CN107271433B - A kind of luminous measurement method of single reaction cup - Google Patents
A kind of luminous measurement method of single reaction cup Download PDFInfo
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- CN107271433B CN107271433B CN201710507536.6A CN201710507536A CN107271433B CN 107271433 B CN107271433 B CN 107271433B CN 201710507536 A CN201710507536 A CN 201710507536A CN 107271433 B CN107271433 B CN 107271433B
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- reaction cup
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- top cover
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- Life Sciences & Earth Sciences (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention relates to a kind of luminous measurement method of single reaction cup, following steps: S1: starting switch lid actuator makes top cover open measuring chamber opening, and reaction cup is sent into the accommodating chamber of measuring chamber；S2: starting switch lid actuator makes top closure measuring chamber be open, and starting rotary drive is overlapped the through-hole on baffle with photon via hole, starts photon counter；S3: pipe is added by reagent, starting agent and exciting agent is added into reaction cup；S4: after a period of time, starting rotary drive makes through-hole and photon via hole on baffle misplace；S5: starting switch lid actuator makes top cover open measuring chamber opening, takes reaction cup away.Just start photon counter before starting agent and exciting agent is added and get through optical channel, anti-leak-stopping acquires photon, improves acquisition precision.
Description
Technical field
The present invention relates to a kind of luminous measurement methods of single reaction cup, belong to the field of medical instrument technology.
Background technique
The most commonly used diagnostic method of medical domain --- in-vitro diagnosis at present refers to body fluid, the row of acquisition human body
Object, secretion progress chemical component or chemical reaction analysis are let out, to judge human lesion.Such as chemiluminometry divides
Son diagnosis.These diagnostic methods are all made of automatically or semi-automatically instrument and are loaded, analysis, and provide diagnosis report.It is this kind of
Instrument has a functional unit --- luminous value detection unit (i.e. measuring chamber), measuring chamber be one can be with closed container knot
Structure measures collected number of photons by photon counter, finally carries out quantitative analysis after the interior reaction of measuring chamber shines.Existing skill
In art, the photon collection mouth of photon counter is open always, is opened when the top cover of measuring chamber and is placed or take away reaction cup
When, external light will enter in photon counter, to influence the service life and precision of photon counter.
Summary of the invention
The technical problem to be solved by the present invention is in order to overcome the problems referred above, providing a kind of higher single reaction of acquisition precision
The luminous measurement method of cup.
The technical solution adopted by the present invention to solve the technical problems is:
The present invention provides a kind of luminous measurement method of single reaction cup,
It is carried out in the luminous measuring chamber of single reaction cup, the luminous measuring chamber of single reaction cup includes:
Measuring chamber, top opening, inside have the accommodating chamber for accommodating reaction cup, and top is additionally provided with guide rail, and side has
Photon via hole is equipped with switch cover actuator；
Top cover is slidably arranged on the guide rail at the top of measuring chamber, can be under the driving of switch cover actuator along guide rail
Movement is additionally provided with examination to change between the position that closed measurement chamber is open and the position of opening measuring chamber opening on top cover
Pipe is added in agent, when top cover is in the position of closed measurement chamber opening, is held by the way that pipe is added and injects liquid and can flow into be located to reagent
It receives in the reaction cup of chamber；
Photon counter is arranged at measuring chamber photon via hole, for acquiring the luminous generated light of reagent in reaction cup
Son；
Shutter structure is arranged between photon counter and measuring chamber, can be closed or opened photon via hole, including peace
Dress rotary drive on a mounting board, the baffle with through-hole also form sliding slot on baffle, be driven for rotation part driving and
The trundle of rotation is arranged in sliding slot, when trundle rotates, by the cooperation with sliding slot, moves horizontally baffle, thus
The through-hole on baffle is set to be overlapped or misplace with photon via hole；
Sequence executes following steps:
S1: starting switch lid actuator makes top cover open measuring chamber opening, reaction cup is sent into the accommodating chamber of measuring chamber；
S2: starting switch lid actuator makes top closure measuring chamber be open, and start rotary drive make it is logical on baffle
Hole is overlapped with photon via hole, starts photon counter；
S3: pipe is added by reagent, starting agent and exciting agent is added into reaction cup；
S4: after a period of time, starting rotary drive makes through-hole and photon via hole on baffle misplace；
S5: starting switch lid actuator makes top cover open measuring chamber opening, takes reaction cup away.
Preferably, single reaction cup of the invention shines measurement method, and the time is 5 seconds in S4 step.
Preferably, the luminous measurement method of single reaction cup of the invention, photon counter is photomultiplier tube.
Preferably, the luminous measurement method of single reaction cup of the invention, when continuous acquisition photon obtains one section in S4 step
Interior total light yield.
Preferably, single reaction cup of the invention shines measurement method, and in S1 step, starting switch lid actuator beats top cover
Before opening measuring chamber opening, first determine that the through-hole and photon via hole on baffle are in coincidence status, if through-hole and photon on baffle
Via hole is in misaligned state, then cannot start switch lid actuator.
Preferably, the luminous measurement method of single reaction cup of the invention, the maximum one side of reaction cup area are directed at photon counting
Device.
Preferably, the luminous measurement method of single reaction cup of the invention, the lighting mirror of reaction cup central point and photon counter
The center point alignment of head.
The measurement method the beneficial effects of the present invention are: single reaction cup of the invention shines, by the design of shutter structure,
Before opening top cover, baffle movement is driven by rotary drive, so that through-hole and photon via hole on baffle be made to misplace, can be hidden
The camera lens for blocking photon counter prevents extraneous light from injecting photon counter, guarantees the service life and essence of photon counter
Degree.After top cover is closed, baffle movement is being driven by rotary drive, so that it is overlapped the through-hole on baffle with photon via hole,
In this way, photon counter can acquire the luminous quantity of substance in reaction cup.Just start photon before starting agent and exciting agent is added
Counter simultaneously gets through optical channel, and anti-leak-stopping acquires photon, improves acquisition precision.
Detailed description of the invention
Present invention will be further explained below with reference to the attached drawings and examples.
Fig. 1 is a kind of luminous measuring device (top cover closing) stereochemical structure of embodiment of analyzer single reaction cup of the present invention
Figure；
Fig. 2 is a kind of luminous measuring device (top cover opening) stereochemical structure of embodiment of analyzer single reaction cup of the present invention
Figure；
Fig. 3 is a kind of three-dimensional structure diagram of embodiment when shutter structure of the present invention is opened；
Fig. 4 is a kind of three-dimensional structure diagram of embodiment when shutter structure of the present invention is closed.
Appended drawing reference are as follows:
1- measuring chamber；12- guide rail；14- switch cover actuator；15- sliding block；16- sliding rail；2- top cover；21- reagent is added
Pipe；22- yoke；3- photon counter；40- mounting plate；401- photoelectric sensor；41- rotary drive；411- sliding slot；421-
Through-hole；42- baffle；422- trundle；5- position sensor.
Specific embodiment
In conjunction with the accompanying drawings, the present invention is further explained in detail.These attached drawings are simplified schematic diagram, only with
Illustration illustrates basic structure of the invention, therefore it only shows the composition relevant to the invention.
Embodiment 1
The present embodiment provides a kind of luminous measuring devices of single reaction cup, as shown in the figures 1 and 2: including:
Measuring chamber 1, top opening, inside have the accommodating chamber for accommodating reaction cup, and top is additionally provided with guide rail 12, side tool
There is photon via hole, switch cover actuator 14 is installed；
Top cover 2 is slidably arranged on the guide rail 12 at 1 top of measuring chamber, can be under the driving of switch cover actuator 14
It is moved along guide rail 12, to change between the position that closed measurement chamber 1 is open and the position that opening measuring chamber 1 is open, on top cover 2
It is additionally provided with reagent and pipe 21 is added, when top cover 2 is in the position of the opening of closed measurement chamber 1, injected by the way that pipe 21 is added to reagent
Liquid can flow into the reaction cup of accommodating chamber, and convenient for the dropwise addition of various reactants, luminous agent, switch cover actuator 14 is driven
The specific kind of drive that top cover 2 moves are as follows: be provided with the sliding rail 16 parallel with guide rail 12 at the top of measuring chamber 1, set on sliding rail 16
It is equipped with the sliding block 15 directly connecting with the drive rod of switch cover actuator 14, one end of top cover 2 is connect with sliding block 15, this layout
Mode can make 2 top of top cover without other components, and manipulator, various liquid lines is enabled to have enough spaces to be arranged；
Photon counter 3 (photomultiplier tube) is arranged at 1 photon via hole of measuring chamber, for acquiring reagent in reaction cup
Photon caused by shining；
Shutter structure is arranged between photon counter 3 and measuring chamber 1, can be closed or opened as shown in figure 3 or 4
Photon via hole, including the rotary drive 41 being installed on mounting plate 40, the baffle 42 with through-hole 421, on baffle 42 also at
Type has sliding slot 411, is arranged in sliding slot 411 by the trundle 422 for turning the driving of actuator 41 and rotating, when trundle 422 rotates,
By the cooperation with sliding slot 411, move horizontally baffle 42, so that the through-hole 421 on baffle 42 be made to be overlapped with photon via hole
(baffle 42 does not block photon) or dislocation (baffle 42 blocks photon).
By the design of shutter structure, before opening top cover 2, baffle 42 is driven to move by rotary drive 41, thus
So that through-hole 421 and photon via hole on baffle 42 is misplaced, the camera lens of photon counter 3 can be sheltered from, prevent extraneous light from penetrating
Enter photon counter 3, guarantees the service life and precision of photon counter.After top cover 2 is closed, driven by rotary drive 41
Dynamic baffle 42 moves, to make the through-hole 421 on baffle 42 be overlapped with photon via hole, in this way, photon counter can acquire instead
Answer the luminous quantity of substance in cup.
As further improving, also settable sensor, for incuding whether photon via hole is blocked by baffle 42, such as
Position sensor is set whether to incude baffle 42 positioned at the position for blocking photon via hole, once baffle 42 is not located at and blocks photon
When the position of via hole, then switch cover actuator 14 is prevented to open measuring chamber 1, to prevent photon counter 3 from being shone by extraneous light
It is mapped to and is damaged.Such as 40 side of mounting plate install photoelectric sensor 401, when the through-hole 421 on baffle 42 be pulled to
It when the position of photon via hole dislocation, then can be sensed by photoelectric sensor 401, be beaten at this point, switch cover actuator 14 can just start
Open top cover 2.
In order to facilitate manipulator to the hands free operation of reaction cup, enable reaction cup automatically into measuring chamber, this implementation
Example further includes stripper, and stripper includes a pair of of the yoke 22 arranged in parallel being arranged on 2 side of top cover, at top cover 2
When opening the position that measuring chamber 1 is open, yoke 22 is located above accommodating chamber.This be designed as adapt to specific structure manipulator and
It does, this specific structure manipulator has discharging blocking arm, and discharging blocking arm overlaps yoke 22, and manipulator, which continues decentralization, may make machine
Tool finger abandons reaction cup.1 top of measuring chamber be arranged position sensor 5 (photoelectric sensor transmitting terminal), top cover 2 be equipped with
The corresponding baffle of transmitting terminal.When top cover 2, which is closed, to be completed, transmitting terminal can sense baffle, so as to send to control section
The signal that top cover 2 is turned off, thus the operation of shutdown switch lid actuator 14, or continue other relevant actions.
Embodiment 2
The present embodiment provides a kind of luminous measurement methods of single reaction cup, in the luminous measuring chamber of single reaction cup of embodiment 1
It carries out；
Sequence executes following steps:
S1: starting switch lid actuator 14 makes top cover 2 open the opening of measuring chamber 1, and reaction cup is sent into the receiving of measuring chamber 1
It is intracavitary；
S2: starting switch lid actuator 14 makes 2 closed measurement chamber 1 of top cover be open, and starts rotary drive 41 and make baffle
Through-hole 421 on 42 is overlapped with photon via hole, starts photon counter 3；
S3: pipe 21 is added by reagent, starting agent and exciting agent is added into reaction cup；
S4: after a period of time, starting rotary drive 41 makes through-hole 421 and photon via hole on baffle 42 misplace；
S5: starting switch lid actuator 14 makes top cover 2 open the opening of measuring chamber 1, takes reaction cup away.
Preferably, the time is 5 seconds in S4 step.
Preferably, photon counter 3 is photomultiplier tube.
Preferably, continuous acquisition photon obtains the total light yield in a period of time in S4 step.
Preferably, in S1 step, start switch lid actuator 14 make top cover 2 open measuring chamber 1 be open before, first determine baffle
Through-hole 421 and photon via hole on 42 are in coincidence status, if the through-hole 421 on baffle 42 and photon via hole are in dislocation shape
State cannot then start switch lid actuator 14.
Preferably, the maximum one side alignment photon counter 3 of reaction cup area is prevented with acquiring photon as much as possible
Small error.
Preferably, the center point alignment of the daylighting camera lens of reaction cup central point and photon counter 3, i.e. height are concordant, light
Axis is directed at reaction cup central point.
The luminous measurement method of the single reaction cup of the present embodiment, just starts photon counter before starting agent and exciting agent is added
3 and optical channel is got through, anti-leak-stopping acquires photon, improves acquisition precision.In addition, continuous acquisition number of photons, obtained in a period of time
Total number of light photons, accuracy when can obtain than interim acquisition number of photons is higher.
Taking the above-mentioned ideal embodiment according to the present invention as inspiration, through the above description, relevant staff is complete
Various changes and amendments can be carried out without departing from the scope of the technological thought of the present invention' entirely.The technology of this invention
Property range is not limited to the contents of the specification, it is necessary to which the technical scope thereof is determined according to the scope of the claim.
Claims (6)
- The measurement method 1. a kind of single reaction cup shines, which is characterized in thatIt is carried out in the luminous measuring chamber of single reaction cup, the luminous measuring chamber of single reaction cup includes:Measuring chamber (1), top opening, inside have the accommodating chamber for accommodating reaction cup, and top is additionally provided with guide rail (12), side tool There is photon via hole, switch cover actuator (14) are installed；Top cover (2) is slidably arranged on the guide rail (12) at the top of measuring chamber (1), can be in the drive of switch cover actuator (14) It is moved under dynamic along guide rail (12), to become between the position that closed measurement chamber (1) is open and the position of opening measuring chamber (1) opening Change, reagent addition pipe (21) is additionally provided on top cover (2) and is passed through when top cover (2) is in the position of closed measurement chamber (1) opening Pipe (21) injection liquid, which is added, to reagent can flow into the reaction cup of accommodating chamber；Photon counter (3) is arranged at measuring chamber (1) photon via hole, luminous generated for acquiring reagent in reaction cup Photon；Shutter structure is arranged between photon counter (3) and measuring chamber (1), can be closed or opened photon via hole, including The rotary drive (41) being mounted on mounting plate (40), the baffle (42) with through-hole (421), baffle also form on (42) Sliding slot (411), trundle (422) setting for being driven for rotation part (41) driving and rotating is interior in sliding slot (411), trundle (422) when rotating, by the cooperation with sliding slot (411), move horizontally baffle (42), to make the through-hole on baffle (42) (421) it is overlapped or misplaces with photon via hole；Sequence executes following steps:S1: starting switch lid actuator (14) makes top cover (2) to open measuring chamber (1) opening, and reaction cup is sent into measuring chamber (1) In accommodating chamber；S2: starting switch lid actuator (14) makes top cover (2) closed measurement chamber (1) be open, and starts rotary drive (41) and make Through-hole (421) on baffle (42) is overlapped with photon via hole, is started photon counter (3)；S3: pipe (21) is added by reagent, starting agent and exciting agent is added into reaction cup；S4: after a period of time, starting rotary drive (41) makes through-hole (421) and photon via hole mistake on baffle (42) Position；S5: starting switch lid actuator (14) makes top cover (2) to open measuring chamber (1) opening, takes reaction cup away.
- The measurement method 2. single reaction cup according to claim 1 shines, which is characterized in that the time is 5 seconds in S4 step.
- The measurement method 3. single reaction cup according to claim 1 shines, which is characterized in that photon counter (3) is photoelectricity Multiplier tube.
- The measurement method 4. single reaction cup according to claim 3 shines, which is characterized in that the continuous acquisition light in S4 step Son obtains the total light yield in a period of time.
- The measurement method 5. single reaction cup according to claim 1-4 shines, which is characterized in that reaction cup area is most Big one side is directed at photon counter (3).
- The measurement method 6. single reaction cup according to claim 5 shines, which is characterized in that reaction cup central point and photon meter The center point alignment of the daylighting camera lens of number device (3).
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| CN201710507536.6A CN107271433B (en) | 2017-06-28 | 2017-06-28 | A kind of luminous measurement method of single reaction cup |
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| CN201710507536.6A CN107271433B (en) | 2017-06-28 | 2017-06-28 | A kind of luminous measurement method of single reaction cup |
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| CN107271433B true CN107271433B (en) | 2019-07-30 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110412019A (en) * | 2018-04-28 | 2019-11-05 | 上海索昕生物科技有限公司 | A kind of detection device and full-automatic light-induced chemiluminescent detector |
| CN110412258A (en) * | 2018-04-28 | 2019-11-05 | 上海索昕生物科技有限公司 | Full-automatic light-induced chemiluminescent detector |
| CN109374258A (en) * | 2018-09-26 | 2019-02-22 | 苏州长光华医生物医学工程有限公司 | A kind of photomultiplier tube test structure and luminous value measurement method |
| CN112630215B (en) * | 2020-11-30 | 2022-02-18 | 苏州长光华医生物医学工程有限公司 | Multi-reaction-cup continuous measurement luminescence measurement device and luminescence measurement method |
| CN113011549A (en) * | 2021-02-20 | 2021-06-22 | 重庆博奥新景医学科技有限公司 | Self-adaptive measurement and calculation method applied to luminescence value of chemiluminescence analyzer |
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