CN107238645A - 在线监测用葡萄糖氧化酶丝网印刷电极及其制备方法 - Google Patents
在线监测用葡萄糖氧化酶丝网印刷电极及其制备方法 Download PDFInfo
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- CN107238645A CN107238645A CN201710329483.3A CN201710329483A CN107238645A CN 107238645 A CN107238645 A CN 107238645A CN 201710329483 A CN201710329483 A CN 201710329483A CN 107238645 A CN107238645 A CN 107238645A
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- 238000007650 screen-printing Methods 0.000 title claims abstract description 26
- 235000019420 glucose oxidase Nutrition 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 108010015776 Glucose oxidase Proteins 0.000 title claims abstract description 12
- 239000004366 Glucose oxidase Substances 0.000 title claims abstract description 12
- 229940116332 glucose oxidase Drugs 0.000 title claims abstract description 12
- 238000012544 monitoring process Methods 0.000 title claims abstract description 7
- 108090000790 Enzymes Proteins 0.000 claims abstract description 45
- 102000004190 Enzymes Human genes 0.000 claims abstract description 45
- 229940088598 enzyme Drugs 0.000 claims abstract description 45
- DCYOBGZUOMKFPA-UHFFFAOYSA-N iron(2+);iron(3+);octadecacyanide Chemical compound [Fe+2].[Fe+2].[Fe+2].[Fe+3].[Fe+3].[Fe+3].[Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] DCYOBGZUOMKFPA-UHFFFAOYSA-N 0.000 claims abstract description 35
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- 229960003351 prussian blue Drugs 0.000 claims abstract description 35
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 21
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 21
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- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 22
- 229920002873 Polyethylenimine Polymers 0.000 claims description 21
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 239000003960 organic solvent Substances 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 11
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- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 9
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 8
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 8
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
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- 229920000915 polyvinyl chloride Polymers 0.000 claims description 8
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- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 4
- 235000011054 acetic acid Nutrition 0.000 claims description 4
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 4
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- 238000007654 immersion Methods 0.000 claims description 4
- BHTJEPVNHUUIPV-UHFFFAOYSA-N pentanedial;hydrate Chemical compound O.O=CCCCC=O BHTJEPVNHUUIPV-UHFFFAOYSA-N 0.000 claims description 4
- 230000035484 reaction time Effects 0.000 claims description 4
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 4
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
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- 238000003756 stirring Methods 0.000 claims description 4
- 238000000108 ultra-filtration Methods 0.000 claims description 4
- 238000004458 analytical method Methods 0.000 abstract description 11
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- 239000004615 ingredient Substances 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 25
- 239000008103 glucose Substances 0.000 description 21
- 238000004519 manufacturing process Methods 0.000 description 11
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- 239000000047 product Substances 0.000 description 8
- 238000004445 quantitative analysis Methods 0.000 description 6
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- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 3
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- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
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- 239000012491 analyte Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
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- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011946 reduction process Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/904—Oxidoreductases (1.) acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Electrochemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
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CN201710329483.3A CN107238645B (zh) | 2017-05-11 | 2017-05-11 | 在线监测用葡萄糖氧化酶丝网印刷电极及其制备方法 |
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Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110146563A (zh) * | 2018-12-17 | 2019-08-20 | 浙江大学山东工业技术研究院 | 一种基于普鲁士蓝的无酶尿酸传感器 |
CN110146562A (zh) * | 2018-12-17 | 2019-08-20 | 浙江大学山东工业技术研究院 | 一种基于普鲁士蓝的无酶尿酸传感器及其制备方法 |
CN110643499A (zh) * | 2019-09-27 | 2020-01-03 | 南京工业大学 | 一种微生物发酵在线浓度分析仪的上下位机通讯方法 |
CN111855774A (zh) * | 2020-05-19 | 2020-10-30 | 大连理工大学 | 一种基于普鲁士蓝的智能可穿戴酶基生物传感器及其制备方法和应用 |
CN112415062A (zh) * | 2020-11-27 | 2021-02-26 | 山东省科学院生物研究所 | 一种快速检测乙醇的电极系统及使用电极系统对乙醇检测的方法 |
WO2021083429A1 (zh) * | 2019-10-31 | 2021-05-06 | 山东省科学院生物研究所 | 一种密码子优化的葡萄糖氧化酶基因及其应用 |
CN114002283A (zh) * | 2021-11-26 | 2022-02-01 | 南京伊桥科技有限公司 | 一种高选择性电化学硫化氢传感器及工作电极制备方法 |
CN114002292A (zh) * | 2021-11-02 | 2022-02-01 | 安序源生物科技(深圳)有限公司 | 一种唾液血糖校正装置及方法 |
CN114334223A (zh) * | 2021-11-22 | 2022-04-12 | 北京大学 | 一种导电浆料及应用 |
CN115372448A (zh) * | 2022-10-26 | 2022-11-22 | 可孚医疗科技股份有限公司 | 一种葡萄糖检测卡及其制备方法 |
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US4288552A (en) * | 1978-04-19 | 1981-09-08 | Novo Industri A/S | Immobilized intracellular enzymes |
CN101929977A (zh) * | 2010-08-31 | 2010-12-29 | 刘丹 | 酶生物电化学传感芯片及其制备和使用方法 |
CN103757042A (zh) * | 2014-01-15 | 2014-04-30 | 华南理工大学 | 基于自聚集短肽诱导的固定化腈水解酶及制备方法和应用 |
CN104833713A (zh) * | 2015-04-23 | 2015-08-12 | 南京工业大学 | 一种简易的酶生物传感器的制备方法 |
CN104962546A (zh) * | 2015-06-19 | 2015-10-07 | 浙江工业大学 | 一种含葡萄糖异构酶细胞的固定化方法 |
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2017
- 2017-05-11 CN CN201710329483.3A patent/CN107238645B/zh active Active
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US4288552A (en) * | 1978-04-19 | 1981-09-08 | Novo Industri A/S | Immobilized intracellular enzymes |
CN101929977A (zh) * | 2010-08-31 | 2010-12-29 | 刘丹 | 酶生物电化学传感芯片及其制备和使用方法 |
CN103757042A (zh) * | 2014-01-15 | 2014-04-30 | 华南理工大学 | 基于自聚集短肽诱导的固定化腈水解酶及制备方法和应用 |
CN104833713A (zh) * | 2015-04-23 | 2015-08-12 | 南京工业大学 | 一种简易的酶生物传感器的制备方法 |
CN104962546A (zh) * | 2015-06-19 | 2015-10-07 | 浙江工业大学 | 一种含葡萄糖异构酶细胞的固定化方法 |
Non-Patent Citations (2)
Title |
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GANAPATI D. YADAV等: "Enzyme-catalysed optical resolution of mandelic acid via RS(∓)-methyl mandelate in non-aqueous media", 《BIOCHEMICAL ENGINEERING JOURNAL》 * |
綦翠华等: "果汁原浆中葡萄糖含量测定的酶电极设计", 《食品科学》 * |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110146562A (zh) * | 2018-12-17 | 2019-08-20 | 浙江大学山东工业技术研究院 | 一种基于普鲁士蓝的无酶尿酸传感器及其制备方法 |
CN110146563A (zh) * | 2018-12-17 | 2019-08-20 | 浙江大学山东工业技术研究院 | 一种基于普鲁士蓝的无酶尿酸传感器 |
CN110643499A (zh) * | 2019-09-27 | 2020-01-03 | 南京工业大学 | 一种微生物发酵在线浓度分析仪的上下位机通讯方法 |
WO2021083429A1 (zh) * | 2019-10-31 | 2021-05-06 | 山东省科学院生物研究所 | 一种密码子优化的葡萄糖氧化酶基因及其应用 |
CN111855774A (zh) * | 2020-05-19 | 2020-10-30 | 大连理工大学 | 一种基于普鲁士蓝的智能可穿戴酶基生物传感器及其制备方法和应用 |
CN112415062A (zh) * | 2020-11-27 | 2021-02-26 | 山东省科学院生物研究所 | 一种快速检测乙醇的电极系统及使用电极系统对乙醇检测的方法 |
CN114002292A (zh) * | 2021-11-02 | 2022-02-01 | 安序源生物科技(深圳)有限公司 | 一种唾液血糖校正装置及方法 |
CN114002292B (zh) * | 2021-11-02 | 2024-03-08 | 深圳清华大学研究院 | 一种唾液血糖校正装置及方法 |
CN114334223B (zh) * | 2021-11-22 | 2023-01-13 | 北京大学 | 一种导电浆料及应用 |
CN114334223A (zh) * | 2021-11-22 | 2022-04-12 | 北京大学 | 一种导电浆料及应用 |
CN114002283A (zh) * | 2021-11-26 | 2022-02-01 | 南京伊桥科技有限公司 | 一种高选择性电化学硫化氢传感器及工作电极制备方法 |
CN115372448B (zh) * | 2022-10-26 | 2023-02-14 | 可孚医疗科技股份有限公司 | 一种葡萄糖检测卡及其制备方法 |
CN115372448A (zh) * | 2022-10-26 | 2022-11-22 | 可孚医疗科技股份有限公司 | 一种葡萄糖检测卡及其制备方法 |
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