CN107056441A - A kind of preparation method of the bio-feritlizer containing willow leaf - Google Patents

A kind of preparation method of the bio-feritlizer containing willow leaf Download PDF

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Publication number
CN107056441A
CN107056441A CN201710234779.7A CN201710234779A CN107056441A CN 107056441 A CN107056441 A CN 107056441A CN 201710234779 A CN201710234779 A CN 201710234779A CN 107056441 A CN107056441 A CN 107056441A
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fermentation
willow leaf
parts
bio
willow
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徐会连
薛鹏飞
陈晋銮
苏亚平
杨瑞芳
车树岗
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Shandong Bohua Highly-Efficient Ecological And Agricultural Technology Co Ltd
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Shandong Bohua Highly-Efficient Ecological And Agricultural Technology Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/04—Double-superphosphate; Triple-superphosphate; Other fertilisers based essentially on monocalcium phosphate
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/70—Controlling the treatment in response to process parameters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Fertilizers (AREA)

Abstract

The invention belongs to field of microbial fermentation there is provided a kind of preparation method of the bio-feritlizer containing willow leaf, the leaf of fresh willow, willow is smashed to pieces, squeezed the juice, extracting the new bio medicine bacterial manure for making liquid by this method.The present invention mainly uses microbial fermentation technology, by the multiple fermentation technique of original creation, and uses newest bio-feritlizer formula, really realizes the unification of willow leaf medicine, bacterium, fertilizer, microorganism special efficacy and nutrition special efficacy.

Description

A kind of preparation method of the bio-feritlizer containing willow leaf
Technical field
The invention belongs to biological fermentation field, a kind of making side of the bio-feritlizer containing willow leaf is specifically provided Method.
Background technology
Agricultural chemicals and chemical fertilizer are two kinds of the most frequently used chemical products in daily agricultural production, and it is largely used, not only Polluted Soil Earth, water body, while causing Practice for Pesticide Residue in Agricultural Products severe overweight, some organic fertilizer are also because of the agricultural chemicals containing high concentration, and its is residual Thing is stayed still to have huge injury to human body, to reduce harm of the chemical pesticide to environment and agricultural product, some areas use biological agriculture Medicine replaces chemical pesticide, but presently commercially available biological pesticide is mainly the effective active composition that extract by solvents is utilized from plant, Its composition is more single, and production equipment is relative complex, and partial solvent is also easy to produce anti-medicine to human health in use Property, influence insecticidal effect.
Willow, willow are commonly used to build pollard woods in north of China, are the trees that China ancients recognize and largely transplanted earliest One of kind.In willow leaf, substantial amounts of acetylsalicylic acid is contained particularly in tender leaf and spray, that is, it is well known The source of aspirin.Aspirin is a kind of medically common non-prescribed medicine.But it is agriculturally serving many purposes, it The drought-resistant ability of crops can be improved, percentage of seedgermination is improved, prevents corps diseases, crop fruit drop is reduced, improved Crops quality, improves crop yield etc..
Biological medicament bacterial manure is to be formulated the composition with special medicinal function according to a certain percentage with microorganism, nutrient Mix, and by certain technology by fertilizer, microorganism, medicinal function stable components in specific compound system and The new bio medicine bacterial manure of formation.
Application No.：201210463557.X the Chinese patent with 201210486876.2, individually discloses a kind of konjaku With the special medicated fertilizer of paddy rice, water fruits and vegetables energy crops are not particularly suited for as medicine fertilizer；Application No. 201110458631.4 Chinese patent discloses a kind of organic biological medical fertilizer, but it uses potassium hydroxide and rare earth element in the feed, and preparation technology is needed Use ion exchange tower, raw material fails all using organic matter and preparation technology is cumbersome, unsuitable large-scale promotion application.
The content of the invention
There is provided a kind of bio-feritlizer containing willow leaf and its making for deficiency of the invention for the presence of above-mentioned technology The leaf of fresh willow, willow is smashed to pieces, squeezed the juice, extracting the new bio medicine bacterial manure for making liquid by method, this method.This Invention mainly uses microbial fermentation technology, by the multiple fermentation technique of original creation, and uses newest bio-feritlizer formula, very Just realizing the unification of willow leaf medicine, bacterium, fertilizer, microorganism special efficacy and nutrition special efficacy.
The present inventor in fact provides one kind and contains the new bio medicine bacterial manure of leaf containing willow, is a kind of new Liquid biological medicament bacterial manure material, therefore new bio medicine bacterial manure material is prepared by raw material of fresh willow leaf, can not only become give up into Treasured, makes willow leaf resource go on green circulatory development, and be the new work of the exploitation injection of willow willow resource Power, with wide market development prospect.
The concrete technical scheme that the present invention is provided is as follows：
A kind of preparation method of the bio-feritlizer containing willow leaf, including the step such as one time fermentation, secondary fermentation, specifically Step is as follows：
1) fresh willow, willow spray and tender leaf is collected；
2) by step 1) obtained material carries out mashing crushing；
3) one time fermentation：By step 2) obtained raw material packed with 3 layers of gauze, and making turns into willow leaf bag, and will It is added in fermentation tank, has the following raw material according to mass percent in fermentation tank：
Willow leaf：50-70%；
Molasses：5-10%；
Urea：0.5-2%；
Probiotics strain：3-7%；
Water：Surplus；
4) according to step 3) ratio, the addition of each component is finished and starts ventilation and stirs, the requirement of ventilation is energy Seethe the liquid in fermentation tank, ventilation time is 20-30min；
5) after the completion of divulging information, fermentation tank is sealed, under the conditions of 35-40 DEG C, anaerobic fermentation 4-5 days；
6) after the completion of anaerobic fermentation, fermentation tank is opened, divulge information 40-60min, carries out aerobic fermentation at normal temperatures afterwards, when Between 15-20 days；
7) monitoring raw material juice pH value change at any time, when pH is dropped to below 4, terminates aerobic fermentation；
8) by step 7) fermenting raw materials liquid carry out filtered 80 mesh sieve, obtain clear liquid, that is, obtain willow leaf bacterial manure mother liquor；
9) by step 8) obtained willow leaf bacterial manure mother liquor is transferred in bucket, and lucifuge room temperature preservation is standby；
10) secondary fermentation
According to parts by weight, each component composition is as follows：
Willow leaf bacterial manure mother liquor：70-90 parts；
Molasses：5-8 parts；
Urea：3-8 parts；
Wheat bran water：1-2 parts；
Compound amino acid：0.5-1 parts；
Composite trace element：0.2-0.5 parts；
Potassium dihydrogen phosphate：1-2 parts；
Calcium dihydrogen phosphate：4-7 parts；
Magnesium sulfate：1-2 parts；
Mannitol：0.5-2 parts；
11) above material is proportionally added in fermentation tank, fully dissolving, and stirring；
12) by material heat temperature raising, 40-45 DEG C is reached to temperature of charge, air is passed through, 30min is sufficiently stirred for；
13) after the completion of stirring, according to the 5-10% of material cumulative volume, probiotics strain is added, inoculation is completed, air is opened Stirring, time 30-60min；
14) step 13) after the completion of, fermentation tank is sealed, temperature control is in 40-45 DEG C, anaerobic fermentation 24-36h；
15) step 14 is completed) after, fermentation tank is opened, 30min is stirred in ventilation, and temperature control is in 25-30 DEG C, aerobic fermentation 4-5 days, until one layer of white mycoderm occurs in zymotic fluid top layer；
16) occur after white mycoderm, zymotic fluid is poured out into bucket from fermentation tank, lucifuge room temperature preservation, no It is tight with the lid sealing bucket, a fixed gap is left, afterripening fermentation process is carried out；
17) detection pH changes, treat that pH is down to less than 4, afterripening fermentation is completed, and the bio-feritlizer containing willow leaf has made Into.
Of the present invention is fresh willow leaf, mainly not rot, is not gone mouldy for standard；In crushing When fresh willow leaf may lose a part liquid, but occur this part at any time liquid when, can be collected, With fermentation bag, one piece is placed in fermentation process, improves original utilization rate；
Above-mentioned used all probiotics are that profit Kang Yuan EM stostes are purchased from Sino-Japan joint Linyi Yikang organic agriculture Technology Park Co., Ltd；
Step 7) in if aerobic fermentation was by 20 days, pH value is still not up to less than 4, still terminates aerobic fermentation process, To ensure ferment effect；
After one time fermentation, acetylsalicylic acid, crude fibre, crude protein, crude fat and multiple element in willow leaf All entered in the way of being merged with organic acid in mother liquor, hence into secondary fermentation；And in secondary fermentation, except mentioned component It is extracted, organic matter, a variety of effective medicinal ingredients in willow leaf, such as flavonoids, amino acids material, also has big The organic matter of amount can be also extracted, into liquid bio-fertilizer.
The step 10) in wheat bran water be：Wheat bran is soaked according to the warm water of 5 times of wheat bran volumes 5-6 hours, takes supernatant ；Why use wheat bran water, be because wheat bran in containing microorganism start growth and breeding necessary to nutriment, including Amino acid, vitamin, growth factor etc., and by above-mentioned warm water immersion after wheat bran water in it is that this moieties are completely molten Go out, the effect for the breeding for starting microorganism within the most short time can be reached, therefore inventor is preferred to use above-mentioned parts by weight Wheat bran water add.
The trace element and amino acid are all finished product of the buying from market, such as：Compound amino acid supplier is Chengdu ammonia Base acid chelating Bioisystech Co., Ltd, is 18 kinds of compound amino acid compound powder products, and content is >=98%；It is combined micro member The composite trace element that plain supplier provides for U.S. North America Chemical Group, content is >=99%；The supplier of potassium dihydrogen phosphate For Guangzhou Run Zhan Chemical Co., Ltd.s, content >=98%；The supplier of calcium dihydrogen phosphate is that Zhengzhou east is limited up to chemical products Company, content >=98%；The supplier of magnesium sulfate is Guan Bo chemical products Co., Ltd of Pingdingshan City, content >=98%；Sweet dew The supplier of alcohol is Hua Tian sugar alcohols Co., Ltd of Shouguang City, content >=98%.The said goods are that city is available；
Contain a kind of effective medicinal ingredient-salicylic acid in willow leaf because willow leaf contains, this is aspirin After main active substances, the method using the present invention：Fermentation process is the process of the metabolism and breeding of microorganism, at this During microbes by the use of the C and N in matrix as nutriment, synthesize a variety of organic acids, amino acid and polypeptides matter, So be achieved that the secondary utilization of nitrogen source and carbon source in willow leaf, what is more important in this process, in willow Acetylsalicylic acid can be completely broken down out, and in organic acidity environment in, keep its activity；This side is used simultaneously The source for the acetylsalicylic acid that method is obtained is pure plant origin, is not the material of chemical synthesis, therefore, is easy to after administration It is absorbed by crops and greatly improves utilization ratio, and the drug effect of acetylsalicylic acid can then be fully played so that fertilizer Trophism and protection effect have obtained compatibility.
Inventor is adjusted to whole raw material proportioning, so that the nutritional ingredient in whole fertilizer system can unite One；While the purpose for adding sorbierite is exactly for the chelating of trace element in promoting in liquid fertilizer system, it is micro in promotion The uptake of element and the transport velocity in plant tissue；Magnesium sulfate this material is also added in the present invention, because Improving the content of exogenous magnesium can promote the physiological metabolism of crop to act on, and promote plant growth.Given birth to existing normal fermentation The Water soluble fertilizer of production is compared with microbial-bacterial fertilizer, the characteristics of present invention has following：
1st, raw material is selected：Compared with existing liquid organic fertilizer, willow leaf Liquid Fertilizer, which contains, a variety of in willow leaf to be had The medicinal ingredient of effect, wherein, 35 kinds of flavone compound；17 kinds of amino acid and various trace elements, are natural fertilizer materials, Liquid extraction fermentation and solid fermentation by the present invention, can realize comprehensive utilization, the waste caused is seldom；
2nd, fermentation process：The advantage of secondary fermentation is the effect point that the nutritional ingredient of different shape can be passed through into microorganism The small molecule form that solution can absorb into root system of plant；The advantage of another aspect is exactly to pass through secondary fermentation technology, will be secondary The raw materials such as mannitol are added, are sugar alcohol chelating activity state by inorganic constituents activation, neither contaminated soil, the thing of soil is not destroyed Characteristic is managed, can be quickly absorbed and used by plants again；
3rd, complex microbial community is make use of, during spontaneous fermentation, by open fermentation process, by aerobic And anaerobic fermentation process, the abundant microorganism species in nature can be bred in liquid fertilizer system, difference can be accomplished Probiotics mutualism, have a great advantage using single microflora fermentation than prior art, and selected compound micro- Biological flora is the optimal flora selected after long-term a large amount of trials；
4th, low ph value ensures effect：The pH that finished product contains the bio-feritlizer of willow leaf is removed in 4.0 or so, this environment The profitable strain that the present invention is obtained can survive outer, and pathogenic bacteria and pathogen are all unable to normal life, can effectively suppress miscellaneous bacteria Growth, it is ensured that the advantage of microorganism species；Flavone compound more can effectively be absorbed and profit under conditions of low PH With, it is ensured that its effect.
5th, the control of temperature：Temperature control during the fermentation, different fermentation temperatures, mesh are used in the different stages Be exactly to ensure the optimum growth temp of microorganism, it is ensured that its growth activity.
In summary, this law is bright provides a kind of preparation method of the new bio medicine bacterial manure material containing willow leaf, should Method is smashed to pieces from fresh willow leaf, squeezed the juice, extracting the biological medicament bacterial manure for making liquid.This patent mainly solves willow The preparation method of leaf liquid medicine bacterial manure, the technology mainly used is microbial fermentation technology.By the multiple fermentation technique of original creation, And newest fertilizer formula is used, really realize the unification of willow leaf bacterial manure microorganism special efficacy and nutrition special efficacy.
Embodiment
The embodiment of form, does further specifically to the above of the invention by the following examples It is bright, but this should not be interpreted as to the scope of above-mentioned theme of the invention be only limitted to following example.It is all to be based on the above of the present invention The technology realized belongs to the scope of the present invention, unless otherwise specified, complete using conventional prior in following embodiments Into.
Embodiment 1
A kind of preparation method of the bio-feritlizer containing willow leaf, including one time fermentation, secondary fermentation, specific steps are such as Under：
1) fresh willow leaf is collected；
2) by step 1) obtained material crushed；
3) one time fermentation：By step 2) obtained raw material packed with 3 layers of gauze, and making turns into willow leaf bag, and Add it in fermentation tank, have the following raw material according to mass percent in fermentation tank：
Willow leaf：65%；
Molasses：7%；
Urea：5%；
Probiotics strain：6%；
Water：Surplus；
4) according to step 3) ratio, the addition of each component is finished and starts ventilation and stirs, the requirement of ventilation is energy Seethe the liquid in fermentation tank, ventilation time is 25min；
5) after the completion of divulging information, fermentation tank is sealed, under the conditions of 35 DEG C, anaerobic fermentation 4 days；
6) after the completion of anaerobic fermentation, fermentation tank is opened, divulge information 40min, carries out aerobic fermentation, time 15 at normal temperatures afterwards My god；
7) monitoring raw material juice pH value change at any time, when pH is dropped to below 4, terminates aerobic fermentation；
8) by step 7) fermenting raw materials liquid carry out filtered 80 mesh sieve, obtain clear liquid, that is, obtain willow leaf mother liquor；
9) by step 8) obtained willow leaf mother liquor is transferred in bucket, and lucifuge room temperature preservation is standby；
10) secondary fermentation
According to parts by weight, each component composition is as follows：
Willow leaf mother liquor：75.5 parts；
Molasses：7 parts；
Urea：5 parts；
Wheat bran water：1.5 part；
Compound amino acid：0.5 part；
Composite trace element：0.2 part；
Potassium dihydrogen phosphate：2 parts；
Calcium dihydrogen phosphate：5 parts；
Mannitol：1.3 part；
Magnesium sulfate：2 parts；
11) above material is proportionally added in fermentation tank, fully dissolving, and stirring；
12) by material heat temperature raising, 40 DEG C is reached to temperature of charge, air is passed through, 30min is sufficiently stirred for；
13) after the completion of stirring, according to the 8% of material cumulative volume, probiotics strain is added, inoculation is completed, opened air and stir Mix, time 45min；
14) step 13) after the completion of, fermentation tank is sealed, temperature control is in 40-45 DEG C, anaerobic fermentation 24h；
15) step 14 is completed) after, fermentation tank is opened, 30min is stirred in ventilation, and temperature control is in 25-30 DEG C, aerobic fermentation 4-5 days, until one layer of white mycoderm occurs in zymotic fluid top layer；
16) occur after white mycoderm, zymotic fluid is poured out into bucket from fermentation tank, lucifuge room temperature preservation, no It is tight with the lid sealing bucket, a fixed gap is left, afterripening fermentation process is carried out；
17) detection pH changes, treat that pH is down to less than 4, afterripening fermentation is completed, and the liquid bacterial fertilizer of leaf containing willow completes；
The index of the bio-feritlizer containing willow leaf, wherein liquid bacterial manure that the present invention is obtained：Its content of beary metal Hg=0.13mg/kg, As=1.2mg/kg, Cd=0.33mg/kg, Pb=3.7mg/kg, Cr=6.9mg/kg；Living bacteria count =3.5 hundred million/ml, total nutrient=5.9%, micronutrient element=0.75%, the content of organic matter=143g/L；
It can be seen that the content of beary metal of above-mentioned ferment fertilizer is significantly lower than the scope of national Specification, meet wanting for product Ask, its living bacteria count respectively reaches 3.5 hundred million/ml, illustrate and the ferment fertilizer of the invention made surpasses in the content of microorganism Guo Liao China《Agricultural microbial agent》Standard, illustrate that the product just has obvious advantage on living bacteria count.Secondly, should Other indexs of product also completely within the scope of the related Water soluble fertilizer of national regulation, illustrate that the combination property of this product is accorded with Close the requirement of country.
Embodiment 2
A kind of preparation method of the bio-feritlizer containing willow leaf, including one time fermentation, secondary fermentation, specific steps are such as Under：
1) fresh willow leaf is collected；
2) by step 1) obtained material crushed；
3) one time fermentation：By step 2) obtained raw material packed with 3 layers of gauze, and making turns into willow leaf bag, and Add it in fermentation tank, have the following raw material according to mass percent in fermentation tank：
Willow leaf：60%；
Molasses：5%；
Urea：4%；
Probiotics strain：3%；
Water：Surplus；
4) according to step 3) ratio, the addition of each component is finished and starts ventilation and stirs, the requirement of ventilation is energy Seethe the liquid in fermentation tank, ventilation time is 20min；
5) after the completion of divulging information, fermentation tank is sealed, under the conditions of 38 DEG C, anaerobic fermentation 4 days；
6) after the completion of anaerobic fermentation, fermentation tank is opened, divulge information 50min, carries out aerobic fermentation, time 15 at normal temperatures afterwards My god；
7) monitoring raw material juice pH value change at any time, when pH is dropped to below 4, terminates aerobic fermentation；
8) by step 7) fermenting raw materials liquid carry out filtered 80 mesh sieve, obtain clear liquid, that is, obtain willow leaf mother liquor；
9) by step 8) obtained willow leaf mother liquor is transferred in bucket, and lucifuge room temperature preservation is standby；
10) secondary fermentation
According to parts by weight, each component composition is as follows：
Willow leaf mother liquor：73.5 parts；
Molasses：8 parts；
Urea：5 parts；
Wheat bran water：2 parts；
Compound amino acid：1 part；
Composite trace element：0.5 part；
Potassium dihydrogen phosphate：2 parts；
Calcium dihydrogen phosphate：5 parts；
Mannitol：1 part；
Magnesium sulfate：2 parts；
11) above material is proportionally added in fermentation tank, fully dissolving, and stirring；
12) by material heat temperature raising, 40 DEG C is reached to temperature of charge, air is passed through, 30min is sufficiently stirred for；
13) after the completion of stirring, according to the 5% of material cumulative volume, probiotics strain is added, inoculation is completed, opened air and stir Mix, time 45min；
14) step 13) after the completion of, fermentation tank is sealed, temperature control is in 45 DEG C, anaerobic fermentation 24h；
15) step 14 is completed) after, fermentation tank is opened, 30min is stirred in ventilation, and temperature control is in 25-30 DEG C, aerobic fermentation 4-5 days, until one layer of white mycoderm occurs in zymotic fluid top layer；
16) occur after white mycoderm, zymotic fluid is poured out into bucket from fermentation tank, lucifuge room temperature preservation, no It is tight with the lid sealing bucket, a fixed gap is left, afterripening fermentation process is carried out；
17) detection pH changes, treat that pH is down to less than 4, afterripening fermentation is completed, and the liquid bacterial fertilizer of leaf containing willow completes；
The index of the bio-feritlizer containing willow leaf, wherein liquid bacterial manure that the present invention is obtained：Its content of beary metal Hg=0.35mg/kg, As=1.15mg/kg, Cd=0.27mg/kg, Pb=3.00mg/kg, Cr=5.40mg/kg；It is effectively living Bacterium number=3.9 hundred million/ml, total nutrient=5.5%, micronutrient element=0.67%, the content of organic matter=170g/L.
Embodiment 3
A kind of preparation method of the bio-feritlizer containing willow leaf, including one time fermentation, secondary fermentation, specific steps are such as Under：
1) fresh willow leaf is collected；
2) by step 1) obtained material crushed；
3) one time fermentation：By step 2) obtained raw material packed with 3 layers of gauze, and making turns into willow leaf bag, and Add it in fermentation tank, have the following raw material according to mass percent in fermentation tank：
Willow leaf：55%；
Molasses：8%；
Urea：7%；
Probiotics strain：7%；
Water：Surplus；
4) according to step 3) ratio, the addition of each component is finished and starts ventilation and stirs, the requirement of ventilation is energy Seethe the liquid in fermentation tank, ventilation time is 20min；
5) after the completion of divulging information, fermentation tank is sealed, under the conditions of 40 DEG C, anaerobic fermentation 4 days；
6) after the completion of anaerobic fermentation, fermentation tank is opened, divulge information 60min, carries out aerobic fermentation, time 15 at normal temperatures afterwards My god；
7) monitoring raw material juice pH value change at any time, when pH is dropped to below 4, terminates aerobic fermentation；
8) by step 7) fermenting raw materials liquid carry out filtered 80 mesh sieve, obtain clear liquid, that is, obtain willow leaf mother liquor, filter Residue in slag and willow leaf bag merges into willow leaf slag；
9) by step 8) obtained willow leaf mother liquor is transferred in bucket, and lucifuge room temperature preservation is standby；
10) secondary fermentation
According to parts by weight, each component composition is as follows：
Willow leaf mother liquor：79 parts；
Molasses：6 parts；
Urea：5 parts；
Wheat bran water：1.5 part；
Compound amino acid：0.5 part；
Composite trace element：0.2 part；
Potassium dihydrogen phosphate：1 part；
Calcium dihydrogen phosphate：5 parts；
Mannitol：0.8 part；
Magnesium sulfate：1 part；
11) above material is proportionally added in fermentation tank, fully dissolving, and stirring；
12) by material heat temperature raising, 40 DEG C is reached to temperature of charge, air is passed through, 30min is sufficiently stirred for；
13) after the completion of stirring, according to the 10% of material cumulative volume, probiotics strain is added, inoculation is completed, opened air and stir Mix, time 45min；
14) step 13) after the completion of, fermentation tank is sealed, temperature control is in 40-45 DEG C, anaerobic fermentation 24h；
15) step 14 is completed) after, fermentation tank is opened, 30min is stirred in ventilation, and temperature control is in 25-30 DEG C, aerobic fermentation 4-5 days, until one layer of white mycoderm occurs in zymotic fluid top layer；
16) occur after white mycoderm, zymotic fluid is poured out into bucket from fermentation tank, lucifuge room temperature preservation, no It is tight with the lid sealing bucket, a fixed gap is left, afterripening fermentation process is carried out；
17) detection pH changes, treat that pH is down to less than 4, afterripening fermentation is completed, and the liquid bacterial fertilizer of leaf containing willow completes；
The index of the bio-feritlizer containing willow leaf, wherein liquid bacterial manure that the present invention is obtained：Its content of beary metal Hg=0.37mg/kg, As=1.54mg/kg, Cd=0.43mg/kg, Pb=3.09mg/kg, Cr=8.69mg/kg；It is effectively living Bacterium number=4.2 hundred million/ml, total nutrient=6.4%, micronutrient element=0.66%, the content of organic matter=177g/L.
Test example
The bio-feritlizer containing willow leaf obtained using embodiment 1 carries out field experiment, and control group is normal conventional Plantation spring stubble and autumn stubble tomato, as a result as follows in plantation, greenhouse：
Bio-feritlizer operational version (1.2 mu green house of vegetables) of the embodiment 1 containing willow leaf
Control group is planted for normal conventional, the liquid biological produced using Beijing Century Aims Biotechnology Co., Ltd Bacterial manure.
Yield comparison：
The incidence of disease is contrasted：
It can be seen that the bio-feritlizer produced by the invention containing willow leaf has, living bacteria count is high, nutrition is balanced, abundant, Action effect substantially, the features such as have a safety feature, can substantially increase fruits and vegetables yield, reduces the incidence of disease, while realizing a variety of The regeneration of discarded agricultural resource.

Claims (4)

1. a kind of preparation method of the bio-feritlizer containing willow leaf, including one time fermentation, secondary fermentation, specific steps are such as Under：
1) fresh willow, willow spray and tender leaf is collected；
2) by step 1) obtained material carries out mashing crushing；
3) one time fermentation：By step 2) obtained raw material packed with 3 layers of gauze, and making turns into willow leaf bag, and is added Enter into fermentation tank, have the following raw material according to mass percent in fermentation tank：
Willow leaf：50-70%；
Molasses：5-10%；
Urea：0.5-2%；
Probiotics strain：3-7%；
Water：Surplus；
4) according to step 3) ratio, the addition of each component is finished and starts ventilation and stirs, the requirement of ventilation is can make hair Liquid in fermentation tank is seethed, and ventilation time is 20-30min；
5) after the completion of divulging information, fermentation tank is sealed, under the conditions of 35-40 DEG C, anaerobic fermentation 4-5 days；
6) after the completion of anaerobic fermentation, fermentation tank is opened, divulge information 40-60min, carries out aerobic fermentation, time 15- at normal temperatures afterwards 20 days；
7) monitoring raw material juice pH value change at any time, when pH is dropped to below 4, terminates aerobic fermentation；
8) by step 7) fermenting raw materials liquid carry out filtered 80 mesh sieve, obtain clear liquid, that is, obtain willow leaf bacterial manure mother liquor；
9) by step 8) obtained willow leaf bacterial manure mother liquor is transferred in bucket, and lucifuge room temperature preservation is standby；
10) secondary fermentation
According to parts by weight, each component composition is as follows：
Willow leaf bacterial manure mother liquor：70-90 parts；
Molasses：5-8 parts；
Urea：3-8 parts；
Wheat bran water：1-2 parts；
Compound amino acid：0.5-1 parts；
Composite trace element：0.2-0.5 parts；
Potassium dihydrogen phosphate：1-2 parts；
Calcium dihydrogen phosphate：4-7 parts；
Magnesium sulfate：1-2 parts；
Mannitol：0.5-2 parts；
11) above material is proportionally added in fermentation tank, fully dissolving, and stirring；
12) by material heat temperature raising, 40-45 DEG C is reached to temperature of charge, air is passed through, 30min is sufficiently stirred for；
13) after the completion of stirring, according to the 5-10% of material cumulative volume, probiotics strain is added, inoculation is completed, opened air and stir Mix, time 30-60min；
14) step 13) after the completion of, fermentation tank is sealed, temperature control is in 40-45 DEG C, anaerobic fermentation 24-36h；
15) step 14 is completed) after, fermentation tank is opened, 30min is stirred in ventilation, and temperature control is in 25-30 DEG C, aerobic fermentation 4-5 My god, until one layer of white mycoderm occurs in zymotic fluid top layer；
16) occur after white mycoderm, zymotic fluid is poured out into bucket from fermentation tank, lucifuge room temperature preservation, without handle The lid sealing of bucket is tight, leaves a fixed gap, carries out afterripening fermentation process；
17) detection pH changes, treat that pH is down to less than 4, afterripening fermentation is completed, and the bio-feritlizer containing willow leaf completes.
2. the preparation method of the bio-feritlizer according to claim 1 containing willow leaf, it is characterised in that：Described benefit Raw bacterium strain is selected from probiotics liquid spawn, specially moistens Kang Yuan EM stostes purchased from Sino-Japan joint Linyi Yikang organic agriculture science and technology Garden Co., Ltd.
3. the preparation method of the bio-feritlizer according to claim 1 containing willow leaf, it is characterised in that：By quality point Number meter, described compound amino acid active ingredient >=98%；Composite trace element active ingredient >=98%；Potassium dihydrogen phosphate is effective Composition >=80%；Calcium dihydrogen phosphate active ingredient >=80%；Mannitol active ingredient >=98%；Magnesium sulfate active ingredient >= 80%.
4. the preparation method of the bio-feritlizer according to claim 1 containing willow leaf, it is characterised in that：The step 10) the wheat bran water in is：Wheat bran is soaked according to the warm water of 5 times of wheat bran volumes 5-6 hours, takes supernatant.
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106866285A (en) * 2017-04-11 2017-06-20 山东博华高效生态农业科技有限公司 A kind of preparation method of the bio-feritlizer containing ginkgo leaf
CN110419542A (en) * 2019-07-22 2019-11-08 湖州盛明生物科技有限公司 A kind of Plant growth-promoting effect element and preparation method thereof
CN110892842A (en) * 2019-12-02 2020-03-20 务川自治县花园益农种养农民专业合作社 Planting method of pepper

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CN104276895A (en) * 2014-10-24 2015-01-14 国家粳稻工程技术研究中心 Green ecological medicinal fertilizer for vegetables and preparation method of green ecological medicinal fertilizer
CN104529573A (en) * 2014-12-30 2015-04-22 北海万物盛生物技术开发有限公司 Multi-effect ferment fertilizer prepared from agricultural wastes
CN106235306A (en) * 2016-07-27 2016-12-21 杜增鹏 Liquid fermentation method biological fruit and vegetable ferment and production method thereof and purposes

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CN102887741A (en) * 2012-10-31 2013-01-23 青岛嘉禾丰肥业有限公司 Organic fertilizer prepared by fermenting straws
CN104276895A (en) * 2014-10-24 2015-01-14 国家粳稻工程技术研究中心 Green ecological medicinal fertilizer for vegetables and preparation method of green ecological medicinal fertilizer
CN104529573A (en) * 2014-12-30 2015-04-22 北海万物盛生物技术开发有限公司 Multi-effect ferment fertilizer prepared from agricultural wastes
CN106235306A (en) * 2016-07-27 2016-12-21 杜增鹏 Liquid fermentation method biological fruit and vegetable ferment and production method thereof and purposes

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106866285A (en) * 2017-04-11 2017-06-20 山东博华高效生态农业科技有限公司 A kind of preparation method of the bio-feritlizer containing ginkgo leaf
CN110419542A (en) * 2019-07-22 2019-11-08 湖州盛明生物科技有限公司 A kind of Plant growth-promoting effect element and preparation method thereof
CN110892842A (en) * 2019-12-02 2020-03-20 务川自治县花园益农种养农民专业合作社 Planting method of pepper

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Application publication date: 20170818