CN107046973A - A kind of high yield, anti-fall cold spell in spring, the breeding method of anti-disease wheat new varieties - Google Patents

A kind of high yield, anti-fall cold spell in spring, the breeding method of anti-disease wheat new varieties Download PDF

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CN107046973A
CN107046973A CN201710037442.7A CN201710037442A CN107046973A CN 107046973 A CN107046973 A CN 107046973A CN 201710037442 A CN201710037442 A CN 201710037442A CN 107046973 A CN107046973 A CN 107046973A
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wheat
spring
seed
spring tea
blade
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王宗启
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Anhui Province Dongchang Agricultural Science And Technology Co Ltd
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Anhui Province Dongchang Agricultural Science And Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A01H3/04Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Biodiversity & Conservation Biology (AREA)
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  • Forests & Forestry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Cultivation Of Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a kind of high yield, anti-fall cold spell in spring, anti-disease wheat new varieties breeding method, step is as follows:(1)Wheat, which breaks to be put into 0~3 degree of low temperature bud case after dormancy vernalization, carries out pressure vernalization, high 3 centimeters;(2)Cultivated with spring tea blade;(3)Intelligent biotron children plants, and cut off at tillering node wheat with sterilization blade, reservation wheat tillering section top is allocated as scion;(4)Carried out getting out grafting hole with spring tea blade, grafting hole and surrounding are sterilized;(5)By in scion insertion grafting hole;(6):In the nutrition incubator for moving into sterilization treatment;(7)Artificial intelligence climatic chamber is moved into, wheat seed is harvested;(8)By wheat seed as first filial generation seed, after plantation is selected, second filial seed is obtained;(9)After the plantation of second filial seed, F3 seed is obtained.The present invention improves the yield of new varieties wheat, improves the anti-fall cold spell in spring of wheat, the Optimality such as disease-resistant, and then adds income, and satisfaction is actually needed.

Description

A kind of high yield, anti-fall cold spell in spring, the breeding method of anti-disease wheat new varieties
Technical field
The present invention relates to a kind of high yield, anti-fall cold spell in spring, anti-disease wheat new varieties breeding method, belong to new variety of wheat training Educate method and technology field.
Background technology
Wheat is one of three big cereal, is a kind of staple food crop widely planted in the world, yield almost full conduct It is edible.Wheat is the general designation of wheat system plant, is monocotyledon, is that a kind of grass family planted extensively all over the world is planted Thing, the caryopsis of wheat is one of staple food of the mankind, and the foods such as bread, steamed bun, biscuit, noodles can be made by wearing into after flour;Fermentation After can be made into beer, alcohol, white wine(Such as vodka), or raw matter fuel.Wheat rich in starch, protein, fat, mineral matter, Calcium, iron, thiamine, riboflavin, nicotinic acid, vitamin A and vitamin C etc..Wheat is one of three big cereal, is almost eaten entirely, 1/6th are only there are about to use as feed.The Yellow Basin and the Yangtze River is the area of earliest cultivated wheat in the world, and China is that the world is earliest Plant one of country of wheat.Wheat in 2010 is the cereal crops (6.51 hundred million tons) that total output occupies second in the world, only secondary In corn (8.44 hundred million tons).
At present:New variety of wheat is without the good characteristic such as high yield, anti-fall cold spell in spring, disease-resistant, in addition, often due to existing skill Art is on the incorrect of breeding method, management is incorrect and causes the new varieties wheat seed quality dissatisfaction of cultivation, so may be used Unnecessary economic loss can just be brought.For this reason, it may be necessary to improve a kind of new breeding method, comprehensive it can overcome above-mentioned existing With the presence of the deficiency of technology.
The content of the invention
The present invention is exactly in view of the deficienciess of the prior art, provide a kind of high yield, anti-fall cold spell in spring, anti-disease wheat new varieties Breeding method, this method change conventional wheat breeding method, improve the yield of new varieties wheat, improve new varieties small The Optimality such as the anti-fall anti-cold spell in spring of wheat, disease-resistant, and then income is added, satisfaction is actually needed.
To solve the above problems, the technical solution used in the present invention is as follows:
A kind of high yield, anti-fall cold spell in spring, the breeding method of anti-disease wheat new varieties, the breeding method step are as follows:
Step(1):Wheat, which breaks to be put into 0~3 degree of low temperature bud case after dormancy vernalization, carries out pressure vernalization, completes small after 36~40 days The pressure vernalization of wheat, now wheat is high 3 centimeters in seedling stage;
Step(2):Spring tea is planted, when spring tea leaf growth is to 60~80mm, from root cut-out;
Step(3):Wheat seedling, spring tea blade are moved in calm sterile intelligent biotron, in intelligent biotron Control temperature be maintained at 28~32 degree between, humidity be 40~60%, wheat cut off at tillering node with sterilization blade, reservation Wheat tillering section top is allocated as scion, and the cut-off part of wheat is sterilized;
Step(4):Stock is done with spring tea blade, on 15~25mm positions, is being bored on spring tea root of blade with 1.2mm drill bit Go out grafting hole, grafting hole depth is the 1/3~1/2 of spring tea vane thickness, and grafting hole and surrounding position are sterilized;
Step(5):By in scion insertion grafting hole, will together it be tightly wrapped as the spring tea blade of stock and scion with medical proof fabric;
Step(6):In the nutrition incubator for moving into sterilization treatment, the spring tea blade of scion is planted in Nutrition Soil, Nutrition Soil Covering is to more than grafting hole 4~8mm, and nutrition incubator black out, temperature is 22~26 degree, is cultivated 22~28 days;
When having just enter into nutrition incubator, atomizing spray, the water of spray in every 1~3 hour, until wheat is long are carried out to the spring tea of scion When going out first leaf, stop water spray;
The nutrient solution being made into nutrient, auxin and the basic element of cell division is applied fertilizer, and is sprayed week about once, spray concentration For 200ppm;
Step(7):Artificial intelligence climatic chamber is moved into, wheat late growing stage phase condition is simulated, indoor temperature is 26~32 degree, humidity For 60~80%, daytime natural light, evening is ripe with wheat after simulated light 7~11 hours, 80 days, can harvest Wheat Species Son;
Step(8):Wheat seed is used for field planting as first filial generation seed, 50%, the plant of variation, 50% is therefrom obtained As seed, grafting backcrossing is carried out with spring tea blade again, second filial seed is obtained;
Step(9):It is used for field planting by the 50% of second filial seed, therefrom obtains the plant of variation, 50% as seed, the Three times and the progress grafting backcrossing of spring tea blade, obtain F3 seed.
As the improvement of above-mentioned technical proposal, in step 3 and step 4, the solution carried out disinfection to spring tea blade, scion For absolute alcohol, its concentration is 94%.
As the improvement of above-mentioned technical proposal, in step 6, described nutrient solution is nutrient, auxin and cell point Split element to be uniformly mixed, its mass ratio is 1:1:1.
Compared with prior art, implementation result of the invention is as follows by the present invention:
A kind of high yield of the present invention, anti-fall cold spell in spring, the breeding method of anti-disease wheat new varieties, be according to principle, it is biological apparent Science of heredity is thought:Any one cell with certain biological whole hereditary information has the potential for developing into intact organism, That is, the characteristics of each biological cell has totipotency(Recuperation of the spring tea Jing Guo a Winter-Spring, nutrient accumulation, nutrient fills Foot, tealeaves inclusion is often most abundant;Temperature is relatively low, is conducive to the synthesis of nitrogen-containing compound in accumulation, free ammonia The nutrient composition contents such as base acid, protein are higher, and pest and disease damage generation is fewer, spraying pesticide is not needed typically, so spring tea one As without pollution by pesticides;And the catechin that spring tea contains has anti-oxidant, anti-mutation mutation, antitumor, reduction Blood Cholesterol And the effects such as low-density lipoprotein content, the rising of suppression blood pressure, suppression platelet aggregation, antibacterial, anti-product allergy).Therefore, exist In theory, any one biological cell all has the potentiality for developing into intact plant.The present invention high yield, the anti-spring fall cold-resistant, The breeding method of anti-disease wheat new varieties is analog resistant mutant, and asexual hybridization does not change gene order, but gene in breeding process Expression but there occurs heritable change, and this change energy stable delivery during development and cell proliferation, so as to obtain High yield, anti-fall cold spell in spring, anti-disease wheat offspring's new lines.General breeding method needs the new product that 8-10 can just be cultivated Kind, and the breeding method of the present invention shorten to triennium, just can cultivate a new varieties, so as to shorten breeding time, carry Height cultivates new varieties speed.
Embodiment
Illustrate present disclosure below in conjunction with specific embodiments.
Embodiment 1
A kind of high yield, anti-fall cold spell in spring, the breeding method of anti-disease wheat new varieties, the breeding method step are as follows:
Step(1):Wheat, which breaks to be put into 0 degree of low temperature bud case after dormancy vernalization, carries out pressure vernalization, and the strong of wheat is completed after 36 days Vernalization processed, now wheat is high 3 centimeters in seedling stage;
Step(2):Spring tea is planted, when spring tea leaf growth is to 60mm, from root cut-out;
Step(3):Wheat seedling, spring tea blade are moved in calm sterile intelligent biotron, in intelligent biotron Control temperature be maintained at 28 degree between, humidity be 40%, wheat cut off at tillering node with sterilization blade, reservation wheat tillering Section top is allocated as scion, and the cut-off part of wheat is sterilized;
Step(4):Stock is done with spring tea blade, on 15mm positions, is being got out and transferred with 1.2mm drill bit on spring tea root of blade Hole is connect, grafting hole depth is the 1/3 of spring tea vane thickness, and grafting hole and surrounding position are sterilized;
Step(5):By in scion insertion grafting hole, will together it be tightly wrapped as the spring tea blade of stock and scion with medical proof fabric;
Step(6):In the nutrition incubator for moving into sterilization treatment, the spring tea blade of scion is planted in Nutrition Soil, Nutrition Soil Covering is to grafting hole above 4mm, and nutrition incubator black out, temperature is 22 degree, is cultivated 22 days;
When having just enter into nutrition incubator, atomizing spray, the water of spray in every 1 hour, until wheat is grown are carried out to the spring tea of scion During first leaf, stop water spray;
The nutrient solution being made into nutrient, auxin and the basic element of cell division is applied fertilizer, and is sprayed week about once, spray concentration For 200ppm;
Step(7):Artificial intelligence climatic chamber is moved into, wheat late growing stage phase condition is simulated, indoor temperature is 26 degree, humidity is 60%, daytime natural light, evening is ripe with wheat after simulated light 7 hours, 80 days, can harvest wheat seed;
Step(8):Wheat seed is used for field planting as first filial generation seed, 50%, the plant of variation, 50% is therefrom obtained As seed, grafting backcrossing is carried out with spring tea blade again, second filial seed is obtained;
Step(9):It is used for field planting by the 50% of second filial seed, therefrom obtains the plant of variation, 50% as seed, the Three times and the progress grafting backcrossing of spring tea blade, obtain F3 seed.
More specifically, in step 3 and step 4, the solution carried out disinfection to spring tea blade, scion is absolute alcohol, its Concentration is 94%;In step 6, described nutrient solution is that nutrient, auxin and the basic element of cell division are uniformly mixed, its Mass ratio is 1:1:1.
The present invention is that biological epigenetics thinks according to principle:Any one with certain biological whole hereditary information Individual cell has the potential for developing into intact organism, that is to say, that the characteristics of each biological cell has totipotency(Spring tea By the recuperation of a Winter-Spring, nutrient accumulation, nutrient is sufficient, and tealeaves inclusion is often most abundant;Temperature is relatively low, favorably In nitrogen-containing compound synthesis in accumulation, the nutrient composition content such as free amino acid, protein is higher, and pest and disease damage compares It is few, spraying pesticide is not needed typically, so spring tea is general without pollution by pesticides;And the catechin tool that spring tea contains is anti-oxidant, anti- Mutation mutation, antitumor, reduction Blood Cholesterol and low-density lipoprotein content, suppression blood pressure rise, suppression blood platelet is solidifying The effects such as poly-, antibacterial, anti-product allergy).Therefore, in theory, any one biological cell, which all has, develops into complete plant The potentiality of strain.The present invention high yield, the anti-spring fall cold-resistant, anti-disease wheat new varieties breeding method be analog resistant mutant, asexual hybridization, Do not change gene order in breeding process, but gene expression there occurs heritable change, and it is this change in development and Energy stable delivery during cell proliferation, so as to obtain high yield, anti-fall cold spell in spring, anti-disease wheat offspring's new lines.General cultivation Method needs the new varieties that 8-10 can just be cultivated, and the breeding method of the present invention shorten to triennium, can just train A new varieties are brought out, so as to shorten breeding time, improves and cultivates new varieties speed.
Embodiment 2
A kind of high yield, anti-fall cold spell in spring, the breeding method of anti-disease wheat new varieties, the breeding method step are as follows:
Step(1):Wheat, which breaks to be put into 1~2 degree of low temperature bud case after dormancy vernalization, carries out pressure vernalization, completes small after 36~40 days The pressure vernalization of wheat, now wheat is high 3 centimeters in seedling stage;
Step(2):Spring tea is planted, when spring tea leaf growth is to 70mm, from root cut-out;
Step(3):Wheat seedling, spring tea blade are moved in calm sterile intelligent biotron, in intelligent biotron Control temperature be maintained at 30 degree between, humidity be 50%, wheat cut off at tillering node with sterilization blade, reservation wheat tillering Section top is allocated as scion, and the cut-off part of wheat is sterilized;
Step(4):Stock is done with spring tea blade, on 20mm positions, is being got out and transferred with 1.2mm drill bit on spring tea root of blade Hole is connect, grafting hole depth is the 5/12 of spring tea vane thickness, and grafting hole and surrounding position are sterilized;
Step(5):By in scion insertion grafting hole, will together it be tightly wrapped as the spring tea blade of stock and scion with medical proof fabric;
Step(6):In the nutrition incubator for moving into sterilization treatment, the spring tea blade of scion is planted in Nutrition Soil, Nutrition Soil Covering is to grafting hole above 6mm, and nutrition incubator black out, temperature is 24 degree, is cultivated 25 days;
When having just enter into nutrition incubator, atomizing spray, the water of spray in every 2 hours, until wheat is grown are carried out to the spring tea of scion During first leaf, stop water spray;
The nutrient solution being made into nutrient, auxin and the basic element of cell division is applied fertilizer, and is sprayed week about once, spray concentration For 200ppm;
Step(7):Artificial intelligence climatic chamber is moved into, wheat late growing stage phase condition is simulated, indoor temperature is 29 degree, humidity is 70%, daytime natural light, evening is ripe with wheat after simulated light 9 hours, 80 days, can harvest wheat seed;
Step(8):Wheat seed is used for field planting as first filial generation seed, 50%, the plant of variation, 50% is therefrom obtained As seed, grafting backcrossing is carried out with spring tea blade again, second filial seed is obtained;
Step(9):It is used for field planting by the 50% of second filial seed, therefrom obtains the plant of variation, 50% as seed, the Three times and the progress grafting backcrossing of spring tea blade, obtain F3 seed.
More specifically, in step 3 and step 4, the solution carried out disinfection to spring tea blade, scion is absolute alcohol, its Concentration is 94%;In step 6, described nutrient solution is that nutrient, auxin and the basic element of cell division are uniformly mixed, its Mass ratio is 1:1:1.
The present invention is that biological epigenetics thinks according to principle:Any one with certain biological whole hereditary information Individual cell has the potential for developing into intact organism, that is to say, that the characteristics of each biological cell has totipotency(Spring tea By the recuperation of a Winter-Spring, nutrient accumulation, nutrient is sufficient, and tealeaves inclusion is often most abundant;Temperature is relatively low, favorably In nitrogen-containing compound synthesis in accumulation, the nutrient composition content such as free amino acid, protein is higher, and pest and disease damage compares It is few, spraying pesticide is not needed typically, so spring tea is general without pollution by pesticides;And the catechin tool that spring tea contains is anti-oxidant, anti- Mutation mutation, antitumor, reduction Blood Cholesterol and low-density lipoprotein content, suppression blood pressure rise, suppression blood platelet is solidifying The effects such as poly-, antibacterial, anti-product allergy).Therefore, in theory, any one biological cell, which all has, develops into complete plant The potentiality of strain.The present invention high yield, the anti-spring fall cold-resistant, anti-disease wheat new varieties breeding method be analog resistant mutant, asexual hybridization, Do not change gene order in breeding process, but gene expression there occurs heritable change, and it is this change in development and Energy stable delivery during cell proliferation, so as to obtain high yield, anti-fall cold spell in spring, anti-disease wheat offspring's new lines.General cultivation Method needs the new varieties that 8-10 can just be cultivated, and the breeding method of the present invention shorten to triennium, can just train A new varieties are brought out, so as to shorten breeding time, improves and cultivates new varieties speed.
Embodiment 3
A kind of high yield, anti-fall cold spell in spring, the breeding method of anti-disease wheat new varieties, the breeding method step are as follows:
Step(1):Wheat, which breaks to be put into 3 degree of low temperature bud case after dormancy vernalization, carries out pressure vernalization, and the strong of wheat is completed after 40 days Vernalization processed, now wheat is high 3 centimeters in seedling stage;
Step(2):Spring tea is planted, when spring tea leaf growth is to 80mm, from root cut-out;
Step(3):Wheat seedling, spring tea blade are moved in calm sterile intelligent biotron, in intelligent biotron Control temperature be maintained at 32 degree between, humidity be 60%, wheat cut off at tillering node with sterilization blade, reservation wheat tillering Section top is allocated as scion, and the cut-off part of wheat is sterilized;
Step(4):Stock is done with spring tea blade, on 25mm positions, is being got out and transferred with 1.2mm drill bit on spring tea root of blade Hole is connect, grafting hole depth is the 1/2 of spring tea vane thickness, and grafting hole and surrounding position are sterilized;
Step(5):By in scion insertion grafting hole, will together it be tightly wrapped as the spring tea blade of stock and scion with medical proof fabric;
Step(6):In the nutrition incubator for moving into sterilization treatment, the spring tea blade of scion is planted in Nutrition Soil, Nutrition Soil Covering is to grafting hole above 8mm, and nutrition incubator black out, temperature is 26 degree, is cultivated 28 days;
When having just enter into nutrition incubator, atomizing spray, the water of spray in every 3 hours, until wheat is grown are carried out to the spring tea of scion During first leaf, stop water spray;
The nutrient solution being made into nutrient, auxin and the basic element of cell division is applied fertilizer, and is sprayed week about once, spray concentration For 200ppm;
Step(7):Artificial intelligence climatic chamber is moved into, wheat late growing stage phase condition is simulated, indoor temperature is 32 degree, humidity is 80%, daytime natural light, evening is ripe with wheat after simulated light 11 hours, 80 days, can harvest wheat seed;
Step(8):Wheat seed is used for field planting as first filial generation seed, 50%, the plant of variation, 50% is therefrom obtained As seed, grafting backcrossing is carried out with spring tea blade again, second filial seed is obtained;
Step(9):It is used for field planting by the 50% of second filial seed, therefrom obtains the plant of variation, 50% as seed, the Three times and the progress grafting backcrossing of spring tea blade, obtain F3 seed.
More specifically, in step 3 and step 4, the solution carried out disinfection to spring tea blade, scion is absolute alcohol, its Concentration is 94%;In step 6, described nutrient solution is that nutrient, auxin and the basic element of cell division are uniformly mixed, its Mass ratio is 1:1:1.
The present invention is that biological epigenetics thinks according to principle:Any one with certain biological whole hereditary information Individual cell has the potential for developing into intact organism, that is to say, that the characteristics of each biological cell has totipotency(Spring tea By the recuperation of a Winter-Spring, nutrient accumulation, nutrient is sufficient, and tealeaves inclusion is often most abundant;Temperature is relatively low, favorably In nitrogen-containing compound synthesis in accumulation, the nutrient composition content such as free amino acid, protein is higher, and pest and disease damage compares It is few, spraying pesticide is not needed typically, so spring tea is general without pollution by pesticides;And the catechin tool that spring tea contains is anti-oxidant, anti- Mutation mutation, antitumor, reduction Blood Cholesterol and low-density lipoprotein content, suppression blood pressure rise, suppression blood platelet is solidifying The effects such as poly-, antibacterial, anti-product allergy).Therefore, in theory, any one biological cell, which all has, develops into complete plant The potentiality of strain.The present invention high yield, the anti-spring fall cold-resistant, anti-disease wheat new varieties breeding method be analog resistant mutant, asexual hybridization, Do not change gene order in breeding process, but gene expression there occurs heritable change, and it is this change in development and Energy stable delivery during cell proliferation, so as to obtain high yield, anti-fall cold spell in spring, anti-disease wheat offspring's new lines.General cultivation Method needs the new varieties that 8-10 can just be cultivated, and the breeding method of the present invention shorten to triennium, can just train A new varieties are brought out, so as to shorten breeding time, improves and cultivates new varieties speed.
Note:Design parameter span can be according to actual conditions in each step in cultural method of the present invention(Can be according to tool The yield of the wheat reached needed for body, disease-resistant, anti-fall cold spell in spring performance etc.)Setting.
Above content is made for the present invention in conjunction with specific embodiments describes in detail, it is impossible to assert that the present invention is specific real Apply and be only limitted to these explanations.For those skilled in the art, before present inventive concept is not departed from Put, some simple deduction or replace can also be made, the scope of protection of the invention should be all considered as belonging to.

Claims (3)

1. a kind of high yield, anti-fall cold spell in spring, the breeding method of anti-disease wheat new varieties, it is characterised in that:The breeding method step is such as Under:
Step(1):Wheat, which breaks to be put into 0~3 degree of low temperature bud case after dormancy vernalization, carries out pressure vernalization, completes small after 36~40 days The pressure vernalization of wheat, now wheat is high 3 centimeters in seedling stage;
Step(2):Spring tea is planted, when spring tea leaf growth is to 60~80mm, from root cut-out;
Step(3):Wheat seedling, spring tea blade are moved in calm sterile intelligent biotron, in intelligent biotron Control temperature be maintained at 28~32 degree between, humidity be 40~60%, wheat cut off at tillering node with sterilization blade, reservation Wheat tillering section top is allocated as scion, and the cut-off part of wheat is sterilized;
Step(4):Stock is done with spring tea blade, on 15~25mm positions, is being bored on spring tea root of blade with 1.2mm drill bit Go out grafting hole, grafting hole depth is the 1/3~1/2 of spring tea vane thickness, and grafting hole and surrounding position are sterilized;
Step(5):By in scion insertion grafting hole, will together it be tightly wrapped as the spring tea blade of stock and scion with medical proof fabric;
Step(6):In the nutrition incubator for moving into sterilization treatment, the spring tea blade of scion is planted in Nutrition Soil, Nutrition Soil Covering is to more than grafting hole 4~8mm, and nutrition incubator black out, temperature is 22~26 degree, is cultivated 22~28 days;
When having just enter into nutrition incubator, atomizing spray, the water of spray in every 1~3 hour, until wheat is long are carried out to the spring tea of scion When going out first leaf, stop water spray;
The nutrient solution being made into nutrient, auxin and the basic element of cell division is applied fertilizer, and is sprayed week about once, spray concentration For 200ppm;
Step(7):Artificial intelligence climatic chamber is moved into, wheat late growing stage phase condition is simulated, indoor temperature is 26~32 degree, humidity For 60~80%, daytime natural light, evening is ripe with wheat after simulated light 7~11 hours, 80 days, can harvest Wheat Species Son;
Step(8):Wheat seed is used for field planting as first filial generation seed, 50%, the plant of variation, 50% is therefrom obtained As seed, grafting backcrossing is carried out with spring tea blade again, second filial seed is obtained;
Step(9):It is used for field planting by the 50% of second filial seed, therefrom obtains the plant of variation, 50% as seed, the Three times and the progress grafting backcrossing of spring tea blade, obtain F3 seed.
2. a kind of high yield, anti-fall cold spell in spring, the breeding method of anti-disease wheat new varieties according to claim 1, it is characterised in that: In step 3 and step 4, the solution carried out disinfection to spring tea blade, scion is absolute alcohol, and its concentration is 94%.
3. a kind of high yield, anti-fall cold spell in spring, the breeding method of anti-disease wheat new varieties according to claim 1, it is characterised in that: In step 6, described nutrient solution is that nutrient, auxin and the basic element of cell division are uniformly mixed, and its mass ratio is 1:1: 1。
CN201710037442.7A 2017-01-19 2017-01-19 A kind of high yield, anti-fall cold spell in spring, the breeding method of anti-disease wheat new varieties Pending CN107046973A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110352724A (en) * 2019-08-15 2019-10-22 延安市技术转移中心 A kind of overwintering Juncao new varieties method for culturing seedlings

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104170644A (en) * 2014-08-31 2014-12-03 曹洪青 Method for cultivating new species of super-high-yield wheat

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104170644A (en) * 2014-08-31 2014-12-03 曹洪青 Method for cultivating new species of super-high-yield wheat

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110352724A (en) * 2019-08-15 2019-10-22 延安市技术转移中心 A kind of overwintering Juncao new varieties method for culturing seedlings

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