CN107353121A - A kind of liquid microbe fertilizer for preventing and treating cucumber fungal disease and preparation method thereof - Google Patents
A kind of liquid microbe fertilizer for preventing and treating cucumber fungal disease and preparation method thereof Download PDFInfo
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- CN107353121A CN107353121A CN201710722619.7A CN201710722619A CN107353121A CN 107353121 A CN107353121 A CN 107353121A CN 201710722619 A CN201710722619 A CN 201710722619A CN 107353121 A CN107353121 A CN 107353121A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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Abstract
The present invention relates to a kind of liquid microbe fertilizer and preparation method thereof for preventing and treating cucumber fungal disease, include the raw material of following weight parts：50~100 parts of streptomyces microflavus zymotic fluid, 60~80 parts of calcium carbonate, 10~40 parts of magnesium sulfate, 0.5~1 part of borax, 0.5~1 part of ferric sulfate, 0.1~0.5 part of manganese sulfate, 0.001~0.01 part of zinc sulfate, 0.001~0.007 part of copper sulphate, 0.001~0.005 part of ammonium molybdate；Its preparation method prepares seaweed extracted liquor including ultrasonic wave added enzymolysis, the seaweed extracted liquor of preparation is configured to fermentation medium again, streptomyces microflavus is cultivated under certain condition, and finally by zymotic fluid and middle trace element built, liquid microbe fertilizer of the invention is made.Of the invention gained liquid microbe fertilizer formula science, rationally, nutrition is comprehensive, abundant, the needs of Plant Growth of Cucumber can not only be met, Disease Resistance of Cucumber can also be effectively improved, prevent and treat fungal disease, and cucumber quality can be improved, cucumber yield and edible safety are improved, for promoting green agriculture development and pollution-free food production significant.
Description
Technical field
The invention belongs to microbial manure technical field, and in particular to a kind of liquid microbe for preventing and treating cucumber fungal disease
Fertilizer and preparation method thereof.
Background technology
Cucumber, alias king melon, cucumber, it is the annual climber herbaceous plant of Curcurbitaceae Cucumis, originating from Himalaya
The tropical rain forest area of mountain southern foot.Cucumber has the characteristics that yield is high, nutritious, profitable, be cultivate in the world it is most universal
A kind of melon vegetables, and one of staple vegetable crop that China's cultivated area is maximum, planting range is most wide.
Fungal disease, such as powdery mildew, downy mildew, it is the disease generally occurred in cucumber cultivation, the popular time can the underproduction
30~50%.Mainly include on preventing and treating the method for cucumber fungal disease both at home and abroad：From resistant variety, chemical pesticide control,
Physical control, biological control and bionomic control etc., wherein, chemical pesticide control is most common prevention and controls.However, agricultural chemicals
Unreasonable use does not reach expected prevention effect not only, and easily causes poisoning, pollutes environment, increases on agricultural product
Persticide residue；The blindness of agricultural chemicals, which is applied, can also kill natural enemy, strengthen the resistance to the action of a drug of germ or insect, further increase pest and disease damage
The difficulty of preventing and treating, increase production cost.Therefore, during cucumber production reasonable prevention and controls selection, to reduce pathogen production
The raw resistance to the action of a drug, reduce chemical agent amount of application, reduce environmental pollution, to improve cucumber edible safety significant.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to provide a kind of liquid microbe for preventing and treating cucumber fungal disease
Fertilizer, effectively solves that cucumber harmful substance or residues of pesticides, pathogen are developed immunity to drugs in the prior art and environmental pollution etc. is asked
Topic, and Disease Resistance of Cucumber can be effectively improved, fungal disease is prevented and treated, improves cucumber quality, improves cucumber yield and edible safety, it is right
In promoting, green agriculture development and pollution-free food production are significant.
The present invention is achieved through the following technical solutions：
A kind of liquid microbe fertilizer for preventing and treating cucumber fungal disease, include the raw material of following weight parts：Thin yellow strepto-
50~100 parts of fermented liquid, 60~80 parts of calcium carbonate, 10~40 parts of magnesium sulfate, 0.5~1 part of borax, 0.5~1 part of ferric sulfate,
0.1~0.5 part of manganese sulfate, 0.001~0.01 part of zinc sulfate, 0.001~0.007 part of copper sulphate, ammonium molybdate 0.001~0.005
Part.
Preferably, a kind of liquid microbe fertilizer for preventing and treating cucumber fungal disease, the raw material of following weight parts are included：Carefully
60~70 parts of yellow streptomycete zymotic fluid, 65~75 parts of calcium carbonate, 20~30 parts of magnesium sulfate, 0.6~0.8 part of borax, ferric sulfate 0.6
~0.8 part, 0.2~0.4 part of manganese sulfate, 0.003~0.007 part of zinc sulfate, 0.003~0.005 part of copper sulphate, ammonium molybdate
0.002~0.004 part.
Preferably, a kind of liquid microbe fertilizer for preventing and treating cucumber fungal disease, the raw material of following weight parts are included：Carefully
65 parts of yellow streptomycete zymotic fluid, 70 parts of calcium carbonate, 28 parts of magnesium sulfate, 0.7 part of borax, 0.7 part of ferric sulfate, 0.3 part of manganese sulfate, sulphur
Sour 0.005 part of zinc, 0.004 part of copper sulphate, 0.003 part of ammonium molybdate.
The present invention also provides a kind of preparation method for the liquid microbe fertilizer for preventing and treating cucumber fungal disease, including following step
Suddenly：
(1) preparation of streptomyces microflavus zymotic fluid：
A, the preparation of seaweed extracted liquor：200~400 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6
Amount add deionized water, heat enzyme deactivation, obtain suspension；The pH to 7.0 of suspension is adjusted, adds α~amylase, neutral protein
Enzyme, cellulase, non-starch polysaccharide enzyme, digest under ultrasonic assistant effect, during which at the uniform velocity stirred using mixer；Enzymolysis knot
Enzyme deactivation, centrifugation, take supernatant, obtain seaweed extracted liquor after beam；
B, the preparation of fluid nutrient medium：Glucose, yeast juice, di(2-ethylhexyl)phosphate are added into seaweed extracted liquor obtained by step (A)
Hydrogen potassium, sodium chloride, ferrous sulfate, addition be respectively seaweed extracted liquor quality 1~3%, 1~2%, 0.01~0.05%,
0.02~0.05%, 0.001~0.005%, pH is adjusted to 7.0~7.4；
C, the preparation of zymotic fluid：Into the fermentation tank equipped with the fluid nutrient medium prepared by step (B), after inoculation activation
Streptomyces microflavus, oscillation and fermentation cultivation, in obtained zymotic fluid the CFU of streptomyces microflavus for streptomyces microflavus >=
2.0×109cfu/mL；
(2) preparation of liquid microbe fertilizer：
Calcium carbonate, magnesium sulfate, borax, ferric sulfate, manganese sulfate, zinc sulfate, copper sulphate, ammonium molybdate and step are weighed by weight
Suddenly streptomyces microflavus zymotic fluid obtained by (1), be well mixed, both liquid microbe fertilizer of the present invention.
Preferably, α~amylase in step (1), neutral proteinase, cellulase, non-starch polysaccharide enzyme addition according to
It is secondary for 3~5%, 1~2%, 2~3%, the 1~2% of marine alga quality.
Preferably, enzymatic hydrolysis condition is in step (1)：50~70 DEG C, ultrasonic power 300W of hydrolysis temperature, enzymolysis time 30
~60min.
Preferably, streptomyces microflavus inoculation percentage by weight is 1%~5% in step (1).
Preferably, fermentation culture conditions are in step (1)：28~32 DEG C, 4~6d of incubation time of cultivation temperature, vibration training
It is 160~260rpm/min to support rotating speed.
Each composition mechanism of action of the present invention is as follows：
Marine alga：Marine alga is that most wide biology is distributed in ocean, and species is various, there is Chlorophyta, Phaeophyta, Cyanophyta, red algae
Door etc., abundant sargassum protein, fat, vitamin, mineral matter and the physiology with special efficacy are contained in these fronds
Active material (the dirty acid of such as algal polysaccharides, algae, enzyme, various plants hormone, polyphenol), plant growth can be induced and reduce non-life
The mechanism of thing stress, the defence capability and osmotic protection function of crop are excited, and it is nontoxic pollution-free, it is the head of pollution-free food
Select fertilizer.Secondly, alginic acid contained in marine alga, has the function that complexation of metal ions, and it is coordinated with middle trace element makes
With nutrient can be kept, fertilizer loss is reduced, improves utilization rate of fertilizer.
Streptomyces microflavus：Streptomyces microflavus is a kind of resourceful actinomyces, can produce netropsin, spiramvcin etc.
Antibacterial material, suppress Gram-positive and negative bacteria, saccharomycete, the growth and breeding of filamentous fungi, promote plant growth, prevention
Disease occurs.Research shows that streptomyces microflavus shows to pathogen of crop such as downy mildew of alfalfa, powdery mildew, brown spot, root rot
Good prevention effect is shown, the upper diseases prevention that is often used in agriculture is kept a full stand of seedings；Streptomyces microflavus also acts as bacterial manure, converts nitrogen phosphorus in soil
Element, increase soil fertility, and play the role of to stimulate plant growth.
Calcium：Calcium is the constituent of cell membrane mesoglea, enables cell to be tied to form tissue with cell, and make plant
Organ or individual there is certain mechanical strength；Secondly, calcium has a certain impact to nitrogen metabolism in crop body, and can neutralize work
The organic acid formed in thing metabolic process, to stablize intracellular environment play the role of it is important；Calcium can also lower plasm colloid
Decentralization, water-filling degree, viscosity, elasticity and permeability of cell etc. is maintained normal physiological status, be advantageous to crop just
Often metabolism.Tomato genus likes the vegetable crop of calcium, reacts sensitive to calcium, and during tomato calcium deficiency, plant is thin and weak, and root system is undeveloped, and meeting
Induce physiological disturbance, such as blossom-end rot.
Magnesium：Magnesium is the constituent of plant chlorophyll and polysome, and the activator of a variety of enzymes, can promote to make object
Interior vitamin A, ascorbic formation.Therefore, the synthesis of the photosynthesis and protein of crops can be promoted using fertiliser containing magnesium, carried
High crop yield, improve quality of agricultural product.According to another report, magnesium can also promote absorption of the plant to silicon, be advantageous to prevent germ
Intrusion.
Boron：Boron is the constituent of a variety of enzymes of plant, participates in the generation of the synthesis of plant vivo protein, nitrogen and carbohydrate
Thank.Boron can promote the normal operation of carbohydrate and the normal development of reproductive organs, improve the supply of oxygen, the development to root system
And the formation of fruit, seed has a major impact.Secondly, boron can also adjust absorption and oxidation-reduction process of the plant to moisture,
Also the formation of Legume nodule can be promoted.
Iron：Iron participates in the synthesis of chlorophyll, and it is the composition of some enzymes of composition and protein, participates in the oxygen in plant
The manufacture of change-reduction process and carbohydrate.
Manganese：Manganese participates in the assimilation of carbon dioxide in the metabolism of protein and inorganic acid, photosynthesis, point of carbohydrate
Formation of solution and carrotene, riboflavin and ascorbic acid etc..The accumulation transhipment of formation and carbohydrate of the manganese to chlorophyll, seed
Sprout, growth of seedling and plant is solid etc. good facilitation.Manganese is the activator of a variety of enzymes, to plant respiration, egg
White matter is decomposed, nitrate-nitrogen reduction is respectively provided with remarkable effect.
Zinc：Zinc be growth and development of plants necessary to trace element, be many enzymes in plant constituent and activator,
The formation of auximone is directly participated in, Substance P hydrolysis, oxidation-reduction process in plant are played an important role, can be also catalyzed
Protein synthesizes, and promotes seed maturity etc..
Copper：Copper is the composition of a variety of oxidizing ferment of composition in plant, and the catalyst of respiration, and it participates in chlorophyll
Synthesis and the metabolism of carbohydrate and protein.Copper also have improve chlorophyll stability ability, avoid chlorophyll too early by
Destroy, be advantageous to blade and preferably carry out photosynthesis.
Molybdenum：Molybdenum is present among biocatalyst, participates in symbiosis biological nitrogen fixation and the synthesis of protein, and promote photosynthetic
The intensity of effect.Secondly, plant relies on molybdenum and carries out nitrate reductase, eliminates activated aluminum in acid soil and accumulates and produce in plant
Raw toxic action.
Beneficial effects of the present invention：
1st, the present invention prepares seaweed extracted liquor using the method for ultrasonic wave added enzymolysis, avoids strong in marine alga process
The destruction of alkali, high temperature, high pressure, the nutriment enriched in marine alga is fully retained；
2nd, the present invention digests ultrasonic wave added the fermented and cultured that the seaweed extracted liquor prepared is used for streptomyces microflavus, gained hair
Zymotic fluid has marine alga activity and the live body regulatory function of streptomyces microflavus concurrently, and it is active organic with bacteria agent to realize seaweed bio
It is unified；
3rd, the present invention is by streptomyces microflavus zymotic fluid and middle trace element built, gained liquid fertilizer scientific formulation, reasonable,
Nutrition is comprehensive, abundant, can not only meet the needs of Plant Growth of Cucumber, can also effectively improve Disease Resistance of Cucumber, prevents and treats nosomycosis
Evil, and cucumber quality can be improved, cucumber yield and edible safety are improved, for promoting green agriculture development and pollution-free food production
It is significant.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Embodiment 1
A kind of liquid microbe fertilizer for preventing and treating cucumber fungal disease, include the raw material of following weight parts：Thin yellow strepto-
50 parts of fermented liquid, 60 parts of calcium carbonate, 30 parts of magnesium sulfate, 0.8 part of borax, 0.5 part of ferric sulfate, 0.1 part of manganese sulfate, zinc sulfate
0.003 part, 0.001 part of copper sulphate, 0.004 part of ammonium molybdate.
A kind of preparation method of above-mentioned liquid microbe fertilizer for preventing and treating cucumber fungal disease, comprises the following steps：
(1) preparation of streptomyces microflavus zymotic fluid：
A, the preparation of seaweed extracted liquor：400 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6 amount adds
Enter deionized water, heat enzyme deactivation, obtain suspension；The pH to 7.0 of suspension is adjusted, adds α~amylase, neutral proteinase, fibre
Plain enzyme, non-starch polysaccharide enzyme are tieed up, its addition is followed successively by 3.5%, 1.3%, 2.3%, the 1.3% of marine alga quality, in ultrasonic wave
Digest, 55 DEG C of hydrolysis temperature, ultrasonic power 300W, enzymolysis time 50min, during which at the uniform velocity stirred using mixer under booster action
Mix；Enzymolysis terminates rear enzyme deactivation, centrifugation, takes supernatant, obtains seaweed extracted liquor；
B, the preparation of fluid nutrient medium：Glucose, yeast juice, di(2-ethylhexyl)phosphate are added into seaweed extracted liquor obtained by step (A)
Hydrogen potassium, sodium chloride, ferrous sulfate, addition be respectively seaweed extracted liquor quality 2.5%, 1.3%, 0.04%, 0.03%,
0.002%, pH are adjusted to 7.4；
C, the preparation of zymotic fluid：Into the fermentation tank equipped with the fluid nutrient medium prepared by step (B), weight percent is inoculated with
Than the streptomyces microflavus after the activation for 2%, the thin yellow chain in 27 DEG C, 200rpm/min shaken cultivation 5d, obtained zymotic fluid
The CFU of mould is streptomyces microflavus >=2.0 × 109cfu/mL；
(2) preparation of liquid microbe fertilizer：
Calcium carbonate, magnesium sulfate, borax, ferric sulfate, manganese sulfate, zinc sulfate, copper sulphate, ammonium molybdate and step are weighed by weight
Suddenly streptomyces microflavus zymotic fluid obtained by (1), be well mixed, both liquid microbe fertilizer of the present invention.
Embodiment 2
A kind of liquid microbe fertilizer for preventing and treating cucumber fungal disease, include the raw material of following weight parts：Thin yellow strepto-
60 parts of fermented liquid, 65 parts of calcium carbonate, 40 parts of magnesium sulfate, 0.5 part of borax, 0.6 part of ferric sulfate, 0.2 part of manganese sulfate, zinc sulfate
0.001 part, 0.005 part of copper sulphate, 0.005 part of ammonium molybdate.
A kind of preparation method of above-mentioned liquid microbe fertilizer for preventing and treating cucumber fungal disease, comprises the following steps：
(1) preparation of streptomyces microflavus zymotic fluid：
A, the preparation of seaweed extracted liquor：320 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6 amount adds
Enter deionized water, heat enzyme deactivation, obtain suspension；The pH to 7.0 of suspension is adjusted, adds α~amylase, neutral proteinase, fibre
Plain enzyme, non-starch polysaccharide enzyme are tieed up, its addition is followed successively by 3%, 2%, 3%, the 1% of marine alga quality, is acted in ultrasonic assistant
Lower enzymolysis, 50 DEG C of hydrolysis temperature, ultrasonic power 300W, enzymolysis time 60min, is during which at the uniform velocity stirred using mixer；Enzymolysis
Enzyme deactivation, centrifugation, take supernatant, obtain seaweed extracted liquor after end；
B, the preparation of fluid nutrient medium：Glucose, yeast juice, di(2-ethylhexyl)phosphate are added into seaweed extracted liquor obtained by step (A)
Hydrogen potassium, sodium chloride, ferrous sulfate, addition be respectively seaweed extracted liquor quality 1%, 2%, 0.01%, 0.05%,
0.001%, pH are adjusted to 7.0；
C, the preparation of zymotic fluid：Into the fermentation tank equipped with the fluid nutrient medium prepared by step (B), weight percent is inoculated with
Than the streptomyces microflavus after the activation for 2%, the thin yellow chain in 32 DEG C, 160rpm/min shaken cultivation 6d, obtained zymotic fluid
The CFU of mould is streptomyces microflavus >=2.0 × 109cfu/mL；
(2) preparation of liquid microbe fertilizer：
Calcium carbonate, magnesium sulfate, borax, ferric sulfate, manganese sulfate, zinc sulfate, copper sulphate, ammonium molybdate and step are weighed by weight
Suddenly streptomyces microflavus zymotic fluid obtained by (1), be well mixed, both liquid microbe fertilizer of the present invention.
Embodiment 3
A kind of liquid microbe fertilizer for preventing and treating cucumber fungal disease, include the raw material of following weight parts：Thin yellow strepto-
65 parts of fermented liquid, 70 parts of calcium carbonate, 28 parts of magnesium sulfate, 0.7 part of borax, 0.7 part of ferric sulfate, 0.3 part of manganese sulfate, zinc sulfate
0.005 part, 0.004 part of copper sulphate, 0.003 part of ammonium molybdate.
A kind of preparation method of above-mentioned liquid microbe fertilizer for preventing and treating cucumber fungal disease, comprises the following steps：
(1) preparation of streptomyces microflavus zymotic fluid：
A, the preparation of seaweed extracted liquor：300 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6 amount adds
Enter deionized water, heat enzyme deactivation, obtain suspension；The pH to 7.0 of suspension is adjusted, adds α~amylase, neutral proteinase, fibre
Plain enzyme, non-starch polysaccharide enzyme are tieed up, its addition is followed successively by 4%, 1.5%, 2.5%, the 1.5% of marine alga quality, auxiliary in ultrasonic wave
Help effect is lower to digest, 60 DEG C of hydrolysis temperature, ultrasonic power 300W, enzymolysis time 40min, during which at the uniform velocity stirred using mixer
Mix；Enzymolysis terminates rear enzyme deactivation, centrifugation, takes supernatant, obtains seaweed extracted liquor；
B, the preparation of fluid nutrient medium：Glucose, yeast juice, di(2-ethylhexyl)phosphate are added into seaweed extracted liquor obtained by step (A)
Hydrogen potassium, sodium chloride, ferrous sulfate, addition be respectively seaweed extracted liquor quality 2%, 1.5%, 0.03%, 0.03%,
0.003%, pH are adjusted to 7.3；
C, the preparation of zymotic fluid：Into the fermentation tank equipped with the fluid nutrient medium prepared by step (B), weight percent is inoculated with
Than the streptomyces microflavus after the activation for 2%, the thin yellow chain in 29 DEG C, 230rpm/min shaken cultivation 5d, obtained zymotic fluid
The CFU of mould is streptomyces microflavus >=2.0 × 109cfu/mL；
(2) preparation of liquid microbe fertilizer：
Calcium carbonate, magnesium sulfate, borax, ferric sulfate, manganese sulfate, zinc sulfate, copper sulphate, ammonium molybdate and step are weighed by weight
Suddenly streptomyces microflavus zymotic fluid obtained by (1), be well mixed, both liquid microbe fertilizer of the present invention.
Embodiment 4
A kind of liquid microbe fertilizer for preventing and treating cucumber fungal disease, include the raw material of following weight parts：Thin yellow strepto-
70 parts of fermented liquid, 80 parts of calcium carbonate, 20 parts of magnesium sulfate, 0.6 part of borax, 1 part of ferric sulfate, 0.4 part of manganese sulfate, zinc sulfate 0.01
Part, 0.003 part of copper sulphate, 0.001 part of ammonium molybdate.
A kind of preparation method of above-mentioned liquid microbe fertilizer for preventing and treating cucumber fungal disease, comprises the following steps：
(1) preparation of streptomyces microflavus zymotic fluid：
A, the preparation of seaweed extracted liquor：270 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6 amount adds
Enter deionized water, heat enzyme deactivation, obtain suspension；The pH to 7.0 of suspension is adjusted, adds α~amylase, neutral proteinase, fibre
Plain enzyme, non-starch polysaccharide enzyme are tieed up, its addition is followed successively by 5%, 1%, 2%, the 2% of marine alga quality, is acted in ultrasonic assistant
Lower enzymolysis, 70 DEG C of hydrolysis temperature, ultrasonic power 300W, enzymolysis time 30min, is during which at the uniform velocity stirred using mixer；Enzymolysis
Enzyme deactivation, centrifugation, take supernatant, obtain seaweed extracted liquor after end；
B, the preparation of fluid nutrient medium：Glucose, yeast juice, di(2-ethylhexyl)phosphate are added into seaweed extracted liquor obtained by step (A)
Hydrogen potassium, sodium chloride, ferrous sulfate, addition be respectively seaweed extracted liquor quality 3%, 1%, 0.05%, 0.02%,
0.005%, pH are adjusted to 7.2；
C, the preparation of zymotic fluid：Into the fermentation tank equipped with the fluid nutrient medium prepared by step (B), weight percent is inoculated with
Than the streptomyces microflavus after the activation for 2%, the thin yellow chain in 28 DEG C, 260rpm/min shaken cultivation 4d, obtained zymotic fluid
The CFU of mould is streptomyces microflavus >=2.0 × 109cfu/mL；
(2) preparation of liquid microbe fertilizer：
Calcium carbonate, magnesium sulfate, borax, ferric sulfate, manganese sulfate, zinc sulfate, copper sulphate, ammonium molybdate and step are weighed by weight
Suddenly streptomyces microflavus zymotic fluid obtained by (1), be well mixed, both liquid microbe fertilizer of the present invention.
Embodiment 5
A kind of liquid microbe fertilizer for preventing and treating cucumber fungal disease, include the raw material of following weight parts：Thin yellow strepto-
100 parts of fermented liquid, 75 parts of calcium carbonate, 10 parts of magnesium sulfate, 1 part of borax, 0.8 part of ferric sulfate, 0.5 part of manganese sulfate, zinc sulfate
0.007 part, 0.007 part of copper sulphate, 0.002 part of ammonium molybdate.
A kind of preparation method of above-mentioned liquid microbe fertilizer for preventing and treating cucumber fungal disease, comprises the following steps：
(1) preparation of streptomyces microflavus zymotic fluid：
A, the preparation of seaweed extracted liquor：200 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6 amount adds
Enter deionized water, heat enzyme deactivation, obtain suspension；The pH to 7.0 of suspension is adjusted, adds α~amylase, neutral proteinase, fibre
Plain enzyme, non-starch polysaccharide enzyme are tieed up, its addition is followed successively by 4.5%, 1.8%, 2.7%, the 1.7% of marine alga quality, in ultrasonic wave
Digest, 65 DEG C of hydrolysis temperature, ultrasonic power 300W, enzymolysis time 45min, during which at the uniform velocity stirred using mixer under booster action
Mix；Enzymolysis terminates rear enzyme deactivation, centrifugation, takes supernatant, obtains seaweed extracted liquor；
B, the preparation of fluid nutrient medium：Glucose, yeast juice, di(2-ethylhexyl)phosphate are added into seaweed extracted liquor obtained by step (A)
Hydrogen potassium, sodium chloride, ferrous sulfate, addition be respectively seaweed extracted liquor quality 1.5%, 1.8%, 0.02%, 0.04%,
0.004%, pH are adjusted to 7.1；
C, the preparation of zymotic fluid：Into the fermentation tank equipped with the fluid nutrient medium prepared by step (B), weight percent is inoculated with
Than the streptomyces microflavus after the activation for 2%, the thin yellow chain in 30 DEG C, 180rpm/min shaken cultivation 6d, obtained zymotic fluid
The CFU of mould is streptomyces microflavus >=2.0 × 109cfu/mL；
(2) preparation of liquid microbe fertilizer：
Calcium carbonate, magnesium sulfate, borax, ferric sulfate, manganese sulfate, zinc sulfate, copper sulphate, ammonium molybdate and step are weighed by weight
Suddenly streptomyces microflavus zymotic fluid obtained by (1), be well mixed, both liquid microbe fertilizer of the present invention.
Comparative example 1
Streptomyces microflavus is not added in liquid fertilizer, includes the raw material of following weight parts：70 parts of calcium carbonate, magnesium sulfate 28
Part, 0.7 part of borax, 0.7 part of ferric sulfate, 0.3 part of manganese sulfate, 0.005 part of zinc sulfate, 0.004 part of copper sulphate, ammonium molybdate 0.003
Part.
The preparation method of aforesaid liquid fertilizer, comprises the following steps：
(1) preparation of seaweed extracted liquor：300 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6 amount
Deionized water is added, enzyme deactivation is heated, obtains suspension；Adjust suspension pH to 7.0, add α~amylase, neutral proteinase,
Cellulase, non-starch polysaccharide enzyme, its addition is followed successively by 4%, 1.5%, 2.5%, the 1.5% of marine alga quality, in ultrasonic wave
Digest, 60 DEG C of hydrolysis temperature, ultrasonic power 300W, enzymolysis time 40min, during which at the uniform velocity stirred using mixer under booster action
Mix；Enzymolysis terminates rear enzyme deactivation, centrifugation, takes supernatant, obtains seaweed extracted liquor；
(2) glucose, yeast juice, potassium dihydrogen phosphate, sodium chloride, sulfuric acid are added into seaweed extracted liquor obtained by step (1)
Ferrous iron, addition be respectively seaweed extracted liquor quality 3%, 1%, 0.05%, 0.02%, 0.005%, pH be adjusted to 7.2, obtain mixed
Close liquid A；
(3) calcium carbonate, magnesium sulfate, borax, ferric sulfate, manganese sulfate, zinc sulfate, copper sulphate, ammonium molybdate are weighed by weight
And step (2) obtained by mixed liquor A, be well mixed, both liquid fertilizer of the present invention.
Test example 1
The measure of technical indicator
According to the national standard (GB20287-2006) of microbial manure, above-described embodiment 1~5 and comparative example 1 are pressed in detection
Living bacteria count, miscellaneous bacteria rate, pH and innoxious index in the fertilizer sample of preparation.
Testing result：Testing result is as shown in table 1 below.
The technical indicator measurement result of table 1
After testing, a kind of liquid microbe fertilizer of preventing and treating cucumber fungal disease of the invention meets the state of microbial manure
Family's standard (GB20287-2006).Compared with comparative example 1, living bacteria count dramatically increases in embodiments of the invention 1~5, miscellaneous
Bacterium rate significantly reduces, and living bacteria count highest contained in embodiment 3, is 5.4 hundred million/milliliter, and miscellaneous bacteria rate is minimum, is 3.0%.
Living bacteria count is the main index of microbial manure product, illustrates that a kind of liquid of preventing and treating cucumber fungal disease of the present invention is micro-
Bio-feritlizer is had fine qualities, and the yield and quality of agricultural product can be significantly improved for agricultural production.
Test example 2
A kind of inhibitory action of the liquid microbe fertilizer of preventing and treating cucumber fungal disease of the present invention to cucumber disease fungus
The fertilizer sample prepared using mycelial growth rate method measure by above-described embodiment 1~5 and comparative example 1 is supplied 6 kinds
Try pathogen (Pseudoperonospora cubensis, Curcurbitaceae powdery mildew, melon single softgel shell powdery mildew, Fusarium oxysporum, Rhizoctonia solani Kuhn, sclerotium
Germ) inhibitory action.Fertilizer sample is diluted to 10,60,120,200 times with aseptic deionized water, is added to quantitative go out
It is cooled to after bacterium in 50 DEG C or so of PDA culture medium, fully shakes up, drug containing flat board is made.With add with sample equivalent it is sterile go from
Sub- water is control, and often processing is repeated 3 times.Beaten with diameter 5mm card punch and take well-grown stem, by stem mycelia downwards
It is inoculated in drug containing flat board center.25 DEG C of constant incubator culture a couple of days are positioned over after connecting strain, measure colony diameter, calculate bacterium
Silk growth inhibition ratio.The inspection result by the 3rd day, stop checking when bacterium colony length to plate edge is compareed.By following formula
Calculate bacteriostasis rate.
Bacteriostasis rate=[(control colony diameter-processing colony diameter)/(control colony diameter -5)] × 100%
Measurement result：Testing result is as shown in table 2 below.
Table 2 is to the inhibitory action for trying pathogen
As shown in Table 2, compared with comparative example 1, a kind of preventing and treating cucumber fungal disease of the preparation of the embodiment of the present invention 1~5
When liquid microbe fertilizer is diluted to 10 times, 60 times, 120 times, 200 times, comparative example 1 is far above to the bacteriostasis rate of 6 kinds of germs,
Fungistatic effect is notable.Wherein, when fertilizer sample is diluted to 10 times, 60 times, 100% is reached to the bacteriostasis rate of 6 kinds of germs；When
When fertilizer sample is diluted to 120 times, to Pseudoperonospora cubensis, Curcurbitaceae powdery mildew, melon single softgel shell powdery mildew inhibitory action compared with
By force, bacteriostasis rate is 89.2~91.5%, to the bacteriostasis rate of Fusarium oxysporum, Rhizoctonia solani Kuhn, hyphal cluster germ for 79.7~
82.7%；When fertilizer sample is diluted to 200 times, the suppression to Pseudoperonospora cubensis, Curcurbitaceae powdery mildew, melon single softgel shell powdery mildew
Bacterium rate is 69.4~72.8%, and the bacteriostasis rate to Fusarium oxysporum, Rhizoctonia solani Kuhn, hyphal cluster germ is 59.8~62.1%.
Result of the test shows that a kind of liquid microbe fertilizer of preventing and treating cucumber fungal disease of the invention can be prevented and treated effectively
Pseudoperonospora cubensis, Curcurbitaceae powdery mildew, melon single softgel shell powdery mildew, Fusarium oxysporum, Rhizoctonia solani Kuhn, hyphal cluster germ infect
Fungal disease, have a extensive future.
Test example 3
A kind of influence of the liquid microbe fertilizer of preventing and treating cucumber fungal disease of the present invention to cucumber yield and quality
Experiment is carried out in heliogreenhouse, is the close thorn in Xintai City for examination cucumber variety, experiment uses randomized complete-block design, often
Block experimental plot area is 3.5m2.Experiment sets 6 processing altogether, and processing 1 is that clear water compares, and processing 2~6 is by above-described embodiment 1
~5 liquid microbe fertilizer samples prepared, each processing are repeated 3 times.Processing 2~6 is in cucumber initial bloom stage, first fruiting period, Sheng Guo
Once, the initial bloom stage amount of spraying or pouring root amount are 1kg/ to the liquid microbe fertilizer that phase respectively sprays or prepared by pouring root embodiment 1~5
Mu, the first fruiting period amount of spraying or pouring root amount is 2.5kg/ mus, and the best fruiting period amount of spraying or pouring root amount are 3kg/ mus；Processing 1 is at the beginning of cucumber
Florescence, first fruiting period, the best fruiting period respectively sprays or pouring root equivalent clear water.Experimental plot others base manure, top dressing, irrigation and other fields
The conditions such as management are consistent.
Harvest and sample in the cucumber best fruiting period, and determine each processing cucumber yield (single melon weight, sugar-preserved gourd number and single plant yield) and
Quality (soluble sugar content, Vitamin C content and soluble protein content).Result of the test is as shown in table 3 below.
Influence of the table 3 to cucumber yield and quality
As shown in Table 3, compared with compareing clear water, a kind of preventing and treating cucumber fungal disease of the preparation of the embodiment of the present invention 1~5
Liquid microbe fertilizer can effectively improve cucumber yield, improve cucumber quality, wherein, the best results of embodiment 3 are clear with compareing
Water is compared, and cucumber list melon weight, sugar-preserved gourd number and single plant yield are respectively increased 32.31%, 73.52%, 81.82%, and soluble sugar contains
Amount, Vitamin C content and soluble protein content are respectively increased 89.44%, 72.18%, 54.79%.
Claims (8)
- A kind of 1. liquid microbe fertilizer for preventing and treating cucumber fungal disease, it is characterised in that the raw material including following weight parts： 50~100 parts of streptomyces microflavus zymotic fluid, 60~80 parts of calcium carbonate, 10~40 parts of magnesium sulfate, 0.5~1 part of borax, ferric sulfate 0.5~1 part, 0.1~0.5 part of manganese sulfate, 0.001~0.01 part of zinc sulfate, 0.001~0.007 part of copper sulphate, ammonium molybdate 0.001~0.005 part.
- A kind of 2. liquid microbe fertilizer for preventing and treating cucumber fungal disease according to claim 1, it is characterised in that including The raw material of following weight parts：60~70 parts of streptomyces microflavus zymotic fluid, 65~75 parts of calcium carbonate, 20~30 parts of magnesium sulfate, boron 0.6~0.8 part of sand, 0.6~0.8 part of ferric sulfate, 0.2~0.4 part of manganese sulfate, 0.003~0.007 part of zinc sulfate, copper sulphate 0.003~0.005 part, 0.002~0.004 part of ammonium molybdate.
- A kind of 3. liquid microbe fertilizer for preventing and treating cucumber fungal disease according to claim 2, it is characterised in that including The raw material of following weight parts：65 parts of streptomyces microflavus zymotic fluid, 70 parts of calcium carbonate, 28 parts of magnesium sulfate, 0.7 part of borax, sulfuric acid 0.7 part of iron, 0.3 part of manganese sulfate, 0.005 part of zinc sulfate, 0.004 part of copper sulphate, 0.003 part of ammonium molybdate.
- A kind of a kind of 4. system of the liquid microbe fertilizer of preventing and treating cucumber fungal disease as described in any one of claims 1 to 3 Preparation Method, comprise the following steps：(1) preparation of streptomyces microflavus zymotic fluid：A, the preparation of seaweed extracted liquor：200~400 parts of marine alga is weighed, is crushed, 100 mesh sieves are crossed, with feed liquid mass ratio 1：6 amount Deionized water is added, enzyme deactivation is heated, obtains suspension；Adjust suspension pH to 7.0, add α~amylase, neutral proteinase, Cellulase, non-starch polysaccharide enzyme, digest under ultrasonic assistant effect, during which at the uniform velocity stirred using mixer；Enzymolysis terminates Enzyme deactivation, centrifugation afterwards, take supernatant, obtain seaweed extracted liquor；B, the preparation of fluid nutrient medium：Added into seaweed extracted liquor obtained by step (A) glucose, yeast juice, potassium dihydrogen phosphate, Sodium chloride, ferrous sulfate, addition be respectively seaweed extracted liquor quality 1~3%, 1~2%, 0.01~0.05%, 0.02~ 0.05%th, 0.001~0.005%, pH is adjusted to 7.0~7.4；C, the preparation of zymotic fluid：Into the fermentation tank equipped with the fluid nutrient medium prepared by step (B), the thin Huang after inoculation activation Streptomycete, oscillation and fermentation cultivation, in obtained zymotic fluid the CFU of streptomyces microflavus for streptomyces microflavus >=2.0 × 109cfu/mL；(2) preparation of liquid microbe fertilizer：Calcium carbonate, magnesium sulfate, borax, ferric sulfate, manganese sulfate, zinc sulfate, copper sulphate, ammonium molybdate and step are weighed by weight (1) gained streptomyces microflavus zymotic fluid, it is well mixed, produces liquid microbe fertilizer of the present invention.
- 5. a kind of preparation method of liquid microbe fertilizer for preventing and treating cucumber fungal disease according to claim 4, it is special Sign is that α~amylase, neutral proteinase, cellulase, the addition of non-starch polysaccharide enzyme are followed successively by the step (1) 3~5%, 1~2%, 2~3%, the 1~2% of marine alga quality.
- 6. a kind of preparation method of liquid microbe fertilizer for preventing and treating cucumber fungal disease according to claim 4, it is special Sign is that enzymatic hydrolysis condition is in the step (1)：50~70 DEG C, ultrasonic power 300W of hydrolysis temperature, enzymolysis time be 30~ 60min。
- 7. a kind of preparation method of liquid microbe fertilizer for preventing and treating cucumber fungal disease according to claim 4, it is special Sign is that streptomyces microflavus inoculation percentage by weight is 1%~5% in the step (1).
- 8. a kind of preparation method of liquid microbe fertilizer for preventing and treating cucumber fungal disease according to claim 4, it is special Sign is that fermentation culture conditions are in the step (1)：28~32 DEG C, 4~6d of incubation time of cultivation temperature, shaken cultivation turn Speed is 160~260rpm/min.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108586154A (en) * | 2018-07-11 | 2018-09-28 | 合肥百绿盛农业科技有限公司 | A kind of green cucumber dedicated fertilizer and preparation method thereof |
| CN108715567A (en) * | 2018-07-27 | 2018-10-30 | 安龙县神农绿野种养殖农民专业合作社 | A kind of cucumber fertilizer special for organic and preparation method thereof |
| CN110036855A (en) * | 2019-05-23 | 2019-07-23 | 申琳 | A method of regulation Sweet Potato For High. yield Production with it is high-quality unified |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106866270A (en) * | 2017-03-25 | 2017-06-20 | 广州聚禅现代农业研究院有限公司 | A kind of liquid microbe fertilizer of the changing food waste into resources production containing streptomyces microflavus |
-
2017
- 2017-08-21 CN CN201710722619.7A patent/CN107353121A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106866270A (en) * | 2017-03-25 | 2017-06-20 | 广州聚禅现代农业研究院有限公司 | A kind of liquid microbe fertilizer of the changing food waste into resources production containing streptomyces microflavus |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108586154A (en) * | 2018-07-11 | 2018-09-28 | 合肥百绿盛农业科技有限公司 | A kind of green cucumber dedicated fertilizer and preparation method thereof |
| CN108715567A (en) * | 2018-07-27 | 2018-10-30 | 安龙县神农绿野种养殖农民专业合作社 | A kind of cucumber fertilizer special for organic and preparation method thereof |
| CN110036855A (en) * | 2019-05-23 | 2019-07-23 | 申琳 | A method of regulation Sweet Potato For High. yield Production with it is high-quality unified |
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Application publication date: 20171117 |