CN107318476A - A kind of Chunlan inflorescence medulla pith-cambium grafting method - Google Patents
A kind of Chunlan inflorescence medulla pith-cambium grafting method Download PDFInfo
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- CN107318476A CN107318476A CN201710589564.7A CN201710589564A CN107318476A CN 107318476 A CN107318476 A CN 107318476A CN 201710589564 A CN201710589564 A CN 201710589564A CN 107318476 A CN107318476 A CN 107318476A
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- inflorescence
- scion
- stock
- chunlan
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Soil Sciences (AREA)
- Packging For Living Organisms, Food Or Medicinal Products That Are Sensitive To Environmental Conditiond (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of Chunlan inflorescence medulla pith-cambium grafting method, this method includes（1）The storage of inflorescence；（2）The preparation of inflorescence scion；（3）The HORMONE TREATMENT of inflorescence scion；（4）The preparation of inflorescence stock；（5）Grafting；（6）Graft union processing；（7）Graft water management；（8）Graft illumination management.Orchid inflorescence, empirical tests are cultivated by the above method：The graft survival rate of Chunlan reaches 100%, and flowering rate is 100%, and the early flowering season is that the 5th 9 day after grafting, the florescence goes through long 25 35 days, the problem of solving the adjustment Chunlan florescence, with important actual application value.
Description
Technical field
The present invention relates to a kind of Chunlan inflorescence medulla pith-cambium grafting method, belong to grafting field.
Background technology
The bearing status of grafting plant is decided by scion, and stock, the uptake and accumulation of nutrient are depended on to nutrient absorption capability
Amount and distribution are decided by the joint effect of the two again.The influence that stock grows to graft is larger.Stock passes through nutrients
Matter, hormone, signal transduction material and inhereditary material etc. change regulation and control scion photosynthesis, yield, resistance, florescence, the fragrance of a flower, very
As for flower pattern and pattern.But have no that graft technology is applied in orchid cultivation at present.
Chunlan（Cymbidium goeringii(Reichb.F.) Reichb.F.) plant type is fine, delicate and pretty free from vulgarity, flower
Pigment is refined, and fragrance is quiet and beautiful, have the laudatory title of " spending middle gentleman " and " the first under heaven is fragrant ", very suitable with very high commercial value
Close industrialization development.But the time of becoming civilized concentrates on the 2-3 months, it is difficult to comply with the demand of market diversification.The Chunlan florescence lasts one
Month or so.Therefore by the grafting of Chunlan flower spike, the florescence of Chunlan is adjusted, is conducive to the industrialized development of Chunlan.
The content of the invention
In order to adjust the florescence of Chunlan, the present invention provides a kind of method that inflorescence medulla is amplexiformed, with this method grafting spring
Blue inflorescence, survival rate is high, and florescence is long.
The present invention includes following steps：
（1）The storage of inflorescence：From the unopened Chunlan inflorescence of inflorescence base portion clip, wrapped with freshness protection package, be placed on 4 DEG C of refrigerator 0-
7 days；
（2）The preparation of inflorescence scion：From the sprig of the upper cm length of clip 5 of orchid inflorescence branch, then with knife from away from terminal bud 1.5-
At 2.0 cm, scion gradually chamfers 2/5 by center.
（3）The HORMONE TREATMENT of inflorescence scion：Scion cut sides are soaked with the cotton for being saturated with hormone solution 1-3 minutes.Hormone
Solution is the mgL of BA 0.1-1+NAA 0.5 mg·L-1+ 0.2wt.% PVP。
（4）The preparation of inflorescence stock：One section thicker than scion is selected as the inflorescence of stock, side flower is removed, Ran Houyong
Knife cuts away 3/5 from the bottom up, and stock tangent plane length, width are consistent with the tangent plane of scion.
（5）Grafting：The tangent plane of scion is attached on the tangent plane of stock, is aligned up and down.
（6）Graft union processing：With sealed membrane binding grafting mouthful.Left hand holds stock, with thumb then scion lower end and
One end of plastic tape, the right hand holds the other end of plastic tape, winds from bottom to top, wants a ring to press a ring when twining, and closely ties tight, twines
After exceeding otch to upper end, then twine from top to down to after lower end, draw and withhold plastic tape end, plastic tape is not loosened.
A small bamboo rod is inserted in flowerpot, scion and stock are fixed with cotton thread.
（7）Graft water management：The white freshness protection package of set one on scion top, be at sack along stock four during bagging
Week fills out cotton, is gently tied up with cotton thread, allows cotton to be saturated with sterilized water, adds water to sooner or later daily 15 days and opens freshness protection package.
（8）Graft illumination management：Black plastic bag bagging is used, being banded in above cotton gently is broken after sheltering from heat or light 7 days
Bag.Every 2 days, strengthen by light transmittance 30% after illumination, broken bag 3 times, Hua Quanjian light.
The present invention has advantages below：
（1）The success of Chunlan grafting first.
（2）Make scion and stock with Chunlan inflorescence first.
（3）Chunlan scion is handled.With with the mgL of BA 0.1 for promoting Chunlan inflorescence Callus formation-1+ NAA
0.5 mg·L-1+ 0.2% PVP (polyvinylpyrrolidone) solution is saturated with cotton, is attached to scion tangent plane 1-3 minutes.
（4）Chunlan graft water management：The white freshness protection package of set one on scion top.Filled out at sack along stock surrounding
Cotton, is gently tied up with cotton thread, allows cotton to be saturated with sterilized water.Add water to sooner or later 15 days daily and open freshness protection package.
（5）Chunlan graft illumination management：Black plastic bag bagging is used, being banded in above cotton gently, after sheltering from heat or light 7 days
Broken bag.
Chunlan, empirical tests are cultivated by the above method：Chunlan inflorescence survival rate is to 100%, and flowering rate is 100%, first flower
Phase is that the 5-9 days after grafting, the florescence goes through long 25-35 days, the problem of solving the adjustment orchid florescence, should with important reality
With value.Same this method is readily applicable on orchid, cold orchid, the orchid inflorescence such as sword-leaved cymbidium and Chinese cymbidium.
Embodiment
The detailed process to Chunlan inflorescence engrafting method is described in detail below.
Embodiment 1
（1）The preparation of Chunlan inflorescence scion：Never open the sprig of the upper cm length of clip 5 of orchid inflorescence branch, then with knife from away from
At the cm of terminal bud 2.0, scion gradually chamfers 2/5 by center.
（2）The HORMONE TREATMENT of Chunlan inflorescence scion：With being saturated with the mgL of BA 0.1-1+NAA 0.5 mg·L-1+ 0.2%
The cotton immersion scion cut sides of PVP (polyvinylpyrrolidone) solution 1 minute.
（3）The preparation of Chunlan inflorescence stock：One section thicker than scion is selected as the inflorescence of stock, side flower is removed, so
Cut away 3/5 from the bottom up with knife afterwards, stock tangent plane length, width are consistent with the tangent plane of scion.
（4）Grafting：The tangent plane of scion is attached on the tangent plane of stock, is aligned up and down.
（5）Graft union processing：With sealed membrane binding grafting mouthful.Left hand holds stock, with thumb then scion lower end and
One end of plastic tape, the right hand holds the other end of plastic tape, winds from bottom to top, wants a ring to press a ring when twining, and suitably ties tight, twines
After exceeding otch to upper end, then twine from top to down to after lower end, draw and withhold plastic tape end, plastic tape is not loosened.
A small bamboo rod is inserted in flowerpot, scion and stock are fixed with cotton thread.
（6）Graft water management：The white freshness protection package of set one on scion top, be at sack along stock four during bagging
Week fills out cotton, is gently tied up with cotton thread, allows cotton to be saturated with sterilized water, adds water to sooner or later daily 15 days and opens freshness protection package.
（7）Graft illumination management：Black plastic bag bagging is used, being banded in above cotton gently is broken after sheltering from heat or light 7 days
Bag.Every 2 days, strengthen by light transmittance 30% after illumination, broken bag 3 times, Hua Quanjian light.
Orchid inflorescence survival rate is to 100%, and flowering rate is 100%, and the early flowering season is that the florescence goes through length the 5th day after grafting
35 days.
Embodiment 2
（1）The storage of Chunlan inflorescence：From the unopened Chunlan inflorescence of inflorescence base portion clip, wrapped with freshness protection package, be placed on 4 DEG C of ice
Case 3 days；
（2）The preparation of Chunlan inflorescence scion：From the sprig of the upper cm length of clip 5 of orchid inflorescence branch, then with knife from away from terminal bud
At 1.5cm, scion gradually chamfers 2/5 by center.
（3）The HORMONE TREATMENT of Chunlan inflorescence scion：With being saturated with by the mgL of BA 0.1-1+NAA 0.5 mg·L-1+
The cotton immersion scion cut sides of 0.2% PVP (polyvinylpyrrolidone) solution 2 minutes.
（4）The preparation of inflorescence stock：One section thicker than scion is selected as the inflorescence of stock, side flower is removed, Ran Houyong
Knife cuts away 3/5 from the bottom up, and stock tangent plane length, width are consistent with the tangent plane of scion.
（5）Grafting：The tangent plane of scion is attached on the tangent plane of stock, is aligned up and down.
（6）Graft union processing：With sealed membrane binding grafting mouthful.Left hand holds stock, with thumb then scion lower end and
One end of plastic tape, the right hand holds the other end of plastic tape, winds from bottom to top, wants a ring to press a ring when twining, and suitably ties tight, twines
After exceeding otch to upper end, then twine from top to down to after lower end, draw and withhold plastic tape end, plastic tape is not loosened.
A small bamboo rod is inserted in flowerpot, scion and stock are fixed with cotton thread.
（7）Graft water management：The white freshness protection package of set one on scion top, be at sack along stock four during bagging
Week fills out cotton, is gently tied up with cotton thread, allows cotton to be saturated with sterilized water, adds water to sooner or later daily 15 days and opens freshness protection package.
（8）Graft illumination management：Black plastic bag bagging is used, being banded in above cotton gently is broken after sheltering from heat or light 7 days
Bag.Every 2 days, strengthen by light transmittance 30% after illumination, broken bag 3 times, Hua Quanjian light.
Chunlan inflorescence survival rate is to 100%, and flowering rate is 100%, and the early flowering season is that the florescence goes through length the 7th day after grafting
35 days.
Embodiment 3
（1）The storage of Chunlan inflorescence：From the unopened Chunlan inflorescence of inflorescence base portion clip, wrapped with freshness protection package, be placed on 4 DEG C of ice
Case 7 days；
（2）The preparation of inflorescence scion：From the sprig of the upper cm length of clip 5 of orchid inflorescence branch, then with knife from away from the cm of terminal bud 2.0
Place, scion gradually chamfers 2/5 by center.
（3）The HORMONE TREATMENT of inflorescence scion：With being saturated with by the mgL of BA 0.1-1+NAA 0.5 mg·L-1+ 0.2%
The cotton immersion scion cut sides of PVP (polyvinylpyrrolidone) solution 3 minutes.
（4）The preparation of inflorescence stock：One section thicker than scion is selected as the inflorescence of stock, side flower is removed, Ran Houyong
Knife cuts away 3/5 or so from the bottom up, and stock tangent plane length, width are consistent with the tangent plane of scion.
（5）Grafting：The tangent plane of scion is attached on the tangent plane of stock, is aligned up and down.
（6）Graft union processing：With sealed membrane binding grafting mouthful.Left hand holds stock, with thumb then scion lower end and
One end of plastic tape, the right hand holds the other end of plastic tape, winds from bottom to top, wants a ring to press a ring when twining, and suitably ties tight, twines
After exceeding otch to upper end, then twine from top to down to after lower end, draw and withhold plastic tape end, plastic tape is not loosened.
A small bamboo rod is inserted in flowerpot, scion and stock are fixed with cotton thread.
（7）Graft water management：The white freshness protection package of set one on scion top, be at sack along stock four during bagging
Week fills out cotton, is gently tied up with cotton thread, allows cotton to be saturated with sterilized water, adds water to sooner or later daily 15 days and opens freshness protection package.
（8）Graft illumination management：Black plastic bag bagging is used, being banded in above cotton gently is broken after sheltering from heat or light 7 days
Bag.Every 2 days, strengthen by light transmittance 30% after illumination, broken bag 3 times, Hua Quanjian light.
Chunlan inflorescence survival rate is to 100%, and flowering rate is 100%, and the early flowering season is that the florescence goes through length the 9th day after grafting
25 days.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (2)
1. a kind of Chunlan inflorescence medulla pith-cambium grafting method, it is characterised in that：Comprise the steps of：
The storage of inflorescence：From the unopened Chunlan inflorescence of inflorescence base portion clip, wrapped with freshness protection package, be placed on 4 DEG C of refrigerator 0-7
My god；
The preparation of inflorescence scion：From the sprig of the upper cm length of clip 5 of orchid inflorescence branch, then with knife from away from terminal bud 1.5-2.0 cm
Place, scion gradually chamfers 2/5 by center；
（3）The HORMONE TREATMENT of inflorescence scion：Scion cut sides are soaked with the cotton for being saturated with hormone solution 1-3 minutes；
（4）The preparation of inflorescence stock：One section thicker than scion is selected as the inflorescence of stock, side flower is removed, knife is then used
3/5 is cut away from the bottom up, and stock tangent plane length, width are consistent with the tangent plane of scion；
（5）Grafting：The tangent plane of scion is attached on the tangent plane of stock, is aligned up and down；
（6）Graft union processing：With sealed membrane binding grafting mouthful；Left hand holds stock, with thumb then scion lower end and plastics
One end of band, the right hand holds the other end of plastic tape, winds from bottom to top, wants a ring to press a ring when twining, and closely ties tight, twines to upper
End exceedes after otch, then twines from top to down to after lower end, draws and withholds plastic tape end, plastic tape is not loosened；In flower
A small bamboo rod is inserted in basin, scion and stock are fixed with cotton thread；
（7）Graft water management：The white freshness protection package of set one on scion top, during bagging, will be filled out along stock surrounding at sack
Upper cotton, is gently tied up with cotton thread, allows cotton to be saturated with sterilized water, is added water to sooner or later daily 15 days and is opened freshness protection package；
（8）Graft illumination management：Black plastic bag bagging is used, being banded in above cotton gently, broken bag after sheltering from heat or light 7 days；Often
Every 2 days, strengthen by light transmittance 30% after illumination, broken bag 3 times, Hua Quanjian light.
2. a kind of Chunlan inflorescence medulla pith-cambium grafting method according to claim 1, it is characterised in that：Step（3）Middle hormone solution
For the mgL of BA 0.1-1+NAA 0.5 mg·L-1+ 0.2wt.% PVP。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710589564.7A CN107318476B (en) | 2017-07-19 | 2017-07-19 | Method for grafting cymbidium goeringii inflorescence medulla |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710589564.7A CN107318476B (en) | 2017-07-19 | 2017-07-19 | Method for grafting cymbidium goeringii inflorescence medulla |
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| Publication Number | Publication Date |
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| CN107318476A true CN107318476A (en) | 2017-11-07 |
| CN107318476B CN107318476B (en) | 2020-02-14 |
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| CN201710589564.7A Active CN107318476B (en) | 2017-07-19 | 2017-07-19 | Method for grafting cymbidium goeringii inflorescence medulla |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108040733A (en) * | 2018-01-04 | 2018-05-18 | 湖北瑞晟生物有限责任公司 | A kind of technology for the grafting success rate for improving Camellia nitidissima |
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| CN1219344A (en) * | 1997-12-09 | 1999-06-16 | 李海根 | Chrysanthemum tree and its culture method |
| CN103688766A (en) * | 2013-12-12 | 2014-04-02 | 南通安立可农产品科技有限公司 | Cultivation method of golden chrysanthemum |
| CN106386193A (en) * | 2016-12-12 | 2017-02-15 | 商丘师范学院 | Fast grafting method of chrysanthemums |
| CN106561288A (en) * | 2015-10-08 | 2017-04-19 | 戚友亦 | Method for grafting of chrysanthemum with Artemisia lavandulaefolia |
| CN106561272A (en) * | 2015-10-08 | 2017-04-19 | 黄冠洲 | Method for grafting chrysanthemum to Artemisia abaensis |
-
2017
- 2017-07-19 CN CN201710589564.7A patent/CN107318476B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1219344A (en) * | 1997-12-09 | 1999-06-16 | 李海根 | Chrysanthemum tree and its culture method |
| CN103688766A (en) * | 2013-12-12 | 2014-04-02 | 南通安立可农产品科技有限公司 | Cultivation method of golden chrysanthemum |
| CN106561288A (en) * | 2015-10-08 | 2017-04-19 | 戚友亦 | Method for grafting of chrysanthemum with Artemisia lavandulaefolia |
| CN106561272A (en) * | 2015-10-08 | 2017-04-19 | 黄冠洲 | Method for grafting chrysanthemum to Artemisia abaensis |
| CN106386193A (en) * | 2016-12-12 | 2017-02-15 | 商丘师范学院 | Fast grafting method of chrysanthemums |
Non-Patent Citations (1)
| Title |
|---|
| 魏军; 王继涛: "菊花二乔斗艳等几种艺术造型的靠接方法", 《吉林蔬菜》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108040733A (en) * | 2018-01-04 | 2018-05-18 | 湖北瑞晟生物有限责任公司 | A kind of technology for the grafting success rate for improving Camellia nitidissima |
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| CN107318476B (en) | 2020-02-14 |
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