CN107125026A - A kind of cultural method of cordyceps sinensis - Google Patents
A kind of cultural method of cordyceps sinensis Download PDFInfo
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- CN107125026A CN107125026A CN201710366920.9A CN201710366920A CN107125026A CN 107125026 A CN107125026 A CN 107125026A CN 201710366920 A CN201710366920 A CN 201710366920A CN 107125026 A CN107125026 A CN 107125026A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention discloses a kind of cultural method of cordyceps sinensis, it comprises the steps：（1）Prepare spore suspension：By the parts by weight of mealy potato 220, the parts by weight of white sugar 21, the parts by weight of potassium dihydrogen phosphate 3, the parts by weight of magnesium sulfate 1.5, vitamin B1210 parts by weight, the parts by weight of production increasing agent 0.5, which are well mixed after high-temperature sterilization, obtains fluid nutrient medium, and cordyceps sinensis spore is placed in the fluid nutrient medium, is fermented 10 days in 20 23 DEG C of lucifuges, spore suspension is made；（2）The selection in place：Set up multilayer yoke, every layer spread 7 cm thicks sandy soil, sand moisture kept on 40%, soil with leaf or debris covering；（3）Apply liquid cultivation：Choosing 2 makes the bat moth larvae of larval stage for host insect, dilutes spore suspension, is then uniformly applied on each host insect；By the host insect seed covering in step（2）Cultivated 3 months in described sandy soil., can be with large scale cultivating cordyceps sinensis according to the cultural method of the present invention.
Description
Technical field
The present invention relates to plant cultivating method, and in particular to a kind of cultural method of cordyceps sinensis.
Background technology
Cordyceps sinensis also known as cordyceps sinensis, are a kind of China's usual rare tonic herbs among the people, and careless bacterium is most all parasitic
It is the provincialism of its growth and breeding, periodically extremely obvious in insect bodies, general integrated distribution high mountain extremely and snow suede with
On, or in more cold area.But also there is the footprint of cordyceps sinensis in torrid areas, be more common in spring and autumn.Due to cordyceps sinensis life
Power is strong, and to the strong adaptability of ecological environment, parasitic caste is more in addition, and parasitic insect is largely with plant
Leaf, root be food, so in forest and vegetation cordyceps sinensis occur it is most.Because its distribution is wide, therefore its title is different.Such as winter
Worm summer grass, Sichuan, Gansu are referred to as cordyceps sinensis or cordyceps, and Yunnan is then referred to as Cordyceps sinensis, and Tibet custom is referred to as refined miscellaneous
Roll cloth, China also some area cordyceps sinensis troat the worm summer grass.
Research shows that, containing abundant cordycepic acid in cordyceps sinensis, the nutriment such as cordycepin and each amino acid has
Blood vessel is expanded, it is calm, resist all kinds of bacteriums, effect of hypotensive.It is used for treating pulmonary tuberculosis now, old man's stomach cold is weak, puerpera
And old man's anaemia is weak, impotence of passing out semen, night sweat, soreness of waist disease, neurasthenia is empty long after being ill, the illness such as haematemesis, and cordyceps sinensis agent is also
Alopecia areata etc. can be controlled.Since the eighties, cordyceps sinensis can be suppressed the ability of tumour for raising human body by China, and strengthen people
The immunologic function of body.At present, worldwide, the cancer-resisting substance one that cordyceps sinensis has generally been gazed at.Current cordyceps sinensis is big
Many fields itself, if a kind of method of people's cultivation cordyceps sinensis can be provided to its a large amount of cultivation, can be greatly increased the winter
Worm summer grass is used for health, the possibility of health care.
The content of the invention
Based on above-mentioned condition, the present invention provides a kind of artificial cultivating method of cordyceps sinensis.Supported by of the present invention
Method is grown, good cordyceps sinensis can be cultivated.
Specifically, the present invention provides a kind of cultural method of cordyceps sinensis, and it comprises the steps：
（1）Prepare spore suspension：By the parts by weight of mealy potato 220, the parts by weight of white sugar 21, the parts by weight of potassium dihydrogen phosphate 3, magnesium sulfate
1.5 parts by weight, vitamin B1210 parts by weight, the parts by weight of production increasing agent 0.5, which are well mixed after high-temperature sterilization, obtains fluid nutrient medium, will
Cordyceps sinensis spore is placed in the fluid nutrient medium, is fermented 10 days in 20-23 DEG C of lucifuge, spore suspension is made；
（2）The selection in place：Set up multilayer yoke, every layer spread 7 cm thicks sandy soil, sand moisture is maintained on 40%, soil
Covered with leaf or debris；
（3）Apply liquid cultivation：Choosing 2 makes the bat moth larvae of larval stage for host insect, dilutes spore suspension, then uniformly
It is applied on each host insect；By the host insect seed covering in step（2）Cultivated 3 months in described sandy soil.
Wherein, in step（3）In, spore suspension is diluted to 8% concentration.
In addition, step（3）Described in seed covering carried out under dark conditions.
The formation of cordyceps sinensis and biological characteristic:Cordyceps assists bacterium.Son, which can be formed, after its thalline is ripe assists spore.Spore dissipates
Propagate, fall on suitable insect bodies with the wind after hair, particularly on the polypide of larva, just start growth and form mycelium, mycelia
The constantly growth of body one side, simultaneously starts to develop into polypide body, then insect is just by this fungal infection.If insect eats
Leaf with this kind of fungi, fungi can be also entered in polypide by the mouthpart of insect, with polypide in-vivo tissue and each organ
The matter and energy source grown as it, finally just will all be decomposed inside polypide, so as to the worm in fatal position.Fungi
Mycelium is by constantly development and breaks up, appearance that is final ripe and stretching out worm shell, and form a kind of fungi and polypide shell
The organism coexisted.Due to fungi outer first portion like grass thus be named referred to as " cordyceps sinensis ".
Cordyceps sinensis it is ecological complex, but depend primarily on the ecological environment of parasitic insect, this ecological environment very great Cheng
By temperature, humidity, the influence of illumination and air and other many natures and anthropic factor directly or indirectly on degree.In detail
Textual criticism and the ecological environment of investigation cordyceps sinensis, training is introduced a fine variety and artificial cultivation cordyceps sinensis is particularly important for artificial.On the Northern Hemisphere, mostly
Number cordyceps sinensis was mostly occurred between the 6-8 months, was common in birth woods leeward on the sunny side or scondary forest, occurred light application ratio in place in cordyceps sinensis
More sufficient, air and ground moistening, the yield of cordyceps sinensis increase with the increase of height, but with the increase of the mountain region gradient
It is relative to reduce.Sunny, displacement is good and in the higher vegetation of relative air humidity, this period in the Beginning of summer to the Summer Solstice
Interior, most easily harvesting draws grass to worm.
Aweto mycelium host insect is to belong to phosphorus wing order bat moth larvae, and the worm is typically grown in height above sea level 3650
The alpine meadow area of rice to 4250 meters, this band is in cordyceps sinensis growth period climate variability, and Chang Youyu, temperature is native in 0-23 degree
Earth humidity is 40-60%, and environment is moist.
The growing environment of present invention simulation cordyceps sinensis, is cultivated cordyceps sinensis, according to the cultural method of the present invention,
Cordyceps sinensis can largely be cultivated.
Embodiment
Technical scheme is described in detail with reference to embodiment, but the present invention is not limited to this.
Embodiment
（1）Prepare spore suspension：By 220 grams of mealy potato, 21 grams of white sugar, 3 grams of potassium dihydrogen phosphate, 1.5 grams of magnesium sulfate, dimension
Raw element B1210 grams, after 0.5 gram of well mixed high-temperature sterilization of production increasing agent fluid nutrient medium.By the cordyceps sinensis spore of collection, put
Put in this fluid nutrient medium and to be fermented 10 days in 20-23 DEG C of lucifuge, spore suspension is made.
（2）Select place：Interior sets multilayer yoke, every layer 7 cm thicks are spread with bamboo clappers sandy soil, sand moisture is maintained at
40% or so, covered with leaf or debris on soil.
（3）Apply liquid cultivation：2 are taken to make larval stage bat moth larvae.Spore suspension is diluted to 8% concentration, then
Uniformly it is applied on each bat moth larvae.By the larva seed covering in step（2）Cultivated 3 months in described sandy soil.
Claims (3)
1. a kind of cultural method of cordyceps sinensis, it is characterised in that comprise the steps：
（1）Prepare spore suspension：By the parts by weight of mealy potato 220, the parts by weight of white sugar 21, the parts by weight of potassium dihydrogen phosphate 3, magnesium sulfate
1.5 parts by weight, vitamin B1210 parts by weight, the parts by weight of production increasing agent 0.5, which are well mixed after high-temperature sterilization, obtains fluid nutrient medium, will
Cordyceps sinensis spore is placed in the fluid nutrient medium, is fermented 10 days in 20-23 DEG C of lucifuge, spore suspension is made；
（2）The selection in place：Set up multilayer yoke, every layer spread 7 cm thicks sandy soil, sand moisture is maintained on 40%, soil
Covered with leaf or debris；
（3）Apply liquid cultivation：Choosing 2 makes the bat moth larvae of larval stage for host insect, dilutes spore suspension, then uniformly
It is applied on each host insect；By the host insect seed covering in step（2）Cultivated 3 months in described sandy soil.
2. cultural method according to claim 1, it is characterised in that in step（3）In, spore suspension is diluted to
8% concentration.
3. cultural method according to claim 1, it is characterised in that step（3）Described in seed covering under dark conditions
Carry out.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710366920.9A CN107125026A (en) | 2017-05-23 | 2017-05-23 | A kind of cultural method of cordyceps sinensis |
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| CN201710366920.9A CN107125026A (en) | 2017-05-23 | 2017-05-23 | A kind of cultural method of cordyceps sinensis |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109566540A (en) * | 2018-05-22 | 2019-04-05 | 重庆市中药研究院 | A kind of artificial culturing method for cultivating cordyceps sinensis using insect |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1414091A (en) * | 2001-10-26 | 2003-04-30 | 李健 | New technology of culturing cordyceps sinensis using insect |
| CN101627701A (en) * | 2009-08-14 | 2010-01-20 | 郑杰辉 | Method for breeding cordyceps militars strain |
| CN103299821A (en) * | 2012-03-08 | 2013-09-18 | 黄友鹰 | Original ecological cordyceps sinensis high yielding and breeding method |
| CN103483037A (en) * | 2013-09-05 | 2014-01-01 | 东方中科生命科学有限责任公司 | Cordyceps liquid culture medium and application thereof |
-
2017
- 2017-05-23 CN CN201710366920.9A patent/CN107125026A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1414091A (en) * | 2001-10-26 | 2003-04-30 | 李健 | New technology of culturing cordyceps sinensis using insect |
| CN101627701A (en) * | 2009-08-14 | 2010-01-20 | 郑杰辉 | Method for breeding cordyceps militars strain |
| CN103299821A (en) * | 2012-03-08 | 2013-09-18 | 黄友鹰 | Original ecological cordyceps sinensis high yielding and breeding method |
| CN103483037A (en) * | 2013-09-05 | 2014-01-01 | 东方中科生命科学有限责任公司 | Cordyceps liquid culture medium and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109566540A (en) * | 2018-05-22 | 2019-04-05 | 重庆市中药研究院 | A kind of artificial culturing method for cultivating cordyceps sinensis using insect |
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Application publication date: 20170905 |