the ability of storage nutriment, promote later stage mycelia rejuvenation and regeneration.In further experimental comparison, preparation of the invention
The effect of increasing production for the edible and medical fungi activity improver that process is obtained is up to 10%~20%.Meanwhile edible and medical fungi
After (especially white fungus) matrix applies this product, the polyoses content of its fructification can improve 15% -30%, effectively improve mushroom
Quality.At the same time, preparation method provided by the present invention also has operation succinctly, easy to use, is easy to carry out industrialization
The technological merit such as popularization.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will to embodiment or
The required accompanying drawing used is briefly described in description of the prior art, it should be apparent that, drawings in the following description are only
Some embodiments of the present invention, for those of ordinary skill in the art, on the premise of not paying creative work, also
Other accompanying drawings can be obtained according to these accompanying drawings.
Fig. 1 is the process of preparing flow chart of the edible and medical fungi activity improver of the present invention.
Fig. 2 is the C batch experimental data situations in the embodiment 7 of the edible and medical fungi activity improver of the present invention.
Fig. 3 is A batch control group mycelial growth situation maps.
Fig. 4 is A batch experimental group mycelial growth situation maps.
Fig. 5 is B batches control group and experimental group mycelial growth situation map.
Fig. 6 is C batch control group mycelial growth situation maps.
Fig. 7 is C batch experimental group mycelial growth situation maps.
After Fig. 8 is inoculation 37 days, C batches experimental group and compares figure growing state figure.
Fig. 9 is that white fungus bacterium bag latter stage of ripening nutritional ingredient detects data drawing list.
Figure 10 is A batch experimental group mycelial growth situation maps.
Figure 11 is A batch control group mycelial growth situation maps.
Figure 12 is B batches experimental group and control group mycelial growth situation map.
Figure 13 is C batch experimental group mycelial growth situation maps.
Figure 14 is C batch control group mycelial growth situation maps.
Figure 15 is the average every weight of each group after drying with being incremented by amplitude chart.
Figure 16 is weights figure after A batch experimental group tremella fuciformis dryings.
Figure 17 is weights figure after A batch control group tremella fuciformis dryings.
Figure 18 is weights figure after B batch experimental group tremella fuciformis dryings.
Figure 19 is weights figure after B batch control group tremella fuciformis dryings.
Figure 20 is weights figure after C batch experimental group tremella fuciformis dryings.
Figure 21 is weights figure after C batch control group tremella fuciformis dryings.
Figure 22 is that Tremella fructification polysaccharide component detects data drawing list.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical scheme will be carried out below
Detailed description.Obviously, described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art institute resulting on the premise of creative work is not made
There is other embodiment, belong to the scope that the present invention is protected.
Embodiment 1
The present embodiment provides a kind of process of preparing of edible and medical fungi activity improver, used raw material components
It is as follows：Humic acid potassium 8kg, urea 20kg, amino acid 8kg, magnesium sulfate 10kg, potassium dihydrogen phosphate 10kg, zinc sulfate 2kg, boric acid
2kg, ferrous sulfate 2kg, copper sulphate 2kg, manganese chloride 2kg, potassium hydroxide 3kg, disodium ethylene diamine tetraacetate 8kg, high energy oligosaccharides
5kg。
The edible and medical fungi of the present embodiment is comprised the following steps that with the process of preparing of activity improver：
1st, humic acid potassium, urea, amino acid, potassium dihydrogen phosphate and boric acid are weighed, it is standby to obtain the first batch mixing after well mixed
With.
2nd, magnesium sulfate, zinc sulfate, ferrous sulfate, copper sulphate, manganese chloride and disodium ethylene diamine tetraacetate are weighed, is added anti-
The well mixed water for obtaining the second batch mixing, 0.5 times of second batch mixing gross weight being added into reactor of kettle is answered, is obtained after stirring reaction
To intermediate reaction liquid.
3rd, first batch mixing is added in the intermediate reaction liquid of reactor, is then kept stirring for continuing to reaction
The water of 0.5 times of first batch mixing gross weight is added in kettle, potassium hydroxide regulation pH to 7 is then added, finally adds high energy widow
Sugar, dry after stirring reaction, crush the edible and medical fungi activity improver for producing the present embodiment.
Embodiment 2：
The present embodiment provides a kind of process of preparing of edible and medical fungi activity improver, used raw material components
It is as follows：Humic acid potassium 8kg, urea 20kg, amino acid 8kg, magnesium sulfate 10kg, potassium dihydrogen phosphate 10kg, zinc sulfate 2kg, boric acid
2kg, ferrous sulfate 2kg, copper sulphate 2kg, manganese chloride 2kg, potassium hydroxide 3kg, disodium ethylene diamine tetraacetate 8kg, high energy oligosaccharides
5kg。
The edible and medical fungi of the present embodiment is comprised the following steps that with the process of preparing of activity improver：
1st, humic acid potassium, urea, amino acid, potassium dihydrogen phosphate and boric acid are weighed, it is standby to obtain the first batch mixing after well mixed
With.
2nd, magnesium sulfate, zinc sulfate, ferrous sulfate, copper sulphate, manganese chloride and disodium ethylene diamine tetraacetate are weighed, is added anti-
The well mixed water for obtaining the second batch mixing, 0.5 times of second batch mixing gross weight being added into reactor of kettle is answered, is stirred at 60 DEG C
Intermediate reaction liquid is obtained after reaction 150min.
3rd, the reactor internal temperature of 60 DEG C of holding is continued, the centre that first batch mixing is added to reactor is anti-
Answer in liquid, be then kept stirring for continuing adding the water of 0.5 times of first batch mixing gross weight into reactor, then add hydroxide
Potassium adjusts pH to 6.5, finally adds high energy oligosaccharides, is dried after stirring reaction, crushes the edible and medical fungi use for producing the present embodiment
Activity improver.
Embodiment 3：
The present embodiment provides a kind of process of preparing of edible and medical fungi activity improver, used raw material components
It is as follows：Humic acid potassium 8kg, urea 30kg, amino acid 8kg, magnesium sulfate 15kg, potassium dihydrogen phosphate 10kg, zinc sulfate 3kg, boric acid
2kg, ferrous sulfate 3kg, copper sulphate 2kg, manganese chloride 3kg, potassium hydroxide 3kg, disodium ethylene diamine tetraacetate 10kg, high energy are few
Sugared 5kg.
The edible and medical fungi of the present embodiment is comprised the following steps that with the process of preparing of activity improver：
1st, humic acid potassium, urea, amino acid, potassium dihydrogen phosphate and boric acid are weighed, it is standby to obtain the first batch mixing after well mixed
With.
2nd, magnesium sulfate, zinc sulfate, ferrous sulfate, copper sulphate, manganese chloride and disodium ethylene diamine tetraacetate are weighed, is added anti-
The well mixed water for obtaining the second batch mixing, 0.5 times of second batch mixing gross weight being added into reactor of kettle is answered, is stirred at 80 DEG C
Intermediate reaction liquid is obtained after reaction 120min.
3rd, the reactor internal temperature of 80 DEG C of holding is continued, the centre that first batch mixing is added to reactor is anti-
Answer in liquid, be then kept stirring for continuing adding the water of 0.5 times of first batch mixing gross weight into reactor, then add hydroxide
Potassium adjusts pH to 7, finally adds high energy oligosaccharides, is dried after stirring reaction, crushes the edible and medical fungi work for producing the present embodiment
Property improver.
High energy oligosaccharides in the present embodiment is feed grade high energy oligosaccharides, is cornstarch extract, voluntarily produces,
Specifically reparation technology is：The smart starch milk that protein content is less than 0.5% is chosen, through adjusting breast to make pH be 5.5-6.0, is added resistance to
High temperature enzyme, through high-temperature injection twice, wherein after spraying for the first time, maintained 90~120 minutes at 95 DEG C, spray temperature for the second time
Spend for 120~135 DEG C, 3~5 minutes time.After cooling, invertase is added, reaches defined purity through certain time conversion,
Filtered again, concentration, decrease temperature crystalline, it is high energy oligosaccharides to crush, dry.
Embodiment 4：
The present embodiment provides a kind of process of preparing of edible and medical fungi activity improver, used raw material components
It is as follows：Humic acid potassium 13kg, urea 20kg, amino acid/11 0kg, magnesium sulfate 10kg, potassium dihydrogen phosphate 15kg, zinc sulfate 2kg, boron
Sour 3kg, ferrous sulfate 2kg, copper sulphate 3kg, manganese chloride 2kg, potassium hydroxide 5kg, disodium ethylene diamine tetraacetate 8kg, high energy are few
Sugared 9kg.
The edible and medical fungi of the present embodiment is comprised the following steps that with the process of preparing of activity improver：
1st, humic acid potassium, urea, amino acid, potassium dihydrogen phosphate and boric acid are weighed, it is standby to obtain the first batch mixing after well mixed
With.
2nd, magnesium sulfate, zinc sulfate, ferrous sulfate, copper sulphate, manganese chloride and disodium ethylene diamine tetraacetate are weighed, is added anti-
The well mixed water for obtaining the second batch mixing, 0.5 times of second batch mixing gross weight being added into reactor of kettle is answered, is stirred at 70 DEG C
Intermediate reaction liquid is obtained after reaction 135min.
3rd, the reactor internal temperature of 70 DEG C of holding is continued, the centre that first batch mixing is added to reactor is anti-
Answer in liquid, be then kept stirring for continuing adding the water of 0.5 times of first batch mixing gross weight into reactor, then add hydroxide
Potassium adjusts pH to 7, finally adds high energy oligosaccharides, is dried after stirring reaction, crushes the edible and medical fungi work for producing the present embodiment
Property improver.
The edible and medical fungi of acquisition is respectively with the weight ratio of each nutritional ingredient in activity improver prepared by the present embodiment：
Humic acid >=4%, amino acid >=8%, N+P+K >=20%, wherein N >=10%, Mg+Zn+Fe+Cu+Mn+B >=4%, it is active because
Sub (high energy oligosaccharides) >=5%, pulvis is obtained after crushing, the mesh number of pulvis is more than 100 mesh and uniform, and moisture is less than
0.5%, pH value is between 6.5~7.5.
Embodiment 5：
The present embodiment provides a kind of process of preparing of edible and medical fungi activity improver, used raw material components
It is as follows：Humic acid potassium 11kg, urea 25kg, amino acid 9kg, magnesium sulfate 12kg, potassium dihydrogen phosphate 13kg, zinc sulfate 2.5kg,
Boric acid 2.5kg, ferrous sulfate 2.5kg, copper sulphate 2.5kg, manganese chloride 2.5kg, potassium hydroxide 4kg, disodium ethylene diamine tetraacetate
9kg, high energy oligosaccharides 7kg.Wherein, humic acid potassium is weathered coal extract.
The edible and medical fungi of the present embodiment is comprised the following steps that with the process of preparing of activity improver：
1st, humic acid potassium, urea, amino acid, potassium dihydrogen phosphate and boric acid are weighed, it is standby to obtain the first batch mixing after well mixed
With.
2nd, magnesium sulfate, zinc sulfate, ferrous sulfate, copper sulphate, manganese chloride and disodium ethylene diamine tetraacetate are weighed, is added anti-
The well mixed water for obtaining the second batch mixing, 0.5 times of second batch mixing gross weight being added into reactor of kettle is answered, is stirred at 70 DEG C
Intermediate reaction liquid is obtained after reaction 135min.
3rd, the reactor internal temperature of 70 DEG C of holding is continued, the centre that first batch mixing is added to reactor is anti-
Answer in liquid, be then kept stirring for continuing adding the water of 0.5 times of first batch mixing gross weight into reactor, then add hydroxide
Potassium adjusts pH to 7, finally adds high energy oligosaccharides, is dried after stirring reaction, crushes the edible and medical fungi work for producing the present embodiment
Property improver.
Embodiment 6：
The present embodiment provides a kind of process of preparing of edible and medical fungi activity improver, used raw material components
It is as follows：Humic acid potassium 11kg, urea 25kg, amino acid 9kg, potassium dihydrogen phosphate 13kg, boric acid 2.5kg, potassium hydroxide 4kg, second
Edetate disodium 9kg, high energy oligosaccharides 7kg.There are magnesium sulfate, zinc sulfate, sulfuric acid sub- in the preparation component of the present embodiment
Iron, copper sulphate and manganese chloride, magnesium sulfate, zinc sulfate, ferrous sulfate, copper sulphate, manganese chloride and disodium ethylene diamine tetraacetate rub
You are followed successively by 0.2 by ratio:0.2:0.2:0.2:0.2:1.
The edible and medical fungi of the present embodiment is comprised the following steps that with the process of preparing of activity improver：
1st, humic acid potassium, urea, amino acid, potassium dihydrogen phosphate and boric acid are weighed, it is standby to obtain the first batch mixing after well mixed
With.
2nd, magnesium sulfate, zinc sulfate, ferrous sulfate, copper sulphate, manganese chloride and disodium ethylene diamine tetraacetate are weighed, is added anti-
The well mixed water for obtaining the second batch mixing, 0.5 times of second batch mixing gross weight being added into reactor of kettle is answered, is stirred at 70 DEG C
Intermediate reaction liquid is obtained after reaction 135min.
3rd, the reactor internal temperature of 70 DEG C of holding is continued, the centre that first batch mixing is added to reactor is anti-
Answer in liquid, be then kept stirring for continuing adding the water of 0.5 times of first batch mixing gross weight into reactor, then add hydroxide
Potassium adjusts pH to 7, finally adds high energy oligosaccharides, is dried after stirring reaction, crushes the edible and medical fungi work for producing the present embodiment
Property improver.
Embodiment 7：
In order to further verify the technology of edible and medical fungi activity improver that foregoing embodiments of the invention prepare
Effect, the present invention has also carried out series of contrast, specific as follows：
Test material and method
1st, test material
(1) bacterial strain：White fungus.(2) culture medium：Cotton seed hulls, wheat bran, gypsum.(3) foregoing embodiments of the invention are prepared and obtained
The edible and medical fungi activity improver obtained.
2nd, test method
2.1 experimental design：This experiment sets tri- batches of A, B, C, and test group and control group, three batch examinations are set per batch
The all identical i.e. cotton seed hulls 87% of culture medium prescription, wheat bran 12%, the gypsum 1% of group and control group are tested, per described in batch experimental group
The addition of edible and medical fungi activity improver is different.From 13cm × 58cm low-pressure polyethylene polybag, every bag of average dress
About 1.4 jin of siccative.Sterilizing is using local conventional atmospheric steam sterilizing (100 DEG C, 20 hours).Tremella cultivation house uses local agriculture
Family ear canopy.Culture is with management according to working as ground mode.Experimental group bacteria stick intersects with control group bacteria stick, different in different ear canopies
Sortie, the adjacent experimental group of diverse location respectively choose 100 bacteria sticks with control group as experiment gathered data.Observation note per week
Mycelium germination of record and Tremella fructification growth change situation,
2.2 test periods and place
2.2.1A batch：In October, 2016 in December, 2016, city east street MaDang Cun, 370 meters of height above sea level.
2.2.2B batch：In December, 2016 in March, 2017, Gutian County bridge town Qian Cuo villages, 800 meters of height above sea level.
2.2.3C batch：2 months in April, 2017 in 2017, Gutian County bridge town Qian Cuo villages, 800 meters of height above sea level.
2.3 test procedure
2.3.1A batch inoculation on October 15th, 2016, unified harvesting on November 28th, 2016.November 29 dried.
(1) control group
According to cotton seed hulls 87%, wheat bran 12%, gypsum 1% formula, cotton seed hulls is prewetted left to water content 55%~60%
The right side, wheat bran is sprinkled on cotton seed hulls, is finally sprinkled into gypsum, packed after uniform mixing, beat cave (every bag is made a call to three caves), seal cave
Mouthful, sterilizing, cooling, inoculation, culture and ear management be according to working as ground mode.
(2) test group
Formula：Cotton seed hulls 1150 jin (87%), wheat bran 160 jin (12%), gypsum 13 jin (1%), edible and medical fungi activity
Improver 5.3 jin (0.4%).Cotton seed hulls is prewetted to water content 55% -60% or so, wheat bran is sprinkled on cotton seed hulls, then spread
Enter gypsum, be finally sprinkled into after edible and medical fungi activity improver uniform mixing to pack and mark, beat cave, seal pocket after be mixed into
Control group, together sterilizing, cooling, inoculation, culture and ear management.945 bags altogether.
2.3.2B batch inoculation on December 21st, 2016, unified harvesting on 2 18th, 2017, is dried on the 19th.
(1) control group
According to cotton seed hulls 87%, wheat bran 12%, gypsum 1% formula, cotton seed hulls is prewetted left to water content 55% -60%
The right side, wheat bran is sprinkled on cotton seed hulls, is finally sprinkled into gypsum, packed after uniform mixing, beat cave (every bag is made a call to three caves), seal cave
Mouthful, sterilizing, cooling, inoculation, culture and ear management be according to working as ground mode.
(2) test group
Formula：Cotton seed hulls 720 jin (87%), wheat bran 100 jin (12%), gypsum 10 jin (1%), edible and medical fungi activity
Improver 1.7 jin (0.2%).Cotton seed hulls is prewetted to water content 55% -60% or so, wheat bran is sprinkled on cotton seed hulls, then spread
Enter gypsum, be finally sprinkled into after edible and medical fungi activity improver uniform mixing to pack and mark, beat cave, seal pocket after be mixed into
Control group, together sterilizing, cooling, inoculation, culture and ear management.580 bags altogether.
2.3.3C batch：It is inoculated with 2 28th, 2017, on April 21st, 2017 plucks because high temperature shifts to an earlier date a week, 22
Dry
(1) control group
According to cotton seed hulls 87%, wheat bran 12%, gypsum 1% formula, cotton seed hulls is prewetted left to water content 55% -60%
The right side, wheat bran is sprinkled on cotton seed hulls, is finally sprinkled into gypsum, packed after uniform mixing, beat cave (every bag is made a call to three caves), seal cave
Mouthful, sterilizing, cooling, inoculation, culture and ear management be according to working as ground mode.
(2) test group：
Formula：Cotton seed hulls 580 jin (87%), wheat bran 80 jin (12%), gypsum 7 jin of (1%), edible and medical fungis are changed with activity
Kind agent 2 jin (0.3%).Cotton seed hulls is prewetted to water content 55% -60% or so, wheat bran is sprinkled on cotton seed hulls, then be sprinkled into stone
Cream, be finally sprinkled into after edible and medical fungi activity improver uniform mixing to pack and mark, beat cave, seal pocket after be mixed into control
Group, together sterilizing, cooling, inoculation, culture and ear management.468 bags altogether.
3rd, result of the test
Three batch experimentations are because cotton seed hulls quality is different, and the yield height of each batch is also different, simultaneously because height above sea level
Height is all different from temperature, and particularly B batches, C batches are in high altitude localities, and temperature is relatively low, therefore growth cycle is longer, to reality
Testing result has certain influence.
3.1 mycelium germination growing states
Three batch experimental groups are more with the obvious advantage than control group, show as that mycelium germination is sturdy, dense, pure white, vitality is prosperous
Contain, it is fast to walk bacterium speed, and accompanying drawing 2 is C batch white fungus mycelium germinations speed mounting flower record data table.
3.1.1A batch mycelial growth contrasts
Referring to accompanying drawing 3 and accompanying drawing 4, Fig. 3 is A batch control group mycelial growth situations, and Fig. 4 gives birth to for A batch experimental groups mycelia
Long situation.
3.1.2B batch mycelial growth contrasts
Referring to accompanying drawing 5, Fig. 5 is B batches control group and experimental group mycelial growth situation, left as shown in mark in accompanying drawing 5
Side is control group, and right side is experimental group.
3.1.3C batch mycelial growth contrasts
Referring to accompanying drawing 6 and accompanying drawing 7, Fig. 6 is C batch control group mycelial growth situations, and Fig. 7 gives birth to for C batch experimental groups mycelia
Long situation.
After 3.2 inoculations 37 days, bacteria stick nutritional ingredient sugar increases considerably, and C batch growing states are such as attached as shown in Fig. 8
In Fig. 8 shown in mark, left side is experimental group, and right side is control group.As shown in figure 9, it is white fungus bacterium bag latter stage of ripening nutrition in Fig. 9
Composition detection data drawing list.
3.3 sporophore growth phase piece types are big, rounding, well-balanced, and auricle thickness is flexible, whiteness is good.
3.3.1A batch sporophore growth contrasts
Referring to accompanying drawing 10 and accompanying drawing 11, Figure 10 is A batch experimental group mycelial growth situations, and Figure 11 is A batch control group bacterium
Silk growing state.
3.3.2B batch sporophore growth contrasts
Referring to accompanying drawing 12, Figure 12 is B batches experimental group and control group mycelial growth situation, left as shown in mark in Figure 12
Side is experimental group, and right side is control group.
3.3.3C batch sporophore growth contrasts
Referring to accompanying drawing 13 and accompanying drawing 14, Figure 13 is C batch experimental group mycelial growth situations, and Figure 14 is C batch control group bacterium
Silk growing state.
Each batch is respectively selected in different ear canopies, different sorties, the adjacent experimental group of diverse location with control group during 3.4 harvesting
30 bacteria sticks are taken to remove impurity, the rinsings such as the ear base of a fruit after harvesting as experimental data, dry, weigh, averagely increase production 8.9%.Its
Middle A batches experimental group volume increase 6.2%, B batches experimental group volume increase 10.3%, C batches experimental group volume increase 10.2%.Three batches
Data see the table below 1：
The average every weight of each group and incremental amplitude after table 1 is dried
The average every weight of each group is with being incremented by amplitude referring to accompanying drawing 15 after drying.
3.4.1A white fungus weight after batch drying
It is weights after A batch experimental group tremella fuciformis dryings referring to accompanying drawing 16 and 17, Figure 16, Figure 17 is A batch control groups
Weights after tremella fuciformis drying.
3.4.2B white fungus weight after batch drying
It is weights after B batch experimental group tremella fuciformis dryings referring to accompanying drawing 18 and 19, Figure 18, Figure 19 is B batch control groups
Weights after tremella fuciformis drying.
3.4.3C white fungus weight after batch drying
It is weights after C batch experimental group tremella fuciformis dryings referring to accompanying drawing 20 and 21, Figure 20, Figure 21 is C batch control groups
Weights after tremella fuciformis drying.
3.5 Tremella fructification qualities increase substantially, and tremella polysaccharides can improve more than 15%.Specific testing result is shown in attached
Figure 22.
4 conclusions and analysis
The growth cycle of 4.1 white fungus about 40 days or so.This experiment is selection Gutian cultivation mode, and ear canopy is to utilize locality
Special ear canopy, heat-insulating property are relatively poor.B, C batch experiments site elevation is high, and temperature is low, ear canopy indoor air temperature during bacteria
Average just 16-18C °, mycelium germination speed is slow, therefore the bacteria phase has been grown nearly 20 days compared with A batches, and whole growth cycle is than A batch
Also extend more than ten 20 days, there is certain influence on experimental result.
The fresh ear experimental group of 4.2C batches averagely increases production 10.96% than control group, and conversion ratio improves 6%.Because of C batch screech owls
Later stage runs into high temperature, and ear canopy indoor temperature causes rotten ear more than 30C °, it has to proposes previous week harvesting, bacterium bag nutrient is also
Surplus nearly 30% is not absorbed, and has a strong impact on yield.So the yield of C batches can not reflect the batch true production, contrast effect
There is certain influence.After 30 fresh ears of C batches remove the impurity such as the ear base of a fruit, control group 11.4kg is average 126.66g/, 380g/
Cylinder, conversion ratio 54.2%；Experimental group 12.65kg, average 140.55g/, 421.66g/ cylinders, conversion ratio 60.2%.
Tri- batch experiments of 5.3A, B, C are played to white fungus increasing yield and improving quality effect, illustrate to add culture medium siccative
0.2% -0.4% edible and medical fungi activity improver can play increasing yield and improving quality effect, comprehensive nutrient growth period and reproduction
Growth period effect, wherein being behaved oneself best especially with C batches, it is proposed that the edible and medical fungi activity improver optimum addition in spice
For 0.3% (1000 kilograms of siccatives add 3 kilograms of edible and medical fungi activity improvers) of culture medium siccative.
The mechanism of 5.4 edible and medical fungis activity improver increasing yield and improving quality.According to produce agaric bacteria stick at initial stage mycelium nutritional ingredient
Testing result, experimental group total reducing sugar are higher than control group body by 14.38%；Tremella fructification polyoses content test result, experimental group
Than control group increase by 18.8%.It is wooden that this explanation edible and medical fungi activity improver can promote white fungus mycelia to be degraded with incense ashes mycelia
Quality, cellulose and hemicellulose, increase sucrose accumulation.Sugar is that the part of fructification is energy again, is volume increase
It is crucial.It is living that edible and medical fungi activity improver contains humic acid macromolecule, oligosaccharide, manna oligosacchride, glucan, biotin H etc.
Property material and mineral matter, these active materials participate in the composition and bacterial metabolism of enzyme, can stimulate and adjust growth white fungus mycelia with
The activity of the various enzymes of incense ashes mycelia, accelerate matrix organic nitrogen source and carbon source and degrade, for white fungus mycelia directly absorb carbon,
The nutriments such as nitrogen, inorganic salts, and suppress the growth of harmful bacteria in compost, the immunity of mycelium and fructification is improved,
So as to improve edible mushroom quality, yield is improved.
Accordingly, foregoing activity improver of the invention is also provided in the lump and made referring to foregoing individual embodiments, the present invention
Purposes in standby volume increase edible and medical fungi and/or the fertilizer of improvement edible and medical fungi quality, the edible and medical fungi especially include white fungus,
The edible and medical fungi activity improver of the present invention can be bonded white fungus growth characteristics, be obviously improved white fungus yield and quality.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, and appoints
What those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, all should
It is included within the scope of the present invention.Therefore, protection scope of the present invention should using the scope of the claims as
It is accurate.
Claims (10)
1. a kind of process of preparing of edible and medical fungi activity improver, it is characterised in that comprise the following steps：
The first step, humic acid potassium, urea, amino acid, potassium dihydrogen phosphate and boric acid are weighed, it is standby to obtain the first batch mixing after well mixed
With；
Second step, magnesium sulfate, zinc sulfate, ferrous sulfate, copper sulphate, manganese chloride and disodium ethylene diamine tetraacetate are weighed, added anti-
Answer kettle is well mixed to obtain the second batch mixing, water is added into reactor, intermediate reaction liquid is obtained after stirring reaction；
3rd step, first batch mixing is added in the intermediate reaction liquid of reactor, be then kept stirring for continuing to reaction
Water is added in kettle, potassium hydroxide regulation pH to 6.5~7.5 is then added, finally adds high energy oligosaccharides, dried after stirring reaction
Do, crush and produce.
2. the process of preparing of a kind of edible and medical fungi activity improver according to claim 1, it is characterised in that each
The parts by weight usage ratio of the raw material of use is respectively：8~13 parts of humic acid potassium, 20~30 parts of urea, 8~10 parts of amino acid,
10~15 parts of magnesium sulfate, 10~15 parts of potassium dihydrogen phosphate, 2~3 parts of zinc sulfate, 2~3 parts of boric acid, 2~3 parts of ferrous sulfate, sulfuric acid
2~3 parts of copper, 2~3 parts of manganese chloride, 3~5 parts of potassium hydroxide, 8~10 parts of disodium ethylene diamine tetraacetate, 5~9 parts of high energy oligosaccharides.
A kind of 3. process of preparing of edible and medical fungi activity improver according to claim 1, it is characterised in that the
Magnesium sulfate in two steps, zinc sulfate, ferrous sulfate, the amount of total material and the thing of disodium ethylene diamine tetraacetate of copper sulphate and manganese chloride
The ratio of the amount of matter is 1:1.
A kind of 4. process of preparing of edible and medical fungi activity improver according to claim 1, it is characterised in that the
In two steps, the reaction time of stirring reaction is 120~150min, and the reaction temperature of stirring reaction is 60~80 DEG C.
A kind of 5. process of preparing of edible and medical fungi activity improver according to claim 1, it is characterised in that the
In three steps, reactor temperature is kept to carry out the addition of the first batch mixing under the conditions of 60~80 DEG C.
A kind of 6. process of preparing of edible and medical fungi activity improver according to claim 1, it is characterised in that institute
It is feed grade high energy oligosaccharides to state high energy oligosaccharides, is cornstarch extract.
A kind of 7. process of preparing of edible and medical fungi activity improver according to claim 6, it is characterised in that institute
The reparation technology for stating high energy oligosaccharides is：Choose the smart starch milk that protein content is less than 0.5%, through adjust breast make pH for 5.5-
6.0, high temperature resistant enzyme is added, through high-temperature injection twice, wherein after spraying for the first time, maintains 90~120 minutes at 95 DEG C, second
The secondary temperature that sprays is 120~135 DEG C, 3~5 minutes time.After cooling, conversion enzymatic conversion, then filtered, concentration, cooling are added
Crystallization, it is high energy oligosaccharides to crush, dry.
A kind of 8. process of preparing of edible and medical fungi activity improver according to claim 1, it is characterised in that the
Obtained edible and medical fungi is respectively with the weight accounting of each nutritional ingredient in activity improver after three steps crush：Humic acid >=4%,
Amino acid >=8%, N+P+K >=20%, N >=10%, Mg+Zn+Fe+Cu+Mn+B >=4%, active factors >=5%.
A kind of 9. process of preparing of edible and medical fungi activity improver according to claim 8, it is characterised in that the
The pulvis mesh number for the edible and medical fungi activity improver that three steps obtain after crushing is more than 100 mesh and uniform, and moisture is less than
0.5%, pH value is between 6.5~7.5.
A kind of 10. process of preparing of edible and medical fungi activity improver according to claim 1, it is characterised in that
The weight of the water added in second step into reactor is 0.5 times of the second batch mixing gross weight；Add in 3rd step into reactor
The weight of the water entered is 0.5 times of the first batch mixing gross weight.
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