CN104876696A - Tricholorna lobayense culture medium - Google Patents
Tricholorna lobayense culture medium Download PDFInfo
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- CN104876696A CN104876696A CN201510241788.XA CN201510241788A CN104876696A CN 104876696 A CN104876696 A CN 104876696A CN 201510241788 A CN201510241788 A CN 201510241788A CN 104876696 A CN104876696 A CN 104876696A
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- lobayense
- ramulus mori
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- tricholorna
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a tricholorna lobayense culture medium, and relates to the technical field of cultivation. The tricholorna lobayense culture medium is prepared from the following raw materials in parts by weight: 100-150 parts of ramulus mori rods, 100-150 parts of mushroom dregs, 20-30 parts of silkworm excrement, 5-10 parts of a calcium ion solvent, 10-20 parts of quick lime, 20-30 parts of artemisia annua residue powder, 2-5 parts of gypsum, 20-30 parts of rice bran and 350-400 parts of water. Compared with the prior art, according to the adopted culture medium, with the ramulus mori rods and the mushroom dregs as main materials, the ramulus mori rods are easily-available in raw material and low in price; the mushroom dregs are leftovers abandoned by people; the waste is utilized; the cultured tricholorna lobayense and the tricholorna lobayense cultivated by cottonseed hull are the same in nutrient value; the production cost is low; and the growth time is short.
Description
Technical field
The present invention relates to technical field of cultivation, especially a kind of T.lobayense Heim substratum.
Background technology
T.lobayense Heim, be a kind of high-quality edible mushroom be in exploitation, T.lobayense Heim sporophore is grown thickly or is clustered, and shape is large, and white or oyster white, cap is open and flat smooth, in semisphere.Stem is up-small and down-big, and in long bar-shaped, mushroom shape is beautiful, attitude is graceful.T.lobayense Heim bacterial context is plump in vain tender, and mouthfeel is unique, and taste is micro-sweet, tender and crisp and delicious, nutritious.According to analysis, its gross protein value 27%, crude fat 9.5%, total reducing sugar 38.4%, and containing multivitamin.Tasty mouthfeel, shape is beautiful, commodity valency is extra-high.Anti-assorted power is strong, Functionality, quality and appealing design.
At present, T.lobayense Heim artificial culture has raw material, fermentation material and grog three kinds of forms, and because its anti-hybrid ability is strong, mycelial growth is grown fast, and China adopts raw material and fermentation material cultivation mostly.The most frequently used culture material of existing T.lobayense Heim is cotton seed hulls, cotton seed hulls price rises steadily in recent years, the cost of T.lobayense Heim grower is increased, and the price of T.lobayense Heim lags far behind the rise with cost of material, the benefit of peasant's culturing edible fungus reduces greatly, the serious contusion plantation enthusiasm of grower; Find cheap substitute, the Sustainable development of guarantee T.lobayense Heim plant husbandry.
Guangxi is one of maximum province of kind of Sang Yangcan, and ramulus mori bar has hundreds of thousands of ton every year, and the silkworm faeces of the silkworm pull-out of supporting also has several tons; According to surveying and determination, containing multiple nutrients material in ramulus mori bar, as crude protein 5.84%, xylogen 18.2%, Mierocrystalline cellulose 51.5%, ash content 1.6% etc., be suitable as very much the raw materials for production of T.lobayense Heim, and the cost of ramulus mori bar is lower than cotton seed hulls, a kind of trend will be become with ramulus mori bar as the raw materials for production of T.lobayense Heim; In addition, along with the development of mushroom industry, cultivated the tankage of edible mushrooms----bacterium slag also gets more and more, and defines certain pressure to environment.
Summary of the invention
It is low and cultivate the nutritious T.lobayense Heim substratum of T.lobayense Heim out that problem to be solved by this invention is to provide a kind of production cost.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:
Be made up of the raw material of following weight part:
100 parts ~ 150 parts, ramulus mori bar, bacterium slag 100 parts ~ 150 parts, silkworm excrement 20 ~ 30 parts, ionized calcium solvent 5 ~ 10 parts, unslaked lime 10 ~ 20 parts, 20 ~ 30 parts, Herba Artemisiae annuae residue powder, 2 ~ 5 parts, gypsum, 20 ~ 30 parts, rice bran, 350 parts ~ 400 parts, water.
In technique scheme, scheme can also be more specifically: the preparation process of described substratum is:
Step one, ramulus mori bar is dried rear pulverizing, cross 100 mesh sieves, bacterium slag is broken into powder, the ramulus mori bar powder after pulverizing is mixed with bacterium ground-slag, Herba Artemisiae annuae residue powder, rice bran, adds water and ionized calcium stirring solvent;
Step 2, silkworm excrement to be become thoroughly decomposed;
Step 3, step one gains to be mixed with step 2 gains, then add unslaked lime and terra alba, stir;
Step 4, substratum obtained for step 3 is carried out building heap, pile high 1 meter ~ 1.2 meters, wide 1.2 ~ 1.5 meters, then will the superficial compaction of heap, get through pore every 0.5 meter;
When in step 5, measurement heap, 20 centimeters temperature rise to 60 DEG C ~ 70 DEG C, turning, turns evenly by heap with out-pile culture material, when after this turning, when material temperature is warmed up to more than 60 DEG C, is incubated 1 ~ 2 day again, then turning, is total to turning 3 times successively, fermentation ends.
Further: described ionized calcium solvent contains at least one in calcium acetate, citrate of lime, calglucon.
Herba Artemisiae annuae of the present invention, is the dry aerial parts of China traditional drugs artemisia Herba Artemisiae annuae, has another name called sweet wormwood, has clearing away summer heat, sterilization, desinsection, cuts the effects such as cruel, and its principle active component is antimalarial artemisinin and volatilization wet goods.For Artemisinin extraction is Herba Artemisiae annuae branch, leaf.Artemislnin content about about 1.0% in Herba Artemisiae annuae branch, leaf, industrial abstract rate about 90%, after extracting, Herba Artemisiae annuae residue fiber accounts for more than 85%, and crude protein is no less than 8%, can as fiber food; Effective component except residual Artemisinin, also containing abundant flavone component, and other water-soluble traditional Chinese medicine ingredients, can relieving summer-heat, sterilization, desinsection, cut cruel etc.In addition, Herba Artemisiae annuae is Guangxi important Chinese medicine resource, and annual Artemisinin extraction yield accounts for more than 1/3 of national Artemisinin output, this is for the enterprise of Artemisinin annual production 50 tons, nearly 6000 tons of Herba Artemisiae annuae residue, burns as coal surrogate if this part resource is only simple, really belongs to unfortunately.
Bacterium slag of the present invention is the tankage of cultivating edible mushrooms.
The present invention compared with prior art, has following beneficial effect:
1, due in substratum of the present invention with ramulus mori bar and bacterium slag for major ingredient, ramulus mori bar raw material is easy to get, low price, and the tankage that bacterium slag is people to be discarded, utilization of waste material; The T.lobayense Heim nutritive value cultivated out is the same with the T.lobayense Heim nutritive value that cotton seed hulls is cultivated out, and low production cost, growth time is short.
2, be added with ionized calcium solvent in the substratum prepared due to the present invention, make the edible mushrooms planted contain higher calcium contents, add the nutritive ingredient of edible mushrooms; And in basal culture medium, be also added with the residue after with Herba Artemisiae annuae extraction Artemisinin, this residue is except residual Artemisinin, also containing abundant flavone component, and other water-soluble traditional Chinese medicine ingredients, the T.lobayense Heim energy relieving summer-heat making to plant, sterilization, desinsection, cut the effects such as cruel, and the present invention makes full use of Herba Artemisiae annuae residue powder, achieves resource and uses.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.These embodiments are only illustrative, instead of limit the scope of the invention.
Embodiment 1:
The T.lobayense Heim substratum of the present embodiment comprises the steps:
The preparation of A, substratum: adopt the raw material of following weight part to make substratum:
100 parts, ramulus mori bar, bacterium slag 150 parts, silkworm excrement 30 parts, calcium acetate solvent 5 parts, unslaked lime 20 parts, 20 parts, Herba Artemisiae annuae residue powder, 2 parts, gypsum, 30 parts, rice bran, 350 parts, water,
Preparation process is: step one, ramulus mori bar is dried rear pulverizing, crosses 100 mesh sieves, bacterium slag is broken into powder, is mixed by the ramulus mori bar powder after pulverizing, add water and calcium acetate stirring solvent with bacterium ground-slag, Herba Artemisiae annuae residue powder, rice bran; Step 2, silkworm excrement to be become thoroughly decomposed; Step 3, step one gains to be mixed with step 2 gains, then add unslaked lime and terra alba, stir;
B, the substratum of step A gained is carried out building heap, pile high 1 meter, wide 1.2 meters, then will the superficial compaction of heap, get through pore every 0.5 meter;
When in C, measurement heap, 20 centimeters temperature rise to 60 DEG C, turning, turns evenly by heap with out-pile culture material, when after this turning, when material temperature is warmed up to more than 60 DEG C, is incubated 1 day again, then turning, is total to turning 3 times successively, fermentation ends;
D, spread stockpile out, adjust the potential of hydrogen of culture material, make pH value be 7 ~ 8, then pack, then by this culture medium bag as in Autoclave, in 2.5 hours, sterilizing kettle temperature is risen to 130 DEG C, be incubated 1 hour, then stop heating, more vexed 4 hours; Then, after culture medium bag being naturally cooled to 20 DEG C ~ 30 DEG C under the environment of sterilization, aseptic inoculation room is moved to;
E, under the condition of aseptic technique, to be inoculated in the cooling culture material of step D gained by T.lobayense Heim cultivar, more postvaccinal culture medium bag is put into culturing room's lucifuge to cultivate 25 days, in culturing room, temperature is 16 ~ 28 DEG C, and relative air humidity is with 60% ~ 70%;
F, when mycelia purseful, bacterium bag is moved to mushroom room, the bedding according to a conventional method of mushroom room, degree of depth 20cm, one deck lime powder sterilizing pesticide is sprinkled in furrow face and surrounding thereof, then the pocket covering with mycelia is peelled off plastics bag film, bacterium rod vertical setting of types is put into furrow, 3cm space is stayed between bacterium rod, fill up with the loam of disinfecting, thickness of earth covering is 3cm, then spray water, shed is barricaded as again at bacterium rod napex bamboo chip, in order to heat and moisture preserving, in mushroom room, relative air humidity is 85% ~ 90%, temperature is 10 ~ 30 DEG C, and strengthen ventilation, promote that former base is grown, cultivated through 15 days and get final product fruiting, from mushroom flower bud be formed to maturation gather need 5-7 days, when the plump consolidation of cap, mycoderm not yet broken umbrella time gather.
Embodiment 2:
The T.lobayense Heim substratum of the present embodiment comprises the steps:
The preparation of A, substratum: adopt the raw material of following weight part to make substratum:
150 parts, ramulus mori bar, bacterium slag 100 parts, silkworm excrement 20 parts, citrate of lime solvent 10 parts, unslaked lime 10 parts, 30 parts, Herba Artemisiae annuae residue powder, 5 parts, gypsum, 20 parts, rice bran, 400 parts, water,
Preparation process is: step one, ramulus mori bar is dried rear pulverizing, crosses 100 mesh sieves, bacterium slag is broken into powder, is mixed by the ramulus mori bar powder after pulverizing, add water and citrate of lime stirring solvent with bacterium ground-slag, Herba Artemisiae annuae residue powder, rice bran; Step 2, silkworm excrement to be become thoroughly decomposed; Step 3, step one gains to be mixed with step 2 gains, then add unslaked lime and terra alba, stir;
B, the substratum of step A gained is carried out building heap, pile high 1.2 meters, wide 1.5 meters, then will the superficial compaction of heap, get through pore every 0.5 meter;
When in C, measurement heap, 20 centimeters temperature rise to 70 DEG C, turning, turns evenly by heap with out-pile culture material, when after this turning, when material temperature is warmed up to more than 60 DEG C, is incubated 2 days again, then turning, is total to turning 3 times successively, fermentation ends;
D, spread stockpile out, adjust the potential of hydrogen of culture material, make pH value be 8, then pack, then by this culture medium bag as in Autoclave, in 3.5 hours, sterilizing kettle temperature is risen to 130 DEG C, be incubated 2 hours, then stop heating, more vexed 2 hours; Then, after culture medium bag being naturally cooled to 20 DEG C ~ 30 DEG C under the environment of sterilization, aseptic inoculation room is moved to;
E, under the condition of aseptic technique, to be inoculated in the cooling culture material of step D gained by T.lobayense Heim cultivar, more postvaccinal culture medium bag is put into culturing room's lucifuge to cultivate 30 days, in culturing room, temperature is 16 ~ 28 DEG C, and relative air humidity is with 60% ~ 70%;
F, when mycelia purseful, bacterium bag is moved to mushroom room, the bedding according to a conventional method of mushroom room, degree of depth 20cm, one deck lime powder sterilizing pesticide is sprinkled in furrow face and surrounding thereof, then the pocket covering with mycelia is peelled off plastics bag film, bacterium rod vertical setting of types is put into furrow, 5cm space is stayed between bacterium rod, fill up with the loam of disinfecting, thickness of earth covering is 3cm, then spray water, shed is barricaded as again at bacterium rod napex bamboo chip, in order to heat and moisture preserving, in mushroom room, relative air humidity is 85% ~ 90%, temperature is 10 ~ 30 DEG C, and strengthen ventilation, promote that former base is grown, cultivated through 20 days and get final product fruiting, from mushroom flower bud be formed to maturation gather need 5-7 days, when the plump consolidation of cap, mycoderm not yet broken umbrella time gather.
Claims (3)
1. a T.lobayense Heim substratum, is characterized in that being made up of the raw material of following weight part:
100 parts ~ 150 parts, ramulus mori bar, bacterium slag 100 parts ~ 150 parts, silkworm excrement 20 ~ 30 parts, ionized calcium solvent 5 ~ 10 parts, unslaked lime 10 ~ 20 parts, 20 ~ 30 parts, Herba Artemisiae annuae residue powder, 2 ~ 5 parts, gypsum, 20 ~ 30 parts, rice bran, 350 parts ~ 400 parts, water.
2. T.lobayense Heim substratum according to claim 1, is characterized in that: the preparation process of described substratum is:
Step one, ramulus mori bar is dried rear pulverizing, cross 100 mesh sieves, bacterium slag is broken into powder, the ramulus mori bar powder after pulverizing is mixed with bacterium ground-slag, Herba Artemisiae annuae residue powder, rice bran, adds water and ionized calcium stirring solvent;
Step 2, silkworm excrement to be become thoroughly decomposed;
Step 3, step one gains to be mixed with step 2 gains, then add unslaked lime and terra alba, stir;
Step 4, substratum obtained for step 3 is carried out building heap, pile high 1 meter ~ 1.2 meters, wide 1.2 ~ 1.5 meters, then will the superficial compaction of heap, get through pore every 0.5 meter;
When in step 5, measurement heap, 20 centimeters temperature rise to 60 DEG C ~ 70 DEG C, turning, turns evenly by heap with out-pile culture material, when after this turning, when material temperature is warmed up to more than 60 DEG C, is incubated 1 ~ 2 day again, then turning, is total to turning 3 times successively, fermentation ends.
3. T.lobayense Heim substratum according to claim 1 and 2, is characterized in that: described ionized calcium solvent contains at least one in calcium acetate, citrate of lime, calglucon.
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| CN201510241788.XA CN104876696A (en) | 2015-05-13 | 2015-05-13 | Tricholorna lobayense culture medium |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107382415A (en) * | 2017-07-28 | 2017-11-24 | 林燕 | A kind of culture medium of edible fungus and preparation method thereof |
| CN109275504A (en) * | 2018-11-14 | 2019-01-29 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of culture medium of edible fungus and preparation method thereof containing decomposed silkworm excrement |
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| CN101496485A (en) * | 2009-03-16 | 2009-08-05 | 东南大学 | Application of silkworm and mulberry by-product silkworm excrement fermentation wastes in edible fungus cultivation |
| CN102210233A (en) * | 2011-03-31 | 2011-10-12 | 上海高榕食品有限公司 | Production method of calcium-enriched edible fungi and formula of culture medium |
| CN103145509A (en) * | 2013-04-11 | 2013-06-12 | 曾品涛 | Edible fungus culture medium using sweet wormwood waste residue as main material and preparation method of edible fungus culture medium |
| CN103493686A (en) * | 2013-10-14 | 2014-01-08 | 横县富华农业科技发展有限责任公司 | Method for cultivating tricholoma lobayense heim through mulberry twig stems and cassava stems |
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2015
- 2015-05-13 CN CN201510241788.XA patent/CN104876696A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101496485A (en) * | 2009-03-16 | 2009-08-05 | 东南大学 | Application of silkworm and mulberry by-product silkworm excrement fermentation wastes in edible fungus cultivation |
| CN102210233A (en) * | 2011-03-31 | 2011-10-12 | 上海高榕食品有限公司 | Production method of calcium-enriched edible fungi and formula of culture medium |
| CN103145509A (en) * | 2013-04-11 | 2013-06-12 | 曾品涛 | Edible fungus culture medium using sweet wormwood waste residue as main material and preparation method of edible fungus culture medium |
| CN103493686A (en) * | 2013-10-14 | 2014-01-08 | 横县富华农业科技发展有限责任公司 | Method for cultivating tricholoma lobayense heim through mulberry twig stems and cassava stems |
Non-Patent Citations (1)
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| 周谦群等: "金福菇层架高产栽培技术", 《浙江食用菌》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107382415A (en) * | 2017-07-28 | 2017-11-24 | 林燕 | A kind of culture medium of edible fungus and preparation method thereof |
| CN109275504A (en) * | 2018-11-14 | 2019-01-29 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of culture medium of edible fungus and preparation method thereof containing decomposed silkworm excrement |
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Application publication date: 20150902 |