CN109374258A - A kind of photomultiplier tube test structure and luminous value measurement method - Google Patents
A kind of photomultiplier tube test structure and luminous value measurement method Download PDFInfo
- Publication number
- CN109374258A CN109374258A CN201811124660.5A CN201811124660A CN109374258A CN 109374258 A CN109374258 A CN 109374258A CN 201811124660 A CN201811124660 A CN 201811124660A CN 109374258 A CN109374258 A CN 109374258A
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- CN
- China
- Prior art keywords
- photomultiplier tube
- reaction cup
- light
- cavity
- measurement cavity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 238000012360 testing method Methods 0.000 title claims abstract description 21
- 238000000691 measurement method Methods 0.000 title claims description 5
- 238000005259 measurement Methods 0.000 claims abstract description 34
- 230000003287 optical effect Effects 0.000 claims description 13
- 239000000758 substrate Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 238000007689 inspection Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims 1
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 abstract description 3
- 239000002184 metal Substances 0.000 abstract description 3
- 230000002093 peripheral effect Effects 0.000 abstract description 3
- 238000010586 diagram Methods 0.000 description 4
- 239000012141 concentrate Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- JUJWROOIHBZHMG-UHFFFAOYSA-N pyridine Substances C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 1
- -1 pyridine ester Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01M—TESTING STATIC OR DYNAMIC BALANCE OF MACHINES OR STRUCTURES; TESTING OF STRUCTURES OR APPARATUS, NOT OTHERWISE PROVIDED FOR
- G01M11/00—Testing of optical apparatus; Testing structures by optical methods not otherwise provided for
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J1/00—Photometry, e.g. photographic exposure meter
- G01J1/42—Photometry, e.g. photographic exposure meter using electric radiation detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J1/00—Photometry, e.g. photographic exposure meter
- G01J1/42—Photometry, e.g. photographic exposure meter using electric radiation detectors
- G01J1/44—Electric circuits
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J1/00—Photometry, e.g. photographic exposure meter
- G01J1/42—Photometry, e.g. photographic exposure meter using electric radiation detectors
- G01J1/44—Electric circuits
- G01J2001/4413—Type
- G01J2001/442—Single-photon detection or photon counting
Abstract
The present invention relates to a kind of photomultiplier tubes to test structure, and the measuring chamber side is provided with the fixing seat, and the photomultiplier tube is set in the fixing seat, and the photomultiplier tube is connected with photon counter；There is measurement cavity in the measuring chamber, one end of the measurement cavity is hemispherical, the other end of the measurement cavity is connected on the photomultiplier tube, the connection end of the measurement cavity and photomultiplier tube is equipped with shutter assembly, inserted with reaction cup in the measurement cavity, the reaction cup is not contacted with the measurement cavity inner wall, and the reaction cup is set to the focal point of the domed ends of the measurement cavity.In photomultiplier tube test structure provided by the invention, reaction cup peripheral region is not directly fitted with cavity face, and the light for avoiding reaction cup from issuing directly is sponged by non-ferrous metal, causes light loss, and the photon that the one side of reaction cup back to photomultiplier tube issues can be reflected, reduce light loss.
Description
Technical field
The present invention relates to medical instruments field more particularly to a kind of photomultiplier tube test structure and luminous value measurement sides
Method.
Background technique
Measuring chamber is the luminous value for measuring bifurcation pyridine ester in reaction cup in chemiluminescence system, and system is by shining
Value determines the concentration value of sample.In Full-automatic chemiluminescence equipment, the chemical luminescence for liquid in reaction cup enters measurement chamber
Before body, it is initially injected substrate A, measuring chamber cavity is moved into after suitably shaking up, substrate B is reinjected, has collect very much at this time
In light issue, after being acquired by photomultiplier tube by photon counter be passed to computer.The reaction pattern can concentrated very much
Moment (in 30ms) issues a large amount of photons, these photons can lose one since the design of measuring chamber cavity body structure is unreasonable
Point, it is read after then being collected by multiplier tube by counter.Counter is the reading number of photons by way of pulse signal, but due to
The number of photons that moment issues is too many, and the overlapping of pulsing period causes the photon ratio actual value read relatively low, this phenomenon exists
Luminous value is more than to show particularly evident after 1000W, this will result in survey of light inspection liquid of different luminous values from low value to high level
The not high defect of the magnitude linearity.
Summary of the invention
The technical solution adopted by the present invention to solve the technical problems is: a kind of photomultiplier tube test structure comprising:
Measuring chamber, fixing seat and photomultiplier tube, the measuring chamber side are provided with the fixing seat, and the photomultiplier tube is set to
In the fixing seat, and the photomultiplier tube is connected with photon counter；
The measuring chamber is interior to have measurement cavity, and one end of the measurement cavity is hemispherical, described to measure the another of cavity
One end is connected on the photomultiplier tube, and the connection end of the measurement cavity and photomultiplier tube is equipped with shutter assembly, institute
It states in measurement cavity inserted with reaction cup, the reaction cup is not contacted with the measurement cavity inner wall, and the reaction cup is arranged
In the focal point of the domed ends of the measurement cavity.
Further improvement is that the fixing seat is equipped with light-measuring hole, and the light-measuring hole is equipped with optical filter.
Further improvement is that the photomultiplier tube side of the light-measuring hole is equipped with boss, and is additionally provided on the light-measuring hole solid
Determine ring, the fixed ring is used to for optical filter being fixed on the boss of the light-measuring hole.
Further improvement, which is that the fixed ring is threadably secured, to be connected on the light-measuring hole.
The present invention also provides a kind of luminous value measurements based on photomultiplier tube described in any of the above embodiments test structure
Method comprising following steps:
The reaction cup for being injected with light inspection liquid and the first substrate is put into the cavity of measuring chamber by A, and reaction cup is located at cavity
Hemispherical end focal point；
B opens shutter assembly, and the second substrate is injected into reaction cup；
C photomultiplier tube collects light of the reaction cup towards the photomultiplier tube side photon issued and hemispherical end reflection
Son；
D photon counter reads number of photons.
Further improvement is that light-measuring hole is equipped in the fixing seat of photomultiplier tube, and the light-measuring hole is equipped with filter ratio
Example is the optical filter of Z%, and when calculating luminous value, the number of photons that photon counter is read is multiplied by 1/Z.
The beneficial effects of the present invention are:
In photomultiplier tube test structure provided by the invention, reaction cup peripheral region is not directly fitted with cavity face, is kept away
The light for exempting from reaction cup sending is directly sponged by non-ferrous metal, causes light loss.
In photomultiplier tube test structure provided by the invention, the cavity body structure of measuring chamber placing response cup is designed to bottom surface
Spherical structure, and be in reaction cup at spherical 1/2 radius (i.e. spherical surface focal point), so that reaction cup is back to photomultiplier tube
The photon issued on one side is reflected back in the form of directional light after spheric reflection, is connect as much as possible by photomultiplier tube
It receives, reduces light loss.
In luminous value measurement method provided by the invention, the optical filter for increasing Z% before the camera lens of photomultiplier tube (is filtered
Fall the number of photons of Z%) so that the proportional reduction of the photon received by photomultiplier tube, and then reduce and even avoid photon meter
Since light intensity excessively concentrates the pulse signal cycle overlapping phenomenon of appearance when number device reads pulse signal；Meanwhile calculating photon
When number, output test result after the number of photons that photon counter is read is corrected multiplied by 1/Z, the linearity as a result is sent out in 2000W
It can achieve the linear dependence with 0.99 or more theoretical value within light value.
Detailed description of the invention
Present invention will be further explained below with reference to the attached drawings and examples.
Fig. 1 is the structural schematic diagram of photomultiplier tube test structure of the invention；
Fig. 2 is measurement cavity body structure schematic diagram of the invention；
Fig. 3 is fixed seat structure schematic diagram of the invention.
Specific embodiment
In conjunction with the accompanying drawings, the present invention is further explained in detail.These attached drawings are simplified schematic diagram, only with
Illustration illustrates basic structure of the invention, therefore it only shows the composition relevant to the invention.
In the description of invention, it is to be understood that term " center ", " longitudinal direction ", " transverse direction ", " length ", " width ",
" thickness ", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom" "inner", "outside", " up time
The orientation or positional relationship of the instructions such as needle ", " counterclockwise " is to be based on the orientation or positional relationship shown in the drawings, and is merely for convenience of
Description invention and simplified description, rather than the device or element of indication or suggestion meaning must have a particular orientation, with specific
Orientation construction and operation, therefore should not be understood as the limitation to invention.
In addition, term " first ", " second " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance
Or implicitly indicate the quantity of indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or
Implicitly include one or more of the features.In the description of invention, the meaning of " plurality " is two or more, remove
It is non-separately to have clearly specific restriction.
In invention unless specifically defined or limited otherwise, the arts such as term " installation ", " connected ", " connection ", " fixation "
Language shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can be machinery
Connection, is also possible to be electrically connected；It can be directly connected, two elements can also be can be indirectly connected through an intermediary
Internal connection.For the ordinary skill in the art, it can understand above-mentioned term in invention as the case may be
Concrete meaning.
As depicted in figs. 1 and 2, the present invention provides a kind of photomultiplier tubes to test structure comprising: measuring chamber 1 is fixed
Seat 2 and photomultiplier tube 3,1 side of measuring chamber are fixedly connected with the fixing seat 2, and the photomultiplier tube 3 is installed on institute
It states in fixing seat 2, and the photomultiplier tube 3 is connected with photon counter；
There is measurement cavity 11, one end of the measurement cavity 11 is outstanding to outside measurement cavity in the measuring chamber 1
The other end of hemispherical, the measurement cavity 11 is connected on the photomultiplier tube 3, the measurement cavity 11 and photomultiplier transit
The connection end 13 of pipe 3 be equipped with shutter assembly 4, the shutter assembly be used for control measurement cavity and photomultiplier tube connection and
It is no, inserted with reaction cup 100, the direction of insertion of the reaction cup 100, perpendicular to the measurement cavity in the measurement cavity 11
11 to photomultiplier tube 3 extending direction, the reaction cup 100 is not contacted with 11 inner wall of measurement cavity, and the reaction
Cup 100 is set to the focal point of the domed ends 12 of the measurement cavity 11.
As shown in figure 3, being further improved is that the fixing seat 2 is equipped with light-measuring hole 21, the light-measuring hole 21 is equipped with filter
Mating plate 5, the optical filter 5 are used to filter out the number of photons of the Z% of reaction cup sending.
Further improvement is that the photomultiplier tube side of the light-measuring hole 21 is equipped with boss 22, and on the light-measuring hole 21 also
Equipped with fixed ring 6, the fixed ring 6 is pressed in the edge of optical filter 5, for optical filter 5 to be fixed on to the institute of the light-measuring hole 21
It states on boss 22, meanwhile, annular fixed ring 6 will not block light-measuring hole 21.
Further improvement, which is that the fixed ring 6 is threadably secured, to be connected on the light-measuring hole 21.
In photomultiplier tube test structure provided by the invention, reaction cup peripheral region is not directly fitted with cavity face, is kept away
The light for exempting from reaction cup sending is directly sponged by non-ferrous metal, causes light loss.
In photomultiplier tube test structure provided by the invention, the cavity body structure of measuring chamber placing response cup is designed to bottom surface
Spherical structure, and be in reaction cup at spherical 1/2 radius (i.e. spherical surface focal point), so that reaction cup is back to photomultiplier tube
The photon issued on one side is reflected back in the form of directional light after spheric reflection, is connect as much as possible by photomultiplier tube
It receives, reduces light loss.
The present invention also provides a kind of luminous value measurements based on photomultiplier tube described in any of the above embodiments test structure
Method comprising following steps:
The reaction cup for being injected with light inspection liquid and the first substrate (substrate A) is put into the cavity of measuring chamber by A, and reaction cup position
In the focal point of the hemispherical end of cavity；
B opens shutter assembly, and the second substrate (substrate B) is injected into reaction cup；
C photomultiplier tube collects light of the reaction cup towards the photomultiplier tube side photon issued and hemispherical end reflection
Son；
D photon counter reads number of photons.
Further improvement is that light-measuring hole is equipped in the fixing seat of photomultiplier tube, and the light-measuring hole is equipped with filter ratio
Example is the optical filter of Z%, and when calculating luminous value, the number of photons that photon counter is read is multiplied by 1/Z.
In luminous value measurement method provided by the invention, the optical filter for increasing Z% before the camera lens of photomultiplier tube (is filtered
Fall the number of photons of Z%) so that the proportional reduction of the photon received by photomultiplier tube, and then reduce and even avoid photon meter
Since light intensity excessively concentrates the pulse signal cycle overlapping phenomenon of appearance when number device reads pulse signal；Meanwhile calculating photon
When number, output test result after the number of photons that photon counter is read is corrected multiplied by 1/Z, the linearity as a result is sent out in 2000W
It can achieve the linear dependence with 0.99 or more theoretical value within light value.
Taking the above-mentioned ideal embodiment according to the present invention as inspiration, through the above description, relevant staff is complete
Various changes and amendments can be carried out without departing from the scope of the technological thought of the present invention' entirely.The technology of this invention
Property range is not limited to the contents of the specification, it is necessary to which the technical scope thereof is determined according to the scope of the claim.
Claims (6)
1. a kind of photomultiplier tube tests structure characterized by comprising measuring chamber, fixing seat and photomultiplier tube, the survey
Amount room side is provided with the fixing seat, and the photomultiplier tube is set in the fixing seat, and the photomultiplier tube connects
It is connected to photon counter；
There is measurement cavity, one end of the measurement cavity is hemispherical, the other end of the measurement cavity in the measuring chamber
It is connected on the photomultiplier tube, the connection end of the measurement cavity and photomultiplier tube is equipped with shutter assembly, the survey
It measures in cavity inserted with reaction cup, the reaction cup is not contacted with the measurement cavity inner wall, and the reaction cup is set to institute
State the focal point of the domed ends of measurement cavity.
2. photomultiplier tube according to claim 1 tests structure, which is characterized in that the fixing seat, which is equipped with, surveys light
Hole, the light-measuring hole are equipped with optical filter.
3. photomultiplier tube according to claim 2 tests structure, which is characterized in that the photomultiplier tube of the light-measuring hole
Side is equipped with boss, and fixed ring is additionally provided on the light-measuring hole, and the fixed ring is used to optical filter being fixed on the light-measuring hole
The boss on.
4. photomultiplier tube according to claim 3 tests structure, which is characterized in that the fixed ring is threadably secured
It is connected on the light-measuring hole.
5. a kind of luminous value measurement method based on photomultiplier tube of any of claims 1-4 test structure,
It is characterized in that, includes the following steps:
The reaction cup for being injected with light inspection liquid and the first substrate is put into the cavity of measuring chamber by A, and reaction cup is located at the half of cavity
The focal point of spheric end；
B opens shutter assembly, and the second substrate is injected into reaction cup；
C photomultiplier tube collects photon of the reaction cup towards the photomultiplier tube side photon issued and hemispherical end reflection；
D photon counter reads number of photons.
6. according to the method described in claim 5, it is characterized in that, being equipped with light-measuring hole, institute in the fixing seat of photomultiplier tube
The optical filter that light-measuring hole is Z% equipped with optical filtering ratio is stated, when calculating luminous value, the number of photons that photon counter is read is multiplied
With 1/Z.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811124660.5A CN109374258A (en) | 2018-09-26 | 2018-09-26 | A kind of photomultiplier tube test structure and luminous value measurement method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811124660.5A CN109374258A (en) | 2018-09-26 | 2018-09-26 | A kind of photomultiplier tube test structure and luminous value measurement method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109374258A true CN109374258A (en) | 2019-02-22 |
Family
ID=65402645
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811124660.5A Pending CN109374258A (en) | 2018-09-26 | 2018-09-26 | A kind of photomultiplier tube test structure and luminous value measurement method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109374258A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030148536A1 (en) * | 2002-02-04 | 2003-08-07 | Chi-Wang Liang | Chemiluminescence analyzer |
| JP2004163307A (en) * | 2002-11-14 | 2004-06-10 | Matsushita Electric Ind Co Ltd | Radiation measurement instrument |
| US20040241777A1 (en) * | 2001-04-12 | 2004-12-02 | Yoshihito Suzuki | Luminescent Reaction Measurement Device |
| CN101980006A (en) * | 2010-09-17 | 2011-02-23 | 深圳市新产业生物医学工程有限公司 | Chemiluminescence detection device |
| CN104089939A (en) * | 2014-07-21 | 2014-10-08 | 深圳市奥特库贝科技有限公司 | Novel fluorescence determination device and method |
| CN104568755A (en) * | 2014-12-29 | 2015-04-29 | 苏州长光华医生物医学工程有限公司 | Optical fiber conduction device in chemical light emission measuring chamber |
| CN107167427A (en) * | 2017-06-28 | 2017-09-15 | 苏州长光华医生物医学工程有限公司 | A kind of luminous measuring chamber of single reaction cup |
| CN107271433A (en) * | 2017-06-28 | 2017-10-20 | 苏州长光华医生物医学工程有限公司 | A kind of luminous measuring method of single reaction cup |
| CN209148877U (en) * | 2018-09-26 | 2019-07-23 | 苏州长光华医生物医学工程有限公司 | A kind of photomultiplier tube test structure |
-
2018
- 2018-09-26 CN CN201811124660.5A patent/CN109374258A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040241777A1 (en) * | 2001-04-12 | 2004-12-02 | Yoshihito Suzuki | Luminescent Reaction Measurement Device |
| US20030148536A1 (en) * | 2002-02-04 | 2003-08-07 | Chi-Wang Liang | Chemiluminescence analyzer |
| JP2004163307A (en) * | 2002-11-14 | 2004-06-10 | Matsushita Electric Ind Co Ltd | Radiation measurement instrument |
| CN101980006A (en) * | 2010-09-17 | 2011-02-23 | 深圳市新产业生物医学工程有限公司 | Chemiluminescence detection device |
| CN104089939A (en) * | 2014-07-21 | 2014-10-08 | 深圳市奥特库贝科技有限公司 | Novel fluorescence determination device and method |
| CN104568755A (en) * | 2014-12-29 | 2015-04-29 | 苏州长光华医生物医学工程有限公司 | Optical fiber conduction device in chemical light emission measuring chamber |
| CN107167427A (en) * | 2017-06-28 | 2017-09-15 | 苏州长光华医生物医学工程有限公司 | A kind of luminous measuring chamber of single reaction cup |
| CN107271433A (en) * | 2017-06-28 | 2017-10-20 | 苏州长光华医生物医学工程有限公司 | A kind of luminous measuring method of single reaction cup |
| CN209148877U (en) * | 2018-09-26 | 2019-07-23 | 苏州长光华医生物医学工程有限公司 | A kind of photomultiplier tube test structure |
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