CN105249003A - Feed additive for preventing stigmatosis of Siamese fighting fish and preparation method thereof - Google Patents
Feed additive for preventing stigmatosis of Siamese fighting fish and preparation method thereof Download PDFInfo
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- CN105249003A CN105249003A CN201510724226.0A CN201510724226A CN105249003A CN 105249003 A CN105249003 A CN 105249003A CN 201510724226 A CN201510724226 A CN 201510724226A CN 105249003 A CN105249003 A CN 105249003A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention relates to a feed additive for preventing stigmatosis of Siamese fighting fish, and further relates to a preparation method of the feed additive for preventing the stigmatosis of the Siamese fighting fish. The feed additive for preventing the stigmatosis of the Siamese fighting fish is prepared from the following components in parts by weight: 27-35 parts of tea water, 20-26 parts of blackend swallowwort root, 19-25 parts of parslane herb, 19-25 parts of mexican tea herb, 19-25 parts of chinese honeylocust fruit, 17-23 parts of common bluebeard herb,15-23 parts of heartleaf houttuynia herb, 14-22 parts of stone-like omphalia, 14-20 parts of betel nut, 12-20 parts of centipede, 13-19 parts of seed of chinese milkvetch, 12-19 parts of india madder root, 9-17 parts of scorpion, 12-20 parts of fish oil, 9-15 parts of honey, 11-19 parts of dried earthworm powder, 8-15 parts of shell powder, 7-15 parts of dried yeast powder and the like. The feed additive for preventing the stigmatosis of the Siamese fighting fish can effectively prevent the stigmatosis of the Siamese fighting fish from occurring.
Description
Technical field
The invention belongs to technical field of aquaculture, particularly relate to a kind of feed addictive preventing betta splendens stigmatosis; The invention still further relates to a kind of preparation method of feed addictive of above-mentioned prevention betta splendens stigmatosis.
Background technology
Along with the growth in the living standard of people, material life is enriched greatly, and the requirement that people live to amusement and recreation is more and more obvious, and staying at home or supporting pet fish beautiful in colour a bit in garden is best selection, takes up room hardly because raise pet fish; Economic cost can be determined according to people; Condition is relatively simple, has water.Cultivation of ornamental fish not only can be cultivated the moral character and nourished the nature, and cultivates one's taste, and the anion discharged in water and steam also can filter and wet air, build good living environment to people.
Betta splendens, originates in Thailand, and the five colours that are otherwise known as are fought fish.The same with other bucket fish products kind, the adaptive capacity of environment is very strong, and the gorgeous exaggeration of profile belongs to than one of pet fish being easier to raise.Due to the aggressive of betta splendens milter, a long time ago, a lot of places is just had it to be considered as a kind of instrument of amusement.Betta splendens is with bellicose well-known, and two male phases are met and must be carried out field decisive struggle, magnify cheek lid and fin mutually, impact provocation mutually, then bait mutually with mouth with health during bucket.Therefore, in raising, adult milters more than 2 tails can not be put in a suitable place to breed 1 cylinder.
Stigmatosis is also known as putrid skin disease, and its pathogen is the point-like subspecies of point-like Aerononas punctata, belongs to Gram-negative bacteria.It is that disease fish starts inflammation at anal both sides or caudal peduncle position (only a few is anterior at health), skin, muscle usually that bucket fish suffers from the cardinal symptom after stigmatosis, there is erythema, sometimes like warts shape, along with PD, the scale at this position comes off, and muscle rots gradually, forms rubescent, the rounded or oval focus of margin hyperemia, seem to stamp a Red Sigil, therefore be referred to as stigmatosis.Sick fish gynecological ailments, swims blunt; When falling ill serious, death can be there is successively.When water quality is unclean or fish body damaged time, often can infect that this is sick.There is no obvious epidemic season.At present, stigmatosis for bucket fish there is no special drug therapy, the means of usual employing within 30 minutes, carry out disinfection to sick fish with 2 grams of/cubic metre of water body furazolidone dipping fish bodies, but its result for the treatment of is limited, toxicity is comparatively large, easily pollutes environment, and operation is comparatively loaded down with trivial details, operating process very easily causes the scale of bucket fish to come off, while sterilization is prevented or cure a disease, also result in larger damage to bucket fish.Therefore, that prevents stigmatosis has important production meaning, develops a kind of environmental protection, can effectively prevent betta splendens to print pathogenetic feed or feed addictive, have important economic implications and market prospects.
Summary of the invention
An object of the present invention is to provide a kind of feed addictive preventing betta splendens stigmatosis, its raw material components is the natural material of environmental protection, there is good restraining and sterilizing bacteria effect to the pathogen point-like Aerononas punctata of betta splendens stigmatosis, effectively can reduce the incidence of disease of betta splendens stigmatosis, the immunity improving betta splendens and survival rate.
In order to solve the problems of the technologies described above, the present invention by the following technical solutions, a kind of feed addictive preventing betta splendens stigmatosis, be made up of each component of following masses number: tea 27-35 part, radix cynanchi atrati 20-26 part, purslane 19-25 part, chenopodium ambrosiodies 19-25 part, Chinese honey locust 19-25 part, moutan bark 17-23 part, oriental wormwood grass 17-23 part, cordate houttuynia 15-23 part, selfheal 16-22 part, Chinese gall 15-21 part, stone-like omphalia 14-22 part, betel nut 14-20 part, centipede 12-20 part, Chinese milk vetch 13-19 part, shepherd's purse 13-18 part, madder 12-19 part, English white 11-19 part, ginger 10-19 part, garlic 10-17 part, scorpion 9-17 part, Asiatic plantain 9-17 part, bamboo salt 8-16 part, light-coloured vinegar 8-15 part, fish oil 12-20 part, honey 9-15 part, dry earthworm powder 11-19 part, oyster shell whiting 8-15 part, dried yeast powder 7-15 part.
Preferably, the feed addictive of described prevention betta splendens stigmatosis, be made up of each component of following masses number: tea 30-32 part, radix cynanchi atrati 22-24 part, purslane 21-23 part, chenopodium ambrosiodies 21-23 part, Chinese honey locust 21-23 part, moutan bark 19-21 part, oriental wormwood grass 19-21 part, cordate houttuynia 18-20 part, selfheal 18-20 part, Chinese gall 18-19 part, stone-like omphalia 17-19 part, betel nut 16-18 part, centipede 15-17 part, Chinese milk vetch 15-17 part, shepherd's purse 15-16 part, madder 14-15 part, English white 14-15 part, ginger 13-16 part, garlic 13-15 part, scorpion 12-14 part, Asiatic plantain 12-14 part, bamboo salt 11-13 part, light-coloured vinegar 11-12 part, fish oil 15-17 part, honey 11-13 part, dry earthworm powder 14-16 part, oyster shell whiting 10-12 part, dried yeast powder 10-12 part.
Preferably, the feed addictive of described prevention betta splendens stigmatosis, be made up of each component of following masses number: tea 31 parts, radix cynanchi atrati 23 parts, purslane 22 parts, chenopodium ambrosiodies 22 parts, 22 parts, Chinese honey locust, moutan bark 20 parts, oriental wormwood grass 20 parts, cordate houttuynia 19 parts, selfheal 19 parts, 18 parts, Chinese gall, stone-like omphalia 18 parts, betel nut 17 parts, centipede 16 parts, Chinese milk vetch 16 parts, 16 parts, shepherd's purse, 15 parts, madder, English white 15 parts, 14 parts, ginger, 14 parts, garlic, scorpion 13 parts, Asiatic plantain 13 parts, bamboo salt 12 parts, light-coloured vinegar 11 parts, 16 parts, fish oil, honey 12 parts, dry earthworm powder 15 parts, oyster shell whiting 11 parts, dried yeast powder 11 parts.
Another object of the present invention is to provide a kind of preparation method of feed addictive of above-mentioned prevention betta splendens stigmatosis, and the preparation method of the feed addictive of this prevention betta splendens stigmatosis comprises the following steps:
(1) take radix cynanchi atrati, purslane, chenopodium ambrosiodies and Chinese honey locust by proportioning, the gross weight of radix cynanchi atrati, purslane, chenopodium ambrosiodies and Chinese honey locust is denoted as 1 part, takes the purified water of 8 parts, at 70-80 DEG C, 3-5h is soaked in water-bath, obtains soak;
(2) boil 2h after the soak that step (1) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 4 parts in medicinal material, continue to boil 2h after being heated to boiling, merge twice fried liquid and be extract;
(3) moutan bark, oriental wormwood grass, cordate houttuynia, selfheal, Chinese gall, stone-like omphalia, betel nut, centipede, Chinese milk vetch, shepherd's purse, madder, English white, ginger, garlic, scorpion and Asiatic plantain is taken respectively by said ratio, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(4) grounds travel that extract step (2) obtained and step (3) obtain is positioned in the extraction kettle in supercritical extract instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(5) take tea, bamboo salt, light-coloured vinegar, fish oil, honey, dry earthworm powder, oyster shell whiting and dried yeast powder according to proportioning, mix with the extract of step (4) gained, obtain object.
The preparation method of described tea is: after green tea and boiling water are brewed 30 minutes with the weight ratio of 1:20, filters the filtrate of extracting gained and is tea.
The preparation method of the earthworm powder of described drying is: cleaned by earthworm fresh water, then uses distilled water flushing three times, and vacuum freeze drying, to finish-drying, is crossed 80 mesh sieves after pulverizing, obtain dry earthworm powder with pulverizer.The advantage of vacuum freeze drying is thoroughly dry, can not make the active material inactivation in earthworm body in dry process.
The using method of the feed addictive of prevention betta splendens stigmatosis of the present invention is: throw something and feed after being mixed with the weight ratio of 1:40-1:15 with commercially available basal feed by the feed addictive of prevention betta splendens stigmatosis of the present invention, preferably, the feed addictive of prevention betta splendens stigmatosis of the present invention and the weight ratio of commercially available basal feed are 1:30.
Each component effect of the present invention is resolved as follows:
Tea Polyphenols contained in the green tea that brewed tea is used has stronger astriction, has significantly suppression and killing action, have positive effect to anti-inflammatory antidiarrheal to pathogen, virus.Radix cynanchi atrati another name common vetch grass, know that micro-old, old melon gourd dipper root, mountain cigarette root, hundred swing grass, Baima common vetch, Cynanchum glaucescens, unpleasant swallowwort herb, the upright perennial herb of Asclepiadaceae, Cynanchum, coring shape, has fragrance, has except dysphoria, heat-clearing are loose swollen, the effect of myogenic pain relieving, after can managing property, dysphoria is vomitted inverse; dribbling urination, ephritis, urinary tract infections; oedema, bronchitis and rheumatic lumbocrural pain etc.Purslane is Portulacaceae annual herb plant, herb hyoscine, has clearing heat and promoting diuresis, removing toxicity for detumescence, anti-inflammatory, quenches the thirst, diuresis.Chenopodium ambrosiodies another name red pool indigo plant, Tian Xiancao, stinkweed, hookworm grass, lousewort etc., chenopodium ambrosiodies can be dispelled rheumatism, desinsection, antipruritic, can be used for roundworm disease, hookworm disease, enterobiasis, and skin eczema is controlled in external application, itch, and kills maggot.Chinese honey locust has the effect of desinsection, removing toxic substances: saponin(e element contained in Chinese honey locust is triterpene class and compound sugar, has anti-inflammatory, antiulcer, disease-resistant change effect, also has anticancer and improves the effects such as AIDS immunity.Moutan bark is the dry root skin of ranunculaceae peony, bitter, pungent, cold nature, has clearing heat and cooling blood, activates blood circulation and disperses blood clots, the effect such as reducing the asthenic fever, can be used for the treatment of to fall and flutter the pain of injury, carbuncle sore tumefacting virus.Oriental wormwood grass has another name called: Tarragon is careless, tarragon is careless, Artemisia stelleriana is careless, his power is dry, for composite family herbaceos perennial, oriental wormwood is more with fruit ear essential oil content, main component has B mono-trip alkene, capillin, oriental wormwood primary colors ketone, capillon etc., cold nature, bitter are pungent, have the effect of clearing away damp-heat, removing jaundice subcutaneous ulcer, detoxification sore treatment, drink for a long time, hepatic cholagogic, has the effect directly hindering liver and gall tumor cell proliferation.Cordate houttuynia has clearing heat and detoxicating, the carbuncle that disappears apocenosis, the effect of inducing diuresis for treating strangurtia.Folium isatidis cures mainly heat poison and sends out the diseases such as spot, erysipelas, abscess of throat, aphthae, sore and toxic.The decoction of selfheal, water-leach liquor, alcohol-water leachate and alcohol leaching liquid all can obviously reduce animal used as test blood pressure, and stem, leaf, fringe and herb all have hypotensive effect, but the effect of fringe is more obvious; The water decoction-alcohol sedimentation liquid mouse peritoneal injection of selfheal, has obvious antiinflammatory action; The decoction of selfheal all has certain inhibitory action to shigella dysenteriae, typhoid bacillus, comma bacillus, Escherichia coli, proteus, staphylococcus and Bacillus tuberculosis in vitro.Chinese gall is cold in nature, taste acid, puckery, and having astringes the lung falls the effects such as fire, relieving diarrhea with astringents, arrest sweating hemostasis, hygroscopic sore, cures mainly chronic cough of deficiency lung, rushes down protracted dysentery for a long time, the disease such as hemorrhoid of having blood in stool blood, carbuncle sore tumefacting virus, skin moisture fester.Stone-like omphalia, Chinese medicine name is the dry sclerotia of Bai Mo section fungi stone-like omphalia, can killing insect and eliminating accumulation treating tumor, is used for the treatment of tumour, taeniasis, hookworm disease, roundworm disease, abdominal pain due to enterositosis, infantile malnutrition.Betel nut is a kind of Palmae Areca, effect that betel nut has " desinsection, broken long-pending, sending down abnormally ascending is capable stagnant, and row aquation wets ", be once used to treatment tapeworm, hookworm, roundworm, around the parasitic infection such as worm, fasciolopsis.Centipede is Lu Sheng arthropod, can move back inflammation and control sore, to swell sore, can subside a swelling fast, pain relieving for inflammation.Chinese milk vetch cures mainly brokenly blood, hemostasia and promoting granulation, and profit can support the five internal organs, improve visual and auditory acuity, hot blast of dispelling, makes us spry and light, and food is not minded for a long time, mends people, can stop hot malaria, flat stomach of invigorating blood circulation.Shepherd's purse has the effect with spleen, Li Shui, hemostasis, improving eyesight, is usually used in the diseases such as treatment postpartum haemorrhage, dysentery, oedema, enteritis, gastric ulcer, cold, fever, the swollen pain of hot eyes.Madder bitter, cold in nature, return Liver Channel, have cooling blood and hemostasis, stagnation resolvation, the effect of stimulating the menstrual flow, cure mainly blood-head and tell nosebleed, metrostaxis, blood stasis is through closing, and traumatic injury, arthralgia due to wind-dampness, because madder has the effect of activating blood to promote menstruation, is commonly used to treat arthralgia due to wind-dampness clinically.English white is protoplasm poison, and the tumoricidal viability of energy, directly can kill parasite, has the effect preventing hemorrhage and mucous secretion.Ginger has bactericidal effect, and can strengthen digestion power.Garlic removing toxic substances desinsection, swelling and pain relieving, antidiarrheal stops dysentery, controls lung, expelling parasite, and also have warm spleen to warm up stomach in addition, control swollen ulcer drug, white bald tinea sore, dysentery is had loose bowels, tuberculosis pertussis, roundworm pinworm, eating accumulation, coldness and pain in the epigastrium, oedema turgor.Scorpion can anti-inflammation and sterilization, can control apoplexy, facial paralysis, hemiplegia sequelae, may be used for treating skin eczema.Asiatic plantain has another name called wheel dish, is Plantaginaceae perennial herb, not only can be medicinal, and edible, its energy reducing fever and causing diuresis, cool blood, removing toxic substances, cure mainly bladder heat stagnation, difficult urination, under stranguria with turbid discharge band, summer-heat and damp rushes down dysentery, bleeding from five sense organs or subcutaneous tissue, hematuria, the hot hot eyes of liver, abscess of throat, carbuncle sore tumefacting virus.Bamboo salt is evaporated brine day to load in triennial blue or green bamboo, and two ends are sealed with natural loess, take pine tree as fuel, the material extract after 1000 DEG C ~ 1500 DEG C high-temperature calcinations, has antibacterial, anti-inflammatory, toxopexic effect.Light-coloured vinegar have the loose stasis of blood, removing toxic substances, sterilization, antibacterial, detumescence function.Fish oil can provide energy for betta splendens, and has food calling effect.Honey can improve feed mouthfeel, can make up strength rapidly, eliminates tired out, strengthens betta splendens to the resistance of disease, and can relax bowel, also have the effect of sterilization sterilizing.Containing the multiple medicinal ingredient such as earthworm element, earthworm toxolysin, xanthine, antihistaminicum and Cobastab in earthworm body, and to betta splendens, there is food calling effect.Oyster shell whiting can be in harmonious proportion feed mouthfeel, and provides calcium source for betta splendens.The effect of dried yeast powder promotes bucket fish absorbing each component of feed and feed addictive.
In preparation method, bulk drug purified water soaking a period of time, medicinal material deliquescing, cell expansion, active ingredient is easily fried, and medicinal material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medicinal material surface, protein coagulating, the capillary channel on blocking medicinal material surface, make the moisture when decocting enter not go, active ingredient is not easily fried, affects curative effect of medication; Decoction can change the irritation effect of medicine, relatively mild act on bucket fish, promote bucket fish to the absorption of medicine; Grinding makes the starch in medicinal material, protein is separated out more fully at subsequent step; Supercritical extract, immersion, concentration step retain the active ingredient in Chinese medicine of the present invention to greatest extent.
The feed addictive of prevention betta splendens stigmatosis of the present invention, the mechanism of action according to stigmatosis, the suitable component selected, and organically combined, there is significant cooperative synergism effect, play the function of each component to greatest extent, and the component of the feed addictive of prevention betta splendens stigmatosis of the present invention is environmental protection raw material, environmental sound.
Because the fish oil in feed addictive of the present invention has food calling effect, honey can be in harmonious proportion and improve feed mouthfeel, earthworm powder delicious flavour, therefore feed addictive of the present invention can stimulate the taste bud of betta splendens, induction betta splendens is ingested and be with the addition of the basal feed of feed addictive of the present invention, in addition, again because dried yeast powder can promote bucket fish absorbing each component in feed and feed addictive, therefore betta splendens immunity and resistance can be strengthened in feed addictive of the present invention, the composition pathogen of betta splendens stigmatosis to remarkable bactericidal and bacteriostatic effect can enter in betta splendens body, and give full play to it and act on accordingly.
Compared with prior art, the invention has the beneficial effects as follows:
(1) Tea Polyphenols contained in the green tea that brewed tea is used has stronger astriction, has significantly suppression and killing action, have positive effect to anti-inflammatory antidiarrheal to pathogen, virus.Radix cynanchi atrati another name common vetch grass, know that micro-old, old melon gourd dipper root, mountain cigarette root, hundred swing grass, Baima common vetch, Cynanchum glaucescens, unpleasant swallowwort herb, the upright perennial herb of Asclepiadaceae, Cynanchum, coring shape, has fragrance, has except dysphoria, heat-clearing are loose swollen, the effect of myogenic pain relieving, after can managing property, dysphoria is vomitted inverse; dribbling urination, ephritis, urinary tract infections; oedema, bronchitis and rheumatic lumbocrural pain etc.Purslane is Portulacaceae annual herb plant, herb hyoscine, has clearing heat and promoting diuresis, removing toxicity for detumescence, anti-inflammatory, quenches the thirst, diuresis.Chenopodium ambrosiodies another name red pool indigo plant, Tian Xiancao, stinkweed, hookworm grass, lousewort etc., chenopodium ambrosiodies can be dispelled rheumatism, desinsection, antipruritic, can be used for roundworm disease, hookworm disease, enterobiasis, and skin eczema is controlled in external application, itch, and kills maggot.Chinese honey locust has the effect of desinsection, removing toxic substances: saponin(e element contained in Chinese honey locust is triterpene class and compound sugar, has anti-inflammatory, antiulcer, disease-resistant change effect, also has anticancer and improves the effects such as AIDS immunity.Moutan bark is the dry root skin of ranunculaceae peony, bitter, pungent, cold nature, has clearing heat and cooling blood, activates blood circulation and disperses blood clots, the effect such as reducing the asthenic fever, can be used for the treatment of to fall and flutter the pain of injury, carbuncle sore tumefacting virus.Oriental wormwood grass has another name called: Tarragon is careless, tarragon is careless, Artemisia stelleriana is careless, his power is dry, for composite family herbaceos perennial, oriental wormwood is more with fruit ear essential oil content, main component has B mono-trip alkene, capillin, oriental wormwood primary colors ketone, capillon etc., cold nature, bitter are pungent, have the effect of clearing away damp-heat, removing jaundice subcutaneous ulcer, detoxification sore treatment, drink for a long time, hepatic cholagogic, has the effect directly hindering liver and gall tumor cell proliferation.Cordate houttuynia has clearing heat and detoxicating, the carbuncle that disappears apocenosis, the effect of inducing diuresis for treating strangurtia.Folium isatidis cures mainly heat poison and sends out the diseases such as spot, erysipelas, abscess of throat, aphthae, sore and toxic.The decoction of selfheal, water-leach liquor, alcohol-water leachate and alcohol leaching liquid all can obviously reduce animal used as test blood pressure, and stem, leaf, fringe and herb all have hypotensive effect, but the effect of fringe is more obvious; The water decoction-alcohol sedimentation liquid mouse peritoneal injection of selfheal, has obvious antiinflammatory action; The decoction of selfheal all has certain inhibitory action to shigella dysenteriae, typhoid bacillus, comma bacillus, Escherichia coli, proteus, staphylococcus and Bacillus tuberculosis in vitro.Chinese gall is cold in nature, taste acid, puckery, and having astringes the lung falls the effects such as fire, relieving diarrhea with astringents, arrest sweating hemostasis, hygroscopic sore, cures mainly chronic cough of deficiency lung, rushes down protracted dysentery for a long time, the disease such as hemorrhoid of having blood in stool blood, carbuncle sore tumefacting virus, skin moisture fester.Stone-like omphalia, Chinese medicine name is the dry sclerotia of Bai Mo section fungi stone-like omphalia, can killing insect and eliminating accumulation treating tumor, is used for the treatment of tumour, taeniasis, hookworm disease, roundworm disease, abdominal pain due to enterositosis, infantile malnutrition.Betel nut is a kind of Palmae Areca, effect that betel nut has " desinsection, broken long-pending, sending down abnormally ascending is capable stagnant, and row aquation wets ", be once used to treatment tapeworm, hookworm, roundworm, around the parasitic infection such as worm, fasciolopsis.Centipede is Lu Sheng arthropod, can move back inflammation and control sore, to swell sore, can subside a swelling fast, pain relieving for inflammation.Chinese milk vetch cures mainly brokenly blood, hemostasia and promoting granulation, and profit can support the five internal organs, improve visual and auditory acuity, hot blast of dispelling, makes us spry and light, and food is not minded for a long time, mends people, can stop hot malaria, flat stomach of invigorating blood circulation.Shepherd's purse has the effect with spleen, Li Shui, hemostasis, improving eyesight, is usually used in the diseases such as treatment postpartum haemorrhage, dysentery, oedema, enteritis, gastric ulcer, cold, fever, the swollen pain of hot eyes.Madder bitter, cold in nature, return Liver Channel, have cooling blood and hemostasis, stagnation resolvation, the effect of stimulating the menstrual flow, cure mainly blood-head and tell nosebleed, metrostaxis, blood stasis is through closing, and traumatic injury, arthralgia due to wind-dampness, because madder has the effect of activating blood to promote menstruation, is commonly used to treat arthralgia due to wind-dampness clinically.English white is protoplasm poison, and the tumoricidal viability of energy, directly can kill parasite, has the effect preventing hemorrhage and mucous secretion.Ginger has bactericidal effect, and can strengthen digestion power.Garlic removing toxic substances desinsection, swelling and pain relieving, antidiarrheal stops dysentery, controls lung, expelling parasite, and also have warm spleen to warm up stomach in addition, control swollen ulcer drug, white bald tinea sore, dysentery is had loose bowels, tuberculosis pertussis, roundworm pinworm, eating accumulation, coldness and pain in the epigastrium, oedema turgor.Scorpion can anti-inflammation and sterilization, can control apoplexy, facial paralysis, hemiplegia sequelae, may be used for treating skin eczema.Asiatic plantain has another name called wheel dish, is Plantaginaceae perennial herb, not only can be medicinal, and edible, its energy reducing fever and causing diuresis, cool blood, removing toxic substances, cure mainly bladder heat stagnation, difficult urination, under stranguria with turbid discharge band, summer-heat and damp rushes down dysentery, bleeding from five sense organs or subcutaneous tissue, hematuria, the hot hot eyes of liver, abscess of throat, carbuncle sore tumefacting virus.Bamboo salt is evaporated brine day to load in triennial blue or green bamboo, and two ends are sealed with natural loess, take pine tree as fuel, the material extract after 1000 DEG C ~ 1500 DEG C high-temperature calcinations, has antibacterial, anti-inflammatory, toxopexic effect.Light-coloured vinegar has the function of the loose stasis of blood, removing toxic substances, detumescence.Fish oil can provide energy for betta splendens, and has food calling effect.Honey can improve feed mouthfeel, can make up strength rapidly, eliminates tired out, builds up one's resistance to disease, and can relax bowel, and also has the effect of sterilizing sterilizing.Containing the multiple medicinal ingredient such as earthworm element, earthworm toxolysin, xanthine, antihistaminicum and Cobastab in earthworm body, and to betta splendens, there is food calling effect.Oyster shell whiting can provide calcium source for betta splendens.The effect of dried yeast powder promotes bucket fish absorbing each component of feed and feed addictive.
(2) feed addictive of prevention betta splendens stigmatosis of the present invention, the mechanism of action according to stigmatosis, the suitable component selected, and organically combined, there is significant cooperative synergism effect, play the function of each component to greatest extent, and the component of the feed addictive of prevention betta splendens stigmatosis of the present invention is natural environmental-protective raw material, environmental sound.
(3) because the fish oil in feed addictive of the present invention has food calling effect, honey can be in harmonious proportion and improve feed mouthfeel, earthworm powder delicious flavour, therefore feed addictive of the present invention can stimulate the taste bud of betta splendens, induction betta splendens is ingested and be with the addition of the basal feed of feed addictive of the present invention, in addition, again because dried yeast powder can promote bucket fish absorbing each component in feed and feed addictive, therefore betta splendens immunity and resistance can be strengthened in feed addictive of the present invention, the composition pathogen of betta splendens stigmatosis to remarkable bactericidal and bacteriostatic effect can enter in betta splendens body, and can be played it fully and act on accordingly.
(4) in preparation method, bulk drug purified water soaking a period of time, medicinal material deliquescing, cell expansion, active ingredient is easily fried, and medicinal material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medicinal material surface, protein coagulating, the capillary channel on blocking medicinal material surface, make the moisture when decocting enter not go, active ingredient is not easily fried, affects curative effect of medication; Decoction can change the irritation effect of medicine, relatively mild act on bucket fish, promote bucket fish to the absorption of medicine; Grinding makes the starch in medicinal material, protein is separated out more fully at subsequent step; Supercritical extract, immersion, concentration step retain the active ingredient in Chinese medicine of the present invention to greatest extent.
Detailed description of the invention
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.
Embodiment 1
In the present invention's first detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of feed addictive preventing betta splendens stigmatosis, be made up of each component of following masses number: tea 32 parts, radix cynanchi atrati 24 parts, purslane 23 parts, chenopodium ambrosiodies 23 parts, 23 parts, Chinese honey locust, moutan bark 21 parts, oriental wormwood grass 21 parts, cordate houttuynia 20 parts, selfheal 20 parts, 19 parts, Chinese gall, stone-like omphalia 19 parts, betel nut 18 parts, centipede 17 parts, Chinese milk vetch 17 parts, 16 parts, shepherd's purse, 15 parts, madder, English white 15 parts, 16 parts, ginger, 15 parts, garlic, scorpion 14 parts, Asiatic plantain 14 parts, bamboo salt 13 parts, light-coloured vinegar 12 parts, 18 parts, fish oil, honey 13 parts, dry earthworm powder 17 parts, oyster shell whiting 12 parts, dried yeast powder 13 parts.
The preparation method of the feed addictive of above-mentioned prevention betta splendens stigmatosis comprises the following steps:
(1) take radix cynanchi atrati, purslane, chenopodium ambrosiodies and Chinese honey locust by proportioning, the gross weight of radix cynanchi atrati, purslane, chenopodium ambrosiodies and Chinese honey locust is denoted as 1 part, takes the purified water of 8 parts, at 70-80 DEG C, 3-5h is soaked in water-bath, obtains soak;
(2) boil 2h after the soak that step (1) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 4 parts in medicinal material, continue to boil 2h after being heated to boiling, merge twice fried liquid and be extract;
(3) moutan bark, oriental wormwood grass, cordate houttuynia, selfheal, Chinese gall, stone-like omphalia, betel nut, centipede, Chinese milk vetch, shepherd's purse, madder, English white, ginger, garlic, scorpion and Asiatic plantain is taken respectively by said ratio, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(4) grounds travel that extract step (2) obtained and step (3) obtain is positioned in the extraction kettle in supercritical extract instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(5) take tea, bamboo salt, light-coloured vinegar, fish oil, honey, dry earthworm powder, oyster shell whiting and dried yeast powder according to proportioning, mix with the extract of step (4) gained, obtain object.
The preparation method of described tea is: after green tea and boiling water are brewed 30 minutes with the weight ratio of 1:20, filters the filtrate of extracting gained and is tea.
The preparation method of the earthworm powder of described drying is: cleaned by earthworm fresh water, then uses distilled water flushing three times, and vacuum freeze drying, to finish-drying, is crossed 80 mesh sieves after pulverizing, obtain dry earthworm powder with pulverizer.
Embodiment 2
In the present invention's second detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of feed addictive preventing betta splendens stigmatosis, be made up of each component of following masses number: tea 35 parts, radix cynanchi atrati 26 parts, purslane 25 parts, chenopodium ambrosiodies 25 parts, 25 parts, Chinese honey locust, moutan bark 23 parts, oriental wormwood grass 23 parts, cordate houttuynia 23 parts, selfheal 22 parts, 21 parts, Chinese gall, stone-like omphalia 22 parts, betel nut 20 parts, centipede 20 parts, Chinese milk vetch 19 parts, 18 parts, shepherd's purse, 19 parts, madder, English white 19 parts, 19 parts, ginger, 13 parts, garlic, scorpion 16 parts, Asiatic plantain 17 parts, bamboo salt 14 parts, light-coloured vinegar 15 parts, 20 parts, fish oil, honey 15 parts, dry earthworm powder 19 parts, oyster shell whiting 15 parts, dried yeast powder 15 parts.
The preparation method of the feed addictive of the prevention betta splendens stigmatosis of the present embodiment is with embodiment 1.
Embodiment 3
In the present invention's the 3rd detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of feed addictive preventing betta splendens stigmatosis, be made up of each component of following masses number: tea 33 parts, radix cynanchi atrati 22 parts, purslane 23 parts, chenopodium ambrosiodies 21 parts, 21 parts, Chinese honey locust, moutan bark 19 parts, oriental wormwood grass 19 parts, cordate houttuynia 18 parts, selfheal 18 parts, 19 parts, Chinese gall, stone-like omphalia 17 parts, betel nut 16 parts, centipede 18 parts, Chinese milk vetch 15 parts, 15 parts, shepherd's purse, 14 parts, madder, English white 17 parts, 13 parts, ginger, 17 parts, garlic, scorpion 17 parts, Asiatic plantain 12 parts, bamboo salt 16 parts, light-coloured vinegar 11 parts, 17 parts, fish oil, honey 14 parts, dry earthworm powder 16 parts, oyster shell whiting 13 parts, dried yeast powder 12 parts.
The preparation method of the feed addictive of the prevention betta splendens stigmatosis of the present embodiment is with embodiment 1.
Embodiment 4
In the present invention's the 4th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of feed addictive preventing betta splendens stigmatosis, be made up of each component of following masses number: tea 30 parts, radix cynanchi atrati 20 parts, purslane 21 parts, chenopodium ambrosiodies 19 parts, 19 parts, Chinese honey locust, moutan bark 17 parts, oriental wormwood grass 17 parts, cordate houttuynia 15 parts, selfheal 16 parts, 18 parts, Chinese gall, stone-like omphalia 20 parts, betel nut 14 parts, centipede 15 parts, Chinese milk vetch 13 parts, 13 parts, shepherd's purse, 17 parts, madder, English white 14 parts, 18 parts, ginger, 10 parts, garlic, scorpion 12 parts, Asiatic plantain 9 parts, bamboo salt 11 parts, light-coloured vinegar 8 parts, 15 parts, fish oil, honey 11 parts, dry earthworm powder 14 parts, oyster shell whiting 10 parts, dried yeast powder 10 parts.
The preparation method of the feed addictive of the prevention betta splendens stigmatosis of the present embodiment is with embodiment 1.
Embodiment 5
In the present invention's the 5th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of feed addictive preventing betta splendens stigmatosis, be made up of each component of following masses number: tea 27 parts, radix cynanchi atrati 20 parts, purslane 19 parts, chenopodium ambrosiodies 20 parts, 19 parts, Chinese honey locust, moutan bark 17 parts, oriental wormwood grass 17 parts, cordate houttuynia 21 parts, selfheal 16 parts, 15 parts, Chinese gall, stone-like omphalia 14 parts, betel nut 15 parts, centipede 12 parts, Chinese milk vetch 15 parts, 13 parts, shepherd's purse, 12 parts, madder, English white 11 parts, 10 parts, ginger, 10 parts, garlic, scorpion 9 parts, Asiatic plantain 11 parts, bamboo salt 8 parts, light-coloured vinegar 9 parts, 12 parts, fish oil, honey 9 parts, dry earthworm powder 11 parts, oyster shell whiting 8 parts, dried yeast powder 7 parts.
The preparation method of the feed addictive of the prevention betta splendens stigmatosis of the present embodiment is with embodiment 1.
Embodiment 6
In the present invention's the 6th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of feed addictive preventing betta splendens stigmatosis, be made up of each component of following masses number: tea 31 parts, radix cynanchi atrati 23 parts, purslane 22 parts, chenopodium ambrosiodies 22 parts, 22 parts, Chinese honey locust, moutan bark 20 parts, oriental wormwood grass 20 parts, cordate houttuynia 19 parts, selfheal 19 parts, 18 parts, Chinese gall, stone-like omphalia 18 parts, betel nut 17 parts, centipede 16 parts, Chinese milk vetch 16 parts, 16 parts, shepherd's purse, 15 parts, madder, English white 15 parts, 14 parts, ginger, 14 parts, garlic, scorpion 13 parts, Asiatic plantain 13 parts, bamboo salt 12 parts, light-coloured vinegar 11 parts, 16 parts, fish oil, honey 12 parts, dry earthworm powder 15 parts, oyster shell whiting 11 parts, dried yeast powder 11 parts.
The preparation method of the feed addictive of the prevention betta splendens stigmatosis of the present embodiment is with embodiment 1.
Experimental result
Preventive effect is tested
Choose 60 aquariums, healthy betta splendens 60, healthy betta splendens is assigned at random 60 aquariums, each aquarium 1 betta splendens, every 10 aquariums are a processed group, control group and experimental group establish respectively 3 parallel.With group of normally feeding for blank group, in experimental group, the feed addictive of embodiment 6 and the weight ratio of basal feed are 1:30, after throwing something and feeding continuously 60 days, the betta splendens that 1 is suffered from stigmatosis is added again respectively in each aquarium, to add after ill betta splendens 5 days, record experimental result, experimental result is in table 1:
Table 1 embodiment of the present invention 6 is in the effect situation of prevention betta splendens stigmatosis
From table 1, the feed addictive of prevention betta splendens stigmatosis of the present invention effectively can reduce the incidence rate of betta splendens stigmatosis, can improve the immunity of bucket fish, have good prevention effect to stigmatosis.
Bacteriostatic test checks
Adopt furazolidone to do control group, the feed addictive of the prevention betta splendens stigmatosis of the embodiment of the present invention 6 is that experimental group carries out antibacterial inspection.
The preparation of culture medium: take in the water-soluble 800mL of beef extract 5g, adds peptone 10g and dissolves, and adds casein 34g and dissolves, then adds NaCl5g dissolving, is adjusted to pH7.6 ~ 7.8, is diluted to 1000mL.Add agar powder 18g, autoclaving 15min, when being cooled to 40 DEG C ~ 50 DEG C, a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices, solidifies, and the 4 DEG C of refrigerators placing no bacteria pollution are for subsequent use.
Bacterial classification is: point-like Aerononas punctata.
Control group antibacterial liquid is prepared: be dissolved in respectively in 10ml aseptic deionized water by 1 part of furazolidone, diluted respectively by liquor strength, with stoste than being 1:2,1:4,1:8,1:16,1:32.
Experimental group antibacterial liquid is prepared: be dissolved in 10ml aseptic deionized water by the feed addictive of the prevention betta splendens stigmatosis of the embodiment of the present invention 6, diluted respectively by liquor strength, with stoste than being 1:2,1:4,1:8,1:16,1:32.To the fungistatic effect of point-like Aerononas punctata in table 2:
The bactericidal and bacteriostatic effect of the feed addictive of table 2 prevention betta splendens of the present invention stigmatosis
From table 2, the pathogen point-like Aerononas punctata of feed addictive to stigmatosis of the prevention betta splendens stigmatosis of embodiments of the invention 6 has good bactericidal and bacteriostatic effect.
The above is only the preferred embodiment of the present invention, is noted that for those skilled in the art, and under the premise without departing from the principles of the invention, can also make some improvement, these improvement should be considered as protection scope of the present invention.
Claims (8)
1. one kind is prevented the feed addictive of betta splendens stigmatosis, it is characterized in that: be made up of each component of following masses number: tea 27-35 part, radix cynanchi atrati 20-26 part, purslane 19-25 part, chenopodium ambrosiodies 19-25 part, Chinese honey locust 19-25 part, moutan bark 17-23 part, oriental wormwood grass 17-23 part, cordate houttuynia 15-23 part, selfheal 16-22 part, Chinese gall 15-21 part, stone-like omphalia 14-22 part, betel nut 14-20 part, centipede 12-20 part, Chinese milk vetch 13-19 part, shepherd's purse 13-18 part, madder 12-19 part, English white 11-19 part, ginger 10-19 part, garlic 10-17 part, scorpion 9-17 part, Asiatic plantain 9-17 part, bamboo salt 8-16 part, light-coloured vinegar 8-15 part, fish oil 12-20 part, honey 9-15 part, dry earthworm powder 11-19 part, oyster shell whiting 8-15 part, dried yeast powder 7-15 part.
2. the feed addictive of prevention betta splendens stigmatosis according to claim 1, it is characterized in that: the feed addictive of described prevention betta splendens stigmatosis, be made up of each component of following masses number: tea 30-32 part, radix cynanchi atrati 22-24 part, purslane 21-23 part, chenopodium ambrosiodies 21-23 part, Chinese honey locust 21-23 part, moutan bark 19-21 part, oriental wormwood grass 19-21 part, cordate houttuynia 18-20 part, selfheal 18-20 part, Chinese gall 18-19 part, stone-like omphalia 17-19 part, betel nut 16-18 part, centipede 15-17 part, Chinese milk vetch 15-17 part, shepherd's purse 15-16 part, madder 14-15 part, English white 14-15 part, ginger 13-16 part, garlic 13-15 part, scorpion 12-14 part, Asiatic plantain 12-14 part, bamboo salt 11-13 part, light-coloured vinegar 11-12 part, fish oil 15-17 part, honey 11-13 part, dry earthworm powder 14-16 part, oyster shell whiting 10-12 part, dried yeast powder 10-12 part.
3. the feed addictive of prevention betta splendens stigmatosis according to claim 1, it is characterized in that: the feed addictive of described prevention betta splendens stigmatosis, be made up of each component of following masses number: tea 31 parts, radix cynanchi atrati 23 parts, purslane 22 parts, chenopodium ambrosiodies 22 parts, 22 parts, Chinese honey locust, moutan bark 20 parts, oriental wormwood grass 20 parts, cordate houttuynia 19 parts, selfheal 19 parts, 18 parts, Chinese gall, stone-like omphalia 18 parts, betel nut 17 parts, centipede 16 parts, Chinese milk vetch 16 parts, 16 parts, shepherd's purse, 15 parts, madder, English white 15 parts, 14 parts, ginger, 14 parts, garlic, scorpion 13 parts, Asiatic plantain 13 parts, bamboo salt 12 parts, light-coloured vinegar 11 parts, 16 parts, fish oil, honey 12 parts, dry earthworm powder 15 parts, oyster shell whiting 11 parts, dried yeast powder 11 parts.
4. a preparation method for the feed addictive of the prevention betta splendens stigmatosis according to any one of claim 1-3, is characterized in that: comprise the following steps:
(1) take radix cynanchi atrati, purslane, chenopodium ambrosiodies and Chinese honey locust by proportioning, the gross weight of radix cynanchi atrati, purslane, chenopodium ambrosiodies and Chinese honey locust is denoted as 1 part, takes the purified water of 8 parts, at 70-80 DEG C, 3-5h is soaked in water-bath, obtains soak;
(2) boil 2h after the soak that step (1) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 4 parts in medicinal material, continue to boil 2h after being heated to boiling, merge twice fried liquid and be extract;
(3) moutan bark, oriental wormwood grass, cordate houttuynia, selfheal, Chinese gall, stone-like omphalia, betel nut, centipede, Chinese milk vetch, shepherd's purse, madder, English white, ginger, garlic, scorpion and Asiatic plantain is taken respectively by said ratio, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(4) grounds travel that extract step (2) obtained and step (3) obtain is positioned in the extraction kettle in supercritical extract instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(5) take tea, bamboo salt, light-coloured vinegar, fish oil, honey, dry earthworm powder, oyster shell whiting and dried yeast powder according to proportioning, mix with the extract of step (4) gained, obtain object.
5. the preparation method of the feed addictive of prevention betta splendens stigmatosis according to claim 4, it is characterized in that: the preparation method of described tea is: after green tea and boiling water are brewed 30 minutes with the weight ratio of 1:20, filter the filtrate of extracting gained and be tea.
6. the preparation method of the feed addictive of prevention betta splendens stigmatosis according to claim 4, it is characterized in that: the preparation method of the earthworm powder of described drying is: cleaned by earthworm fresh water, use distilled water flushing again three times, vacuum freeze drying is to finish-drying, cross 80 mesh sieves after pulverizing with pulverizer, obtain dry earthworm powder.
7. a using method for the feed addictive of the prevention betta splendens stigmatosis according to any one of claim 1-3, is characterized in that: throw something and feed after being mixed with the weight ratio of 1:40-1:15 with commercially available basal feed by the feed addictive of prevention betta splendens stigmatosis of the present invention.
8. a using method for the feed addictive of prevention betta splendens stigmatosis according to claim 7, is characterized in that: the feed addictive of prevention betta splendens stigmatosis of the present invention and the weight ratio of commercially available basal feed are 1:30.
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| CN201510724226.0A CN105249003A (en) | 2015-10-29 | 2015-10-29 | Feed additive for preventing stigmatosis of Siamese fighting fish and preparation method thereof |
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| CN201510724226.0A CN105249003A (en) | 2015-10-29 | 2015-10-29 | Feed additive for preventing stigmatosis of Siamese fighting fish and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107307231A (en) * | 2017-07-20 | 2017-11-03 | 通化师范学院 | It is a kind of to prevent Chinese herbal feed additive of golden trout stigmatosis and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101214362A (en) * | 2007-12-27 | 2008-07-09 | 李勇军 | Preparations for treating bacterial infection disease in fishes and preparation |
| CN104970193A (en) * | 2015-07-01 | 2015-10-14 | 青岛文创科技有限公司 | Feed suitable for betta to eat |
-
2015
- 2015-10-29 CN CN201510724226.0A patent/CN105249003A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101214362A (en) * | 2007-12-27 | 2008-07-09 | 李勇军 | Preparations for treating bacterial infection disease in fishes and preparation |
| CN104970193A (en) * | 2015-07-01 | 2015-10-14 | 青岛文创科技有限公司 | Feed suitable for betta to eat |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107307231A (en) * | 2017-07-20 | 2017-11-03 | 通化师范学院 | It is a kind of to prevent Chinese herbal feed additive of golden trout stigmatosis and application thereof |
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Application publication date: 20160120 |