CN107167621A - Magnetic microparticle chemiluminescence immune assay instrument multi-channel parallel method - Google Patents
Magnetic microparticle chemiluminescence immune assay instrument multi-channel parallel method Download PDFInfo
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- CN107167621A CN107167621A CN201610127319.XA CN201610127319A CN107167621A CN 107167621 A CN107167621 A CN 107167621A CN 201610127319 A CN201610127319 A CN 201610127319A CN 107167621 A CN107167621 A CN 107167621A
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- Prior art keywords
- cup
- transported
- bar
- reagent
- detection
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
Abstract
It is detachably loaded with respect to the invention discloses a kind of magnetic microparticle chemiluminescence immune assay instrument multi-channel parallel method, including by reaction cup in glass bar, sample is detected to sample channel filling is added；Cup bar is transported to reagent adding passage, and detection reagent is filled into reaction cup and reagent mixing passage is transported to；Incubation system is transported to be incubated the detection sample in reaction cup；Washing channel is transported to be washed；Cup bar is transported to substrate filling and passage filling substrate is mixed；Being transported to detection waits passage etc. to be detected；Reaction cup in passage is waited to capture into detecting system detection one by one detection；And be transported to empty cup bar plus the step such as sample channel；Wherein, plus sample channel, reagent adding passage and washing channel be at least two.The present invention substantially increases detection efficiency while detection flexibility is ensured, and cup bar can circulating repetition utilize, consumptive material consumption is few, and testing cost is relatively low.
Description
Technical field
The present invention relates to a kind of magnetic microparticle chemiluminescence immune assay instrument multi-channel parallel method, belong to detection point
Analyse technical field.
Background technology
Magnetic particle luminescence immunoassay is one kind that chemiluminescence immunoassay method is combined with magnetic particle isolation technics
Detection method, due to magnetic particle have low magnetic responsiveness, cost, less energy consumption and it is pollution-free the features such as, can
Functional group (such as amino, hydroxyl, sulfydryl and oxirane) on magnetic particle surface or magnetic particle surface
The bioactive substances such as enzyme, antibody, oligonucleotides are fixed, traditional immune detection is more with ELISA Plate
As solid phase carrier, suspension magnetic particle has higher specific area as carrier, can more sufficiently and sample
Product react, the flexible Application of externally-applied magnetic field in addition, have higher sensitivity, faster than ELISA Plate carrier
Detection speed and preferably repeatability the advantages of, therefore magnetic particle luminescence immunoassay instrument immunoassay lead
Domain has its original advantage.
Magnetic particle luminescence immunoassay instrument transports analysis sample when being detected, it is necessary to be loaded using reaction cup,
And experience filling reagent, incubation, washing plus substrate and blending process, tradition are also needed to before testing
Magnetic particle luminescence immunoassay instrument generally uses disc type single channel circulation conveying structure, is usually by multiple reactions
Cup is fixed as one group and integrally conveyed or single reaction cup is conveyed one by one, and when being conveyed using integral way, its is flexible
Property it is poor, it is difficult to the need for adapting to the diversified sample presentation detection of small lot, when one cup is conveyed one by one, it conveys effect
Rate is relatively low, seriously limits the detection efficiency of magnetic particle luminescence immunoassay instrument.
In addition, number of times of the magnetic particle luminescence immunoassay according to filling reagent, its analysis process is also divided into a step
Method and two-step method, during using two-step method, after first time filling reagent is incubated, in addition it is also necessary to add reagent again
Subsequent analysis process is then carried out again, and when part, detection sample needs to be detected with one-step method, partly needs to use two
When footwork is detected, traditional magnetic particle luminescence immunoassay instrument can only be carried out one by one, it is difficult to met it and detected needs.
The content of the invention
To solve above technical problem, the present invention provides a kind of magnetic microparticle chemiluminescence immune assay instrument multichannel
Parallel method, to improve the detection efficiency of magnetic microparticle chemiluminescence immune assay instrument, and ensures its flexibility.
To achieve the above object, technical solution of the present invention is as follows：
A kind of magnetic microparticle chemiluminescence immune assay instrument multi-channel parallel method, comprises the following steps：
Reaction cup is sorted and is detachably loaded with respect in glass bar, is put into and adds sample channel to fill detection sample；
The cup bar after detection sample will be filled and be transported to reagent adding passage, and by reagent loading system to reaction
Detection reagent is filled in cup；
The cup bar after detection reagent will be filled, mixing passage by reagent carries out reagent mixing；
Cup bar after mixing is transported into incubation system to be incubated the detection sample in reaction cup；
Detect that corresponding cup bar after incubation, is transported to washing channel, reaction cup is washed by sample；
After reaction cup washing, corresponding cup bar is transported to substrate filling and mixing passage filling substrate and mixed；
The corresponding cup bar filled after substrate and mixing is transported into detection waits passage etc. to be detected；
It will detect that wait the reaction cup in passage on cup bar to be captured one by one by handgrip is examined into detecting system
Survey；
And wait the empty cup bar for having removed reaction cup in passage to be transported to plus sample channel by detecting；
Wherein, described plus sample channel, reagent adding passage and washing channel are at least two.
Using above-mentioned technical proposal, multiple reaction cups are constituted one to transport, detecting system is utilized when detecting
Handgrip is captured one by one, that is, ensure that flexibility, while detection efficiency is substantially increased, due to using multichannel
Parallel mode, part detection sample does not influence the efficiency that other one-step method are detected when being detected using two-step method, separately
Outside, cup bar can circulating repetition utilize, reduce consumptive material consumption, saved testing cost.
To ensure to transport efficiency, the transport of the cup bar send glass dolly to carry out, respectively send cup small by least two
The action of car is controlled by control system, and control system can monitor the working condition for sending glass dolly, it is necessary to transport
When sending glass bar, what control was currently at idle condition send a glass dolly to be transported.
Preferably, reaction cup upper end both sides are provided with crawl neck, to facilitate handgrip to capture, cup bar is length
Strip structure, is distributed with the reaction cup neck of at least one up/down perforation along its length, the reaction cup neck
Shape is adapted with reaction cup outer rim shape.
The cup bar two ends are positioned and transported for convenience provided with location division, the location division upper and lower ends are protruded instead
Cup neck end face is answered, the location division is provided with the locating groove of arc, institute provided with transfer hole, location division both sides
State plus sample channel, reagent adding passage and washing channel are groove-like structure corresponding with cup strip, work as cup
When bar is located on each passage, it is possible to use locating groove is positioned, during transport, is sent and is accordingly tied on glass dolly
Structure stretches into transfer hole to facilitate dragging to be transported.
Further to improve detection efficiency, the reagent loading system includes at least one reagent and fills pin, can be with
It is simultaneously multiple reaction cups filling reagent on cup bar.
Beneficial effect：
Multiple separate reaction cups are placed on same cup bar by the present invention is transported, before final detection
Each step uses multi-channel parallel mode, and detecting system is captured and detected one by one using handgrip, is ensureing
While detecting flexibility, detection efficiency is substantially increased, and cup bar being capable of circulating repetition utilization, consumptive material
Consumption is few, and testing cost is relatively low.
Brief description of the drawings
Fig. 1 is flow chart of the invention；
Fig. 2 is the structure principle chart of magnetic microparticle chemiluminescence immune assay instrument in the present invention；
Fig. 3 is the structural representation of reaction cup；
Fig. 4 is the structural representation of cup bar；
Fig. 5 is the structural representation that reaction cup loads after cup bar；
Fig. 6 is structural representation when cup bar is located at each passage.
Embodiment
The invention will be further described with accompanying drawing with reference to embodiments.
As shown in Figures 1 to 6, a kind of magnetic microparticle chemiluminescence immune assay instrument multi-channel parallel method, including
Following steps：
S1：By multiple reaction cups 1 after the sequence of row's cup system, cup is detachably loaded with respect to by sending cup to move cup handgrip
In bar 2, it is put into and adds in sample channel A by filling filling pin or TP filling detection samples in sample system；
S2：The cup bar 2 after detection sample will be filled and send a glass dolly 4a to be transported to reagent adding passage B by first,
And detection reagent is filled into reaction cup 1 by reagent loading system；
S3：The cup bar 2 after detection reagent will be filled send a glass dolly 4a to be transported to reagent mixing channel C by first,
Mix the detection sample and reagent in reaction cup 1；
S4：Glass dolly 4b is sent to be transported to incubation system D to the detection sample in reaction cup 1 by second after mixing
It is incubated；
S41：In the case of the special reagent of part, after detection sample incubation, corresponding cup bar 2 is transported to reagent adding
Passage B, repeats S2~S4 steps；
S5：Sample is detected after incubation, second send glass dolly 4b that corresponding cup bar 2 is transported into washing channel E,
Reaction cup 1 is washed, to clean the impurity after incubation in reaction cup 1；
S51：When using two-step method, after reaction cup 1 is washed, glass dolly 4a is sent by corresponding cup bar 2 by first
Reagent adding passage B is transported to, S2~S5 steps are repeated；
S6：After reaction cup 1 is washed, glass dolly 4a or second is sent to send glass dolly 4b by corresponding cup bar 2 by first
It is transported to substrate filling and mixes passage F filling substrates and mix；
S7：Fill after substrate, send glass dolly 4a or second to send glass dolly 4b to transport corresponding cup bar 2 by first
Delivering to detection waits passage G etc. to be detected；
S8：To detect wait reaction cup 1 in passage G on cup bar 2 by detect move cup handgrip 3 capture one by one to
Detected in detecting system H, the reaction cup 1 after the completion of detection is moved cup handgrip 3 using the detection and lost into trash repository
In；
S9：To finally detect waits the empty cup bar 2 for having removed reaction cup 1 in passage F to send a glass dolly by first
4a is transported to plus sample channel A, waits next testing process.
The transport of cup bar 2 send glass dolly 4 to complete by least two, send the structure of glass dolly 4 in Chinese invention
Disclosure is able in the documents such as patent application CN104646343A, CN104535760A, therefore not to repeat here, this
In embodiment, preferably two, glass dolly 4 is sent, including first send glass dolly 4a and second to send glass a dolly 4b, phase
Mutually it be arranged in parallel, above-mentioned steps S2, S3, S51, S9 send a glass dolly 4a individually to complete by first, step S4,
S5 individually send glass dolly 4b to complete by second, and step S6 and S7 are then sent by the control system detection two of moving cart 4
Wherein one or two in cup dolly 4 in idle condition is carried out simultaneously, and step S41 first send cup small by second
Car 4b is transported to reagent and mixes channel C, then send a glass dolly 4a to be transported to reagent adding passage B by first.
Fig. 3 and Fig. 4 illustrate the concrete structure of reaction cup 1 and cup bar 2 respectively, it can be seen that reaction cup 1 is big
The cup-shape in upper end open lower end closed is caused, its upper end end is circumferentially outwardly.
The overall elongated structure of cup bar 2, two ends are provided with location division 22, and middle part is distributed with multiple along its length
Reaction cup neck 21 is preferably in shape reaction cup neck 21 corresponding with the outer rim shape of reaction cup 1, the present embodiment
6.
Reaction cup neck 21 is the through-hole structure of up/down perforation, and the height of two ends location division 22 is reacted more than middle part
The height of cup neck 21 part, and the upper and lower ends of location division 22 protrude the end face of reaction cup neck 21, i.e., in the middle part of
Reaction cup neck 21 be partially in overhead positions.
The transfer hole 22a of blind hole structure is vertically provided with two location divisions 22, a glass dolly is sent to facilitate
4 transport cup bar, and the both sides of location division 22 are provided with the locating groove 22b of arc, when cup bar 2 is in above-mentioned passage,
Cup bar 2 can be carried out using locating groove 22b spacing.
With reference to Fig. 5 as can be seen that when reaction cup 1 loads cup bar 2, the upper end projection of reaction cup 1 is with reacting
Cup neck 21 upper surface is abutted, and the lower surface of reaction cup neck 21 is stretched out in the lower end of cup bar 2, and convenient vibration is mixed.
Fig. 6 is the structural representation that the cup bar 2 equipped with reaction cup 1 is in respective channel, is described for convenience, this
Embodiment is by taking reagent adding passage B as an example, and rest channels structure is approximate with its, is shape and the profile phase of cup bar 1
The groove-like structure answered, but fluting quantity difference as needed, in reagent adding passage B outer rims along its length
Direction is provided with shell fragment 6, and the end of shell fragment 6 is fixed with limited block 6a, at least a portion knot on limited block 6a
Structure is corresponding to the locating groove 22b on cup bar 2, when cup bar 2 is placed on reagent adding passage B, limited block
6a in embedded locating groove 22b, is carried out spacing under the effect of shell fragment 6 to cup bar 2.
It is preferably 3 to improve the reagent filling pin 5 in reagent filling efficiency, the present embodiment in reagent loading system
Individual, 6 reaction cups on correspondence cup bar 2 in 6 reaction cup necks 21 it also avoid while guaranteed efficiency
System load is excessive.
Finally it is to be appreciated that foregoing description is only the preferred embodiments of the present invention, those skilled in the art exists
Under the enlightenment of the present invention, on the premise of without prejudice to present inventive concept and claim, multiple types can be made
As represent, such conversion each fallen within protection scope of the present invention.
Claims (5)
1. a kind of magnetic microparticle chemiluminescence immune assay instrument multi-channel parallel method, it is characterised in that including with
Lower step：
By reaction cup (1) sort and be detachably loaded with respect in a glass bar (2), be put into plus sample channel (A) with
Filling detection sample；
The cup bar (2) filled after detection sample is transported to reagent adding passage (B), and system is filled by reagent
Unite and detection reagent is filled into reaction cup (1)；
The cup bar (2) after detection reagent will be filled, mixing passage (C) by reagent carries out reagent mixing；
Cup bar (2) after mixing is transported into incubation system (D) to enter the detection sample in reaction cup (1)
Row is incubated；
Detect that corresponding cup bar (2) after incubation, is transported to washing channel (E), to reaction cup (1) by sample
Washed；
After reaction cup (1) washing, corresponding cup bar (2) is transported to substrate filling and mixing passage (F) adds
Note substrate is simultaneously mixed；
The corresponding cup bar (2) filled after substrate is transported into detection waits passage (G) etc. to be detected；
The reaction cup (1) on passage (G) interior cup bar (2) is waited to be grabbed one by one by handgrip (3) by detecting
Take into detecting system (H) and detected；
And will detect that waiting the empty cup bar (2) for having removed reaction cup (1) in passage (F) to be transported to adds
Sample channel (A)；
Wherein, described plus sample channel (A), reagent adding passage (B) and washing channel (E) are at least
Two.
2. magnetic microparticle chemiluminescence immune assay instrument multi-channel parallel method according to claim 1, its
It is characterised by：The transport of the cup bar (2) send a glass dolly (4) to carry out by least two.
3. magnetic microparticle chemiluminescence immune assay instrument multi-channel parallel method according to claim 1, its
It is characterised by：The cup bar (2) is string configuration, and at least one up/down perforation is distributed with along its length
Reaction cup neck (21), shape and reaction cup (1) outer rim shape of the reaction cup neck (21) are mutually fitted
Match somebody with somebody.
4. magnetic microparticle chemiluminescence immune assay instrument multi-channel parallel method according to claim 3, its
It is characterised by：Cup bar (2) two ends are provided with location division (22), and location division (22) upper and lower ends are equal
Reaction cup neck (21) end face is protruded, the location division (22) is provided with transfer hole (22a), location division (22)
Both sides are provided with the locating groove (22b) of arc.
5. magnetic microparticle chemiluminescence immune assay instrument multichannel according to any one of claim 1 to 4 is simultaneously
Row method, it is characterised in that：The reagent loading system includes at least one reagent filling pin (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610127319.XA CN107167621B (en) | 2016-03-07 | 2016-03-07 | Magnetic microparticle chemiluminescence immune assay instrument multicenter detecting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610127319.XA CN107167621B (en) | 2016-03-07 | 2016-03-07 | Magnetic microparticle chemiluminescence immune assay instrument multicenter detecting method |
Publications (2)
| Publication Number | Publication Date |
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| CN107167621A true CN107167621A (en) | 2017-09-15 |
| CN107167621B CN107167621B (en) | 2019-11-05 |
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| CN201610127319.XA Active CN107167621B (en) | 2016-03-07 | 2016-03-07 | Magnetic microparticle chemiluminescence immune assay instrument multicenter detecting method |
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Cited By (7)
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| CN107664635A (en) * | 2017-11-17 | 2018-02-06 | 南通伊仕生物技术股份有限公司 | A kind of chemiluminescence detection reagent strip |
| CN107831325A (en) * | 2017-10-20 | 2018-03-23 | 厦门市波生生物技术有限公司 | Full-automatic chemiluminescence immunoassay analysis meter |
| CN108226549A (en) * | 2018-01-15 | 2018-06-29 | 重庆博奥新景医学科技有限公司 | A kind of sequential control method and system for chemical illumination immunity analysis instrument |
| CN108614101A (en) * | 2018-03-14 | 2018-10-02 | 嘉兴科瑞迪医疗器械有限公司 | A kind of magnetic particle Full-automatic chemiluminescence immunoassay analysis meter |
| CN110082291A (en) * | 2018-01-25 | 2019-08-02 | 深圳市新产业生物医学工程股份有限公司 | Adsorbing mechanism, cleaning device, chemiluminescence detector and cleaning method |
| CN110346557A (en) * | 2019-07-15 | 2019-10-18 | 深圳海思安生物技术有限公司 | A kind of detection kit |
| CN111413264A (en) * | 2020-04-02 | 2020-07-14 | 华中农业大学 | Multi-channel particle detection device and method for detecting micron particles |
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| CN107831325A (en) * | 2017-10-20 | 2018-03-23 | 厦门市波生生物技术有限公司 | Full-automatic chemiluminescence immunoassay analysis meter |
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| CN111413264B (en) * | 2020-04-02 | 2021-04-06 | 华中农业大学 | Multi-channel particle detection device and method for detecting micron particles |
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