CN107348230A - A kind of preparation method of multifunctional microbial fish guano - Google Patents
A kind of preparation method of multifunctional microbial fish guano Download PDFInfo
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Abstract
The present invention relates to a kind of preparation method of multifunctional microbial fish guano, it comprises the following steps：S. cervisiae containing recombined Lactoferricin B plasmid is inoculated into fluid nutrient medium, is forwarded to after 10~24h of concussion and cultivate in fresh culture, treats OD60060~72h of fermentation cylinder for fermentation is forwarded between 1.5~2.5；After end, zymotic fluid is spray-dried with spray dryer, obtains Bovine lactoferricin yeast powder；Fermentation Cottonseed Meal, beast bone meal, maize leaves powder, hoof rubber powder, oyster shell whiting, water are well mixed with complex microorganism, are put into fermentation vat material A of fermenting to obtain, is placed in drying chamber and places 2~5 days, pulverize and sieve；Material A after crushing and Bovine lactoferricin yeast powder, trace element, citric acid are mixed into obtain material B on mixer；By material B with pressure be 2~4.5 kg of vapor it is quenched to temperature be 60~66 DEG C, pelletized with granulator, microbial fish guano of the invention provides abundant nutrient raw material for cultured product, improves water quality, increases the dissolved oxygen content in water.
Description
Technical field
The invention belongs to aquaculture field, and in particular to a kind of preparation method of multifunctional microbial fish guano.
Background technology
Traditional aqiuculrue will apply fertilizer as being a farmer in China, but for breeding fish, the purpose of fertilising is mainly
Increase the fertile degree of water, promote the growth and breeding of various bait biologies, more foodstuffs are provided for cultured fishes.Existing fish
Although fertilizer is lasting using rear fertilizer efficiency, substantial amounts of oxygen is consumed when decomposing slowly, and decomposing, easily causes aquatic products to move using improper
The hypoxia death of thing.Effective microorganism preparation has obvious facilitation effect for water surface fertilising, same with the special fish guano of aquatic products
When use, collaboration can be played a part of.Some products in the market are also to be made according to depot fertilizer and short-acting fertilizer compounding
, the biological feed required for fish is produced by promoting the planktonic organism in water, and high--yielding fish pond is raised by full price fish
Expect to feed fish.The density of fish is very large in pond, so that the speed of growth of planktonic organism is less than fish food biology
The speed of bait, so, final planktonic organism can not normal growth.It is a large amount of to apply meetings and existing fish guano function is more single
Polluted water, it is impossible to suppress the flourish of pathogen in water body, therefore, lack effective preventing and treating to fish disease；Meanwhile it is applied with
Fertilizer is stated in fishpond, water temperature is influenceed greatly by ambient weather change, such as " cold spell in later spring ", and water quality is unstable, and seed is difficult to fit
Should, emergency reaction is strong.
The content of the invention
In order to solve the above technical problems, the invention provides a kind of preparation method of multi-functional fish guano, by adding cow's milk
The nutritional ingredient such as ferritin peptide yeast powder and utilization compound microorganism ferments fermentation Cottonseed Meal so that the fish guano of preparation can be used for
The raising of fry raising, fingerling, eel kind, shrimp seedling, juvenile crab etc. in pond, reservoir or lake；On the one hand, Bovine lactoferricin is peculiar
Bactericidal action and metabolism caused by various enzymes the harmful bacteria in fishpond can be killed, reduce disease probability；The opposing party
Face, there is provided abundant nutrient raw material, improve water quality, increase the dissolved oxygen content in water.
To achieve these goals, the present invention is achieved by the following technical programs：
A kind of preparation method of multifunctional microbial fish guano, comprises the following steps：
(1) S. cervisiae containing recombined Lactoferricin B plasmid is inoculated into 100~500ml fluid nutrient mediums
In, 180~250rpm concussion and cultivates, 10~24h in 28~35 DEG C of constant incubators；
(2) above-mentioned bacterium solution is forwarded in 1L~2.5L fresh liquid culture mediums, 180~250rpm concussion and cultivates, treated
OD600It is forwarded between 1.5~2.5 by the 60~72h that in the fermentation tank of sterilizing, fermented in 30~35 DEG C；
(3) after fermentation ends, by tank temperature rise to 90~98 DEG C of holding 10min, natural cooling, fermentation is collected after cooling
Liquid, and be spray-dried with spray dryer, produce Bovine lactoferricin yeast powder；
(4) by 14~24 parts of fermentation Cottonseed Meal, 12~19 parts of beast bone meal, 7~11 parts of maize leaves powder, 5~9 parts
Hoof rubber powder, 3~7 parts of oyster shell whiting, 30~43 parts of water and complex microorganism are mixed and stirred for uniformly, being put into fermentation vat certainly
So fermentation obtains material A in 5~10 days, is during which stirred 1 time every 1 day；
(5) material A is placed in drying chamber and placed 2~5 days at 52~83 DEG C, pulverized and sieved；
(6) by the material A after crushing and 23~36 parts of lactoferricin yeast powder, 0.5~1.5 part of trace element,
11~20 parts of citric acid is sufficiently stirred on mixer mixes to obtain material B；
(7) by material B with pressure be 2~4.5 kg of vapor it is quenched to temperature be 60~66 DEG C, pelletized with granulator, it is cold
But pack preserves afterwards, produces above-mentioned fish guano finished product；
Preferably, the complex microorganism includes the strain of following parts by weight：5~11 parts of bacillus azotobacter, wine laterite
Brown 6~10 parts of streptomycete, 3~9 parts of Lactobacillus plantarum, 5~10 parts of candida utili；
Preferably, the complex microorganism includes the strain of following parts by weight：6 parts of bacillus azotobacter, the brown chain of wine laterite
8 parts of mould, 7 parts of Lactobacillus plantarum, 8 parts of candida utili；
Preferably, the fermentation Cottonseed Meal is preferably 20 parts, beast bone meal is preferably 15 parts, maize leaves powder is preferably 29 parts,
Hoof rubber powder is preferably 27 parts, oyster shell whiting is preferably 25 parts, water is preferably 36 parts；
Preferably, the Bovine lactoferricin yeast powder is preferably 32 parts, micro- preferably 1 part, citric acid is preferred
For 17 parts；
Preferably, the trace element is for containing the one or more in manganese, aluminium, copper, boron, molybdenum and zinc inorganic salts；
The upper fluid nutrient medium includes the raw material of following parts by weight：10~20 parts of cerelose, industrial yeast cream 5~
12 parts, 10~20 parts of industrial proteins peptone, 5~10 parts of salt, 2~6 parts of dipotassium hydrogen phosphate, 3~7 parts of sodium dihydrogen phosphate, water 60~
78 parts；
Preferably, the inoculum concentration of the inoculation is 1%~10%.
Beneficial effects of the present invention are：
1st, high activity, the Bovine lactoferricin of high nutrition are obtained using fermentation engineering, it can suppress in water body
Pathogen and the growth and breeding of harmful algae, and can, which enters in the alimentary canal of fish, balances micro-ecological environment, and enhancing cellular immunity is anti-
Should, resistance is improved, realizes the maximization of culture benefit.
2nd, according to the nutritional need of aquifer cultivation product and the regulatory mechanism of water body micro-ecological environment, multiple-microorganism is utilized
Fermentation biological fish manure, abundant nutrition is provided for aquatic livestock, phytoplankton and zooplankter, promotes the life of cultured product
Long and development；Bacillus azotobacter can be in degradation water ammoniacal nitrogen, nitrite, the harmful substance such as hydrogen sulfide, produce simultaneously
Oxygen, promote the growth and breeding of beneficial algae and planktonic organism bait in water body, the natural of abundance is provided for aquiculture animal
Biological feed.Lactobacillus plantarum can by with pathogen to limiting nutrient element ability compete, to suppress the growth of pathogen,
The composition of intestinal microecology is adjusted, forms biological containment.Meanwhile pass through metabolite lactic acid, bacterium caused by its metabolism
The effect to other bacteriums of element and biacetyl, adjusts the relation between flora, maintain and ensure the combination of flora optimal vigor with
And the stabilization of this combination, prevent colonizing and invading for pathogenic bacteria, antagonism pathogenic bacteria and harmful microbe growth and its poison
The adhesion of element.
3rd, by compound microorganism ferments macromolecule protein raw material, contained using fish guano made from formula technique containing more
Protein, carbohydrate, amino acid and multivitamin, provided for the aquatic livestocks such as fish, phytoplankton and zooplankter abundant
Nutrition, improve water quality, increase the dissolved oxygen content in water, and preparation method is simple, is easy to large-scale production.
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with specific embodiment pair
The present invention is described in detail.It should be pointed out that creative work premise is not being made to those of ordinary skill in the art
Lower obtained every other embodiment, belongs to the scope of protection of the invention.
Embodiment 1：
A kind of preparation method of multifunctional microbial fish guano, comprises the following steps：
(1) 3% S. cervisiae of (v/v) containing recombined Lactoferricin B plasmid is inoculated into 100ml Liquid Cultures
In base, the 190rpm concussion and cultivates 15h in 35 DEG C of constant incubators；
(2) above-mentioned bacterium solution is forwarded in 1.5L fresh liquid culture mediums, 190rpm concussion and cultivates, treats OD6001.7 it
Between be forwarded in the fermentation tank by sterilizing, in 35 DEG C ferment 62h；
(3) after fermentation ends, by tank temperature rise to 98 DEG C of holding 10min, natural cooling, zymotic fluid is collected after cooling, and
It is spray-dried with spray dryer, produces Bovine lactoferricin yeast powder；
(4) by 14 parts of fermentation Cottonseed Meal, 16 parts of beast bone meal, 10 parts of maize leaves powder, 7 parts of hoof rubber powder, 4 parts of shellfish
Shell powder, 30 parts of water and 5 parts of bacillus azotobacter, 6 parts of the brown streptomycete of wine laterite, 3 parts of Lactobacillus plantarum, 10 parts of production
Protein Candida is mixed and stirred for uniformly, being put into spontaneous fermentation in fermentation vat and obtaining material A in 5 days, during which stirred 1 time every 1 day；
(5) material A is placed in drying chamber and placed 2 days at 63 DEG C, pulverized and sieved；
(6) by lactoferricin yeast powder of the material A after crushing with 28 parts, 0.2 MnSO4Part, 0.3 part of AlCl3、
0.1 part of CuCl2, 0.4 part of H3BO4, 11 parts of citric acid is sufficiently stirred on mixer and mixes to obtain material B；
(7) by material B with pressure be 2 kg of vapor it is quenched to temperature be 63 DEG C, pelletized with granulator, pack and protect after cooling
Deposit, produce above-mentioned fish guano finished product.
Aforesaid liquid culture medium includes the raw material of following parts by weight：14 parts of cerelose, 7 parts of industrial yeast cream, industry
14 parts of peptone, 8 parts of salt, 4 parts of dipotassium hydrogen phosphate, 5 parts of sodium dihydrogen phosphate, 78 parts of water.
Embodiment 2：
A kind of preparation method of multifunctional microbial fish guano, comprises the following steps：
(1) 1% S. cervisiae of (v/v) containing recombined Lactoferricin B plasmid is inoculated into 200ml Liquid Cultures
In base, the 180rpm concussion and cultivates 24h in 28 DEG C of constant incubators；
(2) above-mentioned bacterium solution is forwarded in 1L fresh liquid culture mediums, 180rpm concussion and cultivates, treats OD600Between 1.5
It is forwarded in the fermentation tank by sterilizing, ferment 60h in 30 DEG C；
(3) after fermentation ends, by tank temperature rise to 94 DEG C of holding 10min, natural cooling, zymotic fluid is collected after cooling, and
It is spray-dried with spray dryer, produces Bovine lactoferricin yeast powder；
(4) by 19 parts of fermentation Cottonseed Meal, 9 parts of beast bone meal, 7 parts of maize leaves powder, 9 parts of hoof rubber powder, 3 parts of shell
Powder, 30 parts of water and 8 parts of bacillus azotobacter, 6 parts of the brown streptomycete of wine laterite, 6 parts of Lactobacillus plantarum, 5 parts of production protein
Candida is mixed and stirred for uniformly, being put into spontaneous fermentation in fermentation vat and obtaining material A in 7 days, during which stirred 1 time every 1 day；
(5) material A is placed in drying chamber and placed 4 days at 83 DEG C, pulverized and sieved；
(6) by lactoferricin yeast powder of the material A after crushing with 23 parts, 0.3 part of AlCl3, 0.4 part of CuCl2、
0.1 part of H3BO4, 0.1 part of NaMoO4, 35 parts of citric acid is sufficiently stirred on mixer and mixes to obtain material B；
(7) by material B with pressure be 4.5 kg of vapor it is quenched to temperature be 60 DEG C, pelletized with granulator, packed after cooling
Preserve, produce above-mentioned fish guano finished product.
Aforesaid liquid culture medium includes the raw material of following parts by weight：10 parts of cerelose, 5 parts of industrial yeast cream, industry
20 parts of peptone, 5 parts of salt, 3 parts of dipotassium hydrogen phosphate, 6 parts of sodium dihydrogen phosphate, 65 parts of water.
Embodiment 3：
A kind of preparation method of multifunctional microbial fish guano, comprises the following steps：
(1) 10% S. cervisiae of (v/v) containing recombined Lactoferricin B plasmid is inoculated into 500ml Liquid Cultures
In base, the 250rpm concussion and cultivates 10h in 32 DEG C of constant incubators；
(2) above-mentioned bacterium solution is forwarded in 2L fresh liquid culture mediums, 250rpm concussion and cultivates, treats OD600Between 2.5
It is forwarded in the fermentation tank by sterilizing, ferment 60h in 33 DEG C；
(3) after fermentation ends, by tank temperature rise to 90 DEG C of holding 10min, natural cooling, zymotic fluid is collected after cooling, and
It is spray-dried with spray dryer, produces Bovine lactoferricin yeast powder；
(4) by 24 parts of fermentation Cottonseed Meal, 12 parts of beast bone meal, 11 parts of maize leaves powder, 5 parts of hoof rubber powder, 7 parts of shellfish
Shell powder, 43 parts of water and 11 parts of bacillus azotobacter, 10 parts of the brown streptomycete of wine laterite, 9 parts of Lactobacillus plantarum, 6 parts
Candida utili is mixed and stirred for uniformly, being put into spontaneous fermentation in fermentation vat and obtaining material A in 7 days, during which stirred 1 every 1 day
It is secondary；
(5) material A is placed in drying chamber and placed 5 days at 52 DEG C, pulverized and sieved；
(6) by lactoferricin yeast powder of the material A after crushing with 36 parts, 0.4 part of AlCl3, 0.4 part of CuCl2、
0.5 part of H3BO4, 0.2 part of NaMoO4, 20 parts of citric acid is sufficiently stirred on mixer and mixes to obtain material B；
(7) by material B with pressure be 2.5 kg of vapor it is quenched to temperature be 66 DEG C, pelletized with granulator, packed after cooling
Preserve, produce above-mentioned fish guano finished product.
Aforesaid liquid culture medium includes the raw material of following parts by weight：20 parts of cerelose, 12 parts of industrial yeast cream, industry
10 parts of peptone, 10 parts of salt, 2 parts of dipotassium hydrogen phosphate, 3 parts of sodium dihydrogen phosphate, 78 parts of water.
Embodiment 4：
A kind of preparation method of multifunctional microbial fish guano, comprises the following steps：
(1) 5% S. cervisiae of (v/v) containing recombined Lactoferricin B plasmid is inoculated into 300ml Liquid Cultures
In base, the 220rpm concussion and cultivates 18h in 30 DEG C of constant incubators；
(2) above-mentioned bacterium solution is forwarded in 1.5L fresh liquid culture mediums, 220rpm concussion and cultivates, treats OD6002.0 it
Between be forwarded in the fermentation tank by sterilizing, in 32 DEG C ferment 60h；
(3) after fermentation ends, by tank temperature rise to 96 DEG C of holding 10min, natural cooling, zymotic fluid is collected after cooling, and
It is spray-dried with spray dryer, produces Bovine lactoferricin yeast powder；
(4) by 20 parts of fermentation Cottonseed Meal, 15 parts of beast bone meal, 9 parts of maize leaves powder, 7 parts of hoof rubber powder, 5 parts of shellfish
Shell powder, 36 parts of water and 6 parts of bacillus azotobacter, 8 parts of the brown streptomycete of wine laterite, 7 parts of Lactobacillus plantarum, 8 parts of production
Protein Candida is mixed and stirred for uniformly, being put into spontaneous fermentation in fermentation vat and obtaining material A in 7 days, during which stirred 1 time every 1 day；
(5) material A is placed in drying chamber and placed 3 days at 65 DEG C, pulverized and sieved；
(6) by lactoferricin yeast powder of the material A after crushing with 36 parts, 0.4 part of AlCl3, 0.4 part of CuCl2、
0.5 part of H3BO4, 0.2 part of NaMoO4, 17 parts of citric acid is sufficiently stirred on mixer and mixes to obtain material B；
(7) by material B with pressure be 3 kg of vapor it is quenched to temperature be 63 DEG C, pelletized with granulator, pack and protect after cooling
Deposit, produce above-mentioned fish guano finished product.
Aforesaid liquid culture medium includes the raw material of following parts by weight：13 parts of cerelose, 6 parts of industrial yeast cream, industry
13 parts of peptone, 7 parts of salt, 3 parts of dipotassium hydrogen phosphate, 5 parts of sodium dihydrogen phosphate, 66 parts of water.
Application examples 1：
Culture experiment is carried out in Hefei City, Anhui Province Chaohu Jiang Ka seedling breedings Co., Ltd, and natural water area temperature is 25
~30 DEG C, pH value 8~8.5.Mark seedling facility has 6.4 × 12.5 × 2.1 cement pit numbers mouthful, 1Kw oxygen increasing pump number platforms.By bottom of pond and pond
Wall outwash, bleaching powder sterilization, 3 times test chlorine residue are rinsed, it is qualified.
1 blank group of Setup Experiments, 1 positive controls (commercially available organic fish guano), 4 test groups (launch this hair respectively
Fish guano made from bright 1~4 group of embodiment), the parallel pond of every group of setting.Put before seedling 7 days, positive controls and test group per pond according to
Every mu of 3kg applies corresponding fish guano, is preferably applied fertilizer at fine day 8~12.Take cultivation water to be observed after 3 days, the results are shown in Table 1.
The fertilization effect of the microbial fish guano of table 1
Apply fertilizer the 5th day and start to put seedling, 10000 tail mandarin fish seedlings are put per Chi Pinjun, put after seedling and applied fertilizer once every 10~15 days,
Dose is every mu of 3kg, every group routinely breeding way carry out daily management.
Feed the 15th day, blank group morbidity, other fishponds are normal.Cultivate the 30th day, receive fish and divide pond, calculate survival rate and increasing
Long rate, is shown in Table 2.
The mandarin fish survival and growth situation of table 2
This experiment shows that the fast-growth of fish can not only be promoted by adding the microbial fish guano of the present invention, can also be carried
The quality and quality of high fish, but also increase the beneficial microbe in water body, can effective preventing and curing of fish disease generation, be a kind of
Cost-effective cultural method.Ox such as ferritin peptide has good biocompatibility, gastrointestinal tract mucous compatibility, water body in itself
Middle pathogen and the growth and breeding of harmful algae, and can, which enters in the alimentary canal of fish, balances micro-ecological environment, strengthens cellular immunity
Reaction, resistance is improved, fertilizer efficiency time extends more than 3 months, effectively suppresses the increment of animal pathogen, improves the effect of the producer
Benefit.
Application examples 2
Sample plot point is selected in Bozhou City watchtower city abundance of water source cultivation factory, and cultivating pool randomly selects 5 specification identical ponds,
Before experiment, pond is by cleaning up the pond, shining pool processing, and breeding water is using the clean sea water after husky filter well filtering, no harmful organisms.
1 blank group of Setup Experiments, 1 positive controls (commercially available organic fish guano), 4 test groups (launch this hair respectively
Microbial fish guano made from bright 1~4 group of embodiment), the parallel pond of every group of setting.Put before seedling 5 days, positive controls and test group are every
Corresponding fish guano is applied in pond according to every mu of 2kg, is preferably applied fertilizer at fine day 12~16.Cultivation water is taken to be observed after 3 days, control group
It is in Green Water based on green alga, test group brown water based on diatom, chrysophyceae.Apply fertilizer the 6th day and occur starting to put with ovum wheel animalcule
Seedling, the tail of silver carp 10000 is put per pond.Routinely breeding way carries out daily management, puts after seedling and was applied fertilizer once every 7~10 days.
Raise the 20th and 26 day, the control group morbidity row pool, other fishponds are normal.Cultivation 110 days, fish is received, calculates survival rate,
It is shown in Table 3.
The silver carp survival and growth situation of table 3
This experiment shows, the life of yellow green and brown algae in water body can be improved using the microbial fish guano of the present invention
Object amount, the biomass with ovum wheel animalcule is increased in water body, and abundant natural bait is provided for cultivated animals, is effectively saved
Aquaculture cost.And the dissolved oxygen rise in cultivation water, cultivated animals not susceptible disease, effectively improve breeding efficiency.
The present invention is described in detail with reference to the foregoing embodiments, it will be understood by those within the art that：
It can still modify to the technical scheme described in foregoing embodiments, or which part technical characteristic is carried out etc.
With replacement；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technical scheme
Spirit and scope.
Claims (8)
- A kind of 1. preparation method of multifunctional microbial fish guano, it is characterised in that：Comprise the following steps：(1) S. cervisiae containing recombined Lactoferricin B plasmid is inoculated into 100~500ml fluid nutrient mediums, in 180~250rpm concussion and cultivates, 10~24h in 28~35 DEG C of constant incubators；(2) above-mentioned bacterium solution is forwarded in 1L~2.5L fresh liquid culture mediums, 180~250rpm concussion and cultivates, treats OD600 It is forwarded between 1.5~2.5 in the fermentation tank by sterilizing, ferment 60~72h in 30~35 DEG C；(3) after fermentation ends, by tank temperature rise to 90~98 DEG C of holding 10min, natural cooling, zymotic fluid is collected after cooling, and It is spray-dried with spray dryer, produces Bovine lactoferricin yeast powder；(4) by 14~24 parts of fermentation Cottonseed Meal, 12~19 parts of beast bone meal, 7~11 parts of maize leaves powder, 5~9 parts of hoof glue Powder, 3~7 parts of oyster shell whiting, 30~43 parts of water and complex microorganism are mixed and stirred for uniformly, being put into fermentation vat and sending out naturally Ferment obtains material A in 5~10 days, is during which stirred 1 time every 1 day；(5) material A is placed in drying chamber and placed 2~5 days at 52~83 DEG C, pulverized and sieved；(6) by the material A after crushing and 23~36 parts of lactoferricin yeast powder, 0.5~1.5 part of trace element, 11~ 20 parts of citric acid is sufficiently stirred on mixer mixes to obtain material B；(7) by material B with pressure be 2~4.5 kg of vapor it is quenched to temperature be 60~66 DEG C, pelletized with granulator, after cooling Pack preserves, and produces above-mentioned fish guano finished product.
- 2. the preparation method of fish guano according to claim 1, it is characterised in that：The complex microorganism includes following weight The strain of part：6~10 parts of the brown streptomycete of 5~11 parts of bacillus azotobacter, wine laterite, 3~9 parts of Lactobacillus plantarum, Candida utilis 5~10 parts of yeast.
- 3. the preparation method of fish guano according to claim 1, it is characterised in that：The complex microorganism includes following weight The strain of part：8 parts of the brown streptomycete of 6 parts of bacillus azotobacter, wine laterite, 7 parts of Lactobacillus plantarum, 8 parts of candida utili.
- 4. the preparation method of fish guano according to claim 1, it is characterised in that：The fermentation Cottonseed Meal is preferably 20 parts, Beast bone meal is preferably 15 parts, maize leaves powder is preferably 9 parts, hoof rubber powder is preferably 7 parts, oyster shell whiting is preferably 5 parts, water is preferably 36 Part.
- 5. the preparation method of fish guano according to claim 1, it is characterised in that：The Bovine lactoferricin yeast powder is preferred For 32 parts, trace element be preferably 1 part, citric acid be preferably 17 parts.
- 6. the preparation method of fish guano according to claim 1, it is characterised in that：It is described trace element be for containing manganese, aluminium, One or more in copper, boron, molybdenum and zinc inorganic salts.
- 7. the preparation method of fish guano according to claim 1, it is characterised in that：The fluid nutrient medium includes following weight The raw material of part：10~20 parts of cerelose, 5~12 parts of industrial yeast cream, 10~20 parts of industrial proteins peptone, 5~10 parts of salt, 2~6 parts of dipotassium hydrogen phosphate, 3~7 parts of sodium dihydrogen phosphate, 60~78 parts of water.
- 8. the preparation method of fish guano according to claim 1, it is characterised in that：The inoculum concentration of the inoculation be 1%~ 10%.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116058439A (en) * | 2022-12-27 | 2023-05-05 | 珠海市德海生物科技有限公司 | Feed additive for replacing antibiotics and raw fish feed prepared by using feed additive |
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| CN116058439A (en) * | 2022-12-27 | 2023-05-05 | 珠海市德海生物科技有限公司 | Feed additive for replacing antibiotics and raw fish feed prepared by using feed additive |
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Application publication date: 20171117 |