CN107307231A - It is a kind of to prevent Chinese herbal feed additive of golden trout stigmatosis and application thereof - Google Patents

It is a kind of to prevent Chinese herbal feed additive of golden trout stigmatosis and application thereof Download PDF

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Publication number
CN107307231A
CN107307231A CN201710595420.2A CN201710595420A CN107307231A CN 107307231 A CN107307231 A CN 107307231A CN 201710595420 A CN201710595420 A CN 201710595420A CN 107307231 A CN107307231 A CN 107307231A
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parts
stigmatosis
chinese herbal
golden trout
feed additive
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刘伟
任倩妍
王日昕
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Tonghua Normal University
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Tonghua Normal University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • A01K61/13Prevention or treatment of fish diseases
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/10Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

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  • Life Sciences & Earth Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Environmental Sciences (AREA)
  • Physiology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Insects & Arthropods (AREA)
  • Health & Medical Sciences (AREA)
  • Birds (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Fodder In General (AREA)

Abstract

The invention discloses a kind of Chinese herbal feed additive of prevention golden trout stigmatosis and application thereof, the composition and its parts by weight of additive are:15 20 parts of purslane, 22 25 parts of honeysuckle, 18 22 parts of rheum officinale, 11 15 parts of tripterygium wilfordii, 18 22 parts of cordate houttuynia, 10 15 parts of the Radix Astragali, 25 parts of radix glycyrrhizae, 47 parts of rhizoma atractylodis, 23 parts of barrenwort, 47 parts of garden burnet.Have the beneficial effect that:The raw material of the feed addictive of the present invention is Chinese herbal medicine, can be degradable, it is pollution-free to fishpond, effect with clearing heat and detoxicating sterilization antibacterial, infringement that can be with microorganism outside defense cell and its toxin to body can prevent the disease hair of golden trout stigmatosis, stigmatosis can be treated, therapeutic effect is good, and can improve the immunity of golden trout, and promotes the generation of stigmatosis antibody.

Description

It is a kind of to prevent Chinese herbal feed additive of golden trout stigmatosis and application thereof
Technical field
The present invention relates to aquaculture feeds additive processing technique field, more particularly to a kind of prevention golden trout stigmatosis Chinese herbal feed additive and application thereof.
Background technology
Golden trout, salmon shape mesh salmonidae Pacific Ocean salmon category belongs to cold water fishes, and meat flavour is delicious, nutritious, and the few meat of thorn is more, food It is high with value, it is to integrate view and admire, swim fishing, the improved seeds of cuisines.A kind of fish occur in golden trout breeding process Common disease --- stigmatosis.Stigmatosis is that cause of disease scale in the body surface of dorsal fin has subcircular erythema, focus comes off, and is eventually formed Ulcer even exposes bone or internal organ.Lift golden trout immunity therefore, it is necessary to provide a kind of can improve, prevent stigmatosis Feed addictive, so as to avoid the economic loss caused in golden trout breeding process due to stigmatosis.Chinese herbal medicine conduct A kind of natural Substitutes For Antibiotic increasingly attracts attention, and fish production practice also demonstrates that Chinese herbal medicine is used as mixed feeding agent It is simultaneous or feed addictive can fully ensure that the health of human body while aquatic livestock production performance and feed efficiency is improved again There is the double action of the property of medicine and trophism, be that traditional chemical synthesis feed addictive is incomparable, be particularly suitable for use in current The need for the intensive of culture fishery, large-scale production.Therefore, with the additive of Chinese herbal medicine substitute antibiotics will increasingly by To the concern of people, its application prospect also can be more extensive.But the research level of current Chinese herbal feed additive is relatively low, makes Technique simple coarse, the technological element of a product is relatively low, and quality is not sufficiently stable, and such problem seriously governs Chinese medicine fodder addition Popularization and application.At present, do not disclose also both at home and abroad any on the Chinese herbal medicine for improving golden trout immunity, preventing stigmatosis Feed addictive and its relevant report of application.
Prior art such as Authorization Notice No. is the B of CN 103892051 Chinese invention patent, discloses a kind of preventing and treating mullet The Chinese herbal feed additive of hemorrhage, raw material includes golden cypress, rheum officinale, radix glycyrrhizae, chrysanthemum, selfheal, stringy stonecrop, preparation method It is:Said components are put into mixer, after being sufficiently mixed, is crushed in micronizer, the material after crushing is sieved Net, is made the Chinese herb feed additive of preventing and treating mullet hemorrhage.The morbidity of mullet hemorrhage can be reduced after this feed addictive Rate, the immune and oxidation resistance for improving mullet, enhancing resistance against diseases, but the Ultramicro-powder that the feed addictive is used in preparing Broken method is unreasonable, and sporoderm-broken rate is low, causes Chinese herbal medicine utilization rate low.
The content of the invention
It is extensive, green, environmentally friendly it is an object of the invention to provide a kind of raw material sources, the bacillary gill of golden trout can be prevented Inflammation, improves absorbing for golden trout, effect of drugs significantly prevents Chinese herbal feed additive of golden trout stigmatosis and application thereof.
The present invention is directed to the problem of being mentioned in above-mentioned technology, and the technical scheme taken is:A kind of prevention golden trout stigmatosis Chinese herbal feed additive, composition and its parts by weight are:15-20 parts of purslane, 22-25 parts of honeysuckle, 18-22 parts of rheum officinale, thunder Public rattan 11-15 parts, 18-22 parts of cordate houttuynia, 10-15 parts of the Radix Astragali, 2-5 parts of radix glycyrrhizae, 4-7 parts of rhizoma atractylodis, 2-3 parts of barrenwort, garden burnet 4-7 Part.The raw material of above-mentioned additive is Chinese herbal medicine, can be degradable, pollution-free to fishpond, with clearing heat and detoxicating, sterilization antibacterial Effect, infringement that can be with microorganism outside defense cell and its toxin to body can prevent the disease hair of golden trout stigmatosis, can treat Stigmatosis, therapeutic effect is good, and can improve the immunity of golden trout, and promotes the generation of stigmatosis antibody.
A kind of preparation method of the Chinese herbal feed additive of prevention golden trout stigmatosis, specifically includes following steps:
Herbal raw material is distinguished into size-reduced machine to crush, crosses 100-120 mesh sieves and obtains coarse granule, thick of each raw material is measured by formula Grain, is well mixed, and adds mixture into water and active peptide, and the addition of water is 2-3 times of compound, and the addition of active peptide is The 0.003-0.004% of compound weight, is put into micronizer and crushes 30-40min, and it is 15-25 to control ultramicro grinding temperature DEG C, spray drying produces Chinese herbal feed additive after crushing terminates, and the amino acid sequence of active peptides is HSHACKLCVCVNCFCCGAAKCYLCRVLCSK, the active peptides may act on the coarse grained cell membrane of Chinese herbal medicine, on film The ion channel of cross-film is formed, the integrality of cell membrane is destroyed, comes out intracellular active drug composition quick release, greatly It is big to improve the sporoderm-broken rate of ultramicro grinding, and the activity of active ingredient is not influenceed;Ultramicro grinding can destroy Chinese herbal medicine in the step Cell membrane, cell-wall breaking ratio is not less than 95%, intracellular effective drug ingedient is fully exposed, improves the life of medicine Thing utilization rate, the consumption for reducing Chinese medicine, medium-height grass the effective elements of the medicine can effectively discharge rapidly in golden trout enteron aisle, golden trout stomach Enteron aisle can be absorbed effectively, improve drug effect, and improve the uniformity of particle to a certain extent, raise Chinese herbal medicine Feed additives are easier to be dispersed in feed.
A kind of purposes of the Chinese herbal feed additive of prevention golden trout stigmatosis:Chinese herbal feed addition is carried out in feed Feed, addition is the 0.05-0.09% of golden trout feed relative, on the one hand the feed has the work of clearing heat and detoxicating sterilization antibacterial With can eliminate into the pathogen in golden trout body and parasite, reach the purpose of prevention golden trout stigmatosis, on the other hand add The addition of agent can be effectively facilitated digestibility and utilization of the feed nutrition material in golden trout enteron aisle, improve the immunocompetence of golden trout itself And resistance against diseases, the cultivation survival rate of golden trout is improved, increases the income of raiser.
Compared with prior art, the advantage of the invention is that:The raw material of additive is Chinese herbal medicine, can be degradable, right Fishpond is pollution-free, the effect with clearing heat and detoxicating sterilization antibacterial, body can be invaded with microorganism outside defense cell and its toxin Evil, can prevent the disease hair of golden trout stigmatosis, can treat stigmatosis, therapeutic effect is good, and can improve the immunity of golden trout, and promotees Enter the generation of stigmatosis antibody;The mode that the present invention is combined using active peptides and ultramicro grinding, can destroy Chinese herbal medicine thin Cell wall and cell membrane, make intracellular effective drug ingedient fully be exposed, improve medicine bioavailability, reduce in The consumption of medicine, while improving performance and the value of Chinese herbal medicine drug effect;Chinese herbal feed additive of the present invention is added in feed, makes Obtaining feed has the effect of prevention golden trout stigmatosis, and can be effectively facilitated digestion profit of the feed nutrition material in golden trout enteron aisle With the immunocompetence and resistance against diseases of raising golden trout itself improve the cultivation survival rate of golden trout.
Specific embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
A kind of Chinese herbal feed additive of prevention golden trout stigmatosis, composition and its parts by weight are:18 parts of purslane, honeysuckle 23 Part, 20 parts of rheum officinale, 13 parts of tripterygium wilfordii, 20 parts of cordate houttuynia, 13 parts of the Radix Astragali, 3 parts of radix glycyrrhizae, 6 parts of rhizoma atractylodis, 2.5 parts of barrenwort, garden burnet 6 Part.
A kind of preparation method of the Chinese herbal feed additive of prevention golden trout stigmatosis, is concretely comprised the following steps:
Herbal raw material is distinguished into size-reduced machine to crush, crosses 120 mesh sieves and obtains coarse granule, each raw material coarse granule is measured by formula, mix Close uniform, add mixture into water and active peptide, the addition of water is 2.5 times of compound, the addition of active peptide is mixing Expect the 0.0035% of weight, be put into micronizer and crush 35min, it is 15-25 DEG C to control ultramicro grinding temperature, and crushing terminates Spray drying produces Chinese herbal feed additive afterwards, and the amino acid sequence of active peptides is HSHACKLCVCVNCFCCGAAKCYLCRVLCSK, the active peptides may act on the coarse grained cell membrane of Chinese herbal medicine, on film The ion channel of cross-film is formed, the integrality of cell membrane is destroyed, comes out intracellular active drug composition quick release, greatly It is big to improve the sporoderm-broken rate of ultramicro grinding, and the activity of active ingredient is not influenceed;Ultramicro grinding can destroy Chinese herbal medicine in the step Cell membrane, cell-wall breaking ratio is not less than 95%, intracellular effective drug ingedient is fully exposed, improves the life of medicine Thing utilization rate, the consumption for reducing Chinese medicine, medium-height grass the effective elements of the medicine can effectively discharge rapidly in golden trout enteron aisle, golden trout stomach Enteron aisle can be absorbed effectively, improve drug effect, and improve the uniformity of particle to a certain extent, raise Chinese herbal medicine Feed additives are easier to be dispersed in feed.
A kind of purposes of the Chinese herbal feed additive of prevention golden trout stigmatosis:Chinese herbal feed addition is carried out in feed Feed, addition is the 0.07% of golden trout feed relative, on the one hand the feed has the effect of clearing heat and detoxicating sterilization antibacterial, can Eliminate enter golden trout body in pathogen and parasite, reach prevention golden trout stigmatosis purpose, another aspect additive plus Enter the digestibility and utilization that can be effectively facilitated feed nutrition material in golden trout enteron aisle, improve the immunocompetence of golden trout itself and disease-resistant Ability, improves the cultivation survival rate of golden trout, increases the income of raiser.
Embodiment 2:
A kind of Chinese herbal feed additive of prevention golden trout stigmatosis, composition and its parts by weight are:16 parts of purslane, honeysuckle 25 Part, 18 parts of rheum officinale, 15 parts of tripterygium wilfordii, 18 parts of cordate houttuynia, 15 parts of the Radix Astragali, 5 parts of radix glycyrrhizae, 4 parts of rhizoma atractylodis, 2.3 parts of barrenwort, garden burnet 7 Part.
A kind of preparation method of the Chinese herbal feed additive of prevention golden trout stigmatosis, is concretely comprised the following steps:
Herbal raw material is distinguished into size-reduced machine to crush, crosses 100 mesh sieves and obtains coarse granule, each raw material coarse granule is measured by formula, mix Close uniform, add mixture into water and active peptide, the addition of water is 2 times of compound, the addition of active peptide is compound The 0.004% of weight, is put into micronizer and crushes 30min, and it is 15-25 DEG C to control ultramicro grinding temperature, and crushing is sprayed after terminating Mist is drying to obtain Chinese herbal feed additive, and the amino acid sequence of active peptides is HSHACKLCVCVNCFCCGAAKCYLCRVLCSK, the active peptides may act on the coarse grained cell membrane of Chinese herbal medicine, on film The ion channel of cross-film is formed, the integrality of cell membrane is destroyed, comes out intracellular active drug composition quick release, greatly It is big to improve the sporoderm-broken rate of ultramicro grinding, and the activity of active ingredient is not influenceed;Ultramicro grinding can destroy Chinese herbal medicine in the step Cell membrane, cell-wall breaking ratio is not less than 95%, intracellular effective drug ingedient is fully exposed, improves the life of medicine Thing utilization rate, the consumption for reducing Chinese medicine, medium-height grass the effective elements of the medicine can effectively discharge rapidly in golden trout enteron aisle, golden trout stomach Enteron aisle can be absorbed effectively, improve drug effect, and improve the uniformity of particle to a certain extent, raise Chinese herbal medicine Feed additives are easier to be dispersed in feed.
A kind of purposes of the Chinese herbal feed additive of prevention golden trout stigmatosis:Chinese herbal feed addition is carried out in feed Feed, addition is the 0.05% of golden trout feed relative, on the one hand the feed has the effect of clearing heat and detoxicating sterilization antibacterial, can Eliminate enter golden trout body in pathogen and parasite, reach prevention golden trout stigmatosis purpose, another aspect additive plus Enter the digestibility and utilization that can be effectively facilitated feed nutrition material in golden trout enteron aisle, improve the immunocompetence of golden trout itself and disease-resistant Ability, improves the cultivation survival rate of golden trout, increases the income of raiser.
Embodiment 3:
A kind of Chinese herbal feed additive of prevention golden trout stigmatosis, composition and its parts by weight are:20 parts of purslane, honeysuckle 22 Part, 21 parts of rheum officinale, 11 parts of tripterygium wilfordii, 20 parts of cordate houttuynia, 11 parts of the Radix Astragali, 5 parts of radix glycyrrhizae, 4 parts of rhizoma atractylodis, 3 parts of barrenwort, 4 parts of garden burnet.
A kind of preparation method of the Chinese herbal feed additive of prevention golden trout stigmatosis, specifically includes following steps:
Herbal raw material is distinguished into size-reduced machine to crush, crosses 120 mesh sieves and obtains coarse granule, each raw material coarse granule is measured by formula, mix Close uniform, add mixture into water and active peptide, the addition of water is 3 times of compound, the addition of active peptide is compound The 0.003% of weight, is put into micronizer and crushes 40min, and it is 15-25 DEG C to control ultramicro grinding temperature, and crushing is sprayed after terminating Mist is drying to obtain Chinese herbal feed additive, and the amino acid sequence of active peptides is HSHACKLCVCVNCFCCGAAKCYLCRVLCSK。
A kind of purposes of the Chinese herbal feed additive of prevention golden trout stigmatosis:Chinese herbal feed addition is carried out in feed Feed, addition is the 0.09% of golden trout feed relative.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention, Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Tonghua Teachers College
<120>It is a kind of to prevent Chinese herbal feed additive of golden trout stigmatosis and application thereof
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 30
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Lys Leu Cys Val Cys Val Asn Cys Phe Cys Cys
1 5 10 15
Gly Ala Ala Lys Cys Tyr Leu Cys Arg Val Leu Cys Ser Lys
20 25 30

Claims (9)

1. a kind of Chinese herbal feed additive of prevention golden trout stigmatosis, it is characterised in that:The composition of described additive and its Parts by weight are:15-20 parts of purslane, 22-25 parts of honeysuckle, 18-22 parts of rheum officinale, 11-15 parts of tripterygium wilfordii, 18-22 parts of cordate houttuynia, 10-15 parts of the Radix Astragali, 2-5 parts of radix glycyrrhizae, 4-7 parts of rhizoma atractylodis, 2-3 parts of barrenwort, 4-7 parts of garden burnet.
2. a kind of Chinese herbal feed additive of prevention golden trout stigmatosis according to claim 1, it is characterised in that:It is described The preparation method of additive concretely comprise the following steps:Herbal raw material is distinguished into size-reduced machine to crush, sieve to obtain coarse granule, by with Side measures each raw material coarse granule, is well mixed, adds mixture into water and active peptide, is put into micronizer and crushes, and crushes Spray drying produces Chinese herbal feed additive after end.
3. a kind of preparation method of the Chinese herbal feed additive of prevention golden trout stigmatosis according to claim 2, it is special Levy and be:Described coarse granule was 100-120 mesh sieves.
4. a kind of preparation method of the Chinese herbal feed additive of prevention golden trout stigmatosis according to claim 2, it is special Levy and be:The amino acid sequence of described active peptides is HSHACKLCVCVNCFCCGAAKCYLCRVLCSK.
5. a kind of preparation method of the Chinese herbal feed additive of prevention golden trout stigmatosis according to claim 2, it is special Levy and be:The addition of described active peptides is the 0.003-0.004% of compound weight.
6. a kind of preparation method of the Chinese herbal feed additive of prevention golden trout stigmatosis according to claim 2, it is special Levy and be:The addition of described water is 2-3 times of compound.
7. a kind of preparation method of the Chinese herbal feed additive of prevention golden trout stigmatosis according to claim 2, it is special Levy and be:Described ultramicro grinding 30-40min.
8. a kind of preparation method of the Chinese herbal feed additive of prevention golden trout stigmatosis according to claim 2, it is special Levy and be:Described ultramicro grinding temperature is 15-25 DEG C.
9. a kind of purposes of the Chinese herbal feed additive of prevention golden trout stigmatosis, it is characterised in that:Described Chinese herbal feed Addition is fed in feed, and addition is the 0.05-0.09% of golden trout feed relative.
CN201710595420.2A 2017-07-20 2017-07-20 It is a kind of to prevent Chinese herbal feed additive of golden trout stigmatosis and application thereof Pending CN107307231A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108782998A (en) * 2018-06-04 2018-11-13 广州市联鲲生物科技有限公司 A kind of Ba Shayu premixes and its preparation method and application

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101152391A (en) * 2007-09-30 2008-04-02 北京凯瑞创新医药科技有限公司 Pharmaceutical formulations for treating traumatic injury, preparing method and quality control method thereof
CN104872392A (en) * 2015-05-27 2015-09-02 石东秀 Feed for treating rotten-skin disease of cyclemys trifasciata and preparation method of feed
CN105249003A (en) * 2015-10-29 2016-01-20 青岛海之源智能技术有限公司 Feed additive for preventing stigmatosis of Siamese fighting fish and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101152391A (en) * 2007-09-30 2008-04-02 北京凯瑞创新医药科技有限公司 Pharmaceutical formulations for treating traumatic injury, preparing method and quality control method thereof
CN104872392A (en) * 2015-05-27 2015-09-02 石东秀 Feed for treating rotten-skin disease of cyclemys trifasciata and preparation method of feed
CN105249003A (en) * 2015-10-29 2016-01-20 青岛海之源智能技术有限公司 Feed additive for preventing stigmatosis of Siamese fighting fish and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108782998A (en) * 2018-06-04 2018-11-13 广州市联鲲生物科技有限公司 A kind of Ba Shayu premixes and its preparation method and application
CN108782998B (en) * 2018-06-04 2021-11-26 广州市联鲲生物科技有限公司 Premix for basha fish and preparation method and application thereof

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