A kind of breeding method of new this squash hybridization of sweetness seed
Technical field
The present invention relates to the biotechnology breeding field, be specifically related to a kind of breeding method of new this squash hybridization of sweetness seed.
Background technology
Pumpkin is annual dicotyledonous herbaceous plant, originates in Southern Asia, and cultivation is all arranged all over the world, is the plant of Curcurbitaceae Cucurbita, can climb climingly, and edible is partly 85%; Energy content 92kJ in every 100g, moisture 93.5g, protein 0.7g, dietary fiber 0.8g, carotin 890 μ g, vitamin C 4mg, potassium 763mg, calcium 191mg, magnesium 47mg, selenium 0.3 μ g, Alphalin, vitamin B1, B2, F etc.
Pumpkin pulp is thick, crocus, special aroma is arranged after aging, the sweet and face of distinguishing the flavor of.Seed is flat, plants skin canescence or dark brown, and edge is obviously coarse, and tender melon can be done vegetables, is one of the main fruits and vegetables of summer and autumn on the south the Yangtze river basin, distinguishes the flavor of sweet agreeable to the taste, and old melon is cooked or, for sugar, seed can be stir-fried and eaten; Pumpkin, not only when dish but also for grain, also has certain food therapy value, and therefore the pure pumpkin of soil flavor is climbed up refined taste, deeply is subject to liking of town and country consumers in general.In the past in existing pumpkin variety, yielding ability and disease resistance are strong not, and quality is not high, there is the responsive various shortcoming of ripe, counterglow late, there are significant limitation plantation region or time, and long-term kind unification also is unfavorable for the long term growth of pumpkin industry, therefore pass through breeding of new variety, bring out precocity, high yield, high-quality, disease-resistant, the adaptable pumpkin new varieties of an applicable market demand, the development vegetables are produced, the satisfying the market supply, increase economic results in society, promotes the development of China's pumpkin industrialization that very important meaning is arranged.
Summary of the invention
The invention provides a kind of breeding method of new this squash hybridization of sweetness seed, pumpkin variety that the method obtains has that more common pumpkin variety output is high, ripe fast, cold resistance is strong, good heat resistance, strong adaptability and the advantages such as the pumpkin enduring store property is good that obtain.
The breeding method of squash hybridization seed of the present invention, concrete grammar is: take inbred line 04-9 as male parent, take inbred line 06-1 as maternal, mix plantation and hybridized, collect produced squash hybridization seed and get final product.
Described mixing in plantation, described male parent field planting is at two ends, and male parent and maternal spacing in the rows are 0.5m, and male parent is capable of being 1:8 with female parent.
Described male parent inbred line 04-9, its preparation method is: will grow the selfing of curcumbitate pumpkin material bagging, the offspring divides the plantation of 18 strains, directedly selects the individual plant material that melon is straight, the melon stem is larger, through 6 generation individual plant selection, obtain stable and the consistent good inbred line of curcumbitate.Described long curcumbitate pumpkin material is from the farm variety in Feng Tang town, Chaozhou, Guangdong.
The principal character of described male parent inbred line 04-9 is: plant overgrows, and growth is surging, and branchiness is strong, main climing green, climing thick approximately 1.2 centimetres, long 7-10 rice, leaf edge sawtooth shape, dark green leaf color, blade is substantially rounded, several 39 of single-strain blade, petal yellow, the tip acute angle, first female flower joint position is 19 joints approximately, and female flower is first opened, be separated by and within 2-3 days, open male flower again, main, lateral bine is all tied melon, in ripe, sowing, to gathering about 99-110 days, continues and to gather 15 days, the time of infertility 110-125 days.Melon long-neck cylindrical shape, the blackish green colour band an IOU issued by a post office of tender melon spot, ripe rear melon skin look pale brown band an IOU issued by a post office spot, approximately 1.6 of individual plant knot melon numbers, heavily approximately 3.5 kilograms, single melon; Yellowish pink orange, quality better.The growth field virus disease incidence of disease in mid-term approximately 3%, cold resistance is strong, and warm tolerance is better, anti-storage.
Described maternal inbred line 06-1, its preparation method is: the little fragrant honey of the conventional pumpkin kind of high-quality is hybridized with drawing from the early pumpkin material, then the bagging selfing separates, in progeny material, continuous multi-generation is selected the fine individual plant of high-quality, precocity, long oval curcumbitate, during to the 8th generation, Other Main Agronomic Characters is stable; From this, stable strain, select a fine individual plant as maternal 05-1, with described inbred line 04-9 hybridization; The next generation backcrosses with maternal 05-1, and its offspring's continuous bag sleeving selfing separates, and orientation is selected precocity, high-quality, disease-resistant, long oval curcumbitate and the individual large individual plant material of melon, selects the good inbred line 06-1 of main stabilization characteristics of genetics through 12 generations.Described early pumpkin material is from the farm variety in bayhead town, Shantou, Guangdong city.
The principal character of described maternal inbred line 06-1 is: plant overgrows, and growth potential and branchiness are strong, main climing green, and thick approximately 1.1 centimetres, long 8 meters left and right, leaf edge sawtooth shape, dark green leaf color, it is circular that blade belongs to substantially, several 39 of single-strain blade, petal yellow, tip acute angle; First female flower joint position is 15 joints approximately, and female flower is first opened, and after three, four days, opens male flower, and the female florescence concentrates, precocious, and sowing is to the 93-100 days that gathers, approximately 10 days picking time of continuity, the time of infertility 103-110 days.Female flower is many, and main, lateral bine is all tied melon, and the seat melon is good, approximately 2.3, individual plant knot melon; The melon oblong, grey greenbelt brown color striped, single melon weighs 3.0 kilograms of left and right, yellowish pink orange, and quality better is rich in sugar.The growth field virus disease incidence of disease in mid-term approximately 5%, cold resistance is strong, and warm tolerance is better, storage endurance.
The squash hybridization seed that breeding method of the present invention obtains, it has the following advantages:
1. it cultivates plant cold resistance by force and good heat resistance;
Its to cultivate plant adaptability stronger, the pumpkin producing region spring on the south the Huanghe valley, Qiu Junke plantation, the place plantation in more past north, precocious characteristic is more obvious.
Its pumpkin product that obtains of the plant of cultivating, average single melon weight is more than 3.0kg, closeer pumpkin volume increase is more than 19%, than close early the receipts approximately more than five days of pumpkin.
Its pumpkin product that obtains of the plant of cultivating, storage property is better, the good and undamaged melon of the maturity place, rear normal temperature cool place that gathers can store more than 50 days.
Embodiment
Following specific embodiment, be in order further to set forth content of the present invention, and be not to be construed as limiting the scope of the invention.
embodiment 1
Male parent is cultivated: will grow the selfing of curcumbitate pumpkin material bagging, the offspring divides 18 strains plantations, directedly selects the individual plant material that melon is straight, the melon stem is larger, through 6 generation individual plant selection, obtain stable and the consistent good inbred line 04-9 of curcumbitate.
Female parent is cultivated: the little fragrant honey of the conventional pumpkin kind of high-quality is hybridized with drawing from the early pumpkin material, then the bagging selfing separates, in progeny material, continuous multi-generation is selected the fine individual plant of high-quality, precocity, long oval curcumbitate, and during to the 8th generation, Other Main Agronomic Characters is stable; From this, stable strain, select a fine individual plant as maternal 05-1, with described inbred line 04-9 hybridization; The next generation backcrosses with maternal 05-1, and its offspring's continuous bag sleeving selfing separates, and orientation is selected precocity, high-quality, disease-resistant, long oval curcumbitate and the individual large individual plant material of melon, selects the good inbred line 06-1 of main stabilization characteristics of genetics through 12 generations.
Male parent and hybridization of female parent: be specially wholely and select irrigation and drainage convenience, the smooth plot of physical features to make multiplication fields, every 667m
2execute agriculture fertile 1000 kg, nitrophosphate fertilizer 25kg, potassium dihydrogen phosphate 5kg; Deep plough, rake, by line-spacing, 3m makes groove, with spade, scrapes out the groove lower than ground 15cm, is convenient to irrigate.By male parent inbred line 04-9 and maternal 06-1 field planting, at groove, the male parent field planting is at two ends, the pollination of being convenient to pick flowers; Male parent, maternal spacing in the rows are 0.5m; Every 667m
2maternal 320 strains of field planting, male parent 40 strains, male parent is capable of being 1:8 with female parent; After field planting, spray 2000-3000 times of liquid of 5% Imidacloprid missible oil and prevent eliminating aphis; Water.
When hybrid seeding is specially initial bloom stage, in conjunction with training, keeps down the vines of a creeping plant all male flowers in maternal strain are beaten only, guarantee to open without male flower in the maternal field of pollination period, retain all male flowers on paternal plant, extract all female flowers or young fruit; During pollination, noon before that day in male parent, maternal field, selects respectively and the mark second day is about to open male parent male flower and maternal female flower, use the knitting wool embroider, second day takes off the female knitting wool of taking, and strip off female flower corolla, take the male parent male flower gently, remove corolla and expose flower pesticide, male flower pollen is put on female chapiter equably, then with knitting wool, the female flower corolla is hitched, a general male flower can be awarded 3-5 female flower; 35-40 days after pollination gathers when fruit starts painted maturation, collects the squash hybridization seed.
embodiment 2
Be specially wholely and select irrigation and drainage convenience, the smooth plot of physical features to make multiplication fields, every 667m
2execute agriculture fertile 1000kg, nitrophosphate fertilizer 25kg, potassium dihydrogen phosphate 5kg; Deep ploughing, rake, by line-spacing, 3m makes groove, the squash hybridization seed that field planting embodiment 1 is prepared, and after field planting, the cultivation of watering of more solito pumpkin cultivation method.Sowing is to just receiving 105 days spring, 92 days autumns, 120 days spring of the time of infertility, 105 days autumns.Institute's pumpkin enduring store property that obtains is good, and the good and undamaged melon of the maturity place, rear normal temperature cool place that gathers can store more than 50 days.
Describe the present invention although above in detail with the preferred embodiment of the present invention; but be to be understood that; in the situation that do not deviate from the spirit and scope of the present invention that claims limit, the various distortion that those skilled in the art make and change are equally in protection scope of the present invention.