CN107184834A - Antidepression health products containing rice husk flavones - Google Patents
Antidepression health products containing rice husk flavones Download PDFInfo
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- CN107184834A CN107184834A CN201710363511.3A CN201710363511A CN107184834A CN 107184834 A CN107184834 A CN 107184834A CN 201710363511 A CN201710363511 A CN 201710363511A CN 107184834 A CN107184834 A CN 107184834A
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 63
- 235000009566 rice Nutrition 0.000 title claims abstract description 63
- 239000010903 husk Substances 0.000 title claims abstract description 61
- 229930003944 flavone Natural products 0.000 title claims abstract description 56
- 235000011949 flavones Nutrition 0.000 title claims abstract description 56
- 150000002213 flavones Chemical class 0.000 title claims abstract description 45
- 230000036541 health Effects 0.000 title claims abstract description 27
- 240000007594 Oryza sativa Species 0.000 title 1
- 239000000284 extract Substances 0.000 claims abstract description 72
- 241000209094 Oryza Species 0.000 claims abstract description 62
- 235000008599 Poria cocos Nutrition 0.000 claims abstract description 18
- 235000001188 Peltandra virginica Nutrition 0.000 claims abstract description 11
- 239000010271 massa medicata fermentata Substances 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 48
- 239000000843 powder Substances 0.000 claims description 38
- 238000000605 extraction Methods 0.000 claims description 32
- 239000007787 solid Substances 0.000 claims description 26
- 238000002360 preparation method Methods 0.000 claims description 19
- 238000011045 prefiltration Methods 0.000 claims description 18
- 238000000108 ultra-filtration Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 244000197580 Poria cocos Species 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 15
- 238000002525 ultrasonication Methods 0.000 claims description 15
- 108010059892 Cellulase Proteins 0.000 claims description 13
- 229940106157 cellulase Drugs 0.000 claims description 13
- 239000013049 sediment Substances 0.000 claims description 12
- 239000008367 deionised water Substances 0.000 claims description 10
- 229910021641 deionized water Inorganic materials 0.000 claims description 10
- 238000002137 ultrasound extraction Methods 0.000 claims description 10
- 230000015556 catabolic process Effects 0.000 claims description 9
- 238000006731 degradation reaction Methods 0.000 claims description 9
- 241000132012 Atractylodes Species 0.000 claims description 7
- 244000131316 Panax pseudoginseng Species 0.000 claims description 7
- 235000003181 Panax pseudoginseng Nutrition 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 3
- 238000000967 suction filtration Methods 0.000 claims description 3
- 238000003809 water extraction Methods 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 2
- 238000002386 leaching Methods 0.000 claims description 2
- 238000004108 freeze drying Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 abstract description 17
- 239000003814 drug Substances 0.000 abstract description 12
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 abstract description 11
- 150000002212 flavone derivatives Chemical class 0.000 abstract description 11
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 10
- 230000001430 anti-depressive effect Effects 0.000 abstract description 8
- 239000000935 antidepressant agent Substances 0.000 abstract description 6
- 229940005513 antidepressants Drugs 0.000 abstract description 6
- 230000000994 depressogenic effect Effects 0.000 abstract description 4
- 241000411851 herbal medicine Species 0.000 abstract description 4
- 230000001681 protective effect Effects 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 2
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- 230000002588 toxic effect Effects 0.000 abstract description 2
- 244000248825 Peltandra virginica Species 0.000 abstract 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 18
- 239000000047 product Substances 0.000 description 18
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 15
- 230000006378 damage Effects 0.000 description 10
- 229960002989 glutamic acid Drugs 0.000 description 8
- 235000013922 glutamic acid Nutrition 0.000 description 8
- 239000004220 glutamic acid Substances 0.000 description 8
- 208000027418 Wounds and injury Diseases 0.000 description 7
- 208000014674 injury Diseases 0.000 description 7
- 238000002242 deionisation method Methods 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 206010057248 Cell death Diseases 0.000 description 2
- GIYXAJPCNFJEHY-UHFFFAOYSA-N N-methyl-3-phenyl-3-[4-(trifluoromethyl)phenoxy]-1-propanamine hydrochloride (1:1) Chemical group Cl.C=1C=CC=CC=1C(CCNC)OC1=CC=C(C(F)(F)F)C=C1 GIYXAJPCNFJEHY-UHFFFAOYSA-N 0.000 description 2
- 238000010306 acid treatment Methods 0.000 description 2
- 239000011149 active material Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 230000030833 cell death Effects 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 229960000389 fluoxetine hydrochloride Drugs 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000020925 Bipolar disease Diseases 0.000 description 1
- 206010010144 Completed suicide Diseases 0.000 description 1
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- 241000406668 Loxodonta cyclotis Species 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
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- 208000028683 bipolar I disease Diseases 0.000 description 1
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- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 229940049906 glutamate Drugs 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- 229960003707 glutamic acid hydrochloride Drugs 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
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- 230000016507 interphase Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- 231100000252 nontoxic Toxicity 0.000 description 1
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- 238000011017 operating method Methods 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
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- 208000024891 symptom Diseases 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Classifications
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A61K2236/50—Methods involving additional extraction steps
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- A61K2236/50—Methods involving additional extraction steps
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Abstract
The present invention discloses a kind of antidepression health products containing rice husk flavones, is made up of following raw materials by weight:Rice husk flavones 50 70%, Rhizoma Cyperi extract 5 10%, Rhizoma Atractylodis Macrocephalae extract 5 10%, tuckahoe extracts 5 10%, Notogineng Extract 5 10%, Massa Medicata Fermentata extract 5 10%.Initiative technology of the invention is that, as antidepressant core functionality raw material, the Chinese medicine composition for being used to treat depression of proposition, compatibility is reasonable, and being formed has antidepressant flavones, Chinese herbal medicine mixed health product using rice husk flavones.The present invention passes through the experimental study to external depressed cell model; it was found that the composition of rice husk flavones has preferable protective effect to cell; there is the effect of highly significant for depressed preventing and treating; the toxic side effect that excessive chemical synthetic drug can be avoided to cause; and it is cheap; wide material sources, also cause rice husk flavone composition to receive significant attention.
Description
Technical field
The invention belongs to field of health care products, and in particular to a kind of antidepression health products containing rice husk flavones.
Background technology
Depression is a kind of mode of onset of manic-depressive psychosis, is subtracted with the action of hypothymergasia, retardation of thinking and speech
Few, slow is classical symptom.Depression seriously perplexs the live and work of patient, and white elephant is brought to family and society,
About 15% patients with depression dies from suicide.Depression allows global 3.4 hundred million people's sup sorrows as global 4th big disease, and
This numeral is still rising year by year.The treatment of current depression is main based on Western medicine, but long-time is taken this kind of medicine and can drawn
Serious side effects and adverse reaction are played, prevents patient from obtaining comprehensive, effective treatment.Thus, from natural drug and prescription
Preferable antidepressant is found in agent, increasingly causes the concern of researcher.Domestic and foreign scholars are found that many tools so far
There is the Chinese herbal medicine of antidepressant effect, and part herbal medicine is developed to treat depression.Chinese medicine compound prescription antidepressant effect is
By the result of each composition interphase interaction, due to Chinese medicine compound prescription complicated component, and the pathogenesis of depression is not yet completely clear
It is clear, therefore the clear compound Chinese medicinal preparation of neither one determined curative effect, the mechanism of action is gone back in the market, this also turns into modern section
Worker's urgent problem to be solved, while Chinese medicine compound prescription in terms for the treatment of depression before having greater advantage and applications well
Scape.
The content of the invention
It is an object of the present invention to provide a kind of natural health-care products containing rice husk flavones with antidepression function, the health products
It is that the processing byproduct rice husk of the grass family cereal crops paddy rice planted extensively with China sets out, prepares in rice husk flavones and other
Medicament extract is mixed.
The technical solution adopted by the present invention is:
Antidepression health products containing rice husk flavones, are made up of following raw materials by weight:
Rice husk flavones 50-70%, Rhizoma Cyperi extract 5-10%, Rhizoma Atractylodis Macrocephalae extract 5-10%,
Tuckahoe extracts 5-10%, Notogineng Extract 5-10%, Massa Medicata Fermentata extract 5-10%.
The antidepression health products containing rice husk flavones, the preparation method of the rice husk flavones is as follows:
1) cellulase degradation is extracted:Solid-liquid ratio in mass ratio 1:10-1:30, rice husk powder is dissolved in water, cellulase
Addition is 120-180U/g, carries out hot water extraction, and it is 50-60 DEG C to control temperature, pH value range 5.0-6.0, and enzymolysis time is
1-2h, is centrifuged, and filtering obtains that cellulase degradation extract solution is standby, sediment A proceeds ethanol assisted extraction;
2) ethanol assisted extraction:Solid-liquid ratio in mass ratio 1:10-1:30, sediment A is dissolved in ethanol, volume fraction of ethanol
For 50-70%, extraction time is 2-3h, is centrifuged, and filtering obtains that ethanol assisted extraction liquid is standby, sediment B proceeds ultrasonic leaching
Carry;
3) ultrasound extraction:Solid-liquid ratio in mass ratio 1:10-1:30, sediment B is dissolved in ethanol, volume fraction of ethanol is
50-70%, ultrasonic power is 160-200W, and ultrasonic time is 20-30min, and suction filtration obtains ultrasonic extraction extract solution standby;
4) merge cellulase degradation extract solution, ethanol assisted extraction liquid and ultrasound extraction extract solution, total extract solution is revolved
Turn to be concentrated by evaporation, after filter paper prefiltration, ultrafiltration is carried out with filter membrane, ultrafiltrate sterile cryo is dried, and obtains thick rice husk flavones dry powder.
The antidepression health products containing rice husk flavones, the preparation method of the Rhizoma Cyperi extract is:In room temperature condition
Under, weigh the rhizoma cyperi solid powder for being ground into 200 mesh;Difference in mass ratio 1:10 ratios weigh rhizoma cyperi solid powder and deionization
Water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate sterile cryo is dried, obtains rhizoma cyperi and carry
Take thing.
The antidepression health products containing rice husk flavones, the preparation method of the Rhizoma Atractylodis Macrocephalae extract is:In room temperature condition
Under, weigh the bighead atractylodes rhizome solid powder for being ground into 200 mesh;Difference in mass ratio 1:10 ratios weigh bighead atractylodes rhizome solid powder and deionization
Water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate sterile cryo is dried, obtains the bighead atractylodes rhizome and carry
Take thing.
The antidepression health products containing rice husk flavones, the preparation method of the tuckahoe extracts is:In room temperature condition
Under, weigh the Poria cocos solid powder for being ground into 200 mesh;Difference in mass ratio 1:10 ratios weigh Poria cocos solid powder and deionization
Water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate sterile cryo is dried, obtains Poria cocos and carry
Take thing.
The antidepression health products containing rice husk flavones, the preparation method of the Notogineng Extract is:In room temperature condition
Under, weigh the pseudo-ginseng solid powder for being ground into 200 mesh;Difference in mass ratio 1:10 ratios weigh pseudo-ginseng solid powder and deionization
Water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate sterile cryo is dried, obtains pseudo-ginseng and carry
Take thing.
The antidepression health products containing rice husk flavones, the preparation method of the Massa Medicata Fermentata extract is:In room temperature condition
Under, weigh the Divine Comedy solid powder for being ground into 200 mesh;Difference in mass ratio 1:10 ratios weigh Divine Comedy solid powder and deionization
Water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate sterile cryo is dried, obtains Divine Comedy and carry
Take thing.
The beneficial effects of the invention are as follows:
Initiative technology of the invention is, as antidepressant core functionality raw material, to propose to be used to treat using rice husk flavones
Depressed Chinese medicine composition, rationally, formed has antidepressant rice husk flavones, Chinese herbal medicine mixed health product to compatibility.The present invention is logical
The experimental study to external Glu-induced Injury cell is crossed, finds the composition of rice husk flavones to external Glu-induced Injury PC12 cells
With good inhibiting effect, there is the effect of highly significant for depressed preventing and treating, excessive chemical synthetic drug can be avoided
The toxic side effect that thing is caused, and cheap, wide material sources, also cause the rice husk flavone composition to receive significant attention.
The flavones that the present invention is extracted using paddy rice processing byproduct rice husk powder is as active material, and materials are easy, compared with
Preferable extraction effect can be reached under conditions of gentle, operating method is simple, and extraction time is short, easy to operate, extract activity
The nontoxic secondary use of height, end-product and intermediate product, and it is easy to carry, and mouthfeel is balanced, beneficial to absorption of human body, is easy to high-volume raw
Production, with promotional value.
Brief description of the drawings
Fig. 1 is that various concentrations glutamic acid influences on PC12 cell strain growths.
Fig. 2 is the protective effect of various concentrations rice husk flavone composition Glu-induced Injury PC12 cells.
Embodiment
Antidepression health products of the embodiment 1 containing rice husk flavones
(1) preparation of rice husk flavones
Rice husk flavones is extracted using the process combination of cellulase degradation-alcohol steep-ultrasonic wave method of fractional steps, step is such as
Under:
1) cellulase degradation is extracted:Rice husk is gone into the removal of impurity, dried, size-reduced machine is crushed, 60 mesh sieves is crossed, rice husk is made
Powder.Solid-liquid ratio in mass ratio 1:30, rice husk powder is dissolved in water, cellulase addition is 150U/g, carries out hot water extraction,
It is 50 DEG C to control temperature, pH 5.0, and enzymolysis time is 2h, and 4000r/min carries out centrifugation 15min, and filtering obtains cellulase degradation
Extract solution is standby, and sediment A proceeds ethanol assisted extraction;
2) ethanol assisted extraction:Solid-liquid ratio in mass ratio 1:20, sediment A is dissolved in ethanol, volume fraction of ethanol is
60%, extraction time is 3h, is centrifuged, and filtering obtains that ethanol assisted extraction liquid is standby, sediment B proceeds ultrasonic extraction;
3) ultrasound extraction:Solid-liquid ratio in mass ratio 1:20, sediment B is dissolved in ethanol, volume fraction of ethanol is 60%, surpassed
Acoustic power is 180W, and ultrasonic time is 30min, and suction filtration obtains ultrasonic extraction extract solution standby;
4) merge cellulase degradation extract solution, ethanol assisted extraction liquid and ultrasound extraction extract solution, total extract solution is revolved
Turn to be concentrated by evaporation, after filter paper prefiltration, ultrafiltration is carried out with filter membrane, ultrafiltrate sterile cryo is dried, and obtains thick rice husk flavones dry powder.
(2) preparation of Rhizoma Cyperi extract
At ambient temperature, the rhizoma cyperi solid powder for being ground into 200 mesh is weighed;In mass ratio 1:10 ratios weigh rhizoma cyperi and consolidated
Body powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate are sterile cold
It is lyophilized dry, obtain Rhizoma Cyperi extract.
(3) preparation of Rhizoma Atractylodis Macrocephalae extract
At ambient temperature, the bighead atractylodes rhizome solid powder for being ground into 200 mesh is weighed;In mass ratio 1:10 ratios weigh the bighead atractylodes rhizome and consolidated
Body powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate are sterile cold
It is lyophilized dry, obtain Rhizoma Atractylodis Macrocephalae extract.
(4) preparation of tuckahoe extracts
At ambient temperature, the Poria cocos solid powder for being ground into 200 mesh is weighed;In mass ratio 1:10 ratios weigh Poria cocos and consolidated
Body powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate are sterile cold
It is lyophilized dry, obtain tuckahoe extracts.
(5) preparation of Notogineng Extract
At ambient temperature, the pseudo-ginseng solid powder for being ground into 200 mesh is weighed;In mass ratio 1:10 ratios weigh pseudo-ginseng and consolidated
Body powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate are sterile cold
It is lyophilized dry, obtain Notogineng Extract.
(6) preparation of Massa Medicata Fermentata extract
At ambient temperature, the Divine Comedy solid powder for being ground into 200 mesh is weighed;Difference in mass ratio 1:10 ratios weigh god
Bent solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate without
Bacterium is freeze-dried, and obtains Massa Medicata Fermentata extract.
(7) antidepression Halth-care composition (abbreviation rice husk flavone composition) containing rice husk flavones
It is made up of following raw materials by weight:
Rice husk flavones 60%, Rhizoma Cyperi extract 5%, Rhizoma Atractylodis Macrocephalae extract 10%,
Tuckahoe extracts 5%, Notogineng Extract 10%, Massa Medicata Fermentata extract 10%
(8) MTT experiment
The present embodiment uses Glu-induced Injury cell line PC12 cells, detects that rice husk flavone composition is external using mtt assay
Antidepressant activity, experimental procedure is as follows:
1) take the logarithm the PC12 cells in growth period, with cell number 3 × 104/ m L are inoculated in 96 well culture plates (100 μ L/
Hole), cellar culture 24h makes its adherent.
2) concentration (3.125,6.25 and 12.5mg/ml) of rice husk flavone composition;Glutamic acid treatment group concentration (5,
10th, 15 and 20mmol/L)
3) cell is divided into Normal group, glutamic acid treatment group, positive controls and rice husk flavone composition to pre-process
Damage group afterwards.Normal group, is routinely added to nutrient solution;Glutamate induction damage group, while adding nutrient solution and glutamic acid;Sun
Property control group, add glutamic acid and Fluoxetine hydrochloride;Medicament protection group:12h adds the pretreatment of rice husk flavone composition in advance, it
It is synchronous with other groups again afterwards to add Glu-induced Injury.
4) MTT (5mg/ml) is added after 24 hours;
5) and then 96 orifice plates are placed in 37 DEG C of constant incubators and continue to cultivate 4h;
6) after processing terminates, MTT is suctioned out, 150 μ l DMSO is added, is completely dissolved first a ceremonial jade-ladle, used in libation crystal;
7) increment of PC12 cells is assessed by measuring absorbance of each hole at 490nm;As a result as shown in Figure 1, Figure 2
It is shown.
As shown in figure 1, the glutamic acid of various concentrations is incubated PC12 cell 24h respectively, MTT colorimetric determinations are then utilized.
As a result show, after handling cell for a period of time, concentration significantly makes PC12 cell deaths for 15 and 20mM/L glutamic acid.As a result table
Bright, glutamic acid can promote PC12 cell deaths in vitro, and we select 15mM/L to enter as the final concentration of Glu-induced Injury
Row experiment.
As shown in Fig. 2 the rice husk flavone composition of various concentrations is incubated PC12 cell 12h respectively, afterwards plus glutamic acid
Damage, then utilizes MTT colorimetric determinations.Fluoxetine hydrochloride is used as positive control.As a result show, processing cell is for a period of time
Afterwards, concentration shows significantly to promote cell growth effect for 3.125,6.25 and 12.5mg/ml rice husk flavone composition.
As a result show, rice husk flavone composition has certain protective effect to Glu-induced Injury PC12 cells.
Antidepression health products of the embodiment 2 containing rice husk flavones
(1)-(six) be the same as Example 1.
(7) the antidepression health products containing rice husk flavones, are made up of following raw materials by weight:
Rice husk flavones 70%, Rhizoma Cyperi extract 10%, Rhizoma Atractylodis Macrocephalae extract 5%,
Tuckahoe extracts 5%, Notogineng Extract 5%, Massa Medicata Fermentata extract 5%.
Antidepression health products of the embodiment 3 containing rice husk flavones
(1)-(six) be the same as Example 1.
(7) the antidepression health products containing Rice Bran Flavonoid, are made up of following raw materials by weight:
Rice husk flavones 50%, Rhizoma Cyperi extract 10%, Rhizoma Atractylodis Macrocephalae extract 10%,
Tuckahoe extracts 10%, Notogineng Extract 10%, Massa Medicata Fermentata extract 10%.
Claims (7)
1. the antidepression health products containing rice husk flavones, it is characterised in that be made up of following raw materials by weight:
Rice husk flavones 50-70%, Rhizoma Cyperi extract 5-10%, Rhizoma Atractylodis Macrocephalae extract 5-10%,
Tuckahoe extracts 5-10%, Notogineng Extract 5-10%, Massa Medicata Fermentata extract 5-10%.
2. the antidepression health products as claimed in claim 1 containing rice husk flavones, it is characterised in that the preparation of the rice husk flavones
Method is as follows:
1) cellulase degradation is extracted:Solid-liquid ratio in mass ratio 1:10-1:30, rice husk powder is dissolved in water, cellulase is added
Measure as 120-180U/g, carry out hot water extraction, it is 50-60 DEG C control temperature, pH value range 5.0-6.0, and enzymolysis time is 1-2h,
Centrifugation, filtering, obtains that cellulase degradation extract solution is standby, and sediment A proceeds ethanol assisted extraction;
2) ethanol assisted extraction:Solid-liquid ratio in mass ratio 1:10-1:30, sediment A is dissolved in ethanol, volume fraction of ethanol is
50-70%, extraction time is 2-3h, is centrifuged, and filtering obtains that ethanol assisted extraction liquid is standby, sediment B proceeds ultrasonic leaching
Carry;
3) ultrasound extraction:Solid-liquid ratio in mass ratio 1:10-1:30, sediment B is dissolved in ethanol, volume fraction of ethanol is 50-
70%, ultrasonic power is 160-200W, and ultrasonic time is 20-30min, and suction filtration obtains ultrasonic extraction extract solution standby;
4) merge cellulase degradation extract solution, ethanol assisted extraction liquid and ultrasound extraction extract solution, total extract solution is rotated and steamed
After hair concentration, filter paper prefiltration, ultrafiltration is carried out with filter membrane, ultrafiltrate sterile cryo is dried, and obtains thick rice husk flavones dry powder.
3. the antidepression health products as claimed in claim 1 containing rice husk flavones, it is characterised in that the system of the Rhizoma Cyperi extract
Preparation Method is:At ambient temperature, the rhizoma cyperi solid powder for being ground into 200 mesh is weighed;Difference in mass ratio 1:10 ratios are weighed
Rhizoma cyperi solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate
Sterile cryo is dried, and obtains Rhizoma Cyperi extract.
4. the antidepression health products as claimed in claim 1 containing rice husk flavones, it is characterised in that the system of the Rhizoma Atractylodis Macrocephalae extract
Preparation Method is:At ambient temperature, the bighead atractylodes rhizome solid powder for being ground into 200 mesh is weighed;Difference in mass ratio 1:10 ratios are weighed
Bighead atractylodes rhizome solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate
Sterile cryo is dried, and obtains Rhizoma Atractylodis Macrocephalae extract.
5. the antidepression health products as claimed in claim 1 containing rice husk flavones, it is characterised in that the system of the tuckahoe extracts
Preparation Method is:At ambient temperature, the Poria cocos solid powder for being ground into 200 mesh is weighed;Difference in mass ratio 1:10 ratios are weighed
Poria cocos solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate
Sterile cryo is dried, and obtains tuckahoe extracts.
6. the antidepression health products as claimed in claim 1 containing rice husk flavones, it is characterised in that the preparation side of Notogineng Extract
Method is:At ambient temperature, the pseudo-ginseng solid powder for being ground into 200 mesh is weighed;Difference in mass ratio 1:10 ratios weigh pseudo-ginseng
Solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate are sterile
Freeze-drying, obtains Notogineng Extract.
7. the antidepression health products as claimed in claim 1 containing rice husk flavones, it is characterised in that the system of the Massa Medicata Fermentata extract
Preparation Method is:At ambient temperature, the Divine Comedy solid powder for being ground into 200 mesh is weighed;Difference in mass ratio 1:10 ratios are weighed
Divine Comedy solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate
Sterile cryo is dried, and obtains Massa Medicata Fermentata extract.
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Citations (1)
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CN103561755A (en) * | 2011-05-27 | 2014-02-05 | 韩国食品研究院 | Novel usage of rice, rice bran, or chaff extract as histamine receptor antagonist |
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2017
- 2017-05-22 CN CN201710363511.3A patent/CN107184834A/en active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103561755A (en) * | 2011-05-27 | 2014-02-05 | 韩国食品研究院 | Novel usage of rice, rice bran, or chaff extract as histamine receptor antagonist |
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Title |
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关海宁 等: "纤维素酶预处理对稻壳黄酮提取的影响", 《粮食与油脂》 * |
宗春燕等: "正交实验法优选超声辅助提取稻壳中黄酮的工艺", 《安徽化工》 * |
梅全喜: "《现代中药药理与临床应用手册》", 31 October 2016, 中国中医药出版社 * |
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