CN107253885A - A kind of high temperature Pleuotus nebrodensis culture matrix - Google Patents
A kind of high temperature Pleuotus nebrodensis culture matrix Download PDFInfo
- Publication number
- CN107253885A CN107253885A CN201710624671.9A CN201710624671A CN107253885A CN 107253885 A CN107253885 A CN 107253885A CN 201710624671 A CN201710624671 A CN 201710624671A CN 107253885 A CN107253885 A CN 107253885A
- Authority
- CN
- China
- Prior art keywords
- high temperature
- culture matrix
- parts
- temperature
- pleuotus nebrodensis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention belongs to Pleurotus nebrodensis culture matrix technical field, and in particular to a kind of high temperature Pleuotus nebrodensis culture matrix, including following raw material：55 60 parts of structure wood chip, 8 15 parts of wild marjoram oil-bound distemper, 8 15 parts of wheat bran skin, 8 15 parts of husk, 1 part of white sugar, 1 part of calcium carbonate, 1 part of pulverized limestone.The present invention has advantages below compared with prior art：By using hot sulfuric acid vapor to wheat bran skin, husk processing, wherein hemicellulose and cellulose degradation can be promoted, make the increase of its content of reducing sugar, cooled down again in cooling chamber, make its stable components, then with wild marjoram oil-bound distemper ball mill ball milling, reconfigure molecular structure, it is set to contain phenols hormonal substance, simultaneously rich in a variety of nutriments, the fast-growth of Pleurotus nebrodensis can be conducive to as matrix, mycelia can cover with bacterium bag within 37 days or so, regularity is good, the pure white stalwartness of mycelia, single bag of yield is up to 200g or so, the close hypertrophy of the cultivated pure white matter of mushroom body, commodity is good, Vc content is high.
Description
Technical field
The invention belongs to Pleurotus nebrodensis culture matrix technical field, and in particular to a kind of high temperature Pleuotus nebrodensis culture matrix.
Background technology
Bailing mushroom shape is attractive in appearance, and mouthfeel is delicate, rich in protein, fat, carbohydrate and several amino acids and Wei Sheng
Element, Mineral Elements Content extremely enriches, with very high nutritive value, while also having higher medical value, with anti-inflammatory
Analgesic, antibechic, desinsection and preventing and treating gynecological disease and antineoplastic effect, its fungi polysaccharide contained can effectively strengthen human immunity
Power, regulation Human Physiology balance, the Pleurotus nebrodensis cultivated at this stage is generally low form Pleurotus nebrodensis, because mycelia is dilute after mycelia purseful
Dredge, lack of staying power, it is impossible to direct fruiting, accordingly, it would be desirable to latter stage of ripening, cause fruiting irregular or not fruiting, and high temperature Pleuotus nebrodensis
It is the kind for taming out in low temperature Pleurotus nebrodensis extrusion, it is not necessary to directly can be with fruiting after bacteria, mycelia purseful, fruiting is neat
Yield is high, and cultivation period is greatly shortened, and its mouthfeel and nutritive value are unaffected, therefore widely promote；
High temperature Pleuotus nebrodensis is the new varieties tamed and come on the basis of low form kind, remains low form nutrition wide in variety
While value, the cultivation technique difficulty of original variety is reduced, with the constantly improve to Pleurotus nebrodensis silk cultural method, is carried
Go out and prepared mixed-matrix by adding 20-40% cotton seed hulls in structure wood chip matrix, be conducive to Pleurotus nebrodensis mycelial growth, because pure
Paper mulberry wood chip can suppress Pleurotus nebrodensis mycelial growth, be unfavorable for the cultivation of Pleurotus nebrodensis, can have by adding a certain amount of cotton seed hulls
Effect promotes Pleurotus nebrodensis mycelial growth, improves the exterior quality of yield and mushroom body, but its yield is relatively low compared to pure Cottonseed Hull, and
The profit of Pleurotus nebrodensis cultural hypha is more and more lower in the prior art, therefore its production how is improved in the case where ensureing mushroom body quality
Measure into the present technical issues that need to address.
The content of the invention
The purpose of the present invention is that there is provided one kind the problem of can not ensureing its quality for existing high temperature Pleuotus nebrodensis matrix
High temperature Pleuotus nebrodensis culture matrix.
The present invention is achieved by the following technical solutions：A kind of high temperature Pleuotus nebrodensis culture matrix, by weight, including
Following raw material：55-60 parts of structure wood chip, 8-15 parts of wild marjoram oil-bound distemper, 8-15 parts of wheat bran skin, 8-15 parts of husk, 1 part of white sugar, calcium carbonate 1
Part, 1 part of pulverized limestone；
Its preparation method is to be put into after being well mixed wheat bran skin, husk in closed container, quality is passed through under nitrogen atmosphere dense
The sulfuric acid vapours that degree is 12-15%, temperature is 75-80 DEG C, after being kept for 5-8 minutes, is cooled to normal temperature, mixed material is sent into
In cooling chamber, placed 4-6 hours in temperature is 2-5 DEG C of condition；Then by the material after processing and wild marjoram oil-bound distemper in ball milling
Sealed in tank, vacuumize in ball grinder and be filled with nitrogen, quality of the ball mill ratio is to be mixed with other raw materials after the completion of 20-25, ball milling
.
As further improvement of these options, the rotational speed of ball-mill is 350-400 revs/min, and Ball-milling Time is 35-
45 minutes.
As further improvement of these options, the strain properties of the master clock of the high temperature Pleuotus nebrodensis are, mycelia it is suitable
Suitable growth temperature is 22-26 DEG C, and suitable fruiting temperature is 18-25 DEG C.
As further improvement of these options, the matrix loads 17 × 33 × 0.05cm polypropylene fruiting bag,
Sterilized 1 hour under conditions of high pressure 0.24MPa before inoculation.
As further improvement of these options, the Pleurotus nebrodensis incubation includes inoculation, lucifuge culture, at mycelium stimulation
Reason, flower bud and the several steps of harvesting, wherein, flower bud stage day and night temperature is not less than 10 DEG C, and former base keeps 350- after occurring
450Lx and 650-850Lx crossover is for irradiation；The crossover of the 350-450Lx and 650-850Lx are all for the alternating of irradiation
Phase is 3 hours, and wherein the light irradiation time of 350-450Lx intensity is 2.5 hours, and the light of 650-850Lx intensity irradiates
Time is 0.5 hour, daily the 3-4 cycle of irradiation, and fruiting speed is can speed up compared with prior art, improves biology conversion
Rate.
Wherein, the harvesting includes harvesting twice, carries out second of mycelium stimulation processing after the completion of harvesting for the first time, and use barley
Root leachate is to bacterium bag moisturizing；The mass concentration of the Barley Roots leachate is 0.1-0.2%, be can help to ensure that second
Pleurotus nebrodensis Fruiting quality.
The present invention has advantages below compared with prior art：, can by using hot sulfuric acid vapor to wheat bran skin, husk processing
Promote wherein hemicellulose and cellulose degradation, make the increase of its content of reducing sugar, then cooled down in cooling chamber, make its composition steady
It is fixed, then with wild marjoram oil-bound distemper ball mill ball milling, molecular structure is reconfigured, it is contained phenols hormonal substance, simultaneously
Rich in a variety of nutriments, the fast-growth of Pleurotus nebrodensis can be conducive to as matrix, mycelia can cover with bacterium bag within 37 days or so,
Regularity is good, the pure white stalwartness of mycelia, and single bag of yield cultivates the close hypertrophy of the pure white matter of mushroom body up to 200g or so, and commodity is good, dimension
C content is high.
Embodiment
Embodiment 1
A kind of high temperature Pleuotus nebrodensis culture matrix, by weight, including following raw material：58 parts of structure wood chip, 12 parts of wild marjoram oil-bound distemper, wheat
12 parts of wheat bran, 12 parts of husk, 1 part of white sugar, 1 part of calcium carbonate, 1 part of pulverized limestone；
Its preparation method is to be put into after being well mixed wheat bran skin, husk in closed container, quality is passed through under nitrogen atmosphere dense
The sulfuric acid vapours that degree is 12-15%, temperature is 75-80 DEG C, after being kept for 5-8 minutes, is cooled to normal temperature, mixed material is sent into
In cooling chamber, placed 4-6 hours in temperature is 2-5 DEG C of condition；
Then the material after processing and wild marjoram oil-bound distemper are sealed in ball grinder, is vacuumized in ball grinder and be filled with nitrogen, ball milling
Mass ratio is 20-25, and the rotational speed of ball-mill is 350-400 revs/min, and Ball-milling Time is 35-45 minutes, with it after the completion of ball milling
He mixes raw material.
Wherein, the strain properties of the master clock of the high temperature Pleuotus nebrodensis are that the suitable growth temperature of mycelia is 22-26 DEG C, is fitted
Suitable fruiting temperature is 18-25 DEG C；
The matrix loads 17 × 33 × 0.05cm polypropylene fruiting bag, is gone out before inoculation under conditions of high pressure 0.24MPa
Bacterium 1 hour；
The Pleurotus nebrodensis incubation includes inoculation, lucifuge culture, mycelium stimulation processing, flower bud and harvests several steps, wherein, urge
Flower bud stage day and night temperature is not less than 10 DEG C, and former base keeps 350-450Lx and 650-850Lx crossover for irradiation after occurring；Institute
It is 3 hours, the wherein light of 350-450Lx intensity that 350-450Lx and 650-850Lx crossover, which is stated, for the alternate cycle of irradiation
Line irradiation time is 2.5 hours, and the light irradiation time of 650-850Lx intensity is 0.5 hour, daily the 3-4 cycle of irradiation.
Wherein, the harvesting includes harvesting twice, carries out second of mycelium stimulation processing after the completion of harvesting for the first time, and use barley
Root leachate is to bacterium bag moisturizing；The mass concentration of the Barley Roots leachate is 0.1-0.2%.
Embodiment 2
A kind of high temperature Pleuotus nebrodensis culture matrix, by weight, including following raw material：60 parts of structure wood chip, 8 parts of wild marjoram oil-bound distemper, wheat
15 parts of wheat bran, 8 parts of husk, 1 part of white sugar, 1 part of calcium carbonate, 1 part of pulverized limestone；Remaining content is in the same manner as in Example 1.
Embodiment 3
A kind of high temperature Pleuotus nebrodensis culture matrix, by weight, including following raw material：55 parts of structure wood chip, 15 parts of wild marjoram oil-bound distemper, wheat
8 parts of wheat bran, 15 parts of husk, 1 part of white sugar, 1 part of calcium carbonate, 1 part of pulverized limestone；Remaining content is in the same manner as in Example 1.
Control group 1 is set, wild marjoram oil-bound distemper and associative operation in embodiment 1 removed, remaining content and phase in embodiment 1
Together；Control group 2 is set, husk in embodiment 1 is replaced with etc. to the wheat bran of weight, remaining content is constant；Control group 3 is set, will
Each raw material is directly puddled uniformly in embodiment 1, and remaining content is constant；Control group 4 is set, former base in embodiment 1 is occurred
Keep 350-450Lx and 650-850Lx crossover for irradiation afterwards, 350-500Lx light prolonged exposure is replaced with, in remaining
Hold constant；Control group 5 is set, Barley Roots leachate in embodiment 1 light water is replaced with into, remaining content is constant；Control is set
Group 6, pleurotus nebrodensis culture medium formula is as follows by weight ratio：76 parts of paper mulberry wood chip, 20 parts of wheat bran, 1 part of white sugar, 1 part of calcium carbonate,
1 part of pulverized limestone, and conventionally cultivate；
In experiment, 40 fruiting bags are made per group of formula, matrix material-water ratio is 1：:15, pH value is 6-8, detects the purseful time,
Average per unit area yield, biological efficiency, Vc content and mushroom shape, the detection method of wherein Vc content is, by white spirit to be detected
After mushroom vacuum freeze drying, ascorbic content is detected using 2,6- dichloroindophenols titration, to its Vc after harvesting twice
Content is detected, is respectively labeled as A groups and B groups, obtains following result：
Table 1
| Group | The purseful time（My god） | Average per unit area yield（G/ bags） | Biological efficiency（%） | A groups（mg/100g） | A groups（mg/100g） | Mushroom body character |
| Embodiment 1 | 36.84 | 216.8 | 47.2 | 731.6 | 728.5 | Pure white, Gai great, matter are close |
| Embodiment 2 | 37.23 | 215.4 | 47.4 | 729.8 | 726.3 | Pure white, Gai great, matter are close |
| Embodiment 3 | 36.98 | 216.7 | 47.3 | 730.5 | 727.8 | Pure white, Gai great, matter are close |
| Control group 1 | 37.48 | 204.8 | 46.8 | 718.4 | 713.5 | Pure white, Gai great, matter are close |
| Control group 2 | 38.25 | 209.3 | 46.9 | 720.5 | 715.8 | Pure white, Gai great, matter are close |
| Control group 3 | 37.63 | 202.5 | 46.5 | 716.8 | 709.4 | Pure white, Gai great, matter are close |
| Control group 4 | 42.67 | 213.7 | 46.2 | 730.5 | 724.5 | Pure white, Gai great, matter are close |
| Control group 5 | 38.59 | 214.8 | 46.7 | 728.6 | 672.8 | Yellowing, Gai great, matter are close |
| Control group 6 | 39.25 | 181.2 | 45.8 | 712.5 | 683.5 | Pure white, Gai great, matter are close |
The change that can be seen that 5 conditionals of control by data in table 1 can influence the quality of Pleurotus nebrodensis after second of fruiting, its
Remaining condition can influence Pleurotus nebrodensis yield or the speed of growth, consider, and culture matrix can obtain mushroom body quality in embodiment 1-3
Preferable Pleurotus nebrodensis, has prominent directive significance to the cultivation of high temperature Pleuotus nebrodensis in the prior art.
Claims (8)
1. a kind of high temperature Pleuotus nebrodensis culture matrix, it is characterised in that by weight, including following raw material：Structure wood chip 55-60
Part, 8-15 parts of wild marjoram oil-bound distemper, 8-15 parts of wheat bran skin, 8-15 parts of husk, 1 part of white sugar, 1 part of calcium carbonate, 1 part of pulverized limestone；
Its preparation method is to be put into after being well mixed wheat bran skin, husk in closed container, quality is passed through under nitrogen atmosphere dense
The sulfuric acid vapours that degree is 12-15%, temperature is 75-80 DEG C, after being kept for 5-8 minutes, is cooled to normal temperature, mixed material is sent into
In cooling chamber, placed 4-6 hours in temperature is 2-5 DEG C of condition；Then by the material after processing and wild marjoram oil-bound distemper in ball milling
Sealed in tank, vacuumize in ball grinder and be filled with nitrogen, quality of the ball mill ratio is to be mixed with other raw materials after the completion of 20-25, ball milling
.
2. a kind of high temperature Pleuotus nebrodensis culture matrix as claimed in claim 1, it is characterised in that the rotational speed of ball-mill is 350-400
Rev/min, Ball-milling Time is 35-45 minutes.
3. a kind of high temperature Pleuotus nebrodensis culture matrix as claimed in claim 1, it is characterised in that the master clock of the high temperature Pleuotus nebrodensis
Strain properties are that the suitable growth temperature of mycelia is 22-26 DEG C, and suitable fruiting temperature is 18-25 DEG C.
4. a kind of high temperature Pleuotus nebrodensis culture matrix as claimed in claim 1, it is characterised in that the matrix loading 17 × 33 ×
0.05cm polypropylene fruiting bag, sterilizes 1 hour before inoculation under conditions of high pressure 0.24MPa.
5. a kind of high temperature Pleuotus nebrodensis culture matrix as claimed in claim 1, it is characterised in that the Pleurotus nebrodensis incubation includes
Inoculation, lucifuge culture, mycelium stimulation processing, flower bud and the several steps of harvesting, wherein, flower bud stage day and night temperature is not less than 10 DEG C,
Former base keeps 350-450Lx and 650-850Lx crossover for irradiation after occurring.
6. a kind of high temperature Pleuotus nebrodensis culture matrix as claimed in claim 5, it is characterised in that the 350-450Lx and 650-
850Lx crossover is 3 hours for the alternate cycle of irradiation, and wherein the light irradiation time of 350-450Lx intensity is 2.5 small
When, the light irradiation time of 650-850Lx intensity is 0.5 hour, daily the 3-4 cycle of irradiation.
7. a kind of high temperature Pleuotus nebrodensis culture matrix as claimed in claim 5, it is characterised in that the harvesting includes harvesting twice,
Second of mycelium stimulation processing is carried out after the completion of harvesting for the first time, and with Barley Roots leachate to bacterium bag moisturizing.
8. a kind of high temperature Pleuotus nebrodensis culture matrix as claimed in claim 7, it is characterised in that the quality of the Barley Roots leachate
Concentration is 0.1-0.2%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710624671.9A CN107253885A (en) | 2017-07-27 | 2017-07-27 | A kind of high temperature Pleuotus nebrodensis culture matrix |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710624671.9A CN107253885A (en) | 2017-07-27 | 2017-07-27 | A kind of high temperature Pleuotus nebrodensis culture matrix |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107253885A true CN107253885A (en) | 2017-10-17 |
Family
ID=60025875
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710624671.9A Pending CN107253885A (en) | 2017-07-27 | 2017-07-27 | A kind of high temperature Pleuotus nebrodensis culture matrix |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107253885A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220522A (en) * | 2018-09-25 | 2019-01-18 | 江苏农林职业技术学院 | Pleurotus nebrodensis cultivating process, culture medium based on dendrobium candidum and needle mushroom raw material |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1500377A (en) * | 2002-11-18 | 2004-06-02 | 兵 薛 | Industrialized production method of bai-ling mushroom |
| CN101627699A (en) * | 2009-08-04 | 2010-01-20 | 郑州大学 | Method for planting edible fungi and producing artificial grass peat by using cabo |
| CN102584398A (en) * | 2012-02-21 | 2012-07-18 | 李传华 | Culture medium, fruiting bag and high-yield culture technique of Pleurotus nebrodensis |
| CN103467174A (en) * | 2013-08-15 | 2013-12-25 | 安徽中祝农业发展有限公司 | Pleurotus nebrodensis cultivation medium and preparation method thereof |
| CN104823703A (en) * | 2015-04-21 | 2015-08-12 | 吴中区胥口精益生物医药研究所 | Culture method for pleurotus nebrodensis |
| CN105399471A (en) * | 2015-11-30 | 2016-03-16 | 陶超杰 | Pleurotus nebrodensis nutrition bag and preparation method thereof |
-
2017
- 2017-07-27 CN CN201710624671.9A patent/CN107253885A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1500377A (en) * | 2002-11-18 | 2004-06-02 | 兵 薛 | Industrialized production method of bai-ling mushroom |
| CN101627699A (en) * | 2009-08-04 | 2010-01-20 | 郑州大学 | Method for planting edible fungi and producing artificial grass peat by using cabo |
| CN102584398A (en) * | 2012-02-21 | 2012-07-18 | 李传华 | Culture medium, fruiting bag and high-yield culture technique of Pleurotus nebrodensis |
| CN103467174A (en) * | 2013-08-15 | 2013-12-25 | 安徽中祝农业发展有限公司 | Pleurotus nebrodensis cultivation medium and preparation method thereof |
| CN104823703A (en) * | 2015-04-21 | 2015-08-12 | 吴中区胥口精益生物医药研究所 | Culture method for pleurotus nebrodensis |
| CN105399471A (en) * | 2015-11-30 | 2016-03-16 | 陶超杰 | Pleurotus nebrodensis nutrition bag and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220522A (en) * | 2018-09-25 | 2019-01-18 | 江苏农林职业技术学院 | Pleurotus nebrodensis cultivating process, culture medium based on dendrobium candidum and needle mushroom raw material |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103333022B (en) | Cultivation material compatibility of agrocybe cylindracea and manufacture method of cultivation material | |
| CN105248150B (en) | A kind of method of cordycepin content in raising fruiting bodies of cordyceps militaris | |
| CN105532257A (en) | Pleurotusostreatus and pleurotus eryngii high-yield cultivation method | |
| CN104969773B (en) | The method for obtaining Cordyceps militaris fermentate or fructification using sweet potato dregs fermentation | |
| CN106613355A (en) | Ecological planting method of oyster mushrooms | |
| CN107853077A (en) | A kind of solid Se-rich lucid ganoderma culture medium and its method for producing ganoderma lucidum | |
| CN105110961A (en) | Liquid fermentation culture medium for shiitake mushrooms and method for producing shiitake mushrooms through the same | |
| CN107360858A (en) | A kind of breeding method of mushroom edible mushroom | |
| CN106348919A (en) | Hypsizygus marmoreus culture medium and method for culturing Hypsizygus marmoreus by using culture medium | |
| CN104303830A (en) | Planting method of Pleurotus eryngii | |
| CN105543104A (en) | Optimization method of solid culture medium for artificial acclimation and cultivation of wild Isaria cicadae Miquel | |
| CN103583236A (en) | Method for preparing fleckedflesh polypore mycelium | |
| CN103570440A (en) | Edible mushroom culture medium adopting banana skin as raw material | |
| KR100823541B1 (en) | Mushroom cultivation method | |
| CN105237248A (en) | Grifola frondosa production culture medium and application thereof | |
| CN107396753A (en) | A kind of cultivation technique of Silicon-rich flat mushroom | |
| CN107473791A (en) | Planting almond abalone mushroom matrix | |
| CN109997608A (en) | A kind of implantation methods of edible mushroom | |
| CN102948325A (en) | Cordyceps militaris efficient quick cultivation technology | |
| CN101717309A (en) | Culture medium for straw rotting edible fungi solid strain and method for preparing solid strain | |
| CN104885787A (en) | Planting method for cordyceps militaris sporocarp high in active matter content | |
| CN102318544A (en) | A kind of Cordyceps militaris ciltivating process | |
| CN104541967A (en) | Production method for extracting mushroom mycelium of lentinan | |
| CN102229509B (en) | Liquid culture medium applicable to various edible fungi | |
| CN101337839A (en) | Edible fungus culture medium of citrus skin slag and method for preparing same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171017 |