CN107304406A - A kind of microorganism formulation preparation method for making crop material rotten - Google Patents
A kind of microorganism formulation preparation method for making crop material rotten Download PDFInfo
- Publication number
- CN107304406A CN107304406A CN201610241526.8A CN201610241526A CN107304406A CN 107304406 A CN107304406 A CN 107304406A CN 201610241526 A CN201610241526 A CN 201610241526A CN 107304406 A CN107304406 A CN 107304406A
- Authority
- CN
- China
- Prior art keywords
- strains
- floras
- filter mud
- microorganism formulation
- crop material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The present invention relates to a kind of microorganism formulation preparation method for making crop material rotten, microorganism used therefor flora is named as WH floras, it is bacillus, using three-stage culture medium and carrier of the filter mud as WH floras, stalk rotting agent is made by processing steps such as the preparation of WH floras, second class inoculum culture, three-class strain cultures.The catabolite of WH floras provides energy and nutrition to indigenous cellulose-decomposing bacteria and various corrupt floras, their acute activations is cooperateed with together with WH floras reciprocally to crop material fast rotting.This law technique is simple, cost is low, containing viable count it is high, easy to use, be easy to be widely applied.
Description
Technical field
The present invention relates to microorganism formulation, more particularly to a kind of microorganism formulation preparation side for making straw rotten
Method.
Background technology
China often produces agricultural crop straw per year up to several hundred million tons, except on a small quantity directly as feed in addition to, a large amount of remaining crop materials
It is burned off, pollutes environment.Scientific worker is directed to the corruption of crop material heap is returned into field using microorganism for many years.Have one at present
Based on kind thermophilic sporotrichum a kind of microorganism formulation is synthesized with other several high-effective microorganisms --- rotten bacillum.It is this
Stalk rotting agent prepares more complicated, first has to separately individually cultivate multiple-microorganism is included, can not pollute mutually.In building-up process,
Such as operating not enough will carefully set off an explosion, and jeopardize personal safety, and used chemical addition agent consumption is improper, can also influence
Microbial survival rate.
The content of the invention
It is an object of the invention to provide a kind of microorganism species very capable to crop material decomposition, micro- life is utilized
Thing flora goes consumingly to decompose crop stalk fibre, so that crop material fast rotting is into fertilizer.
The object of the present invention is achieved like this：A kind of microorganism formulation for making crop material rotten, its Main Ingredients and Appearance is
WH1 strains, WH2 strains, WH3 strains, WH4 strains, WH5 strains and the extract that sucrose is produced with sulfurous method --- filter
Mud and rice bran.
The proportioning of strain contained by mentioned microorganism preparation is (percentage by weight)：
(1) WH1 strains：5-20%
(2) WH2 strains：17-35%
(3) WH3 strains：10-30%
(4) WH4 strains：8-15%
(5) WH5 strains：25-35%.
The preparation method of mentioned microorganism preparation, including Spawn incubation and stack retting, its processing step are as follows：
(1) by WH1 strains, WH2 strains, WH3 strains, WH4 strains, WH5 strains are well mixed by said ratio, constitute WH bacterium
Group；
(2) second class inoculum culture medium prescription (percentage by weight) is：
The meat meal tankage 0.1% of 80% soy meal of rice bran, 16% milk powder 2%
Sugared 0.8% water 1%. of fishbone dust 0.1%.
(3) second class inoculum culture：First second class inoculum culture medium is mixed by above-mentioned formula and mixed thoroughly, WH is inoculated with normal temperatures
Flora, cultivation temperature is 35 DEG C -40 DEG C, pH value 7.0, and incubation time 7-9 days obtains the second order activity chaff bacterium of WH floras；
(4) three-class strain culture：Use using sulfurous method produce sucrose extract --- filter mud is used as three-class strain culture medium
And its carrier, filter mud Main Ingredients and Appearance and its content average value are (percentage by weight)：
Organic matter：58.66% N：1.19%
P2O5：1.24% K2O：0.52%
CaO：5.59% MgO：1.07%
PH value： 6.0.
The fermentation process of WH floras：Filter mud is inoculated with two grades of WH floras when temperature is 20 DEG C -50 DEG C, and inoculum concentration is 0.01-
1%, stack retting is layered after inoculation, vaccinated filter mud temperature gradually rises up to 60 DEG C -65 DEG C, pH value 6.8- during fermentation
7.2, through 7-12 days fermenting-ripenings.Specific operation process is such：Two grades of WH floras after dilution are uniformly sprayed at filter mud
Go up and be deposited on the vacant lot of ceiling, prevent from exposing to the sun and rain, influence ferment effect.Stack specification：It is high 8-10 centimetres, length and width
Depending on landform, black thin film in upper cover makes have enough temperature during filter mud fermentation, and can be sun-proof, dirty to reduce air
Dye.Film was opened sooner or later once daily from second day during stack retting, and 5 minutes every time, covering film again afterwards made fermentation
When have enough oxygen.There is within 3rd day white hypha, cover with white hypha within the 5th day, afterwards, can not lid film,
Seven-ten two days filter mud fermentation maturations.Filter mud fermentation turns into microorganism into rear viable count up to 1,000,000,000-1,000 hundred million/gram after drying
The base-material of preparation --- stalk rotting agent.
(5) by the second order activity chaff bacterium of base-material and WH floras according to the following ratio (percentage by weight) mix, crush, packaging
Into stalk rotting agent：
Base-material：80-90%
The second order activity chaff bacterium of WH floras：10-20%.
The present invention is in the filter mud containing the element such as abundant organic matter and N, P, K, Ca, Mg using WH floras open-architecture controller
On, filter mud is both culture medium and carrier, the rapid flourish in the carrier of WH floras under suitable temperature and humidity, its
Metabolite improves environment, the product of degraded also to indigenous cellulose-decomposing bacteria and various corrupt floras provide energy and
Nutrition, makes their acute activations be cooperateed with together with WH floras reciprocally to crop material fast rotting into fertilizer.
Compared with existing crop material rots technology, the present invention has the positive effect that：
1st, extract --- the microbial bacteria used in filter mud as stalk rotting agent of the present invention that sucrose is produced using sulfurous method is used
The three-stage culture medium and carrier of group, solves " three wastes " problem in sugar refinery, is conducive to production, is conducive to environmental protection.
2nd, simple production process, cost are low, short containing viable count height, heap rotten time.
3rd, rotten stalk mode both can with stack retting, can also crop field depression bar, method is simple, effect is good, it is easy to which being widely applied should
With.
The present invention is further elaborated by example below.
Embodiment 1：
1st, the microorganism formulation for making crop material rotten.
(1) WH1 strains：5% (2) WH2 strains：35%
(3) WH3 strains：10% (4) WH4 strains：15%
(5) WH5 strains：35%.
2nd, the preparation method of microorganism formulation --- stalk rotting agent：
(1) WH bacterium are prepared：WH1 strains, WH2 strains, WH3 strains, WH4 strains, WH5 strains are well mixed by said ratio,
Constitute WH floras.
(2) second class inoculum culture：
Second class inoculum culture mixes preparation (percentage by weight) thoroughly by following formula mixing：
The meat meal tankage 0.1% of 80% soy meal of rice bran, 16% milk powder 2%
Sugared 0.8% water 1% of fishbone dust 0.1%.
The second class inoculum culture medium prepared is inoculated with WH floras at normal temperatures, cultivation temperature is 35 DEG C, PH values 7.0, training
9 days time is supported, the second order activity chaff bacterium of WH floras is obtained.
(3) three-class strain culture：Use using sulfurous method produce sucrose extract --- filter mud is trained as three-class strain
Base and its carrier are supported, filter mud Main Ingredients and Appearance and its content average value are (percentage by weight)：
Organic matter：58.6 6% N：1.19%
P2O51.24% K2O：0.52%
CaO：5.59% MgO：1.07%
PH value： 6.0.
The fermentation process of WH floras：Filter mud is inoculated with two grades of WH floras when temperature is 20 DEG C, and inoculum concentration is 1%, after inoculation
Stack retting is layered, vaccinated filter mud temperature gradually rose up to 60 DEG C during fermentation, pH value 6.8, by 12 days fermenting-ripenings,
Turn into the base-material of stalk rotting agent after drying, the base-material contains viable count up to 1,000,000,000-1,000 hundred million/gram.Specific operation process is so
's：Two grades of WH floras after being diluted with water by above-mentioned inoculum concentration are uniformly sprayed on filter mud and are deposited in the vacant lot of ceiling
On, prevent from exposing to the sun and rain, influence ferment effect.Stack specification：High 8-10 centimetres, length and width is black in upper cover depending on landform
Color film, makes have enough temperature during filter mud fermentation, and can be sun-proof, to reduce air pollution.From second day during stack retting
Rise and open film sooner or later once daily, 5 minutes every time, cover film again afterwards, make have enough oxygen during fermentation.3rd day
There is white hypha, cover with white hypha within the 5th day, afterwards, can not lid film, the 12nd day filter mud fermentation maturation.
90% base-material and 10% WH flora second order activity chaff bacterium (by weight percentage) are mixed, crushed, packaging is made
Into stalk rotting agent.
Embodiment 2：
The present embodiment is with the difference of example 1：
1st, the proportioning of WH floras is (percentage by weight)：
(1) WH1 strains：12% (2) WH2 strains：27% (3) WH3 strains：20%
(4) WH4 strains：11% (5) WH5 strains：30%
2nd, second class inoculum condition of culture：Cultivation temperature is 37 DEG C, incubation time 8 days.
3rd, the fermentation condition of WH floras：Filter mud is inoculated with WH floras at 35 DEG C, and inoculum concentration is 0.1%.Inoculation fermentation
Filter mud temperature gradually rises up to 62 DEG C, pH value 7.0, through 10 days fermenting-ripenings.
Remaining parameter and processing step are same as Example 1.
Finally, 85% base-material and 15% WH flora second order activity chaff bacterium (by weight percentage) are mixed, crushed,
Stalk rotting agent is made in packaging.
Embodiment 3：
The present embodiment is with the difference of example 1：
1st, the proportioning of WH floras is (percentage by weight)：
(1) WH1 strains：20% (2) WH2 strains：17% (3) WH3 strains：30%
(4) WH4 strains：8% (5) WG5 strains：25%
2nd, second class inoculum condition of culture：Cultivation temperature is 40 DEG C, incubation time 7 days.
3rd, the fermentation condition of WH floras：Filter mud is inoculated with WH floras at 50 DEG C, and inoculum concentration is 0.01%, inoculation fermentation
Filter mud temperature gradually rise up to 65 DEG C, pH value 7.2, through 7 days fermenting-ripenings.
Remaining parameter and processing step are same as Example 1.
Finally 80% base-material and 20% WH flora second order activity chaff bacterium (by weight percentage) are mixed, crush, wrapped
Stalk rotting agent is made in dress.
Embodiment 4：
Rice straw stack retting example：First press per 100Kg fresh rice straws, with stalk rotting agent amount 0.3Kg, that urea 1kg is watered 40Kg is standby into mixed liquor
With.Fresh rice straw is kept flat on the ground, it puts specification for width 1.5m × high 0.15m × long 2m, above-mentioned stalk rotting agent mixed liquor
Pouring is equably spilt on fresh rice straw, the second layer, third layer are then put again, one up to the tenth layer, and every layer is spilt pouring stalk rotting agent and mixed
Close liquid.Every ten layers are a pile, and top is sealed with mud or covered with black thin film.Through 1-2 weeks, fresh rice straw color was by light yellow
Become taupe, there is odor, the soft corruption of rice straw, you can make base stock fertilizer and apply the cultivated rake of Tanaka.
Embodiment 5：
Cornstalk stack retting example：First by the fresh cornstalk stalk rotting agent amount 0.4Kg of 100Kg, urea 1Kg, be watered 50Kg into mixed liquor
It is standby.Fresh cornstalk is kept flat on the ground, it puts specification for width 1.5m × high 0.15m × long 2m, and above-mentioned stalk rotting agent is mixed
Closing liquid, equably sprinkle puts the second layer, third layer one until the tenth layer, every layer of equal sprinkle again on fresh cornstalk, afterwards
Stalk rotting agent mixed liquor.Black thin film or mud is coated with every ten layers for a pile, in top cover, through 20-30 days, cornstalk color
Become blackyellow, the soft corruption of feel, you can be manured into soil and be used as fertilizer by light yellow.
Embodiment 6：
Crop field depression bar example：Rice straw is uniformly removed in field after early rice harvesting, it is 9- 10cm to discharge water into water in field depth.With reference to tractor-ploughing
1Kg/ mus of 2-3Kg/ mus of stalk rotting agent and urea are uniformly applied when pressing stalk, stalk is pressed 7-10 days, stopping irrigation and drainage entering field during this, macerate stalk
Rice straw all softens and tentatively rots that base manure can be used as after 10 days.
Claims (2)
1. a kind of microorganism formulation preparation method for making crop material rotten, it is characterised in that：The microorganism formulation it is main into
Part is WH1 strains, WH2 strains, WH3 strains, WH4 strains, WH5 strains and the extraction that sucrose is produced with sulfurous method
Thing --- filter mud and rice bran；The proportioning of strain is contained by the microorganism formulation：(1) WH1 strains：5-20% (2) WH2 strains：
17-35% (3) WH3 strains：10-30% (4) WH4 strains：8-15% (5) WH5 strains：25-35%.
2. a kind of microorganism formulation preparation method for making crop material rotten, including Spawn incubation and stack retting, it is characterised in that：
(1) the WH1 strains described in claim 1, WH2 strains, WH3 strains, WH4 strains, WH5 strains are uniformly mixed according to a ratio, group
Into WH floras；(2) second class inoculum culture medium prescription：Rice bran：80% soy meal：16% milk powder：2% meat meal tankage：0.1% fish
Bone meal：0.1% sugar：0.8% water：1%；(3) second class inoculum culture：First second class inoculum culture medium is mixed by above-mentioned formula
Mix thoroughly, WH floras are inoculated with normal temperature, 35 DEG C -40 DEG C of culture medium temperature, pH value 7.0, incubation time 7-9 days obtains WH floras
Second order activity chaff bacterium；(4) three-class strain culture：Use using sulfurous method produce sucrose extract --- filter mud is used as three
Level bacterium culture medium and its carrier, filter mud Main Ingredients and Appearance and its content average value are：Organic matter：58.66% N：1.19%
P2O5：1.24% K2O：0.52% CaO：5.59% MgO：1.07% pH value：6.0；The fermentation process of WH floras：Filter mud
WH floras are inoculated with when temperature is 20-50 DEG C, inoculum concentration is to be layered stack retting after 0.01-1%, inoculation, has been inoculated with during fermentation
Filter mud temperature gradually rise up to 60-65 DEG C, pH value 6.8-7.2 turns into microorganism formulation --- the base of rotten bacillum after drying
Material；(5) the second order activity chaff bacterium of above-mentioned base-material and WH floras mixed according to the following ratio, crush, be packaged into stalk rotting agent：Base
Material：The second order activity chaff bacterium of 80-90% WH floras：10-20%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610241526.8A CN107304406A (en) | 2016-04-19 | 2016-04-19 | A kind of microorganism formulation preparation method for making crop material rotten |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610241526.8A CN107304406A (en) | 2016-04-19 | 2016-04-19 | A kind of microorganism formulation preparation method for making crop material rotten |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107304406A true CN107304406A (en) | 2017-10-31 |
Family
ID=60151258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610241526.8A Pending CN107304406A (en) | 2016-04-19 | 2016-04-19 | A kind of microorganism formulation preparation method for making crop material rotten |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107304406A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108017428A (en) * | 2017-12-12 | 2018-05-11 | 长春工业大学 | A kind of straw decomposition microbial inoculum and its application |
| CN111302853A (en) * | 2020-02-16 | 2020-06-19 | 金葵子环保生物科技(国际)有限公司 | Microbial phosphorus-potassium fertilizer and preparation method thereof |
| CN111763120A (en) * | 2020-06-29 | 2020-10-13 | 赵国良 | Long-acting biological fertilizer |
-
2016
- 2016-04-19 CN CN201610241526.8A patent/CN107304406A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108017428A (en) * | 2017-12-12 | 2018-05-11 | 长春工业大学 | A kind of straw decomposition microbial inoculum and its application |
| CN111302853A (en) * | 2020-02-16 | 2020-06-19 | 金葵子环保生物科技(国际)有限公司 | Microbial phosphorus-potassium fertilizer and preparation method thereof |
| CN111763120A (en) * | 2020-06-29 | 2020-10-13 | 赵国良 | Long-acting biological fertilizer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104774054B (en) | Biological organic fertilizer | |
| CN102531766B (en) | Microbial decomposing agent and production method thereof | |
| CN103966150B (en) | A kind of agricultural crop straw decomposing microbial inoculum and using method thereof and effect | |
| CN101723758B (en) | Method for culturing stropharia rugoso-annulata and compost thereof | |
| CN101514120B (en) | Fermentation fertilizer containing active ingredient of bamboo and method of producing the same | |
| CN101692771B (en) | Volvaria volvacea cultivation method | |
| CN102617208B (en) | Edible fungus residue organic fertilizer raw materials and preparation method thereof | |
| CN107857689A (en) | The technique that a kind of bioanalysis prepares bio-organic fertilizer using chicken manure | |
| CN102249756A (en) | Biological organic fertilizer using orange peel residue as main raw material and preparation method thereof | |
| CN105967947A (en) | Ferment biological bacterial fertilizer using processing by-products of preserved fruits of apples as main raw material | |
| CN105152705A (en) | Bio-organic fertilizer based on agricultural and forestry wastes and production method of bio-organic fertilizer | |
| CN107912267A (en) | A kind of preparation method of Chinese fir seedling medium | |
| CN107646616A (en) | A kind of preparation method of floriculture substrate | |
| CN110256145A (en) | A kind of tobacco organic fertilizer and preparation method thereof | |
| CN108821875A (en) | A kind of preparation method of high-activity microorganism fertilizer | |
| CN102826879A (en) | Production method of active microbial organic fertilizer | |
| CN104099281A (en) | Environment-friendly compound microbial agent | |
| CN104744088A (en) | Method for producing organic fertilizer by using mushroom residues as main raw material | |
| CN101897272A (en) | Integral resource recycling method of bagasse | |
| CN106892701A (en) | A kind of method that utilization pleurotus eryngii bacteria residue cultivates elegant precious mushroom | |
| CN103351188B (en) | Preparation method of agricultural organic fertilizer by treating pure straws as raw material | |
| CN101735818B (en) | Microbial soil conditioner prepared from edible fungus bran and preparation method thereof | |
| CN107304406A (en) | A kind of microorganism formulation preparation method for making crop material rotten | |
| CN111777440A (en) | Method for producing organic fertilizer by fermenting corn straws and cow dung | |
| CN108566873A (en) | A kind of vegetable soilless culture matrix and the preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171031 |