CN107198060A - Host of Cordyceps sinensis larvae feeds and preparation method thereof - Google Patents
Host of Cordyceps sinensis larvae feeds and preparation method thereof Download PDFInfo
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- CN107198060A CN107198060A CN201710488893.2A CN201710488893A CN107198060A CN 107198060 A CN107198060 A CN 107198060A CN 201710488893 A CN201710488893 A CN 201710488893A CN 107198060 A CN107198060 A CN 107198060A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
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Abstract
The present invention proposes a kind of host of Cordyceps sinensis larvae feeds.The feed is wrapped up with ester gum; host of Cordyceps sinensis larvae feeds according to embodiments of the present invention; feed is solidified with the host of Cordyceps sinensis larva that ester gum is wrapped to form, mechanization and scale are operated in preparation, turnover, storage, feeding process beneficial to feed；Further the feed includes serpentgrass and Potentilla anserina, can effective stimulus larval feeding there is provided nutritional ingredient needed for larval growth, growth factor needed for supplement larvae development process.
Description
Technical field
The present invention relates to biological field, in particular it relates to host of Cordyceps sinensis larvae feeds, feed producing method
And its method for raising host of Cordyceps sinensis larva.
Background technology
Cordyceps sinensis is sweet, Wen Ping, nontoxic, is famous strengthening by means of tonics medicine, commonly uses meat boiled dinner, has qi-restoratives to be healthy and strong it
Effect.Suitable for the spitting of blood caused by treatment Lung Qi deficiency and deficiency of both the lung and kidney, pulmonary tuberculosis etc. or blood-stained sputum, cough.Shortness of breath, night sweat etc.,
Also there is good curative effect to impotence due to deficiency of the kidney, waist and knee ache etc., the excellent tonic product for the person that is also oldaged physically weak.Pharmacology modern study
As a result in, cordyceps sinensis contains cordycepic acid about 7%, carbohydrate 28.9%, fat about 8.4%, protein about 25%, fat
82.2% is unrighted acid in fat, in addition, still containing vitamin B12, ergot lipidol, hexose alcohol, alkaloid etc..Winter
Worm summer grass has immunoregulation effect, can increase immune system cell, tissue quantity, promote antibody to produce, increase phagocytosis,
Kill cell quantity, strengthen its function, the function of some immunocytes can be turned down again；It is the active material of anticancer, in vitro
With clear and definite suppression, the effect of killing tumor cell；Cellular energy can be improved, is lessened fatigue；Cardiac function can also be adjusted,
Reduce consumption of the heart to oxygen, anti-arrhythmia；Liver function is adjusted, mitigates hepatic injury, anti-hepatic fibrosis；Regulation breathing system
Unite function, with expansion bronchus, relieving asthma, eliminating the phlegm, prevent the effect of pulmonary emphysema；Renal function is adjusted, mitigates toxicant pair
The infringement of kidney；Hematopoiesis function is adjusted, enhancing marrow generates the ability of haemocyte；Reduce the cholesterol and glycerine three in blood
Ester, improves the HDL favourable to human body, mitigates atherosclerosis；In addition, also having directly antiviral, regulation
Central nervous system function, adjusting sexual function etc. are acted on.
Recently, due to the degeneration and artificial excessive excavation of ecological environment, the yield of cordyceps sinensis declines to a great extent, thus, winter worm
The raising of summer grass host larva and the further system for cultivating cordyceps sinensis have much room for improvement.
The content of the invention
The application is that discovery based on inventor to following facts and problem and understanding are made：
The bat moth larva artificial feed shelf-life used is short in the raising of existing bat moth larvae, be unfavorable for mechanization
Operation, at present also in the laboratory rearing stage.
It is contemplated that at least solving one of technical problem in correlation technique to a certain extent.Therefore, the present invention is carried
Gone out the preparation method of a kind of new man-made feeds formula and mucous membrane, beneficial to feed it is fresh-keeping and conjuncted cultivation dish raising in
Carry out feed automation to deliver, improve operating efficiency, so that artificial feeding bat moth larvae energy order cycle goes down, promote people
The cultivation of work cordyceps sinensis.
In the first aspect of the present invention, the present invention proposes a kind of host of Cordyceps sinensis larvae feeds.According to the present invention's
Embodiment, the feed is wrapped up with ester gum.Inventor find, ester gum parcel feed can feed table
Face forms one layer of barrier film, effectively extends to feed breeding cycle from 20 days or so more than 40 days, is raised so as to reduce to change
The probability that the frequency and pathogenic microorganism of material are propagated.The host of Cordyceps sinensis larva being wrapped to form with ester gum, which solidifies, to be raised
Material, beneficial to feed, mechanization and scale are operated in preparation, turnover, storage, feeding process.
Embodiments in accordance with the present invention, above-mentioned feed can further include at least one following additional technical feature：
Embodiments in accordance with the present invention, the feed includes serpentgrass and Potentilla anserina.Inventor passes through screening experiment
It was found that, plateau plant-serpentgrass and Potentilla anserina are added in feed, the annual survival rate of larva, which is significantly stronger than, adds other high
The nutritional ingredient and ratio that former plant, serpentgrass and Potentilla anserina contain relatively are adapted to larval growth demand.According to the present invention
The host of Cordyceps sinensis larvae feeds of embodiment can effective stimulus larval feeding there is provided nutritional ingredient needed for larval growth, mend
Fill growth factor needed for larvae development process.
Embodiments in accordance with the present invention, the serpentgrass and Potentilla anserina are provided in the form of a powder.Inventor
It was found that, serpentgrass and Potentilla anserina are provided in the form of a powder, advantageously in larva take food and nutritional ingredient is in children
Absorption in polypide.
Embodiments in accordance with the present invention, the feed can further include:Agar powder, oatmeal, soy meal, sucrose
And citric acid and distilled water.Inventor has found, adds appropriate oatmeal and soy meal in feed, is not significantly affected by
The growth of larva, and low altitude area auxiliary material oatmeal and soy meal can reduce consumption and then reduction of the plateau plant in feed
Feed cost；Sucrose can further stimulate taking food for larva simultaneously；And citric acid is as natural antiseptic agent, add in formula
Adding citric acid has no side effect to bat moth larval growth, and can effectively extend the average breeding cycle of feed；Agar chemistry knot
Structure is stable, is difficult to decompose utilization by mycelia, fluid nutrient medium effectively can be converted into solid culture as coagulator by agar
Base.
Embodiments in accordance with the present invention, the distilled water based on 100ml, the consumption of the serpentgrass is 5.5-8g, the goose
Suede wither mound dish consumption be 8-12g.Inventor has found that the consumption of serpentgrass is less than 5.5g, then raises by consumption screening experiment
Effect is significantly bad, but higher than after 8g, feeding effect is not significantly increased, in order to protect altitude environment and cost control, matches somebody with somebody
Serpentgrass powder amount ranges are optimized for 5.5~8g in side；Equally, Potentilla anserina is less than 8g, then feeding effect is significantly bad,
But higher than after 12g, feeding effect is not significantly improved, in order to protect Potentilla anserina in altitude environment and cost control, formula
Powder amount ranges are optimized for 8~12g.
The embodiment that you invent according to this, the distilled water based on 100ml, the consumption of the agar powder is 5-10g, the swallow
The consumption of flour is 1.5-3g, and the consumption of the soy meal is 1.2-2g, and the consumption of the sucrose is 2-3g, the citric acid
Consumption be 0.2-0.4g.Inventor is found through experiments that mentioned component can meet the worm summer in winter in above-mentioned amount ranges, both
The demand of careless host's larval growth, again can further effective stimulus larval feeding, effectively extension feed breeding cycle.
In the second aspect of the present invention, the present invention proposes a kind of host of Cordyceps sinensis larvae feeds.According to the present invention's
Embodiment, based on 100ml distilled water, the feed includes：5.5-8g serpentgrass powder, 8-12g Potentilla anserina powder, 5-10g fine jades
Cosmetics, 1.5-3g oatmeals, 1.2-2g soy meals, 2-3g sucrose, 0.2-0.4g citric acids, wherein, the feed is sweet with rosin
Grease is wrapped up.Host of Cordyceps sinensis larvae feeds according to embodiments of the present invention, using plateau larva most eating plateau plant as
It is main, larval feeding is stimulated there is provided nutritional ingredient needed for larval growth, growth factor needed for supplement larvae development process, and be aided with
The common cultivated plant of low altitude area, utilizes feed breeding grub according to embodiments of the present invention, larva annual survival rate %, average pupa
Weight, eggs on average amount are in excellent level.Meanwhile, when the feed of the application is wrapped up with ester gum, feed mouldability
Good, breeding cycle is longer, beneficial to feed mechanized operation in preparation, turnover, storage, feeding process.
In the third aspect of the present invention, the present invention proposes a kind of method for preparing host of Cordyceps sinensis larvae feeds.Root
According to embodiments of the invention, methods described includes：(1) agar powder and sucrose are added into 100ml distilled water, and in 121 DEG C of bars
High pressure steam sterilization 30min under part；(2) serpentgrass powder, Potentilla anserina powder, oatmeal, soy meal are added into step (1) institute
Obtain in solution, when temperature is down to 55 DEG C, add citric acid；(3) mechanical dissection step (2) product in an aseptic environment, it is described
The size of feed particle diameter after segmentation is 1.5cm；(4) ester gum is quickly sprayed onto to the feed after segmentation, the rosin
The temperature of glyceride is 80 DEG C, to obtain the feed for being enclosed with ester gum.The above-mentioned method for preparing feed, operation letter
It is single, and will not being lost in for the nutritional ingredient of raw material in feed is ensure that, feed ingredient is well mixed, feed is clean, Neng Gouman
The hygienic requirements of sufficient larva raising.Prepared feed breeding grub, larva annual survival rate %, average pupa weight, eggs on average amount
It is in excellent level.And ester gum parcel feed is carried out under the conditions of aforesaid operations, ester gum is in feed surface
Film forming is uniform, and feed good moldability, prepared feed breeding cycle is longer, beneficial to man-made feeds preparing, turnover, storage,
Mechanized operation in feeding process.
Embodiments in accordance with the present invention, the above method can further include at least one following additional technical feature：
Embodiments in accordance with the present invention, the consumption of the serpentgrass is 5.5-8g, and the consumption of the Potentilla anserina is 8-
12g, the consumption of the agar powder is 5-10g, and the consumption of the oatmeal is 1.5-3g, and the consumption of the soy meal is 1.2-
2g, the consumption of the sucrose is 2-3g, and the consumption of the citric acid is 0.2-0.4g.Inventor has found, in above-mentioned amount ranges
It is interior, it can both ensure preferable feeding effect, altitude environment and cost control can be effectively protected again, host of Cordyceps sinensis is met
The demand of larval growth, the breeding cycle of effective stimulus larval feeding, effectively extension feed.
In the fourth aspect of the present invention, the present invention proposes a kind of method for raising host of Cordyceps sinensis larva.According to this
The embodiment of invention, methods described is included to the foregoing feed of host of Cordyceps sinensis larva feeding, or noted earlier
Feed prepared by method.Host of Cordyceps sinensis larva, larva year survival are raised using method according to embodiments of the present invention
Rate %, average pupa weight, eggs on average amount are in excellent level.
Embodiments in accordance with the present invention, the host of Cordyceps sinensis larva raising temperature be -2-14 DEG C, humidity be
30%-90%, cleanliness factor is under conditions of 1,000,000 grades.Above-mentioned rearing conditions are young according to 1981 to 2010 in inventor
The of that month average value of plateau and about 205, periphery meteorological station month value meteorological data is hidden, and day and night temperature is (respectively with reference to cold
Warm season historical data) setting, simulate the climatic environment required for larval growth, more suitable host of Cordyceps sinensis larva
Growth.
Upland soil humidity is 45%-65%, plateau in embodiments in accordance with the present invention, host of Cordyceps sinensis larva raising disk
Soil thickness is 0.3-0.6cm.Inventor has found that 45%-65% upland soil humidity can provide the growth of bat moth larvae suitably
Microenvironment, upland soil thick 0.3-0.6cm can meet bat moth larvae and get into the cave habit and activity space, and then wet in upland soil
Spend for 45%-65%, the need for plateau soil thickness can better meet bat moth larval growth for 0.3-0.6cm environment, enter
One step promotes the growth of host of Cordyceps sinensis larva.
According to a particular embodiment of the invention, the raising is carried out in conjuncted cultivation dish, using feeding in feeding process
Material automation is delivered.Operating efficiency thus is effectively increased, artificial feeding bat moth larvae energy order cycle goes down, promoted complete
The cultivation of Fermented Cordyceps.
According to the still another embodiment of the present invention, the conjuncted cultivation dish is made up of 32 (4*8) individual cells, single list
First cultivation dish specification is the long 6cm* high 3cm of wide 4cm*, is capped above.The conjuncted cultivation dish can accomplish independent culture, it is to avoid
Larva baits the propagation closed with cause of disease.
According to the still another embodiment of the present invention, the host of Cordyceps sinensis larva is the bat of character stabilization after domestication
Moth larvae.Bat moth larvae after domestication more adapts to the Ecology system artificially created, and population at individual life cycle tends to be same
Stepization, compares the cultivation that wild larva is particularly suited for Fermented Cordyceps.
Embodiment
Embodiments of the invention are described below in detail, the embodiment is exemplary, it is intended to for explaining the present invention, and
It is not considered as limiting the invention.
Historical climate data used in this research simulation come from China Meteorological Administration of China Meteorological Administration meteorological data
Room is newest《Chinese terrestrial climate data month Value Data collection》, the data set includes the moon value number of national 752 meteorological sites
According to, and quality control is carried out by China Meteorological Administration.Qinghai-Tibet Platean and about 205, periphery have been arranged from the data set
Meteorological station was from the moon value meteorological data of 1981 to 2010, and it is meteorological to choose the mode inputs such as precipitation, temperature, percentage of possible sunshine
The monthly Value Data of parameter, meteorogical phenomena database is set up after arrangement.
This research simulation Qinghai-Tibet Platean carries out larva raising, and temperature, precipitation, percentage of possible sunshine parameter setting take needed for larval growth
Tibetan Plateau Meteorology database this month average value, monthly joins according to clean area temperature of database change, humidity and percentage of possible sunshine
Number, wherein the plateau temperature difference is adjusted according to changes in temperature season daily, i.e. cold season (October~March), and day and night temperature sets 2 DEG C, warm season
(April~September), day and night temperature sets 5 DEG C.
Larva raising condition can be obtained：Temperature controlling range：-2-14℃；Humid control scope：30%-90%；It is clean between raising
Cleanliness requirement：1000000 grades, operating personnel need to dress corresponding clean garment and be tested.
Parameter of the man-made feeds in cultivation dish be：Sterile upland soil humid control is in 45%-65%；Cultivate tray bottom high
Original soil thickness is 0.3-0.6cm.
The preparation of the bat moth larva artificial feed of embodiment 1
Serpentgrass powder, Potentilla anserina powder in the present invention are Qinghai-Tibet bat moth larvae suitable habitat excavation, remove miscellaneous
Matter, fibrous root etc., clear water is cleaned, section, at once in 55 DEG C of dryings, was then crushed 80 mesh and is obtained.Processed using the above method
Succinctly, gained powder can retain nutritional ingredient to greatest extent.
Adopt and prepare feed with the following method：Triangular flask contains 100ml distilled water, agar powder and sucrose is added, in 121 DEG C of conditions
Lower high pressure steam sterilization 30min, the agar solution of heat is poured into beaker, by load weighted serpentgrass powder, Potentilla anserina
Powder, oatmeal, soy meal are poured into beaker, and are constantly stirred with glass bar, when temperature is down to about 55 DEG C, add lemon
Acid, in an aseptic environment first mechanical dissection man-made feeds growth 1.5cm particle diameters, hot melt rosin when then will be cooled to 80 DEG C
Glyceride is quickly sprayed onto on man-made feeds, reaches false set, forms surface and feed group is made into mucous membrane feed.
Wherein, weigh serpentgrass powder 8.0g, Potentilla anserina powder 10g, agar powder 6g, sucrose 2.5g, oatmeal 2.5g,
Soy meal 2.2g, citric acid 0.3g and water 100ml.Feed group A is prepared using the above method.
Weigh serpentgrass powder 7.5g, Potentilla anserina powder 11g, agar powder 6g, sucrose 3g, oatmeal 4g, soy meal 2.7g,
Citric acid 0.3g and water 100ml.Feed group B is prepared using the above method.
Weigh serpentgrass powder 6.5g, Potentilla anserina powder 12g, agar powder 6g, sucrose 2g, oatmeal 3g, soy meal 2.2g,
Citric acid 0.3g and water 100ml.Feed group C is prepared using the above method.
Weigh serpentgrass powder 6.5g, Potentilla anserina powder 12g, agar powder 6g, sucrose 2g, oatmeal 3g, soy meal 2.2g,
Citric acid 0.3g and water 100ml.Film control group D is prepared using the above method but without hot melt ester gum parcel.
Excavate, clean, disinfect after fresh serpentgrass as feed control group E.
Excavate, clean, disinfect after fresh Potentilla anserina as feed control group F.
It is Radix et Rhizoma Rhei 5g, sorbic acid 0.5g, analysis for soybean powder 15g, jade by formula according to open source literature (Wang Hongsheng) report
Ground rice 10g, wheat bran 8g, dusty yeast 5g, agar 10g, water 100ml man-made feeds are used as feed control group G.
According to Liu Xin etc. publication《A kind of host of Cordyceps sinensis larvae feeds and preparation method thereof》Fodder making pair
According to a group H.
Raising of the DIFFERENT FEED of embodiment 2 to bat moth larvae
First, in cultivation dish after layer overlay 0.3cm, humidity is 55% sterile upland soil, for larva moisturizing, will
Seven processing feed groups in embodiment 1 are prepared into 1.5cm particle sizes according to respective method, and single cultivation lattice are put into 10g feedings
Material, a larva is placed per lattice, and raising in 1,000,000 grades of clean areas is placed in after closing the lid.
100 disks are raised in wherein each processing, and each cultivation dish has 4*8 cultivation lattice, changed afterwards once at regular intervals
Sterile culture disk, feed, sterile upland soil, each processing gained pupa are individually weighed, and carry out emergence mating, are calculated female adult and are put down
Equal spawning rate and egg laying amount, each processing correspondence feed breeding cycle of observation.
The raising in three larva periods by a definite date is carried out, the maintenance data of bat moth are as shown in table 1.
Table 1：
As seen from table：
In terms of the average breeding cycle of feed, wrapped up using the ester gum of the present invention, such as experimental group A, B, C, E, F
All there is longer breeding cycle, up to more than 40 days；
In spawning rate and eggs on average in amount, using feed of the present invention, such as A, B, C, D, E, F are significantly high
In other document man-made feeds control group G, H；
In terms of survival rate of larvae：Using feed of the present invention, better than existing feed or quite, feed group A B C
D better than feed group G H；
Average pupa double recipe face：It is suitable with existing feed using feed of the present invention..
Synthesis result can be seen that the feed provided using the present invention and meet the whole growth phase of bat moth larvae, larva
Spawning rate be significantly better than prior art, preferred scheme is more the reduction of the proportioning of plateau natural feed, reduces to plateau ring
The destruction in border, and mucous membrane feed good moldability of the present invention, breeding cycle are longer, beneficial to man-made feeds preparing, turnover, storage,
Mechanized operation in feeding process.
The plateau feed screening of embodiment 3
In this embodiment, the screening of the plateau plant for feed is described in detail in inventor：
Recent study discovery, the different plant roots and stems parts of the section 35 of the main eating of host of Cordyceps sinensis larva about 19 category,
Wherein partial eclipse Polygonum, the plant of Rheum again, motion eccentric, serpentgrass, the Radix et Rhizoma Rhei of Rheum of such as Polygonum, these plants are more
Number is grown in the plateau such as Tibet, Sichuan, Yunnan, Qinghai band, and the distribution of this and host insect is consistent.
Inventor is from larva annual survival rate is higher and motion eccentric of larva partial eclipse, serpentgrass, Radix et Rhizoma Rhei, goose down wither mound
In dish, Dobinea delavayi, their foster worm effect is investigated, as a result as shown in table 2：
Table 2：
| Feed | Motion eccentric | Serpentgrass | Radix et Rhizoma Rhei | Potentilla anserina | Dobinea delavayi |
| Larva annual survival rate % | 41.8 | 53.6 | 37.1 | 47.7 | 22.4 |
By the annual survival rate of serpentgrass and Potentilla anserina breeding grub is significantly stronger than what other plateau plants were made
Feed, shows that nutritional ingredient and ratio that serpentgrass and Potentilla anserina contain relatively are adapted to larval growth demand, therefore inventor
Select serpentgrass and Potentilla anserina as the high ultimate constituent of combination feed, there is provided needed for larval growth to stimulate larval feeding
Nutritional ingredient, growth factor needed for supplement larvae development process.
The screening of other auxiliary materials in the feed formula of embodiment 4 in addition to plateau plant powder
In the present embodiment, inventor describes the sieve of other auxiliary materials in addition to plateau plant powder in feed formula in detail
Choosing：
Inventor devises several groups of check experiments, and the influence whether added to larval growth of different auxiliary material is investigated respectively,
Wherein, oat control group is obtained without oatmeal on the basis of feed group A；Without soybean on the basis of feed group A
Powder, obtains soybean control group；Without sucrose on the basis of feed group A, control sucrose group is obtained；On the basis of feed group A not
Citric acid is added, citric acid control group is obtained
The above control group raising that three larvas by a definite date go through the phase, the maintenance number of bat moth have been subjected to together with feed group A
According to as shown in table 3,
Table 3：
Analytical table 3 is understood, although this programme is based on plateau larva most eating plateau plant serpentgrass and Potentilla anserina,
But low altitude area auxiliary material oatmeal and soy meal are with the addition of simultaneously without influenceing the growth of larva, but can reduce plateau feed
Consumption, playing a part of protection plateau plant and reduces cost.
Sucrose is the carbohydrate that bat moth larvae can be utilized effectively, and addition sucrose has the effect of certain stimulation larval feeding.
Citric acid is natural antiseptic agent, and appropriate addition has no side effect to bat moth larval growth.
The plateau plant powder consumption of embodiment 5 is screened
In the present embodiment, inventor describes the screening of consumption of the plateau plant powder in feed in detail：
Serpentgrass powder dosage optimization：Weigh Potentilla anserina powder 10g, it is agar powder 6g, sucrose 2.5g, oatmeal 2.5g, big
Bean powder 2.2g, citric acid 0.3g, water 100ml and a certain amount of serpentgrass powder carry out the experiment of serpentgrass powder dosage optimization, wherein
Feed breeding grub containing different amounts of serpentgrass powder, the survival rate of larva is as shown in table 4：
Table 4：
| Serpentgrass powder consumption | 4.0 | 4.5 | 5.0 | 5.5 | 6.0 | 6.5 | 7.0 | 7.5 | 8.0 | 8.5 | 9.0 |
| Larva annual survival rate % | 45.8 | 47.4 | 50.6 | 51.2 | 51.3 | 51.9 | 52.1 | 52.5 | 52.8 | 52.8 | 52.9 |
Analytical table 4 is as can be seen that serpentgrass powder is less than 5.5g, then survival rate of larvae is significantly reduced, but is higher than after 8g, larva
Survival rate does not have significant changes, and feeding effect is not significantly improved, therefore for protection altitude environment and the purpose of cost control,
Serpentgrass powder amount ranges are optimized for 5.5~8g in formula.
Potentilla anserina powder dosage optimization：Weigh bulbil powder 8g, agar powder 6g, sucrose 2.5g, oatmeal 2.5g, soy meal
2.2g, citric acid 0.3g, water 100ml and a certain amount of Potentilla anserina powder carry out the experiment of Potentilla anserina powder powder dosage optimization,
Wherein in the feed breeding grub containing different amounts of Potentilla anserina powder, the survival rate of larva is as shown in table 5：
Table 5：
| Potentilla anserina powder consumption | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 |
| Larva annual survival rate % | 42.8 | 44.4 | 47.6 | 51.9 | 52.3 | 52.8 | 52.9 | 53.4 | 53.5 | 53.6 | 53.7 |
Analytical table 5 is as can be seen that Potentilla anserina powder is less than 8g, then feeding effect is significantly bad, but higher than after 12g, raises
Foster effect is not significantly improved, and inventor is in order to protect Potentilla anserina powder consumption model in altitude environment and cost control, formula
Enclose and be optimized for 8~12g.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means to combine specific features, structure, material or the spy that the embodiment or example are described
Point is contained at least one embodiment of the present invention or example.In this manual, to the schematic representation of above-mentioned term not
Identical embodiment or example must be directed to.Moreover, specific features, structure, material or the feature of description can be with office
Combined in an appropriate manner in one or more embodiments or example.In addition, in the case of not conflicting, this area
Technical staff can be carried out the feature of the not be the same as Example described in this specification or example and non-be the same as Example or example
With reference to and combination.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changed, replacing and modification.
Claims (10)
1. a kind of host of Cordyceps sinensis larvae feeds, it is characterised in that the feed is wrapped up with ester gum.
2. feed according to claim 1, it is characterised in that the feed includes：Serpentgrass and Potentilla anserina, preferably
Ground, the serpentgrass and Potentilla anserina are powdered.
3. feed according to claim 2, it is characterised in that further comprise：Agar powder, oatmeal, soy meal, sugarcane
Sugar, citric acid and distilled water.
4. feed according to claim 2, it is characterised in that the distilled water based on 100ml, the consumption of the serpentgrass is
5.5-8g, the consumption of the Potentilla anserina is 8-12g.
5. feed according to claim 3, it is characterised in that the consumption of the agar powder is 5-10g, the oatmeal
Consumption is 1.5-3g, and the consumption of the soy meal is 1.2-2g, and the consumption of the sucrose is 2-3g, and the consumption of the citric acid is
0.2-0.4g。
6. a kind of host of Cordyceps sinensis larvae feeds, it is characterised in that the distilled water based on 100ml, the feed includes：
5.5-8g serpentgrass powder, 8-12g Potentilla anserina powder, 5-10g agar powders, 1.5-3g oatmeals, 1.2-2g soy meals, 2-
3g sucrose, 0.2-0.4g citric acids, wherein, the feed is wrapped up with ester gum.
7. a kind of method for preparing host of Cordyceps sinensis larvae feeds, it is characterised in that including：
(1) agar powder and sucrose, and the high pressure steam sterilization 30min under the conditions of 121 DEG C are added into 100ml distilled water；
(2) serpentgrass powder, Potentilla anserina powder, oatmeal, soy meal are added in step (1) resulting solution, when temperature is down to
At 55 DEG C, citric acid is added；
(3) mechanical dissection step (2) product in an aseptic environment, the size of the feed particle diameter after the segmentation is 1.5cm；
(4) ester gum is quickly sprayed onto to the feed after segmentation, the temperature of the ester gum is 80 DEG C, to obtain
It is enclosed with the feed of ester gum；
Optionally, the consumption of the serpentgrass is 5.5-8g, and the consumption of the Potentilla anserina is 8-12g, the agar powder
Consumption is 5-10g, and the consumption of the oatmeal is 1.5-3g, and the consumption of the soy meal is 1.2-2g, the consumption of the sucrose
For 2-3g, the consumption of the citric acid is 0.2-0.4g.
8. a kind of method for raising host of Cordyceps sinensis larva, it is characterised in that including：
To the feed described in the host of Cordyceps sinensis larva feeding any one of claim 1~6, or side described in claim 7
Feed prepared by method.
9. method according to claim 8, it is characterised in that the host of Cordyceps sinensis larva raising is -2- in temperature
14 DEG C, humidity be that 30%-90%, cleanliness factor are under conditions of 1,000,000 grades.
10. method according to claim 8, it is characterised in that upland soil humidity in host of Cordyceps sinensis larva raising disk
For 45%-65%, plateau soil thickness is 0.3-0.6cm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113854408A (en) * | 2021-10-09 | 2021-12-31 | 重庆市中药研究院 | Artificial feed for cordyceps sinensis host low-age larvae |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103371295A (en) * | 2012-04-19 | 2013-10-30 | 薛刚 | Cordyceps sinensis and hepialus based feed coating material and preparation method thereof and coating feed |
| CN104108980A (en) * | 2014-06-05 | 2014-10-22 | 铜陵市银树生态养殖有限责任公司 | Rosin glyceride-coated fertilizer and preparation method thereof |
| CN106070077A (en) * | 2016-07-02 | 2016-11-09 | 广东东阳光药业有限公司 | The method of host of Cordyceps sinensis larva is raised in a kind of rotation |
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2017
- 2017-06-23 CN CN201710488893.2A patent/CN107198060B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103371295A (en) * | 2012-04-19 | 2013-10-30 | 薛刚 | Cordyceps sinensis and hepialus based feed coating material and preparation method thereof and coating feed |
| CN104108980A (en) * | 2014-06-05 | 2014-10-22 | 铜陵市银树生态养殖有限责任公司 | Rosin glyceride-coated fertilizer and preparation method thereof |
| CN106070077A (en) * | 2016-07-02 | 2016-11-09 | 广东东阳光药业有限公司 | The method of host of Cordyceps sinensis larva is raised in a kind of rotation |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113854408A (en) * | 2021-10-09 | 2021-12-31 | 重庆市中药研究院 | Artificial feed for cordyceps sinensis host low-age larvae |
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