CN107347753A - A kind of overwintering cultivation method for propagating leech artificially - Google Patents

A kind of overwintering cultivation method for propagating leech artificially Download PDF

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Publication number
CN107347753A
CN107347753A CN201710779134.1A CN201710779134A CN107347753A CN 107347753 A CN107347753 A CN 107347753A CN 201710779134 A CN201710779134 A CN 201710779134A CN 107347753 A CN107347753 A CN 107347753A
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winter
surviving
leech
casing
mesh bag
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CN201710779134.1A
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龚元
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Jingzhou Public Kang Biotechnology Co Ltd
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Jingzhou Public Kang Biotechnology Co Ltd
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Priority to CN201710779134.1A priority Critical patent/CN107347753A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/40Culture of aquatic animals of annelids, e.g. lugworms or Eunice

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The present invention relates to a kind of overwintering cultivation method for propagating leech artificially, belong to hirudiniculture technical field.It is characterized in:By 8 method and steps of the invention, it will survive the winter casing and mesh bag of surviving the winter combine moistening paddy soils and realizes that the high density of leech is survived the winter cultivation.The survive the winter top of casing is open type case mouth, and the bottom four corners for casing of surviving the winter are provided with pulley, and hole is uniformly made on box bottom of surviving the winter;Mesh bag of surviving the winter is made up of carriage, nylon net cloth, slide fastener, and mesh bag of surviving the winter is adapted to casing of surviving the winter to be jointly mounted to survive the winter in casing.Method is simple, and leech survival rate is high, and cost is low, is adapted to batch production leech to survive the winter cultivation;Cross-infection is avoided, leech disease incidence of surviving the winter is extremely low, effectively prevents leech high-volume death of surviving the winter.Labour is saved, is easy to manage.It is big to solve existing indoor leech cultivation area occupied of surviving the winter, launches that leech density is low, and management trouble, leech weight-loss ratio height of surviving the winter, yield is low, it is difficult to meets that high density is survived the winter cultivation the problem of requiring.

Description

A kind of overwintering cultivation method for propagating leech artificially
Technical field
The present invention relates to a kind of overwintering cultivation method for propagating leech artificially, belong to hirudiniculture technical field.
Background technology
With the increase year by year for the amount of fishing, wild leech population quantity is increasingly reduced, and artificial farming leech is imperative.Mesh Preceding hirudiniculture is survived the winter, and mainly carries out cold-proof cultivation using outdoor furrow, pond, cement pit and indoor paddy soils.It is outdoor The survival rate that pond, cement is pool cultivated to survive the winter is low, outdoor furrow and indoor paddy soils survive the winter cultivation survival rate it is higher, but take Area is big, and dispensing leech density is low, management trouble, it is impossible to meets that high density survives the winter to cultivate and requires.How to realize high density launch, High-survival rate, management is convenient, and simple to operate, cost is low, improves the cultivation leech that survives the winter of yield, is one and urgently to be resolved hurrily asks Topic.
The content of the invention
It is an object of the present invention to it is directed to above-mentioned the deficiencies in the prior art, there is provided a kind of cultural method is simple, manager Just, it is adapted to batch production high density to survive the winter cultivation, cultivating condition of surviving the winter is controllable with environment, leech survival rate height, and quality of finished product good is foster It is low to grow cost, saves labour, effectively improves the overwintering cultivation method of the artificial farming leech of cultivation yield.
The present invention is to realize above-mentioned purpose by the following technical solutions:
A kind of overwintering cultivation method for propagating leech artificially, it is characterised in that:It is completed by the steps:
1)Need disposably to clean survive the winter casing and mesh bag of surviving the winter first, and at least sterilized with potassium permanganate 1 time, while take paddy soils Cross 40 mesh sieves;
2)Mesh bag of surviving the winter is inserted in each casing of surviving the winter, is then mated formation in mesh bag of surviving the winter into paddy soils after treatment, paving Dress thickness is 20~25cm;
3)From specification is identical, the leech of body weight error≤0.5g/ bars as leech individual of surviving the winter, 7 days before dispensing, leech is put Enter into the interior feed box set up defences and escape mesh bag, by air-conditioning by temperature control at 25 DEG C~30 DEG C, by 5~8 times of leech gross weight Fresh ox blood bowel lavage after launch and disposably fed into feed box, feeding time ensures all leech in 30~40min All feds to appetite;Feed box and casing are intended to sterilize in advance;Feeding takes out casing after terminating, then clean leech and feed box dry Only, then by fed to appetite and the leech cleaned up it is put into casing of surviving the winter;
4)Casing proper alignment will be survived the winter in culturing chamber, survive the winter and people's bit manipulation space is reserved between casing and casing of surviving the winter;Often The top of individual casing of surviving the winter correspondingly is provided with inlet channel, and inlet channel is connected with water storage temperature-controlling system;
5)Cultivation indoor temperature is maintained at 4 DEG C~10 DEG C, and ventilated, casing of surviving the winter is interior according to the close of 2500~3000/case Degree launches leech, is controlled temperature of cultivation at 8 DEG C~12 DEG C by water storage temperature-controlling system, during surviving the winter 5 months, day in culturing chamber The temperature difference is no more than 2, keep cultivation indoor environment cleaning clean, while breeding water body clean transparent is kept, leech initially enters more Winter cultivates the phase;
6)After leech enters the cultivation phase of surviving the winter, have to check for daily once, discovery there are dead individuals to pick in time;
7)Every 7~10 days with inlet channel to casing water drenching one time of surviving the winter, ensure paddy soil humidity in 45%~55% section, Casing of surviving the winter gently is loosened the soil once with diameter 2cm waddy simultaneously, but not stir leech;
8)After the cultivation phase of surviving the winter of leech terminates, statistics survives bar number, weighs the weight per case leech, calculates survival rate and relative Weight-loss ratio, survival rate to more than 90%, relative weight loss rate are less than 6%;
The top of described casing of surviving the winter is open type case mouth, and the bottom four corners for casing of surviving the winter are provided with pulley, casing bottom of surviving the winter Hole is uniformly made on plate;Mesh bag of surviving the winter is made up of carriage, nylon net cloth, slide fastener, and nylon net cloth is enclosed on carriage Composition is survived the winter mesh bag, and mesh bag of surviving the winter is adapted to casing of surviving the winter, and mesh bag of surviving the winter is jointly mounted to survive the winter in casing, the top for mesh bag of surviving the winter Portion is provided with slide fastener;The survive the winter bottom of casing is mated formation and has paddy soils;Survive the winter the liftoff 10cm of pulley of casing, casing height of surviving the winter 60cm, long 100cm, wide 100cm, hole diameter 2cm;Mesh bag of surviving the winter high 50cm, long 100cm, wide 100cm;Survive the winter the net of mesh bag Hole is 60 mesh.
The beneficial effect of the present invention compared with prior art is:
By 8 steps of the invention, will survive the winter casing and mesh bag of surviving the winter combine wet the overwintering cultivation method of the artificial farming leech Moisten paddy soils, successfully realize that the high density of leech is survived the winter cultivation.Cultural method is simple, and leech survival rate is high, and aquaculture cost is low, complete Suitable batch production leech survives the winter aquaculture model entirely;Cross-infection is avoided, During Overwintering Period disease incidence is extremely low, effectively prevents from surviving the winter Leech high-volume death condition occurs.Labour is saved, is easy to manage.Survive the winter mesh bag of being survived the winter built in casing, be advantageous to artificial Each cultivating link is controlled, cultivation is survived the winter, and leech quality is good, and overwintering survival rate is up to more than 90%, and relative weight loss rate is less than 6%;Leech Unit area quantum of output greatly improves, through statistics, compared to existing indoor leech survive the winter unit area quantum of output improve 1000/m2 More than.Solve existing indoor leech survive the winter cultivation area occupied it is big, launch leech density it is low, management trouble, the leech that survives the winter lose Rate is high again, and yield is low, it is difficult to meets that high density is survived the winter cultivation the problem of requiring.
Brief description of the drawings
Fig. 1 is the integral installation distribution structure schematic diagram of the device of surviving the winter of artificial cultivation water leech;
Fig. 2 is the structural representation of casing of surviving the winter;
Fig. 3 is the structural representation of mesh bag of surviving the winter.
In figure:1st, survive the winter casing, 2, mesh bag of surviving the winter, 3, pulley, 4, hole, 5, carriage, 6, nylon net cloth, 7, slide fastener.
Embodiment
A kind of overwintering cultivation method for propagating leech artificially;Wherein, a kind of overwintering cultivation method of artificial farming leech is Completed by the steps:
1)Need disposably to clean survive the winter casing 1 and mesh bag 2 of surviving the winter first, and at least sterilized with potassium permanganate 1 time, while take rice field Soil crosses 40 mesh sieves;
2)Mesh bag 2 of surviving the winter is inserted in each casing 1 of surviving the winter, is then mated formation in casing 1 of surviving the winter into paddy soils after treatment, Pavement thickness is 20~25cm;
3)From specification is identical, the leech of body weight error≤0.5g/ bars as leech individual of surviving the winter, 7 days before dispensing, leech is put Enter into the interior feed box set up defences and escape mesh bag, by air-conditioning by temperature control at 25 DEG C~30 DEG C, by 5~8 times of leech gross weight Fresh ox blood bowel lavage after launch and disposably fed into feed box, feeding time ensures all leech in 30~40min All feds to appetite;Feed box and casing are intended to sterilize in advance;Feeding takes out casing after terminating, then clean leech and feed box dry Only, then by fed to appetite and the leech cleaned up it is put into casing 1 of surviving the winter;
4)The proper alignment of casing 1 will be survived the winter in culturing chamber, survive the winter and people's bit manipulation space is reserved between casing 1 and casing 1 of surviving the winter; The top of each casing 1 of surviving the winter correspondingly is provided with inlet channel, and inlet channel is connected with water storage temperature-controlling system;
5)Gradually cultivation indoor temperature is adjusted to 4 DEG C~10 DEG C, keeps culturing chamber ventilated, survive the winter in casing 1 according to The density of 2500~3000/case launches leech, is controlled temperature of cultivation at 8 DEG C~12 DEG C by water storage temperature-controlling system, survives the winter 5 During individual month, temperature difference per day is no more than 2 DEG C in culturing chamber, keeps cultivation indoor environment cleaning clean, while keep breeding water body clear Clean transparent, leech initially enters the cultivation phase of surviving the winter;
6)After leech enters the cultivation phase of surviving the winter, have to check for daily once, discovery there are dead individuals to pick in time;
7)Every 7~10 days with inlet channel to 1 water drenching of casing one time of surviving the winter, ensure paddy soils humidity in 45%~55% section, Casing 1 of surviving the winter gently is loosened the soil once with diameter 2cm waddy simultaneously, but not stir leech;
8)After the cultivation phase of surviving the winter of leech terminates, statistics survives bar number, weighs the weight per case leech, calculates survival rate and relative Weight-loss ratio, survival rate to more than 90%, relative weight loss rate are less than 6%;
A kind of overwintering cultivation method for propagating leech artificially;Wherein, propagate artificially leech survive the winter device by casing 1 of surviving the winter, more Winter mesh bag 2 is formed, and casing 1 of surviving the winter is made up of thickness 0.5cm polyethylene polymers, not flexible type and fragmentation;Casing 1 of surviving the winter is in pros Body, the top for casing 1 of surviving the winter is open type case mouth, and the bottom four corners for casing 1 of surviving the winter are provided with pulley 3, are easy to carry and clean; Survive the winter it is uniform on the bottom plate of casing 1 make hole 4, during water inlet can direct draining, so as to which paddy soils are wet in the casing 1 that maintains to survive the winter Degree is between 45%-55%;Mesh bag 2 of surviving the winter is made up of carriage 5, nylon net cloth 6, slide fastener 7, and nylon net cloth 6 is enclosed mounted in carriage 5 Upper to form mesh bag 2 of surviving the winter, mesh bag 2 of surviving the winter is adapted to casing 1 of surviving the winter, and mesh bag 2 of surviving the winter is jointly mounted to survive the winter in casing 1, survives the winter The top of mesh bag 2 is provided with slide fastener 7, ensure leech survive the winter end after can not escape;The survive the winter bottom of casing 1 is mated formation and has rice field Soil;Leech, which is pierced in paddy soils, to survive the winter;
Survive the winter the 3 liftoff 10cm of pulley of casing 1, and survive the winter 1 high 60cm of casing, long 100cm, wide 100cm, the diameter 2cm of hole 4;
Survive the winter mesh bag 2 high 50cm, long 100cm, wide 100cm;The mesh of mesh bag 2 of surviving the winter is 60 mesh(Referring to Fig. 1~3).
The embodiment for propagating the overwintering cultivation method of leech artificially is as follows:
Embodiment 1:Device of surviving the winter is placed in ventilated, 4 DEG C~10 DEG C of culturing chamber, thrown by the density of 2500/case Discharge water leech, by water storage temperature-controlling system, water temperature control of surviving the winter at 8 DEG C~12 DEG C, during surviving the winter 5 months, keeps breeding environment Totally, breeding water body clean transparent;For leech survival rate after terminating of surviving the winter to 95%, relative weight loss rate is 5%.
Embodiment 2:Device of surviving the winter is placed in ventilated, 4 DEG C~10 DEG C of culturing chamber, by the close of 2800/case Degree launches leech, by water storage temperature-controlling system, water temperature control of surviving the winter at 8 DEG C~12 DEG C, during surviving the winter 5 months, keeps cultivation Environment is clean, breeding water body clean transparent;For leech survival rate after terminating of surviving the winter to 92%, relative weight loss rate is 5%.
Embodiment 3:Device of surviving the winter is placed in ventilated, 4 DEG C~10 DEG C of culturing chamber, by the close of 3000/case Degree launches leech, by water storage temperature-controlling system, water temperature control of surviving the winter at 8 DEG C~12 DEG C, during surviving the winter 5 months, keeps cultivation Environment is clean, breeding water body clean transparent;For leech survival rate after terminating of surviving the winter to 90%, relative weight loss rate is 6%.
The overwintering cultivation method for propagating leech artificially is specifically carried out by following operation:
<1>Mat formation in each casing 1 of surviving the winter into 25cm it is thick, cross the paddy soils of 40 mesh sieves, launch specification as 2.7~3.2g/ bars The leech adult 2500~3000 that health is disease-free;
<2>Cultivation indoor water storage temperature-controlling system need to ensure to survive the winter for 24 hours with water, survive the winter and clean underground water is extracted with water, need clear It is clean transparent;Water temperature is adjusted at 8 DEG C~12 DEG C by water storage temperature regulating device, temperature difference per day should be controlled at 2 DEG C;
<3>When finding that leech has haematemesis shape in During Overwintering Period, should be rinsed well in time with surviving the winter with water, and pick deformation of spitting blood Leech, carry out within 7~10 days once spraying moisturizing processing;
<4>Leech will carry out disposable feeding for 7-10 days before surviving the winter, and indoor temperature by air-conditioning device control 25 DEG C~ 30 DEG C, while leech is put into feed box and fed, it is provided with anti-escape mesh bag in feed box;Feed box first passes through new clean that in advance Go out and disinfect;It is put into after the fresh ox blood bowel lavage for weight being 5~8 times of leech gross weight in feed box, feeding time is 30 ~40 minutes;After feeding terminates, casing is taken out, leech and feed box are cleaned up together, leech, which is put into, to be already prepared to Casing of surviving the winter in, after leech is pierced in paddy soils, gradually by indoor temperature control at 4 DEG C~10 DEG C;
<5>Since surviving the winter leech, to check once daily, choose dead individuals in time;
<6>Because leech is substantially at resting state during surviving the winter, so keep indoor quiet as far as possible during whole survive the winter, Leech is not stirred during conventional maintenance management work.
In face of current leech survive the winter cultivation encounter the problem of, a kind of artificial farming leech of present invention applicant's research and design Device of surviving the winter, by surviving the winter casing 1 and mesh bag 2 of surviving the winter forms;Mat formation in casing 1 of surviving the winter paddy soils, in conjunction with water storage temperature control System, and leech biological characteristics, realize that wet soil is survived the winter high-density breeding method by 8 steps of the invention.Survive the winter cultivation Method is simple, and management is convenient, manual labor less investment, prevents cross-infection to greatest extent.During Overwintering Period disease incidence It is extremely low, prevent the generation of Large Scale Death situation, survive the winter each link and environmental factor of leech can be controlled, and ensure that leech matter of surviving the winter Amount, greatly improved leech unit area quantum of output of surviving the winter.Through statistics, survived the winter unit area quantum of output compared to existing indoor leech Improve 1000/m2More than.Leech survival rate of surviving the winter brings up to more than 90%, and existing indoor leech overwintering survival rate is up to 85% or so, and the relative weight loss rate for the leech that survives the winter is less than 6%, and the relative weight loss rate after existing indoor leech survives the winter is more than 8%. Survive the winter leech physical health, no haematemesis and deformation state occur.By it was verified that its entirely appropriate large-scale industrialized cultivation Survive the winter leech.
The example above illustrates not make any formal limitation to the substantive content of the present invention, art it is common Any letter that technical staff has been made after this specification has been read according to the technical spirit of the present invention to above embodiment Single modification deforms, and is changed or be modified to the equivalence enforcement of equivalent variations possibly also with the technology contents of the disclosure above Example, in the range of still falling within technical solution of the present invention, without departing from the spirit and scope of the invention.

Claims (1)

1. a kind of overwintering cultivation method for propagating leech artificially, it is characterised in that it is completed by the steps:
1)First, casing of surviving the winter need to disposably be cleaned(1)With mesh bag of surviving the winter(2), and at least sterilize 1 time with potassium permanganate, simultaneously Paddy soils are taken to cross 40 mesh sieves;
2)Each casing of surviving the winter(1)Inside it is inserted in mesh bag of surviving the winter(2), then in casing of surviving the winter(1)Inside mat formation into after treatment Paddy soils, pavement thickness are 20~25cm;
3)From specification is identical, the leech of body weight error≤0.5g/ bars as leech individual of surviving the winter, 7 days before dispensing, leech is put Enter into the interior feed box set up defences and escape mesh bag, by air-conditioning by temperature control at 25 DEG C~30 DEG C, by 5~8 times of leech gross weight Fresh ox blood bowel lavage after launch and disposably fed into feed box, feeding time ensures all leech in 30~40min All feds to appetite;Feed box and casing are intended to sterilize in advance;Feeding takes out casing after terminating, then clean leech and feed box dry Only, then by fed to appetite and the leech cleaned up it is put into casing of surviving the winter(1)In;
4)The proper alignment of casing 1 will be survived the winter in culturing chamber, casing of surviving the winter(1)With casing of surviving the winter(1)Between reserve people's bit manipulation Space;Each casing of surviving the winter(1)Top inlet channel is correspondingly installed, inlet channel is connected with water storage temperature-controlling system;
5)Cultivation indoor temperature is maintained at 4 DEG C~10 DEG C, ventilated, casing of surviving the winter(1)It is interior according to 2500~3000/case Density launches leech, is controlled temperature of cultivation at 8 DEG C~12 DEG C by water storage temperature-controlling system, during surviving the winter 5 months, in culturing chamber Temperature difference per day is no more than 2 DEG C, keeps cultivation indoor environment cleaning clean, while keeps breeding water body clean transparent, leech start into Enter to survive the winter the cultivation phase;
6)After leech enters the cultivation phase of surviving the winter, have to check for daily once, discovery there are dead individuals to pick in time;
7)Every 7~10 days with inlet channel to casing of surviving the winter(1)Water drenching one time, ensure paddy soil humidity in 45%~55% area Between, while with diameter 2cm waddy to casing of surviving the winter()1 gently loosens the soil once, but not stir leech;
8)After the cultivation phase of surviving the winter of leech terminates, statistics survives bar number, weighs the weight per case leech, calculates survival rate and relative Weight-loss ratio, survival rate to more than 90%, relative weight loss rate are less than 6%;
Described casing of surviving the winter(1)In square, casing of surviving the winter(1)Top be open type case mouth, casing of surviving the winter(1)Bottom Corner is provided with pulley(3), casing of surviving the winter(1)Hole is uniformly made on bottom plate(4);Survive the winter mesh bag(2)By carriage(5)、 Nylon net cloth(6), slide fastener(7)Composition, nylon net cloth(6)Enclose and be mounted in carriage(5)It is upper to form mesh bag of surviving the winter(2), mesh bag of surviving the winter (2)With casing of surviving the winter(1)Adaptation, mesh bag of surviving the winter(2)It is jointly mounted to casing of surviving the winter(1)It is interior, mesh bag of surviving the winter(2)Top installation There is slide fastener(7);Survive the winter casing(1)Bottom mat formation and have paddy soils;Survive the winter casing(1)Pulley(3)Liftoff 10cm, casing of surviving the winter (1)High 60cm, long 100cm, wide 100cm, hole(4)Diameter 2cm;Survive the winter mesh bag(2)High 50cm, long 100cm, wide 100cm;More Winter mesh bag(2)Mesh be 60 mesh.
CN201710779134.1A 2017-09-01 2017-09-01 A kind of overwintering cultivation method for propagating leech artificially Withdrawn CN107347753A (en)

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Cited By (7)

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Publication number Priority date Publication date Assignee Title
CN108184728A (en) * 2018-02-02 2018-06-22 广西三兴堂生物科技有限公司 A kind of method for cultivating hiruto
CN108782372A (en) * 2018-07-23 2018-11-13 安徽冠禅生物科技有限公司 A kind of leech cultivating escape-proof cage
CN109618994A (en) * 2018-12-25 2019-04-16 重庆多普泰制药股份有限公司 A kind of Hirudo japonica is overwintering-produce cocoon artificially controlling temperature mode
CN110692560A (en) * 2019-10-31 2020-01-17 南宁市净雪皇生物工程有限公司 Greenhouse overwintering method for artificially breeding poecilobdella manillensis
CN110771550A (en) * 2019-12-05 2020-02-11 昆明学院 Leech culture system for laboratory and culture method thereof
CN110881438A (en) * 2019-12-27 2020-03-17 安徽冠禅生物科技有限公司 Be used for heat retaining breeding device of overwintering in leech cultivation
CN111937791A (en) * 2019-05-14 2020-11-17 南京农业大学 Method for improving overwintering yield and quality of leeches

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CN203748473U (en) * 2013-12-31 2014-08-06 云南海瑞迪生物药业有限公司 Whitmania pigra overwintering and feeding net cage
CN104082249A (en) * 2014-06-30 2014-10-08 南宁市净雪皇生物工程有限公司 Poecilobdella manillensis indoor breeding device and method
CN104106496A (en) * 2013-04-22 2014-10-22 彭有款 Bag-type cultivation method for synchronously stocked leeches
CN105875508A (en) * 2014-10-24 2016-08-24 南宁市德营生物科技有限公司 New method for soilless overwintering of blood-sucking leeches

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Publication number Priority date Publication date Assignee Title
CN101766154A (en) * 2010-01-20 2010-07-07 龚元 Method for high-density green ecological culture of blood-sucking leeches
CN104106496A (en) * 2013-04-22 2014-10-22 彭有款 Bag-type cultivation method for synchronously stocked leeches
CN203748473U (en) * 2013-12-31 2014-08-06 云南海瑞迪生物药业有限公司 Whitmania pigra overwintering and feeding net cage
CN104082249A (en) * 2014-06-30 2014-10-08 南宁市净雪皇生物工程有限公司 Poecilobdella manillensis indoor breeding device and method
CN105875508A (en) * 2014-10-24 2016-08-24 南宁市德营生物科技有限公司 New method for soilless overwintering of blood-sucking leeches

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108184728A (en) * 2018-02-02 2018-06-22 广西三兴堂生物科技有限公司 A kind of method for cultivating hiruto
CN108184728B (en) * 2018-02-02 2020-12-22 广西三兴堂生物科技有限公司 Method for breeding Poecilobdella manillensis
CN108782372A (en) * 2018-07-23 2018-11-13 安徽冠禅生物科技有限公司 A kind of leech cultivating escape-proof cage
CN109618994A (en) * 2018-12-25 2019-04-16 重庆多普泰制药股份有限公司 A kind of Hirudo japonica is overwintering-produce cocoon artificially controlling temperature mode
CN111937791A (en) * 2019-05-14 2020-11-17 南京农业大学 Method for improving overwintering yield and quality of leeches
CN110692560A (en) * 2019-10-31 2020-01-17 南宁市净雪皇生物工程有限公司 Greenhouse overwintering method for artificially breeding poecilobdella manillensis
CN110771550A (en) * 2019-12-05 2020-02-11 昆明学院 Leech culture system for laboratory and culture method thereof
CN110881438A (en) * 2019-12-27 2020-03-17 安徽冠禅生物科技有限公司 Be used for heat retaining breeding device of overwintering in leech cultivation

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Inventor after: Zhang Tao

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Application publication date: 20171117