CN107295892A - A kind of method of eryuelan purification and rejuvenation and supporting isolation solarium - Google Patents

A kind of method of eryuelan purification and rejuvenation and supporting isolation solarium Download PDF

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Publication number
CN107295892A
CN107295892A CN201710680587.9A CN201710680587A CN107295892A CN 107295892 A CN107295892 A CN 107295892A CN 201710680587 A CN201710680587 A CN 201710680587A CN 107295892 A CN107295892 A CN 107295892A
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China
Prior art keywords
eryuelan
honeybee
isolation
tube
solarium
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CN201710680587.9A
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Chinese (zh)
Inventor
张晓冬
隋学艳
王效睦
李润芳
王栋
李湛
杨永义
田茜
丁汉凤
刘全兰
陈夫山
黄龙江
刘世华
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Shandong Crop Germplasm Resource Center
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Shandong Crop Germplasm Resource Center
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Priority to CN201710680587.9A priority Critical patent/CN107295892A/en
Publication of CN107295892A publication Critical patent/CN107295892A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility

Abstract

The invention discloses a kind of method of eryuelan purification and rejuvenation.It include isolation solarium build, etc. line-spacing sow, disposably topdress, remove hybrid strain, wall honeybee dispensing, seed harvest etc. step.Present invention also offers a kind of supporting isolation solarium, including:Awning film or sunshade net, Isolation net cover and the support frame for supporting Isolation net cover.The present invention is low temperature resistant using wall honeybee, and pollination speed is fast, and Pollination Effect is good, and the advantages of management is easy coordinates the use of isolation solarium, can effectively improve the efficiency of eryuelan purification and rejuvenation, and the requirement of seed production can be met again.

Description

A kind of method of eryuelan purification and rejuvenation and supporting isolation solarium
Technical field
The present invention relates to green manure or ornamental crops breeding technical field, particularly one kind is carried using wall honeybee to eryuelan The method of pure rejuvenation, the invention further relates to supporting isolate solarium with its.
Background technology
Eryuelan (Orychophragmus violaceus), also known as two pale blue, Orychophragmus violaceus, Cruciferae Orychophragmus violaceus are drafted a document This plant, is distributed widely in northern China most area, and cold-resistant, drought-enduring, shade tolerant, pest and disease damage is few, be the northern area of China not The winter that can be obtained sees green manure or the ornamental plant for seeing flower in green, early spring more.Eryuelan seed oil content is up to 45% or so, its fat In acid composition, erucic acid, linolenic acid content are low, and palmitic acid, oleic acid and linoleic content are more than the 85% of total fat, therefore, Eryuelan is high linoleic acid low erucic acid germplasm, and its seed oil is edible oil best in quality, is especially suitable for cardiovascular patient food With.So, eryuelan is a kind of economic value very high multipurpose green manure or ornamental crops.
The normal pattern of eryuelan is aubergine, and appreciation effect is splendid.But, February Cymbidium entomophilous flower crop, Natural cross pollinatio It is solid normal, and natural cracking after the kind pod maturation of eryuelan, seed is with falling into soil, therefore, and eryuelan is very easy to out Existing variet complexity, and then cause variety deterioration, pattern deteriorates to lavender, or even white, has a strong impact on appreciation effect and utilization Value.For this reason, it may be necessary to periodically carry out purification and rejuvenation work.Colony selects and Single-plant selection is the conventional side of crop varieties purification and rejuvenation Method, colony's selection refers to removal hybrid strain, weak plant from the varietal population degenerated, passes through individual plant impurity elimination, com-parison and analysis, mixing Expand the process that numerous, seed production etc. obtains high-purity original seed.Colony's selection can be completed in a relatively short time purification and rejuvenation and former Production is planted, the purification and rejuvenation of eryuelan are especially suitable for.
Eryuelan self-fruitful rate is very low, needs artificial supplementary pollination or carries out supple-mentary pollination by Pollinating Insect.Manually Supple-mentary pollination is time-consuming, easily goes here and there powder, and eryuelan stalk is more crisp, and artificial supplementary pollination easily damages plant, production of hybrid seeds effect compared with Difference.Honeybee is Pollinating Insect common at present, and the bee species used in agricultural production have Xiong Feng, middle honeybee, wall honeybee, Qie Ye Honeybee etc..Wherein, the wall honeybee category that wall honeybee belongs in Apoidea (apoidea) Megachilidae (megachilidae) (osmia) it is, the brassicaceous vegetables such as Chinese cabbage, rape, radish, rose family fruit tree and the Mi such as apple, pears, peach, cherry, apricot, Lee The excellent Pollinating Insect of monkey peach etc., it is advantageous that:1 year 1 generation of wall honeybee, Natural Survival, reproductive capacity is strong, it is warm-natured and, without feeding, It has the life in tube nest in 320 days or so in 1 year, lived 40 days or so in pollination activity outside tube nest, be easy to put management in a suitable place to breed, Also the contradiction gone and buy Chinese medicine with fruits and vegetables is avoided naturally, and wall honeybee is adapted to early spring activity, and low temperature resistant, breeding potential is high, and scope of activities is small, pollination Speed is fast, and Pollination Effect is good, and management is easy, can also go out the features such as nest is pollinated even in bad weathers such as rainy days, be very suitable for Spring pollinates.
Patent of invention 201310019004.X discloses a kind of method for pollinating and producing seeds of rapes by osmia, and described wall honeybee is day This Osmia cornifrons, described method includes:1-2 days before rape is bloomed, side is provided with and puts the beehive of honeybee mouthful and moves on to rape system In farming or production of hybrid seeds canopy, and adjacent beehive spacing is 20-30m, and beehive lower surface is far from ground 1.2m;At the end of the rape florescence, wall Honeybee pollination task is completed, and numerous honeybee is terminated, and withdraws beehive.
Patent of invention 201610091452.4 discloses a kind of open country portable Chinese cabbage kind strain selfing Seed Collecting Methods, including Managed before site preparation and fertilization, kind strain field planting, set Isolation net cover, cover Isolation net cover, put wall honeybee, check that benefit puts selfing after wall honeybee, pollination The step such as system's management and kind strain seed harvest, the technical costs is low, easy to operate and can guarantee that the individual plant of the breeding material of acquisition certainly String powder does not occur between friendship seed and other individual plants to mix.
Patent of invention 201510215271.3 discloses a kind of being measured for the high Chinese cabbage crossing combination of efficiently and accurately rate Method, it comprises the following steps:Manage, put after Chinese cabbage strain breeding and sowing, canopy room preparation, cross combination field planting, field planting Prepare before honeybee, be put into wall honeybee, put management after honeybee, seed harvest.
The published Patents of comprehensive analysis, wall honeybee is widely used for the crop in cruciferae such as rape, Chinese cabbage and educated Kind.Though eryuelan belongs to Cruciferae together, breeding objective, habit, biological characteristics, supple-mentary pollination method etc. and other Crop in cruciferae has significant difference.The particularly eryuelan florescence is early, and initial bloom stage temperature is relatively low, after wall honeybee is delivered incubation rate compared with Low, mobility and pollination time are substantially reduced.Have not yet to see the open report for carrying out supple-mentary pollination to eryuelan using wall honeybee Road, also has no the open report purificated and rejuvenated to eryuelan.
The content of the invention
In order to make up the blank of existing eryuelan breeding technique, the invention provides a kind of method of eryuelan purification and rejuvenation And supporting isolation solarium.The present invention is low temperature resistant using wall honeybee, and pollination speed is fast, and Pollination Effect is good, and the advantages of management is easy coordinates The use of detachable isolation solarium, can effectively improve the efficiency of eryuelan purification and rejuvenation, and wanting for seed production can be met again Ask.It is this method simple possible, efficient saving of labor, easily operated.
To solve the above problems, the technical solution adopted by the present invention is as follows:A kind of method of eryuelan purification and rejuvenation, it is special Levying is, comprises the following steps:
(1) isolation solarium is built
Behind rotary cultivator rotary tillage soil 1-2 times, isolation solarium, solarium's surrounding nylon wire are built according to experimental field block size It is pressed into spacing between 15cm-20cm in soil, two isolation solariums and is not less than 1m;
It is preferred that isolation solarium is built in the plot of never plantation eryuelan, preferably using top has the nylon of rain and snow function Solarium, preferably uses the nylon solarium of 40-60 mesh.
(2) the line-spacing sowing such as
The line-spacing drillings, line-spacing 40cm-60cm, the amount of broadcasting 4.5kg/hm such as sowing use2-6kg/hm2, seed level 1cm.
Further, one of for example following A-B of scheme of line-spacing drilling is preferably waited:
A. line-spacing 40cm, the amount of broadcasting 6kg/hm2, seed level 1cm.
The program is applied to subramose orchid species in February.
B. line-spacing 60cm, the amount of broadcasting 4.5kg/hm2, seed level 1cm.
The program is applied to the orchid species in February more than branch.
(3) disposably topdress
Squaring period is according to N 45kg/hm2-60kg/hm2, P2O5 18kg/hm2-25kg/hm2, K2O 40kg/hm2-55kg/ hm2Effective dose topdressed, fertilization depth is 3cm-5cm;According to 800m3/hm2-1200m3/hm2Irrigation volume waters once, Spray insecticide 1-2 times.
It is preferred that Instant chemical fertilizer of simple substance, composite fertilizer or special fertilizer etc., preferably low toxicity broad spectrum pesticide.
(4) hybrid strain is removed
Initial bloom stage removes hybrid strain, diseased plant according to pattern in eryuelan colony, wipes out and has produced seeds pod and bloomed piece.
It is preferred that retaining the eryuelan individual plant that pattern is aubergine.
(5) wall honeybee is delivered
When every daily minimal tcmperature stabilization is more than 15 DEG C, wall honeybee honeybee cocoon is taken out from 0-4 DEG C of refrigerating chamber, is laid in Above honeybee pipe;Honeybee pipe is positioned over weather-proof area without shade, 10cm-15cm above ground level.Honeybee cocoon supply volume be 6-10 only/ 10m2, honeybee pipe supply volume is 3-5/10m2.Eryuelan can be pollinated after wall honeybee hatches.
It is preferred that delivering the Osmia. excavataAlfken or Osmia cornifrons by domestication, preferably 16:Wall honeybee is delivered after 00, is preferably sprayed Apply insecticide and deliver wall honeybee without rainy weather after 7-10 days and in following 2 days.Preferred length 8cm-10cm, diameter 5mm-9mm, two The reed pipe of end opening is as wall honeybee honeybee pipe, and the place North and South direction preferably adjacent to water source places honeybee pipe.
Eryuelan duration of flowering, aphid often occurs, and influence eryuelan is bloomed and the pollination of wall honeybee.If there is aphid, then (generally dusk or morning), which sprays 10% flonicamid water dispersible granules, during wall honeybee returns nest can kill aphid, and wall honeybee is not It is impacted.
(6) seed is harvested
Florescence terminates to remove isolation solarium, reclaims honeybee pipe, and the maturity period to be born pods harvests eryuelan plant, after drying 3~5 days Threshing.Seed moisture content is down to less than 13%, and storage is preserved.
It is optional step in addition, the field management of eryuelan is depending on this season actually occurs situation, and with this area Common technology means can be solved.
Present invention also offers it is a kind of with the above method is matching used isolates solarium, it can realize the effective of eryuelan Isolation and purification and rejuvenation.
One kind isolation solarium, including awning film or sunshade net, Isolation net cover and the support frame for supporting Isolation net cover; Support frame as described above includes VERTICAL TUBE, horizontal tube and push pipe, and the centre of the push pipe is arc-shaped, and the push pipe is length scale The telescopic tube that can be adjusted, the VERTICAL TUBE is the telescopic tube that length scale can be adjusted, and the horizontal tube is that length is big The small telescopic tube that can be adjusted;The top of the VERTICAL TUBE is provided with the connecting tube for inserting the connection push pipe, described In the connecting tube that one end of push pipe is set in the VERTICAL TUBE and the other end of the push pipe is set in corresponding other one The push pipe is fixed in two VERTICAL TUBEs so as to realize in connecting tube in the VERTICAL TUBE;In same a line VERTICAL TUBE is linked together by the horizontal tube and the horizontal tube is intertwined and connected with the VERTICAL TUBE by iron wire;It is mutually flat Row and positioned at being linked together with the push pipe in a line by the horizontal tube and the horizontal tube and the push pipe pass through iron wire It is intertwined and connected;The Isolation net cover is covered on support frame as described above, and the Isolation net cover grow tall side by bayonet unit with The VERTICAL TUBE is snapped connection, and the bayonet unit includes neck, silk thread and buckle, and the neck is fixed by the silk thread On the Isolation net cover, the buckle is fixed in VERTICAL TUBE, and the neck is buckled in the buckle;The awning film leads to VELCRO is crossed to be removable installed on the outer top surface of the Isolation net cover with rain and snow;The sunshade net is removable by VELCRO It is arranged at unloading on the outer top surface of the Isolation net cover to adjust illumination.
It is preferred that, the high side of foreign minister of the Isolation net cover on the high side of outer width by VELCRO with being detachably provided with Awning film is for insulation under microthermal climate.
It is preferred that, the closing of one end of the horizontal tube, and the other end the going out by pipeline and air-heater of the horizontal tube Air port is connected, and is provided with a string of exhaust vents on the horizontal tube along its length.
It is preferred that, the be made up of VERTICAL TUBE and horizontal tube is additionally provided with one end on the length direction of support frame as described above It is provided with the first movable door frame on end face support frame, the first end face support frame, the described first movable door frame and described the The main body of end face support frame is articulated and connected, and the described first movable door frame upper berth is provided with separation net to constitute for convenient turnover First dodge gate.
It is preferred that, the be made up of VERTICAL TUBE and horizontal tube is additionally provided with one end on the length direction of support frame as described above Biend support frame, the second end face support frame is located at the first end face support frame on the length direction of support frame as described above Same one end and be sequentially arranged along the length direction of support frame as described above, be provided with the second end face support frame separation net with will Space in the separation net room is divided into small one and large one two spaces;The second dodge gate is provided with the second end face support frame Frame, the main body of the described second movable door frame and the second end face support frame is articulated and connected, and the described second movable door frame upper berth is set There is separation net to constitute for convenient the second dodge gate passed in and out;First dodge gate constitutes two with second dodge gate and entered Door type structure.
It is preferred that, the Isolation net cover is the nylon guard of 40~60 mesh.
It is preferred that, the VERTICAL TUBE, connecting tube, push pipe and horizontal tube are galvanized pipe.
The beneficial effects of the invention are as follows:
(1) florescence coincide, and input cost is low
Wall honeybee is low temperature resistant, is suitable for spring pollination, is coincide with the eryuelan florescence, and scope of activities is small, and pollination speed is fast, awards Powder effect is good, and management is easy.Present invention design, can be flexible with base area block size using single honeybee cocoon and honeycomb pipe as unit is delivered Adjust supply volume, it is to avoid the excessive dispensing of FCL honeybee in conventional method, greatly save input cost.
(2) purification and rejuvenation efficiency high
Wall honeybee is hatched by honeybee cocoon, will not carry other pollen, and is lived on one's own life, and is not interfere with each other, it is to avoid know clearly mutually Go here and there the possibility of powder, pollination efficiency is high, effectively solve that string powder, recruitment be more, the low problem of setting percentage.By using the present invention Method and supporting isolation solarium, purity increase by 43.23%, output increased 16.62%.Therefore, the present invention can be effectively improved The efficiency of eryuelan purification and rejuvenation, can meet the requirement of seed production again.
(3) it is simple to operate, it is easy to promote
Present invention also offers a kind of supporting isolation solarium, processing raw material are common on the market galvanized pipe and greenhouse Accessory, you can base area block size, adjusts solarium's size, can increase awning film according to weather condition, with rain and snow, and carry again Temperature at the top of high solarium, or increase sunshade net, reconcile illumination, strengthen the incubation rate and pollination ability of wall honeybee.Isolation solarium adds Work is simple with installation method, and production cost is low, is easy to promote.
Brief description of the drawings
Fig. 1 be a kind of isolation solarium provided in an embodiment of the present invention in support frame structural representation;
Fig. 2 is Fig. 1 right view.
In figure:1 push pipe, 2 VERTICAL TUBEs, 3 horizontal tubes, 4 ground.
Embodiment
In order to illustrate the technical characterstic of the present invention, below by embodiment, and Figure of description is combined, to scheme It is illustrated.
Reference picture 1~2, Fig. 1 be a kind of isolation solarium provided in an embodiment of the present invention in support frame structural representation; Fig. 2 is Fig. 1 right view.
Isolate solarium this application provides one kind, including awning film or sunshade net, Isolation net cover and for supporting isolation The support frame of guard;Support frame as described above includes VERTICAL TUBE 2, horizontal tube 3 and push pipe 1, and the centre of the push pipe 1 is arc-shaped, The push pipe 1 is the telescopic tube that can adjust of length scale, the VERTICAL TUBE 2 be length scale can adjust it is scalable Pipe, the horizontal tube 3 is the telescopic tube that length scale can be adjusted;The top of the VERTICAL TUBE 2 is provided with for the company of insertion Connect the connecting tube of the push pipe 1, one end of the push pipe 1 is set in the connecting tube in the VERTICAL TUBE 2 and the push pipe 1 The push pipe 1 is fixed on two so as to realize in the connecting tube that the other end is set in a corresponding other VERTICAL TUBE 2 Described in root in VERTICAL TUBE 2;VERTICAL TUBE 2 in same a line linked together by the horizontal tube 3 and the horizontal tube 3 with The VERTICAL TUBE 2 is intertwined and connected by iron wire;It is parallel to each other and is located at and is connected with the push pipe 1 in a line by the horizontal tube 3 Together and the horizontal tube 3 is intertwined and connected with the push pipe 1 by iron wire;The Isolation net cover is covered on support frame as described above, And the side of growing tall of the Isolation net cover is snapped connection by bayonet unit with the VERTICAL TUBE 2, the bayonet unit includes card Groove, silk thread and buckle, the neck are fixed on the Isolation net cover by the silk thread, and the buckle is fixed on VERTICAL TUBE On 2, the neck is buckled in the buckle;The awning film is removable installed in the Isolation net cover by VELCRO With rain and snow on outer top surface;The sunshade net is removable installed on the outer top surface of the Isolation net cover to adjust by VELCRO Save illumination.
In the application, the lower end of VERTICAL TUBE is inserted in ground 4 to be fixed.
In one embodiment of the application, the high side of foreign minister of the Isolation net cover on the high side of outer width with passing through magic Patch is detachably provided with awning film to be incubated under microthermal climate;When release honeybee is pollinated, if running into low temperature Weather, can reduce the incubation rate of honeybee and the mobility of honeybee, influence pollination efficiency and pollination quality, therefore, when pollination When running into low temperature, whole isolation solarium is wrapped up with awning film, to improve the temperature in isolation solarium.
In one embodiment of the application, one end closing of the horizontal tube 3, and the other end of the horizontal tube 3 is logical Piping is connected with the air outlet of air-heater, and is provided with a string of exhaust vents on the horizontal tube 3 along its length;Work as isolation After solarium is wrapped up by awning film, hot blast is blowed into separation net room using air-heater, is further improved in isolation solarium Temperature.
In one embodiment of the application, it is additionally provided with one end on the length direction of support frame as described above by VERTICAL TUBE The 2 first end face support frames constituted with horizontal tube 3, are provided with the first movable door frame on the first end face support frame, and described the The main body of one movable door frame and the first end face support frame is articulated and connected, the described first movable door frame upper berth be provided with separation net with Constitute for convenient the first dodge gate passed in and out, to be convenient for people to turnover isolation solarium, weeding, fertilising are carried out into isolation solarium And agricultural operation of watering etc., and the not isolation of damage containment solarium.
In one embodiment of the application, it is additionally provided with one end on the length direction of support frame as described above by VERTICAL TUBE The 2 second end face support frames constituted with horizontal tube 3, the second end face support frame is located at described with the first end face support frame Same one end on the length direction of support frame and it is sequentially arranged along the length direction of support frame as described above, the second end face support frame On be provided with separation net the space in the separation net room is divided into small one and large one two spaces;The second end face support frame On be provided with the second movable door frame, the main body of the described second movable door frame and the second end face support frame is articulated and connected, described Second movable door frame upper berth is provided with separation net to constitute for convenient the second dodge gate passed in and out;First dodge gate with it is described Second dodge gate constitutes two and enters door type structure;Set two to enter door type structure, be in order to which by inside and outside twice separation net door, reduction exists Honeybee improves the practicality of the isolation solarium from the probability and quantity of the escape in door during switch gate.
In one embodiment of the application, the Isolation net cover is the nylon guard of 40~60 mesh.
In one embodiment of the application, the VERTICAL TUBE 2, connecting tube, push pipe 1 and horizontal tube 3 are galvanized pipe.
Following embodiments uses above-mentioned isolation solarium.
A kind of method purificated and rejuvenated using wall honeybee to eryuelan of the present invention, is comprised the following steps:
(1) isolation solarium is built
The plot for never planting eryuelan is chosen, behind rotary cultivator rotary tillage soil 1-2 times, is taken according to experimental field block size Build as shown in Figure 1-2, top has the nylon solarium of the 40-60 mesh of rain and snow function, solarium's surrounding nylon wire press-in soil Middle 15cm-20cm, isolation solarium's spacing is not less than 1m.
(2) the line-spacing sowing such as
A. the line-spacing drilling, line-spacing 40cm, the amount of broadcasting 6kg/hm such as sowing use2, seed level 1cm.
The program is applied to subramose orchid species in February.
B. the line-spacing drilling, line-spacing 60cm, the amount of broadcasting 4.5kg/hm such as sowing use2, seed level 1cm.
The program is applied to the orchid species in February more than branch.
(3) disposably topdress
Squaring period is according to N 45kg/hm2-60kg/hm2, P2O5 18kg/hm2-25kg/hm2, K2O 40kg/hm2-55kg/ hm2Effective dose impose Instant chemical fertilizer of simple substance, composite fertilizer or special fertilizer etc., fertilization depth is 3cm-5cm;According to 800m3/ hm2-1200m3/hm2Irrigation volume waters once, sprays low toxicity broad spectrum pesticide 1-2 times.
(4) hybrid strain is removed
Initial bloom stage removes hybrid strain, diseased plant according to pattern in eryuelan colony, retains the eryuelan list that pattern is aubergine Strain, wipes out and has produced seeds pod and bloomed piece.
(5) wall honeybee is delivered
After spraying insecticide 7-10 days, when every daily minimal tcmperature is stably more than 15 DEG C, and without rainy weather in 2 days futures When, it will be taken out by the Osmia. excavataAlfken or Osmia cornifrons honeybee cocoon of domestication from 0-4 DEG C of refrigerating chamber, 16:Tiled after 00 Above honeybee pipe.Honeybee pipe is length 8cm-10cm, diameter 5mm-9mm, the reed pipe of both ends open, and North and South direction is positioned over close Water source and weather-proof area without shade, 10cm-15cm above ground level.Honeybee cocoon supply volume is 6-10/10m2, honeybee pipe supply volume For 3-5/10m2
Eryuelan duration of flowering, aphid often occurs, and influence eryuelan is bloomed and the pollination of wall honeybee.If there is aphid, then (generally dusk or morning), which sprays 10% flonicamid water dispersible granules, during wall honeybee returns nest can kill aphid, and wall honeybee is not It is impacted.
(6) seed is harvested
Florescence terminates to remove solarium, reclaims honeybee pipe, and the maturity period to be born pods harvests eryuelan plant, is taken off after drying 3~5 days Grain.Seed moisture content is down to less than 13%, and storage is preserved.
It is optional step in addition, the field management of eryuelan is depending on this season actually occurs situation, and with this area Common technology means can be solved.
In order to verify specific application effect, the present invention has carried out following experiment respectively:
In specific process of the test, the length and width size of isolation solarium is 16m × 4m, wherein:Frame system (VERTICAL TUBE, Connecting tube, push pipe and horizontal tube) purchased by market galvanized pipe self-control form, awning film is limited by the emerging greenhouse facilities of Jinan spring Company provides, and is market common-use size, and nylon wire is that 40 mesh are produced by Taizhou plain Ke Nong screen clothes factory.Rotary cultivator (1GQN-125 types) Produced by Weifang Feng Min agricultural equipments Co., Ltd, and power is provided by Dongfanghong 504 (group is dragged by China one).Fertilizer used is Urea (46-0-0) is produced by Lunan Chemical Fertilizer Factory, Shandong, and MAP (11-44-0) is produced by Hubei Xiang Yun chemical industry joint-stock company, Potassium sulfate (0-0-51) is produced by Guotou Xinjiang Luobubu Potassium Salt LLC, and insecticide used is tetramethrin (0.6%) Produced by HeBei KangDa Co., Ltd.
February, orchid species were blue No. 1 (the pale reddish brown kind of Shandong Province's Germplasm Resources of Farm Crop seed selection, branch is less) in Shandong, in Ji Nanshi Licheng District, which has been planted 3 years, degenerates seriously, and in June, 2014 gathers seed P, in October, 2014 in June, 2015 in Ji Nanshi Licheng District is carried out to P purificates and rejuvenates, and harvests seed F1, plants F1 in October, 2015, observe pattern in March, 2016, counts Hybrid strain rate.
(1) embodiment
On October 9th, 2014 sows Shandong No. 1 seed P of orchid, sows the line-spacing drillings, line-spacing 40cm, the amount of broadcasting 6kg/ such as use hm2, seed level 1cm.On March 8th, 2015 imposes urea 88kg/hm2, MAP 41kg/hm2, potassium sulfate 79kg/hm2, fertilising Depth is 4cm, according to 1000m3/hm2Irrigation volume waters once, sprays tetramethrin (0.6%) once.On March 15th, 2015, system Meter is calculated after hybrid strain rate and purity, and impurity elimination and wiping out has produced seeds pod and bloomed piece, 8/10m of dispensing wall honeybee honeybee cocoon2, deliver honeybee 4/10m of nest pipe2.On June 1st, 2015, harvest, threshing after drying 4 days is weighed, and calculates grain yield (kg/m2).2015 10 The moon 12, Shandong No. 1 seed F1 of orchid is sowed, on March 13rd, 2016, counts and calculates hybrid strain rate and purity.
Hybrid strain rate statistical calculation method:Delimit 3 4m2The statistics cell of (4m × 1m), statistics total strain number and hybrid strain number, point Average value is not calculated.Hybrid strain rate (%)=hybrid strain number/total strain number × 100%.Purity (%)=100- hybrid strain rates.
(2) comparative example
Comparative example is not in addition to separation net room is built, the equal be the same as Example 1 of other conditions including impurity elimination ratio, with implementation Example 1 is carried out simultaneously with ground, and 5m is spaced between two plot.
The field experiment result of embodiment and comparative example is as shown in table 1:
Hybrid strain rate, purity and the seed production of the embodiment of table 1. and comparative example are contrasted
Hybrid strain rate, purity and the seed production contrast of embodiment and comparative example are shown in Table 1, and as shown in Table 1, the present invention can be by two The moon, blue hybrid strain rate reduced to 13.29% from initial 74.12%, and 66.32% is reduced compared with comparative example;Purity is from initial 25.88% brings up to 86.71%, and 43.23% is improved compared with comparative example;Eryuelan variety rejuvenation effect shows very much Write, at the same time, seed production also improves 16.62%.
Simply the preferred embodiment of the present invention described above, for those skilled in the art, Without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications are also regarded as this hair Bright protection domain.

Claims (10)

1. a kind of method of eryuelan purification and rejuvenation, it is characterized in that, comprise the following steps:
(1) isolation solarium is built
Behind rotary cultivator rotary tillage soil 1-2 times, 15cm-20cm in isolation solarium, solarium's surrounding press-in soil is built;
(2) the line-spacing sowing such as
The line-spacing drillings, line-spacing 40cm-60cm, the amount of broadcasting 4.5kg/hm such as sowing use2-6kg/hm2, seed level 1cm;
(3) disposably topdress
Eryuelan squaring period is according to N 45kg/hm2-60kg/hm2, P2O5 18kg/hm2-25kg/hm2, K2O 40kg/hm2- 55kg/hm2Effective dose topdressed, fertilization depth is 3cm-5cm;According to 800m3/hm2-1200m3/hm2Irrigation volume waters Once, insecticide is sprayed 1-2 times;
(4) hybrid strain is removed
Initial bloom stage removes hybrid strain, diseased plant according to pattern in eryuelan colony, wipes out and has produced seeds pod and bloomed piece;
(5) wall honeybee is delivered
When every daily minimal tcmperature stabilization is more than 15 DEG C, wall honeybee honeybee cocoon is taken out from refrigerating chamber, is laid in above honeybee pipe;Honeybee Pipe is positioned over weather-proof area without shade, 10cm-15cm above ground level;Honeybee cocoon supply volume is 6-10/10m2, honeybee pipe dispensing number Measure as 3-5/10m2;Eryuelan is pollinated after wall honeybee hatches;
(6) seed is harvested
Florescence removes isolation solarium after terminating, and reclaims honeybee pipe, and the maturity period to be born pods harvests eryuelan plant, threshing after drying;Treat Seed moisture content is down to less than 13%, and storage is preserved.
2. a kind of method of eryuelan purification and rejuvenation as claimed in claim 1, it is characterized in that, the step 1) use top tool There is the nylon solarium of rain and snow function;Spacing is not less than 1m between two isolation solariums.
3. a kind of method of eryuelan purification and rejuvenation as claimed in claim 1, it is characterized in that, the step (2) is for branch Few orchid species in February, line-spacing 40cm, the amount of broadcasting 6kg/hm2, seed level 1cm.
4. a kind of method of eryuelan purification and rejuvenation as claimed in claim 1, it is characterized in that, the step (3) is for branch Many orchid species in February, line-spacing 60cm, the amount of broadcasting 4.5kg/hm2, seed level 1cm.
5. a kind of method of eryuelan purification and rejuvenation as claimed in claim 1, it is characterized in that, the step (4) retains pattern For the eryuelan individual plant of aubergine.
6. a kind of method of eryuelan purification and rejuvenation as claimed in claim 1, it is characterized in that, the step (5) is using process The Osmia. excavataAlfken or Osmia cornifrons of domestication, 16:Delivered after 00.
7. a kind of method of eryuelan purification and rejuvenation as claimed in claim 1, it is characterized in that, the step (5) uses length 8cm-10cm, diameter 5mm-9mm, the reed pipe of both ends open place honeybee as honeybee pipe in the place North and South direction close to water source Pipe.
8. a kind of method in 1-7 with claim described in any one is matching used to isolate solarium, it is characterized in that, including it is big Canopy film or sunshade net, Isolation net cover and the support frame for supporting Isolation net cover;Support frame as described above includes VERTICAL TUBE, horizontal tube And push pipe, the centre of the push pipe is arc-shaped, and the push pipe is the telescopic tube that length scale can be adjusted, described vertical The telescopic tube that can be adjusted for length scale is managed, the horizontal tube is the telescopic tube that length scale can be adjusted;It is described perpendicular The top of straight tube is provided with the connecting tube for inserting the connection push pipe, and one end of the push pipe is set in the VERTICAL TUBE Connecting tube on and the other end of the push pipe be set in the connecting tube in a corresponding other VERTICAL TUBE so as to real Now the push pipe is fixed in two VERTICAL TUBEs;VERTICAL TUBE in same a line is connected to one by the horizontal tube Rise and the horizontal tube is intertwined and connected with the VERTICAL TUBE by iron wire;It is parallel to each other and is located at and passes through institute with the push pipe in a line State that horizontal tube is linked together and the horizontal tube is intertwined and connected with the push pipe by iron wire;The Isolation net cover covers on described On support frame, and the side of growing tall of the Isolation net cover is snapped connection by bayonet unit with the VERTICAL TUBE, the buckle dress Put including neck, silk thread and buckle, the neck is fixed on the Isolation net cover by the silk thread, and the buckle is fixed In VERTICAL TUBE, the neck is buckled in the buckle;The awning film by VELCRO be removable installed in it is described every With rain and snow on the outer top surface of off-network cover;The sunshade net is removable installed in the outer top of the Isolation net cover by VELCRO To adjust illumination on face.
9. the matching used isolation solarium of method of eryuelan as claimed in claim 8 purification and rejuvenation, it is characterized in that, it is described every The high side of the foreign minister of off-network cover by VELCRO on the high side of outer width with being detachably provided with awning film for microthermal climate Lower insulation.
10. the matching used isolation solarium of method of eryuelan purification and rejuvenation as claimed in claim 8, it is characterized in that, it is described It is additionally provided with the first end face support frame being made up of VERTICAL TUBE and horizontal tube on one end on the length direction of support frame, described the The first movable door frame is provided with end face support frame, the main body of the described first movable door frame and the first end face support frame is cut with scissors Connect in succession, the described first movable door frame upper berth is provided with separation net to constitute for convenient the first dodge gate passed in and out.
CN201710680587.9A 2017-08-10 2017-08-10 A kind of method of eryuelan purification and rejuvenation and supporting isolation solarium Withdrawn CN107295892A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108834877A (en) * 2018-06-27 2018-11-20 黄冈市农业科学院 A kind of method of the radish wall bee pollination purification and rejuvenation production of hybrid seeds
CN110915580A (en) * 2019-10-22 2020-03-27 湖南金源种业有限公司 Pollen drift prevention method for rice breeding
CN113692857A (en) * 2021-08-26 2021-11-26 中国农业科学院农业资源与农业区划研究所 Efficient orychophragmus violaceus harvesting method and device

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108834877A (en) * 2018-06-27 2018-11-20 黄冈市农业科学院 A kind of method of the radish wall bee pollination purification and rejuvenation production of hybrid seeds
CN110915580A (en) * 2019-10-22 2020-03-27 湖南金源种业有限公司 Pollen drift prevention method for rice breeding
CN113692857A (en) * 2021-08-26 2021-11-26 中国农业科学院农业资源与农业区划研究所 Efficient orychophragmus violaceus harvesting method and device

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Application publication date: 20171027