CN107213103B - Snail facial mask containing snail active enzyme mucus extract and its preparation method - Google Patents
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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Abstract
The invention relates to a snail facial mask containing snail active enzyme mucus extract and a preparation method thereof, belongs to the field of cosmetics, and particularly relates to a preparation method of a snail facial mask containing snail secretion filtrate. The invention uses water, glycerin, propylene glycol, snail secretion filtrate, hydrolyzed vegetable protein, ALOE vera (ALOE BARBADENSIS) extract, nacre extract, hyaluronic acid, dipotassium glycyrrhizinate, allantoin, disodium EDTA and propyl benzoate as raw materials. The snail facial mask provided by the invention is suitable for the skin with water deficiency, dryness and large pores.
Description
Technical Field
The invention mainly belongs to the field of cosmetics, and particularly relates to a snail facial mask containing a snail active enzyme mucus extract and a preparation method thereof.
Background
As early as in ancient Hizirca, the father of medicine, "Xibo Kela Zi", has used snails in medical therapy as a dietary therapy for whooping cough and gastric ulcer. The Bascunan family of Chilean (Bascunan family) began to breed snails in the year 1980 and exported in large quantities to Europe as a cooking holy. However, in handling the snails, they found that the skin of the worker became soft, and, when the worker was cut by the snail cage, it was very surprising to find that the wound healed quickly and did not infect, nor did the worker leave scars. At this time, the person who is one member of the bethan family-feinanduo bethan (ferando basicnan) is a student of medical department, and accurate scientific research for 15 years is carried out on chile 'Helix Aspersa Muller spiral snail', so that the extract can promote the repair of the skin and soften the skin; and applied for world patent, officially listed in Chile in 1995, and has raised a wind tide of 'natural regeneration' in various countries. Recently, snail mucus and snail enzyme have been widely used in cosmetics because of the natural collagen, elastin, allantoin, protein, multivitamins, glycolic acid, natural antimicrobial agents, etc.
However, most of the snail mucus and helicase used in the existing cosmetics are obtained by making the snail climb on a rough surface to secrete more mucus. There is a problem that the mucus obtained by this method contains a small amount of enzyme species and is poor in the effect of use. In addition, in the existing facial mask preparation process, only the snail mucus and the snail enzyme are simply mixed and stirred with other components, and the obtained facial mask liquid cannot be uniformly emulsified, or even if the intermolecular combination of the components of the obtained emulsion is poor, the mixing state is unstable, the components are easy to decompose and deteriorate, and the use effect is seriously influenced.
Disclosure of Invention
In order to solve the problems, the invention provides a snail facial mask of snail active enzyme mucus extract and a preparation method thereof, the snail facial mask contains up to 20 enzymes, and the components of the facial mask liquid are stable in molecular combination and are not easy to deteriorate.
The invention is realized by the following technical scheme:
a preparation method of snail facial mask containing snail secretion filtrate comprises mixing water, glycerol, propylene glycol, snail secretion filtrate, hydrolyzed vegetable protein, Aloe Barbadensis Miller extract, Concha Margaritifera extract, hyaluronic acid, dipotassium glycyrrhizinate, allantoin, EDTA disodium, and propyl benzoate; the method is characterized in that all the components are stirred at different temperature stages, so that all the components are fully and uniformly emulsified and all the molecules are stably combined.
Further, the method comprises the steps of:
heating and stirring: mixing water, glycerol, propylene glycol, snail secretion filtrate, hydrolyzed vegetable protein, Aloe Barbadensis Miller extract, Concha Margaritifera extract, hyaluronic acid, dipotassium glycyrrhizinate, allantoin, disodium EDTA, and propyl benzoate at a certain ratio, stirring under vacuum, controlling temperature to raise from room temperature to T1 while stirring, and raising temperature at 0.1-1 deg.C/min;
stirring at constant temperature: stirring for 2-5h at the temperature of T1;
degassing and stirring: degassing the mixed solution, and cooling to T2 temperature;
and (3) continuously stirring: stirring for 0.5-3h at the temperature of T2;
standing and aging: cooling to room temperature, and standing for 12-48 h;
wherein T1 is 60-80 ℃; t2 was 30-40 ℃.
Further, the preparation method of the snail secretion filtrate comprises the following steps:
stimulation of snail mucus secretion: the snail is placed in a box body to collect mucus, various pictures with sharp objects are arranged around the box body, or the box body is made of transparent materials, various objects with sharp parts are arranged around the outside of the box body, the snail is stimulated to secrete the mucus under the condition of not damaging the snail, and the mucus is collected;
extracting filtrate: collecting mucus, centrifuging at high speed to break mucus cell, and collecting supernatant to obtain snail secretion filtrate containing snail active enzyme.
Furthermore, during the preparation of the snail secretion filtrate, the mucus is required to be kept within 0-6 ℃, and during the centrifugation, the temperature is required to be kept below zero 3-6 ℃ to avoid the temperature rise during the centrifugation.
Further, the storage temperature of the collected mucus is within 0-5 ℃.
Further, the stimulating snail secretes mucus: the snail is placed in a box body to collect mucus, and the box body vibrates to stimulate the snail to secrete the mucus.
Further, the snail secretion filtrate obtained by stimulating the bursa and digestive tract of the snail contains cellulase, pectinase, amylase, and protease.
A snail facial mask containing snail secretion filtrate comprises water, glycerol, propylene glycol, snail secretion filtrate, hydrolyzed vegetable protein, Aloe Barbadensis Miller extract, Concha Margaritifera extract, hyaluronic acid, dipotassium glycyrrhizinate, allantoin, EDTA disodium, and propyl benzoate.
Further, the snail secretion filtrate obtained by stimulating the bursa and digestive tract of the snail contains cellulase, pectinase, amylase, and protease.
The invention has the beneficial technical effects that:
(1) the snail secretion filtrate of the present invention is obtained by stimulating the treble sacs and digestive tract of snails, and contains more enzymes and has a higher nutritional value than snail mucus obtained by snails crawling on rough surfaces.
(2) The preparation method of the invention controls the temperature, the stirring strength and the stirring time in the process of mixing and stirring the snail secretion filtrate and other components, so that the snail mucus and other components are fully emulsified, and the components are stably combined with each other.
Drawings
FIG. 1 is a schematic flow chart of a preparation method of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
On the contrary, the invention is intended to cover alternatives, modifications, equivalents and alternatives which may be included within the spirit and scope of the invention as defined by the appended claims. Furthermore, in the following detailed description of the present invention, certain specific details are set forth in order to provide a better understanding of the present invention. It will be apparent to one skilled in the art that the present invention may be practiced without these specific details.
Example 1
A method for preparing snail mask containing snail active enzyme mucus extract comprises the following steps:
heating and stirring: mixing water, glycerol, propylene glycol, snail secretion filtrate, hydrolyzed vegetable protein, Aloe Barbadensis Miller extract, Concha Margaritifera extract, hyaluronic acid, dipotassium glycyrrhizinate, allantoin, disodium EDTA, and propyl benzoate at a certain ratio, stirring under vacuum, controlling temperature to raise from room temperature to 60-80 deg.C during stirring, and raising temperature at 0.1-1 deg.C/min;
stirring at constant temperature: stirring for 2-5h at 60-80 deg.C;
degassing and stirring: degassing the mixed solution, and cooling to 30-40 deg.C;
and (3) continuously stirring: stirring at 30-40 deg.C for 0.5-3 h;
standing and aging: cooling to room temperature, and standing for 12-48 h.
Further, the preparation method of the snail secretion filtrate comprises the following steps:
stimulation of snail mucus secretion: the snail is placed in a box body to collect mucus, various pictures with sharp objects are arranged around the box body, or the box body is made of transparent materials, various objects with sharp parts are arranged around the outside of the box body, the snail is stimulated to secrete the mucus under the condition of not damaging the snail, and the mucus is collected;
extracting filtrate: collecting mucus, centrifuging at high speed to break mucus cell, and collecting supernatant to obtain snail secretion filtrate containing snail active enzyme.
Furthermore, during the preparation of the snail secretion filtrate, the temperature of mucus needs to be kept within 0-5 ℃, and during the centrifugation, the temperature needs to be kept below-3-6 ℃ to avoid the temperature rise during the centrifugation.
Further, the storage temperature of the collected mucus is within 0-5 ℃.
Further, the stimulating snail secretes mucus: the snail is placed in a box body to collect mucus, and the box body vibrates to stimulate the snail to secrete the mucus.
A snail facial mask containing snail secretion filtrate, each facial mask contains 30ml facial mask solution containing water, glycerol, propylene glycol, snail secretion filtrate, hydrolyzed vegetable protein, Aloe Barbadensis extract, Concha Margaritifera extract, hyaluronic acid, dipotassium glycyrrhizinate, allantoin, disodium EDTA, and propyl benzoate.
The using method comprises the following steps:
(1) cleaning facial skin, soaking a towel in hot water, wringing to dry, applying to facial skin, and holding for 2-3 min to remove towel;
(2) applying 5-6 drops of essential oil on facial skin, gently massaging for 3-5 min in a manner of making a loop on finger and abdomen, pressing with palm for 20-30 min, and cleaning with warm water.
The preparation method of the snail secretion filtrate comprises the following steps: stimulating snail to secrete mucus by physical method, collecting mucus, centrifuging at high speed to break mucus cell, and collecting supernatant to obtain snail secretion filtrate containing snail active enzyme. It should be noted that during the preparation of the snail secretion filtrate, the temperature of mucus needs to be kept within 0-5 ℃, so that during the centrifugation process, the operation needs to be carried out at minus 3-6 ℃ to avoid the temperature rise during the centrifugation process. Meanwhile, mucus is collected and cannot be refrigerated at low temperature, and partial helicase is easy to lose activity in the processes of refrigeration and subsequent thawing.
Wherein the physical methods include mechanical vibration, electrical stimulation, and the like.
Selecting materials:
glycerol: has the functions of moisture retention and moistening, and also has the special effects of high activity, oxidation resistance, alcoholization promotion and the like.
Propylene glycol: as moisturizer, softener and solvent in cosmetic
Hydrolyzing the vegetable protein: is the product of plant protein hydrolyzed under the action of acid catalysis. The main components of the product are amino acids, and the product has effects of preventing wrinkle, nourishing, retaining water and keeping moisture.
Aloe vera extract: the anthraquinone compounds of Aloe have skin astringing, softening, moisturizing, antiinflammatory, and bleaching effects. It also has effects in relieving sclerosis and keratinization, improving scar, preventing wrinkle, eye pouch, and cutis laxa, keeping skin moist and delicate, treating skin inflammation, and also has good therapeutic effect on acne, freckle, acne, scald, knife wound, insect bite, etc
Mother-of-pearl extract: has skin whitening and moisturizing effects.
Hyaluronic acid: it is also called hyaluronic acid to keep skin moisture, moisten skin and hair, increase luster, and prevent chapped skin and wrinkles.
Dipotassium glycyrrhizinate: the dipotassium glycyrrhizinate has multiple effects of bacteriostasis, inflammation diminishing, detoxification, anti-allergy, deodorization and the like.
Allantoin: the allantoin is an amphoteric compound, can combine with multiple substances to form double salt, has the effects of protecting from light, sterilizing, preventing corrosion, relieving pain, resisting oxidation, and keeping skin moisture, moistening and softening.
Disodium EDTA: a complexing agent is added into cosmetic for complexing some metal ions and stabilizing cosmetic.
Propyl benzoate: a preservative for cosmetics.
Example 2
This example is essentially the same as example 1, except for the method of stimulating the snail to secrete mucus.
Through experimental research, the inventor of the application finds that the snail which is stimulated by mechanical vibration or electrical stimulation and the like can not be directly injured by death, but the state of the snail which is stimulated by mechanical vibration or electrical stimulation is obviously changed, and when the stimulated snail and the normal snail are put together for culturing, the stimulated snail is easily eaten by other normal snails. Therefore, the snail after mechanical vibration or electrical stimulation can not be cultured normally, and the utilization rate of the snail is reduced.
In response to this problem, the present inventors tried other methods to stimulate the snail to secrete mucus. The observation shows that the snail walks on or near the sharp object and easily secretes mucus, and further shows that
The snail secretes mucus easily when seeing a sharp object.
Therefore, the method for stimulating the snail to secrete mucus in the embodiment is as follows:
the snail is placed in a box to collect the mucus. Various pictures with sharp objects are arranged around the box body, or
The box body is made of transparent material, and various objects with sharp parts, such as roses, cactus, thorns and the like, are arranged around the outside of the box body.
The method can stimulate snail to secrete mucus without damaging snail, and the collected snail can be further cultured normally.
Claims (1)
1. A preparation method of snail facial mask containing snail secretion filtrate is characterized in that the components of the facial mask comprise water, glycerin, propylene glycol, snail secretion filtrate, hydrolyzed vegetable protein, aloe barbadensis extract, nacre extract, hyaluronic acid, dipotassium glycyrrhizinate, allantoin, EDTA disodium and propyl benzoate;
the preparation method of the snail secretion filtrate comprises the following steps:
stimulation of snail mucus secretion: the snail is placed in a box body to collect mucus, various pictures with sharp objects are arranged around the box body, or the box body is made of transparent materials, various objects with sharp parts are arranged around the outside of the box body, the snail is stimulated to secrete the mucus under the condition of not damaging the snail, and the mucus is collected;
extracting filtrate: collecting mucus, centrifuging at high speed to break mucus cell, and collecting supernatant to obtain snail secretion filtrate containing snail active enzyme;
the preparation method of the snail mask comprises the following steps:
heating and stirring: mixing water, glycerol, propylene glycol, snail secretion filtrate, hydrolyzed vegetable protein, Aloe Barbadensis Miller extract, Concha Margaritifera extract, hyaluronic acid, dipotassium glycyrrhizinate, allantoin, disodium EDTA, and propyl benzoate at a certain ratio, stirring under vacuum, controlling temperature to raise from room temperature to T1 while stirring, and raising temperature at 0.1-1 deg.C/min;
stirring at constant temperature: stirring for 2-5h at the temperature of T1;
degassing and stirring: degassing the mixed solution, and cooling to T2 temperature;
and (3) continuously stirring: stirring for 0.5-3h at the temperature of T2;
standing and aging: cooling to room temperature, and standing for 12-48 h;
wherein T1 is 60-80 ℃; t2 is 30-40 ℃;
keeping the mucus within 0-5 ℃ in the preparation process of the snail secretion filtrate, and operating at-3 to-6 ℃ in the centrifugation process to avoid temperature rise in the centrifugation process;
the mucus is collected and stored at the temperature of 0-5 ℃.
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| CN111317705B (en) * | 2018-12-17 | 2023-07-25 | 扬州蓝色生物医药科技有限公司 | Snail extract for inhibiting melanin growth and application thereof in preparing daily chemicals |
| CN110339132A (en) * | 2019-08-16 | 2019-10-18 | 宁波范思黎化妆品科技有限公司 | A kind of snail collagen U.S. flesh facial mask |
| CN110840814A (en) * | 2019-12-17 | 2020-02-28 | 广州圆润生物科技有限公司 | Moisturizing mask containing natural petal extract |
| CN112336679A (en) * | 2020-12-14 | 2021-02-09 | 深圳市汇铭斯智能科技有限公司 | Preparation method of black tulip hydrating mask |
| CN115381762B (en) * | 2022-08-31 | 2023-12-22 | 韩佛化妆品(湖州)有限公司 | Preparation method of snail fermentation filtrate, composition containing snail fermentation filtrate and application of snail fermentation filtrate |
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| US20110027221A1 (en) * | 2002-07-01 | 2011-02-03 | Tianjin Sinobiotech Ltd. | Skincare Composition Comprising HSA Fusion Protein, Preparation Method And Uses Thereof |
| CN103860451A (en) * | 2014-04-01 | 2014-06-18 | 广东伊茗药业有限公司 | Functional skin care product containing snail mucus extractive and preparation method thereof |
| CN105496919A (en) * | 2015-12-21 | 2016-04-20 | 太仓张根木生物科技有限公司 | Snail mask with repairing and wrinkle reducing effects |
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| CN104586732B (en) * | 2015-02-16 | 2018-03-06 | 威海瑞佳丽生物科技有限公司 | A kind of method of the snail pituitary extract extraction of active enzyme |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110027221A1 (en) * | 2002-07-01 | 2011-02-03 | Tianjin Sinobiotech Ltd. | Skincare Composition Comprising HSA Fusion Protein, Preparation Method And Uses Thereof |
| CN103860451A (en) * | 2014-04-01 | 2014-06-18 | 广东伊茗药业有限公司 | Functional skin care product containing snail mucus extractive and preparation method thereof |
| CN105496919A (en) * | 2015-12-21 | 2016-04-20 | 太仓张根木生物科技有限公司 | Snail mask with repairing and wrinkle reducing effects |
| CN106047517A (en) * | 2016-05-31 | 2016-10-26 | 太仓协大申泰羊毛衫有限公司 | Woolen sweater washing liquid containing snail extracting solution |
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