CN107285890A - A kind of preparation method of pleurotus nebrodensis culture medium - Google Patents

A kind of preparation method of pleurotus nebrodensis culture medium Download PDF

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Publication number
CN107285890A
CN107285890A CN201710623861.9A CN201710623861A CN107285890A CN 107285890 A CN107285890 A CN 107285890A CN 201710623861 A CN201710623861 A CN 201710623861A CN 107285890 A CN107285890 A CN 107285890A
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China
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parts
pleurotus nebrodensis
culture medium
preparation
soil extract
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CN201710623861.9A
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Chinese (zh)
Inventor
高翔
葛修梅
张振林
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Anhui Noah Agriculture Co Ltd
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Anhui Noah Agriculture Co Ltd
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Priority to CN201710623861.9A priority Critical patent/CN107285890A/en
Publication of CN107285890A publication Critical patent/CN107285890A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention discloses a kind of preparation method of pleurotus nebrodensis culture medium, comprise the following steps:(1)Weighing,(2)Solarization material,(3)Soil extract preparation,(4)Batch mixing,(5)Pack sterilizing.The present invention has carried out rational selection to the material composition of pleurotus nebrodensis culture medium and arranged in pairs or groups, final obtained artificial cultivation matrix can effectively lift the growth quality and yield of Pleurotus nebrodensis, and the growth cycle of Pleurotus nebrodensis mycelia is shortened, and the Pleurotus nebrodensis quality brought out is excellent, in good taste, high financial profit.

Description

A kind of preparation method of pleurotus nebrodensis culture medium
Technical field
The invention belongs to eat the cultivation field of mushroom, and in particular to a kind of preparation method of pleurotus nebrodensis culture medium.
Background technology
Pleurotus nebrodensis is Pleurotus eryngii var. nebrodensis, also known as Pleurotus nebrodensis, white Pleurotus ferulae Lanzi, pleurotus eryngii leucismus color kind.Pleurotus nebrodensis, Bai Ling Mushroom, wing abalone mushroom are trade name.In Xinjiang, because being born in the large-scale Chinese medicine of Umbelliferae --- on the plant of asafoetide, habit among the people claims day Mountain god mushroom.Pleurotus nebrodensis has several different shapes.In the market Pleurotus nebrodensis salable refers to stem tubbiness, and cap is especially big, is similar to palm The kind of shape.Stem is longer, cap kind less than normal, then general not as Pleurotus nebrodensis operation.
With the excavation of people, the quantity of wild Pleurotus nebrodensis is few, in order to meet need of the in the market for Pleurotus nebrodensis Ask, it is first many by manually being cultivated.Cultivation matrix is necessary material in artificial cultivation, and the raw material of existing cultivation matrix Composition is single, and proportioning is unreasonable, causes the cultivating rate of Pleurotus nebrodensis rod relatively low, its biological transformation ratio is only 80% or so, be have impact on The economic benefit of artificial cultivation, this problem, which is needed badly, to be improved.
The content of the invention
The purpose of the present invention is that there is provided a kind of preparation method of pleurotus nebrodensis culture medium the problem of being directed to existing.
The present invention is achieved by the following technical solutions:
A kind of preparation method of pleurotus nebrodensis culture medium, comprises the following steps:
(1)Weighing:It is standby that material is weighed by following correspondence parts by weight:25 ~ 30 parts of wood chips, 65 ~ 70 parts of corncobs, 4 ~ 7 parts of pine needles Powder, 10 ~ 13 parts of coconut shell flours, 5 ~ 10 parts of wheat brans, 2 ~ 4 parts of broad bean powders, 3 ~ 5 parts of talcum powder, 2 ~ 3 parts of attapulgites;
(2)Shine material:By step(1)The raw material weighed is put in exposure 2 ~ 3 days under the sun, after the completion of collect standby;
(3)It is prepared by soil extract:The plantation field soil of continuous plantation peach 5 years or more is taken, then with 8 ~ 10 times of its quality Clear water jointly mixing be put into agitator tank, high-speed stirred handle 15 ~ 20min after stand 1 ~ 1.5h again, finally filtered, institute It is soil extract to obtain filtrate;
(4)Batch mixing:By step(2)Shining each composition after material, mixing is put into tank jointly, and step is added thereto(3)Obtained Soil extract, is stirred continuously that to mix to overall water content be to stop applying soil extract after 58 ~ 63%, finally takes out Mixture is standby;
(5)Pack sterilizing:By step(4)The mixture of gained is fitted into polyethylene plastic bag, after the completion of sterilized, finally take Go out to be cooled to room temperature.
Further, step(1)Described in wood chip chip size be 0.5 ~ 1cm, described corncob, the grain of coconut shell flour Footpath is 150 ~ 200 mesh.
Further, step(3)Described in high-speed stirred speed be 1300 ~ 1500 revs/min.
Further, step(5)Described in sterilization treatment be utilize ultraviolet irradiation sterilization.
Only it is made up of more than existing pleurotus nebrodensis culture medium compositions such as wood chip, wheat bran, cotton seed hulls, although it can effectively be protected The nutrient growth of Pleurotus nebrodensis is demonstrate,proved, but for quality-improving DeGrains such as yield, the cultivating rates of Pleurotus nebrodensis, the present invention is directed to this Problem is improved, and having carried out special selection to the raw material composition in cultivation matrix arranges in pairs or groups, wherein the wood chip added, corn The nutrition supply of Pleurotus nebrodensis has been effectively ensured in core, coconut shell flour, wheat bran, broad bean powder, and the addition of pine needle meal improves cultivation matrix Environmental security, reduces the incidence of pest and disease damage, with the addition of soil extract again when batch mixing is prepared, and soil used is Peach has continuously planted the soil of 5 years and the above, generally there is continuous cropping obstacle in plantation, i.e., preceding stubble plants a kind of crop for many years, It is follow-up to carry out that growth is influenceed during same origin plantation again, because the crop can secrete some materials even certainly in planting for many years Malicious composition, influences the growth of succession crop, this effect influences substantially between same origin, if but the non-preceding stubble of the same race of succession crop Crop, is possible to that the growth of succession crop can be promoted on the contrary, the foundation of this namely present crop rotation plantation, plants year after year after peach Soil in contain more phenols, terpene, the bioactive substance do not verified etc., although it is for the poisonous secondary shadow of peach itself Ring, but applicant is found surprisingly that such material can be effectively promoted the growth of Pleurotus ferulae mycelium, coordinates being total to for remaining composition Same-action, enhances the quality and economic benefit of Pleurotus nebrodensis artificial cultivation.
The present invention has advantages below compared with prior art:
The present invention has carried out rational selection to the material composition of pleurotus nebrodensis culture medium and arranged in pairs or groups, final obtained artificial cultivation base Mass-energy effectively lifts the growth quality and yield of Pleurotus nebrodensis, and shortens the growth cycle of Pleurotus nebrodensis mycelia again, brings out Pleurotus nebrodensis quality is excellent, in good taste, high financial profit.
Embodiment
Embodiment 1
A kind of preparation method of pleurotus nebrodensis culture medium, comprises the following steps:
(1)Weighing:It is standby that material is weighed by following correspondence parts by weight:25 portions of wood chips, 65 portions of corncobs, 4 portions of pine needle meals, 10 portions of coconut palms Shell powder, 5 parts of wheat brans, 2 parts of broad bean powders, 3 parts of talcum powder, 2 parts of attapulgites;
(2)Shine material:By step(1)The raw material weighed is put in exposure 2 days under the sun, after the completion of collect standby;
(3)It is prepared by soil extract:The plantation field soil of continuous plantation peach 5 years or more is taken, then with 8 times of its quality Mixing is put into agitator tank clear water jointly, is stood 1h again after high-speed stirred processing 15min, is finally filtered, gained filtrate is For soil extract;
(4)Batch mixing:By step(2)Shining each composition after material, mixing is put into tank jointly, and step is added thereto(3)Obtained Soil extract, is stirred continuously that to mix to overall water content be to stop applying soil extract after 58 ~ 59%, finally takes out Mixture is standby;
(5)Pack sterilizing:By step(4)The mixture of gained is fitted into polyethylene plastic bag, after the completion of sterilized, finally take Go out to be cooled to room temperature.
Further, step(1)Described in wood chip chip size be 0.5 ~ 1cm, described corncob, the grain of coconut shell flour Footpath is 150 ~ 200 mesh.
Further, step(3)Described in high-speed stirred speed be 1300 revs/min.
Further, step(5)Described in sterilization treatment be utilize ultraviolet irradiation sterilization.
Embodiment 2
A kind of preparation method of pleurotus nebrodensis culture medium, comprises the following steps:
(1)Weighing:It is standby that material is weighed by following correspondence parts by weight:28 portions of wood chips, 67 portions of corncobs, 6 portions of pine needle meals, 12 portions of coconut palms Shell powder, 8 parts of wheat brans, 3 parts of broad bean powders, 4 parts of talcum powder, 2.5 parts of attapulgites;
(2)Shine material:By step(1)The raw material weighed is put in exposure 2 days under the sun, after the completion of collect standby;
(3)It is prepared by soil extract:The plantation field soil of continuous plantation peach 5 years or more is taken, then with 9 times of its quality Mixing is put into agitator tank clear water jointly, is stood 1.2h again after high-speed stirred processing 18min, is finally filtered, gained filtrate As soil extract;
(4)Batch mixing:By step(2)Shining each composition after material, mixing is put into tank jointly, and step is added thereto(3)Obtained Soil extract, is stirred continuously that to mix to overall water content be to stop applying soil extract after 60 ~ 61%, finally takes out Mixture is standby;
(5)Pack sterilizing:By step(4)The mixture of gained is fitted into polyethylene plastic bag, after the completion of sterilized, finally take Go out to be cooled to room temperature.
Further, step(1)Described in wood chip chip size be 0.5 ~ 1cm, described corncob, the grain of coconut shell flour Footpath is 150 ~ 200 mesh.
Further, step(3)Described in high-speed stirred speed be 1400 revs/min.
Further, step(5)Described in sterilization treatment be utilize ultraviolet irradiation sterilization.
Embodiment 3
A kind of preparation method of pleurotus nebrodensis culture medium, comprises the following steps:
(1)Weighing:It is standby that material is weighed by following correspondence parts by weight:30 portions of wood chips, 70 portions of corncobs, 7 portions of pine needle meals, 13 portions of coconut palms Shell powder, 10 parts of wheat brans, 4 parts of broad bean powders, 5 parts of talcum powder, 3 parts of attapulgites;
(2)Shine material:By step(1)The raw material weighed is put in exposure 3 days under the sun, after the completion of collect standby;
(3)It is prepared by soil extract:The plantation field soil of continuous plantation peach 5 years or more is taken, then with 10 times of its quality Mixing is put into agitator tank clear water jointly, is stood 1.5h again after high-speed stirred processing 20min, is finally filtered, gained filtrate As soil extract;
(4)Batch mixing:By step(2)Shining each composition after material, mixing is put into tank jointly, and step is added thereto(3)Obtained Soil extract, is stirred continuously that to mix to overall water content be to stop applying soil extract after 62 ~ 63%, finally takes out Mixture is standby;
(5)Pack sterilizing:By step(4)The mixture of gained is fitted into polyethylene plastic bag, after the completion of sterilized, finally take Go out to be cooled to room temperature.
Further, step(1)Described in wood chip chip size be 0.5 ~ 1cm, described corncob, the grain of coconut shell flour Footpath is 150 ~ 200 mesh.
Further, step(3)Described in high-speed stirred speed be 1500 revs/min.
Further, step(5)Described in sterilization treatment be utilize ultraviolet irradiation sterilization.
Comparative example 1
This comparative example 1 compared with Example 2, is saving step(3)Soil extract preparation manipulation, in step(4)Batch mixing During processing with etc. mass parts clear water replace soil extract, method and step all same in addition.
In order to contrast effect of the present invention, above-described embodiment 2, the corresponding cultivation matrix of comparative example 1 are respectively charged into width In 25 ~ 30cm, long 45 ~ 50cm polyethylene plastic bag, identical inoculation processing is then carried out again, bacterium germination room is put into after finishing It is interior, 4 layers are stacked, it is 80 ~ 90% to keep the ambient humidity in bacterium germination room, temperature is 24 ~ 27 DEG C, after heat and moisture preserving is handled 32 ~ 36 days Mushroom producing room is transferred to, growth can adopt mushroom in 10 days or so under conditions of 15 ~ 19 DEG C.The artificial cultivation data of two groups of experiments are specific Contrast is as shown in table 1 below:
Table 1
Biological transformation ratio(%) Average monomer weight(g) Cultivating rate(%) The mycelia purseful time(My god)
Embodiment 2 92.7 237 100 21
Comparative example 1 85.4 186 100 28
Growing and cultivation matrix of the present invention serves good facilitation for the growth of Pleurotus nebrodensis it can be seen from upper table 1, right Soil extract is not added with than embodiment 1, the speed of growth of its mycelia is slower, and biological transformation ratio is relatively low, and passes through specially treated The embodiment of the present invention 2 be obviously improved the effect above, improve economic benefit and the market competitiveness well, promotional value compared with It is high.

Claims (4)

1. a kind of preparation method of pleurotus nebrodensis culture medium, it is characterised in that comprise the following steps:
(1)Weighing:It is standby that material is weighed by following correspondence parts by weight:25 ~ 30 parts of wood chips, 65 ~ 70 parts of corncobs, 4 ~ 7 parts of pine needles Powder, 10 ~ 13 parts of coconut shell flours, 5 ~ 10 parts of wheat brans, 2 ~ 4 parts of broad bean powders, 3 ~ 5 parts of talcum powder, 2 ~ 3 parts of attapulgites;
(2)Shine material:By step(1)The raw material weighed is put in exposure 2 ~ 3 days under the sun, after the completion of collect standby;
(3)It is prepared by soil extract:The plantation field soil of continuous plantation peach 5 years or more is taken, then with 8 ~ 10 times of its quality Clear water jointly mixing be put into agitator tank, high-speed stirred handle 15 ~ 20min after stand 1 ~ 1.5h again, finally filtered, institute It is soil extract to obtain filtrate;
(4)Batch mixing:By step(2)Shining each composition after material, mixing is put into tank jointly, and step is added thereto(3)Obtained Soil extract, is stirred continuously that to mix to overall water content be to stop applying soil extract after 58 ~ 63%, finally takes out Mixture is standby;
(5)Pack sterilizing:By step(4)The mixture of gained is fitted into polyethylene plastic bag, after the completion of sterilized, finally take Go out to be cooled to room temperature.
2. a kind of preparation method of pleurotus nebrodensis culture medium according to claim 1, it is characterised in that step(1)Middle institute The wood chip chip size stated is 0.5 ~ 1cm, and described corncob, the particle diameter of coconut shell flour are 150 ~ 200 mesh.
3. a kind of preparation method of pleurotus nebrodensis culture medium according to claim 1, it is characterised in that step(3)Middle institute The speed for the high-speed stirred stated is 1300 ~ 1500 revs/min.
4. a kind of preparation method of pleurotus nebrodensis culture medium according to claim 1, it is characterised in that step(5)Middle institute The sterilization treatment stated is to utilize ultraviolet irradiation sterilization.
CN201710623861.9A 2017-07-27 2017-07-27 A kind of preparation method of pleurotus nebrodensis culture medium Pending CN107285890A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102807418A (en) * 2011-05-31 2012-12-05 王来战 Preparation method for pleurotus nebrodensis cultivation material
CN103964934A (en) * 2013-02-02 2014-08-06 天津市蓟县果河食用菌专业合作社 Pleurotus nebrodensis fungus rod and preparation method thereof
CN105399471A (en) * 2015-11-30 2016-03-16 陶超杰 Pleurotus nebrodensis nutrition bag and preparation method thereof
CN105622265A (en) * 2015-12-28 2016-06-01 常州思宇环保材料科技有限公司 Method for preparing biological soil conditioner by utilizing edible mushroom dregs
CN106591130A (en) * 2015-10-20 2017-04-26 青岛力天宏泰新能源科技有限公司 Preparation method of soil extract

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102807418A (en) * 2011-05-31 2012-12-05 王来战 Preparation method for pleurotus nebrodensis cultivation material
CN103964934A (en) * 2013-02-02 2014-08-06 天津市蓟县果河食用菌专业合作社 Pleurotus nebrodensis fungus rod and preparation method thereof
CN106591130A (en) * 2015-10-20 2017-04-26 青岛力天宏泰新能源科技有限公司 Preparation method of soil extract
CN105399471A (en) * 2015-11-30 2016-03-16 陶超杰 Pleurotus nebrodensis nutrition bag and preparation method thereof
CN105622265A (en) * 2015-12-28 2016-06-01 常州思宇环保材料科技有限公司 Method for preparing biological soil conditioner by utilizing edible mushroom dregs

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