CN106983830A - Antidepression health products containing Rice Bran Flavonoid - Google Patents
Antidepression health products containing Rice Bran Flavonoid Download PDFInfo
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- CN106983830A CN106983830A CN201710362756.4A CN201710362756A CN106983830A CN 106983830 A CN106983830 A CN 106983830A CN 201710362756 A CN201710362756 A CN 201710362756A CN 106983830 A CN106983830 A CN 106983830A
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- 229930003935 flavonoid Natural products 0.000 title claims abstract description 51
- 150000002215 flavonoids Chemical class 0.000 title claims abstract description 51
- 235000017173 flavonoids Nutrition 0.000 title claims abstract description 51
- 230000036541 health Effects 0.000 title claims abstract description 30
- 239000000284 extract Substances 0.000 claims abstract description 53
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 22
- 241000218378 Magnolia Species 0.000 claims abstract description 18
- 235000005340 Asparagus officinalis Nutrition 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 229930003944 flavone Natural products 0.000 claims abstract description 6
- 150000002213 flavones Chemical class 0.000 claims abstract description 6
- 235000011949 flavones Nutrition 0.000 claims abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 47
- 239000000843 powder Substances 0.000 claims description 37
- 238000000605 extraction Methods 0.000 claims description 31
- 239000007787 solid Substances 0.000 claims description 28
- 238000002360 preparation method Methods 0.000 claims description 19
- 238000011045 prefiltration Methods 0.000 claims description 18
- 238000000108 ultra-filtration Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 241000234427 Asparagus Species 0.000 claims description 16
- 241000209020 Cornus Species 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 15
- 239000008367 deionised water Substances 0.000 claims description 14
- 229910021641 deionized water Inorganic materials 0.000 claims description 14
- 238000002525 ultrasonication Methods 0.000 claims description 13
- 108010059892 Cellulase Proteins 0.000 claims description 12
- 229940106157 cellulase Drugs 0.000 claims description 12
- 239000013049 sediment Substances 0.000 claims description 12
- 238000002137 ultrasound extraction Methods 0.000 claims description 10
- 230000015556 catabolic process Effects 0.000 claims description 8
- 238000006731 degradation reaction Methods 0.000 claims description 8
- 241000209094 Oryza Species 0.000 claims description 7
- 235000007164 Oryza sativa Nutrition 0.000 claims description 7
- 235000009566 rice Nutrition 0.000 claims description 7
- 244000131316 Panax pseudoginseng Species 0.000 claims description 6
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- 238000001914 filtration Methods 0.000 claims description 6
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- 238000000034 method Methods 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 3
- 238000000967 suction filtration Methods 0.000 claims description 3
- 238000003809 water extraction Methods 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 2
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- 238000004108 freeze drying Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 abstract description 17
- 239000003814 drug Substances 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 11
- 230000001430 anti-depressive effect Effects 0.000 abstract description 8
- 239000000935 antidepressant agent Substances 0.000 abstract description 7
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- 239000000047 product Substances 0.000 description 19
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 18
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 15
- 230000006378 damage Effects 0.000 description 11
- 229960002989 glutamic acid Drugs 0.000 description 8
- 235000013922 glutamic acid Nutrition 0.000 description 8
- 239000004220 glutamic acid Substances 0.000 description 8
- 208000027418 Wounds and injury Diseases 0.000 description 7
- 208000014674 injury Diseases 0.000 description 7
- 201000010099 disease Diseases 0.000 description 4
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- 238000012545 processing Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 206010057248 Cell death Diseases 0.000 description 2
- GIYXAJPCNFJEHY-UHFFFAOYSA-N N-methyl-3-phenyl-3-[4-(trifluoromethyl)phenoxy]-1-propanamine hydrochloride (1:1) Chemical group Cl.C=1C=CC=CC=1C(CCNC)OC1=CC=C(C(F)(F)F)C=C1 GIYXAJPCNFJEHY-UHFFFAOYSA-N 0.000 description 2
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- 229960000389 fluoxetine hydrochloride Drugs 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 208000020925 Bipolar disease Diseases 0.000 description 1
- 206010010144 Completed suicide Diseases 0.000 description 1
- 206010013710 Drug interaction Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000406668 Loxodonta cyclotis Species 0.000 description 1
- 208000019022 Mood disease Diseases 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 244000202052 Poncirus trifoliata Species 0.000 description 1
- 235000000404 Poncirus trifoliata Nutrition 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 208000028683 bipolar I disease Diseases 0.000 description 1
- 208000025307 bipolar depression Diseases 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
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- 238000002242 deionisation method Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
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- 229940049906 glutamate Drugs 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/40—Cornaceae (Dogwood family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/57—Magnoliaceae (Magnolia family)
- A61K36/575—Magnolia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8965—Asparagus, e.g. garden asparagus or asparagus fern
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K2236/30—Extraction of the material
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The present invention discloses a kind of antidepression health products containing Rice Bran Flavonoid, is made up of following raw materials by weight：Rice Bran Flavonoid 50 70%, Magnolia cortex P.E 5 10%, lucid asparagus extract 5 10%, dogwood fruit extract 5 10%, Notogineng Extract 5 10%, Bitter Orang P.E 5 10%.Initiative technology of the invention is that, as antidepressant core functionality raw material, the Chinese medicine composition for being used to treat depression of proposition, compatibility is reasonable, and being formed has antidepressant flavones, Chinese herbal medicine mixed health product using Rice Bran Flavonoid.The present invention passes through the experimental study to external depression model cell; it was found that the composition of Rice Bran Flavonoid has preferable protective effect to cell; there are better effects for depressed preventing and treating; the toxic side effect that excessive chemical synthetic drug can be avoided to cause; and it is cheap; wide material sources, also cause Rice Bran Flavonoid composition to receive significant attention.
Description
Technical field
The invention belongs to field of health care products, and in particular to a kind of antidepression health products containing Rice Bran Flavonoid.
Background technology
Depression is a kind of serious mood disorder of harm.At present, 3.5 hundred million people are there are about in the world with depression, Sweden
The incidence of disease be 6.16%, the U.S. is 4.45%, China be 3.02%.Depression seriously perplexs the live and work of patient, gives
Family and society bring white elephant, and about 15% patients with depression dies from suicide.The World Health Organization, the World Bank and Kazakhstan
One joint study of Buddhist university shows that depression has become the second serious disease of Chinese Disease Spectrum.The anti-suppression listed at present
Strongly fragrant Western medicine species and mechanism are limited, and have certain deficiency, such as there is side effect, also threaten while depression is treated
The health of other functions of patient body, or work slow, efficient not high.Clinically when going treatment depression from Western medicine
Consider treated effect, mechanism of action, from factors such as dosage, side effect, drug interactions, and for some
Complicated depression, such as Bipolar Depression, one or more diseases and depressed comorbidity, cannot preferably improve.Traditional Chinese medicine exists
Existing more than the 5000 years applicating history of China, its a large amount of experience accumulated in clinical practice is the security of its clinical practice
There is provided reliable guarantee.Efficient, low toxicity antidepressant activity composition tool is found from natural drug to have very great significance, it is existing
Help the discovery and searching of antidepression pharmacophoric group, be also beneficial to the research and development of newly-developed antidepressant.Though it will be appreciated, however, that
The research report of right Chinese medicine antidepressant effect is a lot, but research all concentrates on single component, in the research of single target spot mostly, and
The characteristics of Chinese medicine has entirety, synergy, this also implies that the action mode and action pathway between effective substance
Need further to excavate and illustrate, this is also the place of worth further investigation from now on.
The content of the invention
It is an object of the present invention to provide a kind of natural health-care products containing Rice Bran Flavonoid with antidepression function, the health products
It is that the processing byproduct rice bran of the grass family cereal crops paddy planted extensively with China sets out, prepares in Rice Bran Flavonoid and other
Medicament extract is mixed.
The technical solution adopted by the present invention is：
Antidepression health products containing Rice Bran Flavonoid, are made up of following raw materials by weight：
Rice Bran Flavonoid 50-70%, Magnolia cortex P.E 5-10%, lucid asparagus extract 5-10%,
Dogwood fruit extract 5-10%, Notogineng Extract 5-10%, Bitter Orang P.E 5-10%.
The antidepression health products containing Rice Bran Flavonoid, the preparation method of the Rice Bran Flavonoid is as follows：
1) cellulase degradation is extracted：Solid-liquid ratio in mass ratio 1:10-1:30, rice bran powder is dissolved in water, cellulase
Addition is 120-180U/g, carries out hot water extraction, and it is 50-60 DEG C to control temperature, pH value range 5.0-6.0, and enzymolysis time is
1-2h, is centrifuged, and filtering obtains that cellulase degradation extract solution is standby, sediment A proceeds ethanol assisted extraction；
2) ethanol assisted extraction：Solid-liquid ratio in mass ratio 1:10-1:30, sediment A is dissolved in ethanol, volume fraction of ethanol
For 50-70%, extraction time is 2-3h, is centrifuged, and filtering obtains that ethanol assisted extraction liquid is standby, sediment B proceeds ultrasonic leaching
Carry；
3) ultrasound extraction：Solid-liquid ratio in mass ratio 1:10-1:30, sediment B is dissolved in ethanol, volume fraction of ethanol is
50-70%, ultrasonic power is 160-200W, and ultrasonic time is 20-30min, and suction filtration obtains ultrasonic extraction extract solution standby；
4) merge cellulase degradation extract solution, ethanol assisted extraction liquid and ultrasound extraction extract solution, total extract solution is revolved
Turn to be concentrated by evaporation, after filter paper prefiltration, ultrafiltration is carried out with filter membrane, ultrafiltrate sterile cryo is dried, and obtains brown shorts flavones dry powder.
The antidepression health products containing Rice Bran Flavonoid, the preparation method of the Magnolia cortex P.E is：In room temperature condition
Under, weigh the bark of official magnolia solid powder for being ground into 200 mesh；Difference in mass ratio 1:10 ratios weigh bark of official magnolia solid powder and deionization
Water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate sterile cryo is dried, obtains the bark of official magnolia and carry
Take thing.
The antidepression health products containing Rice Bran Flavonoid, the preparation method of the lucid asparagus extract is：In room temperature bar
Under part, the lucid asparagus solid powder for being ground into 200 mesh is weighed；Difference in mass ratio 1:10 ratios weigh lucid asparagus solid powder and
Deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate sterile cryo is dried, obtained
Lucid asparagus extract.
The antidepression health products containing Rice Bran Flavonoid, the preparation method of the dogwood fruit extract is：In room temperature bar
Under part, the dogwood fruit solid powder for being ground into 200 mesh is weighed；Difference in mass ratio 1:10 ratios weigh dogwood fruit solid powder and
Deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate sterile cryo is dried, obtained
Dogwood fruit extract.
The antidepression health products containing Rice Bran Flavonoid, the preparation method of Notogineng Extract is：At ambient temperature, claim
Amount is ground into the pseudo-ginseng solid powder of 200 mesh；Difference in mass ratio 1:10 ratios weigh pseudo-ginseng solid powder and deionized water, surpass
Sound crushes 30min, and room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate sterile cryo is dried, and obtains Notogineng Extract.
The antidepression health products containing Rice Bran Flavonoid, the preparation method of Bitter Orang P.E is：At ambient temperature, claim
Amount is ground into the dried immature fruit of citron orange solid powder of 200 mesh；Difference in mass ratio 1:10 ratios weigh dried immature fruit of citron orange solid powder and deionized water, surpass
Sound crushes 30min, and room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate sterile cryo is dried, and obtains Bitter Orang P.E.
The beneficial effects of the invention are as follows：
Initiative technology of the invention is, as antidepressant core functionality raw material, to propose to be used to treat using Rice Bran Flavonoid
Depressed Chinese medicine composition, rationally, formed has antidepressant flavones, Chinese herbal medicine mixed health product to compatibility.The present invention is by right
The experimental study of external Glu-induced Injury cell, find the composition of Rice Bran Flavonoid has to external Glu-induced Injury PC12 cells
Good inhibiting effect, the effect for having highly significant for depressed preventing and treating, can avoid excessive chemical synthetic drug from making
Into toxic side effect, and cheap, wide material sources also cause Rice Bran Flavonoid composition to receive significant attention.
The present invention uses the byproduct of paddy processing --- and rice bran extracts flavones for raw material, therefrom as active material, system
Standby antidepression health products or medicine, can reach preferable extraction effect under the conditions of relatively mild, and operating method is simple, during extraction
Between it is short, low is required to apparatus for preparation, intermediate product and end-product are nontoxic, safe, therefore with broad mass market prospect, just
In batch production.
Brief description of the drawings
Fig. 1 is that various concentrations glutamic acid influences on PC12 cell strain growths.
Fig. 2 is the protective effect of various concentrations Rice Bran Flavonoid composition Glu-induced Injury PC12 cells.
Embodiment
Antidepression health products of the embodiment 1 containing Rice Bran Flavonoid
(1) preparation of Rice Bran Flavonoid
Rice Bran Flavonoid step is extracted such as using the process combination of cellulase degradation-alcohol steep-ultrasonic wave method of fractional steps
Under：
1) cellulase degradation is extracted：Rice bran is gone into the removal of impurity, dried, size-reduced machine is crushed, 60 mesh sieves is crossed, rice bran is made
Powder.Solid-liquid ratio in mass ratio 1:30, rice bran powder is dissolved in water, cellulase addition is 150U/g, carries out hot water extraction,
It is 50 DEG C to control temperature, pH 5.0, and enzymolysis time is 2h, and 4000r/min carries out centrifugation 15 minutes, and filtering obtains cellulose enzyme
Solution extract solution is standby, and sediment A proceeds alcohol steep；
2) ethanol assisted extraction：Solid-liquid ratio presses in mass ratio 1：20, sediment A is dissolved in ethanol, volume fraction of ethanol is
60%, extraction time is 3h, is centrifuged, and filtering obtains that ethanol assisted extraction liquid is standby, sediment B proceeds ultrasonic extraction；
3) ultrasound extraction：Solid-liquid ratio presses in mass ratio 1:20, sediment B is dissolved in ethanol, volume fraction of ethanol is 60%,
Ultrasonic power is 180W, and ultrasonic time is 30min, and suction filtration obtains ultrasonic extraction extract solution standby；
4) merge cellulase degradation extract solution, ethanol assisted extraction liquid and ultrasound extraction extract solution, total extract solution is revolved
Turn to be concentrated by evaporation, after filter paper prefiltration, ultrafiltration is carried out with filter membrane, ultrafiltrate sterile cryo is dried, and obtains brown shorts flavones dry powder.
(2) preparation of Magnolia cortex P.E
At ambient temperature, the bark of official magnolia solid powder for being ground into 200 mesh is weighed；In mass ratio 1:10 ratios weigh the bark of official magnolia and consolidated
Body powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate are sterile cold
It is lyophilized dry, obtain Magnolia cortex P.E.
(3) preparation of lucid asparagus extract
At ambient temperature, the lucid asparagus solid powder for being ground into 200 mesh is weighed；In mass ratio 1:10 ratios weigh Tianmen
Winter solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate without
Bacterium is freeze-dried, and obtains lucid asparagus extract.
(4) preparation of dogwood fruit extract
At ambient temperature, the dogwood fruit solid powder for being ground into 200 mesh is weighed；In mass ratio 1:10 ratios weigh mountain cornel
Meat solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate without
Bacterium is freeze-dried, and obtains dogwood fruit extract.
(5) preparation of Notogineng Extract
At ambient temperature, the pseudo-ginseng solid powder for being ground into 200 mesh is weighed；In mass ratio 1:10 ratios weigh pseudo-ginseng and consolidated
Body powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate are sterile cold
It is lyophilized dry, obtain Notogineng Extract.
(6) preparation of Bitter Orang P.E
At ambient temperature, the dried immature fruit of citron orange solid powder for being ground into 200 mesh is weighed；Difference in mass ratio 1:10 ratios weigh trifoliate orange
Real solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate without
Bacterium is freeze-dried, and obtains Bitter Orang P.E.
(7) antidepression Halth-care composition (abbreviation Rice Bran Flavonoid composition) containing Rice Bran Flavonoid
It is made up of following raw materials by weight：
Rice Bran Flavonoid 60%, Magnolia cortex P.E 5%, lucid asparagus extract 10%,
Dogwood fruit extract 5%, Notogineng Extract 10%, Bitter Orang P.E 10%
(8) MTT experiment
The present embodiment uses Glu-induced Injury cell line PC12 cells, detects that Rice Bran Flavonoid composition is external using mtt assay
Antidepressant activity, experimental procedure is as follows：
1) take the logarithm the PC12 cells in growth period, with cell number 3 × 104/ m L are inoculated in 96 well culture plates (100 μ L/
Hole), cellar culture 24h makes its adherent.
2) concentration (3.125,6.25 and 12.5mg/ml) of Rice Bran Flavonoid composition；Glutamic acid treatment group concentration (5,
10th, 15 and 20mmol/L)
3) cell is divided into Normal group, glutamic acid treatment group, positive controls and Rice Bran Flavonoid composition to pre-process
Damage group afterwards.Normal group, is routinely added to nutrient solution；Glutamate induction damage group, while adding nutrient solution and glutamic acid；Sun
Property control group, add glutamic acid and Fluoxetine hydrochloride；Medicament protection group：12h adds the pretreatment of Rice Bran Flavonoid composition in advance, it
It is synchronous with other groups again afterwards to add Glu-induced Injury.
4) MTT (5mg/ml) is added after 24h；
5) and then 96 orifice plates are placed in 37 DEG C of constant incubators and continue to cultivate 4h；
6) after processing terminates, MTT is suctioned out, 150 μ l DMSO is added, is completely dissolved first a ceremonial jade-ladle, used in libation crystal；
7) increment of PC12 cells is assessed by measuring absorbance of each hole at 490nm；As a result as shown in Figure 1, Figure 2
It is shown.
As shown in figure 1, the glutamic acid of various concentrations is incubated PC12 cell 24h respectively, MTT colorimetric determinations are then utilized.
As a result show, after handling cell for a period of time, concentration significantly makes PC12 cell deaths for 15 and 20mM/L glutamic acid.As a result table
Bright, glutamic acid can promote PC12 cell deaths in vitro, and we select 15mM/L to enter as the final concentration of Glu-induced Injury
Row experiment.
As shown in Fig. 2 the Rice Bran Flavonoid composition of various concentrations is incubated PC12 cell 12h respectively, afterwards plus glutamic acid
Damage, then utilizes MTT colorimetric determinations.Fluoxetine hydrochloride is used as positive control.As a result show, processing cell is for a period of time
Afterwards, concentration shows significantly to promote cell growth effect for 3.125,6.25 and 12.5mg/ml Rice Bran Flavonoid composition.
As a result show, Rice Bran Flavonoid composition has certain protective effect to Glu-induced Injury PC12 cells.
Antidepression health products of the embodiment 2 containing Rice Bran Flavonoid
(1)-(six) be the same as Example 1.
(7) the antidepression health products containing Rice Bran Flavonoid, are made up of following raw materials by weight：
Rice Bran Flavonoid 70%, Magnolia cortex P.E 10%, lucid asparagus extract 5%,
Dogwood fruit extract 5%, Notogineng Extract 5%, Bitter Orang P.E 5%.
Antidepression health products of the embodiment 3 containing Rice Bran Flavonoid
(1)-(six) be the same as Example 1.
(7) the antidepression health products containing Rice Bran Flavonoid, are made up of following raw materials by weight：
Rice Bran Flavonoid 50%, Magnolia cortex P.E 10%, lucid asparagus extract 10%,
Dogwood fruit extract 10%, Notogineng Extract 10%, Bitter Orang P.E 10%.
Claims (7)
1. the antidepression health products containing Rice Bran Flavonoid, it is characterised in that be made up of following raw materials by weight：
Rice Bran Flavonoid 50-70%, Magnolia cortex P.E 5-10%, lucid asparagus extract 5-10%,
Dogwood fruit extract 5-10%, Notogineng Extract 5-10%, Bitter Orang P.E 5-10%.
2. the antidepression health products as claimed in claim 1 containing Rice Bran Flavonoid, it is characterised in that the preparation of the Rice Bran Flavonoid
Method is as follows：
1) cellulase degradation is extracted：Solid-liquid ratio in mass ratio 1:10-1:30, rice bran powder is dissolved in water, cellulase is added
Measure as 120-180U/g, carry out hot water extraction, it is 50-60 DEG C control temperature, pH value range 5.0-6.0, and enzymolysis time is 1-2h,
Centrifugation, filtering, obtains that cellulase degradation extract solution is standby, and sediment A proceeds ethanol assisted extraction；
2) ethanol assisted extraction：Solid-liquid ratio in mass ratio 1:10-1:30, sediment A is dissolved in ethanol, volume fraction of ethanol is
50-70%, extraction time is 2-3h, is centrifuged, and filtering obtains that ethanol assisted extraction liquid is standby, sediment B proceeds ultrasonic leaching
Carry；
3) ultrasound extraction：Solid-liquid ratio in mass ratio 1:10-1:30, sediment B is dissolved in ethanol, volume fraction of ethanol is 50-
70%, ultrasonic power is 160-200W, and ultrasonic time is 20-30min, and suction filtration obtains ultrasonic extraction extract solution standby；
4) merge cellulase degradation extract solution, ethanol assisted extraction liquid and ultrasound extraction extract solution, total extract solution is rotated and steamed
After hair concentration, filter paper prefiltration, ultrafiltration is carried out with filter membrane, ultrafiltrate sterile cryo is dried, and obtains brown shorts flavones dry powder.
3. the antidepression health products as claimed in claim 1 containing Rice Bran Flavonoid, it is characterised in that the system of the Magnolia cortex P.E
Preparation Method is：At ambient temperature, the bark of official magnolia solid powder for being ground into 200 mesh is weighed；Difference in mass ratio 1:10 ratios are weighed
Bark of official magnolia solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate
Sterile cryo is dried, and obtains Magnolia cortex P.E.
4. the antidepression health products as claimed in claim 1 containing Rice Bran Flavonoid, it is characterised in that the lucid asparagus extract
Preparation method is：At ambient temperature, the lucid asparagus solid powder for being ground into 200 mesh is weighed；Difference in mass ratio 1:10 ratios
Weigh lucid asparagus solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration,
Ultrafiltrate sterile cryo is dried, and obtains lucid asparagus extract.
5. the antidepression health products as claimed in claim 1 containing Rice Bran Flavonoid, it is characterised in that the dogwood fruit extract
Preparation method is：At ambient temperature, the dogwood fruit solid powder for being ground into 200 mesh is weighed；Difference in mass ratio 1:10 ratios
Weigh dogwood fruit solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration,
Ultrafiltrate sterile cryo is dried, and obtains dogwood fruit extract.
6. the antidepression health products as claimed in claim 1 containing Rice Bran Flavonoid, it is characterised in that the system of the Notogineng Extract
Preparation Method is：At ambient temperature, the pseudo-ginseng solid powder for being ground into 200 mesh is weighed；Difference in mass ratio 1:10 ratios are weighed
Pseudo-ginseng solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate
Sterile cryo is dried, and obtains Notogineng Extract.
7. the antidepression health products as claimed in claim 1 containing Rice Bran Flavonoid, it is characterised in that the preparation side of Bitter Orang P.E
Method is：At ambient temperature, the dried immature fruit of citron orange solid powder for being ground into 200 mesh is weighed；Difference in mass ratio 1:10 ratios weigh the dried immature fruit of citron orange
Solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate are sterile
Freeze-drying, obtains Bitter Orang P.E.
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| CN201710362756.4A CN106983830A (en) | 2017-05-22 | 2017-05-22 | Antidepression health products containing Rice Bran Flavonoid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109907319A (en) * | 2019-03-12 | 2019-06-21 | 中南林业科技大学 | A kind of rice bran extract and preparation method thereof and the application in health food |
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| CN103561755A (en) * | 2011-05-27 | 2014-02-05 | 韩国食品研究院 | Novel usage of rice, rice bran, or chaff extract as histamine receptor antagonist |
| CN105399717A (en) * | 2015-12-16 | 2016-03-16 | 吉林市莹洁科技开发有限公司 | Method for extracting flavones from rice bran |
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2017
- 2017-05-22 CN CN201710362756.4A patent/CN106983830A/en active Pending
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|---|---|---|---|---|
| CN103561755A (en) * | 2011-05-27 | 2014-02-05 | 韩国食品研究院 | Novel usage of rice, rice bran, or chaff extract as histamine receptor antagonist |
| CN105399717A (en) * | 2015-12-16 | 2016-03-16 | 吉林市莹洁科技开发有限公司 | Method for extracting flavones from rice bran |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109907319A (en) * | 2019-03-12 | 2019-06-21 | 中南林业科技大学 | A kind of rice bran extract and preparation method thereof and the application in health food |
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