CN109566540A - A kind of artificial culturing method for cultivating cordyceps sinensis using insect - Google Patents
A kind of artificial culturing method for cultivating cordyceps sinensis using insect Download PDFInfo
- Publication number
- CN109566540A CN109566540A CN201811373266.5A CN201811373266A CN109566540A CN 109566540 A CN109566540 A CN 109566540A CN 201811373266 A CN201811373266 A CN 201811373266A CN 109566540 A CN109566540 A CN 109566540A
- Authority
- CN
- China
- Prior art keywords
- cordyceps sinensis
- temperature
- module
- insect
- larva
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001248610 Ophiocordyceps sinensis Species 0.000 title claims abstract description 72
- 241000238631 Hexapoda Species 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000012258 culturing Methods 0.000 title claims abstract description 12
- 230000003071 parasitic effect Effects 0.000 claims abstract description 16
- 241000382353 Pupa Species 0.000 claims description 15
- 235000013312 flour Nutrition 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 12
- 239000002689 soil Substances 0.000 claims description 12
- 241001018099 Alloteropsis Species 0.000 claims description 8
- 235000017898 Digitaria ciliaris Nutrition 0.000 claims description 8
- 241000196324 Embryophyta Species 0.000 claims description 7
- 238000009395 breeding Methods 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 241001237160 Kallima inachus Species 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- 241000209140 Triticum Species 0.000 claims description 6
- 235000021307 Triticum Nutrition 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 238000004378 air conditioning Methods 0.000 claims description 6
- 230000001488 breeding effect Effects 0.000 claims description 6
- 239000005321 cobalt glass Substances 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 239000000428 dust Substances 0.000 claims description 6
- 239000003344 environmental pollutant Substances 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 231100000719 pollutant Toxicity 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 229940088594 vitamin Drugs 0.000 claims description 6
- 229930003231 vitamin Natural products 0.000 claims description 6
- 235000013343 vitamin Nutrition 0.000 claims description 6
- 239000011782 vitamin Substances 0.000 claims description 6
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 6
- 102000002322 Egg Proteins Human genes 0.000 claims description 5
- 108010000912 Egg Proteins Proteins 0.000 claims description 5
- 210000004681 ovum Anatomy 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000003860 storage Methods 0.000 claims description 4
- 238000009412 basement excavation Methods 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 238000004364 calculation method Methods 0.000 claims description 3
- 230000012447 hatching Effects 0.000 claims description 3
- 239000003864 humus Substances 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 235000015099 wheat brans Nutrition 0.000 claims description 3
- 235000013601 eggs Nutrition 0.000 claims 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 abstract description 5
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 239000002126 C01EB10 - Adenosine Substances 0.000 abstract description 2
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 abstract description 2
- 241000190633 Cordyceps Species 0.000 abstract description 2
- 229960005305 adenosine Drugs 0.000 abstract description 2
- 150000001413 amino acids Chemical class 0.000 abstract description 2
- 230000033228 biological regulation Effects 0.000 abstract description 2
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 abstract description 2
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 229930195725 Mannitol Natural products 0.000 abstract 1
- 235000010355 mannitol Nutrition 0.000 abstract 1
- 239000000594 mannitol Substances 0.000 abstract 1
- 230000008901 benefit Effects 0.000 description 3
- 206010011224 Cough Diseases 0.000 description 2
- 206010062717 Increased upper airway secretion Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 208000026435 phlegm Diseases 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241001480006 Clavicipitaceae Species 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000330899 Hepialus Species 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 208000013116 chronic cough Diseases 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000000629 knee joint Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Inorganic Chemistry (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to medicinal material culture technique fields, a kind of artificial culturing method that cordyceps sinensis is cultivated using insect is disclosed, the artificial culture system for cultivating cordyceps sinensis using insect includes: larva culture module, nutriment mixing module, parasitic module, temperature and Humidity Control module, desiccation module.The model of temperature and Humidity Control module, temperature and Humidity Control module temperature and humidity adjustment of the invention, accurately controls the temperature and humidity of cordyceps sinensis, so that cordyceps sinensis is cultivated under extremely suitable environment, improves the quality and yield of cordyceps sinensis.The present invention cultivates the method that module cultivates aweto host bat moth larvae by larva, and survival rate is high；The present invention can be improved cordyceps sinensis quality simultaneously, and adenosine, mannitol, cordycepin, amino acid in Fermented Cordyceps etc. meet the standard regulation of cordyceps sinensis, and cordycepic acid content is also higher than cordyceps sinensis.
Description
Technical field
The invention belongs to medicinal material culture technique field more particularly to a kind of artificial cultures that cordyceps sinensis is cultivated using insect
Method.
Background technique
Cordyceps sinensis, Chinese medicine name.For Clavicipitaceae plant aweto Cordycepssinensis (BerK.) Sacc.
The complex of the corpse of parasitic stroma and larva on the larvae of batrachidae insects.With kidney tonifying benefit lung, hemostasis and phlegm effect.
Cure mainly impotence and seminal emission, soreness of waist and knee joint, chronic cough and dyspnea of deficiency type, overstrain cough phlegm blood.However, existing bat moth larva survival rate is low；Worm simultaneously
Grass product matter is low, and cordycepic acid content is few.
In conclusion problem of the existing technology is:
(1) existing bat moth larva survival rate is low；Cordyceps sinensis quality is low simultaneously, and cordycepic acid content is few.
(2) that there are Temperature and Humidity Controls is inaccurate for existing cordyceps sinensis breeding method, leads to the low output of cordyceps sinensis, product
Matter is low.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of artificial cultures that cordyceps sinensis is cultivated using insect
Method.
The invention is realized in this way a kind of artificial culture system for cultivating cordyceps sinensis using insect, described to utilize elder brother
Worm cultivate cordyceps sinensis artificial culture system include:
Larva cultivates module, connect with nutriment mixing module, for cultivating bat moth larvae；
Nutriment mixing module connect with larva culture module, parasitic module, be used for by weight, by corn flour 2~
2~3 parts of 2.5 parts, wheat flour or wheat bran, 1.5~2 parts of sucrose, 3-5 parts of glucose, 6~10 parts of yellow soil powder, smalt end slightly 12
2.5~3 parts of~15 parts, 0.6~1 part of cordyceps sinensis hypha,hyphae, 0.15~0.2 part of vitamin, lichens and dead leaf weeds powder,
Raw material is uniformly mixed, adjusting pH value is 6~7；
Parasitic module is connect with nutriment mixing module, temperature and Humidity Control module, is used for cordyceps sinensis fungi autoeciousness in bat
In Hepialus larva, cultivated on nutriment；
Temperature and Humidity Control module is connect, for controlling nutriment humidity 81~85% with parasitic module, desiccation module；Temperature
10~30 degrees Celsius, 12~20 degrees Celsius of preference temperature；
The temperature T of the temperature and Humidity Control moduleiControl formula:
In formula: Ti tIndicate the room temperature of t moment, DEG C；Indicate the outdoor temperature at t+1 moment, DEG C；C is equivalent heat
Hold, J/ DEG C；R is equivalent thermal resistance, DEG C/W；S is air-conditioning start and stop state variable, and 1 indicates starting of air conditioner, and 0 indicates that air-conditioning stops；Δt
For simulation time interval.
The model of the temperature and Humidity Control module temperature and humidity adjustment are as follows:
X (k+1)=Ajx(k)+Ad jx(k-1)+Bju(k)；
A in formulaj, Ad j, BjFor coefficient matrix, j=1,2 ..., 2m, X=[X1,X2] T be state variable, X1For greenhouse temperature
Degree, X2Chamber humidity, u are disturbance input amount, and label j indicates j-th of subsystem；
The model of temperature and Humidity Control module, temperature and Humidity Control module temperature and humidity adjustment of the invention, to the temperature of cordyceps sinensis
Humidity is accurately controlled, so that cordyceps sinensis is cultivated under extremely suitable environment, improves the quality and production of cordyceps sinensis
Amount.
The cordyceps sinensis reconstitution rate calculation formula are as follows:
In formula: m1For the quality of cordyceps sinensis drying sample, g；m2For cordyceps sinensis quality, g after rehydration；w0For the fresh winter
The initial wet basis moisture content of worm summer grass, g/g；w1For cordyceps sinensis drying sample wet basis moisture content, g/g.
Cordyceps sinensis reconstitution rate of the invention calculates, and the quality of cordyceps sinensis Related product is helped to improve, for enterprise
Development it is most important.
Another object of the present invention is to provide a kind of artificial culture systems that cordyceps sinensis is cultivated using insect
The artificial culturing method of cordyceps sinensis is cultivated using insect, the artificial culturing method for cultivating cordyceps sinensis using insect includes
Following steps:
Step 1 cultivates module by larva and cultivates bat moth larvae；
Step 2, by nutriment mixing module by corn flour, wheat flour, sucrose, glucose, yellow soil powder, smalt end slightly,
Cordyceps sinensis hypha,hyphae, vitamin, lichens are uniformly mixed with dead leaf weeds powder raw material；
Step 3, by parasitic module by cordyceps sinensis fungi autoeciousness on bat moth larvae, cultivated on nutriment；
Step 4 controls nutriment humidity 81~85% by temperature and Humidity Control module；10~30 degrees Celsius of temperature, be suitable for temperature
12~20 degrees Celsius of degree, and taken out by the cordyceps sinensis that desiccation module forms oneself, then desiccation, water content 6~7% enters
Library saves.
Further, the larva culture module cultural method is as follows:
Firstly, adopting pupa, the pupa of the wild bat moth in Alpine meadow area is acquired, is saved；
Secondly, acquisition breeding dust, collects the plateau fertile soil of worm pupa excavation area peripheral edge；
Then, sprout wings, mate, pupa is placed on equipped in the container humous of plateau, and pupa is located at the 2.5- under soil
3cm, and sprout wings at 10-12 DEG C in container all around with gauze, after sprouting wings into host's bat moth, 3-5d completes to mate and produce
Ovum；
Then, hatch, insect-taking ovum is placed in humus surface, puts constant incubator into and carry out being protected from light hatching, constant temperature incubation
Box temperature degree is 15 DEG C, relative humidity 85-90%, is hatched into larva；
Finally, raising, takes raising box, be put into breeding dust, larva is transferred in raising box, feed is put into, in 12-15
It DEG C is raised；
The air quality of the raising box calculates model are as follows:
In formula: IAQIp is the air quality separate index number of each individual event pollutant；IAQIHiFor the high-order critical value close with Cp
Corresponding air quality index；IAQILoFor air quality index corresponding with the low level critical value that Cp is close；BPHiTo be connect with Cp
Close high-order critical value；BPLoFor the low level critical value close with Cp；Cp is pollutant concentration value.
The air quality of raising box of the invention calculates model, and the air quality that can be accurately obtained in raising box is right
Play the role of in the cultivation for improving cordyceps sinensis to pass.
Another object of the present invention is to provide a kind of using the artificial culture side for cultivating cordyceps sinensis using insect
The cordyceps sinensis culture system of method.
Another object of the present invention is to provide a kind of using the artificial culture side for cultivating cordyceps sinensis using insect
The cordyceps sinensis bedstead cultivation system of method.
Advantages of the present invention and good effect are as follows: the present invention cultivates module by larva and cultivates aweto host bat
The method of moth larvae, survival rate are high；The present invention can be improved cordyceps sinensis quality simultaneously, adenosine in Fermented Cordyceps, sweet
Dew alcohol, cordycepin, amino acid etc. meet the standard regulation of cordyceps sinensis, and cordycepic acid content is also higher than cordyceps sinensis.
Detailed description of the invention
Fig. 1 is the artificial culturing method flow chart that cordyceps sinensis is cultivated using insect that the present invention implements to provide；
Fig. 2 is the artificial culture system structure diagram that cordyceps sinensis is cultivated using insect that the present invention implements to provide；
In figure: 1, larva cultivates module；2, nutriment mixing module；3, parasitic module；4, temperature and Humidity Control module；5, desiccation
Module.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
With reference to the accompanying drawing and specific embodiment is further described application principle of the invention.
As shown in Figure 1, it is provided by the invention using insect cultivate cordyceps sinensis artificial culturing method the following steps are included:
S101: module is cultivated by larva and cultivates bat moth larvae；
S102: by nutriment mixing module by corn flour, wheat flour, sucrose, glucose, yellow soil powder, smalt end slightly, the winter
Worm summer grass hypha,hyphae, vitamin, lichens are uniformly mixed with dead leaf weeds powder raw material；
S103: by parasitic module by cordyceps sinensis fungi autoeciousness on bat moth larvae, cultivated on nutriment；
S104: nutriment humidity 81~85% is controlled by temperature and Humidity Control module；10~30 degrees Celsius of temperature, preference temperature
12~20 degrees Celsius, and taken out by the cordyceps sinensis that desiccation module forms oneself, then desiccation, water content 6~7%, storage
It saves.
As shown in Fig. 2, the artificial culture system provided by the invention for cultivating cordyceps sinensis using insect includes: that larva is cultivated
Module 1, nutriment mixing module 2, parasitic module 3, temperature and Humidity Control module 4, desiccation module 5.
Larva cultivates module 1, connect with nutriment mixing module 2, for cultivating bat moth larvae；
Nutriment mixing module 2 connect with larva culture module 1, parasitic module 3, is used for by weight, by corn flour 2
2~3 parts of~2.5 parts, wheat flour or wheat bran, 1.5~2 parts of sucrose, 3-5 parts of glucose, 6~10 parts of yellow soil powder, smalt end are slightly
12~15 parts, 0.6~1 part of cordyceps sinensis hypha,hyphae, 0.15~0.2 part of vitamin, lichens and dead leaf weeds powder 2.5~3
Part, raw material is uniformly mixed, adjusting pH value is 6~7；
Parasitic module 3 is connect with nutriment mixing module 2, temperature and Humidity Control module 4, is used for cordyceps sinensis fungi autoeciousness
On bat moth larvae, cultivated on nutriment；
Temperature and Humidity Control module 4 is connect, for controlling nutriment humidity 81~85% with parasitic module 3, desiccation module 5；Temperature
10~30 degrees Celsius, 12~20 degrees Celsius of preference temperature of degree；
Desiccation module 5 is connect with temperature and Humidity Control module 4, and the cordyceps sinensis for forming oneself takes out, then desiccation,
Water content 6~7%, storage save.
Larva culture 1 cultural method of module provided by the invention is as follows:
Firstly, adopting pupa, the pupa of the wild bat moth in Alpine meadow area is acquired, is saved；
Secondly, acquisition breeding dust, collects the plateau fertile soil of appropriate worm pupa excavation area peripheral edge；
Then, sprout wings, mate, pupa is placed on equipped in the container humous of plateau, and pupa is located at the 2.5- under soil
3cm, and sprout wings at 10-12 DEG C in container all around with gauze, after sprouting wings into host's bat moth, 3-5d completes to mate and produce
Ovum；
Then, hatch, insect-taking ovum is placed in humus surface, puts constant incubator into and carry out being protected from light hatching, constant temperature incubation
Box temperature degree is 15 DEG C, relative humidity 85-90%, is hatched into larva；
Finally, raising, takes raising box, be put into breeding dust, larva is transferred in raising box, feed is put into, in 12-15
It DEG C is raised.
The temperature T of temperature and Humidity Control moduleiControl formula:
In formula: Ti tIndicate the room temperature of t moment, DEG C；Indicate the outdoor temperature at t+1 moment, DEG C；C is equivalent heat
Hold, J/ DEG C；R is equivalent thermal resistance, DEG C/W；S is air-conditioning start and stop state variable, and 1 indicates starting of air conditioner, and 0 indicates that air-conditioning stops；Δt
For simulation time interval.
The model of temperature and Humidity Control module temperature and humidity adjustment are as follows:
X (k+1)=Ajx(k)+Ad jx(k-1)+Bju(k)；
A in formulaj, Ad j, BjFor coefficient matrix, j=1,2 ..., 2m, X=[X1,X2] T be state variable, X1For greenhouse temperature
Degree, X2Chamber humidity, u are disturbance input amount, and label j indicates j-th of subsystem；
Cordyceps sinensis reconstitution rate calculation formula are as follows:
In formula: m1For the quality of cordyceps sinensis drying sample, g；m2For cordyceps sinensis quality, g after rehydration；w0For the fresh winter
The initial wet basis moisture content of worm summer grass, g/g；w1For cordyceps sinensis drying sample wet basis moisture content, g/g.
The air quality for raising box calculates model are as follows:
In formula: IAQIp is the air quality separate index number of each individual event pollutant；IAQIHiFor the high-order critical value close with Cp
Corresponding air quality index；IAQILoFor air quality index corresponding with the low level critical value that Cp is close；BPHiTo be connect with Cp
Close high-order critical value；BPLoFor the low level critical value close with Cp；Cp is pollutant concentration value.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (5)
1. a kind of artificial culture system for cultivating cordyceps sinensis using insect, which is characterized in that described to cultivate winter worm using insect
The artificial culture system of summer grass includes:
Larva cultivates module, connect with nutriment mixing module, for cultivating bat moth larvae；
Nutriment mixing module connect with larva culture module, parasitic module, be used for by weight, by 2~2.5 parts of corn flour,
2~3 parts of wheat flour or wheat bran, 1.5~2 parts of sucrose, 3-5 parts of glucose, 6~10 parts of yellow soil powder, slightly 12~15 parts of smalt end,
2.5~3 parts of 0.6~1 part of cordyceps sinensis hypha,hyphae, 0.15~0.2 part of vitamin, lichens and dead leaf weeds powder, by raw material
It is uniformly mixed, adjusting pH value is 6~7；
Parasitic module is connect with nutriment mixing module, temperature and Humidity Control module, is used for cordyceps sinensis fungi autoeciousness in bat moth
On larva, cultivated on nutriment；
Temperature and Humidity Control module is connect, for controlling nutriment humidity 81~85% with parasitic module, desiccation module；Temperature 10~
30 degrees Celsius, 12~20 degrees Celsius of preference temperature；
The temperature T of the temperature and Humidity Control moduleiControl formula:
In formula: Ti tIndicate the room temperature of t moment, DEG C；Indicate the outdoor temperature at t+1 moment, DEG C；C is equivalent thermal capacitance, J/
℃；R is equivalent thermal resistance, DEG C/W；S is air-conditioning start and stop state variable, and 1 indicates starting of air conditioner, and 0 indicates that air-conditioning stops；Δ t is emulation
Time interval.
The model of the temperature and Humidity Control module temperature and humidity adjustment are as follows:
X (k+1)=Ajx(k)+Ad jx(k-1)+Bju(k)；
A in formulaj, Ad j, BjFor coefficient matrix, j=1,2 ..., 2m, X=[X1,X2] T be state variable, X1For greenhouse temperature, X2Temperature
Room humidity, u are disturbance input amount, and label j indicates j-th of subsystem；
Desiccation module, connect with temperature and Humidity Control module, and the cordyceps sinensis for forming oneself takes out, then desiccation, water content 6
~7%, storage saves.
The cordyceps sinensis reconstitution rate calculation formula are as follows:
In formula: m1For the quality of cordyceps sinensis drying sample, g；m2For cordyceps sinensis quality, g after rehydration；w0For the fresh winter worm summer
Careless initial wet basis moisture content, g/g；w1For cordyceps sinensis drying sample wet basis moisture content, g/g.
2. a kind of artificial culture system for cultivating cordyceps sinensis using insect as described in claim 1 cultivates winter worm using insect
The artificial culturing method of summer grass, which is characterized in that the artificial culturing method that cordyceps sinensis is cultivated using insect includes following
Step:
Step 1 cultivates module by larva and cultivates bat moth larvae；
Step 2, by nutriment mixing module by corn flour, wheat flour, sucrose, glucose, yellow soil powder, smalt end slightly, winter worm
Summer grass hypha,hyphae, vitamin, lichens are uniformly mixed with dead leaf weeds powder raw material；
Step 3, by parasitic module by cordyceps sinensis fungi autoeciousness on bat moth larvae, cultivated on nutriment；
Step 4 controls nutriment humidity 81~85% by temperature and Humidity Control module；10~30 degrees Celsius of temperature, preference temperature 12
~20 degrees Celsius, and taken out by the cordyceps sinensis that desiccation module forms oneself, then desiccation, water content 6~7%, storage is protected
It deposits.
3. the artificial culturing method of cordyceps sinensis is cultivated using insect as claimed in claim 2, which is characterized in that the larva
It is as follows to cultivate module cultural method:
Firstly, adopting pupa, the pupa of the wild bat moth in Alpine meadow area is acquired, is saved；
Secondly, acquisition breeding dust, collects the plateau fertile soil of worm pupa excavation area peripheral edge；
Then, sprout wings, mate, pupa is placed on equipped in the container humous of plateau, and pupa is located at the 2.5-3cm under soil, and
In container all around with gauze, sprout wings at 10-12 DEG C, after sprouting wings into host's bat moth, 3-5d completes to mate and lay eggs；
Then, hatch, insect-taking ovum is placed in humus surface, puts constant incubator into and carry out being protected from light hatching, constant temperature incubation box temperature
Degree is 15 DEG C, relative humidity 85-90%, is hatched into larva；
Finally, raising, take raising box, be put into breeding dust, by larva be transferred to raising box in, be put into feed, 12-15 DEG C into
Row raising；
The air quality calculating side model of the raising box are as follows:
In formula: IAQIp is the air quality separate index number of each individual event pollutant；IAQIHiIt is corresponding with the close high-order critical value of Cp
Air quality index；IAQILoFor air quality index corresponding with the low level critical value that Cp is close；BPHiIt is close with Cp
High-order critical value；BPLoFor the low level critical value close with Cp；Cp is pollutant concentration value.
4. a kind of winter using the artificial culturing method for utilizing insect to cultivate cordyceps sinensis described in claim 2~3 any one
Worm summer grass culture system.
5. a kind of winter using the artificial culturing method for utilizing insect to cultivate cordyceps sinensis described in claim 2~3 any one
Worm summer grass bedstead cultivation system.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810493526.6A CN108668781A (en) | 2018-05-22 | 2018-05-22 | A kind of artificial culturing method for cultivating cordyceps sinensis using insect |
| CN2018104935266 | 2018-05-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109566540A true CN109566540A (en) | 2019-04-05 |
Family
ID=63807710
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810493526.6A Withdrawn CN108668781A (en) | 2018-05-22 | 2018-05-22 | A kind of artificial culturing method for cultivating cordyceps sinensis using insect |
| CN201811373266.5A Pending CN109566540A (en) | 2018-05-22 | 2018-11-19 | A kind of artificial culturing method for cultivating cordyceps sinensis using insect |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810493526.6A Withdrawn CN108668781A (en) | 2018-05-22 | 2018-05-22 | A kind of artificial culturing method for cultivating cordyceps sinensis using insect |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN108668781A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110476895A (en) * | 2019-08-12 | 2019-11-22 | 北京同仁堂健康药业（青海）有限公司 | A kind of subculture pupa automatic nursing and emerging adult collection device and method |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109380181A (en) * | 2018-11-13 | 2019-02-26 | 宜都长江机械设备有限公司 | A kind of bat moth incubator and the method that bat moth is cultivated using bat moth incubator |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1414091A (en) * | 2001-10-26 | 2003-04-30 | 李健 | New technology of culturing cordyceps sinensis using insect |
| CN102405763A (en) * | 2010-09-26 | 2012-04-11 | 王美才 | Cultivating method of aweto |
| CN102960183A (en) * | 2012-10-31 | 2013-03-13 | 贵州神奇集团控股有限公司 | Artificial culture method for Cordyceps sinensis and Cordyceps sinensis product |
| CN103918480A (en) * | 2014-04-11 | 2014-07-16 | 钱志强 | Artificial culturing method for cordyceps sinensis |
| CN104322451A (en) * | 2014-08-20 | 2015-02-04 | 广东省昆虫研究所 | Manual low-altitude raising method for host insect hepialidae of ophiocordyceps sinensis |
| CN105941340A (en) * | 2016-05-25 | 2016-09-21 | 浙江农林大学 | An artificial culture method for ghost moth larvae, the hosts of ophiocordyceps sinensis |
| CN107125026A (en) * | 2017-05-23 | 2017-09-05 | 高必红 | A kind of cultural method of cordyceps sinensis |
-
2018
- 2018-05-22 CN CN201810493526.6A patent/CN108668781A/en not_active Withdrawn
- 2018-11-19 CN CN201811373266.5A patent/CN109566540A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1414091A (en) * | 2001-10-26 | 2003-04-30 | 李健 | New technology of culturing cordyceps sinensis using insect |
| CN102405763A (en) * | 2010-09-26 | 2012-04-11 | 王美才 | Cultivating method of aweto |
| CN102960183A (en) * | 2012-10-31 | 2013-03-13 | 贵州神奇集团控股有限公司 | Artificial culture method for Cordyceps sinensis and Cordyceps sinensis product |
| CN103918480A (en) * | 2014-04-11 | 2014-07-16 | 钱志强 | Artificial culturing method for cordyceps sinensis |
| CN104322451A (en) * | 2014-08-20 | 2015-02-04 | 广东省昆虫研究所 | Manual low-altitude raising method for host insect hepialidae of ophiocordyceps sinensis |
| CN105941340A (en) * | 2016-05-25 | 2016-09-21 | 浙江农林大学 | An artificial culture method for ghost moth larvae, the hosts of ophiocordyceps sinensis |
| CN107125026A (en) * | 2017-05-23 | 2017-09-05 | 高必红 | A kind of cultural method of cordyceps sinensis |
Non-Patent Citations (4)
| Title |
|---|
| 吴中华 等: "枸杞分段式变温热风干燥特性及干燥品质", 《农业工程学报》 * |
| 周磊 等: "聚合空调负荷的温度调节方法改进及控制策略", 《中国电机工程学报》 * |
| 潘本锋 等: "环境空气质量指数计算方法与分级方案比较", 《中国环境监测》 * |
| 秦琳琳等: "基于灰色预测模型的温室温湿度系统建模与控制 ", 《农业工程学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110476895A (en) * | 2019-08-12 | 2019-11-22 | 北京同仁堂健康药业（青海）有限公司 | A kind of subculture pupa automatic nursing and emerging adult collection device and method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108668781A (en) | 2018-10-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102405763B (en) | Cultivating method of aweto | |
| CN102440149B (en) | Method for breeding cordyceps militaris by utilizing silkworm larvae | |
| CN103385220B (en) | Scale breeding method for encarsia formosa | |
| CN105941340B (en) | Artificial culture method of cordyceps sinensis host hepialus armoricanus larvae | |
| CN104585135A (en) | Overwintering breeding method for aphidiusgifuensis in northern area | |
| CN103141394A (en) | Method for culturing adventitious roots of oplopanax elatus Nakai by utilizing bioreactor | |
| CN108782009A (en) | A kind of hickory chick method for increasing | |
| CN105191855A (en) | Method for manually hatching chickens inside soil pit | |
| CN105075667A (en) | Method used for artificially cultivating Chinese caterpillar fungus in low altitude region | |
| CN109042541A (en) | A method of grub is cultivated using fermented cow dung | |
| CN106358755A (en) | Interplanting cultivation method of lentinula edodes, morchella vulgarises and kudzuvine roots | |
| CN103141447B (en) | Method for artificially culturing cricket in soilless and temperature-control manner | |
| CN109566540A (en) | A kind of artificial culturing method for cultivating cordyceps sinensis using insect | |
| CN102939855B (en) | The cultivating method of imitative wild cordyceps militaris | |
| CN111631191B (en) | Method for cultivating cordyceps sinensis in original production area | |
| CN104365375A (en) | Indoor pulverization stimulation fruiting cultivation method for abalone mushrooms, agrocybe cylindracea and lentinus giganteu | |
| CN106942159B (en) | Intelligent ladybug culture device and culture method thereof | |
| CN107372363A (en) | A kind of facility that can improve cultivation black soldier flies adult density in green house of vegetables | |
| CN103340091B (en) | Method for artificially cultivating xuefengensis | |
| CN101423801A (en) | Artificial culture method of Chinese caterpillar fungus | |
| CN105838621B (en) | A kind of culture solution and breeding method of grifola frondosus liquid spawn | |
| CN108184773B (en) | Method for improving survival rate of low-altitude bred bat moth larvae | |
| CN105379682A (en) | Coridius chinensis large-scale artificial breeding technique | |
| CN106508813A (en) | Imitation wild propagation method of mysorethorn moths | |
| CN108934765A (en) | A kind of cultural method of semi-wild red ganoderma |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190405 |
|
| RJ01 | Rejection of invention patent application after publication |