CN107242351A - 一种益生菌组合物及其应用 - Google Patents
一种益生菌组合物及其应用 Download PDFInfo
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Abstract
本发明提供了一种益生菌组合物，每100g含：膳食纤维35～80％；菊粉15～60％；低聚木糖5～15％；嗜酸乳杆菌10～50亿CFU；鼠李糖乳杆菌TR08 10～30亿CFU；植物乳杆菌20～40亿CFU；副干酪乳杆菌10～30亿CFU；长双歧杆菌10～20亿CFU；乳双歧杆菌10～30亿CFU。本发明提供的组合物制备工艺简单、成本低廉、使用方便，可提高宠物食欲、改善宠物肠道微生态环境、防治宠物腹泻、提高宠物免疫能力。
Description
技术领域
本发明属于生物技术领域，特别涉及一种益生菌组合物，还涉及该组合物的应用。
背景技术
随着人民生活水平的提高，饲养宠物的人越来越多，宠物需要的饲料要求也越来越 精细化。然而，一方面，现在宠物饲料原材料基本相同，主要成分类似，长期食用，会 引起食欲不振，胃胀，宠物目光无神，毛发暗晦，粗糙，精神不好，经常睡觉，导致生 病；另一方面，家养的宠物长期食用的狗粮、猫粮、零食等为加工食品，各种食品添加 剂的摄入会持续破坏宠物肠胃道菌群平衡，容易产生便秘、拉稀、消化不良、肠胃炎等 肠胃疾病。生病一般上宠物医院，但是长期服用药物会造成依赖性，同时药物是化学合 成的，存在着或多或少的副作用，而且宠物一般比较抗拒吃药，打针。此外，目前也未 有可供宠物长期食用的饲料添加剂以解决现有技术的问题。
发明内容
技术问题：为了解决现有技术的缺陷，本发明提供了一种宠物用益生菌组合物。
技术方案：本发明提供的一种益生菌组合物，每100g含：
作为优选，所述益生菌组合物，每100g含：
作为另一种优选，所述益生菌组合物，每100g含：
本发明还提供了上述益生菌组合物在制备宠物饲料添加剂中的应用。
本发明还提供了上述益生菌组合物在制备调节宠物肠道菌群平衡的饲料添加剂中的 应用。
本发明还提供了上述益生菌组合物在制备防治宠物腹泻的饲料添加剂中的应用。
本发明还提供了上述益生菌组合物在制备提高宠物食欲的饲料添加剂中的应用。
本发明还提供了上述益生菌组合物在制备提高宠物免疫能力的饲料添加剂中的应 用。
本发明还提供了上述益生菌组合物在制备提高宠物免疫能力的饲料添加剂中的应 用。
有益效果：本发明提供的组合物制备工艺简单、成本低廉、使用方便，可提高宠物食欲、改善宠物肠道微生态环境、防治宠物腹泻、提高宠物免疫能力。
具体而言，本发明益生菌组合物相对于现有技术具有以下突出的优势：
该益生菌组合物通过以下原理改善宠物肠道微生态环境：
(1)补充：该组合物首先通过快速补充更有效的益生菌活力组合，包括源于肠道内的乳酸菌、双歧杆菌以及为在肠道内繁殖创造环境的凝结芽孢杆菌，该益生菌组合是高 活性、高定植率的肠道益生菌活力组合，可快速补充至肠道内，使肠内益生菌数量恢复 至健康水平。
(2)抑制：该组合物中的益生菌可有效抑制损害肠道功能、诱发各种疾病的有害菌；特定的益生菌活力组合通过夺取有害菌生存需要的氧，产生可抑制有害菌生长的乳酸，占领有害菌在肠道的粘附位置，多方位有效抑制有害菌的生长，恢复健康的肠道微 生态环境。
(3)重建：该组合物可修复重建肠道生物屏障，增强免疫，促进营养吸收。该组 合物可定植在肠道粘膜表面，修复并重建被破坏的肠道生物屏障，阻止致病菌及毒素的 入侵，诱导肠粘膜产生各种抗体，增强免疫，真正使肠道长治久安。
(4)协同：该组合物具备优秀的协同能力，可与多种药品、营养品、食品长期搭 配服用，促进机体对营养的吸收，减轻多种药物的毒副作用，让肠道倍添活力。
该益生菌组合物用途应用广泛：
(1)该组合物可用于调节肠道菌群，保护肠胃，抑制有害菌生长，对宠物腹泻、便秘、急慢性肠炎有良好疗效；
(2)该组合物可用于宠物手术及抗生素治疗后的恢复期，可迅速改善肠道微生态环境，提高健康水平；
(3)该组合物可促进消化与吸收，消除宠物因肠胃紊乱导致的口臭降低排泄臭气，使排泄物成型，改善喂养环境；
(4)该组合物可显著改善宠物挑食偏食，促进体内多种消化酶维生素及营养物质的产生，增强免疫力；
(5)该组合物可减少犬猫因断奶换料、免疫驱虫、环境变化引起的腹泻等各种应激反应。
附图说明
图1为根据毒株特异性PCR检测到嗜酸乳杆菌对粪便中的嗜酸乳杆菌进行计数结果 图。
具体实施方式
下面对本发明益生菌组合物作出进一步说明。
鼠李糖乳杆菌为鼠李糖乳杆菌(L.rhamnosus)TR08，其分类命名为鼠李糖氏乳杆菌 (Lactobacillus rhamnosus)TR08，在中国典型培养物保藏中心(简称CCTCC)保藏， 保藏单位地址：湖北省武汉市武昌区武汉大学，邮政编码为430072，其保藏编号为： CCTCCNO.M 2016548，保藏日期为2016年10月10日。
实施例1
益生菌组合物，每100g含：
实施例2
益生菌组合物，每100g含：
实施例3
益生菌组合物，每100g含：
实施例4
益生菌组合物，每100g含：
实施例5
益生菌组合物，每100g含：
实施例6
益生菌组合物，每100g含：
本发明组合物的药效学评价。
实验药物：实施例1至6的组合物；
实验动物：20日龄幼犬210只，分为7组(实验组1至实验组6、对照组)，分别给 予实验药物和对照药物。
喂养方法：
(1)实验组：20～30日龄饲喂混有本发明组合物的奶粉；30日龄断乳后饲喂混有复合乳酸菌的饲料，以2次/日饲喂；
(2)对照组：20～30日龄饲喂奶粉；30日龄断乳后饲喂饲料，以2次/日饲喂。
另选择5只幼犬，根据毒株特异性PCR检测到嗜酸乳杆菌对粪便中的嗜酸乳杆菌进行计数，第1天到第3天，给狗喂食(1.7亿CFU/天)含嗜酸乳杆菌的酸奶，分别收集 粪便样本，进行检测，结果，见图1，表明：嗜酸乳杆菌可以有效的在狗肠道的生存。
检测实验组和对照组的幼犬血液生化指标，见表1。
表1实验组和对照组的幼犬血液生化指标
检测实验组和对照组的幼犬血浆MDA含量、SOD和GSH-PX活性，见表2。
表2实验组和对照组的幼犬血浆MDA含量、SOD和GSH-PX活性
| 项目 | MDA/umol.ml-1 | SOD/U.ml-1 | GSH-PX/U.ml-1 |
| 实验组1 | 5.66±0.83 | 19.80±9.00 | 2266.33±281.53 |
| 实验组2 | 5.71±0.61 | 19.18±12.60 | 2200.38±187.77 |
| 实验组3 | 5.65±0.81 | 19.68±10.46 | 2246.28±195.82 |
| 实验组4 | 5.70±0.76 | 19.57±11.51 | 2249.64±218.6 |
| 实验组5 | 5.67±0.79 | 19.91±10.89 | 2219.78±268.4 |
| 实验组6 | 5.28±0.56 | 20.76±10.82 | 2408.92±192.58 |
| 对照组 | 6.98±0.81 | 16.05±6.64 | 1904.95±128.28 |
以上结果表明：
(1)采用本发明组合物喂食幼犬后，幼犬的血液总蛋白、球蛋白含量增加，而白球比降低，表明：本发明组合物可提高幼犬机体的免疫能力。
(2)采用本发明组合物喂食幼犬后，血浆尿素氮含量降低，表明：本发明组合物可提高幼犬对蛋白质的合成代谢，增加了氮在幼犬体内的沉积。
(3)本发明组合物能促进肠道运动，增强消化机能，提高血液中激素水平，促进机体的合成代谢，刺激动物免疫机能，通过免疫途径发挥营养作用。
(4)采用本发明组合物喂食幼犬后，幼犬血液SOD和GSH-PX活性提高，MDA 含量显著下降，表明：本发明组合物可提高幼犬的免疫能力和抗氧化能力。
统计幼犬腹泻时间和程度，见表3。
表3幼犬腹泻时间和程度
| 组别 | 重度 | 轻度 | 总计 |
| 实验组1 | 0 | 3 | 3 |
| 实验组2 | 0 | 2 | 2 |
| 实验组3 | 0 | 3 | 3 |
| 实验组4 | 0 | 3 | 3 |
| 实验组5 | 0 | 2 | 2 |
| 实验组6 | 0 | 0 | 0 |
| 对照组 | 3 | 12 | 15 |
其中，腹泻时间为1天以下(含)为轻度，腹泻时间为1天以上(不含)为重度。
统计各时期幼犬体重，见表4。
表4各时期幼犬体重
统计幼犬死亡率，见表5。
表5幼犬死亡率
| 组别 | 20 | 30 | 40 | 50 | 60 | 死亡数 | 死亡率/％ |
| 实验组1 | 0 | 1 | 1 | 0 | 0 | 2 | 6.67 |
| 实验组2 | 1 | 1 | 0 | 0 | 0 | 2 | 6.67 |
| 实验组3 | 1 | 0 | 1 | 0 | 0 | 2 | 6.67 |
| 实验组4 | 1 | 0 | 0 | 0 | 0 | 1 | 3.33 |
| 实验组5 | 0 | 1 | 1 | 0 | 0 | 2 | 6.67 |
| 实验组6 | 0 | 0 | 0 | 0 | 0 | 0 | 0.00 |
| 对照组 | 1 | 3 | 5 | 0 | 0 | 9 | 30.0 |
以上结果表明：采用本发明组合物喂食幼犬后，仔犬腹泻时间显著减少，体重增加较快，存活率较高。
同时，实验过程中发现：采用本发明组合物喂食幼犬后，可以提高宠物的胃口，减少呕吐反应。
SEQUENCE LISTING
<110> 南京天益鸿胜生物科技有限公司
<120> 一种益生菌组合物及其应用
<130> SC20161008002
<160> 2
<170> PatentIn version 3.3
<210> 1
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<212> DNA
<213> Lactobacillus reuteri
<220>
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<400> 1
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Claims (9)
1.一种益生菌组合物，其特征在于：每100g含：
2.根据权利要求1所述的一种益生菌组合物，其特征在于：每100g含：
3.根据权利要求1所述的一种益生菌组合物，其特征在于：每100g含：
4.权利要求1所述的益生菌组合物在制备宠物饲料添加剂中的应用。
5.权利要求1所述的益生菌组合物在制备调节宠物肠道菌群平衡的饲料添加剂中的应用。
6.权利要求1所述的益生菌组合物在制备防治宠物腹泻的饲料添加剂中的应用。
7.权利要求1所述的益生菌组合物在制备提高宠物食欲的饲料添加剂中的应用。
8.权利要求1所述的益生菌组合物在制备提高宠物免疫能力的饲料添加剂中的应用。
9.权利要求1所述的益生菌组合物在制备提高宠物免疫能力的饲料添加剂中的应用。
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107868764A (zh) * | 2017-11-27 | 2018-04-03 | 内蒙古普泽生物制品有限责任公司 | 一种增强宠物免疫力的复合益生菌粉制剂及其制备方法 |
| CN108077935A (zh) * | 2018-01-22 | 2018-05-29 | 广东格物生物科技有限公司 | 调理中老年人肠道功能菌剂及其制备方法和应用 |
| CN108902517A (zh) * | 2018-05-16 | 2018-11-30 | 浙江省中医药研究院 | 一种益生菌组合物及其应用 |
| WO2019102218A3 (en) * | 2017-11-23 | 2019-08-01 | Optibiotix Limited | Lactobacillus mixed with xylooligosaccharides, cellobiose and/or gentiooligosaccharides |
| CN112568332A (zh) * | 2020-12-02 | 2021-03-30 | 福建洛东生物技术有限公司 | 一种宠物饲料及益生菌宠物饲料添加剂 |
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| CN108323641A (zh) * | 2018-02-07 | 2018-07-27 | 上海宠幸宠物用品有限公司 | 一种用于调理宠物犬猫肠道功能的保健食品及其制备方法 |
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| ES2565407T3 (es) * | 2010-11-05 | 2016-04-04 | Nestec S.A. | Procedimiento para la preparación de un producto alimenticio para animales de compañía, el cual contiene microorganismos probióticos |
| CN106071261A (zh) * | 2016-03-22 | 2016-11-09 | 上海申亚动物保健品阜阳有限公司 | 一种促进贵宾犬消化吸收的益生菌添加剂及其制备方法 |
| CN106520598A (zh) * | 2016-10-25 | 2017-03-22 | 天益健康科学研究院（镇江）有限公司 | 一种鼠李糖乳杆菌及应用 |
| CN106509455A (zh) * | 2016-11-10 | 2017-03-22 | 四川理工学院 | 一种兔用复合型合生元微生态制剂及其制备方法 |
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| WO2019102218A3 (en) * | 2017-11-23 | 2019-08-01 | Optibiotix Limited | Lactobacillus mixed with xylooligosaccharides, cellobiose and/or gentiooligosaccharides |
| US11622980B2 (en) | 2017-11-23 | 2023-04-11 | Optibiotix Limited | Compositions comprising prebiotics and probiotics |
| CN107868764A (zh) * | 2017-11-27 | 2018-04-03 | 内蒙古普泽生物制品有限责任公司 | 一种增强宠物免疫力的复合益生菌粉制剂及其制备方法 |
| CN108077935A (zh) * | 2018-01-22 | 2018-05-29 | 广东格物生物科技有限公司 | 调理中老年人肠道功能菌剂及其制备方法和应用 |
| CN108902517A (zh) * | 2018-05-16 | 2018-11-30 | 浙江省中医药研究院 | 一种益生菌组合物及其应用 |
| CN112568332A (zh) * | 2020-12-02 | 2021-03-30 | 福建洛东生物技术有限公司 | 一种宠物饲料及益生菌宠物饲料添加剂 |
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