CN107271433A - A kind of luminous measuring method of single reaction cup - Google Patents
A kind of luminous measuring method of single reaction cup Download PDFInfo
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- CN107271433A CN107271433A CN201710507536.6A CN201710507536A CN107271433A CN 107271433 A CN107271433 A CN 107271433A CN 201710507536 A CN201710507536 A CN 201710507536A CN 107271433 A CN107271433 A CN 107271433A
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- reaction cup
- photon
- measuring chamber
- baffle plate
- luminous
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- Chemical Kinetics & Catalysis (AREA)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention relates to a kind of luminous measuring method of single reaction cup, following steps：S1：Starting switch lid actuator makes top cover open measuring chamber opening, in the accommodating chamber that reaction cup is sent into measuring chamber；S2：Starting switch lid actuator makes top closure measuring chamber opening, and starting rotary drive makes the through hole on baffle plate be overlapped with photon via, starts photon counter；S3：Pipe is added by reagent startup agent and exciting agent are added into reaction cup；S4：The through hole on baffle plate is set to be misplaced with photon via through after a period of time, starting rotary drive；S5：Starting switch lid actuator makes top cover open measuring chamber opening, takes reaction cup away.Just start photon counter before startup agent and exciting agent is added and get through optical channel, anti-leak-stopping gathers photon, improves acquisition precision.
Description
Technical field
The present invention relates to a kind of luminous measuring method of single reaction cup, belong to technical field of medical instruments.
Background technology
The most commonly used diagnostic method of current medical domain --- in-vitro diagnosis, refers to gather the body fluid of human body, row
Let out thing, secretion and carry out chemical composition or chemical reaction analysis, so as to judge human lesion.As chemiluminometry, point
Son diagnosis.These diagnostic methods, are loaded using automatically or semi-automatically instrument, are analyzed, and provide diagnosis report.It is this kind of
Instrument has One function unit --- and luminous value detection unit (i.e. measuring chamber), measuring chamber is the container that can a be closed knot
After reaction is luminous in structure, measuring chamber, the number of photons collected by photon counter measurement finally carries out quantitative analysis.Existing skill
In art, the photon collection mouthful of photon counter is always what is opened, is opened when the top cover of measuring chamber and places or take away reaction cup
When, outside light will enter in photon counter, so as to influence the service life and precision of photon counter.
The content of the invention
The technical problem to be solved in the present invention is：To overcome above mentioned problem, there is provided a kind of higher single reaction of acquisition precision
The luminous measuring method of cup.
The technical solution adopted for the present invention to solve the technical problems is：
The present invention provides a kind of single reaction cup luminous measuring method,
Carried out in the luminous measuring chamber of single reaction cup, the luminous measuring chamber of single reaction cup includes：
Measuring chamber, open top, inside has the accommodating chamber for accommodating reaction cup, and top is additionally provided with guide rail, had sideways
Photon via, is provided with switch cover actuator；
Top cover, is slidably arranged on the guide rail at the top of measuring chamber, can be under the driving of switch cover actuator along guide rail
Motion, to change between the position of closed measurement chamber opening and the position of opening measuring chamber opening, examination is additionally provided with top cover
Agent, which is added, manages, when top cover is in the position of closed measurement chamber opening, can be flowed into positioned at appearance by adding pipe injection liquid to reagent
Receive in the reaction cup of chamber；
Photon counter, is arranged at measuring chamber photon via, for gathering the luminous produced light of reagent in reaction cup
Son；
Shutter structure, is arranged between photon counter and measuring chamber, can close or open photon via, including peace
Also form chute, part driving driven for rotation on dress rotary drive on a mounting board, the baffle plate with through hole, baffle plate and
The trundle of rotation is arranged in chute, when trundle is rotated, by the cooperation with chute, moves horizontally baffle plate, so that
The through hole on baffle plate is set to overlap or misplace with photon via；
Order performs following steps：
S1：Starting switch lid actuator makes top cover open measuring chamber opening, in the accommodating chamber that reaction cup is sent into measuring chamber；
S2：Starting switch lid actuator makes top closure measuring chamber opening, and start rotary drive make it is logical on baffle plate
Hole is overlapped with photon via, starts photon counter；
S3：Pipe is added by reagent startup agent and exciting agent are added into reaction cup；
S4：The through hole on baffle plate is set to be misplaced with photon via through after a period of time, starting rotary drive；
S5：Starting switch lid actuator makes top cover open measuring chamber opening, takes reaction cup away.
Preferably, the time is 5 seconds in the luminous measuring method of single reaction of the invention cup, S4 steps.
Preferably, the luminous measuring method of single reaction of the invention cup, photon counter is photomultiplier.
Preferably, the luminous measuring method of single reaction of the invention cup, when continuous acquisition photon obtains one section in S4 steps
Interior total light yield.
Preferably, in the luminous measuring method of single reaction of the invention cup, S1 steps, starting switch lid actuator beats top cover
Open before measuring chamber opening, first determine that the through hole on baffle plate is in coincidence status with photon via, if through hole and photon on baffle plate
Via is in misaligned state, then is unable to starting switch lid actuator.
Preferably, the luminous measuring method of single reaction of the invention cup, the maximum one side alignment photon counting of reaction cup area
Device.
Preferably, the lighting mirror of the luminous measuring method of single reaction of the invention cup, reaction cup central point and photon counter
The central point alignment of head.
The beneficial effects of the invention are as follows：The luminous measuring method of single reaction cup of the present invention, by the design of shutter structure,
Open before top cover, drive baffle plate to move by rotary drive, so that the through hole on baffle plate misplaces with photon via, you can hide
The camera lens of photon counter is blocked, prevents extraneous light from injecting photon counter, it is ensured that the service life and essence of photon counter
Degree.After top cover is closed, baffle plate is being driven to move by rotary drive, so that the through hole on baffle plate is overlapped with photon via,
In this way, photon counter can gather the luminous quantity of material in reaction cup.Just start photon before startup agent and exciting agent is added
Counter simultaneously gets through optical channel, and anti-leak-stopping gathers photon, improves acquisition precision.
Brief description of the drawings
The present invention is further described with reference to the accompanying drawings and examples.
Fig. 1 is analyzer single reaction cup a kind of stereochemical structure of embodiment of luminous measurement apparatus (top cover closing) of the present invention
Figure；
Fig. 2 is analyzer single reaction cup a kind of stereochemical structure of embodiment of luminous measurement apparatus (top cover opening) of the present invention
Figure；
Fig. 3 is a kind of three-dimensional structure diagram of embodiment when shutter structure of the present invention is opened；
Fig. 4 is a kind of three-dimensional structure diagram of embodiment when shutter structure of the present invention is closed.
Reference is：
1- measuring chambers；12- guide rails；14- switch cover actuators；15- sliding blocks；16- slide rails；2- top covers；21- reagents are added
Pipe；22- yokes；3- photon counters；40- installing plates；401- photoelectric sensors；41- rotary drives；411- chutes；421-
Through hole；42- baffle plates；422- trundles；5- position sensors.
Embodiment
In conjunction with the accompanying drawings, the present invention is further explained in detail.These accompanying drawings are simplified schematic diagram, only with
Illustration illustrates the basic structure of the present invention, therefore it only shows the composition relevant with the present invention.
Embodiment 1
The present embodiment provides a kind of single reaction cup luminous measurement apparatus, as shown in the figures 1 and 2：Including：
Measuring chamber 1, open top, inside has the accommodating chamber for accommodating reaction cup, and top is additionally provided with guide rail 12, and side has
There is photon via, switch cover actuator 14 is installed；
, can be under the driving of switch cover actuator 14 on top cover 2, the guide rail 12 for being slidably arranged in the top of measuring chamber 1
Moved along guide rail 12, to change between the position that the position and opening measuring chamber 1 that closed measurement chamber 1 is open are open, on top cover 2
It is additionally provided with reagent and adds pipe 21, when top cover 2 is in the position that closed measurement chamber 1 is open, is injected by adding pipe 21 to reagent
Liquid can be flowed into the reaction cup of accommodating chamber, be easy to the dropwise addition of various reactants, luminous agent, and switch cover actuator 14 drives
Top cover 2 move the specific kind of drive be：The top of measuring chamber 1, which is provided with the slide rail 16 parallel with guide rail 12, slide rail 16, to be set
The sliding block 15 being directly connected with the drive link of switch cover actuator 14 is equipped with, one end of top cover 2 is connected with sliding block 15, this layout
Mode can make the top of top cover 2 without other parts so that manipulator, various liquid lines can have enough spaces to be arranged；
Photon counter 3 (photomultiplier), is arranged at the photon via of measuring chamber 1, for gathering reagent in reaction cup
Light produced photon；
Shutter structure, as shown in figure 3 or 4, is arranged between photon counter 3 and measuring chamber 1, can close or open
On photon via, including the rotary drive 41 being installed on installing plate 40, the baffle plate 42 with through hole 421, baffle plate 42 also into
Type has chute 411, is arranged on by the trundle 422 that actuator 41 drives and rotate is turned in chute 411, when trundle 422 is rotated,
By the cooperation with chute 411, move horizontally baffle plate 42, so that the through hole 421 on baffle plate 42 is overlapped with photon via
(baffle plate 42 does not block photon) or dislocation (baffle plate 42 blocks photon).
By the design of shutter structure, before top cover 2 is opened, baffle plate 42 is driven to move by rotary drive 41, so that
The through hole 421 on baffle plate 42 is set to be misplaced with photon via, you can to shelter from the camera lens of photon counter 3, prevent extraneous light from penetrating
Enter photon counter 3, it is ensured that the service life and precision of photon counter.After top cover 2 is closed, driven by rotary drive 41
Dynamic baffle plate 42 is moved, so that the through hole 421 on baffle plate 42 is overlapped with photon via, in this way, photon counter can be gathered instead
Answer the luminous quantity of material in cup.
As further improving, sensor also can be set, for sensing whether photon via is blocked by baffle plate 42, such as
Whether set location sensor, which is located at sensing baffle plate 42, is blocked the position of photon via, once baffle plate 42 is not located at blocking photon
During the position of via, then switch cover actuator 14 is prevented to open measuring chamber 1, to prevent photon counter 3 from being shone by extraneous light
It is mapped to and is damaged.Such as the side of installing plate 40 install photoelectric sensor 401, when the through hole 421 on baffle plate 42 be pulled to
During the position of photon via dislocation, then it can be sensed by photoelectric sensor 401, now, switch cover actuator 14, which can just start, to be beaten
Open top cover 2.
Hands free operation of the manipulator to reaction cup for convenience so that reaction cup can be automatically into measuring chamber, this implementation
Example also includes stripper, and stripper is included at a pair of the yokes 22 arranged in parallel being arranged on the side of top cover 2, top cover 2
When the position that measuring chamber 1 is open is opened, yoke 22 is located above accommodating chamber.This be designed as adapt to specific structure manipulator and
Do, this specific structure manipulator has discharging blocking arm, discharging blocking arm overlap joint yoke 22, manipulator continues decentralization and may be such that machine
Tool finger abandons reaction cup.The top of measuring chamber 1 set position sensor 5 (photoelectric sensor transmitting terminal), top cover 2 be provided with
The corresponding catch of transmitting terminal.When top cover 2, which is closed, to be completed, transmitting terminal can sense catch, so as to be sent to control section
The signal that top cover 2 is turned off, so that the operation of shutdown switch lid actuator 14, or continue other relevant actions.
Embodiment 2
The present embodiment provides a kind of single reaction cup luminous measuring method, in the luminous measuring chamber of single reaction cup of embodiment 1
Carry out；
Order performs following steps：
S1：Starting switch lid actuator 14 makes the opening measuring chamber 1 of top cover 2 be open, and reaction cup is sent into the receiving of measuring chamber 1
Intracavitary；
S2：Starting switch lid actuator 14 makes the closed measurement chamber 1 of top cover 2 be open, and starts rotary drive 41 and make baffle plate
Through hole 421 on 42 is overlapped with photon via, starts photon counter 3；
S3：Pipe 21 is added by reagent startup agent and exciting agent are added into reaction cup；
S4：The through hole 421 on baffle plate 42 is set to be misplaced with photon via through after a period of time, starting rotary drive 41；
S5：Starting switch lid actuator 14 makes the opening measuring chamber 1 of top cover 2 be open, and takes reaction cup away.
Preferably, the time is 5 seconds in S4 steps.
Preferably, photon counter 3 is photomultiplier.
Preferably, continuous acquisition photon obtains the total light yield in a period of time in S4 steps.
Preferably, in S1 steps, starting switch lid actuator 14 makes top cover 2 open before the opening of measuring chamber 1, first determines baffle plate
Through hole 421 on 42 is in coincidence status with photon via, if the through hole 421 on baffle plate 42 is in dislocation shape with photon via
State, then be unable to starting switch lid actuator 14.
Preferably, the maximum one side alignment photon counter 3 of reaction cup area, to gather photon as much as possible, is prevented
Small error.
Preferably, reaction cup central point aligns with the central point of the daylighting camera lens of photon counter 3, i.e., height is concordant, light
Axle is directed at reaction cup central point.
The luminous measuring method of single reaction cup of the present embodiment, just starts photon counter before startup agent and exciting agent is added
3 and optical channel is got through, anti-leak-stopping gathers photon, improve acquisition precision.In addition, continuous acquisition number of photons, was obtained in a period of time
Total number of light photons, the accuracy that can be obtained during than interim acquisition number of photons is higher.
Using the above-mentioned desirable embodiment according to the present invention as enlightenment, by above-mentioned description, relevant staff is complete
Various changes and amendments can be carried out without departing from the scope of the technological thought of the present invention' entirely.The technology of this invention
Property scope is not limited to the content on specification, it is necessary to its technical scope is determined according to right.
Claims (7)
1. a kind of luminous measuring method of single reaction cup, it is characterised in that
Carried out in the luminous measuring chamber of single reaction cup, the luminous measuring chamber of single reaction cup includes：
Measuring chamber (1), open top, inside has the accommodating chamber for accommodating reaction cup, and top is additionally provided with guide rail (12), and side has
There is photon via, switch cover actuator (14) is installed；
Top cover (2), is slidably arranged on the guide rail (12) at the top of measuring chamber (1), can switch cover actuator (14) drive
Moved under dynamic along guide rail (12), with the anaplasia of the position being open in closed measurement chamber (1) and the position for opening measuring chamber (1) opening
Change, reagent is additionally provided with top cover (2) and adds pipe (21), when top cover (2) is in the position that closed measurement chamber (1) is open, is passed through
Adding pipe (21) injection liquid to reagent can flow into the reaction cup of accommodating chamber；
Photon counter (3), is arranged at measuring chamber (1) photon via, luminous produced for gathering reagent in reaction cup
Photon；
Shutter structure, is arranged between photon counter (3) and measuring chamber (1), can close or open photon via, including
Rotary drive (41) on installing plate (40), the baffle plate (42) with through hole (421), baffle plate is also formed on (42)
Chute (411), the trundle (422) that part (41) driven for rotation drives and rotated is arranged in chute (411), trundle
(422) when rotating, by the cooperation with chute (411), move horizontally baffle plate (42), so that the through hole on baffle plate (42)
(421) overlap or misplace with photon via；
Order performs following steps：
S1：Starting switch lid actuator (14) makes top cover (2) open measuring chamber (1) opening, and reaction cup is sent into measuring chamber (1)
In accommodating chamber；
S2：Starting switch lid actuator (14) makes top cover (2) closed measurement chamber (1) be open, and starts rotary drive (41) and make
Through hole (421) on baffle plate (42) is overlapped with photon via, starts photon counter (3)；
S3：Add pipe (21) by reagent and added into reaction cup and start agent and exciting agent；
S4：Make through hole (421) and photon via on baffle plate (42) wrong through after a period of time, starting rotary drive (41)
Position；
S5：Starting switch lid actuator (14) makes top cover (2) open measuring chamber (1) opening, takes reaction cup away.
2. the luminous measuring method of single reaction cup according to claim 1, it is characterised in that the time is 5 seconds in S4 steps.
3. the luminous measuring method of single reaction cup according to claim 1, it is characterised in that photon counter (3) is photoelectricity
Multiplier tube.
4. the luminous measuring method of single reaction cup according to claim 3, it is characterised in that the continuous acquisition light in S4 steps
Son obtains the total light yield in a period of time.
5. the luminous measuring method of single reaction cup according to claim 1, it is characterised in that in S1 steps, starting switch lid
Actuator (14) makes top cover (2) open before measuring chamber (1) opening, first determines through hole (421) and photon via on baffle plate (42)
In coincidence status, if the through hole (421) on baffle plate (42) is in misaligned state with photon via, the drive of starting switch lid is unable to
Moving part (14).
6. the luminous measuring method of single reaction cup according to claim any one of 1-5, it is characterised in that reaction cup area is most
Big one side alignment photon counter (3).
7. the luminous measuring method of single reaction cup according to claim 6, it is characterised in that reaction cup central point and photon meter
The central point alignment of the daylighting camera lens of number device (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710507536.6A CN107271433B (en) | 2017-06-28 | 2017-06-28 | A kind of luminous measurement method of single reaction cup |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710507536.6A CN107271433B (en) | 2017-06-28 | 2017-06-28 | A kind of luminous measurement method of single reaction cup |
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| CN107271433A true CN107271433A (en) | 2017-10-20 |
| CN107271433B CN107271433B (en) | 2019-07-30 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109374258A (en) * | 2018-09-26 | 2019-02-22 | 苏州长光华医生物医学工程有限公司 | A kind of photomultiplier tube test structure and luminous value measurement method |
| CN110412258A (en) * | 2018-04-28 | 2019-11-05 | 上海索昕生物科技有限公司 | Full-automatic light-induced chemiluminescent detector |
| CN110412019A (en) * | 2018-04-28 | 2019-11-05 | 上海索昕生物科技有限公司 | A kind of detection device and full-automatic light-induced chemiluminescent detector |
| CN112630215A (en) * | 2020-11-30 | 2021-04-09 | 苏州长光华医生物医学工程有限公司 | Multi-reaction-cup continuous measurement luminescence measurement device and luminescence measurement method |
| WO2022174511A1 (en) * | 2021-02-20 | 2022-08-25 | 重庆博奥新景医学科技有限公司 | Adaptive measurement and calculation method applied to luminescence value of chemiluminescence analyzer |
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| CN110412258A (en) * | 2018-04-28 | 2019-11-05 | 上海索昕生物科技有限公司 | Full-automatic light-induced chemiluminescent detector |
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| WO2022174511A1 (en) * | 2021-02-20 | 2022-08-25 | 重庆博奥新景医学科技有限公司 | Adaptive measurement and calculation method applied to luminescence value of chemiluminescence analyzer |
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| Publication number | Publication date |
|---|---|
| CN107271433B (en) | 2019-07-30 |
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