CN108066518A - A kind of dog Chinese medicine bath lotion composition and preparation method thereof - Google Patents

Abstract

The invention discloses a Chinese medicine bath liquid composition for dogs and a preparation method thereof, which comprises the following components in the raw materials of Chinese herbal medicines in parts by weight: 10-15 parts of Sophora flavescens, 3-5 parts of cicada slough, 6-10 parts of almond, 20‑30 parts of wormwood, 10‑15 parts of cnidium, 5‑8 parts of Hedyotis diffusa, 5‑6 parts of forsythia, 10‑15 parts of mint, 10‑15 parts of aloe vera, 3‑5 parts of propolis. The present invention selects various traditional Chinese medicine components, which have less toxic and side effects compared with some western medicines or hormone drugs, have a wide range of insecticidal and bactericidal, and are not easy to cause drug resistance of pathogenic bacteria and parasites. The treatment is not destroyed by the stomach and intestines, but directly acts on the skin, and the drug effect acts on the skin surface of the whole body and the affected area. It is faster than oral medicine, without any side effects, and will not increase the burden on the liver.

Description

A kind of traditional Chinese medicine bath liquid composition for dogs and preparation method thereof

technical field

The invention belongs to the technical field of prevention and treatment of animal diseases, and in particular relates to a Chinese medicine bath liquid composition for dogs and a preparation method thereof.

Background technique

Dogs have long hair on the surface, often roll on the ground, grass and other places where there are many germs and parasites, and the hair is not cleaned frequently, so they are prone to skin diseases such as canine pyoderma, skin fungal infection and ectoparasite infection.

Canine pyoderma is a kind of skin inflammation in which the dermis and subcutaneous tissue are necrotic to form pus when accompanied by bacterial infection. It is different from exudative or suppurative superficial dermatitis. Several types of tropophilic granuloma. Impetigo is caused by Staphylococcus, Streptococcus infection and the formation of pustules, small blisters and other pathological changes in the epidermis. It is more common in the abdomen of dogs. It is small and white, and sometimes pus oozes out and dries to form scabs. Small dogs can be seen around the mouth and feet. Weak dogs are more likely to develop the disease. The treatment can be treated with iodine tincture or antibiotics with better penetration after debridement. For larger areas of inflammation, antibiotics need to be used systemically. Furuncle is a deep hair follicle abscess, usually accompanied by dermal or subcutaneous abscess, and some ulcerate and ooze pus and blood. Furuncle is more common in dogs with rough hair, such as Doberman and Great Dane, etc. The dog’s body is often compressed In the area of friction, such as the chest, limbs, especially the protruding bones, there are multiple furuncles. In the later stage, hyperkeratosis of the perigroup tissue may form callus purulent disease, and the damaged hair follicles will become pus stubble. Lactophilic granuloma is a chronic skin ulcer caused by dog licking and biting, mostly secondary to previous inflammation or itching. The main clinical changes are: wound redness, hair loss, tissue exudation and inert epithelial margin. For small ulcers, corticosteroids can be used topically. Wear a mask to the dog to avoid the dog's constant licking, and use cryotherapy to destroy sensitive nerve endings and excessively proliferated granulation tissue to promote wound healing, increase the dog's activity, and reduce the dog's licking.

Ringworm is a common skin disease caused by fungi. It often occurs on the face, trunk, limbs, and tail of dogs. There are round or oval ringworm spots covered with gray scales, which can be seen purple when irradiated by ultraviolet light. fluorescent spots. The coat color of the affected dog will become rough, and the fur of the tinea spot part will fall off and break or fall off one by one. The infected dog will feel extremely itchy and scratch repeatedly, so the affected part will form alopecia areata. Ringworm can also be transmitted to humans, causing itching and peeling of the skin. Ringworm is more common in malnourished and weak and sickly dogs. Increasing the intake of nutrition, especially B vitamins, is conducive to the prevention of the disease and the recovery of infected animals. There are 2 kinds of fungi that infect dogs, namely Microsporum canis and Trichophyton mentagrophytes, 98% are Microsporum canis, and as the disease evolves, it becomes an irritating dry circular macule (ringworm). At present, antifungal drugs such as griseofulvin and clotrimazole are mainly used for the treatment of canine tinea. However, due to the strong resistance of the spores to heat and disinfectant liquid, it is not easy to kill them. Therefore, once the disease occurs, Difficult to treat completely.

Canine mite, also known as canine scabies, is a common dog ectoparasitic infection, commonly known as "mangy dog disease", which is caused by parasitism of scabies mite or canine ear itch mite. For dogs with poor ventilation and feeding conditions, live worms can be seen in microscopic examination. Canine mite disease mostly occurs on the head, neck, and tail. The symptoms of puppies are severe. It first occurs on the bridge of the nose, cheeks, ears, and axils, and then spreads to the whole body. At first, the skin becomes red and small red nodules appear. Blisters form, and after the blisters are ruptured, a thick yellow oily exudate flows out, and the exudate dries to form a fish-like scab. If small pustules can appear after the secondary infection, as the disease continues to develop, the lesion will continue to expand and may spread to the whole body. Due to the stimulation of mites secreting toxins, the lesion is characterized by severe itching, nodules, rapid scabs, and hair loss at the lesion site, causing the dog to become thin, inconvenient to move, and even die. Due to the special parasitic parts of the parasites and the fast transmission speed, the disease is more difficult to prevent and treat, seriously affecting the growth, development and production of pets, and causing great harm to the breeding industry of pet dogs and cats. , Because scabies mites can infect humans, pet dog and cat mites will bring potential harm and infection to breeders and affect human health. At present, the drug treatment uses ivermectin (Haumil) 1% concentration, 0.5-1 mg/kg body weight, subcutaneous injection on the back, once every 6-7 months, 2-3 times as a course of treatment. Use western medicine treatment alone at present, effect is not exact, and sick dog likes to dawdle skin, causes skin damage, secondary bacterial infection, and disease shows effect repeatedly, and itching can not be eliminated.

Based on the above, for dog skin diseases caused by bacteria, fungi, parasites, etc., some western medicines and even hormone drugs are currently used. And, when these skin diseases come on, often have caused the infection of a certain degree, have formed greater harm. At present, the use of traditional Chinese medicine in the prevention and treatment of canine diseases is less, and the use of my country's rich resources of Chinese herbal medicines to develop Chinese medicine preparations suitable for the prevention and treatment of canine skin diseases has always controlled the incidence of canine skin diseases. It provides new ideas for the clinical treatment of similar skin diseases, and also has a very broad market prospect.

Contents of the invention

Aiming at the deficiencies of the prior art, the present invention provides a traditional Chinese medicine bath liquid composition for dogs and its preparation method, which can prevent and treat various canine skin diseases, reduce the incidence of canine skin diseases, and prevent diseases from being cured After repeated attacks.

In order to achieve the above object, the technical solution of the present invention is a Chinese medicine bath liquid composition for dogs and its preparation method, comprising the following components in the raw materials of Chinese herbal medicine in parts by weight: 10-15 parts of Sophora flavescens, 3-5 parts of cicada slough , 6-10 parts of almonds, 20-30 parts of wormwood, 10-15 parts of cnidium, 5-8 parts of Hedyotis diffusa, 5-6 parts of forsythia, 10-15 parts of mint, 10-15 parts of aloe vera, propolis 3-5 servings.

Preferably, 6-8 parts of Artemisia annua are also included.

Preferably, 10-13 parts of garlic cloves are also included.

Preferably, 2-3 parts of green salt are also included.

Preferably, it also includes 0.5-2 parts of essential oil; said essential oil is one or more of lemongrass essential oil, torreya essential oil, and tea tree essential oil.

The essential oil is formed by mixing lemongrass essential oil, torreya essential oil and tea tree essential oil in a weight ratio of 1:2:1.5.

The present invention also provides a preparation method of the traditional Chinese medicine bath liquid composition for dogs, comprising the following steps:

(1) Raw material processing: Dry Sophora flavescens, almonds, mint, aloe, propolis, Artemisia annua, garlic cloves and wormwood, respectively, crush them, sieve them, then add green salt and mix well to obtain a mixture;

(2) water leaching: heat the mixture of step (1) gained after adding water, carry out leaching under the assisted action of ultrasonic wave, after leaching finishes, filter to obtain filtrate I; Add water to filter residue and carry out ultrasonic assisted leaching, filter according to the same method , repeat 2-3 times to obtain filtrate II; combine filtrate I and filtrate II to obtain water extract;

(3) Ultrasonic countercurrent extraction: add 55-60% ethanol to the extraction filter residue of step (2) gained, countercurrent extraction under the ultrasonic assisted action, filter to obtain extract I; Filter residue adds 55-60% ethanol of equal weight according to Ultrasonic-assisted countercurrent extraction and filtration are performed according to the method, and the extraction solution II is obtained by repeating 1-2 times; the countercurrent extraction solution is obtained by combining the extraction solution I and the extraction solution II;

(4) Extraction by decocting in water: take cicada slough, cnidium, hedyotis diffusa and forsythia, add 5-8 times the weight of water to soak for 1-2 hours, then boil with high heat, turn to slow fire and fry for 1.5-2 hours, filter, Collect the decoction I; boil the dregs with 2-4 times the weight of water, boil over high heat, turn to simmer for 0.5-1 hour, filter, and collect the decoction II; combine the decoction I and decoction II to obtain decoction cooking liquid;

(5) Mixing and concentration: After cooling the water decoction prepared in step (4) to below 50°C, add the water extract prepared in step (2) and the countercurrent extract prepared in step (3) and mix evenly, then Evaporate and concentrate to obtain thick liquid of traditional Chinese medicine;

(6) Disinfection and sterilization: sterilize and sterilize the traditional Chinese medicine thick liquid prepared in the step (5), add essential oils to mix, and aseptically pack into bags or cans to obtain the finished product.

Preferably, in step (1), the drying temperature is 35-50°C, and the drying time is 12-24h; after crushing, pass through a 60-mesh sieve; in step (2), the mixture and water have a mass-volume ratio of 1 : 3-5 solid-liquid ratio mixed, then heated to 40-50 ° C, under the assisted action of 700-900W ultrasonic waves, leached for 20-30min, after leaching, 1-3 layers of 80-100 mesh gauze were filtered to obtain Filtrate I; the filter residue is mixed with water according to a solid-to-liquid ratio of 1:2-3 by mass to volume.

Preferably, in step (3), the leaching filter residue is mixed with 55-60% ethanol according to a solid-to-liquid ratio of 1:2-3 in mass volume ratio, and after countercurrent extraction for 50-80 min under the assisted action of 1000-1200W ultrasonic waves , 1-3 layers of 80-100 mesh gauze are filtered to obtain the extract I.

Preferably, in step (5), the temperature of low-temperature evaporation is 40-45°C; in step (6), the disinfection and sterilization method is one of ultraviolet disinfection or 20KGy60Co disinfection.

In the present invention, the effect of each raw material is as follows:

Sophora flavescens tastes bitter, cold in nature. GUIXIN, liver, stomach, large intestine, urinary bladder channel. It is the dried root of the leguminous plant Sophora flavescens. Have heat-clearing and damp-drying, desinsection-relieving itching, local antibacterial and anti-inflammatory effect, for dysentery with heat, blood in stool. Jaundice with urinary retention, red and white vaginal discharge, vulvar swelling and itching, eczema, eczema, skin itching, mange and leprosy; external treatment of trichomonas vaginitis.

Cicada slough is sweet in taste and cold in nature. Return lung, Liver Channel. It is the shell shed by the nymphs of the cicadae insects, the black grasshopper, when they emerge. It has the effects of dispersing wind and removing heat, relieving sore throat, clearing rash, removing nebula, and relieving spasm. For anemopyretic cold, sore throat, hoarseness, opaque measles, rubella pruritus, convulsions, convulsions, and tetanus.

(Raw) almonds are bitter and slightly warm. Laetrile has anti-mutagenic effects and can reduce the number of micronucleated polychromatic red blood cells caused by Analgin, Metronidazole, Mitomycin C, etc. Almond oil also has deworming and bactericidal effects. In vitro tests have killing effects on human roundworms and earthworms, and have antibacterial effects on typhoid and paratyphoid bacillus. Almonds also have the effect of resisting pinworm and trichomonas infection and treating aplastic anemia.

Artemisia argyi is bitter, pungent and warm in nature, enters the spleen, liver and kidney, and has the effects of regulating menstruation and stopping bleeding, preventing miscarriage and stopping collapse, dispelling cold and dehumidifying. Modern experimental studies have proved that Artemisia argyi has antibacterial and antiviral effects; hemostatic and anticoagulant effects; sedative and antiallergic effects; liver protection and choleretic effects, etc. Wormwood has a special fragrance, which has the effect of repelling mosquitoes. Therefore, the ancients often hung wormwood in front of the door to avoid evil spirits and drive away mosquitoes. Modern pharmacology finds that Folium Artemisiae Argyi volatile oil content is many, and 1.8-cineole (accounting for more than 50%), other has α-thujone, sesquiterpene alcohol and its ester. Air-dried leaves contain 10.13% minerals, 2.59% fat, 25.85% protein, and vitamins A, B1, B2, C, etc.

Fructus Cnidii is the dry ripe fruit of Umbelliferae Cnidium, pungent, bitter, warm, slightly poisonous, has the effects of drying dampness, expelling wind, killing parasites, relieving itching, warming the kidney and strengthening yang.

Hedyotis diffusa, its patent medicine tastes bitter, light, cold in nature. The main effects are clearing away heat and detoxification, eliminating pain and stagnation, diuresis and dehumidification. It is especially good at treating various types of inflammation, and has a good therapeutic effect on boils and sores. In clinical practice, it has been found that Hedyotis diffusa can treat many diseases if it is properly compatible.

Forsythia is cool in nature, bitter in taste, and non-toxic. The main functions are clearing away heat, detoxification, dispelling stagnation, and detumescence. Control warm and hot, erysipelas, macule, carbuncle sore swelling poison, scrofula, urination stranguria.

Peppermint is a pungent and cool sweating and antipyretic medicine, which can treat diseases such as influenza, headache, red eyes, body heat, sore throat and gums. External application can cure neuralgia, itchy skin, rash and eczema, etc.

Aloe is bitter and cold in nature and flavor. The main effects are purging fire, detoxifying, removing blood stasis, and killing insects. Mainly used for red eyes, constipation, white turbidity, hematuria, convulsions in children, malnutrition, burns and scalds, women's amenorrhea, hemorrhoids, scabies, carbuncle, furuncle, swollen poison, bruises. Modern pharmacology has found that it has good bactericidal effect, anti-inflammatory effect, moisturizing and beautifying effect, invigorating the stomach and draining effect, strengthening the heart and promoting blood circulation, immunity and regeneration, immunity and anti-tumor effect, detoxification effect, anti-aging effect, analgesia, Sedative effect, sun protection effect.

Propolis is flat in nature, bitter, pungent, and slightly sweet. It has the effects of moisturizing skin and promoting muscle growth, reducing inflammation and relieving pain, and can treat gastric ulcers, oral ulcers, burns, skin cracks, and radiation protection. It is taken orally to nourish weakness, dissolve turbid fat, and quench thirst; externally used to detoxify and reduce swelling. Oral administration is used for physical deficiency and premature aging, hyperlipidemia, diabetes; external treatment is used for chapped skin, burns and scalds. Propolis contains more than 300 kinds of nutrients in 20 categories. Among the components of propolis, the most representative active substances are quercetin in flavonoids, phenethyl caffeate in terpenoids and organic acids. In addition to flavonoids, propolis is also rich in amino acids, microorganisms, Essential oils, terpenoids, organic acids, flavanols, alcohols, phenols, aldehydes, ketones, esters, ethers, enzymes and inorganic salts are like a natural "medicine storehouse".

Artemisia annua is pungent in nature and flavor, cool, non-toxic, and cold. The main effect is to clear away heat and relieve malaria, dispel wind and relieve itching, and its leaf juice can be applied to malignant sores, scabies and poisonous insect bites. Artemisia annua is used as medicine for clearing heat, relieving heat, cutting off malaria, cooling blood, external medicine, spices, and livestock feed. Artemisia annua contains volatile oil, and contains artemisinin, flavonoids and so on. Artemisinin in Artemisia annua is the main active ingredient in antimalarial.

Garlic is warm in nature and flavor, pungent and sweet. Can disperse carbuncle, swollen, nightmare sore, remove pathogenic wind, kill poisonous gas. Its main functions are to detoxify and kill insects, reduce swelling and relieve pain, relieve diarrhea and dysentery, cure lungs, expel insects, and warm the spleen and stomach. Cough, roundworm pinworms, food stagnation, cold pain in the abdomen, edema and fullness, promoting qi and eliminating accumulation, killing parasites and detoxifying. Sulfur-containing compounds in garlic (trithiodipropylene, also known as allicin, garlic oil) have extremely strong antibacterial and anti-inflammatory effects, and can inhibit and kill a variety of cocci, bacilli, fungi and viruses, which is currently discovered One of the strongest antibacterial effects among natural plants.

Green Salt, nature and flavor: salty, cold, has the effect of cooling blood and improving eyesight, and is used for treating hematemesis, epistaxis, hematuria, externally treating symptoms such as conjunctival congestion, swelling and pain, toothache. Green salt is also called "lake salt" or "rock salt".

Lemongrass essential oil is an essential oil extracted from lemongrass. It has very good anti-depression, antibacterial, sterilizing, flatulence, deodorizing, helping digestion, diuretic, fungicidal, prolactin, insecticidal, disease prevention, motivation, tonic Body and other effects. It can effectively repel insects, keep fleas and pests away from animals, and the deodorizing function can keep animals smelling good.

The inhibitory effect of Torreya essential oil on fungi is better than that on bacteria. Studies have found that the inhibitory effect on bacteria is time-sensitive, and the maximum inhibitory effect will appear around 48 hours. Moreover, animal experiments have shown that Torreya essential oil has a good repellent effect on Aedes albopictus, and can be used as a mosquito repellent; while the ethanol extract of Torreya, like the root extract of roses, has anti-inflammatory effects, which confirms the Chinese folk medicinal Torreya. Anti-inflammatory and analgesic claims.

Tea tree essential oil can effectively fight against a variety of bacteria. Studies have shown that 0.25% concentration of tea tree essential oil can inhibit 90% of E. coli, 0.5% concentration of tea tree essential oil can inhibit the reproduction of Staphylococcus aureus, and even against various antibiotic Superbugs that have developed resistance: Methicillin-resistant blond wines can kill them.

Compared with prior art, the beneficial technical effect of the present invention:

(1) The traditional Chinese medicine bath composition for dogs of the present invention not only simply mixes the Chinese medicine raw materials with relevant effects, but combines its medicinal mechanism to make the components of the traditional Chinese medicine synergistic, thereby exerting the greatest medicinal effect. effect. The present invention has the effects of antibacterial, anti-inflammatory, heat-clearing and detoxifying. Compared with the special medicine of western medicine, it can only treat skin diseases in one aspect, and it is easy to relapse. The traditional Chinese medicine bath composition prepared by the present invention is widely used in medicine and can Effectively treat canine contagious skin diseases caused by bacteria, fungi and parasites, the treatment effect is remarkable and recurrence is not easy.

(2) For healthy dogs, a good preventive effect can also be achieved by reducing the concentration (that is, increasing the dilution factor) of the traditional Chinese medicine bath composition of the present invention.

(3) The selected medicines of the present invention have the effects of moisturizing the skin, clearing away heat and detoxifying, expelling wind and relieving itching. During the medicated bath, the water temperature can make the hair orifices open, thereby acting on the epidermis and even the lesions under the dermis, and the open pores It can make the active ingredients of the medicine enter the body more easily, so the medicinal effect is more obvious and rapid, and it can radically cure various skin infectious diseases of dogs. Moreover, after the dog medicated bath or shower, the lesion site will not feel itchy, so the dog will not scratch the lesion frequently due to excessive itching or pain, thereby avoiding the lesion from becoming more serious, reducing the pain of the dog, and speeding up the recovery process. the treatment process.

(4) The essential oil in the present invention can not only cover the smell of the medicine, but also provide fresh fragrance; and the compound essential oil is composed of lemongrass essential oil, Chinese torreya essential oil, and tea tree essential oil according to a certain ratio, and the three have synergistic effect, so that the medicine of the present invention can prevent and better treatment.

(5) In the preparation process of the present invention, by using different processing and extraction methods for different Chinese medicine raw materials, the target components in each Chinese medicine raw material are fully extracted, and in some processes such as drying, extraction, concentration and disinfection , all control the temperature, so that some medicinal ingredients remain in the state of active ingredients, so as to ensure that the therapeutic effect is remarkable.

(6) Compared with oral medicine, the present invention has quick effect, and it is not easy to smear all lesion sites compared with skin smear cream (some diseased sites are invisible to the naked eye), and can act on all skin lesion sites.

Based on the above, the present invention selects various components of traditional Chinese medicines, which are less toxic and side effects than some western medicines or hormone drugs, have a wide range of insecticidal and bactericidal, and are not easy to cause drug resistance of pathogenic bacteria and parasites. It does not go through gastrointestinal damage, but directly acts on the skin, and acts on the skin surface of the whole body and the affected area. Compared with internal medicine, the curative effect is faster, without any side effects, and will not increase the burden on the liver. It can also improve skin metabolism, promote blood circulation, kill viruses, eliminate bacteria, enhance disease resistance, make skin soft, shiny, elastic, and not easy to relapse after treatment. It has a good preventive effect on healthy dogs.

Detailed ways

The present invention will be described in further detail below according to specific embodiments.

Example 1

A traditional Chinese medicine bath liquid composition for dogs and a preparation method thereof, comprising the following components of Chinese herbal medicine raw materials: sophora flavescens, cicada slough, almond, wormwood, cnidium, hedyotis diffusa, forsythia, mint, aloe, propolis , Artemisia annua, also includes garlic cloves, green salt, lemongrass essential oil. The specific ratio of each component in the raw material of Chinese herbal medicine is shown in Table 1.

A preparation method of a Chinese medicine bath liquid composition for dogs, comprising the following steps:

(1) Raw material processing: Sophora flavescens, almonds, mint, aloe vera, propolis, Artemisia annua, garlic cloves and wormwood were dried at 35°C for 12 hours, crushed and sieved, then added green salt and mixed well to obtain a mixture .

(2) Water leaching: mix the mixture obtained in step (1) with water according to a solid-to-liquid ratio of 1:3 by mass to volume, then heat to 40°C, and leaching for 20 minutes under the assistance of 700W ultrasonic waves. After the end, a single layer of 80-100 mesh gauze was filtered to obtain filtrate I; the filter residue and water were mixed according to the solid-to-liquid ratio of 1:2 according to the mass volume ratio, and ultrasonic-assisted leaching and filtration were carried out in the same way, repeated 2 times , to obtain filtrate II; combine filtrate I and filtrate II to obtain water extract.

(3) Ultrasonic countercurrent extraction: the leaching filter residue obtained in step (2) is mixed with 55% ethanol according to the solid-to-liquid ratio of 1:2 according to the mass volume ratio, and after countercurrent extraction for 50 min under the assisted action of 1000W ultrasonic waves, the monolayer of 80 Filter through mesh gauze to obtain extract I; add equal weight of 55% ethanol to the filter residue to perform ultrasonic-assisted countercurrent extraction and filtration in the same way, repeat once to obtain extract II; combine extract I and extract II to obtain countercurrent extract .

(4) Water decoction extraction: get cicada slough, cnidium fruit, hedyotis diffusa and forsythia plus 5 times the weight of water to soak for 1h, then boil with high fire, turn to slow fire and decoct for 1.5h, filter, and collect decoction 1; Add 2 times the weight of the dregs of water to boil, turn to low heat and decoct for 0.5h, filter and collect decoction II; combine decoction I and decoction II to obtain water decoction.

(5) Mixing and concentration: the water decoction prepared in step (4) is cooled to below 50°C, the water extract prepared in step (2) and the countercurrent extract prepared in step (3) are added and mixed evenly, and then Concentrated by low-temperature evaporation at 40°C to obtain a thick liquid of traditional Chinese medicine.

(6) Disinfection and sterilization: the traditional Chinese medicine viscous liquid prepared in step (5) is sterilized by ultraviolet rays, mixed with lemongrass essential oil, then aseptically bagged or canned to obtain a 300 g finished product.

When using the traditional Chinese medicine bath liquid composition for dogs prepared by this method, the volume ratio of liquid medicine: water (water temperature is not easy to exceed 60°C to prevent inactivation of active ingredients in the medicine and reduce drug efficacy) is 1:100-3000. After mixing (prevention concentration is low, treatment concentration is high), pet dogs can be sprayed or showered. After soaking or showering, it can be washed with water or not. Because of the smell of essential oils, even if you don’t use water to clean the medicinal smell It is also not obvious, and the prevention and treatment effects are more durable.

Example 2

A traditional Chinese medicine bath liquid composition for dogs and a preparation method thereof, comprising the following components of Chinese herbal medicine raw materials: sophora flavescens, cicada slough, almond, wormwood, cnidium, hedyotis diffusa, forsythia, mint, aloe, propolis , Artemisia annua, also includes garlic cloves, green salt, Torreya essential oil. The specific ratio of each component in the raw material of Chinese herbal medicine is shown in Table 1.

A preparation method of a Chinese medicine bath liquid composition for dogs, comprising the following steps:

(1) Raw material processing: Sophora flavescens, almonds, mint, aloe vera, propolis, Artemisia annua, garlic cloves and wormwood were dried at 50°C for 24 hours, crushed and sieved, then added green salt and mixed well to obtain a mixture .

(2) Water leaching: mix the mixture obtained in step (1) with water according to a solid-to-liquid ratio of 1:5 in mass-to-volume ratio, then heat to 50°C, and leaching for 30 minutes under the assistance of 900W ultrasonic waves. After the end, 3 layers of 100 mesh gauze were filtered to obtain filtrate I; the filter residue and water were mixed according to the solid-to-liquid ratio of 1:3 according to the mass-to-volume ratio, and ultrasonic-assisted leaching and filtration were carried out in the same way, repeated 3 times to obtain Filtrate II; combine filtrate I and filtrate II to obtain water extract.

(3) Ultrasonic countercurrent extraction: the leaching filter residue obtained in step (2) is mixed with 55-60% ethanol according to a solid-to-liquid ratio of 1:3 in mass volume ratio, and after countercurrent extraction for 80 minutes under the assisted action of 1200W ultrasonic waves, three layers The 100-mesh gauze was filtered to obtain the extract I; the filter residue was added with 60% ethanol of equal weight to carry out ultrasonic-assisted countercurrent extraction and filtration in the same way, and repeated twice to obtain the extract II; the combined extract I and extract II were obtained countercurrent extract.

(4) Water decoction extraction: get cicada slough, cnidium fruit, hedyotis diffusa and forsythia plus 8 times the weight of water soak for 2h, then boil with high fire, turn to slow fire and decoct for 2h, filter, collect decoction 1; medicine Add 4 times the weight of water to the slag and boil over high heat, turn to low heat and decoct for 1 hour, filter, and collect decoction II; combine decoction I and decoction II to obtain water decoction.

(5) Mixing and concentration: the water decoction prepared in step (4) is cooled to below 50°C, the water extract prepared in step (2) and the countercurrent extract prepared in step (3) are added and mixed evenly, and then Concentrated by low-temperature evaporation at 45°C to obtain a thick liquid of traditional Chinese medicine.

(6) Disinfection and sterilization: the traditional Chinese medicine viscous liquid prepared in step (5) is sterilized with 20KGy60Co, added Torreya torreya essential oil and mixed, then aseptically bagged or canned to obtain 300g finished product.

When using the traditional Chinese medicine bath liquid composition for dogs prepared by this method, the volume ratio of liquid medicine: water (water temperature is not easy to exceed 60°C to prevent inactivation of active ingredients in the medicine and reduce drug efficacy) is 1:300-2000. (Prevention concentration is low, treatment concentration is high) After mixing, it can be sprayed or showered for pet dogs. After soaking or showering, it can be washed with water or not. Because of the smell of essential oils, even if you don’t use water to wash off the medicinal smell It is also not obvious, and the prevention and treatment effects are more durable.

Example 3

A traditional Chinese medicine bath liquid composition for dogs and a preparation method thereof, comprising the following components of Chinese herbal medicine raw materials: sophora flavescens, cicada slough, almond, wormwood, cnidium, hedyotis diffusa, forsythia, mint, aloe, propolis , Artemisia annua, also includes garlic cloves, green salt, tea tree essential oil. The specific ratio of each component in the raw material of Chinese herbal medicine is shown in Table 1.

A preparation method of a Chinese medicine bath liquid composition for dogs, comprising the following steps:

(1) Raw material processing: Sophora flavescens, almonds, mint, aloe vera, propolis, Artemisia annua, garlic cloves and wormwood were dried at 40°C for 16 hours, crushed and sieved, then added green salt and mixed well to obtain a mixture .

(2) Water leaching: mix the mixture obtained in step (1) with water according to a solid-to-liquid ratio of 1:4 in mass-to-volume ratio, then heat to 45°C, and leaching for 25 minutes under the assisted action of 800W ultrasonic waves. After the end, 2 layers of 80-100 mesh gauze were filtered to obtain filtrate I; the filter residue and water were mixed according to the solid-to-liquid ratio of 1:2.5 according to the mass volume ratio, and ultrasonic-assisted extraction and filtration were carried out in the same way, repeated 2 times , to obtain filtrate II; combine filtrate I and filtrate II to obtain water extract.

(3) Ultrasonic countercurrent extraction: the leaching filter residue obtained in step (2) is mixed with 58% ethanol according to the solid-to-liquid ratio of 1:2.5 according to the mass volume ratio, and after countercurrent extraction for 60min under the assisted action of 1100W ultrasonic waves, the 90 Filter through mesh gauze to obtain extract I; add equal weight of 58% ethanol to the filter residue to perform ultrasonic-assisted countercurrent extraction and filtration in the same way, repeat once to obtain extract II; combine extract I and extract II to obtain countercurrent extract .

(4) Water decoction extraction: take cicada slough, cnidium fruit, hedyotis diffusa and forsythia plus 6 times the weight of water and soak for 1.5h, then boil with high fire, turn to slow fire and decoct for 1.8h, filter, and collect the decoction 1 Add 3 times the weight of the dregs of water to boil, turn to low heat and decoct for 0.5h, filter, and collect the decoction II; combine the decoction I and decoction II to obtain the water decoction.

(5) Mixing and concentration: the water decoction prepared in step (4) is cooled to below 50°C, the water extract prepared in step (2) and the countercurrent extract prepared in step (3) are added and mixed evenly, and then Concentrated by low-temperature evaporation at 40°C to obtain a thick liquid of traditional Chinese medicine.

(6) Disinfection and sterilization: the traditional Chinese medicine thick liquid prepared in step (5) is sterilized by ultraviolet rays, added tea tree essential oil and mixed, then aseptically bagged or canned to obtain a 300g finished product.

When using the traditional Chinese medicine bath liquid composition for dogs prepared by the method, the volume ratio of liquid medicine: water (water temperature is not easy to exceed 60°C to prevent inactivation of active ingredients in the medicine and reduce drug efficacy) is 1:500-1000. (Prevention concentration is low, treatment concentration is high) After mixing, it can be sprayed or showered for pet dogs. After soaking or showering, it can be washed with water or not. Because of the smell of essential oils, even if you don’t use water to wash off the medicinal smell It is also not obvious, and the prevention and treatment effects are more durable.

Example 4

A traditional Chinese medicine bath liquid composition for dogs and a preparation method thereof, comprising the following components of Chinese herbal medicine raw materials: sophora flavescens, cicada slough, almond, wormwood, cnidium, hedyotis diffusa, forsythia, mint, aloe, propolis , Artemisia annua, also includes garlic cloves, green salt, lemongrass essential oil, torreya essential oil, tea tree essential oil. The specific ratio of each component in the raw materials of Chinese herbal medicine is shown in Table 1.

A preparation method of a Chinese medicine bath liquid composition for dogs, comprising the following steps:

(1) Raw material processing: Sophora flavescens, almonds, mint, aloe vera, propolis, Artemisia annua, garlic cloves and wormwood were dried at 45°C for 18 hours, crushed and sieved, then added green salt and mixed well to obtain a mixture .

(2) Water leaching: mix the mixture obtained in step (1) with water according to a solid-to-liquid ratio of 1:4.5 in mass-to-volume ratio, then heat to 45°C, and leaching for 28 minutes under the assistance of 850W ultrasonic waves. After the end, 2 layers of 90-mesh gauze were filtered to obtain filtrate I; the filter residue and water were mixed according to the solid-to-liquid ratio of 1:3 according to the mass-to-volume ratio, and ultrasonic-assisted leaching and filtration were carried out in the same way, repeated 3 times to obtain Filtrate II; combine filtrate I and filtrate II to obtain water extract.

(3) Ultrasonic countercurrent extraction: the leaching filter residue obtained in step (2) is mixed with 55% ethanol according to the solid-to-liquid ratio of 1:2 according to the mass volume ratio, and after countercurrent extraction for 70 minutes under the assisted action of 1000W ultrasonic waves, the 2-layer 80 Filter through mesh gauze to obtain extract I; add equal weight of 60% ethanol to the filter residue to carry out ultrasonic-assisted countercurrent extraction and filter in the same way, repeat twice to obtain extract II; combine extract I and extract II to obtain countercurrent extract .

(4) Water decoction extraction: get cicada slough, Cnidium fruit, Hedyotis diffusa and Forsythia, add 7 times the weight of water to soak for 2 hours, then boil with high heat, turn to simmer for 5-2 hours, filter, and collect decoction 1 Add 4 times the weight of the dregs of water to boil, turn to low heat and decoct for 1 hour, filter, and collect the decoction II; combine the decoction I and decoction II to obtain the decoction.

(5) Mixing and concentration: the water decoction prepared in step (4) is cooled to below 50°C, the water extract prepared in step (2) and the countercurrent extract prepared in step (3) are added and mixed evenly, and then Concentrated by low-temperature evaporation at 45°C to obtain a thick liquid of traditional Chinese medicine.

(6) Disinfection and sterilization: the traditional Chinese medicine viscous liquid prepared in step (5) is sterilized by ultraviolet rays, mixed with lemongrass essential oil, Chinese torreya essential oil, and tea tree essential oil, and then aseptically bagged or canned to obtain 300 g of finished product.

When using the traditional Chinese medicine bath liquid composition for dogs prepared by this method, the volume ratio of liquid medicine: water (water temperature is not easy to exceed 60°C to prevent inactivation of active ingredients in the medicine and reduce drug efficacy) is 1:100-500. (Prevention concentration is low, treatment concentration is high) After mixing, it can be spray bathed or showered for pet dogs. After soaking or showering, it can be washed with water or not, because the smell of essential oil is covered, even if the smell of medicine is not washed with water It is also not obvious, and the prevention and treatment effects are more durable.

Table 1. The composition ratio of herbal medicine raw materials in each embodiment

Example 5

1. The in vitro fungal inhibition test of the traditional Chinese medicine bath liquid composition for dogs

The Chinese medicine bath liquid composition for dogs prepared by the embodiments of the present invention 1-4 (diluted into different concentration gradients with double distilled water respectively) is the experimental group; the double distilled water is used as the blank control group, and the commercially available clotrimazole (purchased from Shanghai Binzhi Biotechnology Co., Ltd., diluted with double distilled water into different concentration gradients) was used as a positive control group. In vitro fungal inhibition experiment: Autoclave Sabouraud medium at 121°C for 20 minutes, after cooling, take 1ml of the diluted drug from each control group and experimental group for coating, and inoculate at 28-30°C for 3 days, observations were made, and the results are shown in Table 2. As can be seen from Table 2, the antifungal effect of embodiment 4 is the best, thus it can be seen that after a certain proportioning mixed essential oil is added, not only the smell in the original Chinese medicine can be covered, but also a certain bactericidal effect can be played.

Table 2. The antifungal activity of the Chinese medicine bath composition for dogs of each embodiment

In Table 2, MIC represents the minimum inhibitory concentration, and MSC represents the minimum bactericidal concentration (the original concentration before dilution is 1).

2. In vitro bacterial inhibition test of the traditional Chinese medicine bath composition for dogs

The Chinese medicine bath liquid composition for dogs prepared by the embodiments of the present invention 1-4 (diluted into different concentration gradients with double distilled water respectively) was used as the experimental group; the double distilled water was used as the blank control group, and commercially available penicillin (purchased Zishenggong Bioengineering (Shanghai) Co., Ltd., diluted with double distilled water into different concentration gradients) was used as a positive control group. Carry out in vitro bacterial inhibition experiment: Autoclave LB medium at 121°C for 20 minutes, after cooling, take 1ml of the diluted drug from each control group and experimental group for coating, incubate at 37°C for 24 days after inoculation, and carry out Observation, the results are shown in Table 3. As can be seen from Table 3, the fungal inhibitory effect of embodiment 4 is the best, thus it can be seen that after a certain proportioning mixed essential oil is added, not only the smell in the former Chinese medicine can be covered, but also a certain bactericidal effect can be played.

Table 3. The antibacterial activity of each embodiment dog Chinese medicine bath composition

In Table 3, MIC represents the minimum inhibitory concentration, and MSC represents the minimum bactericidal concentration (the original concentration before dilution is 1).

3. Inhibitory test of the traditional Chinese medicine bath liquid composition for dogs on mites

3.1 Experimental grouping: Chinese medicine bath composition for dogs prepared according to Example 4 of the present invention (diluted to 100%, 50%, 25%, 10%, 1%, 0.1%, 0.01% concentration with double distilled water respectively) Be the experimental group; use double distilled water as the blank control group, and set 2% metronidazole solution as the positive control group as the positive control group. There are 10 Demodex mites in every group, and the experiment is repeated 3 times for each medicinal solution concentration, and the results are averaged.

3.2 Observation method: Use a micropipette to absorb 500 μL of the drug solution and drop it on the slide, spread the drug solution evenly with a pusher, and stick the tape on which Demodex was detected on the glass slide to ensure that the drug solution and the insect body are fully touch. Then the slides were placed in an artificial climate chamber (30°C in temperature, 75% in humidity), and the death of Demodex was observed under a microscope after 12 hours.

3.3 Judgment criteria for death: Continuous observation under a microscope at 400 times for 1 minute, the mite body chelicerae or feet that do not move are preliminarily judged as dead, and those who continue to observe for 30 minutes and still do not move are diagnosed as dead.

Corrected mortality rate/%=(the number of surviving mites in the blank control group - the number of surviving mites in the treatment group)/the number of surviving mites in the blank control group×100

3.4 Results

Table 4. The corrected mortality rate of the Chinese medicinal bath liquid composition for dogs of different concentrations of embodiment 4

group Corrected mortality rate (%)±SE 100% concentration 100 50% concentration 100 25% concentration 96±6.3 10% concentration 80±9.1 1% concentration 57±5.7 0.1% concentration 39±2.3 0.01% concentration 11±1.8 Blank control group (double distilled water) 0 Positive control group (2% metronidazole solution) 62.7±2.5

As can be seen from Table 4, the traditional Chinese medicine bath liquid composition for dogs has a significant killing effect on mites; its killing effect increases with the increase of concentration, and when the concentration reaches 50%, the mite lethality rate is already 100%; When the solution concentration is 25%, the mite lethality rate is about 96%; when the solution concentration is 10%, the mite lethality rate is about 80%; when the solution concentration is 1%, the mite lethality rate is about 57%; when the solution When the concentration is 0.1%, the mite lethality rate is about 39%. Experiments have proved that the traditional Chinese medicine bath liquid composition for dogs has a killing effect on Demodex mites and the effect is extremely significant.

4. Mouse skin irritation test

Animal grouping and pre-experiment treatment: Take 20 healthy white rabbits, weighing 25-30g, half male and half female, feed them for 1 week before the experiment, and observe diet and behavior. 4 in each group. 24 hours before the administration, the hair on both sides of the back and spine of the mice in each group was subtracted, and the area of the hair removal area on each side was 1 cm ² . Administration method: the Chinese medicine bath liquid composition for dogs of Example 4 is diluted with clear water 0 times (stock solution), 10 times, 100 times, 1000 times, 10000 times respectively, and is applied to the left depilatory area of each group of mice respectively , smeared 5 times, each dosage 1ml; the right depilatory area of the mouse was coated with the same amount of clear water; After keeping the drug in contact with the skin for 6 hours, remove the gauze and plastic wrap, and then wash off the residual drug with warm water (the hair removal area on the right side is also washed with warm water); administer once a day for 7 consecutive days. Remove the drug for 2 hours each time and observe the skin condition before re-administration. Observe again on 1, 12, 24, and 48 after the last administration and removal of the drug, and record whether there is erythema, edema, and recovery at the smeared site. The evaluation criteria are shown in Table 5 and Table 6.

Table 5. Criteria for Skin Irritation Symptoms

Table 6. Evaluation of skin irritation intensity

Score Evaluation 0-0.5 non-irritating 0.5-2 mild irritant 2-6 Toxic irritation 6-8 Intensity stimulus

By experimental administration and visual observation, it can be seen that the skin test of the right depilatory area has no obvious erythema, edema and other skin irritation symptoms in the whole process; The 10 times (i.e. 10% concentration) diluted solution had mild erythema and edema 1h after removing the drug and 1h after the last administration and 1h after removing the drug, which were barely visible, but 6h after removing the drug and the last administration after the last administration The erythema and edema disappeared 12 hours after the drug and the drug was removed; the diluted solution diluted 100 times (ie 1% concentration) and above multiple times was applied to the left hair removal area, 1 hour after the drug was removed every day, before the re-administration and the last time After the last administration and 1, 12, 24, and 48 hours after drug removal, there were no obvious skin irritation symptoms such as erythema and edema, and all scores were zero. It can be seen that it is safe to shower or spray bath pet dogs when the stock solution of the traditional Chinese medicine bath liquid composition for dogs of the present invention is diluted 100 times or more (that is, the concentration is lower than 1%).

5. Canine treatment effect

In a pet hospital, I treated canine infectious skin diseases caused by bacteria, fungi and parasites. The skin of the sick dog's face, neck, back and abdomen was flushed and itchy, and hair loss occurred in different degrees at the infected area. There are pustules in the affected area, the skin is thickened, and the skin is moist and bright red after removing the scab. The ear tip and tail root of the sick dog are covered with a large number of scabs and scales, the hair on the head, face and other parts of the body surface is broken, and there are more dander in the circular depilatory area.

The sick dogs were diluted 100 times (ie 1% concentration of the stock solution) with the medicine provided by the embodiment of the present invention 1-4 as the experimental group; the blank control group was not suitable for drug treatment, and only soaked in clear water. The specific operation is: soak the dog for 30 minutes (the water temperature is controlled at 35-40°C), soak once every 2 days, and use it for 10 consecutive days. The effect is remarkable.

Table 7. Judgment criteria for the cure of canine skin diseases

Table 8. Therapeutic effects of different drugs on different canine skin diseases

As can be seen from Table 7 and Table 8, the application of the 1% concentration of the Chinese medicine bath liquid composition for dogs of the embodiments of the present invention 1-4, the cure rate is more than 80%, and the total effective rate is more than 90%. It can be seen from this that The traditional Chinese medicine bath liquid composition for dogs of the invention has good effect on treating canine contagious skin diseases caused by bacteria, fungi and parasites.

Although the present invention has been described in detail with general description and specific examples above, it will be obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made on the basis of not departing from the spirit of the present invention all belong to the protection scope of the present invention.

Claims (10)

1. a kind of dog Chinese medicine bath lotion composition and preparation method thereof, it is characterised in that：Chinese herbal medicine including following parts by weight is former Each component in material：10-15 parts of kuh-seng, 3-5 parts of cicada slough, 6-10 parts of almond, 20-30 parts of wormwood, 10-15 parts of frutus cnidii, white flower 5-8 parts of HERBA HEDYOTIS DIFFUSAE, 5-6 parts of Fructus Forsythiae, 10-15 parts of peppermint, 10-15 parts of aloe, 3-5 parts of propolis.

2. dog according to claim 1 Chinese medicine bath lotion composition, it is characterised in that：Further include 6-8 parts of artemisia annua.

3. dog according to claim 2 Chinese medicine bath lotion composition, it is characterised in that：Further include 10-13 parts of garlic garlic clove.

4. dog according to claim 3 Chinese medicine bath lotion composition, it is characterised in that：Further include 2-3 parts of green salt.

5. dog according to claim 4 Chinese medicine bath lotion composition, it is characterised in that：Further include 0.5-2 parts of essential oil；It is described Essential oil is lemon grass (Cymbopogon citratus) essential oil, Chinese torreya essential oil, the one or more of tea tree ethereal oil.

6. dog according to claim 5 Chinese medicine bath lotion composition, it is characterised in that：The essential oil for lemon grass (Cymbopogon citratus) essential oil, Chinese torreya essential oil, tea tree ethereal oil are according to weight ratio 1:2:1.5 it mixes.

7. according to the preparation method of claim 4-6 any one of them dog Chinese medicine bath lotion composition, which is characterized in that including Following steps：

(1) feedstock processing：Kuh-seng, almond, peppermint, aloe, propolis, artemisia annua, garlic garlic clove and wormwood are dried respectively Dry, then grinding and sieving adds in abundant mixing after green salt, obtains mixture；

(2) flooding：It heats after mixture obtained by step (1) is added water, is extracted under ultrasonic wave booster action, extraction knot Shu Hou, filter to get filtrate I；Filter residue adds water to carry out Ultrasonic Wave-Assisted Extraction, filtering after the same method, repeats 2-3 times, must filter Liquid II；Merging filtrate I, filtrate II are up to water extract；

(3) ultrasound adverse current is extracted：55-60% ethyl alcohol is added in into the extraction filter residue obtained by step (2), under ultrasonic wave booster action Adverse current is extracted, and filters to obtain extracting solution I；Filter residue such as adds to carry out ultrasonic wave auxiliary after the same method at the 55-60% ethyl alcohol of weight Adverse current extraction, filtering, repeat 1-2 times, obtain extracting solution II；Merge extracting solution I, extracting solution II up to adverse current extracting solution；

(4) water boiling and extraction：Cicada slough, frutus cnidii, oldenlandia diffusa and Fructus Forsythiae are taken, the water of 5-8 times of weight is added to impregnate 1-2h, so Intense fire is boiled afterwards, is turned after being cooked by slow fire 1.5-2h, and decoction liquor I is collected in filtering；The dregs of a decoction add the water intense fire of 2-4 times of weight to boil, and turn text After the pan-fried 0.5-1h of fire, decoction liquor II is collected in filtering；Merge decoction liquor I, decoction liquor II up to water decoction；

(5) mix, concentrate：It treats that water decoction prepared by step (4) is cooled to less than 50 DEG C, adds in water logging prepared by step (2) Adverse current extracting solution prepared by extract and step (3) is uniformly mixed, and then low-temperature evaporation is concentrated to give Chinese medicine magma；

(6) sterilization：Chinese medicine magma sterilization prepared by step (5), after adding in essential oil mixing, through sterile pack or tank It is got product after dress.

8. the dog according to claim 7 preparation method of Chinese medicine bath lotion composition, it is characterised in that：In step (1), dry Dry temperature is 35-50 DEG C, drying time 12-24h；Smash it through 60 mesh sieves；In step (2), the mixture and water according to Mass volume ratio is 1:The solid-to-liquid ratio mixing of 3-5, is then heated to 40-50 DEG C, under 700-900W ultrasonic wave booster actions, leaching 20-30min is carried, after extraction, 1-3 layers of 80-100 mesh filtered through gauze obtains filtrate I；The filter residue is with water according to quality volume Than for 1:The solid-to-liquid ratio mixing of 2-3.

9. the dog according to claim 7 preparation method of Chinese medicine bath lotion composition, it is characterised in that：In step (3), institute It is 1 according to mass volume ratio that extraction filter residue, which is stated, with 55-60% ethyl alcohol:The solid-to-liquid ratio mixing of 2-3, it is auxiliary in 1000-1200W ultrasonic waves After helping the lower adverse current extraction 50-80min of effect, 1-3 layers of 80-100 mesh filtered through gauze obtains extracting solution I.

10. the dog according to claim 7 preparation method of Chinese medicine bath lotion composition, it is characterised in that：In step (5), The temperature of low-temperature evaporation is 40-45 DEG C；In step (6), the sterilization mode is ultraviolet-ray sterilizing or 20KGy60Co One kind in sterilization.