CN107664635A - A kind of chemiluminescence detection reagent strip - Google Patents
A kind of chemiluminescence detection reagent strip Download PDFInfo
- Publication number
- CN107664635A CN107664635A CN201711147040.9A CN201711147040A CN107664635A CN 107664635 A CN107664635 A CN 107664635A CN 201711147040 A CN201711147040 A CN 201711147040A CN 107664635 A CN107664635 A CN 107664635A
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- CN
- China
- Prior art keywords
- hole
- reagent
- reagent strip
- capacity
- washing lotion
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
Abstract
The present invention discloses a kind of chemiluminescence detection reagent strip, belongs to chemiluminescence immunoassay technology field.Chemiluminescence detection reagent strip both ends of the present invention, which are respectively equipped with, surveys pin sensing and sample aperture, is close to sample aperture and is sequentially provided with tip head bore and magnetosheath hole, reagent wells, substrate hole and clean-out opening are additionally provided with reagent strip.Chemiluminescence detection reagent strip of the present invention is easy to use, easy to spread.
Description
Technical field
The present invention relates to chemiluminescence immunoassay technology field, and in particular to a kind of chemiluminescence detection reagent strip.
Background technology
In POCT detection techniques, the detection method of small-sized POCT projects is nearly all immunochromatographic method in the market,
Either fluorescent quantitation or collaurum, all it is immunochromatographic method for essence, is all test strips.Immunochromatographic method can not be real
Now complete quantitative, detection sensitivity is relatively low, and main flow instrument is different platform, and testing result can not across comparison.Therefore market needs
The small-sized chemical luminescence detector separated using particulate is wanted, the instrument is entirely quantitative, and detection sensitivity is high, and main flow instrument
The same platform of device, it is possible to achieve across comparison.
So in detection speed, the main flow of domestic immune instrument test speed is 200 fast in the market, market competition
It is fierce.And promoted from market development and country in terms of the trend of classification diagnosis and treatment, the immune user of this large-scale immune instrument test
Object will certainly sink, and following vast primary hospitals, community clinic and community hospital will all carry out.But with the progress of science and technology
And development, it is that every usage amount is smaller the characteristics of this portions of client object, user of service's quality is relatively low, therefore market is with greater need for can be single
The instrument of item detection, and the chemiluminescence detection reagent strip of simultaneously supporting single item detection instrument is even more trend of the situation.
The content of the invention
Therefore, the technical problem to be solved in the present invention be to overcome lack in the prior art it is supporting can single item detection instrument
Chemiluminescence detection reagent strip the defects of, so as to provide a kind of chemiluminescence detection reagent strip.
Therefore, the present invention provides following technical scheme：
A kind of chemiluminescence detection reagent strip, the both ends of the reagent strip, which are respectively equipped with, surveys pin sensing and sample aperture, is close to sample
This hole is sequentially provided with tip head bore and magnetosheath hole, and reagent wells, substrate hole and clean-out opening are additionally provided with reagent strip.
It is additionally provided with dilution fluid apertures and washing lotion hole.
The magnetic bead for being coated with biological reagent for test is placed with the reagent wells, being loaded with washing lotion and dilution
Washing lotion hole and dilution fluid apertures, substrate hole and reagent wells are by sealer.
The reagent wells are 1-3, for holding reagent.
Also include incubating hole, for constant energy reaction temperature, range temperature is constantly maintained in the range of needs.
The clean-out opening is 1-4.
The washing lotion hole, substrate hole and reagent wells and dilution fluid apertures are close to magnetosheath hole and continuously set.
The cleaning fluid apertures and incubation hole are close to survey pin sensing and continuously set.
The capacity for surveying pin sensing is 100-27 μ l, and the capacity of the cleaning fluid apertures is 200-800 μ l, the incubation hole
Capacity is 800 μ l, and the capacity in the washing lotion hole is 1.2ml, and the capacity of the reagent wells is 190 μ l, the capacity in the substrate hole
For 100-370 μ l, the capacity of the dilution fluid apertures is 5.9ml, and the capacity of the sample aperture is 30-190 μ l.
The present invention also provides the kit for including the reagent strip.
Technical solution of the present invention, have the following advantages that：
1. the both ends of chemiluminescence detection reagent strip provided by the invention, which are respectively equipped with, surveys pin sensing and sample aperture, it is close to sample
Hole is sequentially provided with tip head bore and magnetosheath hole, and reagent wells, substrate hole and clean-out opening are additionally provided with reagent strip, during use,
Reagent strip is also placed with magnetosheath and tip heads in addition to equipped with reagent, as long as the sample of required test is put into sample aperture, just directly
It can be detected by detection probe, finish the magnetosheath discarded after test and tip heads are all put back in reagent strip, it is only necessary to reagent strip
Take out, it is possible to do next group of experiment again.Avoid and periodic cleaning and cleaning are carried out to discarded object and liquid.
2. the survey pin sensing material requirements of chemiluminescence detection reagent strip provided by the invention has shade function, individually as one
Individual part is positioned in reagent strip.The magnetosheath and tip heads discarded after experiment all put back to corresponding recovery hole position, it is only necessary to take out examination
Agent bar, with regard to next group of experiment can be done.
3. the magnetic bead for being coated with biological reagent is placed with the actual apertures of chemiluminescence detection reagent strip provided by the invention, can
To be adsorbed using bar magnet or other magnetic objects and then in corresponding reagent and substrate reactions, reuse probe inspection
Survey.
Brief description of the drawings
, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical scheme of the prior art
The required accompanying drawing used is briefly described in embodiment or description of the prior art, it should be apparent that, in describing below
Accompanying drawing is some embodiments of the present invention, for those of ordinary skill in the art, before creative work is not paid
Put, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is chemiluminescence detection reagent strip top view of the present invention；
Fig. 2 is chemiluminescence detection reagent strip upward view of the present invention；
Description of reference numerals：
1- surveys pin sensing；2- sample apertures；3-tip head bore 3；4- magnetosheaths hole；5- reagent wells；6- substrates hole；7- clean-out openings；8- is dilute
Release fluid apertures；9- washing lotions hole；10- incubates hole.
Embodiment
Technical scheme is clearly and completely described below in conjunction with accompanying drawing, it is clear that described implementation
Example is part of the embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, ordinary skill
The every other embodiment that personnel are obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
As long as in addition, technical characteristic involved in invention described below different embodiments non-structure each other
It is be combined with each other into conflict can.
The principle explanation of reagent strip of the present invention：
Using liquid-transfering device according to the dilution being actually needed in absorption reagent strip and the magnetic bead for being coated with biological reagent
Or other reagents and adsorption reaction is completed, magnetic bead then is adsorbed using magnetic devices, cleans the magnetic bead fully reacted afterwards, then with
Substrate prepares corresponding detection solution, detects fluorescent value using detection probe so as to complete test detection.
Embodiment 1
The present embodiment provides a kind of chemiluminescence detection reagent strip, and the both ends of the reagent strip, which are respectively equipped with, surveys the He of pin sensing 1
Sample aperture 2, it is close to sample aperture 2 and is sequentially provided with tip head bore 3 and magnetosheath hole 4, reagent wells 5, the and of substrate hole 6 is additionally provided with reagent strip
Clean-out opening 7.
It is additionally provided with dilution fluid apertures 8 and washing lotion hole 9.
The magnetic bead for being coated with biological reagent for test is placed with the reagent wells 5, being loaded with washing lotion and dilution
Washing lotion hole 9 and dilution fluid apertures 8, substrate hole 6 and reagent wells 5 by sealer.
The reagent wells 5 are 1-3, for holding reagent.
Also include incubating hole 10, for for constant reaction temperature.
The clean-out opening 7 is 1-4.
The clean-out opening 7, substrate hole 6 and reagent wells 5 and dilution fluid apertures 8 are close to magnetosheath hole 4 and continuously set.
The clean-out opening 7 and incubation hole 10 are close to survey pin sensing 1 and continuously set.
The capacity for surveying pin sensing 1 is 100-27 μ l, and the capacity in the washing lotion hole 9 is 200-800 μ l, the incubation hole 10
Capacity be 800 μ l, the capacity in the washing lotion hole is 1.2ml, and the capacity of the reagent wells 5 is 190 μ l, the substrate hole 6
Capacity is 100-370 μ l, and the capacity of the dilution fluid apertures 8 is 5.9ml, and the capacity of the sample aperture is 30-190 μ l.
The present invention also provides the kit for including the reagent strip.
Obviously, above-described embodiment is only intended to clearly illustrate example, and is not the restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or
Among changing still in the protection domain of the invention.
Claims (10)
1. a kind of chemiluminescence detection reagent strip, it is characterised in that the both ends of the reagent strip, which are respectively equipped with, surveys pin sensing and sample
Hole, it is close to sample aperture and is sequentially provided with tip head bore and magnetosheath hole, reagent wells, substrate hole and clean-out opening are additionally provided with reagent strip.
2. reagent strip according to claim 1, it is characterised in that be additionally provided with dilution fluid apertures and washing lotion hole.
3. reagent strip according to claim 2, it is characterised in that the magnetic bead for being coated with biological reagent for test is placed
In the reagent wells, it is loaded with the washing lotion hole of washing lotion and dilution and dilutes fluid apertures, substrate hole and reagent wells by sealer.
4. reagent strip according to claim 3, it is characterised in that the reagent wells are 1-3, for holding reagent.
5. reagent strip according to claim 4, it is characterised in that also include incubating hole, for constant reaction temperature.
6. the reagent strip stated according to claim 5, it is characterised in that the clean-out opening is 1-4.
7. reagent strip according to claim 6, it is characterised in that the washing lotion hole, substrate hole and reagent wells and dilution
Fluid apertures is close to magnetosheath hole and continuously set.
8. reagent strip according to claim 7, it is characterised in that it is continuous that survey pin sensing is close in the cleaning fluid apertures and incubation hole
Set.
9. reagent strip according to claim 7, it is characterised in that the capacity for surveying pin sensing is 100-27 μ l, described clear
The capacity in washing lotion hole is 200-800 μ l, and the capacity for incubating hole is 800 μ l, and the capacity in the washing lotion hole is 1.2ml, described
The capacity of reagent wells is 190 μ l, and the capacity in the substrate hole is 100-370 μ l, and the capacity of the dilution fluid apertures is 5.9ml, institute
The capacity for stating sample aperture is 30-190 μ l.
10. include the kit of any reagent strips of claim 1-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711147040.9A CN107664635A (en) | 2017-11-17 | 2017-11-17 | A kind of chemiluminescence detection reagent strip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711147040.9A CN107664635A (en) | 2017-11-17 | 2017-11-17 | A kind of chemiluminescence detection reagent strip |
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| Publication Number | Publication Date |
|---|---|
| CN107664635A true CN107664635A (en) | 2018-02-06 |
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ID=61144386
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711147040.9A Pending CN107664635A (en) | 2017-11-17 | 2017-11-17 | A kind of chemiluminescence detection reagent strip |
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| CN (1) | CN107664635A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109018612A (en) * | 2018-07-05 | 2018-12-18 | 嘉兴凯实生物科技有限公司 | A kind of reagent strip and kit |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103604939A (en) * | 2013-12-03 | 2014-02-26 | 南京医科大学第二附属医院 | Full-automatic luminescent immunoassay system based on micronano-magnetic bead electromagnetic transfer technique |
| CN203881782U (en) * | 2014-04-01 | 2014-10-15 | 重庆科斯迈生物科技有限公司 | Cup position detection system of chemiluminiscence immunoassay analyzer |
| CN206339561U (en) * | 2016-07-05 | 2017-07-18 | 深圳普门科技有限公司 | Small electrical chemical illumination immunity analysis instrument |
| CN107167621A (en) * | 2016-03-07 | 2017-09-15 | 重庆科斯迈生物科技有限公司 | Magnetic microparticle chemiluminescence immune assay instrument multi-channel parallel method |
| CN206583802U (en) * | 2017-02-14 | 2017-10-24 | 北京源诚科仪生物技术有限公司 | Miniature full-automatic chemical luminescence detector |
-
2017
- 2017-11-17 CN CN201711147040.9A patent/CN107664635A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103604939A (en) * | 2013-12-03 | 2014-02-26 | 南京医科大学第二附属医院 | Full-automatic luminescent immunoassay system based on micronano-magnetic bead electromagnetic transfer technique |
| CN203881782U (en) * | 2014-04-01 | 2014-10-15 | 重庆科斯迈生物科技有限公司 | Cup position detection system of chemiluminiscence immunoassay analyzer |
| CN107167621A (en) * | 2016-03-07 | 2017-09-15 | 重庆科斯迈生物科技有限公司 | Magnetic microparticle chemiluminescence immune assay instrument multi-channel parallel method |
| CN206339561U (en) * | 2016-07-05 | 2017-07-18 | 深圳普门科技有限公司 | Small electrical chemical illumination immunity analysis instrument |
| CN206583802U (en) * | 2017-02-14 | 2017-10-24 | 北京源诚科仪生物技术有限公司 | Miniature full-automatic chemical luminescence detector |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109018612A (en) * | 2018-07-05 | 2018-12-18 | 嘉兴凯实生物科技有限公司 | A kind of reagent strip and kit |
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Application publication date: 20180206 |